| FullText URL | fulltext.pdf |
|---|---|
| Author | Higuchi, Rina| Fujita, Yuri| Namba, Shotaro| Moriya, Hisao| |
| Keywords | yeast overexpression promoter |
| Published Date | 2024-10-18 |
| Publication Title | FEMS Yeast Research |
| Volume | volume24 |
| Publisher | Oxford University Press (OUP) |
| Start Page | foae032 |
| ISSN | 1567-1364 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2024. |
| File Version | publisher |
| PubMed ID | 39424601 |
| DOI | 10.1093/femsyr/foae032 |
| Web of Science KeyUT | 001345875500001 |
| Related Url | isVersionOf https://doi.org/10.1093/femsyr/foae032 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Namba, Shotaro| Moriya, Hisao| |
| Keywords | Aggregation Fluorescent protein Hsp70 Overproduction Toxicity Yeast |
| Published Date | 2024-06-12 |
| Publication Title | Journal of Cell Science |
| Volume | volume137 |
| Issue | issue11 |
| Publisher | The Company of Biologists Ltd |
| Start Page | jcs261977 |
| ISSN | 0021-9533 |
| NCID | AA00694823 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2024. Published by The Company of Biologists Ltd |
| File Version | publisher |
| PubMed ID | 38766715 |
| DOI | 10.1242/jcs.261977 |
| Web of Science KeyUT | 001267257500006 |
| Related Url | isVersionOf https://doi.org/10.1242/jcs.261977 |
| Title Alternative | Evaluator of adaptability of S. cerevisiae to grape juice using the oversxpression profiling ADOPT method |
|---|---|
| FullText URL | srfa_113_001_006.pdf |
| Author | Moriya, Hisao| Ono, Chiyuki Kohata | |
| Abstract | The authors have recently developed the overexpression profiling ADOPT method. In the ADOPT method, yeast strains overexpressing most of the genes in the budding yeast Saccharomyces cerevisiae genome are mixed and competitively cultured, and the genes overexpressed in the enriched strains are systematically identified. Furthermore, the identified genes can be used to identify bottleneck factors that are necessary but lacking for growth of S. cerevisiae under given conditions. In our previous studies, we have identified bottlenecks in artificially created stress environments in the laboratory, but in this study, we used grape juice for winemaking as an example to see if industrial bottlenecks can be identified. ADOPT experiments with sulfite-added grape juice used in conventional winemaking resulted in a strong enrichment of strains overexpressing the sulfite pump SSU1 and its transcription factor FZF1. Since enhancement of SSU1 function is known to occur in wine yeast acclimation, ADOPT was also shown to be useful in the search for industrial bottlenecks. On the other hand, no genes were strongly enriched by ADOPT in grape juice without sulfite addition, suggesting that grape juice is a balanced medium with few bottlenecks for S. cerevisiae growth. |
| Keywords | yeast S. cerevisiae overexpression wine making |
| Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
| Published Date | 2024-02-01 |
| Volume | volume113 |
| Start Page | 1 |
| End Page | 6 |
| ISSN | 2186-7755 |
| language | Japanese |
| File Version | publisher |
| FullText URL | fulltext20230828-02.pdf |
|---|---|
| Author | Ono, Ryota| Saeki, Nozomu| Kojima, Keiichi| Moriya, Hisao| Sudo, Yuki| |
| Keywords | UVA Saccharomyces cerevisiae Iodide Growth inhibition Suppressive molecule |
| Note | © 2023 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/| This fulltext file will be available in Oct. 2024.| |
| Published Date | 2023-10-15 |
| Publication Title | Biochemical and Biophysical Research Communications |
| Volume | volume677 |
| Publisher | Elsevier BV |
| Start Page | 1 |
| End Page | 5 |
| ISSN | 0006-291X |
| NCID | AA00564395 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 Elsevier Inc. |
| File Version | author |
| PubMed ID | 37523893 |
| DOI | 10.1016/j.bbrc.2023.07.048 |
| Web of Science KeyUT | 001047346900001 |
| Related Url | isVersionOf https://doi.org/10.1016/j.bbrc.2023.07.048 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Saeki, Nozomu| Yamamoto, Chie| Eguchi, Yuichi| Sekito, Takayuki| Shigenobu, Shuji| Yoshimura, Mami| Yashiroda, Yoko| Boone, Charles| Moriya, Hisao| |
| Published Date | 2023-04-28 |
| Publication Title | PLoS Genetics |
| Volume | volume19 |
| Issue | issue4 |
| Publisher | Public Library of Science |
| Start Page | e1010732 |
| ISSN | 1553-7404 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2023 Saeki et al. |
| File Version | publisher |
| PubMed ID | 37115757 |
| DOI | 10.1371/journal.pgen.1010732 |
| Web of Science KeyUT | 000978623500001 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pgen.1010732 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Namba, Shotaro| Kato, Hisaaki| Shigenobu, Shuji| Makino, Takashi| Moriya, Hisao| |
| Keywords | yeast fluorescent protein cytotoxicity protein burden heat shock response morphology proteasome |
| Published Date | 2022-04-29 |
| Publication Title | g3－Genes Genomes Genetics |
| Volume | volume12 |
| Issue | issue6 |
| Publisher | Oxford Univ Press Inc. |
| Start Page | jkac106 |
| ISSN | 2160-1836 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2022. |
| File Version | publisher |
| PubMed ID | 35485947 |
| DOI | 10.1093/g3journal/jkac106 |
| Web of Science KeyUT | 000796686500001 |
| Related Url | isVersionOf https://doi.org/10.1093/g3journal/jkac106 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Kintaka, Reiko| Makanae, Koji| Namba, Shotaro| Kato, Hisaaki| Kito, Keiji| Ohnuki, Shinsuke| Ohya, Yoshikazu| Andrews, Brenda J.| Boone, Charles| Moriya, Hisao| |
| Published Date | 2020-11-04 |
| Publication Title | eLife |
| Volume | volume9 |
| Publisher | eLife Sciences Publications |
| Start Page | e54080 |
| ISSN | 2050-084X |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © Kintaka et al. |
| File Version | publisher |
| PubMed ID | 33146608 |
| DOI | 10.7554/eLife.54080 |
| Web of Science KeyUT | 000592848100001 |
| Related Url | isVersionOf https://doi.org/10.7554/eLife.54080 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Ishikawa, Koji| Ishihara, Akari| Moriya, Hisao| |
| Published Date | 2020-10-28 |
| Publication Title | PLoS Genetics |
| Volume | volume16 |
| Issue | issue10 |
| Publisher | Public Library of Science |
| Start Page | e1009091 |
| ISSN | 1553-7404 |
| NCID | AA12073492 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 Ishikawa et al. |
| File Version | publisher |
| PubMed ID | 33112847 |
| DOI | 10.1371/journal.pgen.1009091 |
| Web of Science KeyUT | 000588362000001 |
| Related Url | isVersionOf https://doi.org/10.1371/journal.pgen.1009091 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Mori, Yoshihiro| Yoshida, Yuki| Satoh, Ayano| Moriya, Hisao| |
| Keywords | Biological techniques Cell biology Gene expression analysis Molecular biology Protein translocation Protein transport |
| Published Date | 2020-03-16 |
| Publication Title | Scientific Reports |
| Volume | volume10 |
| Issue | issue1 |
| Publisher | Nature |
| Start Page | 4798 |
| ISSN | 2045-2322 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2020 |
| File Version | publisher |
| PubMed ID | 32179769 |
| DOI | 10.1038/s41598-020-61646-3 |
| Web of Science KeyUT | 000562125100001 |
| Related Url | isVersionOf https://doi.org/10.1038/s41598-020-61646-3 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Saeki, Nozomu| Eguchi, Yuichi| Kintaka, Reiko| Makanae, Koji| Shichino, Yuichi| Iwasaki, Shintaro| Kanno, Manabu| Kimura, Nobutada| Moriya, Hisao| |
| Keywords | Cell growth Gene expression Gene regulation |
| Published Date | 2020-06-11 |
| Publication Title | Scientific Reports |
| Volume | volume10 |
| Issue | issue1 |
| Publisher | Nature |
| Start Page | 9500 |
| ISSN | 2045-2322 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2020 |
| File Version | publisher |
| PubMed ID | 32528012 |
| DOI | 10.1038/s41598-020-66307-z |
| Web of Science KeyUT | 000559960100018 |
| Related Url | isVersionOf https://doi.org/10.1038/s41598-020-66307-z |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Nemoto, Michiko| Iwaki, Sayako| Moriya, Hisao| Monden, Yuki| Tamura, Takashi| Inagaki, Kenji| Mayama, Shigeki| Obuse, Kiori| |
| Keywords | Biomineralization Diatom Silica Transcriptome Proteome |
| Note | This is a post-peer-review, pre-copyedit version of an article published in Marine Biotechnology. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10126-020-09976-1.| |
| Published Date | 2020-06-03 |
| Publication Title | Marine Biotechnology |
| Publisher | Springer |
| ISSN | 1436-2228 |
| NCID | AA11357643 |
| Content Type | Journal Article |
| language | French |
| OAI-PMH Set | 岡山大学 |
| File Version | author |
| PubMed ID | 32488507 |
| DOI | 10.1007/s10126-020-09976-1 |
| Web of Science KeyUT | 000537432000001 |
| Related Url | isVersionOf https://doi.org/10.1007/s10126-020-09976-1 |
| FullText URL | fulltext.pdf |
|---|---|
| Author | Moriya, Hisao| |
| Keywords | green fluorescent protein overexpression expression limit expression level protein cytotoxicity |
| Published Date | 2020-05-14 |
| Publication Title | AIMS Biophysics |
| Volume | volume7 |
| Issue | issue2 |
| Publisher | AIMS Press |
| Start Page | 121 |
| End Page | 132 |
| ISSN | 2377-9098 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © 2020 the Author(s), licensee AIMS Press. |
| File Version | publisher |
| DOI | 10.3934/biophy.2020010 |
| Web of Science KeyUT | 000535774000005 |
| Related Url | isVersionOf https://doi.org/10.3934/biophy.2020010 |
| Title Alternative | Mathematical analysis of copy number variation of 2 μ-based plasmids in yeast cells |
|---|---|
| FullText URL | srfa_109_007_011.pdf |
| Author | Saeki, Nozomu| Moriya, Hisao| |
| Abstract | Plasmids with the 2 μ plasmid origin are commonly-used in the genetic engineering of the budding yeast Saccharomyces cerevisiae. Intracellular copy numbers of 2 μ plasmids are different depending on the genes inserted into the plasmids. This difference is thought to occur from the difference in the growth efficiency （fitness） produced by the positive- and negative-selection biases of genes inserted in the plasmid. In this study, we made a mathematical model based on this assumption. Computational simulations of the model validated that copy numbers of the plasmids are rapidly settled depending on the fitness created by the gene on the plasmid. The copy number of a plasmid only contains a bias to keep the plasmid in a single copy became average 20copies per cell when the plasmid is randomly distributed, suggesting that no positive distribution mechanism is required for a plasmid to become multicopy. |
| Keywords | yeast 2 μ plasmid mathematical model |
| Publication Title | Scientific Reports of the Faculty of Agriculture, Okayama University |
| Published Date | 2020-02-01 |
| Volume | volume109 |
| Start Page | 7 |
| End Page | 11 |
| ISSN | 2186-7755 |
| language | Japanese |
| File Version | publisher |
| FullText URL | SR9_1_8866.pdf |
|---|---|
| Author | Abe-Kanoh, Naomi| Kunisue, Narumi| Myojin, Takumi| Chino, Ayako| Munemasa, Shintaro| Murata, Yoshiyuki| Satoh, Ayano| Moriya, Hisao| Nakamura, Yoshimasa| |
| Published Date | 2019-6-20 |
| Publication Title | Scientific Reports |
| Volume | volume9 |
| Publisher | Nature Publishing Group |
| Start Page | 8866 |
| ISSN | 2045-2322 |
| Content Type | Journal Article |
| language | English |
| OAI-PMH Set | 岡山大学 |
| Copyright Holders | © The Author(s) 2019 |
| File Version | publisher |
| PubMed ID | 31222108 |
| DOI | 10.1038/s41598-019-45248-2 |
| Web of Science KeyUT | 000472136900040 |
| Related Url | isVersionOf https://doi.org/10.1038/s41598-019-45248-2 |
