result 40 件
| JaLCDOI | 10.18926/AMO/30846 |
|---|---|
| FullText URL | fulltext.pdf |
| Author | Suwa, Kiichi| |
| Abstract | The relationship between alveolar macrophages and spheroid alveolar epithelial cells was studied with the electron microscope after injection of squid-ink solution into the trachea of the mouse. At 20 hours after injection of squid-ink solution slight degeneration was evident in alveolar macrophages with sepia-melanin particles being phagocytized with partial digestion by lysosmes. Furthermore, hardly any changes were seen in mitochondria and inclusion bodies of the spheroid alveolar epithelial cells. In contrast, at one week after injection of squid-ink solution, almost all alveolar macrophages were degenerated with destruction of the ectoplasm in which the ingested sepia-melanin particles were digested by lysosomes into fine particles, and the mitochondria of spheroid alveolar epithelial cells were degenerated and the inclusion bodies were hardly formed. At three weeks after injection of squid-ink solution, alveolar macrophages as well as speroid alveolar epithelial cells showed almost complete recovery of functional structure. As the phagocyte in the alveolar space, neutrophile leucocytes were also observed in addition to the so-called alveolar macrophage. |
| Amo Type | Article |
| Publication Title | Acta Medica Okayama |
| Published Date | 1977-02 |
| Volume | volume31 |
| Issue | issue1 |
| Publisher | Okayama University Medical School |
| Start Page | 15 |
| End Page | 34 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| Content Type | Journal Article |
| language | English |
| File Version | publisher |
| Refereed | True |
| PubMed ID | 140589 |
| NAID | 120002305572 |
| Author | Aisaka, Tadakazu| |
|---|---|
| Published Date | 1962-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume74 |
| Issue | issue8-9 |
| Content Type | Journal Article |
| Author | Tawara, Jutaro| |
|---|---|
| Published Date | 1968-08-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume80 |
| Issue | issue7-8 |
| Content Type | Journal Article |
| Author | Miwa, Yasuhiko| |
|---|---|
| Published Date | 1967-12-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume79 |
| Issue | issue11-12 |
| Content Type | Journal Article |
| Author | Suwa, Kiichi| |
|---|---|
| Published Date | 1976-08-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume88 |
| Issue | issue7-8 |
| Content Type | Journal Article |
| Author | Taguchi, Hirokuni| Sanada, Hiroshi| Tanaka, Toshio| Hayashi, Takehiko| Miyoshi, Isao| Kitayama, Minoru| |
|---|---|
| Published Date | 1975-10-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume87 |
| Issue | issue9-10 |
| Content Type | Journal Article |
| Author | Tomoyasu, Satoshi| |
|---|---|
| Published Date | 1975-04-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume87 |
| Issue | issue3-4 |
| Content Type | Journal Article |
| Author | Akatsuka, Kazuya| |
|---|---|
| Published Date | 1978-04-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume90 |
| Issue | issue3-4 |
| Content Type | Journal Article |
| JaLCDOI | 10.18926/15254 |
|---|---|
| Title Alternative | ヒスチジンタグを持つホスファカンコア蛋白の大腸菌での発現と精製 |
| FullText URL | 009_2_105_111.pdf |
| Author | Ito, Sekiko| Okamoto, Motoi| Mori, Shuji| |
| Abstract | Specific regions of core protein of phosphacan, one of the chondroitin sulfate proteoglycans, were expressed as fusion proteins with histidine-tag (His-tag) in Escherichia coli (E.coli) and were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) matrix. cDNA fragments encoding amino acid residues 343-446 (P3) and 1-340 (P4) of phosphacan core protein were amplified by polymerase chain reaction from E18 rat brain mRNA as template. The amplified products were subcloned into pQE30 vector and were introduced into E.coli strain M15 [pREP4] for the expression. The His-tagged fusion proteins were expressed by cultivating the transformants at 37℃ for 5h in the presence of 1mM IPTG. His-tagged P3 fusion protein (His-P3) was expressed as soluble form, and was purified using Ni-NTA matrix. His-tagged P4 fusion protein (His-P4) which was sequestered into insoluble inclusion bodies was treated with 8.0M urea to solubilize, and then was purified under denaturing conditions. |
| Keywords | phosphacan (ホスファカン) core protein (コア蛋白) His-tagged proteins recombinant protein (融合蛋白) |
| Publication Title | 岡山大学医療技術短期大学部紀要 |
| Published Date | 1999-02-26 |
| Volume | volume9 |
| Issue | issue2 |
| Start Page | 105 |
| End Page | 111 |
| ISSN | 0917-4494 |
| language | English |
| File Version | publisher |
| NAID | 120002307302 |
| Author | Kuyama, Keisuke| |
|---|---|
| Published Date | 1991 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume103 |
| Issue | issue11-12 |
| Content Type | Journal Article |
| Author | Shibata, Jun| |
|---|---|
| Published Date | 1966-06-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume78 |
| Issue | issue6 |
| Content Type | Journal Article |
| Author | Shibata, Jun| |
|---|---|
| Published Date | 1966-06-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume78 |
| Issue | issue6 |
| Content Type | Journal Article |
| Author | Ikeda, Kazuhiko| |
|---|---|
| Published Date | 1966-06-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume78 |
| Issue | issue6 |
| Content Type | Journal Article |
| Author | Yamamoto, Masakazu| |
|---|---|
| Published Date | 1966-05-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume78 |
| Issue | issue4-5 |
| Content Type | Journal Article |
| Author | Oda, Teruyuki| |
|---|---|
| Published Date | 1991 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume103 |
| Issue | issue11-12 |
| Content Type | Journal Article |
| Author | 横田 修| 寺田 整司| 石津 秀樹| 黒田 重利| |
|---|---|
| Published Date | 2004-09-30 |
| Publication Title | 岡山医学会雑誌 |
| Volume | volume116 |
| Issue | issue2 |
| Content Type | Journal Article |
| Title Alternative | Ixia から分離された bean yellow mosaic virus |
|---|---|
| FullText URL | 004_002_201_213.pdf |
| Author | Tsuji, Toshiya| Maeda, Takanori| Kondo, Hideki| Inouye, Narinobu| |
| Abstract | A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. |
| Keywords | Ixia hybrida Bean yellow mosaic virus Potyvirus |
| Publication Title | 岡山大学資源生物科学研究所報告 |
| Published Date | 1996 |
| Volume | volume4 |
| Issue | issue2 |
| Start Page | 201 |
| End Page | 213 |
| ISSN | 0916-930X |
| language | English |
| File Version | publisher |
| NAID | 120002313395 |
| Author | Francico Javier Diaz Corrales| |
|---|---|
| Published Date | 2006-03-24 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| Author | Takehisa, Yasushi| |
|---|---|
| Published Date | 2001-03-31 |
| Publication Title | |
| Content Type | Thesis or Dissertation |
| FullText URL | O003631.pdf |
|---|---|
| Author | 寺田 整司| |
| Published Date | 2001-09-30 |
| Content Type | Thesis or Dissertation |
| Grant Number | 乙第3631号 |
| Granted Date | 2001-09-30 |
| Thesis Type | Doctor of Philosophy in Medical Science |
| Grantor | 岡山大学 |
| language | Japanese |
