result 19466 件
| Title Alternative | Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis |
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| FullText URL | poalas_033_031_034.pdf |
| Author | Aizawa, Sayaka | Higaki, Yuriko| Ogoshi, Maho| Takeuchi, Sakae| Takahashi, Sumio| |
| Abstract | Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development. |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 31 |
| End Page | 34 |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Taxonomic status of Aco strain derived from spiny mice |
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| FullText URL | poalas_033_022_025.pdf |
| Author | Mochida, Hitomi| Mekada, Kazuyuki| |
| Abstract | Spiny mice are useful in regenerative research and as a model for precocial animals. In the present study, we investigated the taxonomic status of the Aco strain, which is derived from spiny mice of an unidentified species. Although this Aco strain has been tentatively considered A. cahirinus species, it was distinguished from this species based on conventional and G-banding chromosomal patterns of this strain. Subsequently, we determined the nucleotide sequence of the mitochondorial gene cytochrome b (Cyb) and constructed a molecular phylogenetic tree. We found that the Aco strain belongs to the same clade as A. dimidiatus. Based on this molecular analysis, we concluded that the Aco strain of spiny mice should be considered A. dimidiatus. |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 22 |
| End Page | 25 |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Dissecting the hierarchy and lineage of mesenchymal stem cells by mouse genetics |
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| FullText URL | poalas_033_015_017.pdf |
| Author | Takarada, Takeshi| |
| Abstract | Determination of stem cell hierarchy/lineage is indispensable for a better understanding and augmentation of many aspects of medical sciences, including the mechanisms of tissue development and maintenance of tissue homeostasis, as well as disease development. It also has implications in the field of tissue regeneration for medical treatments and disease modeling for drug discovery using iPS technology. Mesenchymal stem cells are multipotent stem cell that can differentiate into various type of cells including osteoblasts, adipocytes, myocytes and chondrocytes. Runt-related transcription factor 2 (Runx2) is an essential transcriptional regulator of osteoblast differentiation. Runx2 deficiency in Prx1+-derived cells (Runx2prx1−/− mice) resulted in defective intramembranous ossification. Double-positive cells for Prx1-GFP and stem cell antigen-1 (Sca1) (Prx1+Sca1+ cells) in the calvaria expressed Runx2 at lower levels and were more homogeneous and primitive as compared with Prx1+Sca1− cells. Our results suggest that osteoblast differentiation in vivo may begin at the Prx1+Sca1+ MSC stage with sequential progression to Prx1+Sca1−cells, then Osx+Prx1−Sca1− osteoblast precursors, which eventually form mature α1(I)-collagen+ osteoblasts. |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 15 |
| End Page | 17 |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Overview and future perspectives of studies on the mechanisms underlying appetite regulation in chickens |
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| FullText URL | poalas_033_008_010.pdf |
| Author | Honda, Kazuhisa| |
| Abstract | Broiler chickens eat more feed than layer chickens. As a result, broiler chickens grow faster than layer chickens. However, excessive accumulation of body fat in broiler chickens has been a serious problem in the poultry industry in recent decades. Therefore, the appetite regulatory system of chickens has been a focus of research among poultry scientists. Lines of evidence suggest that the physiological role of peripheral adiposity hormones, such as leptin and insulin, and gut hormones, such as ghrelin, are different between mammals and chickens. Thus, species specificity make it difficult to understand the mechanisms underlying the regulation of food intake in chickens. Here I provide an overview of recent findings in this field and future perspectives. |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 8 |
| End Page | 10 |
| language | Japanese |
| File Version | publisher |
| Title Alternative | Pharmacokinetic analysis of drug absorption following nasal application to rats |
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| FullText URL | poalas_033_003_007.pdf |
| Author | 古林 呂之| |
| Abstract | Since a drug applied to the nose in an in vivo physiologic condition is translocated to the gastrointestinal (GI) tract by mucociliary clearance (MC), the drug undergoes absorption both from the nasal cavity and from the GI tract. The detailed MC of the rat was examined, using inulin as a marker of the applied solution. Inulin disappeared monoexponentially from the nasal cavity, indicating that the MC can be assumed to follow first-order kinetics. In the proposed kinetic model, the fractional absorption of the drug following nasal application is predicted as the sum of fractional absorption from the nasal cavity and fractional absorption from the GI tract, both of which are estimated indirectly from the permeability to the Caco-2 monolayer. This kinetic model is the first estimation system for nasal drug absorption based on drug disposition after nasal application and is useful for the development of nasal dosage forms. |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 3 |
| End Page | 7 |
| language | Japanese |
| File Version | publisher |
| Title Alternative | A greeting from President of Okayama Association for Laboratory Animal Science |
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| FullText URL | poalas_033_001.pdf |
| Author | Kunieda, Tetsuo| |
| Publication Title | Proceedings of Okayama Association for Laboratory Animal Science |
| Published Date | 2017-04 |
| Volume | volume33 |
| Start Page | 1 |
| End Page | 1 |
| language | Japanese |
| File Version | publisher |
| Author | Kondo, Mariko| Mori, Kazuo| Nomura, Hiroshi| Kadowaki, Hanako| Watanabe, Makiko| Doi, Akemi| Shima, Sayaka| |
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| Published Date | 2016-12-29 |
| Publication Title | Bioethics - Medical, Ethical and Legal Perspectives |
| Content Type | Book |
| FullText URL | K0005564_abstract_review.pdf K0005564_summary.pdf K0005564_fulltext.pdf |
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| Author | Pineda Velasco, Ivan| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5564号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005558_abstract_review.pdf K0005558_fulltext .pdf |
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| Author | Salmie Suhana Binti Che Abdullah| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5558号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005556_abstract_review.pdf K0005556_fulltext.pdf |
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| Author | 郭 嘉躍| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5556号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Engineering |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005555_abstract_review.pdf K0005555_fulltext.pdf |
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| Author | Cagdas, Aksu| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5555号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Engineering |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005548_abstract_review.pdf K0005548_fulltext.pdf |
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| Author | Yin Yin Aye| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5548号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Engineering |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005541_abstract_review.pdf K0005541_fulltext.pdf |
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| Author | Ishikawa, Koji| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5541号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005517_abstract_review.pdf K0005517_fulltext.pdf K0005517_summary.pdf |
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| Author | Matsui, Miyuki| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5517号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Nursing Science |
| Grantor | 岡山大学 |
| language | Japanese |
| FullText URL | K0005510 abstract_review.pdf K0005510_summary.pdf K0005510_fulltext.pdf |
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| Author | Takeuchi, Mayu| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5510号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005509 abstract_review.pdf K0005509_summary.pdf K0005509_fulltext.pdf |
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| Author | Kawabata, Yuya| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5509号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005508 abstract_review.pdf K0005508_summary.pdf K0005508_fulltext.pdf |
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| Author | Kataoka, Kota| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5508号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005500 abstract_review.pdf K0005500_summary.pdf K0005500_fulltext.pdf |
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| Author | Hashimoto, Yuki| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5500号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | English |
| FullText URL | K0005498 abstract_review.pdf K0005498_summary.pdf K0005498_fulltext.pdf K0005498_other.pdf |
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| Author | Tamura, Shohei| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5498号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | Japanese |
| FullText URL | K0005497 abstract_review.pdf K0005497_summary.pdf K0005497_fulltext.pdf |
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| Author | Okada, Ayumi| |
| Published Date | 2017-03-24 |
| Content Type | Thesis or Dissertation |
| Grant Number | 甲第5497号 |
| Granted Date | 2017-03-24 |
| Thesis Type | Doctor of Philosophy in Dental Science |
| Grantor | 岡山大学 |
| language | Japanese |
