result 1646 件
| JaLCDOI | 10.18926/11776 |
|---|---|
| Title Alternative | Coping for the better quality of life(QOL) after adult kidney transplantation and factors affecting on the coping |
| FullText URL | 007_1_049_057.pdf |
| Author | Hayashi, Yuko| |
| Abstract | 腎移植は、免疫抑制剤の進歩に伴う高い生存率や生着率はもちろんのこと、今日では特に移植後のQOLに焦点が当てられている。移植後のQOLを向上させていくためには、レシピエントが移植後の様々な出来事にいかに対処していくかが重要である。本研究は、腎移植後のレシピエントの対処に焦点を当て、移植後のQOLに関係する対処および対処に影響を及ぼす要因を明らかにすることを目的とした。対象者は、関東の2施設で腎移植を受けて入院および外来通院をしている20才以上の10名のレシピエントで、研究参加の同意を得た後、腎移植後どのように対処しているかや、何が対処に影響を与えているかについて面接並びに観察による調査を行った。分析の結果、腎移植後の対処は＜問題と取り組む＞＜情報の交換＞＜問題状況の再認知＞である対処様式が主に用いられており、医師に任せるという＜お任せ＞は全員に見られなかった。また、腎移植後の対処に影響を及ぼす要因として、＜身体の状態＞＜自己概念＞＜不確かさ＞＜ソーシャルサポート＞が見いだされた。 |
| Keywords | 対処 (coping) 身体の状態 (physical state) 自己概念 (self concept) 不確かさ (uncertainty) ソーシャルサポート (social support) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 49 |
| End Page | 57 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313679 |
| JaLCDOI | 10.18926/11774 |
|---|---|
| Title Alternative | The health and daily-life problems of the elderly living in their own homes for six months after being discharged from the hospital |
| FullText URL | 007_1_041_047.pdf |
| Author | Ohta, Niwa| Inoshita, Hikari| Ikeda, Toshiko| Nakanishi, Yoshiko| Kondo, Masuko| Takata, Setuko| Kojima, Mishako| |
| Abstract | 病院を退院した高齢者の療養上の問題を把握することは、退院指導や継続看護を進める上でも重要である。著者らは退院時、退院後3か月、6か月、1年のそれぞれの時点で、在宅での看護上の問題について調査研究を行い、すでにこれらの一部は報告した。今回は退院後6ヵ月を経過した高齢者の在宅療養時の健康及び日常生活の問題について報告する。総合病院に入院していた70歳以上の高齢者で、退院時に調査を行った93名のうち、退院後6ヵ月の時点で回答を得た60名（64.5％）について分析した。調査は郵送法により、調査内容は健康状態、自立度、困っていること等で、結果は退院時の状態と比較・検討した。退院6か月後の日常生活の自立度がよい者の割合は退院時より8.4％増加し、体の調子が「良くなった」とする者は50％を越えた。しかし、困っていることとして「健康面」の問題をあげた者は23.3％と高く、退院時の2倍になっていた。また退院時の問題点の70％はなお持続しており、食事療法・移動動作・下肢痛・視聴覚障害・排泄障害・気管支炎等の順に多かった。6ヶ月後の新たな問題として注目すべきものとしては人間関係やコミュニケションの障害が認められた。 |
| Keywords | 高齢者 (the elderly) 自宅退院 6か月後 (six months after discharge from the hospital) 健康問題 (health) 日常生活 (daily-life problems) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 41 |
| End Page | 47 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313479 |
| JaLCDOI | 10.18926/11773 |
|---|---|
| FullText URL | 001_069_076.pdf |
| Author | Sasaki Kenji| Hirota Takashi| Iwado Akimasa| Hirota Kazuhiro| |
| Abstract | Reactions of cyclic ketones such as α-tetralone, 1,3-cyclohexanedione, or naphthalenedione with formamide or trisformylaminomethane (TFAM) have shown to form polyclic fused pyrimidines by us. Reactions of terpene ketones like l-menthone, d-camphor, l-carvone with TFAM were performed, and 8-isopropyl-5-methyl-5,6,7,8-tetrahydroquinazoline, borno[2,3-d] pyrimidine, and 5-isopropenyl-8-methyl-5,6-dihydroquinazoline were expectedly obtained from three terpenes. Minor products of 5-isopropenyl-8-methyl-5,6,7,8-tetrahydroquinazoline and 5-isopropenyl-8-methylquinazoline were formed with 5-isopropenyl-8-methyl-5,6-dihydroquinazoline by disproportionation reaction of l-carvone. Furthermore, No-formylmenthylamine, N-formylbornylamine, and N-formylcarvylamine were obtained as the Leuckart-type products terpene ketones in these reactions. The reaction of N-benzyl-4-piperidone with TFAM gave desired 6-benzyl-5,6,7,8-tetrahydropyrido[4,3-d]pyrimidine. The reaction of diethyl succinylsuccinate with TFAM afforded tricyclic 4,9-dioxo-3,4,8,9-tetrahydropyrimido[4,5-g]quinazoline. Above compounds were determined by the measurements of their instrumental analyses. |
| Keywords | Cyclic ketone Trisformylaminomethane Fused pyrimidine Cyclization Reductive amination |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 69 |
| End Page | 76 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313993 |
| JaLCDOI | 10.18926/11772 |
|---|---|
| Title Alternative | The influence on the family caused by the mother staying away from home in order to take care of a sick child being hospitalized -six cases of siblings left at home who refused to go to school because of this situation- |
| FullText URL | 007_1_035_039.pdf |
| Author | Ohta, Niwa| |
| Abstract | 母親が病気の子どもに1か月以上付き添う場合の家に残されている同胞への影響について研究を行ってきた。今回、その同胞172ケースのうちで登校拒否があった6ケースについて子どもの特徴、家族構成、家族状況等を検討した。その結果、6ケースのうち3ケースが9歳の女児であった。同居家族は1ケースで、他は核家族であった。この同胞たちは、「笑顔がなくなる」、「寂しそうな表情をする」など何らかの精神的不安を表していた。登校拒否の始まった時期は全て入院後1か月未満の時であった。登校拒否が一時的でなく、退院後にも長びいたケースは父親と女児のみが家に残されていた。このような結果から核家族が多い現在小児看護を行う場合、残された同胞への配慮を早期から行いながら病児や母親の援助を行うことが重要である。 |
| Keywords | 付き添い 長期入院 (hospitalization) 家族状況 (mother, family) 同胞 登校拒否 (refusing to go to school) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 35 |
| End Page | 39 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313617 |
| JaLCDOI | 10.18926/11771 |
|---|---|
| Title Alternative | Factors Affecting Home Care of Elders Report 1. Support Expected by the Elderly and Family |
| FullText URL | 001_061_068.pdf |
| Author | Ono, Tsuruko| Arakawa, Yasuko| Ohta, Niwa| Nanba, Jun| Ohta, Takeo| Shimizu, Masako| Ishii, Kiyoko| |
| Abstract | For the rapid increase of the elders in population in Japan after the 2nd world war, the arrangement of medical care for aged persons, both therapeutic and preventive, has become the serious social problem. To estimate the possibility of care at home and needs of elders and their family for public and volunteer services, questionnaire survey was carried out at Konko town in Okayama prefecture, Japan, in August 1990. More than half of the 105 elders older than 65 years and living alone (group A, 9 males and 96 females) replied to want to be cared for at home and 178 families with elders (group B) wanted to care for them at home. As for services they would wish to be offered when they would become bedridden at home, while group A wished to be visited and cared for by 'physician', 'home helper' and 'neighbor' in order of high rate group B 'physician', 'public health nurse' and 'nurse'. As for facility or assistance services, the former wanted 'purchasing, sweeping and washing', food delivery' and 'calling on' and the latter 'care consultation', 'assist of body bath' and 'economic aid'. Other supports or cares were also hopefully expected by both groups. The results shows that well-arrangement and promotion of a variety of constitional and personal support for home cares for elders in the community must be urged. |
| Keywords | Home Care Family Care the Elderly Support |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 61 |
| End Page | 68 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313467 |
| JaLCDOI | 10.18926/11770 |
|---|---|
| Title Alternative | A study on students' idears about the school syllabus |
| FullText URL | 007_1_029_033.pdf |
| Author | Ohta, Takeo| Shimoishi, Yasuaki| Azuma, Yoshiharu| Ichimura, Mituko| Endo, Hiroshi| Okazaki, Yuka| |
| Abstract | 1995年4月に初めての本学のシラバス（講義要覧）が発行され、学生に配布された。本報は約1年後の1996年2月の試験期間中にシラバスに対する学生の意見を問うために行われたアンケート調査の結果をまとめたものである。回答を寄せた学生は435人で、在籍学生の89.0％であった。よく利用したとする学生は18.2％、数回利用したとする学生は67.6％であった。学生の使用目的はテストの準備や履修科目の決定のためであった。以上のことから、現在のシラバスは学生が日常的により活用するためにはさらに教師が改善、工夫する必要があると思われた。 |
| Keywords | シラバス (syllabus) 授業計画 講義要覧 学生の意見 (student) use |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 29 |
| End Page | 33 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313997 |
| JaLCDOI | 10.18926/11769 |
|---|---|
| Title Alternative | The Study of Nursing Care of Dying Patients and Their Families - Ritual Behaviors and Wordings of Nurses - |
| FullText URL | 001_051_060.pdf |
| Author | Takata, Setuko| |
| Abstract | The purpose of this study was to obtain the basic data for the study of "Nursing care of dying patients and their families". I investigated 59 nurses working at hospitals in Tokushima know their ritual behaviors and their wordings that nurses and their families made for dying patients at moment of their death in the hospital. The results were; 1) Typically, two ritual behaviors were reported at attending patient's death. One was "Tamayobi"; calling dying patient's name to commend his soul to God or Hotoke. Other was "Matugo no mizu", giving water to dying patient to sympathy him. Few nurses didn't know "Tamayobi". Many nurses knew "Mstugo no mizu", however, only a few nurses did it in the hospital. 2) Nurses talked with their families in many ways around their last hours. This wordings included not only to explain dying patient's care, but also to alleviat the grief of their families much more. |
| Keywords | Tamayobi Matugo no mizu |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 51 |
| End Page | 60 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313482 |
| JaLCDOI | 10.18926/11766 |
|---|---|
| Title Alternative | Basic properies of beam flatness analyzer (THEBES 7000) |
| FullText URL | 007_1_023_027.pdf |
| Author | Nakagiri, Yoshitada| Oguri, Nobuhiro| Maruyama, Toshinori| Okawa, Yoshihiro| Wakasa, Hiroyuki| Nagaya, Isao| Azuma, Yoshiharu| Shibuya, Koichi| Goto, Sachiko| Mikami, Yasutaka| Inamura, Keiji| Morioka, Yasuki| Sugita, Katsuhiko| Hiraki, Yoshio| |
| Abstract | 癌の有力な治療法の1つである放射線治療はシステム全体の許容誤差が±5％と、正確な治療が要求されている。正確な線量を正確な場所に照射されなければならない。そのためには日頃から照射装置のQuality Control が重要である。Beam平坦度の測定はその中でも、重要な項目で1／6月の測定頻度が勧告されている。今回、市販のBeam Flatness Analyzer (7000型 THEBES) を使用する機会を得たので、その基礎的性能であるChamber 相互の感度のちがい、測定値の再現性、識別可能な最小線量について実験を行った。各項目とも誤差が非常に少なく、日常の使用に際してそのデータは十分信頼できるものであることがわかった。 |
| Keywords | 放射線治療 (radiation therapy) ビーム平坦度 (beam flatness) 性能評価 (valuation of property) 電子加速器 (electron accelerator) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 23 |
| End Page | 27 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313499 |
| JaLCDOI | 10.18926/11763 |
|---|---|
| Title Alternative | Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis |
| FullText URL | 001_039_050.pdf |
| Author | Nakata, Yasunari| |
| Abstract | Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed. |
| Keywords | sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 39 |
| End Page | 50 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313772 |
| JaLCDOI | 10.18926/11760 |
|---|---|
| Title Alternative | Alteration of thermostable phosphatase activity after hydrophobic chromatography |
| FullText URL | 007_1_017_021.pdf |
| Author | Mori, Shuji| Okamoto, Motoi| Nakata, Yasunari| Endo, Hiroshi| |
| Abstract | 耐熱性ホスファターゼを含んだBacillus stearothermophilus 粗酵素試料を、リソースIsoによる疎水性クロマトグラフィにかけ分離を行った。1.5M→0M 硫酸アンモニウムの直線逆濃度勾配によって溶出を行ったところ、ホスファターゼは不活性な形で溶出され、これは硫酸アンモニウムによる濃度依存的阻害に起因することが判明した。ホスタファーゼの反応混合液に種々の濃度の硫酸アンモニウムを添加したところ、0.15Mの硫酸アンモニウム存在下で約80％の阻害が認められた。加えて、この阻害作用は単に硫酸アンモニウムの添加によってpHが酸性側に傾くことによるものではないことも明らかとなった。 |
| Keywords | 耐熱性ホスファターゼ (thermophilic phosphatase) 疎水性クロマトグラフィ (hydrophobic chromatography) 硫酸アンモニウム (ammonium sulfate) 濃度依存的阻害 (dose- dependent inhibition) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-08-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 17 |
| End Page | 21 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002314041 |
| JaLCDOI | 10.18926/11759 |
|---|---|
| Title Alternative | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| FullText URL | 007_1_009_015.pdf |
| Author | Sakiyama, Junko| Ichimura, Mitsuko| Tohge, Hiroko| Endo, Hiroshi| Kawakami, Kaori| Kawatani, Yuki| |
| Abstract | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| Keywords | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 9 |
| End Page | 15 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11758 |
|---|---|
| Title Alternative | Literature review on the adult kidney transplantation - based on nursing perspective- |
| FullText URL | 007_1_001_007.pdf |
| Author | Hayashi, Yuko| |
| Abstract | 慢性腎不全の治療法の一つに腎移植がある。この文献検討の目的は、医学、精神医学、心理学、社会学ならびに看護学における腎移植に関する諸外国を含めた最近の研究論文を吟味し、今後の腎移植看護の実践や研究に必要な課題を明らかにすることである。文献検討は、看護学の立場から重要と思われる腎移植を受けた成人を対象にした研究論文を集めて、それを基に行った。その結果、腎移植は人工透析やCAPDよりもQOLを高める治療法であり、慢性腎不全患者にとっては期待が大きく関心の高い治療法であることが明確になった。また移植後に新たな身体的、心理的、精神的及び社会的な問題が生じることによって、レシピエントはストレスフルな状況にさらされやすいこと、レシピエントの効果的な対処の仕方がQOLを高めることなどがわかった。これらの知見から、腎移植看護の発展のためには、腎移植者の生活体験の意味を明らかにしたり、効果的な看護介入・教育の開発をめざした研究や、移植前から移植後を通じての継続的な看護の取り組みが今後の課題として考えられた。 |
| Keywords | 移植医療 腎移植 (kidney transplantation) QOL (quality of life) 対処 (coping) 腎移植看護 (nursing intervention) patient education |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-09-30 |
| Volume | volume7 |
| Issue | issue1 |
| Start Page | 1 |
| End Page | 7 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313915 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| Title Alternative | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| FullText URL | 001_029_037.pdf |
| Author | Yamamoto, Goki| |
| Abstract | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| Keywords | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 29 |
| End Page | 37 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11756 |
|---|---|
| Title Alternative | 大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 |
| FullText URL | 006_063_072.pdf |
| Author | Ito, Sekiko| Okamoto, Motoi| |
| Abstract | Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA fragments inserted into the expression vector (pGEX-4T-1) were amplified by RT-PCR using mRNA purified from E18 rat brain as template. Primers attached with BamH I or EcoR I restriction site on 5' end were used to amplify first strand cDNA by PCR. Before ligation into the pGEX-4T-1 for GST fusion protein, PCR products were once cloned using T-A cloning system because they were not directly ligated into the pGEX-4T-1. E.coli strain BL21 was transformed by pGEX-4T-1 ligated with restriction DNA fragment cut out from pCR II plasmid vector of T-A clonig system. The growth of transformed BL21 was not different between the colony incubated at 37℃ for 24-48h and the colony stored at 4℃ for 7-10 days after 24h incubation at 37℃. The desirable OD(550) of culture medium for inducing the expression of fusion protein by isopropylthio-β-D-galactoside (IPTG) was from 0.6 to 1.0, because expression of native E.coli proteins per ml of culture medium was increased relatively when IPTG was added at OD(550) more than 1.0. The expression of fusion protein reached plateau around 6h after the induction. Relative expression of native E.coli proteins per ml of culture medium increased thereafter. Therefore, it may be desirable to purify the fusion protein around 6h after the induction. |
| Keywords | phosphacan (フォスファカン) glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) BL21 IPTG fusion protein (融合蛋白) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 63 |
| End Page | 72 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002313693 |
| JaLCDOI | 10.18926/11755 |
|---|---|
| Title Alternative | New Stepwedge for Bootstrap Sensitometry in Medical Radiography |
| FullText URL | 001_019_027.pdf |
| Author | Yoshida, Akira| Kobashi, Toshimi| Ohkawa, Yoshihiro| Mikami, Yasutaka| Hiraki, Yoshio| Azuma, Yoshiharu| Yamada, Toshiharu| Sugita, Katsuhiko| |
| Abstract | A new stepwedge has been developed for bootstrap sensitometry, which is used for determining the characteristic curve of radiographic screen/film systems. Since each step of the stepwedge is separated by the lead septum, flare generated under one step will not penetrate the region of another step. The metal of the stepwedge can be changed with various materials. Characteristic curves obtained with new stepwedge bootstrap (NSB) and conventional stepwedge bootstrap (CSB) techniques were compared with those obtained for screen/film systems with inverse sqare sensitometry. The inverse square method was used as the reference standard for accuracy. The NSB method provided characteristic curves that agreed well with those obtained using the inverse square method. The slope of the curve in the CSB method was lower than that in the inverse square or NBS method, because of scatter radiation. The shape of the characteristic curve from the NSB method did not vary with the materials of step metals (aluminum and copper). The cumulative error involved when using the bootstrap method is also discussed. |
| Keywords | Stepwedge Bootstrap Characteristic curves Sensitometry Radiographic screen/film systems |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 19 |
| End Page | 27 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313394 |
| JaLCDOI | 10.18926/11754 |
|---|---|
| Title Alternative | On Club Activity in School -Especially Those Related with the Tokubetsu Katsudo- |
| FullText URL | 001_011_018.pdf |
| Author | Jin, Fumio| |
| Abstract | Judzing from this, club activities must be included not in school education but in adult education. And so club activities in school must be practiced from the point of adult education. It seems, however, that the accepter has not been prepard yet. For the reason, It is impossible to exclude club activities at once from school education. From this point, the system of life-long education must be made up promptly and club activities are preferable to be separated from school education. On both sides of school education and adult education, the administrative readjustment must be excuted first of all for the activities of school clubs being pushed forward. It seems, however, that the problems are in Shido-yoryo itself: especially how to deal with Tokubetsu-katsudo is the problem. In other words, Shido-yoryo says, "Tokubetsu-katsudo must be separated from adult education" or "It is not practiced necessarily in adult education" in one time and "It has been applied corresponding to Bukatsu instead of Kurabu" in another time. Shido-yoryo has influenced greatly on the activities in school education. |
| Keywords | 学校教育 学習指導要領 特別活動 クラブ活動 部活 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 11 |
| End Page | 18 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313632 |
| JaLCDOI | 10.18926/11753 |
|---|---|
| Title Alternative | The effects of various fixating buffer soution in the electron microscopic observations |
| FullText URL | 006_055_061.pdf |
| Author | Akatsuka, Kazuya| |
| Abstract | 生物学的あるいは生化学的な緩衝液を使用して作製された電子顕微鏡試料と従来の電子顕微鏡用として使用されている緩衝液で作成した試料とで電子顕微鏡像から比較検討した。HEPES緩衝液で作製した試料の電子顕微鏡像は、従来の電子顕微鏡に用いられている緩衝液で作製した試料から得られた電子顕微鏡像と非常によく似た像を示し、このHEPES緩衝液は電子顕微鏡の固定用緩衝液として使用できることが認められたが、その他（PIPES、MOPS緩衝液）の緩衝液ではあまりにも細胞内の可溶性物質が溶出しすぎて使用に耐えなかった。電子顕微鏡学研究において使用されうる固定用緩衝液は細胞内のオルガネラの保持が余りにも良い場合には、その微細構造の解析が困難になり、程々に細胞内の可溶性物質が溶出した方が解析がし易くなると思われ、この様な観点から、今回使用したHEPES緩衝液は生物学的あるいは生化学的な研究から引き続き電子顕微鏡用の試料を作製する際、同じ緩衝液が使用できることが認められた。 |
| Keywords | 電子顕微鏡 (electron microscopy) 微細構造 (ultrastructure) 肝細胞 (liver cell) カコジル酸緩衝液 (cacodylate buffer) HEPES緩衝液 (HEPES buffer) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 55 |
| End Page | 61 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313640 |
| JaLCDOI | 10.18926/11752 |
|---|---|
| FullText URL | 001_007_009.pdf |
| Author | Tongu Yasumasa| Bayoumi Magdi Abdel Hamid| Saida Haruo| |
| Abstract | マンソン住血吸虫とビルハルツ住血吸虫に混合感染し、しかも尿からマンソン住血吸虫の虫卵を検出した珍しい症例。患者は18才の男子でスーダンのハルツーム市在住。3年前に流行地で水に接する機会があった。最近、朝の最初の尿で血尿が出るようになった。また腰痛および吐気も自覚するようになった。1990年7月26日個人のクリニックで検査したところ尿の沈渣中より2種類の住血吸虫卵を検出した。スーダンでは混合感染者の3～5％に尿からマンソン住血吸虫卵が検出されるといわれているが確実な報告例はない。 |
| Keywords | Schistosoma mansoni S.haematobium schistosomiasis Sudan |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 7 |
| End Page | 9 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002314060 |
| JaLCDOI | 10.18926/11751 |
|---|---|
| Title Alternative | Nursing students' perception of their acquired basic skills in nursing practices : a comparison between a new and an old curriculum |
| FullText URL | 006_047_053.pdf |
| Author | Maeda, Makiko| Kondo, Masuko| Ohta, Niwa| Ikeda, Toshiko| Nakanishi, Yoshiko| Takabatake, Harumi| Ohi, Nobuko| Takata, Setuko| |
| Abstract | 平成2年度改正の新カリキュラムで学んだ学生と旧カリキュラムで学んで学生間に、卒業時の基礎看護技術の到達度に違いがあるかを明らかにするために、看護技術の基本的要素、日常生活の援助技術、診療時の援助技術の49項目の到達度について、3年制課程の3年生に質問紙調査を行った。対象者全体では、到達度の上位10位以内に日常生活の援助技術項目の37％が含まれ、到達度の下位10位以内に診療時の援助技術項目の37％が含まれていた。新カリキュラム群と旧カリキュラム群の比較では、21項目に有意差があった。新カリキュラム群の高い15項目は日常生活の援助技術と看護技術の基本的要素に関するものが多く、旧カリキュラム群が高い6項目は診療時の援助技術が多かった。カリキュラム改正に関係なく、日常生活の援助技術の到達度は高く診療時の援助技術は低い傾向にあったが、カリキュラム改正後はその傾向が強まり、さらに看護技術の基本的要素の到達度はカリキュラム改正後に比較的高くなっていることが明らかになった。新カリキュラムでは臨地実習時間を削減しているが、基礎教育で重視する日常生活の援助技術と看護技術の基本的要素は習得できていると考えられる。 |
| Keywords | 基礎看護技術 (the basic skills in nursing practices) 到達度の学生評価 (students' perception of their acquirement) カリキュラム改正 (curriculum revision) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1996-02-29 |
| Volume | volume6 |
| Start Page | 47 |
| End Page | 53 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313682 |
| JaLCDOI | 10.18926/11750 |
|---|---|
| Title Alternative | Total Skin Electron Beam Therapy |
| FullText URL | 001_001_006.pdf |
| Author | Nakagiri, Yoshitada| Inamura, Keiji| Miyake, Hideaki| Tahara, Seiji| Mimura, Seiichi| Egusa, Tomomi| Mikami, Yasutaka| Yamada, Toshiharu| Sugita, Katsuhiko| Hiraki, Yoshio| |
| Abstract | The peripherally T-cell lymphoma; Mycosis fungoides etc, has the good radiation sensitivity, and has been adapted for total skin electron beam therapy. In this study the pendular irradiation method was used for the purpose of total skin electron beam therapy in Mycosis fungoides, and physical data on the radiation field and the electron beam energy were useful clinically. |
| Keywords | 電子線全身照射法 菌状息肉症 全身性皮膚疾患 |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1991-03-25 |
| Volume | volume1 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 0917-4494 |
| language | Japanese |
| File Version | publisher |
| NAID | 120002313887 |
