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Title Alternative 幼苗期と成熟期のオオムギ系統間における禾穀類アブラムシの密度とグラミン含量の関係
FullText URL 004_001_049_058.pdf
Author Moharramipour, Saeid| Murata, Shin-ichi| Kanehisa, Katsuo| Tsumuki, Hisaaki|
Abstract The relationship between the indole alkaloid gramine concentration and aphid population was examined seedling and maturation stages in 14 barley lines of Hordeum spontaneum and H. unlgare. The density of Schizaphis graminum did not differ significantly with the gramine concentration in the seedling in the greenhouse. However, the population of Rhopalosiphum padi sometimes differed with the seedling. The plant resistance to the natural infestation of cereal aphids was obvious at the heading stage. There was a negative correlation between the high population density of aphids and gramine concentration. The gramine concentration was high in matured resistant resistant lines, especially wild lines, as compared with susceptible lines due to higher biodegradation activity.
Keywords Barley Resistance Gramine Cereal aphids
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue1
Start Page 49
End Page 58
ISSN 0916-930X
language English
File Version publisher
Title Alternative QTL Analysis for Expressivity of Hull-cracked Grain in Two-rowde Spring Barley
FullText URL 005_002_183_191.pdf
Author Kanatani, Ryouichi| Takahashi, Hidekazu| Takeda, Kazuyoshi|
Abstract The exposure of the caryopsis through lemma and palea is called 'hull-cracked grain', which lowers the germinability and grade of malting barley. To breed new varieties with a low occurence of hull-cracked grains,quantitative trait loci(QTL) analysis was conducted using 146 doubled haploid lincs derived from Harrington × TR306.Interval mapping analysis revealed three significant QTLs on chromosomes 3H,5H and 7H. About 37% of the variation of the expressvity of hull-cracked grains was explained by these three QTLs in a spikelet-thinning condition where 1/4 of the spikelets were thinned at the flowering time. On the contraty the QTL on 5H was silent in normal or non-treated condition and 23% of the variation was determined by the QTLs on 3H and 7H. Some of the QTLs detected here had a pleiotropic effect on the grain size.
Keywords Barley Quality Hull-cracked grain QTL analysis
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 183
End Page 191
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Relationship between Resistance to Cereal Aphids and Gramine Concentration in Beer Barley
FullText URL 004_001_043_048.pdf
Author Tsumuki, Hisaaki| Kasahara, Toshihiko| Kanehisa, Katsuo| Saeid, Moharramipour|
Abstract To beer barley resistance to cereal aphids, the relationship between the aphid density and gramine concentration was examined in various barley lines including beer barley cultivars. All beer barley cultivars tested were resistant or moderately resistant to the aphids, especially Asahi 5 and Haruna Nijo were similar to a wild line, H603 (W603), for checking resistant. However, these beer barleys contained much less gramine than the wild barley line, suggesting that another resistant factor(s) affects cereal aphid density.
Keywords Beer barley Cereal aphid Resistance Gramine
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue1
Start Page 43
End Page 48
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Vertical Structure of Temperatures of Tree's Leaves and Gate Wall at Rashomon Doline
FullText URL 005_002_169_181.pdf
Author Miyashita, Koichi| Maitani, Toshihiko|
Abstract Temperatures of tree's leaves and gate wall were measured in the summer of 1996 at doline. Diurnal fluctuations of leaf temperatures were greater, the higher the position of leaf in the doline, and they showed maximum temperatures later, the deeper the leaf position. The leaf temperature of trees growing in the first collapse doline was uniform in the horizontal direction and had a stable thermal layer. The difference between leaf temperatures at a depth of 20 m and 11 m was about 12℃ around noon. The leaf temperature was always higher at the upper part than at the lower part of the doline throughout the day. Daily fluctuation of wall temperature was small,compared with that of leaf temperature. The vertical profile of wall temperature was similar to that of leaf temperature. The warm water flowing down from the upper part of the doline influenced the soil temperature in the lower part of the doline. The thermal imagery elucidated the peculiar vertical structure of leaf and wall temperature formed in the summer at Rashomon doline.
Keywords Rashomon Doline Leaf temperature Wall temperature Thermal image
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 169
End Page 181
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative トウモロコシに含まれるDIMBOAとアブラムシ抵抗性の関係
FullText URL 004_001_033_042.pdf
Author Rustamaki, Maqsood A| Kanehisa, Katsuo| Tsumuki, Hisaaki| Shiraga, Takashi|
Abstract 2,4-Dihydrox-7-methoxy1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid is considered to be one of the components responsible for the resistance to pest insects in cereal plants. The relationship between concentration of DIMBOA and aphid infestation on 21 corn lines was investigated in 1990 and 1991. DIMBOA was detected in leaves od all corn lines tested, contained larger amounts in young plants and gradually decreased with growth. This property was the same as observed in wheat. A more than ten times difference in DIMBOA concentration was observed in corn lines. However, there was no positive correlation between DIMBOA concentration and aphid density. Most of the lines used had resistance to aphids. Resistance may be built together with other components such as (E)-aconitic acid.
Keywords Apfid Resistant substance Hydroxamic acid DIMBOA Corn
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue1
Start Page 33
End Page 42
ISSN 0916-930X
language English
File Version publisher
Title Alternative メダカハネカクシの尾部分分泌物
FullText URL 004_001_025_031.pdf
Author Kanehisa, Katsuo| Tsumuki, Hisaaki|
Abstract Rove beetles have a pair of pygidial excretory organs, each group secretes characteristic substances in a peculiar manner. The genus Stenus has a pair of pygidial organs, eversible by immersion in diethyl-ether at both sides of the anus. S. anthoracinus, S. melanarius vercecundus and S. rufescens were collected at ponds and river shores, and S. alienus was collected at tobacco and vegetable fields. Excretory substances were secreted in the diethylether, and identified by gas chromatography and mass spectrometry. Stenusin [N-Ethyl-3-(2-methylbutyl) piperidine], 1,8-cineole, isopiperitenol and 6-Me-5-hepten-2-one were identified. These may probably act as defensive substances against small attacking animals and microorganisms, and act as water suface moving active agents.
Keywords Rove beetles Stenus Excretory secretion Pygidial organ Defensive secretion
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue1
Start Page 25
End Page 31
ISSN 0916-930X
language English
File Version publisher
Title Alternative Habenaria Mosaic Virus, A New Member of Potyvirus from Habenaria radiata Orchid
FullText URL 005_002_155_168.pdf
Author Inouye, Narinobu| Maeda, Takanori| Mitsuhata, Koji| Gara, I Wayan|
Abstract A potyvirus, isolated from Habenaria radiaata showing severe mosaic in the leaves named habenaria mosaic virus(HaMV). HaMV was transmitted by inoculation of sap to 6 of 41 species from 4 of 12 families. Systemic infection was cauded in Habenaria radiata, and local infection was produced in Chenopodium amaranticolor, C. quinoa, Spinacia olracea, Tetragonia expansa and Gomphrena globosa. HaMV was also transmitted by Myzus persicae in a non persistent manner, but not by Aphis gossypii. The virus has filamentous particles of c. 750×13nm. Sap from inoculated C. quinoa was infective after dilution to 10-5 but not 10-6, after 10 min at 60℃ but 65℃, or after 8-16 days at 20℃.In ultrathin sections of diseased leaves of Habenaria radiata and inoculated C. quinoa, virus particles were found to disperse in the cytoplasm. Cylindrical inclusions were present in the cytoplasm of infected cells, as pinwheels, scrolls or tubes. The inclusions observed in negatively stained crude sap preparations exhibited fine linear striation at about 5.2 nm intervals.The antiserum to HaMV produced by immunizing a rabbit had a titer of 1/512(precipitin test). HaMV showed distant serological relationships to potyvirus;alstroemeria mosaic virus, bean yellow mosaic virus, calanthe mild mosaic virus, clover yellow vein virus, dendrobium mosaic virus, freesia mosaic virus, turnip mosaic virus and watermelon mosaic virus 2.
Keywords Habenaria orchid Habenaria mosaic potyvirus Habenaria mosaic disease
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 155
End Page 168
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現
FullText URL 005_002_145_153.pdf
Author Sugimoto, Manabu| Kawai, Fusako|
Abstract A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one.
Keywords Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 145
End Page 153
ISSN 0916-930X
language English
File Version publisher
Title Alternative イネ幼植物から調整した細胞壁に含まれるペクチン質の性状
FullText URL 005_002_135_144.pdf
Author Konno, Haruyoshi| Tsumuki, Hisaaki| Yamasaki, Yoshiki|
Abstract Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4~0.6% of the starch-free cell walls.
Keywords Cell wall Oryza sativa Pectic polymer
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 135
End Page 144
ISSN 0916-930X
language English
File Version publisher
Title Alternative Secretion of Defensive Substance by Carabidae and Brachinidae
FullText URL 004_001_009_023.pdf
Author Kanehisa, Katsuo|
Abstract The secretion of defensive substances by more than 250 species of carabid and brachinid beetles was investigated, and discussed from a phylogenetical viewpoint. The morphological of secretion glands and secreted components were identified in the genus level. Specific subgenus and species had peculiar characteristics. The pair of defensive organs consisted of many small synthetic lobes, large reservoirs, collecting ducts from lobes to reservoirs and secretion ducts from reservoirs. The pair dust opened at the last sternal intersegmental membrane. There was species specificity in the reservoir shape, synthetic lobe shape and entering site of the collecting ducts into reservoirs. The spherical shape lobes produced short chain fatty acids, the elongated shape lobes produced m-cresol and the thick board shape lobes produced benzoquinones. Two types of fatty acid-secreting beetles were found, one secreted formic acid, and the other mixed short chain fatty acids. These short chain fatty acids were metabolized from some amino acids, methacrylic acid was from valine; tiglic acid, α-ethyacrylic acid and angelic acid were from isoleucine; senecioic acid was from leucine and crotonic acid was from lysine. These unasaturated acids were sometimes detected with a trace amount of corresponding saturated acids. A few species secreted larger amounts of saturated acids with a trace amounts of unsaturated acids. Fatty acid composition was generally genus-specific. In a few cases, however, species-specific acid composition was also observed. m-Cresol-secreting beetles were observed only in Panagaeni and Chlaeniini except subgenus Chlaenius Chlaeniini except subgenus Chlaenius Chlaeniellus beetle. 1,4-Benzoquinone and 2-methyl-1,4-benzoquinone-secreting beetles were observed in subgenus C. Chlaeniellus. This beetle secreted these substances at body temperature from a reservoir with no sound. Brachinidae beetles, called bombardier beetles, have a pair of brownish reaction chamber connected with a reservoir. They sprayed the two benzoquinones at about 100℃ making sound.
Keywords Defensive secreting substance Synthetic organ Carabidae Brachinidae Phylogenetic relation
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue1
Start Page 9
End Page 23
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Purification and Properties of α-Glucodidase from Taro Tuber
FullText URL 005_002_129_134.pdf
Author Mashima, Hideyuki| Yamasaki, Yoshiki| Konno, Haruyoshi|
Abstract α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose.
Keywords α-glucosidase taro tuber Colocasia esculanta Shott
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 129
End Page 134
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative ナメクジのα-グルコシダーゼの精製と性質
FullText URL 005_002_121_127.pdf
Author Yamasaki, Yoshiki| Konno, Haruyoshi|
Abstract Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly.
Keywords Slug Incilaria bilineata α-glucosidase
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1998
Volume volume5
Issue issue2
Start Page 121
End Page 127
ISSN 0916-930X
language English
File Version publisher
Title Alternative Varietal Variation and Effects of Some Major Genes on Salt Tolerance in Barley Seedlins
FullText URL 003_001_071_081.pdf
Author Mano, Yoshiro| Takeda, Kazuyoshi|
Abstract In order to select the salt tolerant cultivars, and to evaluate the effects of some major genes on salt tolerance in barley, 5,182 varieties and 368 isogenic lines were tested by exposing to 1.0 and 0.5M sodium chloride at the seedling stage. The average rating of salt tolerance was higher in varieties from Korea and Japan , but lower in varieties from Nepal and Southwest Asia. When the varieties were grouped by several morphological characters, six-rowed, covered, E type of nonbrittle rachis and uzu type groups were more tolerant than two-rowed, naked, W type and non-uzu type counterparts, respectively. A comparison between the isogenic pairs revealed that the uzu type was more tolerant than non-uzu type, but other major gene pairs did not show any obvious differences. These findings indicate that the uz gene affects the salt tolerance, and diferences between varietal groups characterized by other major genes are due to the difference in the genetic background among these varieties.
Keywords Barley Seedling Salt tolerance Screening Isogenic line
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue1
Start Page 71
End Page 81
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Diallel Analysis of 1,000-grain Weight in Two-rowed Barley Varieties
FullText URL 003_001_063_070.pdf
Author Wu, Jiri| Takeda, Kazuyoshi|
Abstract The inheritance of 1,000-grain weight of two-rowed barley varieties was examined using a reciprocal F1 cross and a half F2 diallel cross among eight parents. The F1 generation was examined in five different ripening conditions i.e., (Ⅰ) control, (Ⅱ) leaf cut, in which all leaves were cut at the heading time, (Ⅲ) Spikelet thinned, in which a quarter of the spikelets were thinned at the heading time, (Ⅳ) dry, in which the plants were grown in a vinyl-film house without irrigation, and (Ⅴ) wet, in which the plants were mist-irrigated every two house in the daytime after the heading to the harvest. The experiment was repeated twice. The F2 populations were grown in the ordinary condition. The average 1,000-grain weight was largest in the dry plot followed by spikelet thinned, control, leaf cut, and wet plots. The 1,000-grain weight was predominantly controlled by the additive genes. The dominance effect was also significant. The large grain weight was controlled by the overdominant genes. Epistasis among the genes controlling the 1,000-grain weight was significant, but the maternal effect was absent. In the F1 diallel, heritability was 0.3~0.6 and 0.2~0.5 in a broad and a narrow sense, respectively. In the F2 diallel, it was 0.9 and 0.6 in a broad and a narrow sense, respectively. In each of the 28 F2 populations, the broad sense heritability was estimated as 0.3~0.8.
Keywords Barley Grain size Diallel analysis
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue1
Start Page 63
End Page 70
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Purification and Characterization of α-Glucosidases from Spinach Seeds
FullText URL 004_002_239_252.pdf
Author Sugimoto, Manabu| Furui, Satoshi| Suzuki, Yukio|
Abstract Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Ⅰand Ⅱ showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases Ⅲ and Ⅳ showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase Ⅲ serum precipitated specifically with α-glucosidase Ⅲ.
Keywords α-Glucosidase Spinach Seed Spinacia oleracea L. Molecular form
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue2
Start Page 239
End Page 252
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Production of Strain of Erysiphe graminis f. sp. tritici That Are Pathogenic on Barley and Agropyron
FullText URL 004_002_229_237.pdf
Author Heta, Hideo| Inouye, Narinobu|
Abstract Two isolates of Erysiphe graminis f. sp. tritici, T3 and T4, were crossed and selected for pathogenicity. The corssing and selection process were repeated five times with the progenies. Isolates of each generation were inoculated on the non-hosts, Hordeum vulgare and Agropyron tsukushiense var. transiens OHWI, and parasitism and pathogenicity were investigated with respect to host specificity.As shown in Tables 2 to 5, the process of crossing and selection produced isolates which were pathogenic on barley cultivars, 'Kagoshimahadaka' or 'Russian 9' or Agropyron tsukushiense ecotypes 'Agropyron Early ecotype' or 'Agropyron Yezoense 3'.Factors affecting the degree of pathogenicity appear to be under polygenic control. The cumulative effect of minor genes may play a role in the differentiation of formae speciales suggesting a new concept of pathogenicity in the powdery mildew of grasses.
Keywords Wheat powdery mildew Host specific Pathogenicity Properties Barley Agropyron
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue2
Start Page 229
End Page 237
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Comparison between shoot Regeneration Ability in Calli Derived from Immature Embryos of Wild and Cultivated Barley
FullText URL 003_001_055_062.pdf
Author Rikiishi, Kazuhide| Takeda, Kazuyoshi| Yasuda, Shozo|
Abstract The variation in shoot regeneration obility of calli derived from immature embryos was examined in 95 wild strains, 82 of which were of Hordeum spontaneum and 13 of which were H. agriocrithon, and 87 cultivated varieties collected from various countries or regions of the world. In 85 strains of the wild species, a number of calli regenerated shoots, and their proportion ranged from 1.2% to 75.7%. The average percentage of shoot regenerating calli was 21.7% among the strains that formed calli, 11.5% of which regenerated green and 10.2% albino shoots. On average, 21.4% and 23.9% calli regenerated shoots in H. spontaneum and H. agriocrithon, respectively and there was no significant difference between these values. A significant difference in the percentage of shoot regenerating calli was found among six variants (dawense, ishnatherum, laguncliforme, paradoxon, proskowetzii, spontaneum) which were comprised in H. spontaneum. In 73 varieties of cultivated species, there were shoots regenerating calli likely to wild species, and their proportion ranged from 3.2% to 85.5%. The average percentage of shoot regenerating calli was 25.4%, 22.0% of which regenerated green and 3.4% of which regenerated albino shoots. There was a significant difference in percentage of green shoots regenerating calli against shoots regenerating ones between the wild (53.0%) and cultivated species 886.6%). The two kinds of non-brittle rachis genotypes, Bt bt2 and bt Bt2 are one of the key characters distinguishing the oriental and occidental types of cultivated barley. The average percentages of shoot regenerating calli were 16.2% and 32.3% for the genotypes Bt bt2 and bt Bt2, respectively, suggesting that there is a geographical variation in the shoot regeneration ability of calli in the cultivated species. By contrast, the oriental and occidental strains of wild species showed no difference in the shoot regeneration ability of calli. The geographical variation of shoot regeneration ability differed significantly between wild and cultivated species. This suggests that the geographical variation of shoot regeneration ability occurred after the cultivation of the barley was established.
Keywords Hordeum spontaneum Hordeum agriocrithon Tissue culture Geographical variation Shoot regeneration ability
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue1
Start Page 55
End Page 62
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative Some Properties of Azuki Bean Mosaic Virus Isolated from Soybean Plants in Ibaraki Prefecture
FullText URL 004_002_215_227.pdf
Author Takahashi, Kokichi| Inouye, Narinobu| Maeda, Takanori| Mitsuhata, Koji| Chida, Shigeki| Takahashi, Yoshiyuki|
Abstract Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40~50% less than that of healthy plants.
Keywords Potyvirus Azuki bean mosaic virus Glycine max Properties
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1996
Volume volume4
Issue issue2
Start Page 215
End Page 227
ISSN 0916-930X
language Japanese
File Version publisher
Title Alternative 大麦網斑病抵抗性の選抜効果
FullText URL 003_001_043_053.pdf
Author Sato, Kazuhiro| Takeda, Kazuyoshi|
Abstract Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy.
Keywords Net blotch Pyrenophora teres Selection Barley Disease resistance
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue1
Start Page 43
End Page 53
ISSN 0916-930X
language English
File Version publisher
NAID 120004537480
Title Alternative ダイズ種子の冠水抵抗性の品種間差とダイアレル分析
FullText URL 003_001_035_041.pdf
Author Hou, Fwu-Fenn| Thseng, Fu-Sheng| Wu, Shu-Tu| Takeda, Kazuyoshi|
Abstract Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations.
Keywords Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference
Publication Title 岡山大学資源生物科学研究所報告
Published Date 1995
Volume volume3
Issue issue1
Start Page 35
End Page 41
ISSN 0916-930X
language English
File Version publisher