塚原宏一

Validity of Mothers’ Reports of Children’s Weight in Japan

JaLCDOI	10.18926/AMO/54500
フルテキストURL	70_4_255.pdf
著者	Nosaka, Nobuyuki\| Fujiwara, Takeo\| Knaup, Emily\| Okada, Ayumi\| Tsukahara, Hirokazu\|
抄録	Estimation methods for pediatric weight have not been evaluated for Japanese children. This study aimed to assess the accuracy of mothersʼ reports of their childrenʼs weight in Japan. We also evaluated potential alternatives to the estimation of weight, including the Broselow tape (BT), Advanced Pediatric Life Support (APLS), and Parkʼs formulae. We prospectively collected cross-sectional data on a convenience sample of 237 children aged less than 10 years who presented to a general pediatric outpatient clinic with their mothers. Each weight estimation method was evaluated using Bland-Altman plots and by calculating the proportion within 10 and 20 of the measured weight. Mothersʼ reports of weight were the most accurate method, with 94.9 within 10 of the measured weight, the lowest mean difference (0.27kg), and the shortest 95 limit of agreement (－1.4 to 1.9kg). The BT was the most reliable alternative, followed by APLS and Parkʼs formulae. Mothersʼ reports of their children ʼs weight are more accurate than other weight estimation methods. When no report of a childʼs weight by the mother is available, BT is the best alternative. When an aged-based formula is the only option, the APLS formula is preferred.
キーワード	body weight child estimation techniques mothers, parents
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2016-08
巻	70巻
号	4号
出版者	Okayama University Medical School
開始ページ	255
終了ページ	259
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2016 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	27549669
Web of Science KeyUT	000384748600004

血管内皮機能を対象にした基礎および臨床医学研究

タイトル（別表記）	Basic and clinical research regarding vascular endothelial function
フルテキストURL	127_187.pdf
著者	塚原宏一\|
キーワード	アルギニン代謝一酸化窒素ガス生物学血管内皮学酸化ストレス
出版物タイトル	岡山医学会雑誌
発行日	2015-12-01
巻	127巻
号	3号
開始ページ	187
終了ページ	195
ISSN	0030-1558
言語	日本語
著作権者	Copyright (c) 2015 岡山医学会
論文のバージョン	publisher
DOI	10.4044/joma.127.187
NAID	130005116819

Inhibitory Effects of Edaravone, a Free Radical Scavenger, on Cytokine-induced Hyperpermeability of Human Pulmonary Microvascular Endothelial Cells:A Comparison with Dexamethasone and Nitric Oxide Synthase Inhibitor

JaLCDOI	10.18926/AMO/53674
フルテキストURL	69_5_279.pdf
著者	Saito, Yukie\| Fujii, Yousuke\| Yashiro, Masato\| Tsuge, Mitsuru\| Nosaka, Nobuyuki\| Yamashita, Nobuko\| Yamada, Mutsuko\| Tsukahara, Hirokazu\| Morishima, Tsuneo\|
抄録	Lung hyperpermeability affects the development of acute respiratory distress syndrome (ARDS), but therapeutic strategies for the control of microvascular permeability have not been established. We examined the effects of edaravone, dexamethasone, and N-monomethyl-L-arginine (L-NMMA) on permeability changes in human pulmonary microvascular endothelial cells (PMVEC) under a hypercytokinemic state. Human PMVEC were seeded in a Boyden chamber. After monolayer confluence was achieved, the culture media were replaced respectively by culture media containing edaravone, dexamethasone, and L-NMMA. After 24-h incubation, the monolayer was stimulated with tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β). Fluorescein-labeled dextran was added. Then the trans-human PMVEC leak was measured. Expressions of vascular endothelial-cadherin (VE-cadherin) and zonula occludens-1 protein (ZO-1) were evaluated using real-time quantitative polymerase chain reaction and immunofluorescence microscopy. The results showed that TNF-α＋IL-1β markedly increased pulmonary microvascular permeability. Pretreatment with edaravone, dexamethasone, or L-NMMA attenuated the hyperpermeability and inhibited the cytokine-induced reduction of VE-cadherin expression on immunofluorescence staining. Edaravone and dexamethasone increased the expression of ZO-1 at both the mRNA and protein levels. Edaravone and dexamethasone inhibited the permeability changes of human PMVEC, at least partly through an enhancement of VE-cadherin. Collectively, these results suggest a potential therapeutic approach for intervention in patients with ARDS.
キーワード	pulmonary microvascular endothelial cells permeability edaravone vascular endothelial-cadherin zonula occludens-1 protein
Amo Type	Original Article
出版物タイトル	Acta Medica Okayama
発行日	2015-10
巻	69巻
号	5号
出版者	Okayama University Medical School
開始ページ	279
終了ページ	290
ISSN	0386-300X
NCID	AA00508441
資料タイプ	学術雑誌論文
言語	英語
著作権者	CopyrightⒸ 2015 by Okayama University Medical School
論文のバージョン	publisher
査読	有り
PubMed ID	26490025
Web of Science KeyUT	000365519600004

マウスインフルエンザ肺炎におけるレドックス制御蛋白チオレドキシン（TRX-1）の治療的効果

著者	八代将登\| 塚原宏一\| 松川昭博\| 山田睦子\| 藤井洋輔\| 長岡義晴\| 津下充\| 山下信子\| 伊藤利洋\| 山田雅夫\| 増谷弘\| 淀井淳司\| 森島恒雄\|
発行日	2013-08-01
出版物タイトル	岡山医学会雑誌
巻	125巻
号	2号
資料タイプ	学術雑誌論文

塚原 宏一