検索結果 20766 件
| タイトル（別表記） | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
|---|---|
| フルテキストURL | 005_002_135_144.pdf |
| 著者 | 今野 晴義| 積木 久明| 山崎 良樹| |
| 抄録 | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| キーワード | Cell wall Oryza sativa Pectic polymer |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 135 |
| 終了ページ | 144 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Secretion of Defensive Substance by Carabidae and Brachinidae |
|---|---|
| フルテキストURL | 004_001_009_023.pdf |
| 著者 | 兼久 勝夫| |
| 抄録 | The secretion of defensive substances by more than 250 species of carabid and brachinid beetles was investigated, and discussed from a phylogenetical viewpoint. The morphological of secretion glands and secreted components were identified in the genus level. Specific subgenus and species had peculiar characteristics. The pair of defensive organs consisted of many small synthetic lobes, large reservoirs, collecting ducts from lobes to reservoirs and secretion ducts from reservoirs. The pair dust opened at the last sternal intersegmental membrane. There was species specificity in the reservoir shape, synthetic lobe shape and entering site of the collecting ducts into reservoirs. The spherical shape lobes produced short chain fatty acids, the elongated shape lobes produced m-cresol and the thick board shape lobes produced benzoquinones. Two types of fatty acid-secreting beetles were found, one secreted formic acid, and the other mixed short chain fatty acids. These short chain fatty acids were metabolized from some amino acids, methacrylic acid was from valine; tiglic acid, α-ethyacrylic acid and angelic acid were from isoleucine; senecioic acid was from leucine and crotonic acid was from lysine. These unasaturated acids were sometimes detected with a trace amount of corresponding saturated acids. A few species secreted larger amounts of saturated acids with a trace amounts of unsaturated acids. Fatty acid composition was generally genus-specific. In a few cases, however, species-specific acid composition was also observed. m-Cresol-secreting beetles were observed only in Panagaeni and Chlaeniini except subgenus Chlaenius Chlaeniini except subgenus Chlaenius Chlaeniellus beetle. 1,4-Benzoquinone and 2-methyl-1,4-benzoquinone-secreting beetles were observed in subgenus C. Chlaeniellus. This beetle secreted these substances at body temperature from a reservoir with no sound. Brachinidae beetles, called bombardier beetles, have a pair of brownish reaction chamber connected with a reservoir. They sprayed the two benzoquinones at about 100℃ making sound. |
| キーワード | Defensive secreting substance Synthetic organ Carabidae Brachinidae Phylogenetic relation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 9 |
| 終了ページ | 23 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ナメクジのα-グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 005_002_121_127.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| キーワード | Slug Incilaria bilineata α-glucosidase |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 121 |
| 終了ページ | 127 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Varietal Variation and Effects of Some Major Genes on Salt Tolerance in Barley Seedlins |
|---|---|
| フルテキストURL | 003_001_071_081.pdf |
| 著者 | 間野 吉郎| 武田 和義| |
| 抄録 | In order to select the salt tolerant cultivars, and to evaluate the effects of some major genes on salt tolerance in barley, 5,182 varieties and 368 isogenic lines were tested by exposing to 1.0 and 0.5M sodium chloride at the seedling stage. The average rating of salt tolerance was higher in varieties from Korea and Japan , but lower in varieties from Nepal and Southwest Asia. When the varieties were grouped by several morphological characters, six-rowed, covered, E type of nonbrittle rachis and uzu type groups were more tolerant than two-rowed, naked, W type and non-uzu type counterparts, respectively. A comparison between the isogenic pairs revealed that the uzu type was more tolerant than non-uzu type, but other major gene pairs did not show any obvious differences. These findings indicate that the uz gene affects the salt tolerance, and diferences between varietal groups characterized by other major genes are due to the difference in the genetic background among these varieties. |
| キーワード | Barley Seedling Salt tolerance Screening Isogenic line |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 71 |
| 終了ページ | 81 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Diallel Analysis of 1,000-grain Weight in Two-rowed Barley Varieties |
|---|---|
| フルテキストURL | 003_001_063_070.pdf |
| 著者 | 呉 基日| 武田 和義| |
| 抄録 | The inheritance of 1,000-grain weight of two-rowed barley varieties was examined using a reciprocal F1 cross and a half F2 diallel cross among eight parents. The F1 generation was examined in five different ripening conditions i.e., (Ⅰ) control, (Ⅱ) leaf cut, in which all leaves were cut at the heading time, (Ⅲ) Spikelet thinned, in which a quarter of the spikelets were thinned at the heading time, (Ⅳ) dry, in which the plants were grown in a vinyl-film house without irrigation, and (Ⅴ) wet, in which the plants were mist-irrigated every two house in the daytime after the heading to the harvest. The experiment was repeated twice. The F2 populations were grown in the ordinary condition. The average 1,000-grain weight was largest in the dry plot followed by spikelet thinned, control, leaf cut, and wet plots. The 1,000-grain weight was predominantly controlled by the additive genes. The dominance effect was also significant. The large grain weight was controlled by the overdominant genes. Epistasis among the genes controlling the 1,000-grain weight was significant, but the maternal effect was absent. In the F1 diallel, heritability was 0.3~0.6 and 0.2~0.5 in a broad and a narrow sense, respectively. In the F2 diallel, it was 0.9 and 0.6 in a broad and a narrow sense, respectively. In each of the 28 F2 populations, the broad sense heritability was estimated as 0.3~0.8. |
| キーワード | Barley Grain size Diallel analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 63 |
| 終了ページ | 70 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Production of Strain of Erysiphe graminis f. sp. tritici That Are Pathogenic on Barley and Agropyron |
|---|---|
| フルテキストURL | 004_002_229_237.pdf |
| 著者 | 部田 英雄| 井上 成信| |
| 抄録 | Two isolates of Erysiphe graminis f. sp. tritici, T3 and T4, were crossed and selected for pathogenicity. The corssing and selection process were repeated five times with the progenies. Isolates of each generation were inoculated on the non-hosts, Hordeum vulgare and Agropyron tsukushiense var. transiens OHWI, and parasitism and pathogenicity were investigated with respect to host specificity.As shown in Tables 2 to 5, the process of crossing and selection produced isolates which were pathogenic on barley cultivars, 'Kagoshimahadaka' or 'Russian 9' or Agropyron tsukushiense ecotypes 'Agropyron Early ecotype' or 'Agropyron Yezoense 3'.Factors affecting the degree of pathogenicity appear to be under polygenic control. The cumulative effect of minor genes may play a role in the differentiation of formae speciales suggesting a new concept of pathogenicity in the powdery mildew of grasses. |
| キーワード | Wheat powdery mildew Host specific Pathogenicity Properties Barley Agropyron |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 229 |
| 終了ページ | 237 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Comparison between shoot Regeneration Ability in Calli Derived from Immature Embryos of Wild and Cultivated Barley |
|---|---|
| フルテキストURL | 003_001_055_062.pdf |
| 著者 | 力石 和英| 武田 和義| 安田 昭三| |
| 抄録 | The variation in shoot regeneration obility of calli derived from immature embryos was examined in 95 wild strains, 82 of which were of Hordeum spontaneum and 13 of which were H. agriocrithon, and 87 cultivated varieties collected from various countries or regions of the world. In 85 strains of the wild species, a number of calli regenerated shoots, and their proportion ranged from 1.2% to 75.7%. The average percentage of shoot regenerating calli was 21.7% among the strains that formed calli, 11.5% of which regenerated green and 10.2% albino shoots. On average, 21.4% and 23.9% calli regenerated shoots in H. spontaneum and H. agriocrithon, respectively and there was no significant difference between these values. A significant difference in the percentage of shoot regenerating calli was found among six variants (dawense, ishnatherum, laguncliforme, paradoxon, proskowetzii, spontaneum) which were comprised in H. spontaneum. In 73 varieties of cultivated species, there were shoots regenerating calli likely to wild species, and their proportion ranged from 3.2% to 85.5%. The average percentage of shoot regenerating calli was 25.4%, 22.0% of which regenerated green and 3.4% of which regenerated albino shoots. There was a significant difference in percentage of green shoots regenerating calli against shoots regenerating ones between the wild (53.0%) and cultivated species 886.6%). The two kinds of non-brittle rachis genotypes, Bt bt2 and bt Bt2 are one of the key characters distinguishing the oriental and occidental types of cultivated barley. The average percentages of shoot regenerating calli were 16.2% and 32.3% for the genotypes Bt bt2 and bt Bt2, respectively, suggesting that there is a geographical variation in the shoot regeneration ability of calli in the cultivated species. By contrast, the oriental and occidental strains of wild species showed no difference in the shoot regeneration ability of calli. The geographical variation of shoot regeneration ability differed significantly between wild and cultivated species. This suggests that the geographical variation of shoot regeneration ability occurred after the cultivation of the barley was established. |
| キーワード | Hordeum spontaneum Hordeum agriocrithon Tissue culture Geographical variation Shoot regeneration ability |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 55 |
| 終了ページ | 62 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Some Properties of Azuki Bean Mosaic Virus Isolated from Soybean Plants in Ibaraki Prefecture |
|---|---|
| フルテキストURL | 004_002_215_227.pdf |
| 著者 | 高橋 幸吉| 井上 成信| 前田 孚憲| 光畑 興二| 千田 茂樹| 高橋 義行| |
| 抄録 | Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40～50% less than that of healthy plants. |
| キーワード | Potyvirus Azuki bean mosaic virus Glycine max Properties |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 215 |
| 終了ページ | 227 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 大麦網斑病抵抗性の選抜効果 |
|---|---|
| フルテキストURL | 003_001_043_053.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. |
| キーワード | Net blotch Pyrenophora teres Selection Barley Disease resistance |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 43 |
| 終了ページ | 53 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120004537480 |
| タイトル（別表記） | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
|---|---|
| フルテキストURL | 003_001_035_041.pdf |
| 著者 | 侯 福分| 曽 富生| 呉 詩都| 武田 和義| |
| 抄録 | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| キーワード | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 35 |
| 終了ページ | 41 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Ixia から分離された bean yellow mosaic virus |
|---|---|
| フルテキストURL | 004_002_201_213.pdf |
| 著者 | 辻 俊也| 前田 孚憲| 近藤 秀樹| 井上 成信| |
| 抄録 | A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. |
| キーワード | Ixia hybrida Bean yellow mosaic virus Potyvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 201 |
| 終了ページ | 213 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313395 |
| タイトル（別表記） | Sorghum Resistance to the Sugarcane Aphid, Melanaphis sacchari (Zehntenr) Amounts of Surface Wax and Nutritional Components |
|---|---|
| フルテキストURL | 003_001_027_034.pdf |
| 著者 | 積木 久明| 兼久 勝夫| Moharramipour Saeid| |
| 抄録 | The differences in the amounts of leaf surface wax and nutritional components such as sugar and free amino acids were compared among resistant (PE 954177), moderately resistant (IS 84) and susceptible (Redlan B) sorghum varieties to the sugarcane aphid. Melanaphis sacchari (Zehntner). (1) The kind of aphid appearing on the leaves of the sorghum varieties was only the sugarcane aphid, Melanaphis sacchari in the field. The number of aphids infesting a leaf was ca 0,30 and 3,500 in the resistant, moderately resistant and susceptible varieties at the middle of August, respectively. (2) The amount of leaf surface wax was approximately constant among these three varieties. (3) The total sugar content was slightly higher in the two resistant varieties than in the susceptible variety. (4) The total free amino acid concentration was higher in the two resistant varieties than in the susceptible variety. (5) These findings suggest that neither leaf surface wax nor nutritional components seem to affect the aphid populations on the leaves of the three sorghum varieties, showing that biogenic substances such as dhurrin, benzylalcohol, p-hydroxybenzaldehyde, aconitic acide, etc. may affect the aphid populations. |
| キーワード | Sorghum Sugarcane aphid Melanaphis sacchari Leaf surface wax Amino acid |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 27 |
| 終了ページ | 34 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Some Properties of Cymbidium Mosaic Virus Isolated from Calanthe spp. |
|---|---|
| フルテキストURL | 004_002_187_199.pdf |
| 著者 | 松本 純一| 占部 慎治| 前田 孚憲| 光畑 興二| 近藤 秀樹| 田原 望武| 井上 成信| |
| 抄録 | Cymbidium mosaic virus(CyMV) was isolated from Calanthe spp. showing mosaic on the leaves, collected in Yamaguchi and Kyoto Prefectures in 1986～1993. CyMV, Cal. 90-1 isolate was transmitted by sapinoculation to 12 out of 37 species in 7 out of 9 families. Sap from diseaded Tetragonia expansa was infective to Chenopodium amaranticolor after dilution to 10-5 but not 10-6, after heating at 65℃ for 10 min but not 70℃, and after 1 month at 20℃ but not 2 months. The virus particles were flexuous rod, about 475 nm long. The virus was purified from diseased T. expansa leaves and contained a single protein species of Mr27,800. The Mr of the capsid proteins(Cal. 90-1) was similar to those of two ohter CyMV isolates(Cal. 90-4, Cal. 93-14).Cal. 90-1 and Cal. 93-14 reacted with antiserum to the Cymbidium isolate (Cy-16), suggesting that Cal. 90-1 was serologically very similar to the other two CyMV isolates. Two species of dsRNA were isolated from plants infected with Cal-1 and they were similar to those of two other CyMv isolates. |
| キーワード | Calanthe spp. Cymbidium mosaic virus Potexvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 187 |
| 終了ページ | 199 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313956 |
| タイトル（別表記） | Quantitative Variations of a Resistance Substance, DIMBOA, against Aphids in Wheat Varieties. |
|---|---|
| フルテキストURL | 003_001_017_026.pdf |
| 著者 | 兼久 勝夫| Awan Rustamani Maqsood| 鄭 文儀| 積木 久明| 白神 孝| |
| 抄録 | Aphids sometimes severely infest wheat plants, mainly sucking phloem sap and disrupting tissues, and in a few cases act as virus vectors. There are resistant and susceptible varieties of wheat against aphids. DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) is a substance causing resistance to animals including aphids. The change in the amounts of DIMBOA with growth in eight wheat varieties was estimated in 1990 and 1991. Wheat seeds were sown at mid-November in the former year and harvested in mid-June. Aphids appeared from early April, increased with the growth of the wheat, and finally decreased with the senescence of the wheat. Rhopalosiphum maidis appeared early in the season, R. padi appeared late, Schizaphis graminum and Sitovion akebiae appeared intermittently in the season. S. graminum appeared more frequently on wheat than barley. DIMBOA was detected from wheat but not from barley. Gramine (N,N-dimethyl-indole-3-methanamine) was detected from barley, and is known as an important resistance substance. However gramine could not be detected in wheat. DIMBOA was found in higher amounts in young wheat, and gradually decreased with growth. A clear relationship between the aphid population and DIMBOA amounts could not be observed. However, all the wheat varieties used in this experiment seemed to have resistance against aphids. The resistance was compared with barley susceptible lines. DIMBOA was presumed to share the property of resistance with aconitic acid in wheat. |
| キーワード | DIMBOA content Wheat Aphid Resistant varaity Susceptible variety |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 17 |
| 終了ページ | 26 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
|---|---|
| フルテキストURL | 004_002_164_174.pdf |
| 著者 | Gara I Wayan| 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| キーワード | Vanda orchid Cymbidium mosaic virus Potexvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 164 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313836 |
| タイトル（別表記） | Reaction of the Resistant Gene ml-o to Various Barley Powdery Mildew Strains |
|---|---|
| フルテキストURL | 003_001_011_016.pdf |
| 著者 | 部田 英雄| 武田 和義| |
| 抄録 | Eight powdery mildew strains, four collected from the field and the other four derived from a cross between two strains, were inoculated to seven mutant barley lines having the ml-o gene for powdery mildew resistance and their original varieties of barley. These eight powdery mildew strains showed a distinctive reaction to the nine testers having various resistant genes. Onthe other hand, the original barley varieties showed a similar reaction to all the powdery mildew strains inoculated, indicating that the genetic background of these varieties was similar. In comparison with the ml-o mutants and their original varieties, the type of lesion did not change in most cases, but the number of lesions decreased significantly without exception. In conclusion, the powdery mildew resistant gene ml-o does not affect the type of lesions but it reduces the number of lesions. |
| キーワード | Powdery mildew resistance Barley ml-o gene Pathogenicity |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 11 |
| 終了ページ | 16 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Studies on Host Selection, Development and Reproduction of Melanaphis sacchari (Zehntner) |
|---|---|
| フルテキストURL | 003_001_005_010.pdf |
| 著者 | 河田 和雄| |
| 抄録 | The host selection, development and reproduction of sugarcane aphid, Melanaphis sacchari (Zehntner) were studied. Both alate and apterous virginoparous adults showed a stronger tendency of preference to a susceptible strain of sorghum and Cuba grass, Sorghum helepense (Persoon) than a resistant strain of sorghum and Eulalia, Miscanthus sinensis (Anderss). The number of days for larval development was increased with a decrease in longevity and fecundity in aphids reared on resistant sorghum at 25℃ under a 16-hour photoperiodic condition. |
| キーワード | Sorghum Host selection Sugarcane aphids |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 5 |
| 終了ページ | 10 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Detection of the Viruses Occurring in Oriental Cymbidium in Japan |
|---|---|
| フルテキストURL | 004_002_149_162.pdf |
| 著者 | 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A survey of virus diseases occurring in Oriental Cymbidium collected from a commerical nursery and home garden in Japan was conducted in 1991-1994. Identification of the vurus was based on partcle morphology, symptomatology in indicator plants, ultrastructure of infected cells and serology. Four viruses, odontoglossum ringspot tabamovirus(ORSV), cymbidium mosaic potexvirus(CyMV), orchid fleck virus (ORV) and a previously underscribed spherical virus, were found in 27 out of 37 Cymbidium plants tested. ORSV was detected from 11 plants belinging to Cym. ensifolium, Cym. forrestii, Cym. goeringii, Cym. kanran, Cym. sinense and Cymbidium spp. showing chlorotic streaks and/or mild mosaic. CyMV was isolated from only one plant of Cymbidium sp. showing mosaic and necrotic spots on leaves. In negatibvely stained dip preparations from plants infected with ORSV and CyMV, rod shaped particles of ca. 310 nm and flexuous rod-shaped ca. 475 nm in length were observed, respectively. The viruses were reacted strongly with respective antiserum to each virus in immunosorbent electron microcopy and inderect ELISA. OFV was isolated from four plants of Cym. formosanum, Cym. kanran, Cym. sinense and Cymbidium sp. showing mosaic and necrotic flecks. The virus had non-enveloped, bullet-shaped particles about 40×120～150 nm in dip preparation. The undescribed spherical virus, ca. 28 nm diameter, was isolated from 11 plants of Cym. forrestii, Cym. goeringii and Cymbidium spp. showing stunting and chlorotic streaks on newly developed leaves. The virus was mechanically transmitted only to Cymbidium orchids. Previously, we designated it as cymbidium chlorotic mosaic sobemovirus(CyCMV)(Kondo et al,1994),as the virus was considered to be a new member of the genus Sobemovirsu. |
| キーワード | Oriental Cymbidium Odontoglossum ringspot tobamovirus Cymbidium mosaic potexvirus Orchid fleck virus Cymbidium chlorotic mosaic sobemovirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 149 |
| 終了ページ | 162 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313470 |
| タイトル（別表記） | カクヤリグサ科一年生雑草における数量分類学的研究 |
|---|---|
| フルテキストURL | 002_001_123_134.pdf |
| 著者 | Muhamad Ahmad Chozin| 佐藤 和広| 安田 昭三| |
| 抄録 | Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. |
| キーワード | Cyperus iria Cyperus microiria Cyperus amuricus Numerical taxonomy Speciation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 123 |
| 終了ページ | 134 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313639 |
| タイトル（別表記） | オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較 |
|---|---|
| フルテキストURL | 004_002_137_147.pdf |
| 著者 | 池上 正人| 井上 成信| |
| 抄録 | The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%. |
| キーワード | Tobamovirus Odontoglossum ringspot virus Nucleotide sequence Genome organization |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 137 |
| 終了ページ | 147 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |