| タイトル（別表記） | Studies on Root Fluorescence Mutants in Barley |
|---|---|
| フルテキストURL | 005_002_193_202.pdf |
| 著者 | 武田 和義| 張 成林| |
| 抄録 | Using 10,473 barley accessoins and 16,480 gamma-ray lines, root fluorescence mutants which have been reported in order crops, such as soybean, were screened. No spontaneous mutant was found,but an artificial mutant whose root tips emitted a pink glow under ultraviolet light was obtained by the gamma-ray irradiation. The mutant was controlled by a recessive gene named frp 'fluorescent reaction-pink'. It was poor growth and was lethal. The transmissoin rate of the mutant gene was about 80% of that of the normal allele. Thus the segregation in the heterozygous populations was ca. 4 normal to 1 mutant. The frp gene is located close to gl-3, 'glossy leaf-3' on the 4H chromosome. |
| キーワード | Barley Mutation Fluorescence Linkage analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 193 |
| 終了ページ | 202 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | QTL Analysis for Expressivity of Hull-cracked Grain in Two-rowde Spring Barley |
|---|---|
| フルテキストURL | 005_002_183_191.pdf |
| 著者 | 金谷 良市| 高橋 秀和| 武田 和義| |
| 抄録 | The exposure of the caryopsis through lemma and palea is called 'hull-cracked grain', which lowers the germinability and grade of malting barley. To breed new varieties with a low occurence of hull-cracked grains,quantitative trait loci(QTL) analysis was conducted using 146 doubled haploid lincs derived from Harrington × TR306.Interval mapping analysis revealed three significant QTLs on chromosomes 3H,5H and 7H. About 37% of the variation of the expressvity of hull-cracked grains was explained by these three QTLs in a spikelet-thinning condition where 1/4 of the spikelets were thinned at the flowering time. On the contraty the QTL on 5H was silent in normal or non-treated condition and 23% of the variation was determined by the QTLs on 3H and 7H. Some of the QTLs detected here had a pleiotropic effect on the grain size. |
| キーワード | Barley Quality Hull-cracked grain QTL analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 183 |
| 終了ページ | 191 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Vertical Structure of Temperatures of Tree's Leaves and Gate Wall at Rashomon Doline |
|---|---|
| フルテキストURL | 005_002_169_181.pdf |
| 著者 | 宮下 晃一| 米谷 俊彦| |
| 抄録 | Temperatures of tree's leaves and gate wall were measured in the summer of 1996 at doline. Diurnal fluctuations of leaf temperatures were greater, the higher the position of leaf in the doline, and they showed maximum temperatures later, the deeper the leaf position. The leaf temperature of trees growing in the first collapse doline was uniform in the horizontal direction and had a stable thermal layer. The difference between leaf temperatures at a depth of 20 m and 11 m was about 12℃ around noon. The leaf temperature was always higher at the upper part than at the lower part of the doline throughout the day. Daily fluctuation of wall temperature was small,compared with that of leaf temperature. The vertical profile of wall temperature was similar to that of leaf temperature. The warm water flowing down from the upper part of the doline influenced the soil temperature in the lower part of the doline. The thermal imagery elucidated the peculiar vertical structure of leaf and wall temperature formed in the summer at Rashomon doline. |
| キーワード | Rashomon Doline Leaf temperature Wall temperature Thermal image |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 169 |
| 終了ページ | 181 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Habenaria Mosaic Virus, A New Member of Potyvirus from Habenaria radiata Orchid |
|---|---|
| フルテキストURL | 005_002_155_168.pdf |
| 著者 | 井上 成信| 前田 孚憲| 光畑 興二| Gara I Wayan| |
| 抄録 | A potyvirus, isolated from Habenaria radiaata showing severe mosaic in the leaves named habenaria mosaic virus(HaMV). HaMV was transmitted by inoculation of sap to 6 of 41 species from 4 of 12 families. Systemic infection was cauded in Habenaria radiata, and local infection was produced in Chenopodium amaranticolor, C. quinoa, Spinacia olracea, Tetragonia expansa and Gomphrena globosa. HaMV was also transmitted by Myzus persicae in a non persistent manner, but not by Aphis gossypii. The virus has filamentous particles of c. 750×13nm. Sap from inoculated C. quinoa was infective after dilution to 10-5 but not 10-6, after 10 min at 60℃ but 65℃, or after 8-16 days at 20℃.In ultrathin sections of diseased leaves of Habenaria radiata and inoculated C. quinoa, virus particles were found to disperse in the cytoplasm. Cylindrical inclusions were present in the cytoplasm of infected cells, as pinwheels, scrolls or tubes. The inclusions observed in negatively stained crude sap preparations exhibited fine linear striation at about 5.2 nm intervals.The antiserum to HaMV produced by immunizing a rabbit had a titer of 1/512(precipitin test). HaMV showed distant serological relationships to potyvirus;alstroemeria mosaic virus, bean yellow mosaic virus, calanthe mild mosaic virus, clover yellow vein virus, dendrobium mosaic virus, freesia mosaic virus, turnip mosaic virus and watermelon mosaic virus 2. |
| キーワード | Habenaria orchid Habenaria mosaic potyvirus Habenaria mosaic disease |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 155 |
| 終了ページ | 168 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 |
|---|---|
| フルテキストURL | 005_002_145_153.pdf |
| 著者 | 杉本 学| 河合 富佐子| |
| 抄録 | A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. |
| キーワード | Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 145 |
| 終了ページ | 153 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
|---|---|
| フルテキストURL | 005_002_135_144.pdf |
| 著者 | 今野 晴義| 積木 久明| 山崎 良樹| |
| 抄録 | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| キーワード | Cell wall Oryza sativa Pectic polymer |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 135 |
| 終了ページ | 144 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Purification and Properties of α-Glucodidase from Taro Tuber |
|---|---|
| フルテキストURL | 005_002_129_134.pdf |
| 著者 | 間島 英之| 山崎 良樹| 今野 晴義| |
| 抄録 | α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. |
| キーワード | α-glucosidase taro tuber Colocasia esculanta Shott |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 129 |
| 終了ページ | 134 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ナメクジのα-グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 005_002_121_127.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| キーワード | Slug Incilaria bilineata α-glucosidase |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 121 |
| 終了ページ | 127 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |