Journal of Okayama Medical Association
Published by Okayama Medical Association

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SV40小型腫瘍抗原遺伝子のクローン化と抗原の精製

Ikeda, Shogo
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Abstract
The simian virus 40 (SV40) DNA fragment encoding small t antigen was cloned with expression vector pUC8 for the purification of small t-antigen. Small t antigen produced in Escherichia coli was a hybrid protein of 22,000 daltons which comprised about 6% of the total protein. Hybrid small t antigen maintained antigenic activity which was examined by Western-blotting and enzyme-linked immunoassay. Hybrid small t antigen was extracted from E. coli with urea and purified from a small t-antigen band in SDS-polyacrylamide gel. The purified antigen maintained antigenic activity.
Keywords
SV40 t 抗原
遺伝子のクローン化
発現ベクター
ウエスタン・ブロッティング
ISSN
0030-1558
NCID
AN00032489