ID | 16309 |
Eprint ID | 16309
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フルテキストURL | |
タイトル(別表記) | Studies on galactosyl transferase activity of gastric mucous in rats Part 1 Studies on an assay method and changes in gastric mucosal UDP-galactosyl transferase activity during fasting
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著者 |
永原 章正
岡山大学医学部第一内科教室
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抄録 | A method to measure the activity of an enzyme catalyzing sugar chains which are abundant in O-glycosidic linkage was studied using UDP-galactosyl transferase (UDP-Gal-T) found in the gastric mucosa of rat and asialo-bovine-submaxillar-mucin (ASBSM) as a substrate. The optimum pH for the measurement was 7.5, Mn(++) was required, and about twice as much Triton X-100 was needed as when asialo-agalacto-fetuin (ASGF) was used as the substrate. The activity of UDP-Gal-T was measured simultaneously in a group of rats receiving food and a group fasted for 24 hours, using ASGF and ASBSM as substrates. With ASGF no significant difference was seen in either the glandular corpus or the pylorus, while with ASBSM, the activity decreased significantly (p<0.05) in the glandular corpus, though no significant difference was seen in the pylorus.
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キーワード | UDP-galactosyl transferase
asialo-agalacto-fetuin
asialo-bovine-submaxillar-mucin
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発行日 | 1986-06-30
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出版物タイトル |
岡山医学会雑誌
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出版物タイトル(別表記) | Journal of Okayama Medical Association
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巻 | 98巻
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号 | 7-8号
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出版者 | 岡山医学会
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出版者(別表記) | Okayama Medical Association
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開始ページ | 627
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終了ページ | 633
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ISSN | 0030-1558
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NCID | AN00032489
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資料タイプ |
学術雑誌論文
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オフィシャル URL | https://www.jstage.jst.go.jp/article/joma1947/98/7-8/98_7-8_627/_article/-char/ja/
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関連URL | http://www.okayama-u.ac.jp/user/oma/index.html
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言語 |
日本語
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著作権者 | 岡山医学会
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論文のバージョン | publisher
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査読 |
有り
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Eprints Journal Name | joma
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