検索結果 800 件
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Okayasu, Mitsuhiro| Tsuchiya, Yuki| |
| キーワード | CFRP Carbon fiber Tensile strength Fatigue strength Low temperature |
| 発行日 | 2019-10-14 |
| 出版物タイトル | Journal of Science: Advanced Materials and Devices |
| 巻 | 4巻 |
| 号 | 4号 |
| 出版者 | Vietnam National University |
| 開始ページ | 577 |
| 終了ページ | 583 |
| ISSN | 24682179 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 Publishing services by Elsevier B.V. on behalf of Vietnam National University, Hanoi. |
| 論文のバージョン | publisher |
| DOI | 10.1016/j.jsamd.2019.10.002 |
| Web of Science KeyUT | 000504059200011 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jsamd.2019.10.002 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Shoji, Masaki| Ueda, Masako| Nishioka, Megumi| Minato, Hiroki| Seki, Masahide| Harada, Kenichi| Kubo, Miwa| Fukuyama, Yoshiyasu| Suzuki, Yutaka| Aoyama, Eriko| Takigawa, Masaharu| Kuzuhara, Takashi| |
| キーワード | CCN signaling pathway CCN2 Jiadifenolide Neurotrophin iPS |
| 発行日 | 2019-11-05 |
| 出版物タイトル | Biochemical and Biophysical Research Communications |
| 巻 | 519巻 |
| 号 | 2号 |
| 出版者 | Elsevier |
| 開始ページ | 309 |
| 終了ページ | 315 |
| ISSN | 0006291X |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 The Authors. Published by Elsevier Inc. |
| 論文のバージョン | publisher |
| PubMed ID | 31506177 |
| DOI | 10.1016/j.bbrc.2019.09.003 |
| Web of Science KeyUT | 00506404800015 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.bbrc.2019.09.003 |
| フルテキストURL | HP110_5_2_S81.pdf Fig.pdf Table.pdf |
|---|---|
| 著者 | Yumoto, Yasuhiro| Okada, Shigeru| Kinno, Ikuo| Nagamatsu, Tomohiro| Nouso, Kazuhiro| Nakayama, Eiichi| |
| キーワード | operational topics waste low-level waste disposal waste management |
| 発行日 | 2016-05 |
| 出版物タイトル | Health Physics |
| 巻 | 110巻 |
| 号 | 5 S2号 |
| 出版者 | LWW |
| 開始ページ | S81 |
| 終了ページ | S87 |
| ISSN | 0017-9078 |
| NCID | AA00662510 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 27023155 |
| DOI | 10.1097/HP.0b013e318254aef6 |
| Web of Science KeyUT | 000373920200006 |
| 関連URL | isVersionOf https://doi.org/10.1097/HP.0b013e318254aef6 |
| フルテキストURL | NanoLett_19_9_5915.pdf |
|---|---|
| 著者 | Yokoya, T.| Terashima, K.| Takeda, A.| Fukura, T.| Fujiwara, H.| Muro, T.| Kinoshita, T.| Kato, H.| Yamasaki, S.| Oguchi, T.| Wakita, T.| Muraoka, Y.| Matsushita, T.| |
| キーワード | Dopant local structure asymmetric dopant incorporation diamond dopant-vacancy complex photoelectron holography substitutional doping |
| 備考 | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Nano Letters, copyright © American Chemical Society after peer review and technical editing by the publisher.To access the final edited and published work see https://doi.org/10.1021/acs.nanolett.9b01481.| |
| 発行日 | 2019-08-02 |
| 出版物タイトル | Nano Letters |
| 巻 | 19巻 |
| 号 | 9号 |
| 出版者 | American Chemical Society |
| 開始ページ | 5915 |
| 終了ページ | 5919 |
| ISSN | 15306984 |
| NCID | AA11511812 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 31373825 |
| DOI | 10.1021/acs.nanolett.9b01481 |
| Web of Science KeyUT | 000486361900011 |
| 関連URL | isVersionOf https://doi.org/10.1021/acs.nanolett.9b01481 |
| フルテキストURL | PPaP_12_12_1338.pdf |
|---|---|
| 著者 | Ueda, Masashi| Yamagami, Daiki| Watanabe, Keiko| Mori, Asami| Kimura, Hiroyuki| Sano, Kohei| Saji, Hideo| Ishikawa, Kenji| Hori, Masaru| Sakakita, Hajime| Ikehara, Yuzuru| Enomoto, Shuichi| |
| キーワード | hemostasis imaging inflammation non-thermal plasma positron emission tomography |
| 発行日 | 2015-09-28 |
| 出版物タイトル | Plasma Processes and Polymers |
| 巻 | 12巻 |
| 号 | 12号 |
| 開始ページ | 1338 |
| 終了ページ | 1342 |
| ISSN | 16128850 |
| NCID | AA11976237 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| DOI | 10.1002/ppap.201500099 |
| Web of Science KeyUT | 000368449900002 |
| 関連URL | isVersionOf https://doi.org/10.1002/ppap.201500099 |
| フルテキストURL | AnnGastroenterolSurg_3_4_396.pdf |
|---|---|
| 著者 | Fujiwara, Toshiyoshi| |
| キーワード | adenovirus clinical trial esophageal cancer radiotherapy telomerase |
| 発行日 | 2019-07-05 |
| 出版物タイトル | Annals of gastroenterological surgery |
| 巻 | 3巻 |
| 号 | 4号 |
| 出版者 | John Wiley & Sons |
| 開始ページ | 396 |
| 終了ページ | 404 |
| ISSN | 24750328 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 The Authors |
| 論文のバージョン | publisher |
| PubMed ID | 31346579 |
| DOI | 10.1002/ags3.12270 |
| Web of Science KeyUT | 000475745100009 |
| 関連URL | isVersionOf https://doi.org/10.1002/ags3.12270 |
| フルテキストURL | J_ComputChem_39_4_202.pdf SI_J_ComputChem_39_4_202.pdf |
|---|---|
| 著者 | Sumi, Tomonari| Maruyama, Yutaka| Mitsutake, Ayori| Mochizuki , Kenji| Koga, Kenichiro| |
| キーワード | 3D-RISM theory chignolin classical density functional theory high-pressure unfolding hydrophobic solute protein temperature and pressure dependences of solvation free energy thermal denaturation |
| 発行日 | 2017-11-08 |
| 出版物タイトル | Journal of Computational Chemistry |
| 巻 | 39巻 |
| 号 | 4号 |
| 出版者 | Wiley |
| 開始ページ | 202 |
| 終了ページ | 217 |
| ISSN | 01928651 |
| NCID | AA00257341 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 29116647 |
| DOI | 10.1002/jcc.25101 |
| Web of Science KeyUT | 000417850400002 |
| 関連URL | isVersionOf https://doi.org/10.1002/jcc.25101 |
| フルテキストURL | J_ChemPhys_144_22_224104.pdf |
|---|---|
| 著者 | Sumi, Tomonari| Maruyama, Yutaka| Mitsutake, Ayori| Koga, Kenichiro| |
| 発行日 | 2016-06-10 |
| 出版物タイトル | Journal of Chemical Physics |
| 巻 | 144巻 |
| 号 | 22号 |
| 出版者 | American Institute of Physics |
| 開始ページ | 224104 |
| ISSN | 00219606 |
| NCID | AA00694991 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 27305993 |
| DOI | 10.1063/1.4953191 |
| Web of Science KeyUT | 000378926100007 |
| 関連URL | isVersionOf https://doi.org/10.1063/1.4953191 |
| フルテキストURL | PhysChemChemPhys_16_46_25492.pdf |
|---|---|
| 著者 | Sumi, Tomonari| Imamura, Hiroshi| Morita, Takeshi| Isogai, Yasuhiro| Nishikawa, Keiko| |
| 発行日 | 2014-10-17 |
| 出版物タイトル | Physical Chemistry Chemical Physics |
| 巻 | 16巻 |
| 号 | 46号 |
| 出版者 | Royal Society of Chemistry |
| 開始ページ | 25492 |
| 終了ページ | 25497 |
| ISSN | 14639076 |
| NCID | AA11301773 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 25343423 |
| DOI | 10.1039/c4cp03606a |
| Web of Science KeyUT | 000344989500036 |
| 関連URL | isVersionOf https://doi.org/10.1039/c4cp03606a |
| フルテキストURL | J_MoleculLiq_200_42.pdf |
|---|---|
| 著者 | Sumi, Tomonari| Imamura, Hiroshi| Morita, Takeshi| Nishikawa, Keiko| |
| キーワード | Protein solutions Lysozyme Protein-protein interaction Liquid state theory Integral equation DLVO model |
| 発行日 | 2014-04-01 |
| 出版物タイトル | Journal of Molecular Liquids |
| 巻 | 200巻 |
| 号 | Part.A号 |
| 出版者 | Elsevier Science |
| 開始ページ | 42 |
| 終了ページ | 46 |
| ISSN | 01677322 |
| NCID | AA10636062 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| DOI | 10.1016/j.molliq.2014.03.014 |
| Web of Science KeyUT | 000347592900010 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.molliq.2014.03.014 |
| フルテキストURL | RSC_Advances_2013_3_12743.pdf |
|---|---|
| 著者 | Sumi, Tomonari| Sekino, Hideo| |
| 発行日 | 2013-05-07 |
| 出版物タイトル | RSC Advances |
| 巻 | 3巻 |
| 号 | 31号 |
| 出版者 | Royal Society of Chemistry |
| 開始ページ | 12743 |
| 終了ページ | 12750 |
| ISSN | 20462069 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | publisher |
| DOI | 10.1039/C3RA41320A |
| Web of Science KeyUT | 000321791200034 |
| 関連URL | isVersionOf https://doi.org/10.1039/C3RA41320A |
| JaLCDOI | 10.18926/AMO/57370 |
|---|---|
| フルテキストURL | 73_5_403.pdf |
| 著者 | Ando, Akemi| Mitsuhashi, Toshiharu| Honda, Mitsugi| Hanayama, Yoshihisa| Hasegawa, Kou| Obika, Mikako| Kataoka, Hitomi| Otsuka, Fumio| |
| 抄録 | Osteoporosis increases the risk of bone fractures. It is diagnosed based on an individual’s bone mineral density (BMD) or a fracture without trauma. BMD is usually measured by the dual energy X-ray absorptiometry (DXA) method. Here we investigated factors for the earliest possible prediction of decreased BMD by examining the relationships between patients’ BMD values and changes in the patients’ physical and laboratory values. We retrospectively reviewed the medical records of 149 patients who visited our department in 2014-2015 for a variety of reasons and underwent an area BMD examination by DXA. We analyzed the relationships between decreasing BMD and the patients’ gender, age, body mass index (BMI), medical background, hemoglobin, electrolytes, and thyroid function. Thirty-nine of the patients were diagnosed with osteoporosis based on their T-scores. An adjusted analysis showed that female gender, aging, and increased serum calcium level were significantly related to decreasing femoral BMD, whereas high BMI was associated with an increase in femoral BMD. Collectively the results indicate that for the early detection of low BMD, it is important for general-practice physicians to consider conducting a BMD checkup when treating female and elderly patients with a low BMI and/or elevated serum calcium level. |
| キーワード | bone mineral density (BMD) body mass index (BMI) female gender hypercalcemia osteoporosis |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2019-10 |
| 巻 | 73巻 |
| 号 | 5号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 403 |
| 終了ページ | 411 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2019 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 31649366 |
| Web of Science KeyUT | 000491886600005 |
| JaLCDOI | 10.18926/AMO/57369 |
|---|---|
| フルテキストURL | 73_5_393.pdf |
| 著者 | Yi Yi Cho Thein| Win, Myitzu| Thuzara, Moe| Matsumoto, Hiroshi| Yamada, Kiyoshi| Kimata, Yoshihiro| Leung, Michael| |
| 抄録 | Although many surgical centers perform microsurgery routinely in developed countries, performing microsurgery is challenging in resource-poor developing countries, such as Myanmar. With the establishment of educational training programs and the assistance of volunteer plastic surgical teams, local plastic surgeons can learn the techniques of microsurgery and apply them clinically. The purpose of this study was to establish baseline data and define the challenges of performing microsurgery in Yangon General Hospital, Myanmar. Sixty-four patients underwent reconstruction with free flaps from January 2015 to January 2018. All clinical records of these cases were assessed. The number of free flap reconstructions performed increased from 11 in the first year to 24 in the third year. The anterolateral thigh flap was the most commonly used (42%). The most common sites of reconstruction were mandible and intraoral defects. Total flap survival occurred in 58 of 64 patients (89%). The total salvageable flap rate for revision surgery was 66.6%; the successful revision rate was highest in 2017, with fewer complications. The flap salvage rates increased and the operative duration decreased as clinical experience improved. Establishing a microsurgical center requires a strong multidisciplinary team, clinical experience, continuous learning, sensible clinical application, and effective interdepartmental and intradepartmental cooperation. |
| キーワード | microsurgery educational programs challenges of microsurgical free flaps reoperation flap salvageable rate |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2019-10 |
| 巻 | 73巻 |
| 号 | 5号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 393 |
| 終了ページ | 401 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2019 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 31649365 |
| Web of Science KeyUT | 000491886600004 |
| JaLCDOI | 10.18926/AMO/57367 |
|---|---|
| フルテキストURL | 73_5_383.pdf |
| 著者 | Fu, Li| Nishibori, Masahiro| |
| 抄録 | High mobility group box-1 (HMGB1) is a non-histone, DNA-binding nuclear protein belonging to the family of damage-associated molecular patterns (DAMPs). HMGB1 has been reported to play an important role during epileptogenesis although the mechanisms of its actions are still not clear. Many hypotheses have been suggested especially about the relationship between HMGB1 and inflammation responses and blood-brain barrier disruption during epileptogenesis. In this review, we will mainly discuss the role of HMGB1 in epileptogenesis. |
| キーワード | HMGB1 epileptogenesis inflammation blood-brain barrier |
| Amo Type | Review |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2019-10 |
| 巻 | 73巻 |
| 号 | 5号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 383 |
| 終了ページ | 386 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2019 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 31649363 |
| Web of Science KeyUT | 000491886600002 |
| JaLCDOI | 10.18926/AMO/57366 |
|---|---|
| フルテキストURL | 73_5_379.pdf |
| 著者 | Wake, Hidenori| |
| 抄録 | Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 μg/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi. Therefore, reduction of plasma HRG levels in sepsis leads to dysregulation of coagulation, fibrinolysis, and immune response, resulting in disseminated intravascular coagulation and multiple organ failure. This review summarizes the binding and functional properties of HRG in sepsis. |
| キーワード | htidine-rich glycoprotein septic pathogenesis immunothrombosis |
| Amo Type | Review |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2019-10 |
| 巻 | 73巻 |
| 号 | 5号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 379 |
| 終了ページ | 382 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2019 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 31649362 |
| Web of Science KeyUT | 000491886600001 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Janisch, Florian| Abufaraj, Mohammad| Fajkovic, Harun| Kimura, Shoji| Iwata, Takehiro| Nyirady, Peter| Rink, Michael| Shariat, Shahrokh F.| |
| キーワード | Disease management Primary urethral carcinoma Prognosis Therapy Treatment |
| 発行日 | 2019-07-13 |
| 出版物タイトル | European Urology Focus |
| 巻 | 5巻 |
| 号 | 5号 |
| 出版者 | Elsevier |
| 開始ページ | 722 |
| 終了ページ | 734 |
| ISSN | 24054569 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 European Association of Urology. Published by Elsevier B.V. |
| 論文のバージョン | publisher |
| PubMed ID | 31307949 |
| DOI | 10.1016/j.euf.2019.07.001 |
| Web of Science KeyUT | 000490140000008 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.euf.2019.07.001 |
| フルテキストURL | J_Thorac_Oncol_201907017.pdf |
|---|---|
| 著者 | Makimoto, Go| Ohashi, Kadoaki| Tomida, Shuta| Nishii, Kazuya| Matsubara, Takehiro| Kayatani, Hiroe| Higo, Hisao| Ninomiya, Kiichiro| Sato, Akiko| Watanabe, Hiromi| Kano, Hirohisa| Ninomiya, Takashi| Kubo, Toshio| Rai, Kammei| Ichihara, Eiki| Hotta, Katsuyuki| Tabata, Masahiro| Toyooka, Shinichi| Takata, Minoru| Maeda, Yoshinobu| Kiura, Katsuyuki| |
| キーワード | ALK G1202R Alectinib Amphiregulin MET NSCLC |
| 発行日 | 2019-07-30 |
| 出版物タイトル | Journal of Thoracic Oncology |
| 巻 | 14巻 |
| 号 | 11号 |
| 出版者 | Elsevier |
| 開始ページ | 2009 |
| 終了ページ | 2018 |
| ISSN | 15560864 |
| NCID | AA12058455 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 International Association for the Study of Lung Cancer. Published by Elsevier Inc. All rights reserved. |
| 論文のバージョン | author |
| PubMed ID | 31374369 |
| DOI | 10.1016/j.jtho.2019.07.017 |
| Web of Science KeyUT | 000492678300025 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.jtho.2019.07.017 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Hashimoto, Hideki| Muramatsu, Yusuke| Nishina, Yuta| Asoh, Hidetaka| |
| キーワード | Graphite Graphene Graphene oxide Electrochemical exfoliation Anode Bipolar electrochemistry |
| 発行日 | 2019-07-31 |
| 出版物タイトル | Electrochemistry Communications |
| 巻 | 104巻 |
| 出版者 | Elsevier |
| 開始ページ | 106475 |
| ISSN | 13882481 |
| NCID | AA11324699 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 The Authors. Published by Elsevier B.V. |
| 論文のバージョン | publisher |
| DOI | 10.1016/j.elecom.2019.06.001 |
| Web of Science KeyUT | 000480667900012 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.elecom.2019.06.001 |
| フルテキストURL | Blood advances 2018.pdf |
|---|---|
| 著者 | Inamoto, Yoshihiro| Matsuda, Tomohiro| Tabuchi, Ken| Kurosawa, Saiko| Nakasone, Hideki| Nishimori, Hisakazu| Yamasaki, Satoshi| Doki, Noriko| Iwato, Koji| Mori, Takehiko| Takahashi, Satoshi| Yabe, Hiromasa| Kohno, Akio| Nakamae, Hirohisa| Sakura, Toru| Hashimoto, Hisako| Sugita, Junichi| Ago, Hiroatsu| Fukuda, Takahiro| Ichinohe, Tatsuo| Atsuta, Yoshiko| Yamashita, Takuya| Japan Society for Hematopoietic Cell Transplantation Late Effects and Quality of Life Working Group| |
| 発行日 | 2018-08 |
| 出版物タイトル | Blood Advances |
| 巻 | 2巻 |
| 号 | 15号 |
| 出版者 | American Society of Hematology |
| 開始ページ | 1901 |
| 終了ページ | 1903 |
| ISSN | 2473-9529 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | publisher |
| PubMed ID | 30087108 |
| DOI | 10.1182/bloodadvances.2018020966 |
| 関連URL | isVersionOf https://doi.org/10.1182/bloodadvances.2018020966 |
| JaLCDOI | 10.18926/AMO/56933 |
|---|---|
| フルテキストURL | 73_4_315.pdf |
| 著者 | Ono, Shintaro| Nakayama, Masaaki| Tachibana, Masato| Abu Saleh Muhammad Shahriar| Heling, Wang| Takashiba, Shogo| Ohara, Naoya| |
| 抄録 | The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism’s virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains. |
| キーワード | periodontitis Porphyromonas gingivalis gingipain C-terminal domain secretion system |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2019-08 |
| 巻 | 73巻 |
| 号 | 4号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 315 |
| 終了ページ | 323 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2019 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 31439954 |