| JaLCDOI |
10.18926/AMO/45270
|
| フルテキストURL |
65_2_113.pdf
|
| 著者 |
Morikawa, Toshio|
Yamamoto, Yuji|
Miyaishi, Satoru|
|
| 抄録 |
We have developed a new method for sex determination based on simultaneous detection of the SRY (sex-determining region Y), STS (steroid sulfatase) and amelogenin (AMELX and AMELY) gene regions and their homologous sequences. The sex of 246 blood samples was correctly determined by this method. An AMELY-deleted male sample, which would have been erroneously considered female based solely on analysis of the amelogenin locus, was successfully identified as male by the present method. The detection limit of this method was 63 pg of genomic DNA, and the male DNA component could be detected from mixed samples having a male:female ratio as low as 1:10. This method was useful for degraded DNA and possessed the human specificity. Practical application to 35 autopsy cases is described.
|
| キーワード |
sex determination
SRY (sex-determining region Y)
multiplex PCR
forensic casework
|
| Amo Type |
Original Article
|
| 出版物タイトル |
Acta Medica Okayama
|
| 発行日 |
2011-04
|
| 巻 |
65巻
|
| 号 |
2号
|
| 出版者 |
Okayama University Medical School
|
| 開始ページ |
113
|
| 終了ページ |
122
|
| ISSN |
0386-300X
|
| NCID |
AA00508441
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
英語
|
| 著作権者 |
CopyrightⒸ 2011 by Okayama University Medical School
|
| 論文のバージョン |
publisher
|
| 査読 |
有り
|
| PubMed ID |
21519369
|
| Web of Science KeyUT |
000289818800007
|
