ID | 48822 |
FullText URL | |
Author |
Zong, Min
Yu, Mei Kuen
Siu, Ka Yu
Ng, Wing Yan
Chan, Hsiao Chang
Tanner, Julian A.
Yu, Sidney
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Abstract | Background: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediate
compartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150Glued, a subunit of dynactin, has
been implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However,
whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPII
vesicles and microtubules is currently unknown.
Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPP
subunits interfered with microtubule architecture by competing p150Glued away from the MTOC. TRAPP subunit TRAPPC9
bound directly to p150Glued via the same carboxyl terminal domain of p150Glued that binds Sec23 and Sec24. TRAPPC9 also
inhibited the interaction between p150Glued and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves to
uncouple p150Glued from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane.
Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGIC
membrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPP
allows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi.
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Note | 記事番号:e29995
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Published Date | 2012-01-18
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Publication Title |
PLoS ONE
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Volume | volume7
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Issue | issue1
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ISSN | 1932-6203
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Content Type |
Journal Article
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Official Url | http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0029995#top
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language |
English
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Copyright Holders | © 2012 Zong et al.
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File Version | publisher
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Refereed |
True
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DOI | |
PubMed ID | |
Web of Science KeyUT | |
Pages | 12
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