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ID 16309
Eprint ID
16309
フルテキストURL
98_627.pdf 295 KB
タイトル(別表記)
Studies on galactosyl transferase activity of gastric mucous in rats Part 1 Studies on an assay method and changes in gastric mucosal UDP-galactosyl transferase activity during fasting
著者
永原 章正 岡山大学医学部第一内科教室
抄録
A method to measure the activity of an enzyme catalyzing sugar chains which are abundant in O-glycosidic linkage was studied using UDP-galactosyl transferase (UDP-Gal-T) found in the gastric mucosa of rat and asialo-bovine-submaxillar-mucin (ASBSM) as a substrate. The optimum pH for the measurement was 7.5, Mn(++) was required, and about twice as much Triton X-100 was needed as when asialo-agalacto-fetuin (ASGF) was used as the substrate. The activity of UDP-Gal-T was measured simultaneously in a group of rats receiving food and a group fasted for 24 hours, using ASGF and ASBSM as substrates. With ASGF no significant difference was seen in either the glandular corpus or the pylorus, while with ASBSM, the activity decreased significantly (p<0.05) in the glandular corpus, though no significant difference was seen in the pylorus.
キーワード
UDP-galactosyl transferase
asialo-agalacto-fetuin
asialo-bovine-submaxillar-mucin
発行日
1986-06-30
出版物タイトル
岡山医学会雑誌
出版物タイトル(別表記)
Journal of Okayama Medical Association
98巻
7-8号
出版者
岡山医学会
出版者(別表記)
Okayama Medical Association
開始ページ
627
終了ページ
633
ISSN
0030-1558
NCID
AN00032489
資料タイプ
学術雑誌論文
オフィシャル URL
https://www.jstage.jst.go.jp/article/joma1947/98/7-8/98_7-8_627/_article/-char/ja/
関連URL
http://www.okayama-u.ac.jp/user/oma/index.html
言語
日本語
著作権者
岡山医学会
論文のバージョン
publisher
査読
有り
Eprints Journal Name
joma