検索結果 118 件
| フルテキストURL | fulltext20220413-6-2.pdf |
|---|---|
| 著者 | Sato, Yukiyo| Shahi, Sabitree| Telengech, Paul| Hisano, Sakae| Cornejo, Carolina| Rigling, Daniel| Kondo, Hideki| Suzuki, Nobuhiro| |
| キーワード | Splipalmivirus Capsidless RNA virus Cryphonectria naterciae Narnavirus Fungal virus Mycovirus |
| 備考 | © 2021 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0 License. http://creativecommons.org/licenses/by-nc-nd/4.0/. This is the accepted manuscript version. The formal published version is available at [https://doi.org/10.1016/j.virusres.2021.198606] .| |
| 発行日 | 2022-01 |
| 出版物タイトル | Virus Research |
| 巻 | 307巻 |
| 号 | 2号 |
| 出版者 | Elsevier BV |
| 開始ページ | 198606 |
| ISSN | 0168-1702 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 Elsevier B.V. |
| 論文のバージョン | author |
| PubMed ID | 34688782 |
| DOI | 10.1016/j.virusres.2021.198606 |
| Web of Science KeyUT | 000767885500002 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.virusres.2021.198606 |
| フルテキストURL | fulltext20220413-6-1.pdf |
|---|---|
| 著者 | Telengech, Paul| Shahi, Sabitree| Kondo, Hideki| Suzuki, Nobuhiro| |
| 備考 | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00705-022-05372-3| This fulltext is available in March 2023.| |
| 発行日 | 2022-3-4 |
| 出版物タイトル | Archives of Virology |
| 巻 | 167巻 |
| 号 | 4号 |
| 出版者 | Springer Science and Business Media LLC |
| 開始ページ | 1201 |
| 終了ページ | 1204 |
| ISSN | 0304-8608 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022 |
| 論文のバージョン | author |
| PubMed ID | 35246731 |
| DOI | 10.1007/s00705-022-05372-3 |
| Web of Science KeyUT | 000764476800001 |
| 関連URL | isVersionOf https://doi.org/10.1007/s00705-022-05372-3 |
| JaLCDOI | 10.18926/AMO/62807 |
|---|---|
| フルテキストURL | 75_6_685.pdf |
| 著者 | Yamashita, Mampei| Kuroki, Tamotsu| Hamada, Takashi| Hirayama, Takanori| Tokunaga, Takayuki| Yamanouchi, Kosho| Takeshita, Hiroaki| Maeda, Shigeto| |
| 抄録 | Magnetic resonance cholangiopancreatography (MRCP) is a non-invasive imaging technique that provides high-quality visualization of the biliary tree, including the gallbladder. This study aimed to evaluate the useful-ness of preoperative MRCP for acute cholecystitis in predicting technical difficulties during laparoscopic chole-cystectomy (LC). A total of 168 patients who underwent LC with preoperative MRCP were enrolled in this study. Patients were divided into two groups according to preoperative MRCP findings: the visualized group (n = 126), in which the entire gallbladder could be visualized; and the non-visualized group (n = 42), in which the entire gallbladder could not be visualized. The perioperative characteristics and postoperative complica-tions of the two groups were retrospectively analyzed. Operation time was longer in the non-visualized group (median 101.5 vs. 143.5 min; p < 0.001). The non-visualized group had significantly more intraoperative blood loss than the visualized group (median 5 vs. 10 g; p = 0.05). The rate of conversion to open cholecystectomy was significantly higher in the non-visualized group (1.6 vs. 9.5%; p = 0.03). In conclusion, patients in the non- visualized group showed higher difficulty in performance of LC. Our MRCP-based classification is a simple and effective means of predicting difficulties in performing LC for acute cholecystitis. |
| キーワード | laparoscopic cholecystectomy magnetic resonance cholangiopancreatography acute cholecystitis gallbladder disease non-invasive imaging |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2021-12 |
| 巻 | 75巻 |
| 号 | 6号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 685 |
| 終了ページ | 689 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | Copyright Ⓒ 2021 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 34955535 |
| Web of Science KeyUT | 000735297900003 |
| NAID | 120007180285 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Kondo, Hideki| Yoshida, Naoto| Fujita, Miki| Maruyama, Kazuyuki| Hyodo, Kiwamu| Hisano, Hiroshi| Tamada, Tetsuo| Andika, Ida Bagus| Suzuki, Nobuhiro| |
| キーワード | Betaflexiviridae quinvirus bymovirus yellow mosaic disease wheat virome soil borne variants |
| 発行日 | 2021-08-19 |
| 出版物タイトル | Frontiers In Microbiology |
| 巻 | 12巻 |
| 出版者 | Frontiers Media SA |
| 開始ページ | 715545 |
| ISSN | 1664-302X |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 Kondo, Yoshida, Fujita, Maruyama, Hyodo, Hisano, Tamada, Andika and Suzuki. |
| 論文のバージョン | publisher |
| PubMed ID | 34489904 |
| DOI | 10.3389/fmicb.2021.715545 |
| Web of Science KeyUT | 000692554100001 |
| 関連URL | isVersionOf https://doi.org/10.3389/fmicb.2021.715545 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Mori, Tomoaki| Warner, Chiaki| Ohno, Serika| Mori, Koichi| Tobimatsu, Takamasa| Sera, Takashi| |
| キーワード | Plum pox virus Complete genome sequence Phylogenetic analysis Sequence alignment analysis Genetic variation |
| 発行日 | 2021-07-10 |
| 出版物タイトル | BMC Research Notes |
| 巻 | 14巻 |
| 号 | 1号 |
| 出版者 | Springer Nature |
| 開始ページ | 266 |
| ISSN | 1756-0500 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © The Author(s) 2021. |
| 論文のバージョン | publisher |
| PubMed ID | 34246294 |
| DOI | 10.1186/s13104-021-05683-9 |
| Web of Science KeyUT | 000672644300002 |
| 関連URL | isVersionOf https://doi.org/10.1186/s13104-021-05683-9 |
| 著者 | Sakamoto, Keiji| Tomonari, Misaki| Ariya, Uyanga| Nakagiri, Erika| Matsumoto, Tetsuya| Akaji, Yasuaki| Otoda, Takashi| Hirobe, Muneto| Nachin, Baatarbileg| |
|---|---|
| キーワード | Disturbance Forest floor Salvage logging Seed source Succession |
| 備考 | metadata only access. The Version of Record is available online at: http://dx.doi.org/10.1007/s11355-021-00457-8| |
| 発行日 | 2021-04-17 |
| 出版物タイトル | Landscape and Ecological Engineering |
| 巻 | 17巻 |
| 出版者 | Springer Science and Business Media LLC |
| 開始ページ | 267 |
| 終了ページ | 279 |
| ISSN | 1860-1871 |
| NCID | AA12090017 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | none |
| DOI | 10.1007/s11355-021-00457-8 |
| Web of Science KeyUT | 000640827800001 |
| 関連URL | isVersionOf https://doi.org/10.1007/s11355-021-00457-8 |
| フルテキストURL | fulltext20210525_1.pdf |
|---|---|
| 著者 | Laukhtina, Ekaterina| Abufaraj, Mohammad| Al-Ani, Abdallah| Ali, Mustafa Rami| Mori, Keiichiro| Moschini, Marco| Quhal, Fahad| Sari Motlagh, Reza| Pradere, Benjamin| Schuettfort, Victor M.| Mostafaei, Hadi| Katayama, Satoshi| Grossmann, Nico C.| Fajkovic, Harun| Soria, Francesco| Enikeev, Dmitry| Shariat, Shahrokh F.| |
| キーワード | Non–muscle-invasive bladder cancer Bladder cancer Intermediate risk Intravesical therapy Network meta-analysis |
| 発行日 | 2021-03-21 |
| 出版物タイトル | European Urology Focus |
| 出版者 | Elsevier |
| ISSN | 24054569 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2021 The Authors. |
| 論文のバージョン | publisher |
| PubMed ID | 33762203 |
| DOI | 10.1016/j.euf.2021.03.016 |
| NAID | 120007039929 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.euf.2021.03.016 |
| フルテキストURL | Nat_Microbiol_15001.pdf |
|---|---|
| 著者 | Zhang, Rui| Hisano, Sakae| Tani, Akio| Kondo, Hideki| Kanematsu, Satoko| Suzuki, Nobuhiro| |
| キーワード | Molecular evolution Viral genetics |
| 発行日 | 2016-01-11 |
| 出版物タイトル | Nature Microbiology |
| 巻 | 1巻 |
| 号 | 1号 |
| 出版者 | Nature Research |
| 開始ページ | 15001 |
| ISSN | 2058-5276 |
| NCID | AA1278421X |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| PubMed ID | 27571749 |
| DOI | 10.1038/NMICROBIOL.2015.1 |
| Web of Science KeyUT | 000381577400001 |
| 関連URL | isVersionOf https://doi.org/10.1038/nmicrobiol.2015.1 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Masuda, Kanae| Yamamoto, Eiji| Shirasawa, Kenta| Onoue, Noriyuki| Kono, Atsushi| Ushijima, Koichiro| Kubo, Yasutaka| Tao, Ryutaro| Henry, Isabelle M.| Akagi, Takashi| |
| キーワード | flexible sexuality monoecy polyploid GWAS |
| 発行日 | 2020-06-17 |
| 出版物タイトル | DNA Research |
| 巻 | 27巻 |
| 号 | 3号 |
| 出版者 | OXFORD UNIV PRESS |
| 開始ページ | dsaa012 |
| ISSN | 1340-2838 |
| NCID | AA11095219 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © The Author(s) 2020. |
| 論文のバージョン | publisher |
| PubMed ID | 32761076 |
| DOI | 10.1093/dnares/dsaa012 |
| Web of Science KeyUT | 000577169400006 |
| 関連URL | isVersionOf https://doi.org/10.1093/dnares/dsaa012 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Mitalo, Oscar W.| Otsuki, Takumi| Okada, Rui| Obitsu, Saeka| Masuda, Kanae| Hojo, Yuko| Matsuura, Takakazu| Mori, Izumi C.| Abe, Daigo| Asiche, William O.| Akagi, Takashi| Kubo, Yasutaka| Ushijima, Koichiro| |
| キーワード | 1-methylcyclopropene carotenoids chlorophyll Citrus limon ethylene low temperature peel degreening phytohormones transcriptome |
| 発行日 | 2020-05-06 |
| 出版物タイトル | Journal of Experimental Botany |
| 巻 | 71巻 |
| 号 | 16号 |
| 出版者 | Oxford University Press |
| 開始ページ | 4778 |
| 終了ページ | 4796 |
| ISSN | 0022-0957 |
| NCID | AA00697526 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © The Author(s) 2020. |
| 論文のバージョン | publisher |
| PubMed ID | 32374848 |
| DOI | 10.1093/jxb/eraa206 |
| Web of Science KeyUT | 000573250000015 |
| 関連URL | isVersionOf https://doi.org/10.1093/jxb/eraa206 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Miyata, Makoto| Robinson, Robert C.| Uyeda, Taro Q. P.| Fukumori, Yoshihiro| Fukushima, Shun‐ichi| Haruta, Shin| Homma, Michio| Inaba, Kazuo| Ito, Masahiro| Kaito, Chikara| Kato, Kentaro| Kenri, Tsuyoshi| Kinosita, Yoshiaki| Kojima, Seiji| Minamino, Tohru| Mori, Hiroyuki| Nakamura, Shuichi| Nakane, Daisuke| Nakayama, Koji| Nishiyama, Masayoshi| Shibata, Satoshi| Shimabukuro, Katsuya| Tamakoshi, Masatada| Taoka, Azuma| Tashiro, Yosuke| Tulum, Isil| Wada, Hirofumi| Wakabayashi, Ken‐ichi| |
| キーワード | appendage cytoskeleton flagella membrane remodeling Mollicutes motor protein peptidoglycan three domains |
| 発行日 | 2020-01-19 |
| 出版物タイトル | Genes to Cells |
| 巻 | 25巻 |
| 号 | 1号 |
| 出版者 | Wiley |
| 開始ページ | 6 |
| 終了ページ | 21 |
| ISSN | 1356-9597 |
| NCID | AA11078945 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2020 The Authors. |
| 論文のバージョン | publisher |
| PubMed ID | 31957229 |
| DOI | 10.1111/gtc.12737 |
| Web of Science KeyUT | 000507856400001 |
| 関連URL | isVersionOf https://doi.org/10.1111/gtc.12737 |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Telengech, Paul| Hisano, Sakae| Mugambi, Cyrus| Hyodo, Kiwamu| Arjona-Lopez, Juan Manuel| Lopez-Herrera, Carlos Jose| Kanematsu, Satoko| Kondo, Hideki| Suzuki, Nobuhiro| |
| キーワード | partitivirus dsRNA virus phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica diversity reassortment horizontal transfer |
| 発行日 | 2020-06-26 |
| 出版物タイトル | Frontiers in Microbiology |
| 巻 | 11巻 |
| 出版者 | Frontiers Media |
| 開始ページ | 1064 |
| ISSN | 1664-302X |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2020 Telengech et al. |
| 論文のバージョン | publisher |
| PubMed ID | 32670213 |
| DOI | 10.3389/fmicb.2020.01064 |
| Web of Science KeyUT | 000551691300001 |
| 関連URL | isVersionOf https://doi.org/10.3389/fmicb.2020.01064 |
| フルテキストURL | fulltext.pdf Tables.pdf Figures.pdf |
|---|---|
| 著者 | Hirata, Seiji| Hayashi, Saori| Ohta, Masamitsu| |
| キーワード | Three sections of wood Glossiness Color measurement L*a*b* color space L*C*h color space Density Average of annual ring width |
| 備考 | This is a post-peer-review, pre-copyedit version of an article published in Journal of Wood Science. The final authenticated version is available online at: https://doi.org/10.1186/s10086-020-01883-2.| |
| 発行日 | 2020-05-19 |
| 出版物タイトル | Journal of Wood Science |
| 巻 | 66巻 |
| 出版者 | Springer |
| ISSN | 1435-0211 |
| NCID | AA11200007 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| DOI | 10.1186/s10086-020-01883-2 |
| Web of Science KeyUT | 000533783500001 |
| 関連URL | isVersionOf https://doi.org/10.1186/s10086-020-01883-2 |
| フルテキストURL | CH38_1_14.pdf |
|---|---|
| 著者 | Tanabe, Shintaro| Kasuya, Eiiti| Miyatake, Takahisa| |
| キーワード | Habituation Hyla japonica individual variation sexual difference predation |
| 発行日 | 2019-02-28 |
| 出版物タイトル | Current Herpetology |
| 巻 | 38巻 |
| 号 | 1号 |
| 出版者 | Herpetological Society of Japan |
| 開始ページ | 14 |
| 終了ページ | 22 |
| ISSN | 1345-5834 |
| NCID | AA12410796 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 論文のバージョン | author |
| DOI | 10.5358/hsj.38.14 |
| Web of Science KeyUT | 000483742900003 |
| 関連URL | isVersionOf https://doi.org/10.5358/hsj.38.14 |
| タイトル（別表記） | Sexuality in plants, unveiled from genome evolution |
|---|---|
| フルテキストURL | srfa_109_037_040.pdf |
| 著者 | 赤木 剛士| |
| 抄録 | Sexual polymorphism, a main strategy to maintain genetic diversity within a species, has long been a major focus in biology. Notwithstanding, in plants, evolution of sexual systems and mechanisms underlying these transitions have been little unveiled. We have elucidated the molecular mechanism of sex determination in persimmons (Diospyros spp.), where the Y-encoded smRNA gene OGI can repress the female-determining gene MeGI, and also in kiwifruits (Actinidia spp.), where the Y-encoded two sex determinants, Shy Girl and Friendly Boy, control gynoecium and androecium development, respectively. Although the molecular functions of these determinants are distinct, they have common evolutionary scenarios involving transitions of sexual systems. In persimmon, a recent genome triplication (hexaploidization) in cultivated persimmon (D. kaki) derived “flexible” sexuality via establishing epigenetic layers on the two sex determinants. On the other hand, an ancient Diospyros-specific paleo-genome duplication (paleo-tetraploidization) enabled neofunctionalization in the proto-MeGI, via positive selection, to establish a new function as a sex determinant. In kiwifruit, one of the two sex determinants, Shy Girl, was derived from neofunctionalization via Actinidia-specific duplication event. These findings exemplify how plant-specific numerous duplication events can drive flexible genetic material whose variation can be selected for development of new sexual systems. |
| キーワード | Sex determination Sex chromosome Polyploidization Genome evolution Tree crops |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 発行日 | 2020-02-01 |
| 巻 | 109巻 |
| 開始ページ | 37 |
| 終了ページ | 40 |
| ISSN | 2186-7755 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | fulltext.pdf |
|---|---|
| 著者 | Nakamura, Shunsuke| Yamada, Taihei| Nojima, Tsuyoshi| Naitou, Hiromichi| Koga, Hitoshi| Yamashita, Hisashi| Gochi, Akira| Nakao, Atsunori| |
| キーワード | Acute care surgery Computed tomography Mesenteric hematoma |
| 発行日 | 2019-12-31 |
| 出版物タイトル | International Journal of Surgery Case Reports |
| 巻 | 65巻 |
| 出版者 | Elsevier |
| 開始ページ | 124 |
| 終了ページ | 126 |
| ISSN | 22102612 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| OAI-PMH Set | 岡山大学 |
| 著作権者 | © 2019 The Author(s). Published by Elsevier Ltd on behalf of IJS Publishing Group Ltd. |
| 論文のバージョン | publisher |
| PubMed ID | 31715445 |
| DOI | 10.1016/j.ijscr.2019.10.058 |
| Web of Science KeyUT | 000501862100027 |
| 関連URL | isVersionOf https://doi.org/10.1016/j.ijscr.2019.10.058 |
| タイトル（別表記） | Species identification of a laboratory strain belonging to the family Arvicolinae |
|---|---|
| フルテキストURL | poalas_035_012_017.pdf |
| 著者 | 高畑 智| 伊勢村 夏実| 目加田 和之| |
| 抄録 | This study investigated the taxonomic status of a laboratory strain belonging to the family Arvicolinae for which the wild species name is unknown. This vole strain, though considered formerly a Lemming, was distinguishable from any Lemmus species by features of its appearance, skull and molar morphology, and conventional chromosomal pattern. Subsequently, we read the nucleotide sequence of the mitochondrial cytochrome b (Cyb) gene and constructed a molecular phylogenetic tree. We found that this vole strain belongs to the same clade as Microtus guenthe |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2019-06 |
| 巻 | 35巻 |
| 開始ページ | 12 |
| 終了ページ | 17 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | K0005983_abstract_review.pdf K0005983_summary.pdf |
|---|---|
| 著者 | DINH TIEN TAI| |
| 発行日 | 2019-03-25 |
| 資料タイプ | 学位論文 |
| 学位授与番号 | 甲第5983号 |
| 学位授与年月日 | 2019-03-25 |
| 学位・専攻分野 | 博士（学術） |
| 授与大学 | 岡山大学 |
| 言語 | 英語 |
| JaLCDOI | 10.18926/AMO/55665 |
|---|---|
| フルテキストURL | 72_1_67.pdf |
| 著者 | Watanabe, Akihisa| Ono, Qana| Nishigami, Tomohiko| Hirooka, Takahiko| Machida, Hirohisa| |
| 抄録 | It has been unclear whether the risk factors for rotator cuff tears are the same at all ages or differ between young and older populations. In this study, we examined the risk factors for rotator cuff tears using classification and regression tree analysis as methods of nonlinear regression analysis. There were 65 patients in the rotator cuff tears group and 45 patients in the intact rotator cuff group. Classification and regression tree analysis was performed to predict rotator cuff tears. The target factor was rotator cuff tears; explanatory variables were age, sex, trauma, and critical shoulder angle≥35°. In the results of classification and regression tree analysis, the tree was divided at age 64. For patients aged≥64, the tree was divided at trauma. For patients aged<64, the tree was divided at critical shoulder angle≥35°. The odds ratio for critical shoulder angle≥35° was significant for all ages (5.89), and for patients aged<64 (10.3) while trauma was only a significant factor for patients aged≥64 (5.13). Age, trauma, and critical shoulder angle≥35° were related to rotator cuff tears in this study. However, these risk factors showed different trends according to age group, not a linear relationship. |
| キーワード | rotator cuff tears risk factor critical shoulder angle trauma classification and regression tree analysis |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2018-02 |
| 巻 | 72巻 |
| 号 | 1号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 67 |
| 終了ページ | 72 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2018 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 29463941 |
| タイトル（別表記） | Virus-induced gene silencing in Prunus fruit tree species with the Apple latent spherical virus vector |
|---|---|
| フルテキストURL | srfa_107_011_017.pdf |
| 著者 | 河井 崇| |
| 抄録 | Virus-induced gene silencing (VIGS) has been used as a rapid and effective tool for functional analysis of genes in various plants, including woody fruit tree species. In this study, we attempted to develop a VIGS-based gene evaluation system for seven Prunus species, including apricot (P. armeniaca L.), sweet cherry (P. avium L.), almond [P. dulcis (Mill.) D. A. Webb.], peach (P. persica Batsch), Japanese apricot (P. mume Siebold & Zucc.), Japanese plum (P. salicina Lindl.), and European plum (P. domestica L.), with the Apple latent spherical virus (ALSV) vectors. ALSV vectors carrying part of the apricot PHYTOENE DESATURASE (PDS) gene sequence were amplified in Nicotiana benthamiana, and inoculated into the cotyledons of Prunus seedlings by particle bombardment. Typical PDS-silenced phenotypes, characterized by uniform discoloration of the upper leaves, were observed in sweet cherry and some cultivars of apricot and almond several weeks after inoculation. In contrast, attempted ALSV infections of Japanese apricot, Japanese plum, European plum, and the other cultivars of apricot and almond were unsuccessful. Furthermore, although the infection rate of ALSV in peach was high, severe viral infection symptoms were observed in the infected leaves. These results collectively suggested that the efficiency of ALSV infection and VIGS could vary depending on species and/or cultivar in Prunus. The possible use of the ALSV-mediated VIGS system for functional analysis of genes in Prunus is discussed. |
| キーワード | gene evaluation system post-transcriptional gene silencing virus vector |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 発行日 | 2018-02-01 |
| 巻 | 107巻 |
| 開始ページ | 11 |
| 終了ページ | 17 |
| ISSN | 2186-7755 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |