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In the present experiment, it has been noted that clonizing epithelial-like cells of the intestine 407 were more susceptible to SV-40 virus than normal fibroblasts in primary human cell cultures. In the early stage of the infection the cell growth was enhanced by the inoculation of DNA virus but many cells died, showing lysis characterized by CPE, clumping of chromatin and formation of inclusion bodies. On the other hand, the cells surviving infection have given rise to virus-free long term cultures and cellular responses to the virus characterized by cell proliferation which is. classified in four phases. (Phase. I: infection and cell alteration. Phase. II: crisis. Phase. III: fibro-reticulum cell formation. Phase. IV: recovery and proliferation). The most remarkable morphological characteristic was fibroblastic cell alteration from epithelial cells at 5 weeks of virus inoculation. By this study an interesting generalization of human epithelial-like cells can be made about the differentiation of the transformed cells in relation to SV-40 virus and it has been shown that an established human cell line is still susceptible to the reverting action of the SV-40 virus.

There are many electron microscopic observations of the cells infected with measles virus (1-6), and all of them appear to be concerned mainly with observation on the inclusion bodies and not any seems to have described the morphology of mature virus particles located within the infected cell.

Electron microscopy of the body wall of Opisthorchis viverrini shows the integument which is connected to the epidermal cell with fine protoplasmic tubules, to form a syncytium, as in Clonorchis sinensis and other trematodes. Vacuole-like secretory granules are distributed in the matrix of the integument, and mitochondria are arranged at the proximal outer surface of the integument. The crystalline inclusions are observed in the perinucleus of some epidermal cells.

We report the first case in Japan, i.e., the first case among oriental subject of Farber's disease. This is a rare disorder of lipid metabolism in infancy subsequent to a genetically-determined defect in ceramide degradation. Main features are characterized clinically by hoarseness, joint swelling, subcutaneous nodules and retarded psychomotor development. Lipid analysis and pathological investigation on the material obtained from a subcutaneous nodule confirmed clearly the presence of ceramide and intracytoplasmic inclusion bodies characteristic for Farber's disease. In this case, we experienced also corneal opacity and striking abnormalities in electroencephalogram, which have apparently not been noticed in the 17 cases hitherto reported.

The effect of an intravenous injection of squid-ink (sepia-melanin) solution on adult mouse spheroid alveolar epithelial cells was observed by the electron microscope. Sepia-melanin particles were seen in all alveolar wall cells examined that seems to suggest the entrance of sepia-melanin particles into the spheroid alveolar epithlial cells from the alveolar blood capillary. In cases of large penetrations of sepia-melanin particles into spheroid alveolar epithelial cells, a greater increase was found in the intramitochondrial granules. In addition, the so-called inclusion body believed to be formed by the degeneration of mitochondria had very high electron density and its quantity was abundant. On the contrary, in cases where the quantity of sepia-melanin entrance into the spheroid alveolar epithelial cell was small, neither an increase of intramitochondrial granules, an increase of the electron density nor an increase in the quantity of specific inclusion body was found.

Electron microscope observations were conducted on the relationship between mitochondria and inclusion body in mice spheroid alveolar epithelial cells after injection of trypan blue, an acidic dye and Alcian blue 8GS, a basic dye, by vital staining procedures. When both dyes were injected, the mitochondria of the spheroid alveolar epithelial cell became degenerated; however, in injection of only trypan blue, the cristae showed an increase in electron density. In injection on only Alcian blue 8GS, the cristae showed negative contrast. In most cases the trypan blue particles did not enter into mitochondria, whereas particles of Alcian blue 8GS sometimes entered into the mitochondria. When trypan blue particles entered mitochondria, deposits were not evident in the inclusion body, whereas when Alcian blue particles entered mitochondria deposits were seen in the inclusion body. In both of these cases only a few inclusion bodies were formed so that only traces or no inclusion bodies with vacuolar appearance were observed. From these findings it is suggested that mitochondria maybe convert to inclusion bodies.