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ID 12078
Eprint ID
12078
FullText URL
Thumnail K003365.pdf 251 KB
Title Alternative
軟骨細胞におけるLRP1,CCN2受容体の機能の可能性
Author
Kawata, Kazumi
Abstract
Low density lipoprotein receptor (LDLR)-related protein 1 (LRP1/CD91) is one of the receptors of CCN2 that conducts endochondral ossification and cartilage repair. LRP1 is a well-known endocytic receptor, but its distribution among chondrocytes remains to be elucidated. We herein demonstrate for the first time that the distribution of LRP1 in chondrocytes except for hypertrophic chondrocytes in vivo and in vitro. Interestingly, the LRP1 levels were higher in mature chondrocytic HCS-2/8 and osteoblastic SaOS-2 than in other cells, whereas the other LDLR family members involved in ossification were detected at lower levels in HCS-2/8. It was interesting to note that in HCS-2/8, LRP1 was observed not only on the cell surface and in the cytoplasm, but also in the nucleus. Exogenously added CCN2 was incorporated into HCS-2/8, which was partially co-localized with LRP1, and targeted to the recycling endosomes and nucleus as well as the lysosomes. These findings suggest specific roles of LRP1 in cartilage biology.
Keywords
Low density lipoprotein receptor-related protein 1 (LRP1)
Cartilage
Chondrocyte differentiation
CCN2/connective tissue growth factor (CTGF)
Endocytosis
Note
http://dx.doi.org/10.1016/j.bbrc.2006.04.109
Published Date
2006-03-24
Publication Title
Content Type
Thesis or Dissertation
Grant Number
甲第3365号
Granted Date
2006-03-24
Thesis Type
博士(歯学)
Grantor
岡山大学
language
日本語
File Version
none
Refereed
Unknown