異臭魚の発生と石油成分の食物連鎖 第2編 原油及び油処理剤懸濁海水で飼育した貝類へのパラフィン系炭化水素の移行

Short necked clams (Venerupis Amygdala japonica) were maintained in a controlled laboratry environment in sea water containing suspension of crude oil and then transferred to clean sea water. Transfering and disappearance of n-paraffins in the soft body of short necked clams were examined by gaschromatography. n-Hexane solution after clean up treatment was treated by distribution method with n-hexane and acetonitrile and were separated n-paraffins and aromatic hydrocarbons. Short necked clams were reared in oil disperser or supplement with C(20), C(22), C(24) of n-paraffins and n-paraffins in soft body of short necked clams were analyzed by gaschromatography. The following reasults were obtained. 1) C(13)-C(2O) of n-paraffins in crude oil were transfered in the soft body of short necked clams. The concentration of n-paraffins increasd with rearing time in crude oil suspension and decreased with crean sea water. The rate of disappearance of C(10)-C(15) in n-paraffin were decreased faster than C(16)-C(20) of n-paraffins. 2) Short necked clams were reared in the crude oil suspension (1,000 ppm) for 3 days. n-Hexane solution after clean up was treated by distribution method. n-Paraffins remains in n-hexane and di-, tri-cyclic aromatic hydrocarbons transfered from n-hexane solution to acetonitrile solution. 3) Short necked clams were reared in oil disperser solution (100 ppm). Gaschromatograms of soft body of short necked clams showed the presence of C(12)-C(16) of n-paraffins. Short necked clams were reared in oil diperser solution supplemented with C(20), C(22), C(24) of n-paraffins. Gaschromatograrm of soft body of short necked clams showed the presence of C(20), C(22), C(24) of n-paraffins except C(12)-C(16) of n-paraffins of oil disperser.