各種動物の骨髄体外組織培養 第3編 骨髄細胞の生体染色に就て 附,全編の総括

With bone-marrow tissue culture of various animals, namely, mammals (mice, albino rats, guinea pigs, cats and dogs), birds (chicken and pigeons), and cold blooded animals (frogs), the author studied neutral red vital staining of bone marrow cells and arrived at the following conclusions. 1) In the neutrophilic series, granules of young immature cells have a strong affinity to neutral red, and mature neutrophils are stained in intermediate degree. The stained granules tend to fuse with one another and grow larger alog with the lapse of time. 2) Practically all eosinophils are stained light red, while basophilocytes are all stained deep red. Eosinophils of birds show some reluctancy to be stained. Monocytes on the whole are stained in intermediate degree and they are usually arranged in the rosette formation, and those in birds and frogs are arranged in a typical fashion, Lymphocytes, megakaryocytss thrombocytes and spindle cells all clearly show neutral red granules. 3) As for the stainability of neutrophils and pseudo-eosinophils with respect to the passing of time in birds, it is generally highest in pseudo-eosinophils and also the average rate of staining is higher than that of mammals, but their color fades more quickly. In neutrophils of frogs the rate of this color-fading is rather slower than that of warm-blooded animals.