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ID 32955
フルテキストURL
著者
Izumi, Yohei Okayama University
Sonoda, Shoji Okayama University
Danksa, Hugh V Canadian Museum of Nature, Ottawa, Canada
Tsumuki, Hisaaki Okayama University
抄録

Overwintering larvae of the rice stem borer, Chilo suppressalis accumulate glycerol and are freezing tolerant to about -25°C. However, non-diapausing larvae cannot accumulate glycerol and are killed by freezing. We compared the extent of tissue damage, the effects of glycerol concentration, and the transport of glycerol and water in fat body tissues from these larvae at selected freezing temperatures. Tissues from overwintering larvae, but not non-diapausing larvae, survive when frozen at -20°C with 0.25M glycerol, but the protection afforded by glycerol is offset by the water-channel inhibitor mercuric chloride. Glycerol in higher concentration (0.75M) affords some protection even to the fat body of non-diapausing larvae. Radiotracer assays of overwintering larvae show that water leaves the tissues during freezing while glycerol enters, and that mercuric chloride disrupts this process. Transport is also disrupted after lethal freezing at -35°C. Therefore, membrane transport of water and glycerol is involved in the avoidance of freezing injury to fat body cells of the rice stem borer, apparently by mediating the replacement of water with glycerol in freezing-tolerant tissues.

キーワード
Freeze tolerance
Glycerol
Water
Membrane transport
Chilo suppressalis
備考
Digital Object Identifier:10.1016/j.jinsphys.2005.11.001
Published with permission from the copyright holder. This is the institute's copy, as published in Journal of Insect Physiology, February 2006, Volume 52, Issue 2, Pages 215-220.
Publisher URL:http://dx.doi.org/10.1016/j.jinsphys.2005.11.001
Direct access to Thomson Web of Science record
Copyright © 2005 Elsevier B. V. All rights reserved.
発行日
2006-2
出版物タイトル
Journal of Insect Physiology
52巻
2号
開始ページ
215
終了ページ
220
資料タイプ
学術雑誌論文
言語
English
査読
有り
DOI
Web of Science KeyUT
Submission Path
zoology_general/1