検索結果 1906 件
| タイトル（別表記） | ジゴキシゲニン標識プローブを用いたBeet Necrotic Yellow Vein Virus RNA の検出 |
|---|---|
| フルテキストURL | 005_001_079_096.pdf |
| 著者 | 齊藤 美奈子| 木口 忠彦| 玉田 哲男| |
| 抄録 | Complementary DNA (cDNA) clones corresponding to each of five distinct RNA species of beet necrotic yellow vein (BNYVV) were synthesized and identified. The sizes of each cDNA clone for RNAs 1,2,3,4 and 5 molecules were 3.0, 1.7, 1.8, 1.5 and 1.4 kbp, respectively. cDNA inserts to RNA 2 were covered at a part of the 3'regions, and those of RNAs 3,4 and 5 were almost full-length. The plasmids containing each of cDNA inserts were labeled with digoxigenin by the random priming method. Northern blot hybridization tests showed that individual probes hybridized specially to each of the five RNAs. Good results were obtained with 1 to 10 ng of RNA as a mixture of five RANs, but the probe to RNA 3, RNA 4 or RNA 5 gave a weak signal with hererologous RNAs when more than 10 ng RNA was used. In dot blot hybridization, the limit of detection was about 10 pg RNA, but if a higher content of RNA was spotted, cross reaction occurred using heterologous RNAs. For laboratory and field isolates of BNYVV, each of RNAs 3,4 and 5 was easily detected by Northern blot hybridization in total nucleic acids extracted from Tetragonia expansa leaves inoculated mechanically, but not from roots of sugar-beet plants inoculated by the fungus Polymyxa betae. However, satisfactory results were obtained with partially purified or concentrated preparations from roots. These findings indicate that the digoxigenin-labeled probes are useful for the identification and detection of RNAs contained in field and laboratory isolates of BNYVV. |
| キーワード | Sugar beet Rhizomania BNYVV RNA detection Nonradioactive cDNA probe |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 79 |
| 終了ページ | 96 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | The Characteristics of Trace Metal Distribution of Artificial and Natural-Origin Suspended Particulate Matter (SPM) by grain size |
|---|---|
| フルテキストURL | 006_001_029_041.pdf |
| 著者 | 村本 茂樹| 米谷 俊彦| 平岡 直子| 青山 勲| |
| 抄録 | Air pollution caused by suspended particulate matter (SPM) has increased remarkedly in industrial areas, and this has become an emergency issue in the global environment. Atomospheric SPM influences the local ecosystem including various plants and animals. It is necessary to examine the distribution characteristics of such trace metals in fine particulates (especially those with a diameter (φ) of less than 2.1μm). The Environment Agency of the Japanese goverment will complete analysis of the a concentration of suspended particulate matter less than 2.4μm by the end of 1999, and will be examining the relationship between the concentration of suspended particulate matter (0.1-9.0μm) with consideration given to the particle size throughout two years. It became clear that the metals of artificial origin such as Pb, V, Cd, Ni, Cr, existed in fine particulates (0.1 or more 2.0μm). By contrast, the metals of natural origin, such as Ti, Mn, Mg, Sr, mainly existed in coarse Area in Japan. |
| キーワード | Atomospheric Suspended particulate matter (SPM) Natural-origin metal Artificial-origin metal Biological effects |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1999 |
| 巻 | 6巻 |
| 号 | 1号 |
| 開始ページ | 29 |
| 終了ページ | 41 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 幼苗期と成熟期のオオムギ系統間における禾穀類アブラムシの密度とグラミン含量の関係 |
|---|---|
| フルテキストURL | 004_001_049_058.pdf |
| 著者 | Moharramipour Saeid| 村田 眞一| 兼久 勝夫| 積木 久明| |
| 抄録 | The relationship between the indole alkaloid gramine concentration and aphid population was examined seedling and maturation stages in 14 barley lines of Hordeum spontaneum and H. unlgare. The density of Schizaphis graminum did not differ significantly with the gramine concentration in the seedling in the greenhouse. However, the population of Rhopalosiphum padi sometimes differed with the seedling. The plant resistance to the natural infestation of cereal aphids was obvious at the heading stage. There was a negative correlation between the high population density of aphids and gramine concentration. The gramine concentration was high in matured resistant resistant lines, especially wild lines, as compared with susceptible lines due to higher biodegradation activity. |
| キーワード | Barley Resistance Gramine Cereal aphids |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 49 |
| 終了ページ | 58 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Relationship between Resistance to Cereal Aphids and Gramine Concentration in Beer Barley |
|---|---|
| フルテキストURL | 004_001_043_048.pdf |
| 著者 | 積木 久明| 笠原 敏彦| 兼久 勝夫| Saeid Moharramipour| |
| 抄録 | To beer barley resistance to cereal aphids, the relationship between the aphid density and gramine concentration was examined in various barley lines including beer barley cultivars. All beer barley cultivars tested were resistant or moderately resistant to the aphids, especially Asahi 5 and Haruna Nijo were similar to a wild line, H603 (W603), for checking resistant. However, these beer barleys contained much less gramine than the wild barley line, suggesting that another resistant factor(s) affects cereal aphid density. |
| キーワード | Beer barley Cereal aphid Resistance Gramine |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 43 |
| 終了ページ | 48 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | トウモロコシに含まれるDIMBOAとアブラムシ抵抗性の関係 |
|---|---|
| フルテキストURL | 004_001_033_042.pdf |
| 著者 | Rustamaki Maqsood A| 兼久 勝夫| 積木 久明| 白神 孝| |
| 抄録 | 2,4-Dihydrox-7-methoxy1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid is considered to be one of the components responsible for the resistance to pest insects in cereal plants. The relationship between concentration of DIMBOA and aphid infestation on 21 corn lines was investigated in 1990 and 1991. DIMBOA was detected in leaves od all corn lines tested, contained larger amounts in young plants and gradually decreased with growth. This property was the same as observed in wheat. A more than ten times difference in DIMBOA concentration was observed in corn lines. However, there was no positive correlation between DIMBOA concentration and aphid density. Most of the lines used had resistance to aphids. Resistance may be built together with other components such as (E)-aconitic acid. |
| キーワード | Apfid Resistant substance Hydroxamic acid DIMBOA Corn |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 33 |
| 終了ページ | 42 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Sorghum Resistance to the Sugarcane Aphid, Melanaphis sacchari (Zehntenr) Amounts of Surface Wax and Nutritional Components |
|---|---|
| フルテキストURL | 003_001_027_034.pdf |
| 著者 | 積木 久明| 兼久 勝夫| Moharramipour Saeid| |
| 抄録 | The differences in the amounts of leaf surface wax and nutritional components such as sugar and free amino acids were compared among resistant (PE 954177), moderately resistant (IS 84) and susceptible (Redlan B) sorghum varieties to the sugarcane aphid. Melanaphis sacchari (Zehntner). (1) The kind of aphid appearing on the leaves of the sorghum varieties was only the sugarcane aphid, Melanaphis sacchari in the field. The number of aphids infesting a leaf was ca 0,30 and 3,500 in the resistant, moderately resistant and susceptible varieties at the middle of August, respectively. (2) The amount of leaf surface wax was approximately constant among these three varieties. (3) The total sugar content was slightly higher in the two resistant varieties than in the susceptible variety. (4) The total free amino acid concentration was higher in the two resistant varieties than in the susceptible variety. (5) These findings suggest that neither leaf surface wax nor nutritional components seem to affect the aphid populations on the leaves of the three sorghum varieties, showing that biogenic substances such as dhurrin, benzylalcohol, p-hydroxybenzaldehyde, aconitic acide, etc. may affect the aphid populations. |
| キーワード | Sorghum Sugarcane aphid Melanaphis sacchari Leaf surface wax Amino acid |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 27 |
| 終了ページ | 34 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Sources of Resistance to Net Blotch in Barley Germplasm |
|---|---|
| フルテキストURL | 002_001_091_102.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | Net blotch caused by a fungus Pyrenophora teres Drechs. is a common disease in barley. Its source of resistance has been screened by many researchers by field evaluations or seedling tests inoculating a single isolatc. Since the pathogcnic variation of isolates has been reported in net blotch, resistance of the varieties to the disease may be different among the isolates with different pathogenicities. In this study, the pathogenic variation was examined and the varietal variation of the resistance was evaluated by inoculating with four P. teres isolates collected from Japan and Canada to more than 2,200 barley varieties of the world collection preserved at the Barley Germplasm Center of Okayama University. A preliminary inoculation test showed that the disease rating was affected little by the inoculation seasons. Disease ratings of varieties showed a continuous variation with a single mode in the resistant range in each of the four isolates. However, the correlation coefficient between Japanese isolate K105 and Canadian isolate WRS102 was as low as 0.55, indicating a slight pathogenic differentiation between these isolates. Significant correlation coefficients (r=.55~.78) among the ratings of isolates indicated that the pathogenicity to the varieties was rather similar and that the pathogenic differentiation was small among the four isolates tested. In general, varieties from Ethiopia, North Africa and Korea were more resistant than those from other regions. Varieties from Turkey and Europe were susceptible to Japanese isolates, while Nepalese varieties were susceptible to Canadian isolates. Twenty of 25 varieties which were resistant to the isolate K105 but susceptible to the isolate WR102 were from Nepal and most of those were Oriental-type (Bt bt2) in brittleness of rachis. These findings revealed an example of regional concentration of resistant gene in net blotch. |
| キーワード | Barley Net blotch Disease resistance Genetic resources Race differentiation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 91 |
| 終了ページ | 102 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313965 |
| タイトル（別表記） | 高対塩性オオムギ品種の選抜 |
|---|---|
| フルテキストURL | 001_002_113_129.pdf |
| 著者 | Sopandie Didy| 武田 和義| 森次 益三| 川﨑 利夫| |
| 抄録 | In order to select higher salt tolerant cultivars of barley,solution culture experiments were carried out with 500 barley cultivars, which were preliminarily selected from 4581 cultivars by exposure to 1-2% of sodium chloride(MaCl) during the germinating stage. The concentrations of NaCl used in the experiments were 2 (control), 150 and 300 mM in nutrient solution.From the results of five solution culture experiments, each of which was carried out with about 100 cultivars, 105 cultivars of barley were selected as relatively about 100 cultivars. Then the final selection was carried out on those 105 cultivars, and it was found that more than 10 cultivars were highly salt tolerant, mainly based on the comparisons of shoot yields under the supply of 300 mM of NaCl.High salt tolerant barley cultivars mentioned above showed 42-86% and 17-30% of the control in shoot yield under the condition of 150 and 300 mM of NaCl, respectively.On three groups of barley,each having more than 10 cultivars, which are high, moderate and low in salt tolerance, ion contents of the plants were analyzed.The results suggest that potassium(K) uptake was comparatively larger in the high salt tolerant goup of barley cultivars,through there was a big variation in sodium(Na) and K contents even within the same group. |
| キーワード | Barley Ion content Salt tolerance Sodium chloride Yield |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 113 |
| 終了ページ | 129 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 硫化カドミウムの高濃度添加時における小麦の生育とカドミウム集積濃度に対する炭酸カルシウムの影響 |
|---|---|
| フルテキストURL | 001_001_001_008.pdf |
| 著者 | 村本 茂樹| 西崎 日佐夫| 青山 勲| |
| 抄録 | In Japan, wheat is usually cultivated from December to June as a second crop in the same field as rice. In Cd-polluted areas, the fields are affected by both wastewater and the emissions from the chimneys of zinc refineries or plating factories. The application of inhibition agents, such as slaked lime, calcium silicate or fused magnesium are applied to inhibit the absorption of these metals by plants. Many reports have been made on the decrease of metal absorption through the application of some form of calcium, phosphate or silicate. Also, it has been reported that wheat is more sensitive to CdS than to CdO. However, almost all experiments were performed at less than 500 ppm Cd in soil, and these experiments have not been made at a concentration close to the level critical for wheat plants. There have been few reports on the effects of calcium cabonate on metal tolerance or metal uptake at the a level critical wheat. The typical example of using calcium carbonate was investigated by Maeda, and the application of phosphate by Koshino, and by Muramoto et al. The effects of calcium carbonate on the metal content of wheat to the critical levels were examined. |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 1 |
| 終了ページ | 8 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| 著者 | 稲葉 英男| 今井 誠士| |
|---|---|
| 発行日 | 1995-05-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 61巻 |
| 号 | 585号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 今井 誠士| |
|---|---|
| 発行日 | 1995-06-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 61巻 |
| 号 | 586号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 森田 慎一| 野津 滋| |
|---|---|
| 発行日 | 1993-09-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 59巻 |
| 号 | 565号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 武谷 健吾| 野津 滋| |
|---|---|
| 発行日 | 1993-04-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 59巻 |
| 号 | 560号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 宮原 里支| 武谷 健吾| |
|---|---|
| 発行日 | 1995-09-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 61巻 |
| 号 | 589号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 森田 慎一| |
|---|---|
| 発行日 | 1995-05-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 61巻 |
| 号 | 585号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 堀部 明彦| 高本 直樹| 川上 理亮| 今井 誠士| |
|---|---|
| 発行日 | 2000-10-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 66巻 |
| 号 | 650号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 今井 誠士| |
|---|---|
| 発行日 | 1997-03-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 63巻 |
| 号 | 607号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 金 明俊| 堀部 明彦| |
|---|---|
| 発行日 | 2002-01-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 68巻 |
| 号 | 665号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 堀部 明彦| 春木 直人| 村上 昌弘| |
|---|---|
| 発行日 | 2002-05-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 68巻 |
| 号 | 669号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 稲葉 英男| 金 明俊| 堀部 明彦| |
|---|---|
| 発行日 | 2002-09-25 |
| 出版物タイトル | 日本機械学會論文集 B編 |
| 巻 | 68巻 |
| 号 | 673号 |
| 資料タイプ | 学術雑誌論文 |