検索結果 21698 件
| タイトル（別表記） | Sources of Resistance to Net Blotch in Barley Germplasm |
|---|---|
| フルテキストURL | 002_001_091_102.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
| キーワード | Barley Net blotch Disease resistance Genetic resources Race differentiation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 91 |
| 終了ページ | 102 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313965 |
| タイトル（別表記） | Diallel Analysis for the Percentage of Grains with Hull Rupture in F2 Populations of Two-rowed Barley |
|---|---|
| フルテキストURL | 002_001_079_089.pdf |
| 著者 | 呉 基日| 武田 和義| 金谷 良一| |
| 抄録 | Half diallel F2 populations crossed among eight two-rowed barley varieties with warious percentages of hull ruptured grains were raised to analyze the inheritance of the trait. The percentage of grains with hull rupture varied from 0 to 87% among the parents. The diallel analysis revealed the following: the trait was predominantly controlled by the additive genes, and the dominance effect of some parents was also significant. However, the epistatic effect of the genes was not significant. The average dominance was 0.97. The heritability value was estimated as 0.57 and 0.91 in a narrow and a broad sense, respectivelly. The percentage of grains with hull rupture showed continuous and transgressive segregations in 28 F2 populations derived from half diallel crosses among eight parents. Heritability of the trait in a broad sense was 0.43~0.80(0.65 on average) in 28 F2 populations. |
| キーワード | Barley Grain quality Hull rupture Diallel analysis Heritability |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 79 |
| 終了ページ | 89 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Character Expression and Inheritance of a "Short Upper Leaves" Mutant in Barley |
|---|---|
| フルテキストURL | 002_001_069_078.pdf |
| 著者 | 金谷 良一| 呉 基日| 武田 和義| |
| 抄録 | A mutant which devolops an extremely shortened flag leaf and shorter upper leaves was obtained from the progeny of 90 KR of gamma-ray irradiated Fuji Nijo, a malting barley variety. This mutant was controlled by a single partial dominant gene named Sul (short upper leaves). The character expression and the inheritance of the gene were investigated in this study. Sul shortened the flag leaf and upper several leaves and uppermost internode length, while it did not affect the length of spike, lower leaves and the second or lower internodes. A reciprocal translocation of the chromosomes was observed in this mutant line, and the Sull gene was linked with the V (two-rowed) gene on chromosome 2, and also linked with the breaking point of the reciprocal translocation. Small leaf area caused by the Sull gene might reduce the mutual shading and the transpiration of the leaf canopy at the later growth stages. |
| キーワード | Barley Plant type Linkage analysis Developmental pattern |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 69 |
| 終了ページ | 78 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Genetic Analysis of Large Trichome in Barley Leaf Blade |
|---|---|
| フルテキストURL | 002_001_063_068.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | The inheritance and linkage relationship of a new hairiness trait "large trichome" was investigated in barley. Although the size of large trichome is about four times that of normal one, the character can not be recognized with the naked eye. However, it is easily identified by the roughness of leaf touch. The large trichomes develop on both sides of the leaf blades. The direction of trichome is both acropetal and basipetal. It is clearly distinguished from the extremely long trichome controlled by Pub gene. About 2,300 varieties of our Barley Germplasm Center were screened by the leaf touch to find nine varieties with large trichome. Two of them were six-rowed local variety from Pakistan, and other seven were two-rowed varieties from Europe and Japan. All of them were hulled type. Crosses of six large trichome varieties with a normal Japanese variety resulted in the large trichome type F1s, suggesting the dominant nature of the trait. The large trichome line Hokuiku 17 was crossed with various linkage testres to study the mode of inheritance and the linkage relationship of the gene. In the F2 populations, the large trichome was controlled by a single dominant gene named Ltr (large trichome), which was independentiy inherited from the following marker genes; br and gl-5 on chromosome 1; li and ν on chromosome 2; uz on chromosome 3; K and gl-3 on chromosome 4; trd on chromosome 5; ο on chromosome 6. On the other hand, from the cross between Hokuiku 17 and OUL166, Ltr was found to be linked with s and fs on chromosome 7. Although the allelism test has not been completed, the very low frequency of the large trichome type (9/2,300) indicates that the variant resulted from a recent mutation event, or the fitness of the variant is low in the natural and/or artificial selection. |
| キーワード | Barley Trichome Linkage analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 63 |
| 終了ページ | 68 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313663 |
| タイトル（別表記） | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
|---|---|
| フルテキストURL | 003_002_117_127.pdf |
| 著者 | 米谷 力| 森次 益三| 河﨑 利夫| |
| 抄録 | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
| キーワード | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 2号 |
| 開始ページ | 117 |
| 終了ページ | 127 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Inheritance of Phenol Reaction in the Awn of Barley |
|---|---|
| フルテキストURL | 002_001_055_062.pdf |
| 著者 | 武田 和義| 張 成林| 金谷 良市| |
| 抄録 | The inheritance and geographical distribution of the phenol reaction in rice has been investigated intensively. On the other hand, in the case of barley, almost all of the varieties show positive reaction to phenol, and inheritance study of the trait has not yet been reported. We investigated the phenol reaction of ca. 5,000 barley varieties preserved at the Barley Germplasm Center of Okayama University to find about 30 varieties which show a negative reaction to the phenol. These varieties are mainly from Southwest Asia. The reaction of awn was sharpest at all parts of the plant including seeds. The positive reaction must be the prototype of barley, because Hordeum spontaneum, a possible ancestor of the cultivated barley, shows positive reaction. Inheritance and linkage studies were conducted using varieties with negative reaction and linkage testers with positive reaction. Phenol reaction was dominant and showed a clear 3:1 segregation ratio in the F2 populations, indicating the trait was controlled by a single dominant gene Ph(phenol reaction). Linkage study revealed that Ph was linked with four marker genes(three loci) on the chromosome 2 and independent of 13 other marker genes located on the chromosomes except chromosome 2. Ph may be a useful marker gene and an interesting material for molecular-biological studies. |
| キーワード | Barley Phenol reaction Linkage analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 55 |
| 終了ページ | 62 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Genetical Studies on Callus Growth and Plant Regeneration from Immature Embryos in Barley |
|---|---|
| フルテキストURL | 002_001_043_053.pdf |
| 著者 | 間野 吉郎| 力石 和英| 安田 昭三| |
| 抄録 | Immature embryos of 99 varieties of barley were cultured to investigate the ability of callus growth and plant regeneration. These two in vitro traits showed wide and continuous variations among the barley varieties tested. Ability of callus growth, which were evaluated by callus diameter ranged from 3.9mm to 11.2mm, and ability of plant regeneration from the calli ranged from 0% to 100%. A set of complete diallel crosses was made using six cultivars as the parents which differed in ability of callus growth and plant regeneration. The Vr/Wr graphical analysis showed that there were epistasis, or interaction among nonallelic genes for callus growth. As to ability of plant regeneration, no epistasis existed in the subdiallel without P1 (J232) which showed high specific combining ability, and it was controlled by a simple additive dominance genetic system. The mean degree of dominance(0.42) was relatively low and the broad(0.86) and narrow(0.78) sense heritabilities were high. |
| キーワード | Barley Tissue culture Plant regeneration Diallel analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 43 |
| 終了ページ | 53 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
|---|---|
| フルテキストURL | 003_002_091_103.pdf |
| 著者 | 森次 益三| 河﨑 利夫| 鈴木 孝夫| |
| 抄録 | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
| キーワード | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 2号 |
| 開始ページ | 091 |
| 終了ページ | 103 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Comparison of Regenerating Ability of Calli Derived from Mature and Immature Embryos in Barley Varieties |
|---|---|
| フルテキストURL | 002_001_033_042.pdf |
| 著者 | 力石 和英| 安田 昭三| |
| 抄録 | The callus forming ability and regenerating ability of the calli derived from mature and immature embryos of 132 barley varieties were examined. These materials were taken from a world-wide collection preserved at the Barley Germplasm Center of Okayama University. The callus forming ability varied widely according to genotype in both mature and immature embryos, but the varieties collected from Ethiopia showed low callus forming ability. Calli derived from mature embryos generally did not regenerate shoots, except for three Japanese varieties. The frequency of shoot regeneration from the calli derived from immature embryos was somewhat higher than that from those derived from mature embryos. Many of the Korean and Japanese varieties had a high shoot regenerating ability. However, few of the varieties from Ethiopia and Southwest Asia had a high shoot regenerating ability. No correlation was observed btween root regenerating ability and shoot regenerating ability of the varieties. No correlation was observed between callus proliferation and root regenerating ability between calli derived from mature and immature embryos. We could not find any difference in the shoot regenerating ability btween the two-rowed and six-rowed genotypes. |
| キーワード | Barley Tissue culture Mature embryo Immature embryo Regenerating |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 33 |
| 終了ページ | 42 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Promotion of Drying of Leaves Detached from Plants after Rainfall Exposure |
|---|---|
| フルテキストURL | 002_002_191_200.pdf |
| 著者 | 木村 和義| |
| 抄録 | The drying of leaves detached from 16 plants (mainly, vegetalbles) after artificial rainfall (mist)treatment was examined in a growth chamber(20℃,8 klux). Leaves detached from misted plants had a higher drying rate than those detached from non-misted plants. The promoting effect of mist on the dryint rate was increased with the increase in the duration of mist exposure. The degree of drying after mist exposure for 3 days or more was increased markedly. In almost all the plants,the weight of the leaves detached from the plants decreased to 10-20% of the initial weight 24 hours after a 5-day mist treatment, but 40-50% in cabbadge, chinese leek and welsh onion. In general, the degree of drying was greater in the young developing leaves or older leaves than in active young leaves. These findings suggested that the surface wax and cuticular of the leaf is injured by rainfall wetting, and that water discharge(transpitation) in the leaf is increased greatly. |
| キーワード | Rainfall Leaf Drying Vegetables |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 2号 |
| 開始ページ | 191 |
| 終了ページ | 200 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Varietal Variation of Earliness in a Narrow Sense and Its Significance for Adaptation in Barley |
|---|---|
| フルテキストURL | 002_001_023_032.pdf |
| 著者 | 大久保 和男| 安田 昭三| 武田 和義| |
| 抄録 | Heading time of is a complex character, which is controlled by several internal factors, namely, vernalization requirement, photoperiodic response and earliness in a narrow sense (ENS). The ecological significance of vernalization requirement and photoperiodic response has been clarified. However, the significance of ENS is not explained well. In this study, we examined the varietal variation of ENS, its geographical distribution and significance of ENS for regional adaptability in barley. The ENS was estimated from the number of days from sowing to flag-leaf emergence of sufficiently vernalized plants grown under a 24-h. photoperiod at 20℃ condition. The variation of ENS was determined from the number of leaves and leaf emergence intervals under the controlled condition. The ENS of 1,017 barley varieties ranged from 12 to 28 days. ENS was shortest in varieties from low-latitudes, while it was longer in varieties from high-latitudes and very low-latitudes. Multiple regression analysis for heading time(Y) on the ENS(X1) and the photoperiodic response(X2) was carried out using various data obtained from different experiment sites and sowing times. The multiple correlation was highly significant in all cases. It was clear that the ENS played an important role for determining heading time in the spring-sown conditions, while the photoperiodic response did in the fall-sown condition. |
| キーワード | Barley Heading time Geographica distribution Adaptability |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 23 |
| 終了ページ | 32 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Studies on Heterosis in Barley Cultivars |
|---|---|
| フルテキストURL | 002_001_007_022.pdf |
| 著者 | 安田 昭三| 林 二郎| 守屋 勇| |
| 抄録 | In cultivated barley, various hybrid injuries such as brittle rachis and hybrid weakness occur. Therefore, crossing must be done among cultivars to prevent the occurrence of such hybrid injuries. In the present study, we examined the magnitude of the heterosis in the crossings and geographical distribution of barley cultivars concerning heterosis. A few Japanese cultivars were used as the common parent for crossing. (1) In the six-rowed barley, 43 cultivars collected from Japan, China and Korea were crossed with non-uzu covered and naked cultivars, Shiroyo-shigara 2 and Nami-Akashinriki, respectively. The largest magnitude of heterosis in grain yield per plant was obtained in the crossing between the Korean cultivar and one of the Japanese common parents. (2) Heterosis in two-rowed cultivars was investigated using a total of 156 F1S which were cross combinations of cultivars collected from the world with two common male parents, Amagi Nijo and Kawasaigoku. The heterosis was larger larger in the cross combinations with Turkish and Ethiopian cultivars. The largest F1/BP was 1.66 in Turkish and 1.45 in Ethiopian cultivars. (3) As to uzu or semi-dwarf cultivars peculiar to Japan, 34 cultivars were crossed with two common male parents, Sekitori (covered) and Akashinriki (naked). Expression of the heterosis was rather conspicuous in the crosses of covered×naked cultivars than those of covered×covered and naked×naked ones. (4) Half diallel analysis using 10 two-rowed cultivars showed that one Ethiopian cultivar expressed a high grade of general combining ability and that one French cultivar was of higher specific combining ability. (5) The most effective agronomic character of the two-rowed cultivars to the grain yield per piant was number of ears in an ordinary year, while in the wet year it was 1000 grain weight. This was considered to be due to overluxuriant growth accompanied by lowering of the percentage of ripened grain. However, in some cultivars the percentage of ripened grain was not lowered under the overluxuriant growth condition. |
| キーワード | Barley Heterosis Diallel analysis Overluxuriant growth Geographical variety |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 7 |
| 終了ページ | 22 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Establishment of a Seedling Test for Resistance to Net Blotch in Barley and a Search for Resistant Varieties |
|---|---|
| フルテキストURL | 001_001_075_090.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | 大麦網斑病は糸状菌の1種であるPyrenophora teres Drechs.の感染によって葉身、葉鞘等に網目状の病斑を生じ、子実の登熟低下によって減収する共に、ビールオオムギにおいては醸造品質であるエキス分が低下する重要病害である。本病害は世界各地のオオムギ栽培地帯のうち主として温暖・湿潤な地域に分布しており（Shipton et al.1973）、近年、連作や灌漑によって被害が増大しつつある（Mathre 1982）。我国においては従来からその存在が確認されていたものの、登熟後期の活性の衰えた葉に生じる病害として重要性は認識されていなかった。しかし、最近、北海道、鳥取県、鹿児島県などのビールオオムギ栽培地帯で局所的な激発事例が確認されている。（佐藤、未発表）。本病害に対する防除法としては種子消毒ならびに殺菌剤の茎葉撒布が有効であるが、その効果は完全ではない。また、茎葉撒布はコストが高く、環境汚染の問題もあるので、最も有効で経済的かつ安全な防除法は抵抗性品種を栽培することと言っても良い。従来、本病害の積極的な抵抗性育種は行われていなかったが、最近は抵抗性を有する品種も育成されている（Metcalfe 1987）。抵抗性品種を育成するためには、遺伝資源ならびに雑種後代を効率よく評価、選抜するための検定方法を確立しなければならない。本病抵抗性の検定方法としては幼苗検定法、圃場検定法が考案されて広く用いられているが（Buchannon and McDonald 1965, Holtmeyer and Webster 1981）、環境条件の変化によって抵抗性が変動する事例が報告されているので（Khan and Boyd 1970, Tekauz 1986）、抵抗性を確実に評価するための安定した検定条件を設定する必要がある。抵抗性に関する遺伝資源についてはSchaller and Wiebe （1952）、Dessouki et al.（1965）およびBuchannon and McDonald （1965）等がそれぞれ数千品種を評価し、中国東北部、トルコおよびエチオピアなどに抵抗性の遺伝資源が豊富であること報告している。それらの品種のいくつかについては、抵抗性の遺伝子分析が行われており（Bockelman et al. 1977, Davis et al. 1990）、本病抵抗性育種の交配親として使用されている（Tekazu and Buchannon 1977, Moseman and Smith 1985）。岡山大学資源生物科学研究所大麦系統保存施設は世界的にも貴重なと東アジアの遺伝資源をはじめ五千余の保存品種を有するが、著者らは大麦網斑病の幼苗検定法を確立し、これらの品種の抵抗性を評価したので報告する。 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 75 |
| 終了ページ | 90 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313461 |
| タイトル（別表記） | Changes in Mitochondrial Spectra of Submerged Rice Seedlings after Exposure to Air Gaussian Deconvolution Analysis |
|---|---|
| フルテキストURL | 002_002_135_147.pdf |
| 著者 | 柴坂 三根夫| 辻 英夫| |
| 抄録 | Quantitative changes in mitochondrial cytochromes of submerged rice seedlings after exposure to air were investigated using gaussian deconvolution analysis on absorption spectrum at liquid nitrogen temperature. Differnece spectra were obtained from subtracting the absorption spectra of oxidized mitochondria from reduced mitochondria by succinate with antimycin-A. they showed three kinds of b-type cytochrome and a spectral component similar to cytochrome c1. Subtraction of the cytochrome c1-like component from the spectrum by curve analysis showed that three cytochrome bs had similar absorption maximum and that on a mitochondrial protein basis they were constant during air-adaptation. Cytochrome c per mitochontorial protein in submerged seedlings was about half of that in aerobic seedlings and increased by 1.5-fold during air-adaptation. The change in cytochrome c content was in paralleled with that of cytochrome aa3. These findings revealed that there were two groups of cytochromes in the response to air-adaptation,i.e.,three cytochrome bs were constant and cytochoromes c and aa3 increased in parallel. |
| キーワード | Absorption spectrum Air-adaptation Cytocrome Gaussian deconvolution analysis Oryza sativa |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 2号 |
| 開始ページ | 135 |
| 終了ページ | 147 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Highly Sensitive Detection of Cucumber Mosaic Virus by Using Fluorogenic Substrate or Enzyme-Amplified Assay in Enzyme-Linked Immunosorbent Assay |
|---|---|
| フルテキストURL | 001_002_167_174.pdf |
| 著者 | 前田 孚憲| 佐古 宣道| 井上 成信| |
| 抄録 | Monoclonal antibody (MAb) was applied to a double-antibody sandwich ELISA (DAS ELISA) for highly sensitive detection of cucumber mosaic virus. Alkaline phosphatase-labeled MAb with high activity was used to raise the ratio of spocific-and nonspecific-adsorption of conjugate (S/N ratio) on polyclonal antibody coated solid phase. Also the assay with a fluorogenic substrace or an enzyme-amplified assay which is highly sensitive for measurment of activity was employed to increase the sensitivity of the assays. This ELISA systems proved to be more sensitive than the conventional assay using ρ-nitorophenyl phosphate, and could detect 100 pg/ml of purified virus. |
| キーワード | Cucumber mosaic virus Monoclonal antibody DAS ELISA Fluorogenic substrate Enzyme-amplified assay |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 167 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Regional Specialities in Ecological Agronomic Characters of Two-rowed Barley Cultivers from Japan and Other Countries |
|---|---|
| フルテキストURL | 001_001_061_074.pdf |
| 著者 | 安田 昭三| 林 二郎| 守屋 勇| |
| 抄録 | 我が国では、戦前から戦後にかけてオオムギ栽培が盛んに行われたが六条オオムギが主であった。しかし、昭和30年代になると醸造用あるいは飼料用として二条オオムギの栽培が盛んになり、最近では従来の六条オオムギから二条オオムギにほとんど置き替わっている。我が国における二条オオムギは明治以後欧州あるいは米国から導入された品種が、選抜を受けたり交配母本に用いられて定着したもので、我が国在来の六条オオムギにくらべて歴史は新しく（高橋ら1981）。これらの二条品種は日本の風土に適するように改良が加えられていることはもちろんであるが、それにしても、二条性そのものが我が国にはなかった形質であり、春播性、ウドンコ病抵抗性、短毛底刺などいわゆる外来の特徴を併せ持っている。このような外来の形質そのものがどの程度我が国の環境条件の中で生かされ、いわゆる日本化が進んでいるかは、今後の二条オオムギ育種上極めて有用な知見になるものと思われる。そのためには日本で栽培されている二条品種と、外国の二条オオムギの主な分布地域から収集した品種とを比較対照して、生理、遺伝的解析を進める必要がある。本研究はこのような意図の下に行われた。以下に結果の概要を示す。 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 61 |
| 終了ページ | 74 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 001_002_159_166.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| キーワード | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 159 |
| 終了ページ | 166 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 高対塩性オオムギ品種の選抜 |
|---|---|
| フルテキストURL | 001_002_113_129.pdf |
| 著者 | Sopandie Didy| 武田 和義| 森次 益三| 川﨑 利夫| |
| 抄録 | In order to select higher salt tolerant cultivars of barley,solution culture experiments were carried out with 500 barley cultivars, which were preliminarily selected from 4581 cultivars by exposure to 1-2% of sodium chloride(MaCl) during the germinating stage. The concentrations of NaCl used in the experiments were 2 (control), 150 and 300 mM in nutrient solution.From the results of five solution culture experiments, each of which was carried out with about 100 cultivars, 105 cultivars of barley were selected as relatively about 100 cultivars. Then the final selection was carried out on those 105 cultivars, and it was found that more than 10 cultivars were highly salt tolerant, mainly based on the comparisons of shoot yields under the supply of 300 mM of NaCl.High salt tolerant barley cultivars mentioned above showed 42-86% and 17-30% of the control in shoot yield under the condition of 150 and 300 mM of NaCl, respectively.On three groups of barley,each having more than 10 cultivars, which are high, moderate and low in salt tolerance, ion contents of the plants were analyzed.The results suggest that potassium(K) uptake was comparatively larger in the high salt tolerant goup of barley cultivars,through there was a big variation in sodium(Na) and K contents even within the same group. |
| キーワード | Barley Ion content Salt tolerance Sodium chloride Yield |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 113 |
| 終了ページ | 129 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Hybrid Progenies Virulent to Wheat and Agropyron Derived from a Cross Between Barley Mildew Isolates (Erysiphe graminis DC. f. sp. hordei) |
|---|---|
| フルテキストURL | 001_001_051_059.pdf |
| 著者 | 部田 英雄| 武田 和義| |
| 抄録 | イネ科植物うどんこ病菌（Erysiphe graminis DC.）はコムギ、オオムギ、カモジグサ、ライムギなど多くのイネ科植物に寄生するが、それぞれの菌は宿主特異性によっていくつかのformae specialesに分化しており、例えば、オオムギうどんこ病菌はオオムギ属以外の植物には感染しないといわている。しかしながら、Cherewickはイネ科植物うどんこ病菌について、元々の宿主とは異なる植物に接触する交互接種を試み、カモジグから分離したうどんこ病菌がコムギを犯す事例を見出した。またHardisonはいろいろなイネ科植物から分離したうどんこ病菌を用いて多くの交互接種を行った結果、ほとんどの菌株は2つあるいはそれ以上の属の植物に病原性を持っていることを認めた。さらに、日浦および日浦と部田はコムギうどんこ病菌とカモジグサうどんこ病菌の種間交雑によって得られた後代から、コムギあるいはカモジグサのいずれかに病原性を持つ菌株はもちろん、コムギとカモジグサの両方に病原性を持つ新しい菌株も容易に作れることを報告した。一方、これらの分化型の中にはそれぞれの宿主の品種あるいは系統に対して病原性の異なる多くのphysiologic raceが分化しており、それらの宿主-病原関係には、いわゆるgene for gene仮設が適合すると考えられている。しかしながら、部田はオオムギうどんこ病菌抵抗性遺伝子JMlsnに対して病原性のない菌株h9とH14の交雑後代からJMlsnに病原性のある菌株を作出した。これらの事実はうどんこ病における宿主-病原菌の相互作用が単純な主働遺伝子によるgene for gene仮設では説明し切れないことを示しており、このような非病原性菌株間の交雑後代から病原性のある菌株が脈生する遺伝的な機構を解明することは、うどんこ病抵抗性育種を進める上でも極めて重要であると思われる。 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 51 |
| 終了ページ | 59 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 日本とカナダの春播オオムギから採取した大麦網斑病菌株の病原性の変異 |
|---|---|
| フルテキストURL | 001_002_147_158.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | Twenty-two isolates of Pyrenophora teres Drechs. collected from Japanese and Canadian spring barleys were inoculated to 38 barley varieties having various genetic backgrounds. The analysis of variance for the discase ratings showed that there were significant differences both in the virulence of isolates and the resistance of varieties. However, the interaction among isolates and varieties was not statistically significant. Both Finlay-Wilkinson regression analysis and principal component analysis by Additive Main effects and Multiplicative Interaction effects(AMMI)model classified the isolates into three groups,which were different in origins and sympton types. A spot tyte isolate was distinguished from net type isolates by its generally high virulence. A slight pathogenic differentiation was suggested between Japanese and Canadian net type isolates. |
| キーワード | Hordeum vulgare Pyrenophora teres Barley Net blotch Race differentiation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 147 |
| 終了ページ | 158 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313862 |