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ID 32955
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Author
Izumi, Yohei
Danksa, Hugh V
Tsumuki, Hisaaki
Abstract

Overwintering larvae of the rice stem borer, Chilo suppressalis accumulate glycerol and are freezing tolerant to about -25°C. However, non-diapausing larvae cannot accumulate glycerol and are killed by freezing. We compared the extent of tissue damage, the effects of glycerol concentration, and the transport of glycerol and water in fat body tissues from these larvae at selected freezing temperatures. Tissues from overwintering larvae, but not non-diapausing larvae, survive when frozen at -20°C with 0.25M glycerol, but the protection afforded by glycerol is offset by the water-channel inhibitor mercuric chloride. Glycerol in higher concentration (0.75M) affords some protection even to the fat body of non-diapausing larvae. Radiotracer assays of overwintering larvae show that water leaves the tissues during freezing while glycerol enters, and that mercuric chloride disrupts this process. Transport is also disrupted after lethal freezing at -35°C. Therefore, membrane transport of water and glycerol is involved in the avoidance of freezing injury to fat body cells of the rice stem borer, apparently by mediating the replacement of water with glycerol in freezing-tolerant tissues.

Keywords
Freeze tolerance
Glycerol
Water
Membrane transport
Chilo suppressalis
Note
Digital Object Identifier:10.1016/j.jinsphys.2005.11.001
Published with permission from the copyright holder. This is the institute's copy, as published in Journal of Insect Physiology, February 2006, Volume 52, Issue 2, Pages 215-220.
Publisher URL:http://dx.doi.org/10.1016/j.jinsphys.2005.11.001
Direct access to Thomson Web of Science record
Copyright © 2005 Elsevier B. V. All rights reserved.
Published Date
2006-2
Publication Title
Journal of Insect Physiology
Volume
volume52
Issue
issue2
Start Page
215
End Page
220
Content Type
Journal Article
language
英語
Refereed
True
DOI
Web of Science KeyUT
Submission Path
zoology_general/1