The influences of cortical hormones on the secretory ability of fructose and citric acid in semen were investigated with castrated mouse. The experiments were carried out exactly the same as stated in repost 1 with castrated mouse. 1. The weight of seminal vesicles and the contents of fructose and citric acid showed obviously decreases by the castration, but these were returned to normal and paralleled to the amounts of testosterone to be administrated in castrated mouse. 2. The weight of seminal vesicles and the contents of fructose and citric acid were also increased by the administration of ACTH and cortisone in castrated mouse, but the effects of these hormones were extremely slight comparing with testosterone.