1. The absorption spectra of urobilin, bilirubin, uroerythrin, urochrom A and urochrom B were determined after separating them from urine. 2. In the absorption spectra using some solvents, there were other absorption spectra which were specific to the solvents and unspecific to the solutes. The mechanism of these absorption spectra was clarified. 3. Besides urochrom, uroerythrin had the most contributing factor to the color of urine, on the other hand urobilin and bilirubin were displaying very little rolls.