start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=3 article-no= start-page=104810 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=202503 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts en-subtitle= kn-subtitle= en-abstract= kn-abstract=Molecular biological techniques have enabled the accurate identification of the avian Eimeria parasite, however, the preparation of PCR template remains a bottleneck due to contaminants from feces and the robust oocyst's wall resistant to chemical and mechanical force. Generally, the preparation of PCR template involves three main steps: (1) pretreatment of oocysts; (2) disruption of oocysts; and (3) purification of genomic DNA. We prepared PCR templates from both unsporulated and sporulated E. tenella oocysts using various protocols, followed by species-specific PCR to define the limit of detection. Our data revealed that whereas neither pretreatment of oocysts with sodium hypochlorite nor purification of genomic DNA with commercial kits improved the limit of detection of PCR, disruption of oocysts was a critical step in the preparation of PCR templates. The most sensitive PCR assay was achieved with the template prepared by disrupting oocysts suspended in distilled water, followed by bead-beating and heating at 99°C for 5 min, which detected 0.16 oocysts per PCR. This ultra-simplified protocol for preparation of PCR template, which does not require expensive reagents or equipment, will significantly enhance the sensitive and efficient molecular identification of Eimeria. It will improve our understanding of the prevalence of this parasite at the species level and contribute to the development of techniques for the control in the field. en-copyright= kn-copyright= en-aut-name=TakanoAruto en-aut-sei=Takano en-aut-mei=Aruto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=UmaliDennis V. en-aut-sei=Umali en-aut-mei=Dennis V. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WardhanaApril H. en-aut-sei=Wardhana en-aut-mei=April H. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SawitriDyah H. en-aut-sei=Sawitri en-aut-mei=Dyah H. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TeramotoIsao en-aut-sei=Teramoto en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=HatabuToshimitsu en-aut-sei=Hatabu en-aut-mei=Toshimitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KidoYasutoshi en-aut-sei=Kido en-aut-mei=Yasutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KanekoAkira en-aut-sei=Kaneko en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SasaiKazumi en-aut-sei=Sasai en-aut-mei=Kazumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KatohHiromitsu en-aut-sei=Katoh en-aut-mei=Hiromitsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=MatsubayashiMakoto en-aut-sei=Matsubayashi en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= affil-num=1 en-affil=Departments of Veterinary Immunology, Graduate School of Veterinary Medical Sciences, Osaka Metropolitan University kn-affil= affil-num=2 en-affil=Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of the Philippines Los Ba?os, College kn-affil= affil-num=3 en-affil=Research Center for Veterinary Science, National Research and Innovation Agency kn-affil= affil-num=4 en-affil=Research Center for Veterinary Science, National Research and Innovation Agency kn-affil= affil-num=5 en-affil=Departments of Virology and Parasitology, Graduate School of Medicine, Osaka Metropolitan University kn-affil= affil-num=6 en-affil=Laboratory of Animal Physiology, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=7 en-affil=Departments of Virology and Parasitology, Graduate School of Medicine, Osaka Metropolitan University kn-affil= affil-num=8 en-affil=Departments of Virology and Parasitology, Graduate School of Medicine, Osaka Metropolitan University kn-affil= affil-num=9 en-affil=Departments of Veterinary Immunology, Graduate School of Veterinary Medical Sciences, Osaka Metropolitan University kn-affil= affil-num=10 en-affil=Departments of Veterinary Immunology, Graduate School of Veterinary Medical Sciences, Osaka Metropolitan University kn-affil= affil-num=11 en-affil=Departments of Veterinary Immunology, Graduate School of Veterinary Medical Sciences, Osaka Metropolitan University kn-affil= en-keyword=Coccidian parasite kn-keyword=Coccidian parasite en-keyword=Eimeria tenella kn-keyword=Eimeria tenella en-keyword=Extraction kn-keyword=Extraction en-keyword=Molecular identification kn-keyword=Molecular identification en-keyword=Oocyst kn-keyword=Oocyst END start-ver=1.4 cd-journal=joma no-vol=238 cd-vols= no-issue= article-no= start-page=120296 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2025 dt-pub=20250505 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Grafting-through functionalization of graphene oxide with cationic polymers for enhanced adsorption of anionic dyes and viruses en-subtitle= kn-subtitle= en-abstract= kn-abstract=Graphene oxide (GO) is a sheet-like carbon material with abundant oxygen-containing functional groups on its surface. GO has been extensively studied as an adsorbent for heavy metals and organic compounds. However, effective strategies for negatively charged materials have yet to be established. This study aimed to synthesize composites of GO and cationic polymers for the selective adsorption of negatively charged materials; a challenge in this approach is the strong electrostatic interactions between GO and cationic polymers, which can lead to aggregation. This study addresses this issue by employing the grafting-through method. GO was initially modified with allylamine to introduce a polymerizable site, followed by radical polymerization to covalently bond polymers to the GO surface, effectively preventing aggregation. Adsorption experiments demonstrated that the GO-polymer composite selectively adsorbs anionic dye, such as methyl orange. Virus adsorption tests showed significantly enhanced performance compared to pristine GO. These results emphasize the critical role of controlled surface modification and charge manipulation in optimizing the adsorption performance of GO. This study establishes a simple and effective approach for synthesizing GO-cationic polymer composites, contributing to the development of advanced materials for water purification applications. en-copyright= kn-copyright= en-aut-name=KimuraRyota en-aut-sei=Kimura en-aut-mei=Ryota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Ferr?-PujolPilar en-aut-sei=Ferr?-Pujol en-aut-mei=Pilar kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NishinaYuta en-aut-sei=Nishina en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= affil-num=2 en-affil=Research Core for Interdisciplinary Sciences, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University kn-affil= en-keyword=Graphene oxide kn-keyword=Graphene oxide en-keyword=Virus adsorption kn-keyword=Virus adsorption en-keyword=Dye adsorption kn-keyword=Dye adsorption en-keyword=Cationic polymer composites kn-keyword=Cationic polymer composites en-keyword=Adsorbent kn-keyword=Adsorbent en-keyword=Aggregation kn-keyword=Aggregation END start-ver=1.4 cd-journal=joma no-vol=18 cd-vols= no-issue=49 article-no= start-page=33264 end-page=33275 dt-received= dt-revised= dt-accepted= dt-pub-year=2024 dt-pub=20241122 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Mass Production of Graphene Oxide Beyond the Laboratory: Bridging the Gap Between Academic Research and Industry en-subtitle= kn-subtitle= en-abstract= kn-abstract=The mass production of graphene oxide (GO) has garnered significant attention in recent years due to its potential applications in various fields, from materials science to biomedicine. Graphene, known for its unique properties, such as high conductivity and mechanical strength, has been extensively studied. However, traditional production methods such as the exfoliation of graphite with scotch tape are not suitable for large-scale production. This has led to an increased focus on GO as a viable alternative to graphene production. Nonetheless, challenges, including the optimization of oxidation processes, the control of structural uniformity, and the reproducibility of production, have not been solved so far. This review critically examines GO production advancements by analyzing experimental and mechanistic studies to identify significant developments that enable high-yield and reproducible methods suitable for industrial-scale production. Special attention is given to oxidation techniques and postsynthesis purification and storage, with a focus on controlled oxidation to achieve homogeneous and single-layer GO. Through this lens, the review outlines the path forward for the industrialization of GO, aiming to bridge the gap between academic research and industrial production. en-copyright= kn-copyright= en-aut-name=NishinaYuta en-aut-sei=Nishina en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Research Institute for Interdisciplinary Science, Okayama University kn-affil= en-keyword=Graphene oxide kn-keyword=Graphene oxide en-keyword=Graphite kn-keyword=Graphite en-keyword=Chemical oxidation kn-keyword=Chemical oxidation en-keyword=Electrochemical oxidation kn-keyword=Electrochemical oxidation en-keyword=Mass production kn-keyword=Mass production en-keyword=Purification kn-keyword=Purification en-keyword=Optimization kn-keyword=Optimization en-keyword=Industrialization kn-keyword=Industrialization en-keyword=Safety kn-keyword=Safety en-keyword=Stability kn-keyword=Stability END start-ver=1.4 cd-journal=joma no-vol=149 cd-vols= no-issue= article-no= start-page=13 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=2018 dt-pub=201809 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Functional analysis of N-terminal propeptide in the precursor of Vibrio vulnificus metalloprotease by using cell-free translational system en-subtitle= kn-subtitle= en-abstract= kn-abstract=Vibrio vulnificus is a human pathogen causing fatal septicemia with edematous and hemorrhagic skin damage. Among multiple virulence factors, an extracellular metalloprotease termed as V. vulnificus protease (VVP) is known to play a crucial role in eliciting the skin damage. The mature VVP (413 aa) is composed of two domains, the N-terminal core domain with proteolytic activity and the C-terminal domain mediates efficient attachment to protein substrates. However, VVP is produced as an inactive precursor (609 aa) with a signal peptide (24 aa) and propeptide (172 aa). In order to clarify the function of propeptide, a series of DNA fragments encoding the VVP precursor and its various domains were designed and the proteins were expressed in vitro by using cell-free translational system. The results indicated that the propeptide might function as an intramolecular chaperon to promote the proper folding of both N-terminal and C-terminal domains. The obtained results also suggest that the propeptide, itself was unstable and thus digested easily by the enzymes present in cell lysate used for cell-free system. Additionally, the C-terminal domain in VVP found to inhibit the folding of the N-terminal domain in absence of propeptide. en-copyright= kn-copyright= en-aut-name=KawaseTomoka en-aut-sei=Kawase en-aut-mei=Tomoka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiuraFumi en-aut-sei=Miura en-aut-mei=Fumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=DebnathAnusuya en-aut-sei=Debnath en-aut-mei=Anusuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ImakuraKinuyo en-aut-sei=Imakura en-aut-mei=Kinuyo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MiyoshiShin-ichi en-aut-sei=Miyoshi en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=Vibrio vulnificus kn-keyword=Vibrio vulnificus en-keyword=Protease kn-keyword=Protease en-keyword=Propeptide kn-keyword=Propeptide en-keyword=Domain kn-keyword=Domain en-keyword=Cell-free translational system kn-keyword=Cell-free translational system END start-ver=1.4 cd-journal=joma no-vol=3 cd-vols= no-issue=1 article-no= start-page=101046 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220318 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Expression of microbial rhodopsins in Escherichia coli and their extraction and purification using styrene-maleic acid copolymers en-subtitle= kn-subtitle= en-abstract= kn-abstract=Microbial rhodopsins are photoreceptive membrane proteins showing various light-dependent biological activities. Styrene-maleic acid (SMA) copolymers spontaneously form nanoscale lipid particles containing membrane proteins and associated lipids without detergent, and can be used to characterize membrane molecules. Here, we provide a protocol to functionally express a thermally stable rhodopsin, Rubrobacter xylanophilus rhodopsin, and an unstable rhodopsin, Halobacterium salinarum sensory rhodopsin I, in Escherichia coli. We then describe the preparation of SMA and the extraction and purification of rhodopsin molecules using SMA.
For complete details on the use and execution of this protocol, please refer to Ueta et al. (2020). en-copyright= kn-copyright= en-aut-name=KojimaKeiichi en-aut-sei=Kojima en-aut-mei=Keiichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SudoYuki en-aut-sei=Sudo en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= affil-num=1 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=263 end-page=272 dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=202306 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Early Fluid Balance Is Associated with 90-Day Mortality in Patients Receiving Continuous Renal Replacement Therapy en-subtitle= kn-subtitle= en-abstract= kn-abstract=Continuous renal replacement therapy (CRRT) is widely used to control fluid balance, but the optimal fluid balance to improve the prognosis of patients remains debated. Appropriate fluid management may depend on hemodynamic status. We investigated the association between 90-day mortality and fluid balance/mean arterial pressure (MAP) in patients receiving CRRT. This single-center retrospective study was conducted between May 2018 and March 2021. Based on the cumulative fluid balance at 72 h after initiation of CRRT, the cases were divided into negative (< 0 mL) and positive (> 0 mL) fluid balance groups. Ninety-day mortality was higher in the positive fluid balance group (p=0.009). At 4 h before and after CRRT initiation, the mean MAP was lower in the positive fluid balance group (p<0.05). After multivariate cox adjustment, 72-h positive fluid balance was independently associated with 90-day mortality (p=0.004). In addition, the cumulative fluid balance was associated with 90-day mortality (p<0.05) in cases without shock, high APACHE II score, sepsis, dialysis dependence, or vasopressor use. A 72-h positive fluid balance was associated with 90-day mortality in patients receiving CRRT. en-copyright= kn-copyright= en-aut-name=GuoYusheng en-aut-sei=Guo en-aut-mei=Yusheng kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KosakaJunko en-aut-sei=Kosaka en-aut-mei=Junko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MorimatsuHiroshi en-aut-sei=Morimatsu en-aut-mei=Hiroshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil=Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Anesthesiology and Resuscitology, Okayama University Hospital kn-affil= affil-num=3 en-affil=Department of Anesthesiology and Resuscitology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=fluid management kn-keyword=fluid management en-keyword=continuous renal replacement therapy kn-keyword=continuous renal replacement therapy en-keyword=mortality kn-keyword=mortality en-keyword=mean arterial pressure kn-keyword=mean arterial pressure en-keyword=daily cumulative fluid balance kn-keyword=daily cumulative fluid balance END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2023 dt-pub=20230324 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=西南日本中国地方の後期新生代火山岩のハフニウム・ホウ素・リチウム・マグネシウム同位体システマティクス：沈み込み帯で発生するスラブ由来の流体の特性と輸送メカニズムの解明 kn-title=Hf-B-Li-Mg isotope systematics of late Cenozoic volcanic rocks from the Chugoku district, Southwest Japan: Implications for the property and transport mechanism of slab-derived fluids in the subduction zone en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ZHANGWei en-aut-sei=ZHANG en-aut-mei=Wei kn-aut-name=?? kn-aut-sei=? kn-aut-mei=? aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Natural Science and Technology, Okayama university kn-affil=岡山大学大学院自然科学研究科 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=2 article-no= start-page=146 end-page=154 dt-received= dt-revised= dt-accepted= dt-pub-year=2022 dt-pub=20220201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Development of Scaled-Up Synthetic Method for Retinoid X Receptor Agonist NEt-3IB Contributing to Sustainable Development Goals en-subtitle= kn-subtitle= en-abstract= kn-abstract=Abstract Small-molecular drugs, which are generally inexpensive compared with biopharmaceuticals and can often be taken orally, may contribute to the Sustainable Development Goals (SDGs) adopted by the United Nations. We previously reported the retinoid X receptor (RXR) agonist 4-(ethyl(3-isobutoxy-4-isopropylphenyl)amino)benzoic acid (NEt-3IB, 1) as a small-molecular drug candidate to replace biopharmaceuticals for the treatment of inflammatory bowel disease. The previous synthetic method to 1 required a large amount of organic solvent and extensive purification. In line with the SDGs, we aimed to develop an environmentally friendly, inexpensive method for the large-scale synthesis of 1. The developed method requires only a hydrophobic ether and EtOH as reaction and extraction solvents. The product was purified by recrystallization twice to afford 99% pure 1 at 100?mmol scale in about 30% yield. The optimized process showed a 35-fold improvement of the E-factor (an index of environmental impact) compared to the original method. This work, which changes the solvent used to environmentally preferable ones based on the existing synthetic method for 1, illustrates how synthetic methods for small-molecular drugs can be adapted and improved to contribute to the SDGs. en-copyright= kn-copyright= en-aut-name=TakamuraYuta en-aut-sei=Takamura en-aut-mei=Yuta kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MorishitaKen-ichi en-aut-sei=Morishita en-aut-mei=Ken-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KikuzawaShota en-aut-sei=Kikuzawa en-aut-mei=Shota kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeMasaki en-aut-sei=Watanabe en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=KakutaHiroki en-aut-sei=Kakuta en-aut-mei=Hiroki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=4 en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=5 en-affil=Division of Pharmaceutical Sciences, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= END start-ver=1.4 cd-journal=joma no-vol=12 cd-vols= no-issue= article-no= start-page=647684 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210810 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification, Characterization, and Gene Expression of Rice Endo-beta-N-Acetylglucosaminidase, Endo-Os en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the endoplasmic reticulum-associated degradation system of plant and animal cells, high-mannose type free N-glycans (HMT-FNGs) are produced from misfolded glycoproteins prior to proteasomal degradation, and two enzymes, cytosolic peptide:N-glycanase (cPNGase) and endo-beta-N-acetylglucosaminidase (endo-beta-GlcNAc-ase), are involved in the deglycosylation. Although the physiological functions of these FNGs in plant growth and development remain to be elucidated, detailed characterization of cPNGase and endo-beta-GlcNAc-ase is required. In our previous work, we described the purification, characterization, and subcellular distribution of some plant endo-beta-GlcNAc-ases and preliminarily reported the gene information of rice endo-beta-GlcNAc-ase (Endo-Os). Furthermore, we analyzed the changes in gene expression of endo-beta-GlcNAc-ase during tomato fruit maturation and constructed a mutant line of Arabidopsis thaliana, in which the two endo-beta-GlcNAc-ase genes were knocked-out based on the Endo-Os gene. In this report, we describe the purification, characterization, amino acid sequence, and gene cloning of Endo-Os in detail. Purified Endo-Os, with an optimal pH of 6.5, showed high activity for high-mannose type N-glycans bearing the Man alpha 1-2Man alpha 1-3Man beta 1 unit; this substrate specificity was almost the same as that of other plant endo-beta-GlcNAc-ases, suggesting that Endo-Os plays a critical role in the production of HTM-FNGs in the cytosol. Electrospray ionization-mass spectrometry analysis of the tryptic peptides revealed 17 internal amino acid sequences, including the C terminus; the N-terminal sequence could not be identified due to chemical modification. These internal amino acid sequences were consistent with the amino acid sequence (UniProt ID: Q5W6R1) deduced from the Oryza sativa cDNA clone AK112067 (gene ID: Os05g0346500). Recombinant Endo-Os expressed in Escherichia coli using cDNA showed the same enzymatic properties as those of native Endo-Os. en-copyright= kn-copyright= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OkamotoNaoko en-aut-sei=Okamoto en-aut-mei=Naoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ArakiNode en-aut-sei=Araki en-aut-mei=Node kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=2 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=3 en-affil=Department of Tumor Genetics and Biology, Graduate School of Medical Sciences, Kumamoto University kn-affil= affil-num=4 en-affil=Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University kn-affil= en-keyword=endo-beta-N-acetylglucosaminidase kn-keyword=endo-beta-N-acetylglucosaminidase en-keyword=free N-glycans kn-keyword=free N-glycans en-keyword=Oryza sativa kn-keyword=Oryza sativa en-keyword=ER associated degradation kn-keyword=ER associated degradation en-keyword=peptide:N-glycanase kn-keyword=peptide:N-glycanase END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=6 article-no= start-page=1328 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2021 dt-pub=20210527 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Exosome-Based Molecular Transfer Activity of Macrophage-Like Cells Involves Viability of Oral Carcinoma Cells: Size Exclusion Chromatography and Concentration Filter Method en-subtitle= kn-subtitle= en-abstract= kn-abstract=Extracellular vesicles (EV) heterogeneity is a crucial issue in biology and medicine. In addition, tumor-associated macrophages are key components in cancer microenvironment and immunology. We developed a combination method of size exclusion chromatography and concentration filters (SEC-CF) and aimed to characterize different EV types by their size, cargo types, and functions. A human monocytic leukemia cell line THP-1 was differentiated to CD14-positive macrophage-like cells by stimulation with PMA (phorbol 12-myristate 13-acetate) but not M1 or M2 types. Using the SEC-CF method, the following five EV types were fractionated from the culture supernatant of macrophage-like cells: (i) rare large EVs (500-3000 nm) reminiscent of apoptosomes, (ii) EVs (100-500 nm) reminiscent of microvesicles (or microparticles), (iii) EVs (80-300 nm) containing CD9-positive large exosomes (EXO-L), (iv) EVs (20-200 nm) containing unidentified vesicles/particles, and (v) EVs (10-70 nm) containing CD63/HSP90-positive small exosomes (EXO-S) and particles. For a molecular transfer assay, we developed a THP-1-based stable cell line producing a GFP-fused palmitoylation signal (palmGFP) associated with the membrane. The THP1/palmGFP cells were differentiated into macrophages producing palmGFP-contained EVs. The macrophage/palmGFP-secreted EXO-S and EXO-L efficiently transferred the palmGFP to receiver human oral carcinoma cells (HSC-3/palmTomato), as compared to other EV types. In addition, the macrophage-secreted EXO-S and EXO-L significantly reduced the cell viability (ATP content) in oral carcinoma cells. Taken together, the SEC-CF method is useful for the purification of large and small exosomes with higher molecular transfer activities, enabling efficient molecular delivery to target cells. en-copyright= kn-copyright= en-aut-name=LuYanyin en-aut-sei=Lu en-aut-mei=Yanyin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=EguchiTakanori en-aut-sei=Eguchi en-aut-mei=Takanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SogawaChiharu en-aut-sei=Sogawa en-aut-mei=Chiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TahaEman A. en-aut-sei=Taha en-aut-mei=Eman A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TranManh Tien en-aut-sei=Tran en-aut-mei=Manh Tien kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NaraToshiki en-aut-sei=Nara en-aut-mei=Toshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=WeiPenggong en-aut-sei=Wei en-aut-mei=Penggong kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=FukuokaShiro en-aut-sei=Fukuoka en-aut-mei=Shiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MiyawakiTakuya en-aut-sei=Miyawaki en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OkamotoKuniaki en-aut-sei=Okamoto en-aut-mei=Kuniaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Research Program for Undergraduate Students, Okayama University Dental School kn-affil= affil-num=7 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Dental Anesthesiology and Special Care Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=10 en-affil=Department of Dental Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=macrophage kn-keyword=macrophage en-keyword=exosomes kn-keyword=exosomes en-keyword=extracellular vesicles kn-keyword=extracellular vesicles en-keyword=molecular transfer kn-keyword=molecular transfer en-keyword=size exclusion chromatography and concentration filter (SEC-CF) method kn-keyword=size exclusion chromatography and concentration filter (SEC-CF) method en-keyword=heat shock proteins kn-keyword=heat shock proteins en-keyword=oral carcinoma kn-keyword=oral carcinoma END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200925 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=主要ピーナツアレルゲンAra h1の新規アイソフォームの精製と分子キャラクタリゼーション kn-title=Purification and molecular characterization of a new isoform of Ara h1, a major peanut allergen en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=Asaduzzaman MD en-aut-sei=Asaduzzaman MD en-aut-mei= kn-aut-name=ASADUZZAMAN MD kn-aut-sei=ASADUZZAMAN MD kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 END start-ver=1.4 cd-journal=joma no-vol=325 cd-vols= no-issue= article-no= start-page=108645 end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200716 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Isolation and identification of the antimicrobial substance included in tempeh using Rhizopus stolonifer NBRC 30816 for fermentation en-subtitle= kn-subtitle= en-abstract= kn-abstract=In this study, we focus on the antimicrobial properties of tempeh, a soybean fermented food, against oral bacteria.
Tempeh showed antimicrobial activity against dental caries pathogenic bacterium Streptococcus mutans at a final concentration of 1 mg/mL. An antimicrobial substance contained in tempeh was present in the 100 kDa or greater fraction generated by ultrafiltration, but it was found not to be proteinaceous by native-PAGE, SDS-PAGE and protein degradation tests. Next, when the fraction was purified with an ODS column, the 80% and 100% methanol eluates showed antimicrobial activity against S. mutans. The 100% methanol eluate was further subjected to a 2nd column purification, and isolation of the target was confirmed by HPLC. When the isolated material was analyzed by ESI-MS, the m/z was 279.234. Further analysis by Raman spectroscopy revealed a peak similar to linoleic acid. This substance also possessed antimicrobial properties equivalent to linoleic acid. en-copyright= kn-copyright= en-aut-name=ItoMasahiro en-aut-sei=Ito en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=ItoTakashi en-aut-sei=Ito en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=AokiHideyuki en-aut-sei=Aoki en-aut-mei=Hideyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=NishiokaKoshi en-aut-sei=Nishioka en-aut-mei=Koshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ShiokawaTsugumi en-aut-sei=Shiokawa en-aut-mei=Tsugumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TadaHiroko en-aut-sei=Tada en-aut-mei=Hiroko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=TakeuchiYuki en-aut-sei=Takeuchi en-aut-mei=Yuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=TakeyasuNobuyuki en-aut-sei=Takeyasu en-aut-mei=Nobuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamamotoTadashi en-aut-sei=Yamamoto en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TakashibaShogo en-aut-sei=Takashiba en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil=Department of Pathophysiology - Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=2 en-affil=Department of Pathophysiology - Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=3 en-affil=Ikeda Food Research Co., Ltd. kn-affil= affil-num=4 en-affil=Ikeda Food Research Co., Ltd. kn-affil= affil-num=5 en-affil=Division of Instrumental Analysis, Department of Instrumental Analysis and Cryogenics, Advanced Science Research Center, Okayama University kn-affil= affil-num=6 en-affil=Division of Instrumental Analysis, Department of Instrumental Analysis and Cryogenics, Advanced Science Research Center, Okayama University kn-affil= affil-num=7 en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=8 en-affil=Department of Chemistry, Graduate School of Natural Science and Technology, Okayama University kn-affil= affil-num=9 en-affil=Department of Pathophysiology - Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=10 en-affil=Department of Pathophysiology - Periodontal Science, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= en-keyword=Fermented soybean food kn-keyword=Fermented soybean food en-keyword=Oral infection kn-keyword=Oral infection en-keyword=Antibacterial kn-keyword=Antibacterial en-keyword=Linoleic acid kn-keyword=Linoleic acid END start-ver=1.4 cd-journal=joma no-vol=109 cd-vols= no-issue= article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2020 dt-pub=20200201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Recombinant expression and characterization of quinone-containing novel glycine oxidase from Marinomonas mediterranea kn-title=Marinomonas mediterranea由来キノン含有新規グリシンオキシダーゼの大腸菌発現系の確立と性質検討 en-subtitle= kn-subtitle= en-abstract=, , , kn-abstract= 　Novel glycine oxidase (GlyOX) from Marinomonas mediterranea depends on cysteine tryptophilquinone (CTQ) and catalyzes the oxidative deamination of glycine to produce a glyoxylate, ammonia, and hydrogen peroxide. M. mediterranea GlyOX genes (goxA and goxB) were cloned and recombinant GlyOX was heterologously expressed by E. coli. The purification of recombinant GlyOX was carried out by metal affinity and DEAE-Toyopearl 650M column chromatographies. M. mediterranea GlyOX was homotetramic with a molecular mass of 76kDa and showed optimum activity around 30°C and at pH 5.0, and stability below 50°C and between pH 5.0 to 9.0. M. mediterranea GlyOX shows a strict substrate specificity toward glycine, and the Michaelis constant for glycine was 0.5mM. M. mediterranea GlyOX could determine the quantity of glycine in human serum and human blood plasma with high sensitivity. This study revealed the catalytic and structural properties of M. mediterranea GlyOX with high substrate specificity. en-copyright= kn-copyright= en-aut-name=KajiyamaYuki en-aut-sei=Kajiyama en-aut-mei=Yuki kn-aut-name=梶山雄輝 kn-aut-sei=梶山 kn-aut-mei=雄輝 aut-affil-num=1 ORCID= en-aut-name=MizobataSatsuki en-aut-sei=Mizobata en-aut-mei=Satsuki kn-aut-name=溝端佐津紀 kn-aut-sei=溝端 kn-aut-mei=佐津紀 aut-affil-num=2 ORCID= en-aut-name=AkajiShusaku en-aut-sei=Akaji en-aut-mei=Shusaku kn-aut-name=赤地周作 kn-aut-sei=赤地 kn-aut-mei=周作 aut-affil-num=3 ORCID= en-aut-name=NemotoMichiko en-aut-sei=Nemoto en-aut-mei=Michiko kn-aut-name=根本理子 kn-aut-sei=根本 kn-aut-mei=理子 aut-affil-num=4 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=5 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=6 ORCID= affil-num=1 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=2 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=3 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=4 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=5 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=6 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 en-keyword=glycine oxidase kn-keyword=glycine oxidase en-keyword=Marinomonas mediterranea kn-keyword=Marinomonas mediterranea en-keyword=cysteine tryptophilquinone kn-keyword=cysteine tryptophilquinone en-keyword=recombinant expression kn-keyword=recombinant expression en-keyword=enzymatic glycine assay kn-keyword=enzymatic glycine assay END start-ver=1.4 cd-journal=joma no-vol=91 cd-vols= no-issue= article-no= start-page=22 end-page=31 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20191115 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Collagen adhesion gene is associated with blood stream infections caused by methicillin-resistant Staphylococcus aureus en-subtitle= kn-subtitle= en-abstract= kn-abstract=Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) causes hospital- and community-acquired infections. It is not clear whether genetic characteristics of the bacteria contribute to disease pathogenesis in MRSA infection. We hypothesized that whole genome analysis of MRSA strains could reveal the key gene loci and/or the gene mutations that affect clinical manifestations of MRSA infection.
Methods: Whole genome sequences (WGS) of MRSA of 154 strains were analyzed with respect to clinical manifestations and data. Further, we evaluated the association between clinical manifestations in MRSA infection and genomic information.
Results: WGS revealed gene mutations that correlated with clinical manifestations of MRSA infection. Moreover, 12 mutations were selected as important mutations by Random Forest analysis. Cluster analysis revealed strains associated with a high frequency of bloodstream infection (BSI). Twenty seven out of 34 strains in this cluster caused BSI. These strains were all positive for collagen adhesion gene (cna) and have mutations in the locus, those were selected by Random Forest analysis. Univariate and multivariate analysis revealed that these gene mutations were the predictor for the incidence of BSI. Interestingly, mutant CNA protein showed lower attachment ability to collagen, suggesting that the mutant protein might contribute to the dissemination of bacteria.
Conclusions: These findings suggest that the bacterial genotype affects the clinical characteristics of MRSA infection. (c) 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. en-copyright= kn-copyright= en-aut-name=IwataYasunori en-aut-sei=Iwata en-aut-mei=Yasunori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SatouKenji en-aut-sei=Satou en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=FuruichiKengo en-aut-sei=Furuichi en-aut-mei=Kengo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YonedaIkuko en-aut-sei=Yoneda en-aut-mei=Ikuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MatsumuraTakuhiro en-aut-sei=Matsumura en-aut-mei=Takuhiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=YutaniMasahiro en-aut-sei=Yutani en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=FujinagaYukako en-aut-sei=Fujinaga en-aut-mei=Yukako kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=HaseAtsushi en-aut-sei=Hase en-aut-mei=Atsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=MoritaHidetoshi en-aut-sei=Morita en-aut-mei=Hidetoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=OhtaToshiko en-aut-sei=Ohta en-aut-mei=Toshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=SendaYasuko en-aut-sei=Senda en-aut-mei=Yasuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=Sakai-TakemoriYukiko en-aut-sei=Sakai-Takemori en-aut-mei=Yukiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=WadaTaizo en-aut-sei=Wada en-aut-mei=Taizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=FujitaShinichi en-aut-sei=Fujita en-aut-mei=Shinichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=MiyakeTaito en-aut-sei=Miyake en-aut-mei=Taito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=YasudaHaruka en-aut-sei=Yasuda en-aut-mei=Haruka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=SakaiNorihiko en-aut-sei=Sakai en-aut-mei=Norihiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=KitajimaShinji en-aut-sei=Kitajima en-aut-mei=Shinji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=ToyamaTadashi en-aut-sei=Toyama en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=ShinozakiYasuyuki en-aut-sei=Shinozaki en-aut-mei=Yasuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= en-aut-name=SagaraAkihiro en-aut-sei=Sagara en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=21 ORCID= en-aut-name=MiyagawaTaro en-aut-sei=Miyagawa en-aut-mei=Taro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=22 ORCID= en-aut-name=HaraAkinori en-aut-sei=Hara en-aut-mei=Akinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=23 ORCID= en-aut-name=ShimizuMiho en-aut-sei=Shimizu en-aut-mei=Miho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=24 ORCID= en-aut-name=KamikawaYasutaka en-aut-sei=Kamikawa en-aut-mei=Yasutaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=25 ORCID= en-aut-name=IkeoKazuho en-aut-sei=Ikeo en-aut-mei=Kazuho kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=26 ORCID= en-aut-name=ShichinoShigeyuki en-aut-sei=Shichino en-aut-mei=Shigeyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=27 ORCID= en-aut-name=UehaSatoshi en-aut-sei=Ueha en-aut-mei=Satoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=28 ORCID= en-aut-name=NakajimaTakuya en-aut-sei=Nakajima en-aut-mei=Takuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=29 ORCID= en-aut-name=MatsushimaKouji en-aut-sei=Matsushima en-aut-mei=Kouji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=30 ORCID= en-aut-name=KanekoShuichi en-aut-sei=Kaneko en-aut-mei=Shuichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=31 ORCID= en-aut-name=WadaTakashi en-aut-sei=Wada en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=32 ORCID= affil-num=1 en-affil=Division of Infection Control, Kanazawa University kn-affil= affil-num=2 en-affil=Faculty of Electrical and Computer Engineering, Kanazawa University kn-affil= affil-num=3 en-affil=Division of Nephrology, Kanazawa Medical University School of Medicine kn-affil= affil-num=4 en-affil=Division of Nephrology, Kanazawa University kn-affil= affil-num=5 en-affil=Department of Bacteriology, Kanazawa University kn-affil= affil-num=6 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=7 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=8 en-affil=Faculty of Electrical and Computer Engineering, Kanazawa University kn-affil= affil-num=9 en-affil=Graduate School of Environmental and Life Science, Okayama University kn-affil= affil-num=10 en-affil=University of Tsukuba kn-affil= affil-num=11 en-affil=Division of Infection Control, Kanazawa University kn-affil= affil-num=12 en-affil=Division of Infection Control, Kanazawa University kn-affil= affil-num=13 en-affil=Division of Infection Control, Kanazawa University kn-affil= affil-num=14 en-affil=Division of Infection Control, Kanazawa University kn-affil= affil-num=15 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=16 en-affil=Department of Nephrology and Laboratory Medicine, Kanazawa University kn-affil= affil-num=17 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Division of Blood Purification, Kanazawa University kn-affil= affil-num=18 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=19 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=20 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=21 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=22 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=23 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=24 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=25 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=26 en-affil=Laboratory of DNA Data Analysis, National Institute of Genetics kn-affil= affil-num=27 en-affil=Department of Molecular Preventive Medicine, University of Tokyo kn-affil= affil-num=28 en-affil=Department of Molecular Preventive Medicine, University of Tokyo kn-affil= affil-num=29 en-affil=Department of Molecular Preventive Medicine, University of Tokyo kn-affil= affil-num=30 en-affil=Department of Molecular Preventive Medicine, University of Tokyo kn-affil= affil-num=31 en-affil=epartment of Disease Control and Homeostasis, Kanazawa University kn-affil= affil-num=32 en-affil=Division of Nephrology, Kanazawa University, Kanazawa, Japan; Department of Nephrology and Laboratory Medicine, Kanazawa University kn-affil= en-keyword=Bloodstream infection kn-keyword=Bloodstream infection en-keyword=Cna kn-keyword=Cna en-keyword=MRSA kn-keyword=MRSA en-keyword=Whole genome sequencing kn-keyword=Whole genome sequencing END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190828 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ethyl acetate extract of Ceiba pentandra (L.) Gaertn. reduces methotrexate-induced renal damage in rats via antioxidant, anti-inflammatory, and antiapoptotic actions en-subtitle= kn-subtitle= en-abstract= kn-abstract= Methotrexate (MTX) is a chemotherapeutic agent and an immunosuppressant used to treat cancer and autoimmune diseases. However, its use is limited by its multi-organ toxicity, including nephrotoxicity, which is related to MTX-driven oxidative stress. Silencing oxidative stressors is therefore an important strategy in minimizing MTX adverse effects.Medicinal plants rich in phenolic compounds are probable candidates to overcome these oxidants. Herein, C. pentandra ethyl acetate extract showed powerful in?vitro radical-scavenging potential (IC50?=?0.0716) comparable to those of the standard natural (ascorbic acid, IC50?=?0.045) and synthetic (BHA, IC50?=?0.056) antioxidants. The effect of C. pentandra ethyl acetate extract against MTX-induced nephrotoxicity in rats was evaluated by administering the extract (400?mg/kg/day) or the standard antioxidant silymarin (100?mg/kg/day) orally for 5 days before and 5 days after a single MTX injection (20?mg/kg, i.p.).C.?pentandra showed slight superiorities over silymarin in restoring the MTX-impaired renal functions, with approximately twofold decreases in overall kidney function tests. C.?pentandra also improved renal antioxidant capacity and reduced the MTX-induced oxidative stress. Moreover, C.?pentandra inhibited MTX-initiated apoptotic and inflammatory cascades, and attenuated MTX-induced histopathological changes in renal tissue architecture.Phytochemical investigation of the extract led to the purification of the phenolics quercitrin (1), cinchonains 1a (2) and 1b (3), cis-clovamide (4), trans-clovamide (5), and glochidioboside (6); a structurally similar with many of the reported antioxidant and nephroprotective agents. In conclusion, these data demonstrate that C.?pentandra exhibits nephroprotective effect against MTX-induced kidney damage via its antioxidant, antiapoptotic and anti-inflammatory mechanisms. TaxonomyFunctional Disorder, Traditional Medicine, Herbal Medicine. en-copyright= kn-copyright= en-aut-name=Abouelela Mohamed E. en-aut-sei=Abouelela en-aut-mei= Mohamed E. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=Orabi Mohamed A.A. en-aut-sei=Orabi en-aut-mei= Mohamed A.A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=Abdelhamid Reda A. en-aut-sei=Abdelhamid en-aut-mei= Reda A. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Abdelkader Mohamed S. en-aut-sei=Abdelkader en-aut-mei= Mohamed S. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=Madkor Hafez R. en-aut-sei=Madkor en-aut-mei= Hafez R. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=Darwish Faten M.M. en-aut-sei=Darwish en-aut-mei= Faten M.M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=Hatano Tsutomu en-aut-sei=Hatano en-aut-mei= Tsutomu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=Elsadek Bakheet E.M. en-aut-sei=Elsadek en-aut-mei= Bakheet E.M. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil=Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University kn-affil= affil-num=2 en-affil=Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University kn-affil= affil-num=3 en-affil=Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University kn-affil= affil-num=4 en-affil=Department of Pharmacognosy, Faculty of Pharmacy, Sohag University kn-affil= affil-num=5 en-affil=Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University kn-affil= affil-num=6 en-affil=Department of Pharmacognosy, Faculty of Pharmacy, Assiut University kn-affil= affil-num=7 en-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University kn-affil= affil-num=8 en-affil=Department of Biochemistry, Faculty of Pharmacy, Al-Azhar University kn-affil= en-keyword=Ceiba pentandra(L.) Gaertn. kn-keyword=Ceiba pentandra(L.) Gaertn. en-keyword=Methotrexate kn-keyword=Methotrexate en-keyword=Nephrotoxicity kn-keyword=Nephrotoxicity en-keyword=Antioxidant kn-keyword=Antioxidant END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=4 article-no= start-page=315 end-page=323 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Construction and Characterization of a PGN_0297 Mutant of Porphyromonas gingivalis: Evidence of the Contribution of PGN_0297 to Gingipain Activity en-subtitle= kn-subtitle= en-abstract= kn-abstract=The periodontal pathogen Porphyromonas gingivalis shows colonial pigmentation on blood agar and produces gingipains (Kgp, RgpA, and RgpB), cysteine proteases involved in an organism’s virulence and pigmentation. We showed previously that deletion of the PGN_0300 gene abolished the pigmentation activity and reduced the proteolytic activity of gingipains. The role of the PGN_0297 gene, which consists of an operon with the PGN_0300 gene, is unclear. Herein we examined the effect of PGN_0297 gene deletion on the pigmentation and proteolytic activities and transcriptional levels of gingipains. A PGN_0297 gene deletion mutant (ΔPGN_0297) did not exhibit the pigmentation. The proteolytic activity of the gingipains was decreased in the culture supernatant and on the cell surface of ΔPGN_0297. The mutant ΔPGN_0297 failed to attenuate Akt phosphorylation at Thr308 and Ser473, but both phosphorylations were attenuated in the wild-type and its complementation strain. The deletion of PGN_0297 gene did not substantially affect the transcriptional levels of the gingipain genes kgp, rgpA, and rgpB. Taken together, these results indicate that PGN_0297 is closely involved in the secretion and maturation of gingipains. en-copyright= kn-copyright= en-aut-name=OnoShintaro en-aut-sei=Ono en-aut-mei=Shintaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakayamaMasaaki en-aut-sei=Nakayama en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TachibanaMasato en-aut-sei=Tachibana en-aut-mei=Masato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=Abu Saleh Muhammad Shahriar en-aut-sei=Abu Saleh Muhammad Shahriar en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HelingWang en-aut-sei=Heling en-aut-mei=Wang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TakashibaShogo en-aut-sei=Takashiba en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OharaNaoya en-aut-sei=Ohara en-aut-mei=Naoya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=2 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=3 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=4 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=5 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=6 en-affil=Department of Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=7 en-affil=Department of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=periodontitis kn-keyword=periodontitis en-keyword=Porphyromonas gingivalis kn-keyword=Porphyromonas gingivalis en-keyword=gingipain kn-keyword=gingipain en-keyword=C-terminal domain kn-keyword=C-terminal domain en-keyword=secretion system kn-keyword=secretion system END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=4 article-no= start-page=611 end-page=633 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=20190320 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Method to Suppress Isobaric and Polyatomic Interferences for Measurements of Highly Siderophile Elements in Desilicified Geological Samples en-subtitle= kn-subtitle= en-abstract= kn-abstract= Sample decomposition using inverse aqua regia at elevated temperatures and pressures (e.g., Carius tube or high‐pressure asher) is the most common method used to extract highly siderophile elements (HSEs: Ru, Rh, Pd, Re, Os, Ir, Pt and Au) from geological samples. Recently, it has been recognised that additional HF desilicification is necessary to better recover HSEs, potentially contained within silicate or oxide minerals in mafic samples, which cannot be dissolved solely by inverse aqua regia. However, the abundance of interfering elements tends to increase in the eluent when conventional ion‐exchange purification procedures are applied to desilicified samples. In this study, we developed an improved purification method to determine HSEs in desilicified samples. This method enables the reduction of the ratios of isobaric and polyatomic interferences, relative to the measured intensities of HSE isotope masses, to less than a few hundred parts per million. Furthermore, the total procedural blanks are either comparable to or lower than conventional methods. Thus, this method allows accurate and precise HSE measurements in mafic and ultramafic geological samples, without the need for interference corrections. Moreover, the problem of increased interfering elements, such as Zr for Pd and Cr for Ru, is circumvented for the desilicified samples. en-copyright= kn-copyright= en-aut-name=ZhouXiaoyu en-aut-sei=Zhou en-aut-mei=Xiaoyu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TanakaRyoji en-aut-sei=Tanaka en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YamanakaMasahiro en-aut-sei=Yamanaka en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SakaguchiChie en-aut-sei=Sakaguchi en-aut-mei=Chie kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=NakamuraEizo en-aut-sei=Nakamura en-aut-mei=Eizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=2 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=3 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=4 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= affil-num=5 en-affil=The Pheasant Memorial Laboratory for Geochemistry and Cosmochemistry, Institute for Planetary Materials, Okayama University kn-affil= en-keyword=highly siderophile elements kn-keyword=highly siderophile elements en-keyword=desilicification kn-keyword=desilicification en-keyword=isotope dilution method kn-keyword=isotope dilution method en-keyword=high resolution ICP-MS kn-keyword=high resolution ICP-MS en-keyword=N-TIMS kn-keyword=N-TIMS END start-ver=1.4 cd-journal=joma no-vol=73 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2019 dt-pub=201902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=The Role of Kallikrein-Related Peptidases in Atopic Dermatitis en-subtitle= kn-subtitle= en-abstract= kn-abstract= Excessive protease activity is a characteristic abnormality that affects the epidermal barrier in patients with atopic dermatitis (AD). Kallikrein-related peptidases (KLKs) are excessively expressed in AD lesions, and it is suggested that the abnormal action of KLKs is involved in the skin barrier dysfunction in AD. In other words, overexpressed KLKs disrupt the normal barrier function, and due to that breakdown, external substances that can become antigens of AD easily invade the epidermis, resulting in dermatitis, coupled with the induction of Th2 cytokines. Further investigations are required to elucidate the role of KLKs in AD; this knowledge could contribute to the design of new therapeutic and prophylactic drugs for AD. en-copyright= kn-copyright= en-aut-name=MorizaneShin en-aut-sei=Morizane en-aut-mei=Shin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil=Department of Dermatology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= en-keyword=atopic dermatitis kn-keyword=atopic dermatitis en-keyword=kallikrein-related peptidases kn-keyword=kallikrein-related peptidases en-keyword=epidermal barrier dysfunction kn-keyword=epidermal barrier dysfunction END start-ver=1.4 cd-journal=joma no-vol=292 cd-vols= no-issue=20 article-no= start-page=8436 end-page=8446 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=201705 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Metformin directly binds the alarmin HMGB1 and inhibits its proinflammatory activity en-subtitle= kn-subtitle= en-abstract= kn-abstract= Metformin is the first-line drug in the treatment of type 2 diabetes. In addition to its hypoglycemic effect, metformin has an anti-inflammatory function, but the precise mechanism promoting this activity remains unclear. High mobility group box 1 (HMGB1) is an alarmin that is released from necrotic cells and induces inflammatory responses by its cytokine-like activity and is, therefore, a target of anti-inflammatory therapies. Here we identified HMGB1 as a novel metformin-binding protein by affinity purification using a biotinylated metformin analogue. Metformin directly bound to the C-terminal acidic tail of HMGB1. Both in vitro and in vivo, metformin inhibited inflammatory responses induced by full-length HMGB1 but not by HMGB1 lacking the acidic tail. In an acetaminophen-induced acute liver injury model in which HMGB1 released from injured cells exacerbates the initial injury, metformin effectively reduced liver injury and had no additional inhibitory effects when the extracellular HMGB1 was blocked by anti-HMGB1-neutralizing antibody. In summary, we report for the first time that metformin suppresses inflammation by inhibiting the extracellular activity of HMGB1. Because HMGB1 plays a major role in inflammation, our results suggest possible new ways to manage HMGB1-induced inflammation. en-copyright= kn-copyright= en-aut-name=HoriuchiTakahiro en-aut-sei=Horiuchi en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SakataNatsumi en-aut-sei=Sakata en-aut-mei=Natsumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NarumiYoshihiro en-aut-sei=Narumi en-aut-mei=Yoshihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraTomohiro en-aut-sei=Kimura en-aut-mei=Tomohiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HayashiTakashi en-aut-sei=Hayashi en-aut-mei=Takashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NaganoKeisuke en-aut-sei=Nagano en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=LiuKeyue en-aut-sei=Liu en-aut-mei=Keyue kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NishiboriMasahiro en-aut-sei=Nishibori en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TsukitaSohei en-aut-sei=Tsukita en-aut-mei=Sohei kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=YamadaTetsuya en-aut-sei=Yamada en-aut-mei=Tetsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=KatagiriHideki en-aut-sei=Katagiri en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=ShirakawaRyutaro en-aut-sei=Shirakawa en-aut-mei=Ryutaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=HoriuchiHisanori en-aut-sei=Horiuchi en-aut-mei=Hisanori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= affil-num=1 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= affil-num=2 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= affil-num=3 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= affil-num=4 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= affil-num=5 en-affil= Biomedical Technology Research Center, Tokushima Research Institute kn-affil= affil-num=6 en-affil=First Institute of New Drug Discovery, Otsuka Pharmaceutical Co kn-affil= affil-num=7 en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=8 en-affil=Department of Pharmacology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences kn-affil= affil-num=9 en-affil=Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine kn-affil= affil-num=10 en-affil=Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine kn-affil= affil-num=11 en-affil=Department of Metabolism and Diabetes, Tohoku University Graduate School of Medicine kn-affil= affil-num=12 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= affil-num=13 en-affil=Department of Molecular and Cellular Biology, Institute of Development, Aging and Cancer, Tohoku University kn-affil= en-keyword=cytokine kn-keyword=cytokine en-keyword=inflammation kn-keyword=inflammation en-keyword=liver injury kn-keyword=liver injury en-keyword=metformin kn-keyword=metformin en-keyword=p38 MAPK kn-keyword=p38 MAPK END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue= article-no= start-page=5 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2017 dt-pub=20170201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization en-subtitle= kn-subtitle= en-abstract=　銀杏種子から高分子量 （SDS-PAGE で120 kDa） を有し，α-フコース含有オリゴ糖に活性を示すα-フコシダーゼ（α-fucosidase Gb）を均一に精製した．ルイス a エピトープ含有 N-グリカンを基質とした場合，α-fucosidase Gb の至適 pH は 5.5 付近であることから，本酵素は液胞のような酸性環境で機能していることが示唆された．N?末端アミノ酸配列が化学修飾のため同定できなかったため，本酵素が GH29 ファミリーに属するかどうかは不明である．α-Fucosidase Gb は，Lacto-N-fucopentaose IIIの α1,3-フコース残基やルイス a エピトープ含有の植物複合型N-グリカンのα1,4-フコース残基を加水分解することから，α-フコース含有オリゴ糖やN 型糖タンパク質の分解プロセスに関与することが示唆された． kn-abstract=　We have identified, and purified to homogeneity, a high molecular weight Ginkgo biloba α-fucosidase (α-fucosidase Gb, 120 kDa estimated by SDS?PAGE) with activity against α-fucosylated oligosaccharides. When a Lewis a epitope-containing N-glycan was used as a substrate, α-fucosidase Gb showed optimum activity at approximately pH 5.5, suggesting that it functions in acidic environments such as the vacuole. It remains uncertain, however, whether this Ginkgo α-fucosidase belongs to the GH29 family, since its N-terminal sequence could not be determined, probably due to a chemical modification. α-Fucosidase Gb showed substantial activity towards the α1,3-fucosyl linkage in Lacto-N-fucopentaose III and an α1,4-fucosyl linkage in the Lewis a epitope found in plant complex type N-glycans, indicating an involvement in the degradation process of α-fucosylated oligosaccharides or N-glycoproteins. en-copyright= kn-copyright= en-aut-name=ItanoSatsuki en-aut-sei=Itano en-aut-mei=Satsuki kn-aut-name=板野紗月 kn-aut-sei=板野 kn-aut-mei=紗月 aut-affil-num=1 ORCID= en-aut-name=MaedaMegumi en-aut-sei=Maeda en-aut-mei=Megumi kn-aut-name=前田恵 kn-aut-sei=前田 kn-aut-mei=恵 aut-affil-num=2 ORCID= en-aut-name=Md. Ziaur Rahman en-aut-sei=Md. Ziaur Rahman en-aut-mei= kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= affil-num=1 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=2 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 affil-num=3 en-affil=Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission kn-affil= affil-num=4 en-affil=Graduate School of Environmental and life Science, Okayama University kn-affil=岡山大学大学院環境生命科学研究科 en-keyword=α-fucosidase kn-keyword=α-fucosidase en-keyword=plant N-glycan kn-keyword=plant N-glycan en-keyword=N-glycan degradation kn-keyword=N-glycan degradation en-keyword=Ginkgo biloba kn-keyword=Ginkgo biloba END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue= article-no= start-page=19 end-page=22 dt-received= dt-revised= dt-accepted= dt-pub-year=2014 dt-pub=201402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Evaluation of Tree Barks and their Hybrid Materials with Hydrophilic Polymers as Adsorbents for Heavy Metal Ions kn-title=スギ，ヒノキ樹皮及び樹皮?透水性ポリマー複合材料の重金属イオン吸着能評価 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The radioactive pollutions in environment caused by the accident of atomic power plants in Fukushima, Japan, is still serious and rapid measures to the severe problem is required. It is well known that various biomass have adsorption ability for toxic metal ions from aqueous media. We have evaluated the adsorption ability of the bark of Cryptomeria japonica and Chamaecyparis obtusa, and their hybrid materials with hydrophilic polymers, for cesium, strontium, and other heavy metal ions. As the results, these materials are useful as adsorbents for a water and soil purification system. en-copyright= kn-copyright= en-aut-name=IshikawaTeruhiko en-aut-sei=Ishikawa en-aut-mei=Teruhiko kn-aut-name=石川彰彦 kn-aut-sei=石川 kn-aut-mei=彰彦 aut-affil-num=1 ORCID= en-aut-name=KatayamaShyota en-aut-sei=Katayama en-aut-mei=Shyota kn-aut-name=片山翔太 kn-aut-sei=片山 kn-aut-mei=翔太 aut-affil-num=2 ORCID= en-aut-name=KitaokaTatsuya en-aut-sei=Kitaoka en-aut-mei=Tatsuya kn-aut-name=北岡達也 kn-aut-sei=北岡 kn-aut-mei=達也 aut-affil-num=3 ORCID= en-aut-name=IgamiShinya en-aut-sei=Igami en-aut-mei=Shinya kn-aut-name=居上真也 kn-aut-sei=居上 kn-aut-mei=真也 aut-affil-num=4 ORCID= en-aut-name=ItoKatsuhito en-aut-sei=Ito en-aut-mei=Katsuhito kn-aut-name=伊藤勝仁 kn-aut-sei=伊藤 kn-aut-mei=勝仁 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院教育学研究科 affil-num=2 en-affil= kn-affil=岡山大学大学院教育学研究科 affil-num=3 en-affil= kn-affil=岡山大学大学院教育学研究科 affil-num=4 en-affil= kn-affil=岡山大学大学院教育学研究科 affil-num=5 en-affil= kn-affil=株式会社アイセロ en-keyword=cesium kn-keyword=cesium en-keyword=strontium kn-keyword=strontium en-keyword=metal ion kn-keyword=metal ion en-keyword=adsorption kn-keyword=adsorption en-keyword=radioactivity kn-keyword=radioactivity en-keyword=tree barks kn-keyword=tree barks END start-ver=1.4 cd-journal=joma no-vol=28 cd-vols= no-issue=4 article-no= start-page=1633 end-page=1639 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201204 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=An extracellular serine protease produced by Vibrio vulnificus NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel en-subtitle= kn-subtitle= en-abstract= kn-abstract=Vibrio vulnificus is a ubiquitous estuarine microorganism but causes fatal systemic infections in immunocompromised humans, cultured eels or shrimps. An extracellular metalloprotease VVP/VvpE has been reported to be a potential virulence factor of the bacterium; however, a few strains isolated from a diseased eel or shrimp were recently found to produce a serine protease termed VvsA, but not VVP/VvpE. In the present study, we found that these strains had lost the 80 kb genomic region including the gene encoding VVP/VvpE. We also purified VvsA from the culture supernatant through ammonium sulfate fractionation, gel filtration and ion-exchange column chromatography, and the enzyme was demonstrated to be a chymotrypsin-like protease, as well as those from some vibrios. The gene vvsA was shown to constitute an operon with a downstream gene vvsB, and several Vibrio species were found to have orthologues of vvsAB. These findings indicate that the genes vvp/vvpE and vvsAB might be mobile genetic elements. en-copyright= kn-copyright= en-aut-name=MiyoshiShin-ichi en-aut-sei=Miyoshi en-aut-mei=Shin-ichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=JiyouWang en-aut-sei=Jiyou en-aut-mei=Wang kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KatohKeizo en-aut-sei=Katoh en-aut-mei=Keizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=SenohMitsutoshi en-aut-sei=Senoh en-aut-mei=Mitsutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=MizunoTamaki en-aut-sei=Mizuno en-aut-mei=Tamaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MaeharaYoko en-aut-sei=Maehara en-aut-mei=Yoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci affil-num=2 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci affil-num=3 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci affil-num=4 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci affil-num=5 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci affil-num=6 en-affil= kn-affil=Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci en-keyword=Polymerase chain reaction kn-keyword=Polymerase chain reaction en-keyword=Purification kn-keyword=Purification en-keyword=Serine protease kn-keyword=Serine protease en-keyword=Metalloprotease kn-keyword=Metalloprotease en-keyword=Vibrio vulnificus kn-keyword=Vibrio vulnificus END start-ver=1.4 cd-journal=joma no-vol=19 cd-vols= no-issue=2 article-no= start-page=77 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=1986 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=UPTAKE OF NITROGEN AND PHOSPHATE, AND WATER PURIFICATION CAPACITY BY WATER HYACINTH (Eichhornia crassipes (Mart.) Solms) en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=AoyamaIsao en-aut-sei=Aoyama en-aut-mei=Isao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NishizakiHisao en-aut-sei=Nishizaki en-aut-mei=Hisao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YagiMasakazu en-aut-sei=Yagi en-aut-mei=Masakazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=17 cd-vols= no-issue=3 article-no= start-page=111 end-page=121 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=1978 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=α-GLUCOSIDASE FROM MUCOR JAVANICUS I. Purification and Crystallization en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MiyakeToshio en-aut-sei=Miyake en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SuzukiYukio en-aut-sei=Suzuki en-aut-mei=Yukio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= END start-ver=1.4 cd-journal=joma no-vol=61 cd-vols= no-issue=11 article-no= start-page=2823 end-page=2832 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201211 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Vaspin Is an Adipokine Ameliorating ER Stress in Obesity as a Ligand for Cell-Surface GRP78/MTJ-1 Complex en-subtitle= kn-subtitle= en-abstract= kn-abstract=It is unknown whether adipokines derived from adipose tissues modulate endoplasmic reticulum (ER) stress induced in obesity. Here, we show that visceral adipose tissue-derived serine protease inhibitor (vaspin) binds to cell-surface 78-kDa glucose-regulated protein (GRP78), which is recruited from ER to plasma membrane under ER stress. Vaspin transgenic mice were protected from diet-induced obesity, glucose intolerance, and hepatic steatosis, while vaspin-deficient mice developed glucose intolerance associated with upregulation of ER stress markers. With tandem affinity tag purification using HepG2 cells, we identified GRP78 as an interacting molecule. The complex formation of vaspin, GRP78, and murine tumor cell DnaJ-like protein 1 (MTJ-1) (DnaJ homolog, subfamily C, member 1) on plasma membrane was confirmed by cell-surface labeling with biotin and immunoprecipitation in liver tissues and H-4-II-E-C3 cells. The addition of recombinant human vaspin in the cultured H-4-II-E-C3 cells also increased the phosphorylation of Akt and AMP-activated protein kinase (AMPK) in a dose-dependent manner, and anti-GRP78 antibodies completely abrogated the vaspin-induced upregulation of pAkt and pAMPK Vaspin is a novel ligand for cell-surface GRP78/MTJ-1 complex, and its subsequent signals exert beneficial effects on ER stress-induced metabolic dysfunctions. Diabetes 61:2823-2832, 2012 en-copyright= kn-copyright= en-aut-name=NakatsukaAtsuko en-aut-sei=Nakatsuka en-aut-mei=Atsuko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WadaJun en-aut-sei=Wada en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=IsedaIzumi en-aut-sei=Iseda en-aut-mei=Izumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=TeshigawaraSanae en-aut-sei=Teshigawara en-aut-mei=Sanae kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HigashioKanji en-aut-sei=Higashio en-aut-mei=Kanji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiKazutoshi en-aut-sei=Murakami en-aut-mei=Kazutoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=KanzakiMotoko en-aut-sei=Kanzaki en-aut-mei=Motoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=InoueKentaro en-aut-sei=Inoue en-aut-mei=Kentaro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=TeramiTakahiro en-aut-sei=Terami en-aut-mei=Takahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=KatayamaAkihiro en-aut-sei=Katayama en-aut-mei=Akihiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= en-aut-name=HidaKazuyuki en-aut-sei=Hida en-aut-mei=Kazuyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=11 ORCID= en-aut-name=EguchiJun en-aut-sei=Eguchi en-aut-mei=Jun kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=12 ORCID= en-aut-name=HoriguchiChikage Sato en-aut-sei=Horiguchi en-aut-mei=Chikage Sato kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=13 ORCID= en-aut-name=OgawaDaisuke en-aut-sei=Ogawa en-aut-mei=Daisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=14 ORCID= en-aut-name=MatsukiYasushi en-aut-sei=Matsuki en-aut-mei=Yasushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=15 ORCID= en-aut-name=HiramatsuRyuji en-aut-sei=Hiramatsu en-aut-mei=Ryuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=16 ORCID= en-aut-name=YagitaHideo en-aut-sei=Yagita en-aut-mei=Hideo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=17 ORCID= en-aut-name=KakutaShigeru en-aut-sei=Kakuta en-aut-mei=Shigeru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=18 ORCID= en-aut-name=IwakuraYoichiro en-aut-sei=Iwakura en-aut-mei=Yoichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=19 ORCID= en-aut-name=MakinoHirofumi en-aut-sei=Makino en-aut-mei=Hirofumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=20 ORCID= affil-num=1 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=2 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=3 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=4 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=5 en-affil= kn-affil=Metabolome Pharmaceut Inc affil-num=6 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=7 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=8 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=9 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=10 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=11 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci, Okayama affil-num=12 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci affil-num=13 en-affil= kn-affil=Okayama Univ, Dept Diabet Nephropathy, Grad Sch Med Dent & Pharmaceut Sci affil-num=14 en-affil= kn-affil=Okayama Univ, Dept Diabet Nephropathy, Grad Sch Med Dent & Pharmaceut Sci affil-num=15 en-affil= kn-affil=Dainippon Sumitomo Pharma, Genom Sci Labs affil-num=16 en-affil= kn-affil=Dainippon Sumitomo Pharma, Genom Sci Labs affil-num=17 en-affil= kn-affil=Juntendo Univ, Sch Med, Dept Immunol affil-num=18 en-affil= kn-affil=Univ Tokyo, Inst Med Sci, Ctr Expt Med & Syst Biol affil-num=19 en-affil= kn-affil=Univ Tokyo, Inst Med Sci, Ctr Expt Med & Syst Biol affil-num=20 en-affil= kn-affil=Okayama Univ, Dept Med & Clin Sci, Grad Sch Med Dent & Pharmaceut Sci END start-ver=1.4 cd-journal=joma no-vol=34 cd-vols= no-issue= article-no= start-page=20 end-page=24 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=201212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Evaluation of Fiber Crops as Adsorption Materials for Cesium and Strontium Ions kn-title=植物繊維類のセシウム及びストロンチウムイオン吸着能評価 en-subtitle= kn-subtitle= en-abstract= kn-abstract=After the accident of atomic power plants in Fukushima, Japan, the removal of radioactive materials released to environment is most important task and rapid response to the severe problem is required. It is well known that various biomass have adsorption ability for toxic metal ions from aqueous media. We have evaluated the adsorption ability of many fiber crops for cesium and strontium ions. As the results, several fiber crops have considerable ability for the adsorption of such metal ions and are useful for a water and soil purification system in large scale. en-copyright= kn-copyright= en-aut-name=IshikawaTeruhiko en-aut-sei=Ishikawa en-aut-mei=Teruhiko kn-aut-name=石川彰彦 kn-aut-sei=石川 kn-aut-mei=彰彦 aut-affil-num=1 ORCID= en-aut-name=IshimotoAya en-aut-sei=Ishimoto en-aut-mei=Aya kn-aut-name=石本彩 kn-aut-sei=石本 kn-aut-mei=彩 aut-affil-num=2 ORCID= en-aut-name=MizushimaShiho en-aut-sei=Mizushima en-aut-mei=Shiho kn-aut-name=水島志穂 kn-aut-sei=水島 kn-aut-mei=志穂 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院教育学研究科 affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil= en-keyword=cesium kn-keyword=cesium en-keyword=strontium kn-keyword=strontium en-keyword=adsorption kn-keyword=adsorption en-keyword=radioactivity kn-keyword=radioactivity en-keyword=fiber crops kn-keyword=fiber crops END start-ver=1.4 cd-journal=joma no-vol=684 cd-vols= no-issue= article-no= start-page=41 end-page=51 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=2011 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and Crystallization of Oxygen-Evolving Photosystem II Core Complex from Thermophilic Cyanobacteria en-subtitle= kn-subtitle= en-abstract= kn-abstract=This chapter describes the purification and crystallization of oxygen-evolving photosystem II core dimer complex from a thermophilic cyanobacterium Thermosynechococcus vulcanus. Procedures used for purification of photosystem II from the cyanobacterium involves cultivation of cells, isolation of thylakoid membranes, purification of crude and pure photosystem II core complexes by detergent solubilization, followed by differential centrifugation and column chromatography. The purified core dimer particles were successfully used for crystallization, and the methods and conditions used for crystallization are presented. These purification and crystallization procedures can be applied for another thermophilic cyanobacterium T. elongatus. en-copyright= kn-copyright= en-aut-name=ShenJian-Ren en-aut-sei=Shen en-aut-mei=Jian-Ren kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KawakamiKeisuke en-aut-sei=Kawakami en-aut-mei=Keisuke kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KoikeHiroyuki en-aut-sei=Koike en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=2 en-affil= kn-affil=Graduate School of Natural Science and Technology, Okayama University affil-num=3 en-affil= kn-affil=Department of Biosciences, Faculty of Science and Engineering, Chuo University en-keyword=Photosystem II kn-keyword=Photosystem II en-keyword=Oxygen evolution kn-keyword=Oxygen evolution en-keyword=Crystallization kn-keyword=Crystallization en-keyword=Membrane proteins kn-keyword=Membrane proteins en-keyword=Ion-exchange chromatography kn-keyword=Ion-exchange chromatography END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=2 article-no= start-page=137 end-page=143 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study for the structures of the HA complexes produced by Clostridium botulinum type A mutant strain kn-title=ボツリヌスＡ型菌変異株が産生するHA複合体の構造に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Clostridium botulinum produces seven neurotoxin (NTX) serotypes, classified from as A to G. In culture, NTX forms protein complexes by association with non-toxic components, such as nontoxic-nonhemagglutinin (NTNH) and hemagglutinins (HA1, HA2, HA3). C. botulinum serotype A produces three types of toxin complexes, M-toxin (NTX and NTNH), L-toxin (M-toxin and HAs) and LL-toxin (dimer of L-toxin). In this study, I found three HA complexes in the culture of a nontoxigenic mutant serotype A lacking ntx and ntnh expression. The HA complexes displayed similar banding patterns on SDS-PAGE, but the staining intensities of the HA1 and HA2 bands were different. In addition, their native-PAGE banding profiles exhibited different behaviors. The large-molecular-size HA complex showed the highest activity, similar to that of an L-toxin. Based on the combined results of the PAGE and gel-filtration profiles, the differences in molecular size among the three HA complexes were thought to be caused by different numbers of HA1 and HA2 molecules. This paper reports for the first time the purification and characterization of a native HA complex of serotype A, and suggests that the HA can form complex structures without NTX and NTNH. This may help in understanding the toxin complex assembly pathway. en-copyright= kn-copyright= en-aut-name=MaShaobo en-aut-sei=Ma en-aut-mei=Shaobo kn-aut-name=馬少博 kn-aut-sei=馬 kn-aut-mei=少博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　病原細菌学 en-keyword=ボツリヌス毒素（botulinum toxin） kn-keyword=ボツリヌス毒素（botulinum toxin） en-keyword=ボツリヌス菌（Clostridium botulinum） kn-keyword=ボツリヌス菌（Clostridium botulinum） en-keyword=血球凝集素（hemagglutinin） kn-keyword=血球凝集素（hemagglutinin） en-keyword=タンパク質複合体構造（protein complex structure） kn-keyword=タンパク質複合体構造（protein complex structure） END start-ver=1.4 cd-journal=joma no-vol=124 cd-vols= no-issue=1 article-no= start-page=15 end-page=26 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120401 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Hypoglycemic activity of Momordica charantia (bitter melon) kn-title=ニガウリ抽出物の血糖降下作用に関する文献的考察 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Diabetes mellitus (DM) represents a global health and economical problem. Many patients with DM in Asia, South America, India and East Africa have traditionally used the water extract of unripe fruits of Momordica charantia (bitter melon) as some form of complementary and alternative medicine. Studies of laboratory animals have shown the beneficial blood-glucose lowering and anti-diabetic effects of this remedy. Some oral components that bring lower blood glucose level have been isolated : charantin (sterol glycosides), charantin (polypeptide) and cucurbine-type triterpenes. Part of their actions are related to AMP-activated kinase and repression of the oxidative stress that is increased in DM. Most clinical reports are not fully convincing due to the lack of randomized control studies. The present article reviews the pharmacological and clinical effects of bitter melon with special emphasis on the anti-diabetic effects, and some effects that would require caution in the context of human trials. en-copyright= kn-copyright= en-aut-name=MankuraMitsumasa en-aut-sei=Mankura en-aut-mei=Mitsumasa kn-aut-name=万倉三正 kn-aut-sei=万倉 kn-aut-mei=三正 aut-affil-num=1 ORCID= en-aut-name=NodaYasuko en-aut-sei=Noda en-aut-mei=Yasuko kn-aut-name=野田泰子 kn-aut-sei=野田 kn-aut-mei=泰子 aut-affil-num=2 ORCID= en-aut-name=MoriAkitane en-aut-sei=Mori en-aut-mei=Akitane kn-aut-name=森昭胤 kn-aut-sei=森 kn-aut-mei=昭胤 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　医療薬学・先端薬物療法開発学 affil-num=2 en-affil= kn-affil=岡山大学医学部　病原細菌学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=ニガウリ (bitter melon) kn-keyword=ニガウリ (bitter melon) en-keyword=Momordica charantia kn-keyword=Momordica charantia en-keyword=糖尿病 (diabetes mellitus) kn-keyword=糖尿病 (diabetes mellitus) en-keyword=酸化ストレス (oxidative stress) kn-keyword=酸化ストレス (oxidative stress) END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue= article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=2012 dt-pub=20120201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of Thermostable Amidase from Thermus sp.O-3-1 kn-title=好熱性細菌Thermus sp.O-3-1由来耐熱性アミダーゼの精製及び性質検討 en-subtitle= kn-subtitle= en-abstract=好熱性細菌Thermus sp.O-3-1 由来の耐熱性アミダーゼ遺伝子を大腸菌中にクローニングし，その塩基配列を決定した．ami 遺伝子は930 bp からなり，310アミノ酸をコードしていた．本酵素の分子量は33，089 Daであると予想された．Thermus sp.O-3-1 由来アミダーゼを大腸菌で生産させ，熱処理とDEAE-トヨパール650M陰イオン交換カラム等により精製した．ゲル濾過クロマトグラフィーとSDS-PAGE の結果から本酵素は分子質量33 kDa のサブユニット2分子からなるダイマー構造を有していることが明らかとなった．精製酵素の熱安定性は80℃まで，pH 安定性は7.0〜10.0であり，安定性の 高い酵素であった．最適温度は90℃，最適 pH は9.0であ った．EDTA により活性が著しく阻害され，Co(2+)やNi(2+)，Mn(2+)によって活性の回復，向上が見られたため，本酵素は金属酵素であることが示唆された．基質特異性の検討 の結果，L-Leu-pNA よりもD-Leu-pNA に対して高い活性を示したため，本酵素がＤ-アミノ酸基質に特異性を持つアミダーゼであることが判明した．本酵素は耐熱性を有するユニークなＤ-アミノ酸アミダーゼであり，今後産業利用が期待される． kn-abstract=The gene encoding a thermostable amidase (EC 3.5.1.4) from thermophilic bacterium Thermus sp.O-3-1, was cloned and expressed in Escherichia coli JM109. The cloned amidase gene (ami) is 930 bp and encodes a protein composed of 310 amino acids. The protein is predicted to have a molecular mass of 33,089 Da. The amidase from Thermus sp.O-3-1 was purified by heat treatment and DEAE Toyopearl 650M column chromatography. The molecular mass of the native enzyme was estimated to be about 70 kDa by gel filtration chromatography, indicating that the enzyme has a homodimeric structure. The purified enzyme was stable up to 80°C and within a pH range from 7.0 to 10.0. The optimum temperature and pH for enzyme activity were 90°C, and 9.0, respectively. The enzyme was strongly inhibited by the metal-chelating compound EDTA. The activity of the EDTA-treated enzyme was reactivated by the addition of Co(2+), Ni(2+) and Mn(2+) ions. Therefore the enzyme was predicted to be metalloenzyme. Finally, as a result of investigation into substrate specificity, the purified enzyme was suggested to be D-amino acid specific amidase, as it showed higher activity toward D-Leu-pNA than L-Leu-pNA. en-copyright= kn-copyright= en-aut-name=KobayashiFumiaki en-aut-sei=Kobayashi en-aut-mei=Fumiaki kn-aut-name=小林史明 kn-aut-sei=小林 kn-aut-mei=史明 aut-affil-num=1 ORCID= en-aut-name=AomineHiroki en-aut-sei=Aomine en-aut-mei=Hiroki kn-aut-name=青峰弘起 kn-aut-sei=青峰 kn-aut-mei=弘起 aut-affil-num=2 ORCID= en-aut-name=MizunashiWataru en-aut-sei=Mizunashi en-aut-mei=Wataru kn-aut-name=水無渉 kn-aut-sei=水無 kn-aut-mei=渉 aut-affil-num=3 ORCID= en-aut-name=YuFujio en-aut-sei=Yu en-aut-mei=Fujio kn-aut-name=湯不二夫 kn-aut-sei=湯 kn-aut-mei=不二夫 aut-affil-num=4 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=5 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil= affil-num=2 en-affil= kn-affil= affil-num=3 en-affil= kn-affil=(株)三菱レイヨン affil-num=4 en-affil= kn-affil= affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=amidase kn-keyword=amidase en-keyword=thermostable enzyme kn-keyword=thermostable enzyme en-keyword=Thermus kn-keyword=Thermus en-keyword=D-amino acid specific amidase kn-keyword=D-amino acid specific amidase END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue= article-no= start-page=3 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=2011 dt-pub=20110201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of l-Methionine Decarboxylase from Streptomyces sp. 590 kn-title=放線菌Streptomyces sp.590由来l-メチオニン脱炭酸酵素の精製および性質検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=L-Methionine decarboxylase [EC 4.1.1.57] catalyzes the decarboxylation of L-methionine and is a pyridoxal 5’-phosohate(PLP)-dependent enzyme. L-Methionine decarboxylase has been purified 630-fold by DEAE-Toyopearl 650M, Phenyl-Toyopearl 650M and Sephacryl S-300 column chromatographies from Streptomyces sp.590. The enzyme has a dimeric structure with identical subunits of Mr 60,000. This enzyme shows optimum activity at pH7.0 and 45°C, and is stable between pH5.7 and pH9.0. L-Methionine decarboxylase has antitumor activity against RERF-LC-AI and HeLa cells. Ten N-terminal amino acid sequence of L-methionine decarboxylase was determined, and the sequence showed no homology with other reported proteins. en-copyright= kn-copyright= en-aut-name=MaemuraTomomi en-aut-sei=Maemura en-aut-mei=Tomomi kn-aut-name=前村知美 kn-aut-sei=前村 kn-aut-mei=知美 aut-affil-num=1 ORCID= en-aut-name=UchitomiKumiko en-aut-sei=Uchitomi en-aut-mei=Kumiko kn-aut-name=内富久美子 kn-aut-sei=内富 kn-aut-mei=久美子 aut-affil-num=2 ORCID= en-aut-name=KusakaChika en-aut-sei=Kusaka en-aut-mei=Chika kn-aut-name=日下知香 kn-aut-sei=日下 kn-aut-mei=知香 aut-affil-num=3 ORCID= en-aut-name=InagakiJunko en-aut-sei=Inagaki en-aut-mei=Junko kn-aut-name=稲垣純子 kn-aut-sei=稲垣 kn-aut-mei=純子 aut-affil-num=4 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=5 ORCID= en-aut-name=SodaKenji en-aut-sei=Soda en-aut-mei=Kenji kn-aut-name=左右田健次 kn-aut-sei=左右田 kn-aut-mei=健次 aut-affil-num=6 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=農芸化学コース affil-num=2 en-affil= kn-affil=農芸化学コース affil-num=3 en-affil= kn-affil=農芸化学コース affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 affil-num=5 en-affil= kn-affil=農芸化学コース affil-num=6 en-affil= kn-affil=京都大学 affil-num=7 en-affil= kn-affil=農芸化学コース en-keyword=L-methionine decarboxylase kn-keyword=L-methionine decarboxylase en-keyword=pyridoxal 5’-phosohate kn-keyword=pyridoxal 5’-phosohate en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=decarboxylation of L-methionine kn-keyword=decarboxylation of L-methionine END start-ver=1.4 cd-journal=joma no-vol=66 cd-vols= no-issue=8 article-no= start-page=1661 end-page=1673 dt-received= dt-revised= dt-accepted= dt-pub-year=1954 dt-pub=19540831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the immunity of Newcastle disease virus Part II: The antigenicity of Newcastle disease virus produced from the hosts' organs infected with it kn-title=Newcastle Disease Virusの免疫に関する研究 第2編 感染動物臓器よりつくつたNewcastle virus vaccineの抗原性に就て en-subtitle= kn-subtitle= en-abstract= kn-abstract=Although there are many investigations about the effects of various antigens of the Newcastle disease virus, the author makes reports on the antigenicity of this virus and also on the immune effects of various fractions obtained on the way of purification of the used materials. The materials chosen for this research were the fowl brains and chick-embryos infected with this virus. 1) In the case where the tissue emulsion was chosen as vaccine, the emulsized tissue as it was had very remarkable effect. 2) Whether formal dehyde or merzonin may be used for inactivation, the immune effect remained to the same degree. 3) The condensation of chorioallantoic fluid to one-fifth of it by Brumfield's methanol sedimentation method, showed great loss of antigenicity, having the hemagglutination and infectious titers of only one-second of those of the original chorioallantoic fluid. 4) The purified and non-purified antigens of the infected chorioallantoic fluid and of 10% emulsions of the fowl brains and chick-embyros were inoculated into the hearts of chickens. Then the purified antigen of chorioallantoic fluid and non-purified antigen of emulsized chick-embryos showed better results than the others, in all of the tests of sen sitized cell agglutination, hemagglutination and of protection against infection. 5) The Newcastle disease virus had its kaolin-adsorption and -dissociation points at pH 4.0 and 8.0 respectively, and the adsorption on kaolin was well performed by repeating it twice. 6) Studying the nitrogen quantity and infectious titer of the kaolin-adsorption and -dissociation products of 10% fowl brain emulsions, the supernatant of adsorption was rich in tissue components and poor in viruses, but that of dissociation was rich in viruses and poor in tissue components. 7) The portion, which is rich in tissue elements and poor in viruses, showed greater antigenicity than the other portion, when they were inactivated and inoculated into chick-hearts. Considering the above-mentioned results, the emulsized chick-embryos as these are seem to be the best one as the antigen of Newcastle disease virus, in view of the intensity of the antigenicity, and the possibility of intramuscular injection and mass production. en-copyright= kn-copyright= en-aut-name=NakagawaFumio en-aut-sei=Nakagawa en-aut-mei=Fumio kn-aut-name=中川文雄 kn-aut-sei=中川 kn-aut-mei=文雄 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部細菌学教室 END start-ver=1.4 cd-journal=joma no-vol=66 cd-vols= no-issue=5 article-no= start-page=919 end-page=932 dt-received= dt-revised= dt-accepted= dt-pub-year=1954 dt-pub=19540531 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The experimental study of Colibacteriophage Report II On the phage growth by nucleus & nuclear substance of blood corpuscle kn-title=大腸菌ファージの実験的研究 第二編 血球核及び核様物に依る大腸菌ファージの増殖について en-subtitle= kn-subtitle= en-abstract= kn-abstract=In my method for the separation and purification of the colibacteriophage, I employed the chromatograph; but I examined the bacteriosolving phenomenon of the phage by using host bacteriofiltrate and rabitt antiserum for host bacterium, as well as, nucleus and nuclear substance of the blood corpuscle of cock and tortoise. In the former I did not get a good result, but I found that the phage growth was accelerated in a high degree in the latter, and consequently this report was written. First, I defibrinierted the 2cc. blood of cook and tortoise, and made the bloodsuspension by ordinary method and then centrifuged it; added distilled water to the blood-corpuscle after the centrifugation, oscillated, mixed well, and demolished the protoplasm by distilled water. As soon as the white nucleus is suspended uniformly, physiological salt, water is added to it in a large quantity; dissolving the pure dregs which were obtained after severaltimes of centrifugation in 5cc. salt water, I presented the same substance that was completely solved for experiment. Then I dyed and microscoped it, and noticed thickly dyeing nucleus as well as a large number of fine granular substance dottes arround it. The strength of colibacteriophage as contrast was 10(-3), but it rose to 10(-27) when the nucleus and nuclear substance of cock was added to it so that it might he diluted same as the first. Moreover, the strength of phage rose to 10(-20) in the filtrate of this nucleus and nuclear substance. In regard to nucleus and nuclear substance of tortoise, the strength of phage was not so strong as cock, but it rose to 10(-14) When I heated the nucleus and nuclear substance of cock bloodcorpuscle in the water bath, the degree of growth facility decreased together with the heat, and it seemed that the effect of growth facility is lostt almost at 100-120°C. "The Feulgen Reaktion" of nucleus and nuclear substance of the blood was all positive, and it increased the degree of transparence together with the heat. It was discovered that. DNA is contained in the nucleus of blood together with a little of RNA, and it is difficult to separate the pure nucleus from the blood, so it is considered apparently that the protoplasm has lost its way within it. In a word, both the nucleus and the nuclear substance of the blood are considered that they play an important part in the growth of coli bacteriophage. en-copyright= kn-copyright= en-aut-name=SasakiK. en-aut-sei=Sasaki en-aut-mei=K. kn-aut-name=佐々木峻 kn-aut-sei=佐々木 kn-aut-mei=峻 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部衛生学教室 END start-ver=1.4 cd-journal=joma no-vol=68 cd-vols= no-issue=11 article-no= start-page=2083 end-page=2092 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=19561130 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the metabolism of Rickettsia kn-title=Rickettsiaの代謝に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The auther tried the partial purification of rickettsiae proliferated in the embryonated egg yolk sacks, and measured the respiration of these partially purified rickettsiae by the usual manometric technique. The results are as follows: 1. By partial purification, it was possible to obtain more purified and not inactivated rickettsiae. 2. As a result of oxygen consumption measurement with Warburg manometer, glutamate, succinate and aspartate were strongly oxidized, and, at the same time, α-ketoglutarate, fumarate, malate, oxaloacetate, pyruvate and β-glycerophosphate were also oxidized pretty well. 3. All enzymic inhibitors showed some inhibition, of which most remarkable was that by monoiodoacetate. 4. The inhibitive action of various antibiotics is varied according to their concentration. However, aureomycin proved to show always very remarkable inhibition. 5. Though incomplete, Rickettsia tsutsugamushi has its proper metabolic system, and is inferred to keep its proliferation by completing its metabolic system in support or connection with living cells en-copyright= kn-copyright= en-aut-name=NakayamaTadao en-aut-sei=Nakayama en-aut-mei=Tadao kn-aut-name=中山忠夫 kn-aut-sei=中山 kn-aut-mei=忠夫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部微生物学教室 END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=7-8 article-no= start-page=687 end-page=698 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=19890831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and characterization of HBs antigen from hepatoma huGK-14 cell line kn-title=ヒト培養肝癌細胞の産生するHBs抗原の精製方法とその物理化学的特性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=HBs antigen was purified from the culture fluid of hepatoma huGK-14 cell line and its physico-chemical properties were studied. The purification consists of following steps: concentration of culture fluid by membrane filtration, affinity column chromatography (anti-HBs monoclonal antibody column and anti-human serum albumin antibody column), and ultracentrifugation (isopycnic centrifugation in CsCl density gradient and rate zonal centrifugation on sucrose gradient). Highly purified (purity>99%) HBs antigen was isolated with an overall yield of about 40%. The HBs antigen showed uniform spherical particles (diameter: 23.2±2.9nm) and had a specific gravity of 1.20g/cm3. The purified HBs antigen yielded, in SDS-PAGE (under reducing conditions), four protein bands with apparent molecular weights of 22,000 and 26,000 (the two major bands), and 44,000 and 47,000. The two proteins of molecular weights of 26,000 and 47,000 are likely to be glycosylated, as these were several fold reduced when the cells were cultured in the presence of Tunicamycin. Amino acid analysis, Edman degradation, carboxypeptidase digestion, and ultraviolet absorption spectrum indicated that the HBs antigen from hepatoma cells is very similar to that derived from human plasma. en-copyright= kn-copyright= en-aut-name=OdaMunehiro en-aut-sei=Oda en-aut-mei=Munehiro kn-aut-name=小田宗宏 kn-aut-sei=小田 kn-aut-mei=宗宏 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源病理学教室 en-keyword=Hepatoma kn-keyword=Hepatoma en-keyword=Cell culture kn-keyword=Cell culture en-keyword=HBs antigen kn-keyword=HBs antigen en-keyword=Purification kn-keyword=Purification en-keyword=Characterization kn-keyword=Characterization END start-ver=1.4 cd-journal=joma no-vol=101 cd-vols= no-issue=5-6 article-no= start-page=659 end-page=672 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Expression and purification of the HIV-1 env gene products in Escherichia coli kn-title=HIV-1エンベロープ蛋白質の大腸菌での発現と精製 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To purify the HIV-1 envelope protein with antigenic reactivity, the Pvu II-Bgl II fragment of the HIV-1 env gene, from the Pvu II site to the second Bgl II site, encoding the carboxyl terminal 180 amino acids of the viral surface protein (SU, gp120) was molecularly cloned in Escherichia coli strain HB101 using protein A expression-shuttle vector pRIT5. The pRIT5 contains the protein A gene, encoding the secretion signal and IgG binding domain of protein A with the upstream promoter and the downstream multicloning sites, as well as the two replication sites for Escherichia coli and Staphylococcus aureus. A fused protein with the molecular weight of about 55 kilodaltons was produced, which showed the same reactivity as the native protein A against rabbit serum IgG on Western blotting analysis. Most of the fused protein in the periplasmic space was degraded, while the complete fused protein inside the cells was recovered as an insoluble protein. The fused protein was solubilized with sodium dodecylsulfate (SDS), partially purified by IgG sepharose affinity chromatography, and completely purified by SDS-polyacrylamide gel electrophoresis. The quantity of the expressed fused protein was estimated about 1% of the total proteins. The purified fused protein contained 516 amino acids with Mr54, 976, consisting of 305 amino acids of the IgG binding domain of protein A, 5 amino acids derived from polylinker, a carboxyl terminal 180 amino acids of the HIV-1 envelope surface protein gp120, and 26 amino acids derived from the pUC19 sequence. en-copyright= kn-copyright= en-aut-name=ZhangBo en-aut-sei=Zhang en-aut-mei=Bo kn-aut-name=張波 kn-aut-sei=張 kn-aut-mei=波 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=HIV-1 kn-keyword=HIV-1 en-keyword=エンベロープ蛋白質 kn-keyword=エンベロープ蛋白質 en-keyword=発現ベクター kn-keyword=発現ベクター en-keyword=Protein A kn-keyword=Protein A en-keyword=IgG-Sepharoseカラム kn-keyword=IgG-Sepharoseカラム END start-ver=1.4 cd-journal=joma no-vol=207 cd-vols= no-issue=4 article-no= start-page=621 end-page=632 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20042 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and cDNA cloning of the ovigerous-hair stripping substance (OHSS) contained in the hatch water of an estuarine crab Sesarma haematocheir en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The egg attachment system of an estuarine crab Sesarma haematocheir is formed on the maternal ovigerous hairs just after egg laying, and slips off these hairs just after hatching. The stripping is caused by an active factor that we call OHSS (ovigerous-hair stripping substance), which is released by the embryo upon hatching. OHSS was purified, and its active form had a molecular mass of 25?kDa. The cDNA of OHSS cloned from an embryonic cDNA library was 1759?bp long, encoding 492 amino acids in a single open reading frame (ORF). The C-terminal part of the predicted protein was composed of a trypsin-like serine protease domain, with homology to counterparts in other animals of 33?38%. The predicted protein (54.7?kDa) secreted as a zymogen may be cleaved post-translationally, separating the Cterminal from the N-terminal region. The OHSS gene was expressed in the embryo at least 2 weeks before hatching. Expression was also detected in the zoea larva 1 day after hatching and in the brain of the female. However, it was not detected in the muscle, hepatopancreas or ovigerous seta of the female. Ultrastructural analysis indicated that the material investing maternal ovigerous hair, i.e. the outermost layer (E1) of the egg case, is attached at the special sites (attachment sites) arranged at intervals of 130?160?nm on the hair. It is suggested that OHSS acts specifically at these sites, lysing the bond with the coat, thus disposing of the embryo attachment system. This enables the female to prepare the next clutch of embryos without ecdysis.

en-copyright= kn-copyright= en-aut-name=GusevOleg en-aut-sei=Gusev en-aut-mei=Oleg kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=IkedaHideki en-aut-sei=Ikeda en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OkochiTetsushi en-aut-sei=Okochi en-aut-mei=Tetsushi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=LeeJae Min en-aut-sei=Lee en-aut-mei=Jae Min kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HatakeyamaMasatsugu en-aut-sei=Hatakeyama en-aut-mei=Masatsugu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KobayashiChiyoko en-aut-sei=Kobayashi en-aut-mei=Chiyoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=AgataKiyokazu en-aut-sei=Agata en-aut-mei=Kiyokazu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=YamadaHidenori en-aut-sei=Yamada en-aut-mei=Hidenori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=SaigusaMasayuki en-aut-sei=Saigusa en-aut-mei=Masayuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=National Institute of Agrobiological Sciences affil-num=5 en-affil= kn-affil=National Institute of Agrobiological Sciences, Owashi affil-num=6 en-affil= kn-affil=RIKEN, Hyougo affil-num=7 en-affil= kn-affil=RIKEN, Hyougo affil-num=8 en-affil= kn-affil=Okayama Univresity affil-num=9 en-affil= kn-affil=Okayama University en-keyword=crab kn-keyword=crab en-keyword=Sesarma (or Chiromantes) haematocheir kn-keyword=Sesarma (or Chiromantes) haematocheir en-keyword=ovigerous hair kn-keyword=ovigerous hair en-keyword=embryo attachment system kn-keyword=embryo attachment system en-keyword=investment coat kn-keyword=investment coat en-keyword=stripping kn-keyword=stripping en-keyword= ovigerous-hair stripping substance (OHSS) kn-keyword= ovigerous-hair stripping substance (OHSS) en-keyword= serine protease. kn-keyword= serine protease. END start-ver=1.4 cd-journal=joma no-vol=64 cd-vols= no-issue=1 article-no= start-page=1 end-page=9 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=201002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Epidemiology of Chlamydophila caviae-like Chlamydia Isolated from Urethra and Uterine Cervix en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In 2000, chlamydial strains OK133 and OK135 were isolated from 2 female patients with cervicitis. These strains were unresponsive to commercially available PCR and LCR test kits for the diagnosis of Chlamydia trachomatis infection, and their phenotypic characteristics were very similar. The OK135 nucleotide sequence in MOMP-VD2 gene closely resembled that of Chlamydophila caviae GPIC. A similar strain was isolated in 2003 from a male patient OKM2 with urethritis, from which the strain SC10-6 was cloned by the plaque purification method. The nucleotide sequence of the entire MOMP gene of SC10-6 was exactly the same as that of OK135. Thus, the strains OK135 and SC10-6, together with OK133, have been called C. caviae-like Chlamydia. We designed primers for nested PCR assay, the product of which showed a single-band 311-bp fragment, to detect C. caviae-like Chlamydia. Of swab specimens obtained from 202 patients from 2003 to 2006 (119 male and 83 female patients), 18 specimens (8.9%) from 14 male and 4 female patients were positive, suggesting that C. caviae-like Chlamydia infection is rather common. Thus far, it has not been determined whether C. caviae-like Chlamydia is pathogenic for humans.

en-copyright= kn-copyright= en-aut-name=MuraoWataru en-aut-sei=Murao en-aut-mei=Wataru kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=WadaKoichiro en-aut-sei=Wada en-aut-mei=Koichiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MatsumotoAkira en-aut-sei=Matsumoto en-aut-mei=Akira kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=FujiwaraMichihisa en-aut-sei=Fujiwara en-aut-mei=Michihisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=FukushiHideto en-aut-sei=Fukushi en-aut-mei=Hideto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=KishimotoToshio en-aut-sei=Kishimoto en-aut-mei=Toshio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=MondenKoichi en-aut-sei=Monden en-aut-mei=Koichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=KariyamaReiko en-aut-sei=Kariyama en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=KumonHiromi en-aut-sei=Kumon en-aut-mei=Hiromi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=2 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=3 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=4 en-affil= kn-affil=Department of Obstetrics and Gynecology, Kawasaki Hospital affiliated with Kawasaki Medical School affil-num=5 en-affil= kn-affil=Department of Applied Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University affil-num=6 en-affil= kn-affil=Department of Virology I, The National Institute of Infectious Diseases affil-num=7 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=8 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences affil-num=9 en-affil= kn-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences en-keyword=Chlamydophila caviae-like Chlamydia kn-keyword=Chlamydophila caviae-like Chlamydia en-keyword=urethra kn-keyword=urethra en-keyword=uterine cervix kn-keyword=uterine cervix en-keyword=epidemiology kn-keyword=epidemiology en-keyword=sexually transmitted infection kn-keyword=sexually transmitted infection END start-ver=1.4 cd-journal=joma no-vol=24 cd-vols= no-issue=1 article-no= start-page=49 end-page=64 dt-received= dt-revised= dt-accepted= dt-pub-year=1970 dt-pub=197002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Activation and isolation of mitochondrial adenosine triphosphatase by ultrasonic irradiation en-subtitle= kn-subtitle= en-abstract= kn-abstract=

With the purpose to clarified the mode of localization and mechanisms of activation of ATPase in the mitochondrial membrane, analyses were made on the properties of mitochondrial ATPase from the structural and functional aspects. The activation of ATPase by DNP and Mg++ and the oligomycin sensitivity were investigated in a series of inner membrane fragment samples obtained by ultrasonic irradiation and those samples obtained in the processes of isolation and purification of ATPase from rat liver mitochondria and beef heart mitochondria in parallel with electron microscope observations. As a result it has been found that the membrane fragments obtained from rat liver and beef heart mitochondria by ultrasonication exhibited high respiratory activity and unmasked ATPase activity which was charac? terized by remarkable stimulation by Mg++ and inhibition by oligomycin and azide. Therefore, mitochondrial ATPase seems to be bound fairly closely to the inner mitochondrial membrane. In the membrane fragments prepared by ultrasonication of intact mitochondria, ATPase activity was stimulated by DNP, but in the supernatant fractions was not. On the other hand, the supernatant fraction obtained from BHM and inner membrane fragments by severe sonication exhibits a marked ATPase activity and the activity incresed in each step of the purification on the treatments with acid, protamine and heat. Especially in the case of membrane fragments the protamine treatment can be omitted. Electron microscope observation of the fractions in each step of the purification proved the head pieces to be ATPase. The ATPase activity of solubilized head pieces is insensitive to oligo. mycin and coincides with the soluble ATPase of PULLMAN etat. (8) in the points of its cold labile property and optimum pH, but it shown no accele. ration of ATPase activity by DNP.

en-copyright= kn-copyright= en-aut-name=YamamotoGoki en-aut-sei=Yamamoto en-aut-mei=Goki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TsukamotoHiromichi en-aut-sei=Tsukamoto en-aut-mei=Hiromichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=28 cd-vols= no-issue=1 article-no= start-page=1 end-page=6 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=197402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on the cornin extracted from bovine liver. I. Purification of the cornin and its physico-chemical properties en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A factor, cornin, inhibiting the growth of L cells cultured in monolayer was extracted from bovine liver with boiling water and was partially purified by gel filtration with Sephadex G-200. The factor was (1) precipitable with ethanol at the concentration between 70% and 90%, (2) impermeable through dializing memo brane, (3) eluted as the last peak at the gel filtration and (4) containing protein and RNA but no DNA.

en-copyright= kn-copyright= en-aut-name=OhtsukiHisashi en-aut-sei=Ohtsuki en-aut-mei=Hisashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=22 cd-vols= no-issue=4 article-no= start-page=175 end-page=184 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=196808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification of the head-pieces of the elementary particles from beef heart mitochondria: their morphological structure and enzymatic activity en-subtitle= kn-subtitle= en-abstract= kn-abstract=

1. In order to obtain direct evidence for the enzymatic identification of the head-pieces of the elementary particles in the inner mitochondrial membrane, the head-pieces were detached by sonication from the isolated inner membrane of beef heart mitochondria, purified by pursuing the particles with the electron microscope, and analyzed for enzymatic properties. 2. Electron microscope examination revealed that the isolated headpieces are the spherical particles about 90À in diameter which are quite similar in appearance to the head-pieces of the elementary particles lining the inner mitochondrial membranes. 3. The head-pieces are identified as ATPase sensitive to oligomysin when attached by stalks to the membrane, and become insensitive when detached or purified from the membrane. 4. The head-piece is labile to cold with respect to ATPase activity and morphology.

en-copyright= kn-copyright= en-aut-name=KoshibaK. en-aut-sei=Koshiba en-aut-mei=K. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=YamamotoG. en-aut-sei=Yamamoto en-aut-mei=G. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=InoharaR. en-aut-sei=Inohara en-aut-mei=R. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OdaT. en-aut-sei=Oda en-aut-mei=T. kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=23 cd-vols= no-issue=5 article-no= start-page=357 end-page=376 dt-received= dt-revised= dt-accepted= dt-pub-year=1969 dt-pub=196910 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Molecular basis of structure and function of the microvillus membrane of intestinal epithelial cells en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Correlation of molecular structure with biochemical functions of the plasma membrane of the microvilli of intestinal epithelial cells has been investigated by biochemical and electron microscopic procedures. Repeating particles, measuring approximately 60 Åin diameter, were found on the surface of the microvilli membrane which had been isolated or purified from rabbit intestinal epithelial cells and negatively stained with phosphotungstic acid. These particles were proved to be inherent components of the microvillus membrane, attached to the outer surface of its trilaminar structure, and were designated as the elementary particles of the microvilli of intestinal epithelial cells. Biochemical and electron microscopic identification of these elementary particles has been carried out by isolation of the elementary particles with papain from the isolated microvillus membrane, followed by purification of the particles by chromatographies on DEAE-cellulose and Sephadex columns. The partially purified particles containing invertase and leucine aminopeptidase are similar in size and structure to those of the elementary particles in the microvillus membrane. Evidence indicates that each of the elementary particles coincide with or include an enzyme molecule such as disaccharidase or peptidase, which carry out the terminal hydrolytic digestion of carbohydrates and proteins, respectively, on the surface of the microvillus membrane. Magnesium ionactivated adenosine triphosphatase and alkaline phosphatase cannot be solubilized with papain but remains in the smooth-surface membrane after the elementary particles have been removed. Cytochemical electron microscopic observation revealed that the active site of magnesium ion-activated adenosine triphosphatase is localized predominantly in the inner surface of the trilaminar structure of the microvillus membrane.

en-copyright= kn-copyright= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SekiShuji en-aut-sei=Seki en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSekiko en-aut-sei=Watanabe en-aut-mei=Sekiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=21 cd-vols= no-issue=2 article-no= start-page=79 end-page=89 dt-received= dt-revised= dt-accepted= dt-pub-year=1967 dt-pub=196704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and fine structure of reduced coenzyme Q-cytochrome C reductase in the mitochondrial membrane en-subtitle= kn-subtitle= en-abstract= kn-abstract=

For the purpose of revealing the molecular organization of the mitochondrial membrane the authors attempted to clarify the fine structure of reduced coenzyme Q-cytochrome c reductase and also studied how the CoQH2-cyt. c reductase is arranged in the mitochondrial membrane by systematic analyses of fractions from the purification process of CoQH2-cyt. c reductase. 1. Purified CoQH2-cyt. c reductase contained high concentration of cyt. b (9.5 mμmoles per mg protein) and cyt. Cl (4.5 mμmoles per mg protein), and was almost free from cyt. c, a, flavoproteins, primary dehydrogenases and ATPase. The enzyme complex also showed a high specific activity (48 μmoles of cyt. c reduced per mg protein per min at 30°). 2. CoQH2-cyt. c reductase was composed of particles of about 120 Å in diameter with irregular form, some time exhibiting electron opaque cores. In the loose aggregates of the particles, the size of each particle was about 95 Å in diameter. 3. An intimate correlation was observed between the particles of CoQH2cyt. c reductase and those on the surface of the NADH-cyt. c reductase fraction. 4. Regular arrays of uniform particles (about 82 Å in diameter with a center to center distance of about 100 Å) were observed on the surface of the submitochondrial membrane (brown membrane) obtained from beef heart mitochondria by treatment with deoxycholate (0.1 mg / mg protein) and KCl (72 g/l). The correlation between these particles and CoQH2-cyt. c reductase was discussed.

en-copyright= kn-copyright= en-aut-name=SekiShuji en-aut-sei=Seki en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=54 cd-vols= no-issue=2 article-no= start-page=57 end-page=65 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200004 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification, identification and phosphorylation of annexin I from rat liver mitochondria. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Annexin was purified from rat liver mitochondria to an apparent homogeneity with a molecular weight of 35 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The purified mitochondrial annexin (AXmito) was identified as annexin I by an immunoblot analysis using anti-annexin I antibody. The inhibitory effect of AXmito I on porcine pancreatic phospholipase A2 activity was as potent as that of bovine lung annexin I. The presence of annexin I in mitochondria was confirmed by an electron-microscopic study. AXmito I was shown to be phosphorylated by intrinsic protein tyrosine kinases on its tyrosine residues. This annexin was also phosphorylated by protein kinase C.

en-copyright= kn-copyright= en-aut-name=YoshiiKenji en-aut-sei=Yoshii en-aut-mei=Kenji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=SugimotoKatsuyoshi en-aut-sei=Sugimoto en-aut-mei=Katsuyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TaiYuji en-aut-sei=Tai en-aut-mei=Yuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KonishiRyoji en-aut-sei=Konishi en-aut-mei=Ryoji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Kagawa Medical University affil-num=2 en-affil= kn-affil=Kagawa Medical University affil-num=3 en-affil= kn-affil=Kagawa Medical University affil-num=4 en-affil= kn-affil=Kagawa Medical University affil-num=5 en-affil= kn-affil=Kagawa Medical University en-keyword=annexin kn-keyword=annexin en-keyword=mitochondria kn-keyword=mitochondria en-keyword=protein tyrosine kinases kn-keyword=protein tyrosine kinases en-keyword=protein kinase C kn-keyword=protein kinase C en-keyword=Phospholipase A2 kn-keyword=Phospholipase A2 END start-ver=1.4 cd-journal=joma no-vol=33 cd-vols= no-issue=3 article-no= start-page=167 end-page=176 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197906 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Partial purification and biological activities and properties of chick growth factors. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.

en-copyright= kn-copyright= en-aut-name=TakahashiFumio en-aut-sei=Takahashi en-aut-mei=Fumio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=KuramitsuMakoto en-aut-sei=Kuramitsu en-aut-mei=Makoto kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=MatsuiHideki en-aut-sei=Matsui en-aut-mei=Hideki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=ItanoToshifumi en-aut-sei=Itano en-aut-mei=Toshifumi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=MurakamiTetsu-Hide en-aut-sei=Murakami en-aut-mei=Tetsu-Hide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=NishidaIsamu en-aut-sei=Nishida en-aut-mei=Isamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University en-keyword=chick growth factors kn-keyword=chick growth factors en-keyword=cell proliferation kn-keyword=cell proliferation en-keyword=growth regulation kn-keyword=growth regulation en-keyword=DNA and RNA synthesis kn-keyword=DNA and RNA synthesis en-keyword=protein synthesis kn-keyword=protein synthesis END start-ver=1.4 cd-journal=joma no-vol=33 cd-vols= no-issue=2 article-no= start-page=103 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Partial purification and properties of bovine heart catalase. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Catalase was partially purified (about 380-fold purification) from the post-mitochondrial supernatant of bovine heart and compared with catalases from bovine erythrocytes and bovine liver. The electrophoretic mobility in polyacrylamide gel (pH 8.0) of heart catalase was the same as that of erythrocyte catalase and was smaller than that of the liver enzyme. The heart catalase was indistinguishable from erythrocyte catalase in regard to the molecular weights of subunit polypeptides, the inhibition patterns produced by several catalase inhibitors, and specific activity. The pH-activity curve of heart catalase consisted of a characteristic biphasic pattern with a peak at pH 7.5 and a shoulder at pH 10.

en-copyright= kn-copyright= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HataseOsamu en-aut-sei=Hatase en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University en-keyword=catalse kn-keyword=catalse en-keyword=muscle kn-keyword=muscle en-keyword=bovine heart kn-keyword=bovine heart END start-ver=1.4 cd-journal=joma no-vol=33 cd-vols= no-issue=4 article-no= start-page=259 end-page=267 dt-received= dt-revised= dt-accepted= dt-pub-year=1979 dt-pub=197908 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification of antilymphocyte antibody (ALA) from patients with systemic lupus erythematosus (SLE)-immunoabsorption and elution. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Purification of antilymphocyte antibody (ALA) from patients with systemic lupus erythematosus (SLE) was achieved by immunoabsorption and elution. Human tonsil cells or thymocytes were used as absorbents. Complement dependent microcytotoxicity tests showed that, in comparison to the parent sera, the eluate from tonsil cells was eight times, and that from thymocytes four times, more active. Antinuclear activity was eliminated by elution. The ALA was almost entirely IgM, IgG being involved in only a few cases. IgA lacked cytotoxic activity. ALA was directed at both T- and B-cell surface determinants, which suggests that, in SLE, it has a heterogeneous biological composition.

en-copyright= kn-copyright= en-aut-name=YamanaSeizo en-aut-sei=Yamana en-aut-mei=Seizo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=NakamuraZenichi en-aut-sei=Nakamura en-aut-mei=Zenichi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SaitoYoshihito en-aut-sei=Saito en-aut-mei=Yoshihito kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YamamotoMichinori en-aut-sei=Yamamoto en-aut-mei=Michinori kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OfujiTadashi en-aut-sei=Ofuji en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=antilymphocyte antibody kn-keyword=antilymphocyte antibody en-keyword=systemic lupus erythematosus kn-keyword=systemic lupus erythematosus en-keyword=purification kn-keyword=purification END start-ver=1.4 cd-journal=joma no-vol=56 cd-vols= no-issue=2 article-no= start-page=99 end-page=105 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=200204 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Anti-asialoglycoprotein receptor autoantibodies, detected by a capture-immunoassay, are associated with autoimmune liver diseases. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

In autoimmune chronic active hepatitis (AIH) and primary biliary cirrhosis (PBC), various autoantibodies including anti-asialoglycoprotein receptor (ASGPR) antibodies have been found in patients' sera. We have previously developed a mouse monoclonal antibody against rat and human ASGPR. In this study, we developed a capture enzyme-linked immunosorbent assay (ELISA) for detection of anti-ASGPR antibodies using this monoclonal antibody and investigated the occurrence of anti-ASGPR antibodies in the sera of patients with various liver diseases. Serum samples were obtained from 123 patients with various liver diseases, including 21 patients with AIH and 40 patients with PBC. In this capture ELISA, the target antigen in the crude rat liver membrane extracts was captured on the ELISA wells by the ASGPR-specific mouse monoclonal antibody. Thus, the cumbersome process of antigen purification was rendered unnecessary. Using this capture ELISA, we detected the anti-ASGPR antibody in 67% of the patients with AIH, in 100% of the patients with PBC, and in 57% of the patients with acute hepatitis type A. However, the anti-ASGPR antibody was rarely detected in patients with other liver diseases such as primary sclerosing cholangitis and obstructive jaundice. Our findings suggest that this capture ELISA would be useful for the detection of anti-ASGPR antibodies in autoimmune liver diseases.

en-copyright= kn-copyright= en-aut-name=YoshiokaMasao en-aut-sei=Yoshioka en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MizunoMotowo en-aut-sei=Mizuno en-aut-mei=Motowo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=MorisueYoshiko en-aut-sei=Morisue en-aut-mei=Yoshiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=ShimadaMorizou en-aut-sei=Shimada en-aut-mei=Morizou kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=HiraiMichio en-aut-sei=Hirai en-aut-mei=Michio kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=NasuJunichirou en-aut-sei=Nasu en-aut-mei=Junichirou kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OkadaHiroyuki en-aut-sei=Okada en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= en-aut-name=SakaguchiKousaku en-aut-sei=Sakaguchi en-aut-mei=Kousaku kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=8 ORCID= en-aut-name=YamamotoKazuhide en-aut-sei=Yamamoto en-aut-mei=Kazuhide kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=9 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=10 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University affil-num=8 en-affil= kn-affil=Okayama University affil-num=9 en-affil= kn-affil=Okayama University affil-num=10 en-affil= kn-affil=Okayama University en-keyword=autoimmue hepatitis kn-keyword=autoimmue hepatitis en-keyword=primary biliary cirrhosis kn-keyword=primary biliary cirrhosis en-keyword=asialoglycoprotein receptor kn-keyword=asialoglycoprotein receptor en-keyword=autoantibodies kn-keyword=autoantibodies END start-ver=1.4 cd-journal=joma no-vol=35 cd-vols= no-issue=6 article-no= start-page=427 end-page=430 dt-received= dt-revised= dt-accepted= dt-pub-year=1981 dt-pub=198112 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A simple method for purification of rat alpha-fetoprotein by affi-gel blue chromatography and disc electrophoresis en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Alpha-getoprotein (AFP) was purified from fetal rat serum by a combined technique of affinity chromatography with Affi-Gel Blue and disc electrophoresis followed by extraction of AFP from the gel. The purified AFP was immunologically identical with the original AFP in fetal rat serum.

en-copyright= kn-copyright= en-aut-name=MiyazakiMasahiro en-aut-sei=Miyazaki en-aut-mei=Masahiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MatsuuraKazuhiko en-aut-sei=Matsuura en-aut-mei=Kazuhiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WahidSyarifuddin en-aut-sei=Wahid en-aut-mei=Syarifuddin kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=IzumiMasaki en-aut-sei=Izumi en-aut-mei=Masaki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=SatoJiro en-aut-sei=Sato en-aut-mei=Jiro kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Hasanuddin University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Health Research Center, Kagawa University affil-num=6 en-affil= kn-affil=Okayama University en-keyword=purification of rat AFP kn-keyword=purification of rat AFP en-keyword=fetal rat serum kn-keyword=fetal rat serum en-keyword=affinity chromatography kn-keyword=affinity chromatography en-keyword= disc electrophoresis. kn-keyword= disc electrophoresis. END start-ver=1.4 cd-journal=joma no-vol=48 cd-vols= no-issue=2 article-no= start-page=67 end-page=72 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199404 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Partial Purification and Chraracterization of Dendritic Cell Differentiation Factor en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Previously, we reported that interleukin-2 (IL-2)-stimulated helper T cells produced an unknown soluble factor which induced dendritic cell-like differentiation in primary cultures of monocytic leukemia cells and we referred to this factor as dendritic cell differentiation factor (DCDF). In this study, we attempted to purify and characterize DCDF and investigated its biological effect on normal human monocytes. Gel filtration chromatography indicated that the molecular weight of DCDF is approximately 30-35 kDa. Chromatofocusing indicated that the isoelectric point of DCDF is approximately 5.0. DCDF, partially purified by subsequent gel filtration, chromatofocusing, and hydrophobic chromatography, significantly enhanced the HLA-DR expression of normal human monocytes and a human monocytic leukemia cell line, THP-1. This biological activity was not neutralized by any known antibodies to human cytokines. DCDF significantly amplified the T-cell stimulatory activity of monocytes in the allogeneic mixed leukocyte reaction (MLR). Moreover, DCDF significantly enhanced IL-1 beta and IL-6 production by monocytes in a dose-dependent manner. These results suggest that DCDF is a novel human cytokine which stimulates the accessory cell function of monocytes.

en-copyright= kn-copyright= en-aut-name=MiyataniKatsuya en-aut-sei=Miyatani en-aut-mei=Katsuya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TakahashiKiyoshi en-aut-sei=Takahashi en-aut-mei=Kiyoshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=YanaiHiroyuki en-aut-sei=Yanai en-aut-mei=Hiroyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=YoshinoTadashi en-aut-sei=Yoshino en-aut-mei=Tadashi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=AkagiTadaatsu en-aut-sei=Akagi en-aut-mei=Tadaatsu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=dendritic cell kn-keyword=dendritic cell en-keyword=differentiation kn-keyword=differentiation en-keyword=protein purification kn-keyword=protein purification en-keyword=cytokine kn-keyword=cytokine END start-ver=1.4 cd-journal=joma no-vol=32 cd-vols= no-issue=6 article-no= start-page=379 end-page=385 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197812 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A simple large-scale method for separating closed circular form DNA by gel electrophoresis en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The covalently closed form of circular duplex SV40 DNA was separated from the open and linear form of SV40 DNA by agarose gel electrophoresis in a large-scale gel system. The closed circular DNA was recovered from agarose gels by re-electrophoresing the gel slices. The recovery of DNA was about 70%. Electron microscopic analysis showed that the recovered DNA did not have doube- or single-stranded breaks. The recovered DNA can be used without further purification for electron microscopy, as a substrate for experiments using restriction endonuclease and as a template for in vitro RNA synthesis.

en-copyright= kn-copyright= en-aut-name=TanabeNaoko en-aut-sei=Tanabe en-aut-mei=Naoko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HidakaHideyuki en-aut-sei=Hidaka en-aut-mei=Hideyuki kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=WatanabeSekiko en-aut-sei=Watanabe en-aut-mei=Sekiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University en-keyword=separating method kn-keyword=separating method en-keyword=simian virus 4D DNA kn-keyword=simian virus 4D DNA en-keyword=closed circular DNA kn-keyword=closed circular DNA en-keyword=gel electrophoresis kn-keyword=gel electrophoresis END start-ver=1.4 cd-journal=joma no-vol=32 cd-vols= no-issue=4 article-no= start-page=265 end-page=272 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Respiratory syncytial virus. I. Concentration and purification of the infectious virus en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Respiratory syncytial (RS) virus can be purified without losing its infectivity provided that each step of purification is carried out using NT buffer containing over 20% sucrose. Firstly, the virus grown on HES cells is efficiently removed from the culture fluid by precipitating with polyethylene glycol (PEG) 6,000, and the precipitate is suspended in a small amount of 20% sucrose-NT buffer, which results in about a 24-fold concentration of the original material. Then this suspension is centrifugated through 30% sucrose-NT buffer to obtain pellets, which are again suspended in 20% sucrose-NT buffer. This suspension is further centrifuged by discontinuous and linear sucrose density gradient. Finally, the specific infectivity of the purified virus was increased about 3,000-fold over that of the original material.

en-copyright= kn-copyright= en-aut-name=UebaOsamu en-aut-sei=Ueba en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=respiratory syncytial virus kn-keyword=respiratory syncytial virus en-keyword=purification kn-keyword=purification END start-ver=1.4 cd-journal=joma no-vol=29 cd-vols= no-issue=5 article-no= start-page=355 end-page=366 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=197510 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and immunochemical characterization of alpha-fetoprotein from rat fetal serum and liver en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Two alpha1-globulin bands of fetal serum with relative mobilities against bromophenol blue of 0.55 and 0.58 on 7% polyacrylamide gel electrophoresis reacted with a monospecific rabbit antiserum to alpha-fetoprotein (AFP). The former globulin band was clearly detected in the fetal liver supernatant. AFP was immunochemically purified from both the fetal serum and liver, and their electrophoretic and immunochemical properties were compared. Liver AFP purified by immunoadsorbent column yielded electrophoretic mobilities and relative amounts of the two electrophoretically distinct components identical with the purified serum AFP. The immunological reactivity of the two components of the purified preparations from serum and liver against the monospecific anti-AFP serum was also indistinguishable. After the removal of the sialic acid residues from purified serum and liver AFP by treatment with neuraminidase for 6 to 12 hr, disc electrophoretic patterns on 5% polyacrylamide gel and immunoelectrophoretic patterns of the treated AFP were found to be closely similar in both preparations. It may be possible to conclude that serum and liver AFP are structurally indistinguishable and probably identical.

en-copyright= kn-copyright= en-aut-name=WatanabeAkiharu en-aut-sei=Watanabe en-aut-mei=Akiharu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=MoriOsamu en-aut-sei=Mori en-aut-mei=Osamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=TakedaKazuhisa en-aut-sei=Takeda en-aut-mei=Kazuhisa kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=KosakaKiyowo en-aut-sei=Kosaka en-aut-mei=Kiyowo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=43 cd-vols= no-issue=2 article-no= start-page=127 end-page=129 dt-received= dt-revised= dt-accepted= dt-pub-year=1989 dt-pub=198904 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Rapid purification of squirrel monkey retrovirus-H major gag protein by high performance liquid chromatography. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

The major gag protein (p34) of squirrel monkey retrovirus-H was purified in one chromatographic step by anion-exchange high performance liquid chromatography. The virus in a crude fraction was disrupted with Brij 35 in the presence of three kinds of protease inhibitors. The soluble virus lysate was injected into a Polyanion SI column, and p34 was eluted with a linear salt gradient. The recovery of the protein was about 60%. The purified p34 was nearly homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining.

en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=HatsushikaMasao en-aut-sei=Hatsushika en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeSekiko en-aut-sei=Watanabe en-aut-mei=Sekiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University en-keyword=retrovirus kn-keyword=retrovirus en-keyword=gag protein kn-keyword=gag protein en-keyword=protein purification kn-keyword=protein purification en-keyword=high performance liquid chromatography kn-keyword=high performance liquid chromatography END start-ver=1.4 cd-journal=joma no-vol=31 cd-vols= no-issue=3 article-no= start-page=203 end-page=209 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=197706 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and some characteristics of liver cytosol cornin, an antimitotic substance from rat liver cytosol en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Further purification and characterization are reported on rat cytosol cornin (RLCC), an antimitotic substance. Fraction I (purified RLCC) was purified more than 10-fold from crude RLCC with Sephadex G-50 column chromatography and showed a remarkable inhibitory effect on division of inseminated sea urchin eggs and mouse fibroblast cells. Fraction I was observed as one spot, and the molecular weight was estimated to be about 25,000 by thin layer gel filtration. Fraction I contained protein (92%) and RNA (8%), but the antimitotic activity was scarcely affected by treatment by pancreatic RNase. The protein of Fraction I was separated into two bands by SDS-polyacrylamide gel electrophoresis, and the molecular weight was estimated as 10,000 and 15,000, respectively. The 50% inhibition dose of Fraction I on the first division of inseminated sea urchin eggs and on proliferation of mouse L cells was about 2.5 X 10(-5) g/ml and 5 X 10(-4) g/ml, respectively. The yield of fraction I was about 35 mg from 100 g rat liver.

en-copyright= kn-copyright= en-aut-name=InabaKozo en-aut-sei=Inaba en-aut-mei=Kozo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=DoiAkitaka en-aut-sei=Doi en-aut-mei=Akitaka kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=NisidaIsamu en-aut-sei=Nisida en-aut-mei=Isamu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=51 cd-vols= no-issue=2 article-no= start-page=55 end-page=62 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=199704 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=A 55-kDa endonuclease of mammalian mitochondria: comparison of its subcellular localization and endonucleolytic properties with those of endonuclease G en-subtitle= kn-subtitle= en-abstract= kn-abstract=

A novel endonuclease of 55-kDa was found in rat liver mitochondria by a zymographic assay, in addition to the 29 kDa enzyme that is well-known as endonuclease G (Endo G). Subcellular localization of these enzymes in rat liver cells was examined by biochemical fractionation. Endo G was located in both nuclei and mitochondria as has been previously reported, while the 55-kDa enzyme was only detected in the mitochondrial fraction. The levels of the endonucleases in the mitochondria varied greatly among the rat organs, and the activity in the heart was about 30 times higher than that in the liver. The 55-kDa enzyme and Endo G were extracted from bovine heart mitochondria with 0.4 M NaCl. During purification the 55-kDa enzyme and Endo G were copurified because of their similar chromatographic behavior, so they were separated by gel filtration or electrophoresis in the presence of SDS and the proteins were then renatured. The nucleolytic properties of the 55-kDa enzyme resembled those of Endo G and other known mitochondrial nucleases. The enzyme degraded single-stranded DNA more rapidly than duplex DNA at a weak alkaline pH, requiring Mg2+ or Mn2+ but not Ca2+ or Zn2+. Nicks generated by the enzyme had 5′-P and 3′-OH ends. The 55-kDa enzyme, like Endo G, displayed an unusually strong preference to nick within a (dG)n ? (dC)n tract.

en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HasegawaHaruko en-aut-sei=Hasegawa en-aut-mei=Haruko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=KaminakaShinobu en-aut-sei=Kaminaka en-aut-mei=Shinobu kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University of Science affil-num=2 en-affil= kn-affil=Okayama University of Science affil-num=3 en-affil= kn-affil=Okayama University of Science en-keyword=activity gel analysis kn-keyword=activity gel analysis en-keyword=endonuclease kn-keyword=endonuclease en-keyword=endonuclease G kn-keyword=endonuclease G en-keyword=mitochondrial DNA kn-keyword=mitochondrial DNA en-keyword=oxidative damage kn-keyword=oxidative damage END start-ver=1.4 cd-journal=joma no-vol=36 cd-vols= no-issue=3 article-no= start-page=187 end-page=197 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=198206 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and characterization of cysteine aminotransferase from rat liver cytosol. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Cysteine aminotransferase (L-cysteine: 2-oxoglutarate aminotransferase, EC 2.6.1.3) was purified over 400-fold from the high-speed supernatant fraction of rat liver. The purified enzyme was homogeneous as judged by gel filtration, isoelectric focusing and disc electrophoresis. The molecular weight of the enzyme was about 74,000 by gel filtration and the isoelectric point was 6.2 (4 degrees C). The enzyme catalyzed transamination between L-cysteine and 2-oxoglutarate and the reverse reaction. The optimum pH was 9.7. The Km value for L-cysteine was 22.2 mM, and that for 2-oxoglutaric acid was 0.06 mM. L-Aspartate was a potent inhibitor of the cysteine aminotransferase reaction. The enzyme was very active toward L-alanine 3-sulfinic acid at pH 8.0, and was also very active toward L-aspartic acid (Km = 1.6 mM). Ratios of activities for L-aspartic acid and L-cysteine were essentially constant during the purification of the enzyme. Evidence based on substrate specificity, enzyme inhibition, and physicochemical properties indicates that cytosolic cysteine aminotransferase is identical with cytosolic aspartate aminotransferase (L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1).

en-copyright= kn-copyright= en-aut-name=AkagiReiko en-aut-sei=Akagi en-aut-mei=Reiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University en-keyword=cysteine aminotransferase kn-keyword=cysteine aminotransferase en-keyword=enzyme purification kn-keyword=enzyme purification en-keyword=aspartate aminotransferase kn-keyword=aspartate aminotransferase END start-ver=1.4 cd-journal=joma no-vol=50 cd-vols= no-issue=3 article-no= start-page=131 end-page=137 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=199606 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Purification and characterization of a 39kDa apurinic/apyrimidinic endonuclease from mouse ascites sarcoma cells. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

<P>We purified an apurinic/apyrimidinic (AP) endonuclease from mouse ascites sarcoma (SR-C3H/He) cells. The enzyme showed nicking activity on acid-depurinated DNA but not on untreated, intact DNA. It also showed priming activity for DNA polymerase on both acid-depurinated and bleomycin-damaged DNA. The priming activity on bleomycin-damaged DNA was two times higher than that on an acid-depurinated DNA. The enzymatic properties indicate that the enzyme is a class II AP endonuclease having DNA 3' repair diesterase activity. The purified enzyme has a molecular weight of 39,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The optimal pH for AP endonuclease activity was 8.0 in 50 mM Tris-HCl buffer. The AP endonuclease activity depended on divalent cation such as Mg2+ and Co2+ ions, and was inhibited by 2 mM EDTA with no addition of the divalent cation. An appropriate concentration of sodium or potassium salt stimulated the activity. Partial digestion of the AP endonuclease with Staphylococcus aureus V8 protease produced 4 major peptide fragments which may be used for protein sequencing.</P>

en-copyright= kn-copyright= en-aut-name=WakabayashiHajime en-aut-sei=Wakabayashi en-aut-mei=Hajime kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=TsujiTakao en-aut-sei=Tsuji en-aut-mei=Takao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=SekiShuji en-aut-sei=Seki en-aut-mei=Shuji kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama Univeristy en-keyword=AP endonuclease kn-keyword=AP endonuclease en-keyword=DNA 3' repair diesterase kn-keyword=DNA 3' repair diesterase en-keyword=DNA repair enzyme kn-keyword=DNA repair enzyme en-keyword=mouse ascites sarcoma cells kn-keyword=mouse ascites sarcoma cells END start-ver=1.4 cd-journal=joma no-vol=38 cd-vols= no-issue=4 article-no= start-page=341 end-page=347 dt-received= dt-revised= dt-accepted= dt-pub-year=1984 dt-pub=198408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Partial purification of Simian virus 40 large T antigen by immunoaffinity chromatography and its detection by enzyme-linked immunosorbent assay. en-subtitle= kn-subtitle= en-abstract= kn-abstract=

Simian virus 40 (SV40) large T antigen was partially purified from small amounts of SV40-infected and SV40-transformed cells by immunoaffinity chromatography with high recovery. T antigen, in both crude and partially purified states, was detected rapidly by a sensitive and quantitative enzyme-linked immunosorbent assay (ELISA). Stability of the partially purified T antigen was found to increase by addition of 0.01% bovine serum albumin (BSA).

en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= en-aut-name=HatsushikaMasao en-aut-sei=Hatsushika en-aut-mei=Masao kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=2 ORCID= en-aut-name=ShigeharaTsuguya en-aut-sei=Shigehara en-aut-mei=Tsuguya kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=3 ORCID= en-aut-name=WatanabeSekiko en-aut-sei=Watanabe en-aut-mei=Sekiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=4 ORCID= en-aut-name=OmuraSachiko en-aut-sei=Omura en-aut-mei=Sachiko kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=5 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=6 ORCID= en-aut-name=OdaTakuzo en-aut-sei=Oda en-aut-mei=Takuzo kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=Okayama University affil-num=2 en-affil= kn-affil=Okayama University affil-num=3 en-affil= kn-affil=Okayama University affil-num=4 en-affil= kn-affil=Okayama University affil-num=5 en-affil= kn-affil=Okayama University affil-num=6 en-affil= kn-affil=Okayama University affil-num=7 en-affil= kn-affil=Okayama University en-keyword=SV40 T antigen kn-keyword=SV40 T antigen en-keyword=affinity chromatography kn-keyword=affinity chromatography en-keyword=ELISA kn-keyword=ELISA END start-ver=1.4 cd-journal=joma no-vol=10 cd-vols= no-issue=1 article-no= start-page=47 end-page=55 dt-received= dt-revised= dt-accepted= dt-pub-year=1956 dt-pub=195601 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Studies on Bile Pigments III. Biliverdins Derived From Natural Bilirubins en-subtitle= kn-subtitle= en-abstract= kn-abstract=

1. Absorption maxima of hydrochloric biliverdins derived from the natural indirect bilirubin existed at 680 mμ and 375 mμ, but the maxima of biliverdins purified on the column of silica gel existed at 640 mμ and 390 mμ. 2. The natural salt-form bilirubin was oxidized by hydrochloric acid to biliverdin, of which absorption maxima existed at 685 mμ and 370 mμ in a methanolic solution as well as in 5% hydrochloric methanol, but the purified biliverdin in chloroform solution showed the maxima at 640 mμ and 390 mμ. 3. The natural ester-form bilirubin could be transformed into biliverdin by oxidation of its alcoholic solution in the presence of hydrochloric acid. The crude biliverdin had absorption maxima at 645 to 655 mμ, 600 mμ and 320 mμ, and the crude hydrochloric biliverdin had the maxima at 665 to 675 mμ, 620 mμ and in the near ultra-violet range, while the purified biliverdin in chloroform solution had the maxima at 640 mμ and 380 mμ. 4. The biliverdins derived from the indirect, salt-form and ester-form bilirubin had quite similar absorption maxima after purifications by adsorption chromatography.

en-copyright= kn-copyright= en-aut-name=SakamotoTakeshi en-aut-sei=Sakamoto en-aut-mei=Takeshi kn-aut-name= kn-aut-sei= kn-aut-mei= aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Okayama University END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue= article-no= start-page=29 end-page=32 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Rat Peritoneal Mast Cells 2. Effect of BSA on Ca(2+) Uptake of Mast Cells kn-title=ラット腹腔肥満細胞に関する研究 第2報 : 肥満細胞のCa(2+)取りこみに対するBSAの影響について en-subtitle= kn-subtitle= en-abstract= kn-abstract=To examine the effect of BSA on the cell surface of mast cells, the (45)Ca influx was observed in mast cells from actively sensitized rats and also in cells passively sensitized with IgE-containing serum before or after purification by BSA. The mast cells from actively sensitized rats showed a marked increase in the (45)Ca uptake with stimulation by ovalbumin. The value of (45)Ca uptake by the cells was 1495 ± 104 cpm / 10(5) cells. The uptake of (45)Ca by mast cells passively sensitized before or after separation through BSA was also increased in response to ovalbumin, although the degree of 45Ca uptake by the latter showed marked variation. These results may suggest that BSA affects mast cell surface when the cells are sensitized after separation by BSA. en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=TanakaJuntaro en-aut-sei=Tanaka en-aut-mei=Juntaro kn-aut-name=田中淳太郎 kn-aut-sei=田中 kn-aut-mei=淳太郎 aut-affil-num=2 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=4 ORCID= en-aut-name=KishimotoTakumi en-aut-sei=Kishimoto en-aut-mei=Takumi kn-aut-name=岸本卓巳 kn-aut-sei=岸本 kn-aut-mei=卓巳 aut-affil-num=5 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=6 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=TownleyRobert G. kn-aut-sei=Townley kn-aut-mei=Robert G. aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学第2内科 affil-num=6 en-affil= kn-affil=岡山大学第2内科 affil-num=7 en-affil= kn-affil=クレイトン大学内科 END start-ver=1.4 cd-journal=joma no-vol=52 cd-vols= no-issue= article-no= start-page=23 end-page=28 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Rat Peritoneal Mast Cells 1. Purification of Mast Cells From Rat Peritoneal Cavity kn-title=ラット腹腔肥満細胞に関する研究 第1報 : 肥満細胞のpurification について en-subtitle= kn-subtitle= en-abstract= kn-abstract=To get a high recovery rate of mast cells from the rat peritoneal cavity, a new modified method was discussed using BSA density gradient. Three procedures, peritoneal lavage, washing of peritoneal cells and purification by BSA, were tested in this study, and the numbers of total cells and mast cells in each procedure were calculated. The recovery rate was the highest in BSA density gradient purification of the cells washed once with low speed centrifugation. The recovery rate was 73.7% and the number of mast cells taken from one rat was approximately 1.5×10(6). The purity of the cells with this procedure was 95.0% (range from 93.0 to 96.0%). en-copyright= kn-copyright= en-aut-name=TanizakiYoshiro en-aut-sei=Tanizaki en-aut-mei=Yoshiro kn-aut-name=谷崎勝朗 kn-aut-sei=谷崎 kn-aut-mei=勝朗 aut-affil-num=1 ORCID= en-aut-name=TanakaJuntaro en-aut-sei=Tanaka en-aut-mei=Juntaro kn-aut-name=田中淳太郎 kn-aut-sei=田中 kn-aut-mei=淳太郎 aut-affil-num=2 ORCID= en-aut-name=KomagoeHaruki en-aut-sei=Komagoe en-aut-mei=Haruki kn-aut-name=駒越春樹 kn-aut-sei=駒越 kn-aut-mei=春樹 aut-affil-num=3 ORCID= en-aut-name=MorinagaHiroshi en-aut-sei=Morinaga en-aut-mei=Hiroshi kn-aut-name=森永寛 kn-aut-sei=森永 kn-aut-mei=寛 aut-affil-num=4 ORCID= en-aut-name=KishimotoTakumi en-aut-sei=Kishimoto en-aut-mei=Takumi kn-aut-name=岸本卓巳 kn-aut-sei=岸本 kn-aut-mei=卓巳 aut-affil-num=5 ORCID= en-aut-name=KimuraIkuro en-aut-sei=Kimura en-aut-mei=Ikuro kn-aut-name=木村郁郎 kn-aut-sei=木村 kn-aut-mei=郁郎 aut-affil-num=6 ORCID= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=TownleyRobert G. kn-aut-sei=Townley kn-aut-mei=Robert G. aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=2 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=3 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=4 en-affil= kn-affil=岡山大学三朝分院内科 affil-num=5 en-affil= kn-affil=岡山大学第2内科 affil-num=6 en-affil= kn-affil=岡山大学第2内科 affil-num=7 en-affil= kn-affil=クレイトン大学内科 END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=71 end-page=84 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=リン脂質過酸化物の生物有機化学的合成 kn-title=Chemoenzymatic Synthesis of Phospholipid Hydroperoxides en-subtitle= kn-subtitle= en-abstract=健康正常人の血液などの組織中にはリン脂質過酸化物が極微量で存在し，疾病や老化によってその濃度が顕著に上昇する事が知られている．その事が明らかにされた当初は，極めて複雑な混合物をなす生体脂質中に含まれる不安定な極微量の過酸化脂質を単離・構造決定する事は殆ど不可能と考えられていた．現在もなお，そのような脂質過酸化物を生体組織から純粋に取り出し，構造決定したという報告は無い．従ってそのような分子種の化学的・生理学的性質は不明であったが，脂肪酸過酸化物が毒性を示す事から，リン脂質過酸化物もおそらく毒性を示すだろうと考えられてきた．このような漠然とした推定を科学的に明らかにするためには，化学合成によらざるを得ない．我々はこの未知の合成に取りかかった．しかし，従来の化学的手法のみでは不可能である事も明らかであった．その中で予想された困難の一つは極めて不安定なヒドロペルオキシ基を不飽和脂肪酸のある特定の位置にどのように導入するかという問題と，ヒドロペルオキシ基に影響を与える事なく合成中間体をどのように化学変換するかであった．第一の問題に対する解決策として，不飽和脂肪酸に大豆リポキシゲナーゼを作用させる事で解決する事ができた．植物に広く分布する酵素であるリポキシゲナーゼは植物中でリノール酸に作用して過酸化し，その生成物にヒドロペルオキシドリアーゼという酵素が作用して種々のアルデヒドが精製し，これは植物の青臭みを与える．第二の問題に対しては，リノール酸に導入された不安定なヒドロペルオキシ基をパーアセタールとしての保護する事により解決した．この保護基は，中間体から最終生成物に至るまでの反応条件，例えばDCCによるアシル化反応に対して安定である事が明らかとなった．この二つの問題を解決する事によって，リン脂質過酸化物の一つであるホスファチジルコリン過酸化物を世界に先駆けて成功した．さらにこのホスファチジルコリン過酸化物に微生物由来のホスフォリパーゼDを作用させる事によってホスファチジルエタノールアミン過酸化物，ホスファチジルセリン過酸化物やホスファチジルグリセロール過酸化物の合成にも成功した．また，トリグリセリド過酸化物の合成も可能になった．これらの脂質過酸化物が化学的に実態のあるものとして認識されてから，その生理作用に関する研究が広範に行われている．しかし，生体組織に存在するリン脂質過酸化物の生理学的役割は依然として明らかになっていない．ある種のリン脂質過酸化物が動物の免疫系を活性化するという報告もあり，必ずしも生体に対して悪い作用をするばかりではないようである． kn-abstract=Chemoenzymatic synthesis of 1-stearoyl-2-hydropeoxyacyl-sn-glycerophospholipids including phosphatidylcholine (PC-OOH), phosphatidic acid (PA-OOH), phosphatidylethanolamine (PE-OOH), phosphatidylglycerol (PG-OOH) and phosphatidylserine (PS-OOH). The hydroperoxy acyl moieties were prepared via hydroperoxidation of linoleic, dihomo-γ-linolenic and arachidonic acids by soybean, potate lipoxygenase or autoxidation. Their hydroperoxy group was protected as a dimethylperacetal before condensation with lysophosphatidylcholine. Optically active lysophosphatidylcholine was prepared via short pathway involving lipase-catalyzed direct enantioselective stearoylation of 2-O-benzylglycerol and choline phosphate synthesis. Peroxy fatty acids and lysophosphatidylcholine thus obtained were condensed using dicyclohexylcarbodiimide in chloroform. Removing the peracetal group in the product and purification by reverse-phase chromatography afforded the desired PC-OOH’s. PA-OOH, PG-OOH, PE-OOH and PS-OOH were obtained by phospholipase-D catalyzed transphosphatidylation from PC-OOH. As a reference compound for biological studies of hydroperoxy phopholipid, PC-OH's were also prepared in which hydroxy unsaturated fatty acyl group was linked to the sn-2 position of the glycerophospholipids. en-copyright= kn-copyright= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=1 ORCID= en-aut-name=YonedaKenji en-aut-sei=Yoneda en-aut-mei=Kenji kn-aut-name=米田健司 kn-aut-sei=米田 kn-aut-mei=健司 aut-affil-num=2 ORCID= en-aut-name=SasakuraKeiji en-aut-sei=Sasakura en-aut-mei=Keiji kn-aut-name=笹倉敬司 kn-aut-sei=笹倉 kn-aut-mei=敬司 aut-affil-num=3 ORCID= en-aut-name=ShigetaYasutami en-aut-sei=Shigeta en-aut-mei=Yasutami kn-aut-name=繁田泰民 kn-aut-sei=繁田 kn-aut-mei=泰民 aut-affil-num=4 ORCID= en-aut-name=KishidaYasuhiro en-aut-sei=Kishida en-aut-mei=Yasuhiro kn-aut-name=岸田靖弘 kn-aut-sei=岸田 kn-aut-mei=靖弘 aut-affil-num=5 ORCID= en-aut-name=AoishiAkihiro en-aut-sei=Aoishi en-aut-mei=Akihiro kn-aut-name=青石晃宏 kn-aut-sei=青石 kn-aut-mei=晃宏 aut-affil-num=6 ORCID= en-aut-name=DaidoHiroko en-aut-sei=Daido en-aut-mei=Hiroko kn-aut-name=大同浩子 kn-aut-sei=大同 kn-aut-mei=浩子 aut-affil-num=7 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=8 ORCID= en-aut-name=IwasaJunkichi en-aut-sei=Iwasa en-aut-mei=Junkichi kn-aut-name=岩佐順吉 kn-aut-sei=岩佐 kn-aut-mei=順吉 aut-affil-num=9 ORCID= en-aut-name=TaharaShoichi en-aut-sei=Tahara en-aut-mei=Shoichi kn-aut-name=田原正一 kn-aut-sei=田原 kn-aut-mei=正一 aut-affil-num=10 ORCID= en-aut-name=KanekoTakao en-aut-sei=Kaneko en-aut-mei=Takao kn-aut-name=金子孝夫 kn-aut-sei=金子 kn-aut-mei=孝夫 aut-affil-num=11 ORCID= en-aut-name=MatsuoMitsuyoshi en-aut-sei=Matsuo en-aut-mei=Mitsuyoshi kn-aut-name=松尾光芳 kn-aut-sei=松尾 kn-aut-mei=光芳 aut-affil-num=12 ORCID= en-aut-name=ShimizuSakayu en-aut-sei=Shimizu en-aut-mei=Sakayu kn-aut-name=清水昌 kn-aut-sei=清水 kn-aut-mei=昌 aut-affil-num=13 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=岡山大学 affil-num=8 en-affil= kn-affil=岡山大学 affil-num=9 en-affil= kn-affil=岡山大学 affil-num=10 en-affil= kn-affil=東京都老人総合研究所 affil-num=11 en-affil= kn-affil=東京都老人総合研究所 affil-num=12 en-affil= kn-affil=東京都老人総合研究所 affil-num=13 en-affil= kn-affil=京都大学農学部 en-keyword=phospholipid kn-keyword=phospholipid en-keyword=peroxide kn-keyword=peroxide en-keyword=hydroperoxide kn-keyword=hydroperoxide en-keyword=phospholipase D kn-keyword=phospholipase D en-keyword=lipoxygenase kn-keyword=lipoxygenase END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=1 article-no= start-page=7 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=2010 dt-pub=20100201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of Cystathionine γ-Synthase from Thermoacidophilic Archaea Sulfolobus tokodaii kn-title=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニン γ-シンターゼの精製及び性質検討 en-subtitle= kn-subtitle= en-abstract=好熱好酸性アーキア Sulfolobus tokodaii 由来シスタチオニンγﾝシンターゼ（stCGS）遺伝子を pET-11a に組み込み pET-stCGS を構築した．このベクターでE. coli Rosettaﾝgami（DE3）を形質転換し，本遺伝子を発現させ，精製及び性質検討を行った．大腸菌で発現したシスタチオニンγﾝシンターゼの活性が無細胞抽出液で確認できた．S. tokodaii シスタチオニンγﾝシンターゼを70℃熱処理 DEAEﾝトヨパールイオン交換カラム等により単一精製した．精製酵素の最適温度は100℃以上であり，熱安定性は60分間処理で70℃までほぼ100ｵの残存活性を示した．また，最適pHについてはリン酸緩衝液やブリトンﾝロビンソン広域緩衝液の場合はpH7.0の時が最も活性が高く，トリス塩酸緩衝液の場合はpH9.0が最適であった．pH安定性についてはpH5.0〜9.0において安定であった．O-ホスホ-l-ホモセリンに対するKm，Vmaxは，それぞれ0.82mM，2.42U/rであった．アポ酵素のホロ化実験により，本酵素活性がPLP に依存していることが明らかとなった．更に本酵素の脱離反応での基質特異性の検討を行った．変異酵素を用いた実験により，stCGSの基質特異性には，活性中心に存在するPhe97を含む領域が深く関わっていることが示唆された． kn-abstract=The gene encoding a cystathionine γ-synthase from Sulfolobus tokodaii was cloned and expressed in Escherihia coli Rosetta-gami (DE3). Cystathionine γ-synthase ［EC 2. 5. 1. 48］ from Sulfolobus tokodaii (stCGS) was purified by heat treatment, DEAE- Toyopearl 650M and Sephacryl S-300 column chromatographies from E. coli transformants. stCGS shows optimum activity at pH 7.0, and is stable between pH5.0 and pH9.0. The optimum temperature of stCGS is above 100℃, and the enzyme showed the remaining activity of almost 100% up to 70℃. The K(m) and V(max) with O-phospho-L- homoserine as a substrate are 0.82 mM and 2.42 U/mg. To analyze the role of Phe 97 in the active site of stCGS, we constructed F97Y, R99C, and F97Y-R99C mutant enzymes. Although native stCGS has no activity toward l-methionine, F97Y mutant enzyme gained the elimination activity toward L-methionine. en-copyright= kn-copyright= en-aut-name=ShinozakiMai en-aut-sei=Shinozaki en-aut-mei=Mai kn-aut-name=篠崎舞 kn-aut-sei=篠崎 kn-aut-mei=舞 aut-affil-num=1 ORCID= en-aut-name=YanagitaniMasahiko en-aut-sei=Yanagitani en-aut-mei=Masahiko kn-aut-name=柳谷昌彦 kn-aut-sei=柳谷 kn-aut-mei=昌彦 aut-affil-num=2 ORCID= en-aut-name=KanedaShouichirou en-aut-sei=Kaneda en-aut-mei=Shouichirou kn-aut-name=兼田翔一郎 kn-aut-sei=兼田 kn-aut-mei=翔一郎 aut-affil-num=3 ORCID= en-aut-name=KudouDaizou en-aut-sei=Kudou en-aut-mei=Daizou kn-aut-name=工藤大蔵 kn-aut-sei=工藤 kn-aut-mei=大蔵 aut-affil-num=4 ORCID= en-aut-name=EndouYuuichi en-aut-sei=Endou en-aut-mei=Yuuichi kn-aut-name=遠藤祐一 kn-aut-sei=遠藤 kn-aut-mei=祐一 aut-affil-num=5 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=6 ORCID= en-aut-name=KuramitsuSeiki en-aut-sei=Kuramitsu en-aut-mei=Seiki kn-aut-name=倉光成紀 kn-aut-sei=倉光 kn-aut-mei=成紀 aut-affil-num=7 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=8 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 affil-num=7 en-affil= kn-affil=大阪大学大学院理学研究科 affil-num=8 en-affil= kn-affil=岡山大学 en-keyword=cystathionine γ-synthase kn-keyword=cystathionine γ-synthase en-keyword=pyridoxal 5’-phosphate kn-keyword=pyridoxal 5’-phosphate en-keyword=thermoacidophilic archaea kn-keyword=thermoacidophilic archaea en-keyword=Sulfolobus tokodaii kn-keyword=Sulfolobus tokodaii END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=5-2 article-no= start-page=2685 end-page=2701 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590425 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Electron Microscopic observation on Rickettsia tsutsugamushi kn-title=恙虫病リケッチャの電子顕微鏡的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The author tried purification of Rickettsia tsutsugamushi, and electron microscopic observation of the purified rickettsiae, and also studied electron microscopic figures of the ultrathin sections of the mouse organs and tissures infected with Rickettsia tsutsugamushi. The results are summarized as follow: 1) Both of the rickettsiae purified and observed in the ultra-thin sections of the abdominal exsudate cells were of various figures. The size of purified rickettsiae is 0.6 to 1.0μ, and that of those in abdominal cells is 0.3 to 0.5μ on the average. 2) A capsul-like substance could be observed around the purified rickettsiae, but the presence of such substance could not be proved around the ones in abdminal cells. 3) In more than a half of the rod-shaped purified rickettsiae, the so-called polarstaining-like structure could he observed of those in abdminal cells, some has the polarstaining like structure, but most of them were of no or somewhat granular structure. 4) In ultra-thin sections of abdminal cells, the presence of rickettsiae could be clearly obsered. In the liver and spleen particularly in the liver, the rickettsialike structures could be also observed, but the identification needs further studies, en-copyright= kn-copyright= en-aut-name=NigumaKoichiro en-aut-sei=Niguma en-aut-mei=Koichiro kn-aut-name=仁熊浩一郎 kn-aut-sei=仁熊 kn-aut-mei=浩一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部微生物学教室 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=2-2 article-no= start-page=849 end-page=865 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Extraction Substances of Dysentery Bacillus by Means of Middlebrook's Hemolytic Reaction 1. Comparative Study on Chemical Natures of Extraction Substances 2. Comparative Study on Hemolytic Mature of Extraction Substances 3. Hemolytic Nature of Diluted Antigens kn-title=Middlebrookの溶血反応による赤痢菌体抽出物質の研究 1) 菌体抽出物質の化学的性状の比較 2) 菌体抽出物質の溶血性状の比較 3) 抗原溶液の濃度別に見た溶血性状 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Of Various extraction substances of Shigella sonnei, the author studied the chemical natures, the hemolytic specificity and the hemolysis by various diluted fractions, and, in addition to these, comparatively studied the natures of various extraction substances of S and R types. The results are briefly summarized as follows: 1) As for the chemical natures of various fractions, particularly of nucleic acids, the nucleic acid extracted with phenol was fairly purer than nucleic acid II extracted with saline and weak alkaline solutions and deproteinized by chloroform-gel and trypsin digestion method. 2) In the hemolysis, hemolytic titer of nucleic acid group substances rised with the progress of purification procedures; this fraction was considered to be a significant one as the antigen. 3) In the hemolysis by various concentration of the antigen, no difference was observed between those of chick and rabbit red cells by the 1mg/ml purified nucleic acid II, but a clear differnce existed between the two by 10γ/ml of it. Chick red cells were better sensitized by nucleic acid group substances than Boivin's substance, and rabbit red cells showed a reversse relationship. 4. As for the S and R type difference, polysaccharide substances showed a specific difference; the CF and Boivin's substances of S type showed a stronger red cell sensitization those of R type. en-copyright= kn-copyright= en-aut-name=NakayamaGoro en-aut-sei=Nakayama en-aut-mei=Goro kn-aut-name=中山五郎 kn-aut-sei=中山 kn-aut-mei=五郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部微生物学教室 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=7 article-no= start-page=2307 end-page=2320 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=1958 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Virus of Infectious Hepatitis in Okayama Prefecture T: Purification of the Isolated Virus U: Electron Microscopic Study on the Isolated Virus kn-title=岡山県下に発生した流行性肝炎の病原体に関する研究 i) 分離ウイルスの精製 ii) 分離ウイルスの電子顕微鏡的観察 en-subtitle= kn-subtitle= en-abstract=Purification and electron microscopic study of the virus isolated by Murakami et al. from the patients of infectious hepatitis in Okayama Prefecture were tried in parallel with animal experiments. The results are summarized as follows: 1) Of all the purification methods tried, the combination of infusion with a saline solution and centrifugation was proved to be the most excellent one for electron microscopic observation and animal experiments. The good efficiency and the simplicity of its procedures also made this method suitable for the purification of the virus of infectious hepatitis. 2) In electron micrographs, the viruses were observed as round or ovoid particles of abo t 50 mμ in diameter. 3) Inoculation of the material containing these particles into mice caused such pathological findings as previously reported by Murakami at el.; a high degree of degeneration or necrosis of liver parenchymal cells accompanied with various cell infiltrations. These observations led the author to the conclusion that the purified particles were nothing but the isolated hepatotropic viruses. kn-abstract= en-copyright= kn-copyright= en-aut-name=HashimotoRyohei en-aut-sei=Hashimoto en-aut-mei=Ryohei kn-aut-name=橋本良平 kn-aut-sei=橋本 kn-aut-mei=良平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=71 cd-vols= no-issue=1 article-no= start-page=163 end-page=167 dt-received= dt-revised= dt-accepted= dt-pub-year=1959 dt-pub=19590131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Electron-Microscopic Study on the Agglutination Reaction with Purified Bacterial Flagella kn-title=精製細菌鞭毛に依る凝集反応の電子顕微鏡的観察法に就て en-subtitle= kn-subtitle= en-abstract= kn-abstract=The flagella of Bacillus Proteus were purified according to Fukumi's method. To these purified flagella, antiserum and human γ-globulin were added, and the morphological change was electron microscopically observed. The results were briefly summarized as follows: 1) For the purification of flagella, homogenation for 15 seconds is the best. 2) The purified flagella of Bacillus proteus had a rope structure. 3) After the addition of antiserum, agglutination figure of flagella was electron-microscopically observed. 4) The enlargement of flagella, however, could not be observed after the addition of antiserum. 5) The grade of agglutination of flagella goes in parallel with the grade of dilution of antiserum 6) The addition of human γ-globulin did not cause agglutination, and there was observed only the adsorbing figure of globulin particles on them. en-copyright= kn-copyright= en-aut-name=ShidaTsuguo en-aut-sei=Shida en-aut-mei=Tsuguo kn-aut-name=志田次夫 kn-aut-sei=志田 kn-aut-mei=次夫 aut-affil-num=1 ORCID= en-aut-name=FukushimaSatoshi en-aut-sei=Fukushima en-aut-mei=Satoshi kn-aut-name=福島里志 kn-aut-sei=福島 kn-aut-mei=里志 aut-affil-num=2 ORCID= en-aut-name=IshiiSeiichi en-aut-sei=Ishii en-aut-mei=Seiichi kn-aut-name=石井誠一 kn-aut-sei=石井 kn-aut-mei=誠一 aut-affil-num=3 ORCID= en-aut-name=NakashimaTadaatsu en-aut-sei=Nakashima en-aut-mei=Tadaatsu kn-aut-name=中島忠厚 kn-aut-sei=中島 kn-aut-mei=忠厚 aut-affil-num=4 ORCID= en-aut-name=NikumaKohichiro en-aut-sei=Nikuma en-aut-mei=Kohichiro kn-aut-name=仁熊浩一郎 kn-aut-sei=仁熊 kn-aut-mei=浩一郎 aut-affil-num=5 ORCID= en-aut-name=SugaMasao en-aut-sei=Suga en-aut-mei=Masao kn-aut-name=菅昌夫 kn-aut-sei=菅 kn-aut-mei=昌夫 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部微生物学教室 affil-num=2 en-affil= kn-affil=岡山大学医学部微生物学教室 affil-num=3 en-affil= kn-affil=岡山大学医学部微生物学教室 affil-num=4 en-affil= kn-affil=岡山大学医学部微生物学教室 affil-num=5 en-affil= kn-affil=岡山大学医学部微生物学教室 affil-num=6 en-affil= kn-affil=岡山大学医学部微生物学教室 END start-ver=1.4 cd-journal=joma no-vol=70 cd-vols= no-issue=1 article-no= start-page=197 end-page=209 dt-received= dt-revised= dt-accepted= dt-pub-year=1958 dt-pub=19580131 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Rickettsia tsutsugamushi T: Experiments on the Purification of Rickettsia tsutsugamushi kn-title=恙虫病病毒に関する研究 第1編 恙虫病病毒の精製に関する実験 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In the present experiments, Mitani and Tanizawa strains, which had been isolated in Kagawa Prefecture, were used. Rickettsiae of each strain were purified with kaolin and bentonite. The results were as follows: 1) In the purification of rickettsiae with kaolin, the adsorption on kaolin at pH 5.0 and the dissociation at pH 7.2 gave the purified rickettsiae in the largestest quantity, and thus purified rickettsiae showed a high antigenicity in the complement fixation test. 2) In the purification with bentonite, the adsorption at pH 4.5 and the dissociation at pH 7.2 gave the purified rickettsiae in the richest amount, and the purified rickettsiae thus obtained showed a high antigenicity in the complement fixation test. en-copyright= kn-copyright= en-aut-name=OhnishiYuzuru en-aut-sei=Ohnishi en-aut-mei=Yuzuru kn-aut-name=大西譲 kn-aut-sei=大西 kn-aut-mei=譲 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部微生物教室 END start-ver=1.4 cd-journal=joma no-vol=75 cd-vols= no-issue=10 article-no= start-page=923 end-page=934 dt-received= dt-revised= dt-accepted= dt-pub-year=1963 dt-pub=19631030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on Viral Tumors cultivated in the embryonated Egg Part U. Cultivation of Viral materials isolated from mouse leukemia and mammary cancer kn-title=ウイルス性腫瘍の発育鶏卵内培養に関する研究 第2編 マウス白血病並びに乳癌より得られたウイルス材料の発育鶏卵内培養 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The viral materials isolated from the spleen of C58 strain mice with myelogenous and lymphocytic leukemia were inoculated on the chorio-allantoic membrane, in the allantoic cavity and in the yolk sac of embryonated eggs. Purification of the viral materials was done by filtration through Berkefeld N filters or emulsification with fluorocarbon. From the observations on the changes of the chorio-allantoic membrane and on the influence of the inoculation to chick embryos, the following results are obtained. 1) Inoculation on the chorio-allantoic membrane. Inoculation of the filtered viral materials caused slight thickning, hyperemia, opacities and edema of the inoculated areas of the chorio-allantoic membrane. but no changes were observed in other embryonic organs. Histologically, the chorio-allantoic mambrane showed proliferation of fibroblasts and epithelial cells and infiltration of monocytoid cells and eosinophils in the interstitial tissue. In the inoculation of the fluorocarbon-purified viral materials from myelogenous and lymphocytic leukemia, the changes of the chorioallantoic membrane were almost the same as findings described above. But in the case of mammary cancer, changes such as thickning, opacities, hyperemia and edema of the inoculated areas of the chorioallantoic membrane were more remarkable and sometimes hemorrhages and tumor-like nodular swellings were also observed. Histologically, proliferation of epithelial cells, fibroblasts and infiltration of eosinophils, lymphoid and monocytoid cells were much more prominent. No pathologic changes of other embryonic organs were observed following inocuration of the viral materials isolated by either method. 2) Inoculation in the allantoic cavity. In all cases, no significant organ changes were observed and no death of the embryos occurred. 3) Inoculation in the yolk sac. Inoculation of the filtered viral materials caused 100 % desth of the embryos by the ninth day after inoculation in the case of myelogenous leukemia, by the twelfth day in lymphocytic lukemia and by the fifth day in mammary cancer. On the other hand, in the inoculation of the fluorocarbon-purified viral materials, 100 % mortality occurred by the fifth day in myelogenous leukemia and mammary cancer and by the ninth day in lymphocytic leukemia. These varied experimental results are considered to be related to the differences in strains and virulencies of the viruses. en-copyright= kn-copyright= en-aut-name=IshihamaShinzi en-aut-sei=Ishihama en-aut-mei=Shinzi kn-aut-name=石濱真治 kn-aut-sei=石濱 kn-aut-mei=真治 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=7 article-no= start-page=1121 end-page=1127 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=19650730 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Study on Japanese B encephalitis virus by means of electoron microscopy and fluorescent antibody method. 1. A study on purification of Japanes B encephalitis virus by fluorocarbon deproteinization and electoron microscopic observation on the virus particle kn-title=日本脳炎ウイルスの電子顕微鏡及び螢光抗体法による研究 第1編 日本脳炎ウイルス精製とウイルス粒子の電子顕微鏡像 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The fluorocarbon deproteinization technique was demonstrated to be a useful method for the purification of Japanese B encephalitis (JE) virus from infected mouse brain. Electoron microscopic observation on purified virus particles by this method schowed numerous particles which were presumed to be JE virus. Electoron microscopic observation on the surface of the chick red cells which were precipitated with purified virus (hemagglutination) displayed similar particles. The JE particles were hexogonal in schape, and composed of an outer membranes and a centrally located nucleoid-like structure. The size of the particle measured 30 to 40 mμ in diameter. A substance which cause the hemagglutination was conservable to be the virus particle itself. en-copyright= kn-copyright= en-aut-name=TakahashiKenji en-aut-sei=Takahashi en-aut-mei=Kenji kn-aut-name=高橋建次 kn-aut-sei=高橋 kn-aut-mei=建次 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=77 cd-vols= no-issue=3 article-no= start-page=551 end-page=570 dt-received= dt-revised= dt-accepted= dt-pub-year=1965 dt-pub=19650330 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on surface structures of the bacterial cell Report 111. Chemical studies on surface substances of the bacterial cell kn-title=細菌表面構造の研究 第3編 細菌表面構成物質の化学的研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Surface substances of the bacterial cell which would have biological and immunological activities were studied with aid of purification process, chemical analysis, spectrochemical procedures, viscosimetory, dielectric spectrometory, osmotic manometory and other physicochemical procedures. Purified substance from Gram positive bacterial cell by trichlor-acetic acid method was determined to be the mixture of ribonucleic acid-Mg and polysaccharde-polypeptide-lipid. Mixture was consisted of ion-binding in one-fifth and its molecular weight was ca. 400,000 with axis ratio of 1:2. By acting with ribonuclease this mixture was purified into polysaccharide-polypeptide-lipid compound which, to date, had been difficult to purify from Gram positive bacterial cell. Purified polysaccharide-polypeptide-lipid compound from Gram negative bacterial cell using trichlor-acetic acid method was also studies revealing molecular weight of ca. 150,000 and twice longer axis ratio than ribonucleic acid mixture. Thus, it was considered that parallel coexistence of two molecules of ribonucleic acid-Mg and one molecule of polysaccharide polypeptide-lipid compound was clarified. Polysacchardie-polypeptide-lipid compound was further purified into polysaccharide-polypeptide 1 (PP 1), polysaccharide-polypeptide 11 (PP 11) and lipid. From measuring molecular weight, km constant, axis ratio etc, it was found that PP 1 had more elongated molecular form than PP 11 and that relationship between PP 1 and PP 11 was identical with that between non-denaturated protein and denaturated protein. en-copyright= kn-copyright= en-aut-name=TaguchiKazumi en-aut-sei=Taguchi en-aut-mei=Kazumi kn-aut-name=田口一美 kn-aut-sei=田口 kn-aut-mei=一美 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部細菌学教室 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=11-12 article-no= start-page=1091 end-page=1103 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19681230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on (90)YCl(3) Colloid for Clinical Use Part I. Purification of (90)YCl(3) Colloid by Milking Method and the Observation of Electrone Microscopy kn-title=(90)YCl(3)コロイドに関する基礎的研究 第1編 1)イオン交換樹脂法(milking system)による(90)YCl(3)分離採取時の混入(90)Srの検出ならびに除去に関する研究 2)各種(90)YCl(3)コロイドの電顕的観察について en-subtitle= kn-subtitle= en-abstract= kn-abstract=(90)Y is a high-energy radioisotope and emite β-ray with 2.6 days half life. (90)YCl(3) colloid has been used for malignant tumor therapeutically, as (198)Au colloid. In the present experiment, the purification of (90)Y, and the detection of the contaminated (90)Sr was studied. Amberlite XE-100 column was suitable for this prupose. The colloidal size of (90)YCl(3) was transformed by pH, and the features of (90)YCl(3) colloids were observed on electrone microscope. 1) (90)Y and (90)Sr mixture adsorbed on Amberlite XE-100 column (100-200 mesh, 2.5 column volume), and eluted by 0.5 N HCl. (90)Sr is eluted out at 10-60 ml, and (90)Y is eluted out from 170 ml. 2) A mixture of 20 mC of citrate-(90)Y complex and 1μC of (90)Sr fixed on this column, and then eluted by 0.5 N HCl. One drop of eluate at 10 ml was detectable by GM counter. The detectable lower limit of contaminated (90)Sr is 0.1μC by re-counting on GM counter 24 hrs after the elution. 3) Amberlite XE-100 column is suitable for the second column on the milking system, for the purpose of separating the contaminated (90)Sr and (90)Y. This procedure is complete in 1 hr process. 4) (90)YCl(3) colloid is aggregated and formed clamp in high pH solution, Electrone microscopic observations showed that the colloidal size was 40-50 mμ at pH 5, and 20 mμ at pH7. en-copyright= kn-copyright= en-aut-name=TachibanaAkihisa en-aut-sei=Tachibana en-aut-mei=Akihisa kn-aut-name=立花明久 kn-aut-sei=立花 kn-aut-mei=明久 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1外科教室 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1-2 article-no= start-page=95 end-page=102 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the Relationship between Gastric Ulcers and FungiPart 2. Studies on the Relationship Between Experimental Gastric Ulcers and Fungi kn-title=胃潰瘍と真菌に関する研究 第U編 実験的胃潰瘍と真菌感染に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Studies were performed on the relationship between hard cured gastric ulcers and fungi in rats. Experimental gastric ulcers were prepared by the use of the Clamping-Cortisone method. Rats with experimental gastric ulcers were divided into 3 groups, A, B and C. Group A was neither administered drugs nor fungi. Group B was given Candida albicans. Group C was dosed chlorhexidine. The results were as follows: 1. Experimental gastric ulcers with fungi in their bottom were found in 4 rats out of 13 belonging to group B. 2. The defection of fungi on the crate surface of the experimental gastric ulcers revealed that positive results were obtained in all cases of group A or B and in 3 cases out of 11 of group C. As to the species of the fungi, in group a Torulopsis famata, in group B Candida albicans and in group C. Torulopsis famata were identified respectively. 3. The normal fungous flora in the intact stomachs of experimental animals was identified as Torulopsis famata. 4. The fungous form in the bottom of experimental gastric ulcers was yeastlike in the cases that were sacrificed 3 weeks later making ulcers. In case the heading of experimental gastric ulcers was protracted, there were seen mycerial fungi increasing in quantity. 5. The crater surface of the gastric ulcers of group B where fungi grew abundantly was dirty histlogically and the recovery of gastric ulcers was delaye l. The scar formation of gastric ulcers of group B was weaker than group A and C. These results apparently indicate that the course of the gastric ulcers with fungi in their bottom will be delayed in group B. 6. The recovery of experimental gastric ulcers in group C was most remarkable among 3 groups. It may be posible that the application of chlorhexidine as the gastric ulcer agent will contribute to the purification of the surface of gastric ulcers and promote the healing of them. en-copyright= kn-copyright= en-aut-name=KitaSchoichi en-aut-sei=Kita en-aut-mei=Schoichi kn-aut-name=北昭一 kn-aut-sei=北 kn-aut-mei=昭一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科教室 END start-ver=1.4 cd-journal=joma no-vol=80 cd-vols= no-issue=1-2 article-no= start-page=1 end-page=12 dt-received= dt-revised= dt-accepted= dt-pub-year=1968 dt-pub=19680228 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Immunofluorescent Studies on Viral Antigens of the Mammary Cancer in C(3)H Mice. T. Purification Study of Viral Antigens in C(3)H Mouse Mammary Cancer and its Evaluation by Direct Immunofluorescent Staining kn-title=C(3)Hマウス乳癌におけるウイルス抗原の螢光抗体法による研究 第1編 C(3)Hマウスにおける乳癌ウイルス抗原の精製とその螢光抗体法直接法による検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Virus preparations were obtained from mammary tumors which developed spontaneously in C(3)H mice by three different methods; 1) filtration through Berkefeld-N or Chamberland L(3) filters, 2) differential centrifugations, and 3) fluorocarbou extraction. Each virus preparation was used for immunizing rabbits, and was then evaluated for the degree of purification of the viral antigens by direct immunofluorescent staining. Following results were obtained: 1. The fluorocarbon extractin method was more excellent for the purification of mammary cancer virus than filtration or differential centrifugation method. 2. Staining procedure of the direct immunofluorescence was improved as follows; frozen sections were fixed with cold acetone-fluorocarbon mixture instead of coid acetone, and duration of staining was prolongel over six hours in a cold moist chamber. As a result it was possible to observe clearer and more numerous fluorescence than by the direct staining of Coons' original procedure. en-copyright= kn-copyright= en-aut-name=MakihataToru en-aut-sei=Makihata en-aut-mei=Toru kn-aut-name=巻幡徹 kn-aut-sei=巻幡 kn-aut-mei=徹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科 END start-ver=1.4 cd-journal=joma no-vol=86 cd-vols= no-issue=9-10 article-no= start-page=499 end-page=512 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=19741030 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Virological Studies on Human Leukemia Report T. Analysis of Viral Antigens of Rauscher Murine Leukemia andHuman Leukemia Cells by Direct Immunofluorescence with Anti-Rauscher Leukemia Virus Serum kn-title=人白血病のウイルス学的研究 第1編 Rauscher白血病ウイルス抗血清を用いた蛍光抗体法によるRauscherマウス白血病細胞および人白血病細胞のウイルス抗原の検索 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Specificity of anti-Rauscher leukemia virus goat serum was strictly checked by purification, labelling with fluorescein and elimination of nonspecific reaction. Using this antiserum, antigen analyses of the spleen and liver cells of Rauscher leukemic mice and human leukemic cells were examined by the direct immunofluorescence technique and following results were obtained.By electron microscopy, the eclipse phase was reported to be about 1 week after virus inoculation, but it was found that viral antigen was observable already in three days after virus infection. In the experiment with human leukemias, cells from bone marrow or peripheral bloodsmears from patients with 20 acute leukemias (14 of myelocytic, 6 of lymphocytic), 6 chronic leukemias (3 of myelocytic, 3 of lymphocytic), 10 malignant lymphomas (4 of Hodgkin disease, 4 of lymphosarcoma, 2 of reticulum cell sarcoma), 2 multiple myeloma and 10 other disorders were tested with anti-Rauscher leukemia virus goat serum. However, specific fluorescence was not seen in any cells tested. Based on these findings, the role of virus in human leukemia was discussed. en-copyright= kn-copyright= en-aut-name=UnoJunichiro en-aut-sei=Uno en-aut-mei=Junichiro kn-aut-name=宇野潤一郎 kn-aut-sei=宇野 kn-aut-mei=潤一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科 END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=7-8 article-no= start-page=553 end-page=570 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=19760830 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the antigenicity of lymphocyte membrane PartU Immunizing experiment on mice kn-title=リンパ球膜の抗原性に関する研究 第2編 マウス感作実験 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Further investigation concerned on the antigenicity of the lymphocyte membrane was performed in this paper using C57B1 mice and purified lymphocyte membranes which were preparated from either calf thymic lymphocyte (TM) or bovine intestinal lymphnode lymphocyte (LM). The detail of the methods for the purification of lymphocyte membrane were described in the part T of this article. Intraperitoneal injection of 5, 10 or 100γ of lymphocyte membrane was performed once a week untill 5 weeks. The peripheral blood pictures, diameter of lymphocyte, histological studies on the thymus, lymphnode and spleen, serum protein electrophoresis, immunoelectrosyneresis, RFC and PHA reactivity were examined in these immunized mice. The lymphocytes both in the peripheral blood and lymphatic organs were diminished profoundly in the mice immunized with TM. Especially the lymphocytes in the T dependent area of spleen and lymphnode as well as in the cortical area of the thymus decreased intensively in contrasting with a decrease of B cell area of spleen and lymphnode in the mice immunized with LM. The lymphocyte depletion in the lymphoid organs of the mice immunized with 100γ LM had a similar pattern to those of mice immunized with TM indicating the contamination of TM into the LM preparation up to approximately 6-8%. Temporary increase of α1-globulin, α2-globulin and α3-globulin in this order was noted in serum of the mice immunized with TM, being followed by hypergammaglobulinemia. On the other hand an increase of α3-globulin and then of γ-globulin appeared rapidly in the mice immunized with LM. The discrepancies on the tissue reaction and protein fraction of these two groups suggested the difference of antigenicity between TM and LM on the activation of antibody-forming system. en-copyright= kn-copyright= en-aut-name=KobashiHidehiro en-aut-sei=Kobashi en-aut-mei=Hidehiro kn-aut-name=小橋秀広 kn-aut-sei=小橋 kn-aut-mei=秀広 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科教室 END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=5-6 article-no= start-page=427 end-page=438 dt-received= dt-revised= dt-accepted= dt-pub-year=1976 dt-pub=19760630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the antigenicity of lymphocyte membranePart T. The purification method of lymphocyte membraneand its immunization experiment on the rabbit kn-title=リンパ球膜の抗原性に関する研究 第1編 リンパ球膜の分離・精製法の検討と家兎感作実験 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The lymphocyte membrane was isolated from the calf thymocytes and the bovine intestinal lymphnode lymphocytes through a pretreatment with ZnCl(2) and Tween-20 combinig with a freeze-thawing procedure been followed by sucrose density gradient centrifugations. The recovery of lymphocyte membrane from the original lymphoid organ weight was 0.6 per cent (dry weight) and 0.9 per cent, as the lymphnode lymphocyte membrane (LM) and the thymic lymphocyte membrane (TM), respectively. Protein content in these preparations were 7.8 per cent and 2.3 per cent, in the LM and the TM, respectively. An amino acid content in the TM showed increased valine and leucine levels than the LM. Gel filtrations, using a Sepharose 4B column, of solubilized lymphocyte membranes revealed that the TM had higher protein content of larger molecular fractions than the LM. Disk electrophoresis also differentiated polypeptide bands between the LM and the TM. The morphological studies on these membrane preparations by a phase contrast microscope and an electron microscope demonstrated the purity of the membranes which was free from the contaminations of the microorganella. A rabbit was immunized with either one of lymphocyte membrane (TM of LM) with (intracutaneously) or without (intravenously) Freund's complete adjuvant. The circulating lymphocytes decreased more rapidly after the immunization of the TM than of the LM. The thymus, spleen, and lymphnode were atrophic macroscopically in the both groups. Histologically, however, more reduced lymphocytes in the tissue were observed in the group treated with the TM resulting a reduced cortical area with increased fatty degeneration in the thymus, been combined with a reduced lymphfollicular area in the spleen as same as the lymphnode than the group treated with the LM. Although a further elucidation is necessary, the lymphocyte membrane has shown specific antigenicity depending on the origin of the membrane upon the heterologous lymphoid organs. en-copyright= kn-copyright= en-aut-name=KobashiHidehiro en-aut-sei=Kobashi en-aut-mei=Hidehiro kn-aut-name=小橋秀広 kn-aut-sei=小橋 kn-aut-mei=秀広 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学平木内科教室 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=7-8 article-no= start-page=999 end-page=1013 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and properties of a high molecular weight acid soluble nuclear protein from mouse ascites sarcoma cells kn-title=マウス腹水肉腫細胞核からの高分子量酸可溶性蛋白質の精製とその性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A high molecular weight acid soluble nuclear protein (HAN-1) was isolated in a electrophoretically homogeneous state from mouse ascites sarcoma cells (SR-C3H cells) by the exclusion chromatography in Sephacryl S-200 and the DEAE-Sephadex chromatography of the nuclear 0.2M H(2)SO(4) extract. The content of HAN-1 in SR-C3H nuclei was about 4.3% per total histone, the highest among the cell types tested. The molecular weight of HAN-1 was estimated to be 125,000 by SDS-polyacrylamide gel electrophoresis. The amino acid composition of HAN-1 was rich in glutamic acid, alanine, lysine and aspartic acid, the acidic/basic amino acid ratio 1.8. The in vitro RNA synthesizing activity of SR-C3H cell RNA polymerases T and U on a naked DNA template was differentially affected by HAN-1; the reaction with polymerase I was inhibited and that with polymerase U stimulated. en-copyright= kn-copyright= en-aut-name=TsutsuiKimiko en-aut-sei=Tsutsui en-aut-mei=Kimiko kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 END start-ver=1.4 cd-journal=joma no-vol=87 cd-vols= no-issue=5-6 article-no= start-page=451 end-page=462 dt-received= dt-revised= dt-accepted= dt-pub-year=1975 dt-pub=19750630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Experimental and clinical studies on the catecholamine metabolism 1. Studies on the determination of catecholamines in tissues, serum, urine and cerebrospinal fluid by gas chromatography (ECD). kn-title=カテコールアミン代謝に関する基礎的ならびに臨床的研究 第T編 ガスクロマトグラフィー（ECD）法による組織，血液，尿および髄液中のカテコールアミン類の測定法についての研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Catecholamine in tissue, serum and urine has been determined by the fluorometric method. It still remains some problems, especially sensitivity in the measurement and purification of catecholamines according to this method. Therefore, I determined by a gas chromatographic method (using an Electron Capture Detector: ECD) modified from the method of Imai (1971). Catecholamines in cerebrospinal fluid (CSF) have not been determined by gas chromatographic method so far. A new technique for the determination of catecholamine in CSF of human will be presented in this paper. The results are as follows, 1) According to the gas chromatography, 20mg of tissue, 2.5ml of serum and 3.0ml of CSF are enough to determine with the grade of one nanogram. 2) Dopamine, norepinephrine, epinephrine, 3, 4-dihydroxyphenylalanine (DOPA), tyramine, octopamine, metanephrine, normetanephrine and synephrine were determined. Retention time of gas chromatography for these substances was decided. Heptachlor epoxide or dieldrin were available to the internal standard substances. 3) Dopamine was determined in serum of normal human subjects and in experimental animals (rat and cat). 4) Epinephrine was detected in brain tissue of rats and cats. This evidence will show that the central nervous system contains enough epinephrine to evaluate. 5) Micro-quantitative analysis of catecholamine and its derivatives in various samples will contribute to the neurochemical researches and to the clinical works in the central nervous system. en-copyright= kn-copyright= en-aut-name=KishikawaHidemi en-aut-sei=Kishikawa en-aut-mei=Hidemi kn-aut-name=岸川秀実 kn-aut-sei=岸川 kn-aut-mei=秀実 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設病態生化学部門 END start-ver=1.4 cd-journal=joma no-vol=90 cd-vols= no-issue=7-8 article-no= start-page=841 end-page=849 dt-received= dt-revised= dt-accepted= dt-pub-year=1978 dt-pub=197808 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the cornin extracted from liver 2. Purification of the rat liver microsomal cornin and its chemical properties kn-title=細胞分裂抑制物質肝コルニンの研究 2. ラット肝小胞体コルニン画分の分離精製とその化学的性質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A curude cornin extracted from rat liver microsomes and the chemical properties of the purified cornin (MF1) were investigated. 1. Crude rat liver microsomal cornin (RLMC) inhibits the growth of L-929 cells. MF1 is purified about 4 fold from RLMC by gel filtration through Sephadex G-50 column. 2. The concentration of MFI that inhibits the growth of L cells to 50% is 270 μg/ml and this specific activity is the highest of the cornin fractions reported up to date. 3. The content of acidic amino acids is more than 23% but that of histidine and sulfurcontaining amino acids is very low in MFl. 4. MFl shows the maximum ultraviolet absorption at 276nm and its main substance is a kind of protein, and its inhibitory effect on the cell growth diminishes by protease treatment. 5. MFl is a single peak both by polyacrylamide gel electrophoresis and by thin layer gel filtration and the molecular weight is calculated about 26,000. 6. MFl is separated in two bands by SDS-polyacrilamide gel electrophoresis and the molecular weight is estimated to be 11,000 and 15,000 respectively. en-copyright= kn-copyright= en-aut-name=DoiAkitaka en-aut-sei=Doi en-aut-mei=Akitaka kn-aut-name=土井昭孚 kn-aut-sei=土井 kn-aut-mei=昭孚 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 END start-ver=1.4 cd-journal=joma no-vol=121 cd-vols= no-issue=3 article-no= start-page=143 end-page=147 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=20091201 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=DNA topoisomerase Uβ activates a subset of neuronal genes kn-title=DNAトポイソメラーゼUβによる神経関連遺伝子の活性化機構 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=SanoKuniaki en-aut-sei=Sano en-aut-mei=Kuniaki kn-aut-name=佐野訓明 kn-aut-sei=佐野 kn-aut-mei=訓明 aut-affil-num=1 ORCID= en-aut-name=MiyajiMary en-aut-sei=Miyaji en-aut-mei=Mary kn-aut-name=宮地まり kn-aut-sei=宮地 kn-aut-mei=まり aut-affil-num=2 ORCID= en-aut-name=TsutsuiM. Kimiko en-aut-sei=Tsutsui en-aut-mei=M. Kimiko kn-aut-name=筒井公子 kn-aut-sei=筒井 kn-aut-mei=公子 aut-affil-num=3 ORCID= en-aut-name=TsutsuiKen en-aut-sei=Tsutsui en-aut-mei=Ken kn-aut-name=筒井研 kn-aut-sei=筒井 kn-aut-mei=研 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　神経ゲノム学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　神経ゲノム学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　神経ゲノム学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科　遺伝情報動態学 en-keyword=DNAトポイソメラーゼUβ　(DNA topoisomeraseUbeta) kn-keyword=DNAトポイソメラーゼUβ　(DNA topoisomeraseUbeta) en-keyword=神経細胞分化 (neuronal differentiation) kn-keyword=神経細胞分化 (neuronal differentiation) en-keyword=遺伝子発現 (gene expression) kn-keyword=遺伝子発現 (gene expression) en-keyword=神経関連遺伝子 (neuronal gene) kn-keyword=神経関連遺伝子 (neuronal gene) en-keyword=クロマチン (chromatin) kn-keyword=クロマチン (chromatin) END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=5-6 article-no= start-page=703 end-page=711 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Detection of circulating immune complexes by the method of conglutinin solid-phase radioimmunoassay I. Experimental study kn-title=Conglutinin Solid-phase Radioimmunoassayによる血中immune complexの検出 第1編 基礎的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Conglutinin solid-phase radioimmunoassay (conglutinin RIA) was utilized for detection of soluble immune complexes in sera. Crude conglutinin was obtained from whole bovine serum using Zymosan as an affinity absorbent. The further purification of crude conglutinin was performed through Sephadex G-200 and DEAE-cellulose column chromatography. The activity of purified conglutinin to haemagglutinate EAC3d was found to be more than 1:2048 in titer. Anti-conglutinin antisera raised in rabbit gave an identical precipitin line against bovine serum and purified conglutinin. Conglutinin RIA was performed using 2.5μg/ml of conglutinin and 25μl of fresh normal human serum as a source of complement. Serum samples were incubated with conglutinin coated on polystylene tubes for 1 hour at room temperature, and incubated with (125)I-labelled anti-human IgG for 4 hours at room temperature. The amounts of immune complexes measurable by this method were in the ranges of 5 to 320μg/ml equivalent to aggregated IgG. Conglutinin RIA was applied to the detection of soluble immune complexes formed in vitro which could be detected both in antigen excess and antibody excess regions. en-copyright= kn-copyright= en-aut-name=TamuraTakahiro en-aut-sei=Tamura en-aut-mei=Takahiro kn-aut-name=田村敬博 kn-aut-sei=田村 kn-aut-mei=敬博 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第3内科教室 en-keyword=immune complex kn-keyword=immune complex en-keyword=conglutinin Radioimmunoassay kn-keyword=conglutinin Radioimmunoassay en-keyword=Clq Radioimmunoassay kn-keyword=Clq Radioimmunoassay en-keyword=免疫複合体 kn-keyword=免疫複合体 END start-ver=1.4 cd-journal=joma no-vol=92 cd-vols= no-issue=3-4 article-no= start-page=407 end-page=418 dt-received= dt-revised= dt-accepted= dt-pub-year=1980 dt-pub=19800430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cell proliferation stimulating factors in the supernatant of embryos and adult muscles of chickens kn-title=鶏胚及び成鶏筋上清中に存在する細胞増殖促進因子について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Factors stimulating cell proliferation were extracted from the supernatant of chick embryo carcases and adult chicken leg muscles of the same strain. Partial purification was done, and the mechanism of stimulation (without supplements) and physicochemical properties were studied. 1). There were at least two or more stimulating factors for cell proliferation of chick embryo fibroblasts in the supernatant of embryo carcases and adult leg muscles of chickens. 2). The embryonic stimulating factors were several times more active than the muscular ones. 3). One of these stimulants was basic and had a low molecular weight protein-like component. 4). One of the partially purified stimulants showed its strongest activity on DNA synthesis in a chick embryo fibroblast system. en-copyright= kn-copyright= en-aut-name=TakahashiFumio en-aut-sei=Takahashi en-aut-mei=Fumio kn-aut-name=高橋史生 kn-aut-sei=高橋 kn-aut-mei=史生 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 en-keyword=鶏胚繊維芽細胞 kn-keyword=鶏胚繊維芽細胞 en-keyword=細胞増殖促進因子 kn-keyword=細胞増殖促進因子 END start-ver=1.4 cd-journal=joma no-vol=94 cd-vols= no-issue=11-12 article-no= start-page=1003 end-page=1008 dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19821230 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Polyacrylamide Gel Electrophoretic Studies of Residual Catalase in Acatalasemia kn-title=無カタラーゼ血症残余カタラーゼのポリアクリルアミド・ゲル電気泳動法による研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Erythrocyte catalase of a Japanese-type acatalasemia case and a normal control subject was separated by chromatofocusing with or without prior partial purification with DEAE cellulose and analyzed by polyacrylamide gradient gel electrophoresis. Two bands of catalase activity were found in the partially purified preparations with molecular weights of 29×10(4) and 35×10(4) in the acatalasemia case and of 28×10(4) and 36×10(4) in the normal control. The molecular weight of normal catalase in untreated hemolysate was 25×10(4). With normal catalase, chromatofocusing fractionation caused a shift toward higher molecular species in the alkaline range eluates from the untreated hemolysate and an opposite shift toward lower molecular weight species in the same pH range eluates from the partially purified preparation. Normal catalase could be identified as protein bands on polyacrylamide gradient gel with hemolysate fractionated by chromatofocusing. Possible application of this technigne in the study of acatalasemia protein with a sensitive protein stain was suggested. en-copyright= kn-copyright= en-aut-name=OgataMasana en-aut-sei=Ogata en-aut-mei=Masana kn-aut-name=緒方正名 kn-aut-sei=緒方 kn-aut-mei=正名 aut-affil-num=1 ORCID= en-aut-name=MizugakiJunko en-aut-sei=Mizugaki en-aut-mei=Junko kn-aut-name=水垣順子 kn-aut-sei=水垣 kn-aut-mei=順子 aut-affil-num=2 ORCID= en-aut-name=IzumiMasaki en-aut-sei=Izumi en-aut-mei=Masaki kn-aut-name=泉正樹 kn-aut-sei=泉 kn-aut-mei=正樹 aut-affil-num=3 ORCID= en-aut-name=TaketaKazuhisa en-aut-sei=Taketa en-aut-mei=Kazuhisa kn-aut-name=武田和久 kn-aut-sei=武田 kn-aut-mei=和久 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生 affil-num=2 en-affil= kn-affil=岡山大学医学部公衆衛生 affil-num=3 en-affil= kn-affil=香川大学保健管理センター affil-num=4 en-affil= kn-affil=香川大学保健管理センター en-keyword=無カタラーゼ血症 kn-keyword=無カタラーゼ血症 en-keyword=ポリアクリルアミド・グラジェント・ゲル電気泳動 kn-keyword=ポリアクリルアミド・グラジェント・ゲル電気泳動 en-keyword=クロマトフォーカシング kn-keyword=クロマトフォーカシング en-keyword=DEAEセルロース・クロマトグラフィー kn-keyword=DEAEセルロース・クロマトグラフィー en-keyword=分子量 kn-keyword=分子量 END start-ver=1.4 cd-journal=joma no-vol=95 cd-vols= no-issue=3-4 article-no= start-page=387 end-page=393 dt-received= dt-revised= dt-accepted= dt-pub-year=1983 dt-pub=19830430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Partial purification and properties of Ca(2+)-binding protein from rat liver mitochondrial matrix. kn-title=ラット肝ミトコンドリア・マトリクスのCa(2+)結合蛋白質の部分精製とその物性について en-subtitle= kn-subtitle= en-abstract= kn-abstract=A 193-fold purification of Ca(2+)-binding protein from rat liver mitochondrial matrix was achieved. The Ca(2+)-binding protein consisted of 3 polypeptide subunits whose respective molecular weights by SDS polyacrylamide gel electrophoresis were 62K, 49K and 37K. The molecular weight of the protein was 150K to 220K. The Kd for Ca(2+) was 1.3×10-(5)M and lower for Mn(2+) and Mg(2+). The protein was inactivated by heat treatment at 100℃ for 1 min, though stable against treatment with 0.5% w/v trypsin at 37℃ for 30 min. Ruthenium red did not inhibit Ca(2+)-binding. en-copyright= kn-copyright= en-aut-name=TokudaMasaaki en-aut-sei=Tokuda en-aut-mei=Masaaki kn-aut-name=徳田雅明 kn-aut-sei=徳田 kn-aut-mei=雅明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一生理学教室 en-keyword=肝ミトコンドリア kn-keyword=肝ミトコンドリア en-keyword=Ca(2+)結合蛋白質 kn-keyword=Ca(2+)結合蛋白質 END start-ver=1.4 cd-journal=joma no-vol=98 cd-vols= no-issue=5-6 article-no= start-page=475 end-page=485 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=19860630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the characteristics of human lung mast cells I. Purification and viability of human lung mast cells kn-title=ヒト肺の肥満細胞に関する研究 第1編 ヒト肺肥満細胞の分離精製に関する検討 en-subtitle= kn-subtitle= en-abstract=In order to clarify the pathophysiology of allergic disorders, it is necessary to study the reactivity of mast cells. Recently, evidence that mast cells have specificities in species and organs has been reported. For use in an investigation of bronchial asthma, I developed a suitable technique for the purification of mast cells using normal lung tissue from patients with lung cancer. The purification steps were: @ dispersion of lung fragments into a single cell suspension with an enzyme mixture (pronase-chymopapain and collagenase-elastase), A centrifugation using a Percoll continuous gradient, and B exclusion of adherent cells. As a result, 2.1±1.1×10(5) mast cells per gram of lung tissue in purities of 38.8±7.2% were obtained. Isolated mast cells proved functionally and morphologically stable by the following findings. The cells showed: @ dye exclusion exceeding 95%, A a histamine content of 4.3±0.6pg/mast cell, B a histamine release of 47.9±11.2% induced by calcium ionophore A23187, and C almost no damage on scanning electron microscopic observation. These results indicate that the present technique for the purification of human lung mast cells is comparatively simple and useful. The isolated mast cells can be applied to pathogenetic and therapeutic studies of bronchial asthma. kn-abstract=19世紀後半にEhrlichによって見出された肥満細胞は,皮膚,肺,腸管をはじめ全身の臓器の結合組織血管周囲に多数分布しており,各種炎症巣において急性炎症期では減少し慢性炎症期では修復機転の続く限り増加するため,炎症反応の初期から修復に至る各時期に関与して外界の環境変化に対応する幅広い役割を担った細胞と考えられている.さらに1966年には石坂らのIgEの発見によってアレルギーの分野における肥満細胞の機能に関する研究が長足の進歩を遂げるに至っている1)〜3).このような肥満細胞の細胞化学,免疫薬理学的な研究においては材料の調整法の理由から主としてラットなど動物由来の肥満細胞が用いられている.また著者らはヒト肥満細胞の代りにヒト末梢血好塩基球を用い,その反応性をアレルギー反応の指標として一連の検討を行なっている4)〜9).しかし,肥満細胞と好塩基球の形態学的あるいは機能的な相違点10)〜14)については以前から指摘されており,また近年種属及び臓器間での肥満細胞のheterogeneityの存在が明らかにされてきた15),16)ことから,気管支喘息をはじめとするアレルギー性肺疾患の病態を解明する上で,各種動物由来の肥満細胞とヒト肺肥満細胞,さらにヒト末梢血好塩基球との相違点を比較検討し,ヒト肺肥満細胞の特異性を明らかにしておく必要があると思われる.以上の目的で従来からヒトの肺小片を用いた肥満細胞の研究17)〜19)が行なわれているが,試薬が組織中の肥満細胞に到達しにくく,かつ遊離された化学伝達物質も正確に定量できないという欠点がある.それを補なうために1972年にGouldらが家兎の肺組織を種々の酵素で処理し単離肥満細胞を分離した20)のに始まり,ヒト肺では1976年にAustenらが同様の方法で分離を試み21),1982年Lichtensteinらはその方法を発展させて高率のヒト肺単離肥満細胞を得ることに成功している22),しかしながら以上の方法では細胞の回収率や繁雑な精製手段などの問題点が残されている.そこで著者はヒト肺肥満細胞の機能を検討する手段として,外科的切除肺から肥満細胞を分離精製する方法とviabilityについて種々検討し,細胞障害性が少なく回収率が良く,かつ比較的簡便な分離法を考案したので報告する. en-copyright= kn-copyright= en-aut-name=MaedaMasanori en-aut-sei=Maeda en-aut-mei=Masanori kn-aut-name=前田昌則 kn-aut-sei=前田 kn-aut-mei=昌則 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=human lung mast cell kn-keyword=human lung mast cell en-keyword=purification kn-keyword=purification en-keyword=viability kn-keyword=viability en-keyword=microscopic observation kn-keyword=microscopic observation en-keyword=histamine kn-keyword=histamine END start-ver=1.4 cd-journal=joma no-vol=98 cd-vols= no-issue=5-6 article-no= start-page=457 end-page=464 dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=19860630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Helix pomatia A hemagglutinin binding activity to peripheral T-lymphocytes in malignant digestive tract diseases Part 1: Assay method based on (125)I-labelled Helix pomatia A hemagglutinin binding to peripheral lymphocytes kn-title=消化器癌患者の末梢血T cellのHelix pomatia A hemagglutinin 結合活性の検討 第1編 (125)I標識Helix pomatia A hemagglutinin のリンパ球への結合活性測定の基礎的検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=For studing aberrant cellular immunity in malignant digestive tract diseases, Helix pomatia A hemagglutinin (HP) binding activity, a T cell surface marker, to peripheral lymphocytes was studied in patients with digestive tract cancer. In this paper, basic experiments on an assay method based on (125)I-labelled HP binding to neuraminidase-treated peripheral lymphocytes are described. HP was labelled with (125)I using the chlormine-T method, and the purif-ication of (125)I-labelled HP was achieved by agarose-aminocaproyl-D-galactosamine affinity chromatography. The results show that (125)I-labelled HP could specifically bind to neuraminidase-treated lymphocytes as cold HP. The optimum assay condition was determined to be: 1×10(6) neuraminidase-treated peripheral lymphocytes incubated with 0.4μg (125)I-labelled HP in 0.5 ml of reaction mixture at 25℃ for 90 min with gentle shaking. In addition, the effect of treatment of peripheral lymphocytes with neuraminidase on E-rosette formation was studied. Enhancement of E-rosette formation by neuraminidase-treatment was not statistically significant. en-copyright= kn-copyright= en-aut-name=YasuharaTakashi en-aut-sei=Yasuhara en-aut-mei=Takashi kn-aut-name=安原高士 kn-aut-sei=安原 kn-aut-mei=高士 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第1内科教室 en-keyword=Helix pomatia A hemagglutinin kn-keyword=Helix pomatia A hemagglutinin en-keyword=結合活性 kn-keyword=結合活性 en-keyword=消化器癌 kn-keyword=消化器癌 en-keyword=末梢血リンパ球 kn-keyword=末梢血リンパ球 en-keyword=neuraminidase kn-keyword=neuraminidase END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=5-6 article-no= start-page=419 end-page=428 dt-received= dt-revised= dt-accepted= dt-pub-year=1985 dt-pub=19850630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Molecular cloning of the SV40 small t antigen gene and purification of the antigen kn-title=SV40小型腫瘍抗原遺伝子のクローン化と抗原の精製 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The simian virus 40 (SV40) DNA fragment encoding small t antigen was cloned with expression vector pUC8 for the purification of small t-antigen. Small t antigen produced in Escherichia coli was a hybrid protein of 22,000 daltons which comprised about 6% of the total protein. Hybrid small t antigen maintained antigenic activity which was examined by Western-blotting and enzyme-linked immunoassay. Hybrid small t antigen was extracted from E. coli with urea and purified from a small t-antigen band in SDS-polyacrylamide gel. The purified antigen maintained antigenic activity. en-copyright= kn-copyright= en-aut-name=IkedaShogo en-aut-sei=Ikeda en-aut-mei=Shogo kn-aut-name=池田正五 kn-aut-sei=池田 kn-aut-mei=正五 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部癌源研究施設生化学部門 en-keyword=SV40 t 抗原 kn-keyword=SV40 t 抗原 en-keyword=遺伝子のクローン化 kn-keyword=遺伝子のクローン化 en-keyword=発現ベクター kn-keyword=発現ベクター en-keyword=ウエスタン・ブロッティング kn-keyword=ウエスタン・ブロッティング END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=9-10 article-no= start-page=1257 end-page=1267 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19871031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on (45)Ca uptake and histamine release by rat peritoneal mast cells Part I Purification and reactivity of rat peritoneal mast cells kn-title=ラット腹腔肥満細胞の(45)Ca uptakeおよびヒスタミン遊離に関する研究　第1編　ラット腹腔肥満細胞の分離法並びに反応性の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Purification of mast cells from the peritoneal cavity of rats and reactivity of the cells to antigen were studies. After peritoneal lavage, the peritoneal cells were washed, the mast cells were isolated by a density gradient centrifugation method. The recovery rate of mast cells was highest when mast cells were separated by BSA density gradient centrifugation after washing the cells on time by low speed centrifugation (50xg). The purity of mast cells obtained by this method was 94.1±1.0%, and the number of mast cells obtained was approximately 1.46×10(6)　cells/rat. Mast cells of actively sensitized rats showed a significant increase in (45)Ca uptake and histamine release after stimulation by antigen. (45)Ca uptake by mast cells of passively sensitized rats was affected by BSA medium, and the most suitable results were obtained using mast cells passively sensitized before separation of the cells by BSA. Mast cells showed a significant increase in (45)Ca uptake after stimulation by various stimulatory agents, such as Con. A, Comp. 48/80 and Ca ionophore A 23187. These results indicate that rat peritoneal mast cells isolated by the present method can be used in studies on Ca(2+) influx into the cells. en-copyright= kn-copyright= en-aut-name=OhtaniJun en-aut-sei=Ohtani en-aut-mei=Jun kn-aut-name=大谷純 kn-aut-sei=大谷 kn-aut-mei=純 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第2内科学教室 en-keyword=rat peritoneal mast call kn-keyword=rat peritoneal mast call en-keyword=purification kn-keyword=purification en-keyword=reactivity kn-keyword=reactivity en-keyword=(45)Ca uptake kn-keyword=(45)Ca uptake en-keyword=histamine release kn-keyword=histamine release END start-ver=1.4 cd-journal=joma no-vol=100 cd-vols= no-issue=5-6 article-no= start-page=473 end-page=482 dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=1988 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Determination of phenylacetic acid in the rat brain by gas chromatography/negative ion chemical ionization mass spectrometry kn-title=ガスクロマトグラフィー／陰イオン化学イオン化質量分析法によるラット脳内フェニル酢酸の定量 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A method coupling gas chromatography/negative ion chemical ionization mass spectrometry (GC/NCI/MS) with a rapid one-step purification on a Sephadex G-10 column was developed to measure free phenylacetic acid(PAA), the major metabolite of ,β-phenylethylamine (PEA), in small amounts of the rat brain. Recovery of PAA for the Sephadex G-10 column was 96.2±2.0%.Pentafluorobenzyl derivatives of PAA (PAA-PFB) and heptadeuterated PAA (PAA-d(7)-PFB) were determined by GC/NCI/MS. No interfering peaks were present on the mass fragmentogram. A calibration curve constructed from the observed peak area ratio of PAA-PFB to PAA-d(7)-PFB and known weight ratio was linear within the range of 1.25 to 40pg PAA, with a correlation coefficient of more than 0.999. In the rat striatum, PAA was determined with 'within batch' and 'between batches' coefficients of variation of 0.81% and 1.24%, respectively.While PAA was significantly decreased by 40% of controls following treatment with pargyline,the monoamine oxidase inhibitor, a remarkable increase in the metabolite level was found following treatment with PEA. Acute and chronic treatment with antidepressants had no significant effects on PAA levels in the rat striatum. The present method should be useful in future investigations of the functional role of PEA in the brain. en-copyright= kn-copyright= en-aut-name=KawabataMasahiro en-aut-sei=Kawabata en-aut-mei=Masahiro kn-aut-name=川端昌弘 kn-aut-sei=川端 kn-aut-mei=昌弘 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部脳代謝研究施設病態生化学部門 en-keyword=フェニル酢酸 kn-keyword=フェニル酢酸 en-keyword=ガスクロマトグラフィー／陰イオン化学イオン化質量分析法 kn-keyword=ガスクロマトグラフィー／陰イオン化学イオン化質量分析法 en-keyword=セファデックスG-10 kn-keyword=セファデックスG-10 en-keyword=抗うつ薬 kn-keyword=抗うつ薬 en-keyword=βーフェニルエチルアミン kn-keyword=βーフェニルエチルアミン END start-ver=1.4 cd-journal=joma no-vol=99 cd-vols= no-issue=3-4 article-no= start-page=179 end-page=189 dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19870430 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Human biliary mucin : purification, characterization, and its serum levels in liver diseases kn-title=ヒト胆汁ムチンの精製,性状および肝疾患における血中濃度 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Human biliary mucin was isolated using Sepharose 6B gel chromatography and equilibrium density gradient centrifugation in caesium chloride. The carbohydrate and amino acid composition of mucin from patients with cholesterol gallstones was essentially the same as that from patients with pigmented gallstones. The molar ratio of galactosamine : glucosamine : galactose : fucose : sialic acid was 1:3:2~4:3~5:0.5. Serine, threonine, and glycine were major amino acids. Anti-mucin antibody was raised in a rabbit, and was found to be specific for human biliary mucin. Serum levels of antibody-reactive proteins were significantly elevated in patients with acute and chronic liver diseases, and patients with gallstones. They were significantly higher in chronic parenchymal liver disease patients than in acute hepatitis patients, and in liver cirrhosis and hepatocellular carcinoma patients than in chronic hepatitis patients. The levels in gallstone patients were comparable to those in chronic hepatitis patients. The present results suggest that 1) the carbohydrate composition of mucin dose not differ between cholesterol gallstones and pigmented gallstones, 2) serum mucin, which reacts with the antibody against biliary mucin, is increased in liver disease patients, and this mucin might originate from the biliary tract, and 3) among liver disease patients, those with liver cirrhosis and hepatocellular carcinoma have the highest serum mucin. en-copyright= kn-copyright= en-aut-name=TorigoeShouichiro en-aut-sei=Torigoe en-aut-mei=Shouichiro kn-aut-name=鳥越昇一郎 kn-aut-sei=鳥越 kn-aut-mei=昇一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一内科教室 en-keyword=胆嚢胆汁 kn-keyword=胆嚢胆汁 en-keyword=ヒト胆汁ムチン kn-keyword=ヒト胆汁ムチン en-keyword=化学的組成 kn-keyword=化学的組成 en-keyword=血中濃度 kn-keyword=血中濃度 en-keyword=肝疾患 kn-keyword=肝疾患 END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=2 article-no= start-page=105 end-page=111 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990226 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒスチジンタグを持つホスファカンコア蛋白の大腸菌での発現と精製 kn-title=Affinity purification of phosphacan core protein expressed in Escherichia coli as histidine-tagged fusion protein en-subtitle= kn-subtitle= en-abstract=コンドロイチン硫酸プロテオグリカンの一つであるホスファカンのコア蛋白の特定領域を,ヒスチジンタグ(His-tag)を持つ融合蛋白として大腸菌内で発現させニッケル-ニトリロ3酢酸(Ni-NTA)アフィニティ担体を用いて精製した｡ホスファカンコア蛋白のアミノ酸残基343-446(P3)及び1-340(P4)に相当するcDNA断片を,胎性18日目のラット脳由来のmRNAを鋳型としたPCRによって増幅した｡増幅された断片は発現ベクターpQE30に組み込まれ,これで大腸菌(M15[pREP4])を形質転換した｡His-tag融合蛋白の発現は形質転換株を1mM IPTG存在下で37℃,5時間培養することによって行われた｡His-tagged P3融合蛋白は可溶性蛋白質として発現し,Ni-NTA担体を用いて精製された.His-tagged P4融合蛋白は不溶性の封入体を形成したが,8M尿素によって可溶化され,変性条件下で同様に精製された｡ kn-abstract=Specific regions of core protein of phosphacan, one of the chondroitin sulfate proteoglycans, were expressed as fusion proteins with histidine-tag (His-tag) in Escherichia coli (E.coli) and were affinity purified using nickel-nitrilotriacetic acid (Ni-NTA) matrix. cDNA fragments encoding amino acid residues 343-446 (P3) and 1-340 (P4) of phosphacan core protein were amplified by polymerase chain reaction from E18 rat brain mRNA as template. The amplified products were subcloned into pQE30 vector and were introduced into E.coli strain M15 [pREP4] for the expression. The His-tagged fusion proteins were expressed by cultivating the transformants at 37℃ for 5h in the presence of 1mM IPTG. His-tagged P3 fusion protein (His-P3) was expressed as soluble form, and was purified using Ni-NTA matrix. His-tagged P4 fusion protein (His-P4) which was sequestered into insoluble inclusion bodies was treated with 8.0M urea to solubilize, and then was purified under denaturing conditions. en-copyright= kn-copyright= en-aut-name=ItoSekiko en-aut-sei=Ito en-aut-mei=Sekiko kn-aut-name=伊藤昔子 kn-aut-sei=伊藤 kn-aut-mei=昔子 aut-affil-num=1 ORCID= en-aut-name=OkamotoMotoi en-aut-sei=Okamoto en-aut-mei=Motoi kn-aut-name=岡本基 kn-aut-sei=岡本 kn-aut-mei=基 aut-affil-num=2 ORCID= en-aut-name=MoriShuji en-aut-sei=Mori en-aut-mei=Shuji kn-aut-name=森秀治 kn-aut-sei=森 kn-aut-mei=秀治 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=2 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 affil-num=3 en-affil= kn-affil=岡山大学医学部保健学科検査技術科学専攻 en-keyword=phosphacan (ホスファカン) kn-keyword=phosphacan (ホスファカン) en-keyword=core protein (コア蛋白) kn-keyword=core protein (コア蛋白) en-keyword=His-tagged proteins kn-keyword=His-tagged proteins en-keyword=recombinant protein (融合蛋白) kn-keyword=recombinant protein (融合蛋白) END start-ver=1.4 cd-journal=joma no-vol=102 cd-vols= no-issue=1-2 article-no= start-page=199 end-page=207 dt-received= dt-revised= dt-accepted= dt-pub-year=1990 dt-pub=199002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Structural protein analysis of intracisternal A particles in adenovirus-induced mouse tumor kn-title=内在性小胞体内 A 粒子の構成蛋白に関する研究ーアデノウイルス12型誘発マウス腫瘍に認められた粒子についてー en-subtitle= kn-subtitle= en-abstract= kn-abstract=Analysis and purfication of structural proteins of intracisternal A particles produced in adenovirus-induced tumor were described. SDS-PAGE of purified intracisternal A particles demonstrated its major structural components, K92, K70 and K55, and minor components, K43 and K37. Two dimensional gel electrophoresis indicated a pI of K70 and K55 at 6.5 and 6.3, respectively, in the presence of sodium dodecyl sulfate. Purification of the main band, K70, in SDS-PAGE using reversed phase-HPLC was difficult in the standard acidic condition, but could be achieved in the neuttral condition. Although purificatin of K70 is generally difficult because of its hydrophobicity, the method shown here will be useful for furthey study of structural proteins of intracisternal A particles. en-copyright= kn-copyright= en-aut-name=HirakawaEiichiro en-aut-sei=Hirakawa en-aut-mei=Eiichiro kn-aut-name=平川栄一郎 kn-aut-sei=平川 kn-aut-mei=栄一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=香川医科大学病理学講座第一病理学 en-keyword=小胞体内 A 粒子 kn-keyword=小胞体内 A 粒子 en-keyword=アデノウイルス誘発腫瘍 kn-keyword=アデノウイルス誘発腫瘍 en-keyword=構成蛋白分析 kn-keyword=構成蛋白分析 en-keyword=reversed phase-HPLC kn-keyword=reversed phase-HPLC END start-ver=1.4 cd-journal=joma no-vol=78 cd-vols= no-issue=6 article-no= start-page=683 end-page=694 dt-received= dt-revised= dt-accepted= dt-pub-year=1966 dt-pub=19660630 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=螢光抗体法によるマウス白血病ウイルスの研究 第1編 C58マウス白血病ウイルス抗原の精製-特にFluorocarbon処理法の検討- en-subtitle= kn-subtitle= en-abstract= kn-abstract=Purification of viruses of C58 mouse leukemias was carried out by the following procedures; filtration, differential ultracentrifugation and Gessler's fluorocarbon extraction. The degree of virus purification was examined by fluorescent antibody method, electron microscopy, nucleic acid analysis and bioassay. The following results were obtained. 1. Large amount of host cell antigens was noted in virus suspension separated by Berkefeld-N and Chamberland L3 filtration and by differential ultracentrifugation. Therefore it was improper to perform immunofluorescent study because this procedure was concerned with examination of viral antigens. 2. Immunofluorescent procedure by Coons' direct method using anti-viral serum against fluarocarbon extracts showed partially granular or partially diffuse specific fluorescence in leukemic cells and intercellular spaces of the leukemic organs. 3. Electron microscopic observation revealed many virus-like particles in the fluorocarbon extracts. And 50% of animals inoculated with developed leukemias in these extracts. 4. Ultraviolet spectrophotometry of the fluorocarbon extracts demonstrated a maximal absorption at 260 mμ and a minimal absorption at 230 mμ with a Bechman spectrophotometer. It was clear from these data that large amount of nucleic acid was contained in these extracts. And also it was indicated by Bial's test that the fluorocarbon extracts of the leukemia organs contained large amount of RNA. 5. From above findings, fluorocarbon extraction appeared a superior procedure for fluorescent antibody technic in comparison with the other procedurs used. It was thought that the specific fluorescence in the immunofluorescent study represented viral antigen itself. en-copyright= kn-copyright= en-aut-name=TakahashiYoshiaki en-aut-sei=Takahashi en-aut-mei=Yoshiaki kn-aut-name=高橋喜亮 kn-aut-sei=高橋 kn-aut-mei=喜亮 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部平木内科 END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=4 article-no= start-page=419 end-page=434 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=1991 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Clinical studies on serum factors in patients with bronchal asthma Part 1. Changes in α(1)-Antitrypsin and α(1)-Acid glycoprotein levels in patients with bronchial asthma and other respiratory diseases kn-title=気管支喘息における体液因子に関する研究 第1編 血清糖蛋白 (α(1)-Antitrypsin, α(1)-Acid glycoprotein) の変動について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Serum α(1)-Antitrypsin (α(1)-AT) and α(1)-Acid glycoprotein (α(1)-AG) levels were measured in 34 healthy subjects, 167 patients with bronchial asthma and 82 cases with other respiratory diseases. The serum levels of α(1)-AT were significantly increased in patients over 40 years old and the levels of α(1)-AG were significantly higher in patients over 50 years old compared to the those under 50 years old. The serum levels of α(1)-AT and α(1)-AG were significantly more increased in asthmatic patients with mixed and infective type during asymptomatic periods than in healthy subjects. The levels of α(1)-AT were significantly higher in patients with mild and moderate asthma than in healthy subjects. The levels of α(1)-AG were also significantly higher in mild and severe asthmatic patients than in healthy subjects. Patients with severe asthma showed more increased levels of α(1)-AT during attack periods compared to that during non-attack periods. The levels of α(1)-AT and α(1)-AG in asthma patients with steroid therapy were within normal limits during the non-attack periods, but significantly increased during the attack periods. In asthmatic patients with mixed type, infectious type, severe type and steroid therapy, a correlation was observed between the serum levels of α(1)-AT and α(1)-AG during their attack periods. en-copyright= kn-copyright= en-aut-name=IshibashiKen en-aut-sei=Ishibashi en-aut-mei=Ken kn-aut-name=石橋健 kn-aut-sei=石橋 kn-aut-mei=健 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=気管支喘息 kn-keyword=気管支喘息 en-keyword=α(1)-Antitrypsin kn-keyword=α(1)-Antitrypsin en-keyword=α(1)-Acid glycoprotein kn-keyword=α(1)-Acid glycoprotein END start-ver=1.4 cd-journal=joma no-vol=98 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=2009 dt-pub=200902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Expression, Purification and Properties of Alanine Racemase from Thermus thermophillus HB8 kn-title=高度好熱性細菌 Thermus thermophilus HB8 由来アラニンラセマーゼの大腸菌での発現，精製及び諸性質の検討 en-subtitle= kn-subtitle= en-abstract=高度好熱性細菌 Thermus thermophilus HB8 由来アラニンラセマーゼ遺伝子を大腸菌中にクローニングし、発現させた後に、精製及び性質検討を行った。alr遺伝子は1080bpからなり360アミノ酸残基 HB8をコードしていたので、本酵素は38,596Daの分子量であると予想された。alr遺伝子の [ G + C ] 含量は、72%であり、Tm値は98.8℃であった。T.thermophilus HB8由来アラニンセマーゼを中等度好熱性細菌 Geobacillus stearothermophilus 及び赤痢菌 Shigella sonnei 由来アラニンラセマーゼと一次配列の比較をしたところ、G.stearothermophilus 由来のものと33%、赤痢菌由来の酵素を28%の相同性を示した。T.thermophilus HB8 由来アラニンラセマーゼを、70℃で10分間の熱処理後、DEAE-トーヨーパール陰イオン交換カラム等により精製した。精製酵素の最適温度は、d-アラニンからl-アラニンへの反応で55℃、l-アラニンからd-アラニンへの反応では60℃であり、最適pHは、9.0?10.0であった。また、70℃で30分インキュベーションを行った後にも、活性の低下は見受けられず耐熱性を示した。更に、本酵素は分子量38,000モノマー酵素であると推定され、その反応機構に興味が持たれる。 kn-abstract=An alanine racemase (EC 5.1.1.1) from an extreme thermophilic bacterium Thermus thermophilus HB8, was purified and characterized, and its gene was cloned. The cloned alanine racemase gene (alr) was expressed in Escherichia coli JM 109. The alr gene is composed of a 1080 bp and encoded a 360 amino acid, and was predicted to have a molecular weight of 38,596. The enzyme was purified by heat shock at 70°C for 10min and DEAE Toyopearl 650M column chromatography. The purified enzyme had an optimum pH9.0?10.0 and an optimum temperature of 55°C?60°C. Enzyme activity was retained 100% after incubation of the enzyme at 70°C for 10min. Alanine racemase from Thermus thermophilus HB8 is a monomeric enzyme with a molecular mass of 39 kDa. en-copyright= kn-copyright= en-aut-name=YanagitaniMasahiko en-aut-sei=Yanagitani en-aut-mei=Masahiko kn-aut-name=柳谷昌彦 kn-aut-sei=柳谷 kn-aut-mei=昌彦 aut-affil-num=1 ORCID= en-aut-name=UemaeSatoshi en-aut-sei=Uemae en-aut-mei=Satoshi kn-aut-name=上前智 kn-aut-sei=上前 kn-aut-mei=智 aut-affil-num=2 ORCID= en-aut-name=ShiragaTomoyuki en-aut-sei=Shiraga en-aut-mei=Tomoyuki kn-aut-name=白神智行 kn-aut-sei=白神 kn-aut-mei=智行 aut-affil-num=3 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=4 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=alanine racemase kn-keyword=alanine racemase en-keyword=pyridoxal 5’-phosphate kn-keyword=pyridoxal 5’-phosphate en-keyword=thermostable enzyme kn-keyword=thermostable enzyme en-keyword=Thermus thermophilus HB8 kn-keyword=Thermus thermophilus HB8 END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=5-6 article-no= start-page=471 end-page=482 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effects of typtophan metabolites on brain function : Electrocorticographical study kn-title=トリプトファン代謝産物のラット脳機能に対する影響の研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The effects of tryptophan (Trp) metabolites administered into right cerebroventricle (1μmol) on the electrocorticograms (ECoG) of rats were studied to investigate the roles of Trp metabolites in the brain function. Kynurenine, anthranilic acid, and xanthurenic acid has no effect on ECoG until the end of recording 4 hours after the administration. 3-Hydroxykynurenine had a suppressive effect on the ECoG transitory, and kynurenic acid suppressed ECoG slightly. 3-Hydroxyanthranilic acid which is a metabolite of 3-hydroxykynurenine, induced spike discharges with a long latency (60-230 min after the administration). 3-Hydroxyanthranilic acid is thought to be metabolized to o-aminophenol, quinolinic acid and picolinic acid. Among the 3-hydroxyanthranilic acid metabolites, o-aminophenol induced spike discharges a few min after the administration, and the spike discharges a few min after the administrations, and the spike discharges lasted 60 min. On the other hand, quinolinic acid suppressed ECoG, and picolinic acid had no effect. These electrocorticographic findings suggest that 3- hydroxyanthranilc acid might induce spike discharges after metabolization to o-aminophenol. en-copyright= kn-copyright= en-aut-name=NishijimaYutaka en-aut-sei=Nishijima en-aut-mei=Yutaka kn-aut-name=西嶋寛 kn-aut-sei=西嶋 kn-aut-mei=寛 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学歯学部口腔外科学第一講座 en-keyword=kynurenic acid kn-keyword=kynurenic acid en-keyword=3-hydroxyanthranilic acid kn-keyword=3-hydroxyanthranilic acid en-keyword=o-aminophenol kn-keyword=o-aminophenol en-keyword=quinolinic acid kn-keyword=quinolinic acid en-keyword=experimental seizures kn-keyword=experimental seizures END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=3-4 article-no= start-page=323 end-page=330 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=1,25-Dihydroxyvitamin D(3) stimulates the secretion of insulin-like growth factor binding protein 3 (IGFBF-3) by cultured human osteosarcoma cells kn-title=1,25-DihydroxyvitaminD(3)によるヒト骨肉腫細胞 MG63 からのinsulin-like growth factor binding protein 3 の産生増加 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Several types specific insulin-like growth factor binding proteins (IGFBPs) are produced by peripheral tissue-derived cells and they modulate the functions of insulin-like growth factors. In this study, both the secretion of IGFBP-3 from a human osteosarcoma cell line MG63 and effects of 1, 25-dihydroxyvitamin D(3) (1,25-(OH)(2)D(3)) on the production of IGFBP-3 were investigated. The β subunit of IGFBP-3 was detected immunocytochemically in the perinuclear cytoplasm of MG63 cells. Immunoblotting and SDS-PAGE analysis revealed that both 140-150KD MW entire molecules and 40-60KD MW β subinit molecules of IGFBP-3 were present in cell-conditionel media. 1,25-(OH)(2)D(3) stimulated the production of the IGFBP-3 molecule by MG63 cells. The concentration of IGFBP-3 conditioned media began to rise at 24 hours after the addtiton of 10(-9)M of 1,25-(OH)(2)D(3) and reached the peak level at 48 hours. Dose-dependent effects of 1,25-(OH)(2)D(3) were demonstrated. These findings show that MG63 produces IGFBP-3 and that 1,25-(OH)(2)D(3) stimulates the production of this protein. These findings suggest that the synergistic effects of 1,25-(OH)(2)D(3) on the action of IGF-I on osteoblastic cells may be modulated by locally produced IGFBP-3. en-copyright= kn-copyright= en-aut-name=MoriwakeTadashi en-aut-sei=Moriwake en-aut-mei=Tadashi kn-aut-name=守分正 kn-aut-sei=守分 kn-aut-mei=正 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=1,25 (OH)(2)D(3) kn-keyword=1,25 (OH)(2)D(3) en-keyword=insulin-like growth factor binding protein 3 kn-keyword=insulin-like growth factor binding protein 3 en-keyword=human osteosarcoma cell line MG63 kn-keyword=human osteosarcoma cell line MG63 END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=11-12 article-no= start-page=1135 end-page=1144 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=199212 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Examination of ANP secretion-atrial specific granules and hemodynamics kn-title=ANP 分泌―血行動態と特殊顆粒に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify factors affecting atrial natriuretic peptide (ANP) secretion, the relationship between blood ANP level and intracardiac pressure, and that between the number of atrial specific granules (ASG) in the right atrial auricle and ANP level were investigated in 21 patients undergoing extracorporeal circulation. In the atrial loaded group, the Group TR (Tricuspid regurgitation) served as right atrial load and Group M (Mitral valve disease) as left atrial load, ANP and the number of ASG were compared with those in the control group. Accordingly, the effect of atrial load on ANP secretion and the number of ASG were investigated. Moreover, ANP and the number of ASG in the atrial myolysis group {Group A f(atrial fibrillation)} were compared with those of the sinus rhythm group to determine effect of atrio-myolysis. The ANP level showed a positive correlation with left atrial pressure (r=0.820 p<0.01) and with left ventricular end-diastolic pressure (r=0.726 p<0.01), but no correlation with right atrial pressure nor ventricular pressure. The ANP level in Group M was significantly higher than other groups, but there was no significant difference compared with Group TR. Consequently, the left atrium and rihgt atrium were considered to have different effects on ANP secretion. en-copyright= kn-copyright= en-aut-name=AsanoHirotaka en-aut-sei=Asano en-aut-mei=Hirotaka kn-aut-name=浅野弘孝 kn-aut-sei=浅野 kn-aut-mei=弘孝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二外科学教室 en-keyword=特殊顆粒 kn-keyword=特殊顆粒 en-keyword=ANP kn-keyword=ANP en-keyword=分泌調節 kn-keyword=分泌調節 en-keyword=左心房 kn-keyword=左心房 en-keyword=右心房 kn-keyword=右心房 END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=7-8 article-no= start-page=879 end-page=888 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Regulation of transferrin receptor (TfR) expression on erythroblasts from patients with myelodysplastic syndrome Part 2. Analysis of TfR expression using stainable iron granules in myelodysplastic syndrome kn-title=骨髄異形成症候群におけるヒト赤芽球トランスフェリン受容体発現に関する研究　第2編　骨髄異形成症候群におけるトランスフェリン受容体発現の特徴について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Expression of TfR on the cell surface is known to be regulated by the cellular iron content. We previously reported an inverse correlation between mean number of TfR expressed and stainable iron granules (SIGs) of erythroblasts (EBLs) in normal subjects. In MDS, EBLs contain an increased number of SIGs, though the mechanism of this phenomenon is not well understood. In this report, expression of surface transferrin receptor (TfR) on bone marrow EBLs was examined in comparison with the number of SIGs in 11 patients with myelodysplastic syndrome (MDS) and 11 patients with acute leukemia (AL). The mean numbers of TfR from MDS (5.56±1.35x10(5)) and AL (5.73±2.25x10(5)) were not different from those of normal subjects (5.00±1.80x10(5)). However, in MDS the mean number of TfR from 8 patients (72%) was similar to the normal level in spite of the increased mean number of SIG. Four of the 11 patients with AL showed a similar change such as MDS in TfR, but notably 2 of those 4 patients were AL transformed from MDS. There is a correlation (r＝0.61) between TfR and MCV in MDS different from that in healthy subject. These findings suggest that TfR expression on EBLs, from MDS may escape from the regulation by the cellular iron content as iron-TfR regulation disturbance. en-copyright= kn-copyright= en-aut-name=TsuyunoRitsurou en-aut-sei=Tsuyuno en-aut-mei=Ritsurou kn-aut-name=露野理津朗 kn-aut-sei=露野 kn-aut-mei=理津朗 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=transferrin receptor kn-keyword=transferrin receptor en-keyword=stainable iron granule kn-keyword=stainable iron granule en-keyword=myelodysplastic syndrome kn-keyword=myelodysplastic syndrome en-keyword=acute leukemia kn-keyword=acute leukemia END start-ver=1.4 cd-journal=joma no-vol=104 cd-vols= no-issue=1-2 article-no= start-page=75 end-page=82 dt-received= dt-revised= dt-accepted= dt-pub-year=1992 dt-pub=1992 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of immunosuppressive substance against the potency of natural human tumor necrosis factor as biological response modifier kn-title=Biological response modifier としての Tumor necrosis fator の効果に対する Immunosuppressive substance の作用の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=TNF is a cytokine with the activity of a BRM (biological response modifier). TNF-α and TNF-β enhanced NK activity of peripheral blood mononucleocytes of a normal donor, but not in a cancer patient. ISS, a glocoprotein extracted from the ascitic fluid of a colon cancer patient with immunosuppressive properties, ia also detected in large quantities in the serum of cancer patients. NK activity of a normal donor which was in an immunosuppressive state by the administration of ISS was not affected by treatment of TNF-α or TNF-β, but the suppressed NK activity was improved by the combination of TNF with IFN-α or IFN-γ. On the other hand, NK activity of a cancer patient treated with anti-IS antiserum which was obtained from serum of rabbit immunized by ISS was enhanced by both TNFs. These findings suggest that ISS suppresses the effect of TNFs on NK activity. Furthermore, the effect of TNF as a BRM is inhibited in cancer patients due to the high dose of ISS in the serum, and that the combination of TNF with other cytokines, such as IFN, is more effective than the single use of TNF, clinically. en-copyright= kn-copyright= en-aut-name=YasuiYoshimasa en-aut-sei=Yasui en-aut-mei=Yoshimasa kn-aut-name=安井義政 kn-aut-sei=安井 kn-aut-mei=義政 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第一外科学教室 en-keyword=biological response modifier (BRM) kn-keyword=biological response modifier (BRM) en-keyword=tumor necrosis fator (TNF) kn-keyword=tumor necrosis fator (TNF) en-keyword=immunosuppressive substance (IS 物質) kn-keyword=immunosuppressive substance (IS 物質) en-keyword=natural killer (NK) 活性 kn-keyword=natural killer (NK) 活性 END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=7-8 article-no= start-page=791 end-page=801 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the regulation of eosinophils in bronchial asthma Part 2. Migratory responses of eosinophils from asthmatics kn-title=気管支喘息における好酸球動態の調節に関する研究　第2編　喘息患者末梢血好酸球の遊走能に関する検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eosinophil infiltration in lung tissue is one of the characteristic features of bronchial asthma. Such cell infiltration seems to be induced by the eosinophil chemotactic factor (ECF). PAF and IL-5 are potent chemoattractants and activators for eosinophils. To evaluate the reactivity of eosinophils in asthmatics under various conditions, the migratory function of eosinophils to PAF and IL-5 was investigated by the modified Boyden chamber method. Eosinophils of asthmatics were highly purified using a flow cytometric method previously reported. The migratory response of the eosinophils of asthmatics was greater than that of healthy suljects. Eosinophils from atopic asthmatics showed a higher response to PAF than those from non-atopic asthmatics. Eosinophils in the attack stage showed a higher response than those in the non-attack stage. Hypodense eosinophils showed an increased migratory response. The migratory response was correlated to the serum concentration of ECP and blood eosinophil count. These findings suggest that the reactivity of eosinophils is heterogenous and relates to the degree of eosinophilia, and that IL-5 as well as PAF plays an important role in the pathogenesis of bronchial asthma. en-copyright= kn-copyright= en-aut-name=TakahashiHisaho en-aut-sei=Takahashi en-aut-mei=Hisaho kn-aut-name=高橋寿保 kn-aut-sei=高橋 kn-aut-mei=寿保 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Bronchial asthma kn-keyword=Bronchial asthma en-keyword=Eosinophil migratory response kn-keyword=Eosinophil migratory response en-keyword=Platelet-activating factor kn-keyword=Platelet-activating factor en-keyword=Interleukin-5 kn-keyword=Interleukin-5 en-keyword=Eosinophil heterogeneity kn-keyword=Eosinophil heterogeneity END start-ver=1.4 cd-journal=joma no-vol=103 cd-vols= no-issue=7-8 article-no= start-page=779 end-page=789 dt-received= dt-revised= dt-accepted= dt-pub-year=1991 dt-pub=199108 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on the regulation of eosinophils in bronchial asthma Part 1. Characterization of eosinophil chemotactic factor (ECF) derived from peripheral blood mononuclear cells stimulated with Candida antigen kn-title=気管支喘息における好酸球動態の調節に関する研究　第1編　喘息患者末梢血単核球由来の好酸球遊走因子（ECF）の解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eosinophilic infiltration in bronchial tissue is characteristic in the pathogenesis of bronchial asthma. The eosinophil chemotactic factor (ECF) derived from mononuclear cells has been reported to have some effect on the cell infiltration, and interleukin-5 (IL-5), a lymphokine derived from T lymphocytes, to be a factor related to growth, chemotaxis and activation for eosinophils. Lymphocytes accumulated in the bronchoalveolar lavage fluids of non-atopic and severe asthmatics have been shown to be highly responsive to Candida antigen, and high ECF production was observed in non-atopic and severe asthmatics by measurement of ECF activity in the supernatant of peripheral blood mononuclear cells cultured with Candida antigen. In this report, the molecular weight by gel filtration and inhibition test using anti-murine IL-5 antibody were studied to characterize the lymphocyte-derived ECF. Gel filtration analysis of the ECF indicated a molecular weight of 20,000 to 65,000 Da with a peak of activity around 40,000 to 50,000 Da. The ECF activity was reduced by incubation with anti-murine IL-5 antibody, which suggests that the supernatant contains IL-5. ECF from mononuclear cells, containing IL-5, may play an important role in the pathogenesis of eosinophil infiltration in non-atopic and severe asthmatics. en-copyright= kn-copyright= en-aut-name=TakahashiHisaho en-aut-sei=Takahashi en-aut-mei=Hisaho kn-aut-name=高橋寿保 kn-aut-sei=高橋 kn-aut-mei=寿保 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Bronchial asthma kn-keyword=Bronchial asthma en-keyword=Candida antigen Eosinophil chemotactic factor kn-keyword=Candida antigen Eosinophil chemotactic factor en-keyword=Interleukin-5 kn-keyword=Interleukin-5 END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=5-6 article-no= start-page=599 end-page=611 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The role of eosinophila in the allergic reaction : Morphological changes of reactive eosinophils and immunoglobulin (G&E) receptors kn-title=免疫アレルギー反応における好酸球機能に関する研究―反応好酸球の形態と免疫グロブリンレセプターの検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Eosinophils were isolated from peripheral blood of healthy individuals, asthmatic subjects and patients with PIE syndrome using discontinuous Percoll gradients. Morphological changes of eosinophils stimulated with anti-IgG, anti-IgE and Cal were observed byu the direct count method. Furthermore, the number of immunoglobulin receptors on eosinophils was evaluared by SEM using anti-IgG and anti-IgE linked carboxylate modified latex particles. Eosinophil reactivity after these stimuli was significantly elevated compared to controls. Reactivity was dependent on calcium ions, but FMLP did not affect the reactivity. IgE as well as IgG receptors were significantly expressed on the surface of eosinophils. A positive correlation between the appearance of reactive eosinophils and the expression of these im-munoglobulin receptors was recognized. Correlation in patients with bronchial asthma or PIE syndrome was significantly higher than that in healthy individuals. The appearance of reactive eosinophils after stimulation with anti-IgG was remarkably higher than that after anti-IgE stimulation. The expression of IgG receptore was also higher than that of IgE receptors. These results suggest that obsarving morphological changes in eosinophils may be useful for analyzing the pathogenesis of allergic diseases. en-copyright= kn-copyright= en-aut-name=NkatohHiroshi en-aut-sei=Nkatoh en-aut-mei=Hiroshi kn-aut-name=中東廣志 kn-aut-sei=中東 kn-aut-mei=廣志 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Reactive eosinophil kn-keyword=Reactive eosinophil en-keyword=Discontinuous Percoll gradients kn-keyword=Discontinuous Percoll gradients en-keyword=IgG receptor kn-keyword=IgG receptor en-keyword=IgE receptor kn-keyword=IgE receptor en-keyword=Immune SEM kn-keyword=Immune SEM END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=5-6 article-no= start-page=549 end-page=560 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Clinical studies on plasma cyclic nucleotide levels in acute leukemia kn-title=急性白血病における血漿cyclic nucleotides動態に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Plasma levels of cyclic nucleotides were measured in 71 patients with acute leukemia [50 patients with acute nonlymphocytic leukemia (ANLL), five patients with hypoplastic leuke-mia, and 16 patients with acute lymphocytic leukemia (ALL)], five patients with myelodysplas-tic syndrome (MDS), and 47 healthy volunteers. The cyclic GMP (c-GMP) level, cyclic AMP (c-AMP) level, and c-AMP/c-GMP ratio in healthy volunteers were 15.74±5.10 pmol/ml, 3.20±1.15 pmol/ml and 5.39±2.18, respectively. In the patients with untreated acute leukemia other than hypoplastic leukemia, c-GMP levels were significantly elevated (ANLL : 11.31±13.61 pmol/ml ; ALL : 10.66±7.23 pmol/ml) and c-AMP/c-GMP ratios were significantly reduced (ANLL : 2.01±1.03 ; ALl : 1.66±1.03). These values normalized at remission, than increased or decreased again with recurrence of the disease. Although patients with MDS showed normal c-GMP levels or c-AMP/c-GMP rations, these values were increased or decreased when progression to acute leukemia occurred. There was correlation between c-GMP levels and peripheral leukocyte counts (r=0.429,p<0.05), plasma c-GMP levels and peripheral leukemic cell counts (r=0.412,p<0.05), c-AMP/c-GMP rations and peripheral leukocyte counts (r=-0.577,p<0.05), c-AMP/c-GMP rations and periph-eral leukemic cell counts (r=-0.512,p<0.05), and c-AMP/c-GMP rations and periph-eral leukemic cell counts (r=-0.512,p<0.05), and c-AMP/c-GMP rations and maximum counts of colonies derived from leukemic blast progenitors (r=-0.996,p<0.01). Since plasma levls of cyclic nucleotides reflect the leukemic cell proliferation, measurement of these nucleotides was considered useful for monitoring leukemic cell volume. en-copyright= kn-copyright= en-aut-name=NonakaKenichi en-aut-sei=Nonaka en-aut-mei=Kenichi kn-aut-name=野中研一 kn-aut-sei=野中 kn-aut-mei=研一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=血漿 cyclic nucleotides kn-keyword=血漿 cyclic nucleotides en-keyword=急性白血病 kn-keyword=急性白血病 en-keyword=低形成型白血病 kn-keyword=低形成型白血病 END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=5-6 article-no= start-page=465 end-page=473 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Interleukin-1 production of alveolar macrophages in sarcoidosis kn-title=サルコイドーシスにおける肺胞マクロフｧージの Interleukin-1 産生に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The production of interleukin-1 (IL-1) in alveolar macrophages (AMs) was studied in 25 patients with sarcoidosis to inversigate the role of immune mechanisms in this disease. The radioimmunoassay (RIA) method was compared with the bioassay method for detecting IL-1 in the supernatants of AM cultures. The level of IL-1β measured by RIA was closely corrrelated with the IL-1 activity measured by bioassay (r=0.88, p<0.01). The amount of IL-1β in the supernatant of AM cultures from sarcoidosis patients was compared with those from control subjects. No significant IL-1β activity was detected in the culture supernatants of unstimulated AMs from either group of subjects. However, the production of IL-1β by AMs stimulated with Propionibacterium acnes (P. acnes) was significantly higher in patients with sarcoidosis than in controls (p<0.05). Lipopolysaccharide (LPS) induced increased IL-1β production by AMs from both sarcoidosis patients and controls compared to unstimulated or P. acnes-stimulated AMS, but the mean IL-1β level for sarcoidosis AMs did not differ from that for control AMs. There was no difference in the IL-1β production of peripheral blood monocytes between sarcoidosis patients and controls. These data suggest that AMs are activated in sarcoidosis and may modulate alveolar lymphocyte functions to play a critical role in the pathogenesis of the disease. en-copyright= kn-copyright= en-aut-name=HiokaTohru en-aut-sei=Hioka en-aut-mei=Tohru kn-aut-name=飛岡徹 kn-aut-sei=飛岡 kn-aut-mei=徹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=sarcoidosis kn-keyword=sarcoidosis en-keyword=interleukin-1 kn-keyword=interleukin-1 en-keyword=alveolar macrophage kn-keyword=alveolar macrophage END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=11-12 article-no= start-page=957 end-page=964 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19931231 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on respiratory disorders induced by drug allergy Part 2. Experimental studies of drug allergy in guinea pigs kn-title=薬剤誘起性アレルギー性呼吸器疾患の発症病態に関する研究　第2編　動物モデルによる肺好酸球症の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the pathogenesis of respiratory disease induced by drug allergy, an animal model of eosinophilic lung disease induced by drug was developed. Administration of an aerosol of piperacillin (PIPC) to guinea pigs immunized with emulsion of PIPC and complete Freund's adjuvant (CFA) produced diffuse interstitial lung disease with alveolar wall thickening and alveolitis characterized by marked increase in eosinophils and mononuclear cells. A significant increase of eosinophils in bronchoalveolar lavage fluid was shown in PIPC＋CFA-sensitized animals compared with that in non-sensitized, PIPC-sensitized and CFA-sensitized animals. Using lymphocytes from BAL fluid, drug lymphocyte stimulation test (DLST) revealed a higher stimulation index (S. I.) than that using lymphocytes from peripheral blood tn 5 of seven animals. These findings suggest that eosinophils and lymphocytes (especially lymphocytes sensitized by antigen) play important roles in drug-induced respiratory disease. Furthermore, it is considered that lung lymphocytes were more active than lymphocytes in peripheral blood in the experiment, and local lymphocytes in BAL contributed to the pathogenesis of the respiratory disease induced by drug allergy. en-copyright= kn-copyright= en-aut-name=MifuneTakashi en-aut-sei=Mifune en-aut-mei=Takashi kn-aut-name=御舩尚志 kn-aut-sei=御舩 kn-aut-mei=尚志 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=薬剤性呼吸器疾患 kn-keyword=薬剤性呼吸器疾患 en-keyword=肺好酸球症 kn-keyword=肺好酸球症 en-keyword=動物モデル kn-keyword=動物モデル en-keyword=BAL kn-keyword=BAL en-keyword=DLST kn-keyword=DLST END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=3-4 article-no= start-page=349 end-page=357 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Studies on cytokines produced by alveolar macrophages in the patients with sarcoidosis kn-title=サルコイドーシス患者肺胞マクロファージのサイトカイン産生能に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The role of cytokines produced from alveolar macrophages (AMs) in the compartmentalized T-cell activation in pulmonary sarcoidosis is poorly understood. Herein, we demonstrate the production of Interleukin-6 (IL-6) and tumor necrosis factor (TNF-α) by alveolar macro-phages from 36 patients with sarcoidosis and 26 normal subjects. Non-stimulated AMs from sarcoidosis patients spontaneously produced IL-6, as well as TNF-α. The spontaneous produc-tion of IL-6 was significantly increased in the patients with sarcoidosis than in the normal subjects, but not that of TNF-α. Furthermore, the amount of TNF-α and IL-6 produced from AMs stimulated by Propionibacterium acnes (P. acnes) or lipopolysaccharide was significantly increased in patients with asrcoidosis compared to the normal subjects. TNF-α production from AMs stimulated by P. acnes closely correlated to the IL-6 production from AMs stimulat-ed by P. acnes correlated to thr proportion of lymphocytes in the bronchoalveolar lavage fluid. These findings suggest that AMs are activated in sarcoidosis and that those cells produre IL-6 and TNF-α, which are responsible for T-cell activation. en-copyright= kn-copyright= en-aut-name=ShiomiKatsuhiko en-aut-sei=Shiomi en-aut-mei=Katsuhiko kn-aut-name=塩見勝彦 kn-aut-sei=塩見 kn-aut-mei=勝彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=Interleukin-6 kn-keyword=Interleukin-6 en-keyword=Interleukin-1 kn-keyword=Interleukin-1 en-keyword=tumor necrois factor kn-keyword=tumor necrois factor en-keyword=alveolar macrophage kn-keyword=alveolar macrophage en-keyword=cytokine kn-keyword=cytokine en-keyword=sarocidosis kn-keyword=sarocidosis END start-ver=1.4 cd-journal=joma no-vol=105 cd-vols= no-issue=9-10 article-no= start-page=859 end-page=869 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=199310 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ADF in the lung tissues of HTLV-I associated bronchiolo-alveolar disorder : HABA kn-title=HTLV-I 関連細気管支・肺胞異常症 (HABA) における肺組織中 ADF の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=ATL-derived factor (ADF) is an acid protein, molecular weight about 13KD, which is related to cell classification, activation, and various viral infections. The localization of ADF was studied in the lung tissues of HTLV-I associated bronchiolo-alveolar disorder : HABA, diffuse panbronchiolitis : DPB, and idiopathic interstitial pneumonia : IIP. The localization of ADF in the lung tissues was demonstrated by immunohistochemical staining with anti-ADF antibody as the first antibody, which was made by affinity purification. In all 4 cases of HABA, one of 6 cases of DPB, and two of 5 cases of IIP, staining of the lung tissue was positive. ADF was demonstrated in the bronchial epithelia, alveolar epithelia, vascular endothelia, and desquamative cells in HABA cases as the result of the study. ADF was also restrictively demonstrated in bronchial epithelia in DPB and IIP cases. The case of DPB in which ADF was demonstrated also showed positive HTLV-I related reaction. In conclusion, the localization of ADF in the lung tissues was demonstrated in HABA, DPB and IIP, suggesting immunological abnormalities based upon infections from HTLV-I or a related retrovirus. en-copyright= kn-copyright= en-aut-name=KitamuraTomoki en-aut-sei=Kitamura en-aut-mei=Tomoki kn-aut-name=北村智樹 kn-aut-sei=北村 kn-aut-mei=智樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=HTLV-I Associated Bronchiolo-alveolar Disorder : HABA kn-keyword=HTLV-I Associated Bronchiolo-alveolar Disorder : HABA en-keyword=ATL-derived factor : ADF kn-keyword=ATL-derived factor : ADF en-keyword=Diffuse panbronchiolitis kn-keyword=Diffuse panbronchiolitis en-keyword=Idiopathic interstitial pneumonia kn-keyword=Idiopathic interstitial pneumonia en-keyword=Anti HTLV-I antibody kn-keyword=Anti HTLV-I antibody END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=7-8 article-no= start-page=847 end-page=860 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The mechanism of human basophil activation through the low affinity IgG receptor (Fc γ RU) : Analysis of calcium mobilization using a new flow cytometric method kn-title=低親和性 IgG レセプター（FcγRU）を介するヒト好塩基球の活性化機序に関する研究―フローサイトメーターによるカルシウム動態の解析― en-subtitle= kn-subtitle= en-abstract= kn-abstract=A new method for flow cytometric analysis of calcium mobilization in human peripheral blood basophils without prior purification was developed. The method is based on dual color analysis of centrifugation-enriched mononuclear cell populations using fluo-3 and phycoerythrin (PE)-conjugated CD2, CD14, CD16, CD19 monoclonal antibodies (mAb) to stain contaminated cells. This technique allows the detection of fluo-3 fluorescence as a measure of an increase in the cytoplasmic free calcium concentration ([Ca(2+)]i) while simulataneously discriminating PE-mAb-unlabelled basophils. To clarify whether the human peripheral blood basophil is activated through the low affinity IgG receptor, Fc γ RU, as well as the high affinity IgE receptor, Fc ε　RT, calcium mobilization after Fc γ RU stimulation was analyzed by this method. After cross-linking of Fc γ RU, transient [Ca(2+)]i elevation was observed but there was no apparent difference with interleukin-3(IL-3)-treated cells, and no significant histamine release was observed with or without short pre-incubation of IL-3. These findings suggest that the cross-linking of Fc γ RU, not only Fc ε　RT, can activate human basophils which may result in mediator release other than histamine. en-copyright= kn-copyright= en-aut-name=SuwakiToshimitsu en-aut-sei=Suwaki en-aut-mei=Toshimitsu kn-aut-name=洲脇俊充 kn-aut-sei=洲脇 kn-aut-mei=俊充 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=basophils kn-keyword=basophils en-keyword=FcγRU kn-keyword=FcγRU en-keyword=fluo-3 kn-keyword=fluo-3 en-keyword=Ca(2+) mobilization kn-keyword=Ca(2+) mobilization END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=1-2 article-no= start-page=51 end-page=60 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=The production of platelet activating factor (PAF) from neutrophils in bronchial asthmatics kn-title=気管支喘息における血小板活性化因子の役割に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=It was recently suggested that chemical mediators such as leukotrienes and PAF are more important in the pathogenesis of bronchial asthma than histamine. In this study, to clarify the role of PAF in bronchial asthma, I measured PAF activity derived from human neutrophils by the platelet aggregation tachnique and evaluated this activity in relation to the clinical features of brochial asthma. Neutrophils were separated from heparinized venous blood of 34 bronchial asthmatics and 11 healthy subjects. After suspension at 6×10(6) cells/ml, the cells were treated with 5μg/ml Ca ionophore A23187 for 5 minutes. PAF was extracted from the cell suspension using chloroform, and the activity was measured with an aggregometer using rabbit platelets. PAF activaty was significantly higher (p<0.05) in asthmatics (8.3±6.9 pmol/1×10(6) cells) than in healthy subjects (4.1±3.2 pmol/1×10(6) cells), and tended to increase in steroid-independent asthmatics rather than in steroid-dependent asthma patients. However, there was no difference in PAF production between atopic and non-atopic asthmatics, or between the early and late onset asthmatics. PAF production was increased in patients with severe airway hypersensitivity. These finding suggest that PAF plays an important role in the development and exacerbation of asthma. en-copyright= kn-copyright= en-aut-name=TsujiMitsuaki en-aut-sei=Tsuji en-aut-mei=Mitsuaki kn-aut-name=辻光明 kn-aut-sei=辻 kn-aut-mei=光明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=気管支喘息 kn-keyword=気管支喘息 en-keyword=血小板活性化因子 kn-keyword=血小板活性化因子 en-keyword=好中球 kn-keyword=好中球 END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=7-8 article-no= start-page=837 end-page=846 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Sustaining capacity of hemopoiesis by human stromal cells in bone marrow kn-title=ヒト骨髄間質細胞の造血支持能に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A bone marrow coculturing system developed by M. Y.Gordon was modified for easier evaluation of the function of bone marrow stromal cells. In the method agar was not used, and cells were dried and stained directly on a 35-mm petri dish after culture. The sustaining capacity of hemopoiesis by human stromal cells in bone marrow obtained by this method was compared between eight young and five eldery healthy volunteers. Hemopoietic progenitor cells were obtained from young volunteers and cultures were prepared for seven days. These appeared 86±28 colonies from 10(5) non-adherent bone marrow cells on the sheet of stromal cells from the young volunteers and 89±27 colonies from the eldery. The colonies were classified into two types according to cytochemical appearance through peroxidase staining, but all of them proved to be composed of myeloid cells by enzyme-labeled antibody staining. Colonies were identified easily and accurately by the newly modified coculturing method. No difference in sustaining capacity of hemopoiesis by human stromal cells in bone marrow was found between the young and the eldery using this system. en-copyright= kn-copyright= en-aut-name=UekiKoubun en-aut-sei=Ueki en-aut-mei=Koubun kn-aut-name=植木康文 kn-aut-sei=植木 kn-aut-mei=康文 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部第二内科学教室 en-keyword=human marrow stromal cell kn-keyword=human marrow stromal cell en-keyword=ability to sustain hemopoiesis kn-keyword=ability to sustain hemopoiesis en-keyword=IL-6 production kn-keyword=IL-6 production en-keyword=aging kn-keyword=aging END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=7-8 article-no= start-page=779 end-page=787 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Insulin-like growth factor binding protein-4 (IGFBP-4) and bone growth in childhood kn-title=小児期における Insulin-like growth factor binding protein-4 (IGFBP-4) の骨成長に及ぼす影響 en-subtitle= kn-subtitle= en-abstract= kn-abstract=IGFBP-4 is a 24kD protein, originally isolated from the conditioned medium of human bone cells, and has been implicated as a potent inhibitor of IGF action in vitro. To clarify its biological functions in human bone, I measured the serum concentration of IGFBP-4 from patients with endocrinologic disorders, using Western immunoblot. In normal children, IGFBP-4 increased with age. The serum IGFBP-4 levels of patients with GH deficiency and panhypopituitarism were lower than those of normal children. In addition, the serum levels of IGFBP-4 were also significantly lower in children with Turner's syndrome than in the age-matched normal children who were post pubertal. These findings suggest that GH and the sex steroid increase the production of serum IGFBP-4. Then, I examined the influence of GH on the serum level of of IGFBP-4. In the good responders to GH therapy, IGFBP-4 decreased rapidly during the first month, continued to decrease until the third month, and then increased slowly. On the contrary, in the poor responders to GH therapy, the IGFBP-4 level increased until the third month and then remained at a high level. The increased serum IGFBP-4 level may , at least in part, contribute to the low response to GH therapy in the poor responders. en-copyright= kn-copyright= en-aut-name=YamanakaYoshitaka en-aut-sei=Yamanaka en-aut-mei=Yoshitaka kn-aut-name=山中良孝 kn-aut-sei=山中 kn-aut-mei=良孝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=Insulin-like growth factor binding protein-4 (IGFBP-4) kn-keyword=Insulin-like growth factor binding protein-4 (IGFBP-4) en-keyword=Insulin-like growth factor (IGF-T) kn-keyword=Insulin-like growth factor (IGF-T) en-keyword=骨成長 kn-keyword=骨成長 en-keyword=成長ホルモン kn-keyword=成長ホルモン en-keyword=性ホルモン kn-keyword=性ホルモン END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=1-2 article-no= start-page=1 end-page=10 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=VNTR polymorphism in the 17th and 20th introns of the RB1 gene in Japanese and its application to genetic counseling in hereditary retinoblastoma kn-title=日本人におけるRBI遺伝子第17および20番イントロン内VNTRの多型性の検討および遺伝性網膜芽細胞腫における遺伝相談への応用 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Risk estimation for siblings or offspring is important in genetic counseling of patients with hereditary retinoblastoma. The RB1 gene spans approximately 200 kb in length, containing 27 exons. The use of polymorphic markers within the RB1 gene will eliminate the need of laborious specification of a mutation. The present study determined types and frequencies of VNTR polymorphisms of the 17th and 20th introns of the RB1 gene in 50 unrelated Japanese, using PCR amplification. In the 17th intron VNTR, there were 4 alleles, which ranged from 1400 by to 1550 bp. The most common allele was 1400 bp with a frequency of 73%, and the hetetozygosity rate was 46%. In the 20th intron VNTR, there were at least 9 alleles, wihch ranged from 192 bp to 240 bp. The alleles were more evenly distributed than those of the 17th intron VNTR, and the heterozygosity rate was 64%. These VNTR polymorphisms were successfully applied to the prediction of retinoblastoma and to the determination of parental origin of a chromosome deletion in 3 families with hereditary retinoblastoma. Analysis of VNTR polymorphisms within the RB1 gene proves to be practical and efficient for risk estimation in hereditary retinoblastoma. en-copyright= kn-copyright= en-aut-name=NinomiyaShinsuke en-aut-sei=Ninomiya en-aut-mei=Shinsuke kn-aut-name=二宮伸介 kn-aut-sei=二宮 kn-aut-mei=伸介 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学教室 en-keyword=網膜芽細胞腫 kn-keyword=網膜芽細胞腫 en-keyword=VNTR kn-keyword=VNTR en-keyword=多型 kn-keyword=多型 en-keyword=PCR kn-keyword=PCR en-keyword=遺伝相談 kn-keyword=遺伝相談 END start-ver=1.4 cd-journal=joma no-vol=107 cd-vols= no-issue=9-10 article-no= start-page=205 end-page=217 dt-received= dt-revised= dt-accepted= dt-pub-year=1995 dt-pub=19951031 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Possible protective effects of zine on halothane-induced liver injury in rats kn-title=ラットハロタン肝障害モデルに対する亜鉛の肝障害発生予防作用の可能性 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In a rat model, halothane causes liver injury by interaction with microsomal cytohrome P450 (P450) under a hypoxic condition. Rats that were pretreated with phenobarbital and exposed to halothane under reduced oxygen tension for 2 hours developed hepatic centrilobular necrosis with marked elevation of serum alanine aminotransferase (ALT) 24h after exposure. The elevation of ALT was significantly suppressed in the rats pretreated with a 10mg/kg dose of zinc (Zn) 24h prior to the exposure in comparison with the rats pretreated with saline (control rats). The region of necrosis was also significantly reduced in Zn-pretreated rats. The P450 content of the hepatic microsomes was slightly decreased before the exposure in Zn-pretreated rats in comparison with that in the control rats. It was noteworthy that the aminopyrine demethylation (AD) activity of hepatic microsomal after the exposure in Zn-pretreated rats. These findings indicate that Zn-pratreatment has some protective effect against halothane-induced liver injury and suggest that this protective effect of Zn involcves not only the decrease of P450 before exposure but also the preservation of AD activity during exposure, which result in reduced interaction between P450 and halothane in the microsomes. en-copyright= kn-copyright= en-aut-name=KoyamaYusuke en-aut-sei=Koyama en-aut-mei=Yusuke kn-aut-name=小山祐介 kn-aut-sei=小山 kn-aut-mei=祐介 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部麻酔・蘇生学教室 en-keyword=ハロタン kn-keyword=ハロタン en-keyword=肝障害 kn-keyword=肝障害 en-keyword=亜鉛 kn-keyword=亜鉛 en-keyword=チトクロームP450 kn-keyword=チトクロームP450 END start-ver=1.4 cd-journal=joma no-vol=106 cd-vols= no-issue=7-8 article-no= start-page=717 end-page=730 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=199408 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Cloning and structural analysis of mouse Apex gene encoding the major apurinic/apyrimidinic endonuclease kn-title=マウス APEX ヌクレアーゼ遺伝子のクローニングと構造解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The APEX nuclease is a mammalian multifunctional repair enzyme having 5' apurinic/apyrimidinic (AP) endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3' phosphatase activities. The mouse Apex gene for the enzyme, was isolated from a mouse leukocyte genomic library by plaque hybridization with the mouse Apex cDNA as a probe. The nucleotide sequence of the Apex gene and its 5'-and 3'-flanking regions were determined. With reference to the published Apex cDNA sequence, the mouse Apex gene can be divided into five exons and four introns with a total length of about 2.6 kb. The boundaries between exon and intron follow the GT/AG rule. The translation initiation and termination sites are located in exons U and X, respectively. A part of the 5' flanking region belongs to a CpG island, which extends to intron U. The CpG island is thought to be a putative transcription regulatory region of the Apex gene, a housekeeping gene. en-copyright= kn-copyright= en-aut-name=NagaoKazutaka en-aut-sei=Nagao en-aut-mei=Kazutaka kn-aut-name=長尾一孝 kn-aut-sei=長尾 kn-aut-mei=一孝 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部附属分子細胞医学研究施設病態分子生物学部門 en-keyword=APEX ヌクレアーゼ kn-keyword=APEX ヌクレアーゼ en-keyword=AP エンドヌクレアーゼ kn-keyword=AP エンドヌクレアーゼ en-keyword=DNA 修復酵素 kn-keyword=DNA 修復酵素 en-keyword=Apex 遺伝子（マウス） kn-keyword=Apex 遺伝子（マウス） en-keyword=CpG island kn-keyword=CpG island END start-ver=1.4 cd-journal=joma no-vol=108 cd-vols= no-issue=7-8 article-no= start-page=215 end-page=223 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Serum levels of macrophage colony stimulating factor and other acute phase reactants after open heart surgery kn-title=開心術後における Macrophage Colony-Stimulating Factor と急性相反応物質の血中動態について en-subtitle= kn-subtitle= en-abstract= kn-abstract=To clarify the phathophysiological role of macrophage colony stimulating factor (M-CSF) in the healing processes of inflammation, we studied chronological changes in serum concentrations of M-CSF and other reactants during the acute phase of inflammation in patients who underwent open heart surgery, since these patients showed a precise point of tissue damage. The number of neutrophilic leukocytes increased in the very early postoperative stage, reached a peak 48 h after surgery and declined to the basal level at day 14. The chronological changes in serum C-reactive protein level after surgery were similar to these of neutrophic leukocytes. Serum M-CSF concentrations increased gradually, reached a peak 7 days after surgery and remained at that level until day 14. Based on these phenomena, it is assumed that M-CSF plays a pathophysiological role in the healing processes of an inflammation. Measurement of the serum concentration of M-CSF would be useful for evaluating the process of inflammation. en-copyright= kn-copyright= en-aut-name=ChikakiyoHirokazu en-aut-sei=Chikakiyo en-aut-mei=Hirokazu kn-aut-name=近清裕一 kn-aut-sei=近清 kn-aut-mei=裕一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部公衆衛生学教室 en-keyword=M-CSF kn-keyword=M-CSF en-keyword=開心術 kn-keyword=開心術 en-keyword=白血球数 kn-keyword=白血球数 en-keyword=急性相反応物質 kn-keyword=急性相反応物質 en-keyword=サイトカイン kn-keyword=サイトカイン END start-ver=1.4 cd-journal=joma no-vol=110 cd-vols= no-issue=1-6 article-no= start-page=9 end-page=20 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=19980625 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Localization of Mn- and Cu/Zn-SOD-like immunoreactive cells in the rat brain kn-title=Mn-SOD および Cu/Zn-SOD 様免疫組織化学反応陽性細胞のラット脳内分布 en-subtitle= kn-subtitle= en-abstract= kn-abstract=The localization of manganese (Mn) and copper-zinc (Cu/Zn) superoxide dismutase (SOD)-like immunoreactive (lir) cells in the normal rat brain and the change in numbers of both SOD-lir cortical cells after the injection of ferric chloride into the sensorimotor cortex were investigated immunohistochemically. Large and medium-sized cell bodies, pyramidal to polygonal in shape, were immunostained by anti-Mn-SOD antibody. Most of these were not immunolabeled by anti-GFAP antibody, suggesting that most of the Mn-SOD-lir cells are neurons. These cell bodies were distributed in some selected brain areas with different densities. The densest population of Mn-SOD-lir cells was found in the hypothalamic and limbic structures, and in some brain stem nuclei, such as the thalamic reticular nucleus, the locus ceruleus and the pontine nucleus. Anti-Cu/Zn-SOD antibody immunostained many small round cells throughout both white and gray matter, most of which were also immunolabeled by anti-GFAP antiserum. After intacortical injection of FeCl3, the numbers of Mn- and Cu/Zn-SOD-lir cells per unit area within the adjacent narrow region of the injection site significantly increased 2 and 6 hr after the injection, respectively. en-copyright= kn-copyright= en-aut-name=WangYan en-aut-sei=Wang en-aut-mei=Yan kn-aut-name=王燕 kn-aut-sei=王 kn-aut-mei=燕 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部解剖学第三講座 en-keyword=Mn-SOD kn-keyword=Mn-SOD en-keyword=Cu/Zn-SOD kn-keyword=Cu/Zn-SOD en-keyword=免疫組織化学 kn-keyword=免疫組織化学 en-keyword=ラット脳内分布 kn-keyword=ラット脳内分布 en-keyword=FeCl3　大脳皮質内注入 kn-keyword=FeCl3　大脳皮質内注入 END start-ver=1.4 cd-journal=joma no-vol=111 cd-vols= no-issue=3-8 article-no= start-page=105 end-page=114 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990831 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Expression of DNA topoisomerase T and Uβ in the developing cerebellar plate in rat embryos kn-title=胎児期における小脳皮質の発生とDNAトポイソメラーゼTおよびUβの発現 en-subtitle= kn-subtitle= en-abstract= kn-abstract=DNA topoisomerases are enzymes that catalyze topological changes of DNA by transiently cleaving one (topo T) and a pair of complementary DNA strands (topo Uβ), participating in cellular transactions of DNA. Expression of both enzymes in the developing cerebellar plate in rat embryos was studied immunohistochemically. Neuroepitheliad cells in the ventricular layer expressed topo T and topo Uβ on embryonic day 15 (E16) and E16, respectively. On E 16-22, cells in the intermediate layer (IML) are known to undergo extensivc differentiation to become Purkinje cells and deep cerebellar neurons. Many IML cells, immunostained with not only anti-topo T and-Uβ but also-calbindin antibodies, were differentiating and migrationg to form the Purkinje cell layer. Cells in the external germinal layer (EGL) on E17, originating from the germinal trigone and spreading over the surface of cerebellar plate, expressed topo Ton E17. Topo Uβ immunoreactive cells, however, were not readily recognized in EGL untill E22. The topo Uβ immunopositive cells resided in the ventral half of the layer, corresponding to the premigratory zone of postnatal EGL. These date suggest the involvement of topo Tand Uβ in DNA metabolism associated with cerebellar development in embryos, especially that of topo Uβ in the processes of neuronal differentiation. en-copyright= kn-copyright= en-aut-name=OgawaMakoto en-aut-sei=Ogawa en-aut-mei=Makoto kn-aut-name=小川誠 kn-aut-sei=小川 kn-aut-mei=誠 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部解剖学第三講座 en-keyword=ラット小脳発生 kn-keyword=ラット小脳発生 en-keyword=小脳板 kn-keyword=小脳板 en-keyword=DNA トポイソメラーゼT kn-keyword=DNA トポイソメラーゼT en-keyword=DNA トポイソメラーゼUβ kn-keyword=DNA トポイソメラーゼUβ en-keyword=免疫組織化学 kn-keyword=免疫組織化学 END start-ver=1.4 cd-journal=joma no-vol=109 cd-vols= no-issue=7-12 article-no= start-page=133 end-page=140 dt-received= dt-revised= dt-accepted= dt-pub-year=1997 dt-pub=19971225 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Expression of parathyroid-related peptide mRNA in the kidney kn-title=腎臓における副甲状腺ホルモン関連ペプチドの局在の検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=PTHrP mRNA was quantified by RT-PCR and the localization of PTHrP mRNA was determined by in situ hybridization in kidney. During development, the expression of PTHrP mRNA is much higher before 2 weeks of age while kidney is developing. In E16 fetal mice, PTHrP mRNA was strongly expressed in the collecting ducts, urothelium of the pelvis, tubles, the glomeruli and immature elements in the cortex of the developing kidney (nephrogenic zone). within 0-day-old-mice, it was dominantly expressed in collecting ducts, the urothelium of the pelvis and nephrogenic zone. These findings suggest that PTHrP plays a role in kidney development as an autocrine/ paracrine factor. en-copyright= kn-copyright= en-aut-name=AyaKunihiko en-aut-sei=Aya en-aut-mei=Kunihiko kn-aut-name=綾邦彦 kn-aut-sei=綾 kn-aut-mei=邦彦 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部小児科学講座 en-keyword=副甲状腺ホルモン関連ペプチド（PTHrP) kn-keyword=副甲状腺ホルモン関連ペプチド（PTHrP) en-keyword=腎臓 kn-keyword=腎臓 en-keyword=発達 kn-keyword=発達 en-keyword=in situ hybridization kn-keyword=in situ hybridization END start-ver=1.4 cd-journal=joma no-vol=114 cd-vols= no-issue=2 article-no= start-page=167 end-page=171 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20020930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Helicobacter pylori decreases mucin synthesis in human gastric mucosa via inhibution of UDP-galactosyltansferase kn-title=Helicobacter Pylori 感染胃粘膜における胃ムチン生合成の変化 ―UDP-ガラクトース転移酵素活性測定による検討― en-subtitle= kn-subtitle= en-abstract= kn-abstract=Background/aims : Alterations of gastric mucin have been postulated as important pathogenic properties of Helicobacter pylori. In this study, we investigated gastric mucin synthesis in H. pylori-infected gastric mucosa by measuring UDP-galactosyltransferase (UDP-Gal-T) activity, a key enzyme for the synthesis of mucin, and the amount of intracellular mucin in the gastric mucosa. Methods : Gastric biopsy specimens were obtained from thirty-seven patients(20 H. pylori-positive and 17 H. pylori-negative). UDP-Gal-T activity of the biopsy specimens was measured by an assay aystem we had developed, using a peanut agglutinin lectin. The amount of intracellular mucin in the gastric epithrlial cells was analyzed by measuring the cells' periodic acid-Schiff-alcian blue staining-positive subtances. Results : UDP-Gal-T activites in the antral mucosa of H. Pylori-positive patients were significantly lower than that of H. pylori-negative patients (p<0.05). The amount of intracelluar mucin in the antral epithelial cells of H. pylori-positive patients was significantly lower than that of H. pyolori-negative patients (p<0.01). Conclusions : H. pylori infection decreases gastric mucin synthesis by the inhibition of UDP-Gal-T activity. This effect may impair the gastric mucosal barrier and contribute to the mucosal injury induced by H. pylori infection. en-copyright= kn-copyright= en-aut-name=TanakaShoichi en-aut-sei=Tanaka en-aut-mei=Shoichi kn-aut-name=田中彰一 kn-aut-sei=田中 kn-aut-mei=彰一 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=Department of Medicine and Medical Scinece,Okayama University Graduate School of Medicine and Dentistry en-keyword=Helicobacter pylori kn-keyword=Helicobacter pylori en-keyword=胃粘膜 kn-keyword=胃粘膜 en-keyword=ムチン生合成 kn-keyword=ムチン生合成 en-keyword=ガラクトース転移酵素 kn-keyword=ガラクトース転移酵素 END start-ver=1.4 cd-journal=joma no-vol=113 cd-vols= no-issue=1 article-no= start-page=1 end-page=16 dt-received= dt-revised= dt-accepted= dt-pub-year=2001 dt-pub=20010428 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Establishment of an adriamycin-resistant human bladder cancer cell line (T-24/ADM) and analysis of the mechanism of resistance kn-title=Adriamycin 耐性ヒト膀胱癌培養細胞株の樹立と耐性機序に関する研究 en-subtitle= kn-subtitle= en-abstract= kn-abstract=A human bladder cencer cell line resistant to adriamycin (ADM), T-24/ADM was establishied in vitro by exposing T-24 parent cells to a progressively higher concentration of the drug over an 18 month period. The T-24/ADM was 34.9 times more resistant to ADM than the T-24 parent. The T-24/ADM exhibited cross resistance to ADM derivatives, vinca alkaloid (vindesine, vincristine), etoposide and SN-38, but collateral sensitivity to methotrexate. The biological and biochemical characteristics of T-24/ADM were examined in terms of ADM-resistance. Although a flow cytometric analysis showed that Pglycoprotein is not expressed on the T-24/ADM cells, lower accumalation of the drug caused by decreased uptake and increaced active efflux were observed. The cellular level of glutathione-S-transferase π was 1.8-fold higher than the parent cells and the activity of nuclear extracts of DNA topoisomerase U for T-24/ADM assayed by decatenation of kinetoplast DNA was lower, about one-half that of the T-24 parent. Confocal laser microscopy revealed the difference in intracellular distribution of ADM in T-24/ADM; in particular, the accumlation of the drug in the nucleus decreased. Additionally western blot analysis showed an enhanced expression of multidrug resistance-associated protein (MRP) in the T-24/ADM cells. This resistant cell may be used as an experimantal system to elucidate the mechanisum of ADM resistance and also as a model for developing new chemotherapeutic strategies against multi-drug resistant bladder cancer. en-copyright= kn-copyright= en-aut-name=AkebiNaoki en-aut-sei=Akebi en-aut-mei=Naoki kn-aut-name=野比直樹 kn-aut-sei=野比 kn-aut-mei=直樹 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学医学部泌尿器科学教室 en-keyword=adriamycin resistance kn-keyword=adriamycin resistance en-keyword=human bladder cancer cell line kn-keyword=human bladder cancer cell line en-keyword=multidrug resistance kn-keyword=multidrug resistance END start-ver=1.4 cd-journal=joma no-vol=114 cd-vols= no-issue=1 article-no= start-page=39 end-page=51 dt-received= dt-revised= dt-accepted= dt-pub-year=2002 dt-pub=20020530 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=A mechanism of development of arterial thrombosis : β2-glycoprotein I-specific ligand and autoantibody kn-title=抗リン脂質抗体症候群における動脈血栓の発症機序：β2-グリコプロテインIに特異的なリガンドと自己抗体の関与 en-subtitle= kn-subtitle= en-abstract= kn-abstract=β2-Glycoprotein I (β2-GPI) is a major antigen for anticardiolipin antibodies (aCL) appeared in patients with antiphospholipid syndrome (APS). We recently reported that β2-GPI specifically binds to oxidized low-density lipoprotein (oxLDL) and that on of the β2-GPI's major ligands derived from oxLDL, oxLig-1, is 9-(7-ketocholest-5-en-3β-yloxy)-9-oxononanoic acid (J. Lipid Res. 42, 697, 2001). In the present study, it was demonstrated that carboxylated variants of cholesteryl linoleate have a critical role for β2-GPI binding.In vitro experiments indicate that oxLDL was uptaken by macrophages via an interaction among the ligand such as oxLig-1, β2-GPI, and anti-β2-GPI autoantibodies. The uptake was not occurred by cholesterol or its ester without a free carboxyl residue, i.e., cholesteryl lenoleate, by cholesterol, or by 7-ketocholesterol alone, even in the presence of β2-GPI and anti-β2-GPI antibodies. Thus, carboxyl variants of cholesteryl ester specific for β2-GPI may mediate anti-β2-GPI Ab-dependent uptake of oxLDL by macrophages and autoimmune atherogenesis developed in APS. en-copyright= kn-copyright= en-aut-name=LiuQingping en-aut-sei=Liu en-aut-mei=Qingping kn-aut-name=劉慶平 kn-aut-sei=劉 kn-aut-mei=慶平 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学院医歯学総合研究科細胞化学分野 en-keyword=antiphospholipid syndrome (APS) kn-keyword=antiphospholipid syndrome (APS) en-keyword=atherosclerosis kn-keyword=atherosclerosis en-keyword=autoantibody kn-keyword=autoantibody en-keyword=β2-glycoprotein I (β2-GPI) kn-keyword=β2-glycoprotein I (β2-GPI) en-keyword=oxidized LDL (oxLDL) kn-keyword=oxidized LDL (oxLDL) END start-ver=1.4 cd-journal=joma no-vol=120 cd-vols= no-issue=2 article-no= start-page=159 end-page=168 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080801 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Examination of dialysis patients by a systematic review focused on serum phosphorus value kn-title=透析患者における至適血清リン値に関する文献による検討 en-subtitle= kn-subtitle= en-abstract= kn-abstract=In November 2006, The Japanese Society for Dialysis Therapy (JSDT) announced its first guideline, "The guideline for the management of secondary hyperparathyroidism in chronic dialysis patients," which gives the recommended range of management target values, especially for serum phosphorus, corrected serum calcium and serum intact parathyroid hormone concentrations. Recent studies have suggested that these factors are independently associated with mortality, especially increased cardiovascular mortality. In this research we focused on the serum phosphorus concentration because it is the highest clinical factor among these three. We systematically reviewed almost all documents that discussed the relation between serum phosphorus concentration and mortality as well as the "range of the management target value" specified by foreign guidelines from the US, UK, Canada, Australia and other countries. We summarized the finding concerning the serum phosphorus value of dialysis patients (especially the upper bound value). As a result, it was found that the "range of the management target value" varied among these guidelines. The reasons for this variation likely included differences in the measurement day, in the categories of serum phosphorus concentration, and in the exposure conditions among the studies to which the guidelines referred. Moreover, it was concluded that the Japanese guideline announced by the JSDT should be updated based on the results of future studies. en-copyright= kn-copyright= en-aut-name=TsukijiMakoto en-aut-sei=Tsukiji en-aut-mei=Makoto kn-aut-name=築地淳 kn-aut-sei=築地 kn-aut-mei=淳 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院環境学研究科　生命環境学専攻　国際保健学分野 en-keyword=透析患者 (dialysis patients) kn-keyword=透析患者 (dialysis patients) en-keyword=血清リン値 (phosphatemia) kn-keyword=血清リン値 (phosphatemia) en-keyword=CKD-MBD kn-keyword=CKD-MBD en-keyword=ガイドライン (guideline) kn-keyword=ガイドライン (guideline) en-keyword=生命予後 (prognosis) kn-keyword=生命予後 (prognosis) en-keyword=JSDT kn-keyword=JSDT END start-ver=1.4 cd-journal=joma no-vol=119 cd-vols= no-issue=3 article-no= start-page=285 end-page=292 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=20080104 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=II Standard treatment for advanced lung cancer kn-title=II 肺癌の内科的治療 en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=KiuraKatsuyuki en-aut-sei=Kiura en-aut-mei=Katsuyuki kn-aut-name=木浦勝行 kn-aut-sei=木浦 kn-aut-mei=勝行 aut-affil-num=1 ORCID= en-aut-name=TakigawaNagio en-aut-sei=Takigawa en-aut-mei=Nagio kn-aut-name=瀧川奈義夫 kn-aut-sei=瀧川 kn-aut-mei=奈義夫 aut-affil-num=2 ORCID= en-aut-name=OzeIsao en-aut-sei=Oze en-aut-mei=Isao kn-aut-name=尾瀬功 kn-aut-sei=尾瀬 kn-aut-mei=功 aut-affil-num=3 ORCID= en-aut-name=YasugiMasayuki en-aut-sei=Yasugi en-aut-mei=Masayuki kn-aut-name=八杉昌幸 kn-aut-sei=八杉 kn-aut-mei=昌幸 aut-affil-num=4 ORCID= en-aut-name=OchiNobuaki en-aut-sei=Ochi en-aut-mei=Nobuaki kn-aut-name=越智宣昭 kn-aut-sei=越智 kn-aut-mei=宣昭 aut-affil-num=5 ORCID= en-aut-name=HaradaDaijiro en-aut-sei=Harada en-aut-mei=Daijiro kn-aut-name=原田大二郎 kn-aut-sei=原田 kn-aut-mei=大二郎 aut-affil-num=6 ORCID= en-aut-name=TanimotoMitsune en-aut-sei=Tanimoto en-aut-mei=Mitsune kn-aut-name=谷本光音 kn-aut-sei=谷本 kn-aut-mei=光音 aut-affil-num=7 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=2 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=3 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=4 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=5 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=6 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 affil-num=7 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 血液・腫瘍・呼吸器内科学 en-keyword=放射線化学療法 kn-keyword=放射線化学療法 en-keyword=分子標的治療 kn-keyword=分子標的治療 en-keyword=血管新生阻害薬 kn-keyword=血管新生阻害薬 en-keyword=受容体チロシンキナーゼ阻害薬 kn-keyword=受容体チロシンキナーゼ阻害薬 END start-ver=1.4 cd-journal=joma no-vol=119 cd-vols= no-issue=2 article-no= start-page=147 end-page=151 dt-received= dt-revised= dt-accepted= dt-pub-year=2007 dt-pub=20070903 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Characterization of the binding of botulinum type B 16S toxin to human intestinal epithelial cells kn-title=ボツリヌスB型16S毒素のヒト腸管上皮細胞への結合活性の解析 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Botulinum neurotoxin produced by Clostridium botulinum type B is a complex of 12S and 16S toxins. 12S toxin consists of a neurotoxin and a nontoxic non-HA (NTNH). The 16S toxin consists of a neurotoxin, an NTNH, and a hemagglutinin (HA). Food-borne botulism is caused by these complex toxins, which are ingested orally and absorbed from the digestive tract across the epithelial barrier lining the gut. Here we show that the type B 16S toxin, but not the 12S toxin or the neurotoxin, binds to the T84 human intestinal epithelial cell line. We also demonstrate that the HA moiety in the 16S toxin mediates the toxin binding to the cells. The carbohydrates containing a galactose moiety inhibited the binding of the 16S toxin to the T84 cells, and neuraminidase treatment of the cells increased the 16S toxin binding. The binding of the 16S toxin to the neuraminidase-treated cells was also inhibited by carbohydrates containing a galactose moiety. These results suggest that the type B 16S toxin binds to human intestinal epithelial cells via the galactose moiety in the carbohydrate chain on the cell surface. en-copyright= kn-copyright= en-aut-name=JinYingji en-aut-sei=Jin en-aut-mei=Yingji kn-aut-name=金英姫 kn-aut-sei=金 kn-aut-mei=英姫 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学大学院医歯薬学総合研究科 病原細菌学 en-keyword=ボツリヌス毒素　(botulinus toxin) kn-keyword=ボツリヌス毒素　(botulinus toxin) en-keyword=Clostridium botulinum kn-keyword=Clostridium botulinum en-keyword=ボツリヌス中毒 (botulism) kn-keyword=ボツリヌス中毒 (botulism) en-keyword=T84細胞 (T84 cell) kn-keyword=T84細胞 (T84 cell) en-keyword=ガラクトース (galactose) kn-keyword=ガラクトース (galactose) END start-ver=1.4 cd-journal=joma no-vol=97 cd-vols= no-issue=1 article-no= start-page=1 end-page=7 dt-received= dt-revised= dt-accepted= dt-pub-year=2008 dt-pub=200802 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification, Characterization and Crystal Structure of Isoamylase from Thermophilic Bacteria Rhodothermus marinus kn-title=海産性好熱性細菌 Rhodothermus marinus 由来イソアミラーゼ の精製，性質検討及びＸ線結晶構造解析 en-subtitle= kn-subtitle= en-abstract=Rhodothermus marinus 由来イソアミラーゼ遺伝子を組み込んだプラスミド pBX２を使用し，大腸菌 Top10株を形質転換し，16時間の前培養，24時間の本培養後，菌体破砕し，得られた無細胞抽出液を熱処理（80℃，10 min），50ｵ硫安分画，陰イオン交換カラムクロマトグラフィー（DEAEﾝトヨパール），ハイドロキシアパタイトカラムクロマトグラフィーに供して本酵素の精製を行った．本精製酵素の性質検討を行った結果，本酵素の最適反応温度は70℃，pH４であり，また本酵素は60℃で１時間処理しても活性が低下することが無く，Pseudomonas amyloderamosa 由来イソアミラーゼよりも高い耐熱性を有することが判明した．本酵素の結晶化・X線結晶構造解析を行った結果，本酵素は P. amyloderamosa 由来イソアミラーゼと同様Ｎドメイン・ＡドメインＣドメインの３つのドメインから構成されており，活性残基（Ｄ359，Ｅ395，Ｄ467）など活性中心付近のアミノ酸残基も P. amyloderamosa 由来イソアミラーゼと同様，高度に保存されていた．本酵素の熱安定性が P. amyloderamosa 由来イソアミラーゼよりも高 い要因として，P. amyloderamosa 由来イソアミラーゼよりもループの長さが全体的に短いことと，カルシウムイオン結合サイトの欠如が挙げられた．今後さらに構造解析を進めることにより，本酵素の熱安定性機構，反応 機構など更なる知見が得られることが期待される． kn-abstract=The isoamylase gene from Rhodothermus marinus was cloned into and expressed in Escherichia coli Top 10. As a result of characterization of purified R. marinus isoamylase. the enzyme had an optimum pH of 4.0 and optimum temperature of 70℃. Thermal inactivation studies of the purified R. marinus isoamylase revealed the enzymatic activity to be uninfluenced after one hour incubation at 60℃. These results suggest that R. marinus isoamylase has high thermostability. The crystallization and crystal structure analysis of R. marinus isoamylase was performed. The three-dimensional structure at 1.9? resolution was determined in complex with the panose. R. marinus isoamylase is composed of three domains N, A and C, and, has a (β/α)8-barrel in domain A. The secondary structural alignments of the R. marinus isoamylase and P. amyloderamosa isoamylase was carried out. They have the four active-site consensus regions characteristic of the α-amylase family. And the essential residue of the α-amylase family (D359, E395, and D467) was conserved in these enzymes. R. marinus isoamylase has shorter loops than P. amyloderamosa isoamylase. And R. marinus isoamylase had no Ca2+ binding site. These results are thought to be factors of thermostability of R. marinus isoamylase. en-copyright= kn-copyright= en-aut-name=TachibanaAkiko en-aut-sei=Tachibana en-aut-mei=Akiko kn-aut-name=立花亜紀子 kn-aut-sei=立花 kn-aut-mei=亜紀子 aut-affil-num=1 ORCID= en-aut-name=TamuraTakashi en-aut-sei=Tamura en-aut-mei=Takashi kn-aut-name=田村隆 kn-aut-sei=田村 kn-aut-mei=隆 aut-affil-num=2 ORCID= en-aut-name=ImadaKatsumi en-aut-sei=Imada en-aut-mei=Katsumi kn-aut-name=今田勝巳 kn-aut-sei=今田 kn-aut-mei=勝巳 aut-affil-num=3 ORCID= en-aut-name=KinoshitaMiki en-aut-sei=Kinoshita en-aut-mei=Miki kn-aut-name=木下実紀 kn-aut-sei=木下 kn-aut-mei=実紀 aut-affil-num=4 ORCID= en-aut-name=NambaKeiichi en-aut-sei=Namba en-aut-mei=Keiichi kn-aut-name=難波啓一 kn-aut-sei=難波 kn-aut-mei=啓一 aut-affil-num=5 ORCID= en-aut-name=TsutsumiNoriko en-aut-sei=Tsutsumi en-aut-mei=Noriko kn-aut-name=堤紀子 kn-aut-sei=堤 kn-aut-mei=紀子 aut-affil-num=6 ORCID= en-aut-name=HashidaMiyoko en-aut-sei=Hashida en-aut-mei=Miyoko kn-aut-name=橋田みよ子 kn-aut-sei=橋田 kn-aut-mei=みよ子 aut-affil-num=7 ORCID= en-aut-name=SakaguchiHiromichi en-aut-sei=Sakaguchi en-aut-mei=Hiromichi kn-aut-name=坂口博脩 kn-aut-sei=坂口 kn-aut-mei=博脩 aut-affil-num=8 ORCID= en-aut-name=InagakiKenji en-aut-sei=Inagaki en-aut-mei=Kenji kn-aut-name=稲垣賢二 kn-aut-sei=稲垣 kn-aut-mei=賢二 aut-affil-num=9 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=大阪大学 affil-num=4 en-affil= kn-affil=大阪大学 affil-num=5 en-affil= kn-affil=大阪大学 affil-num=6 en-affil= kn-affil=ノボザイムズ ジャパン affil-num=7 en-affil= kn-affil=ノボザイムズ ジャパン affil-num=8 en-affil= kn-affil=ノボザイムズ ジャパン affil-num=9 en-affil= kn-affil=岡山大学 en-keyword=isoamylase kn-keyword=isoamylase en-keyword=Rhodothermus marinus kn-keyword=Rhodothermus marinus en-keyword=crystal structure kn-keyword=crystal structure en-keyword=thermostability kn-keyword=thermostability END start-ver=1.4 cd-journal=joma no-vol=9 cd-vols= no-issue=1 article-no= start-page=65 end-page=68 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=20040227 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Numerical Simulation of a Flow Induced by a Pumping-up Facilities in a Lake kn-title=湧昇循環装置によって誘起される湖沼内流れの数値シミュレーション en-subtitle= kn-subtitle= en-abstract= kn-abstract=The purpose of this research is solving the flow in a lake which caused by a fan who rotates at the low speed set on the water surface for the purpose of water quality purification of a lake. Although it is shown clearly by various experiments that the water quality in a lake improved by operation of this kind of equipment, the mechanism of the flow was not fully understood. In this research simulation of the flow which was caused by this equipment was carried out numerically, and the aspect of the flow corresponding to various conditions or the form of a lake was investigated. en-copyright= kn-copyright= en-aut-name=SuitoHiroshi en-aut-sei=Suito en-aut-mei=Hiroshi kn-aut-name=水藤寛 kn-aut-sei=水藤 kn-aut-mei=寛 aut-affil-num=1 ORCID= en-aut-name=NoukaMasakatsu en-aut-sei=Nouka en-aut-mei=Masakatsu kn-aut-name=苗加昌克 kn-aut-sei=苗加 kn-aut-mei=昌克 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Fluid dynamics kn-keyword=Fluid dynamics en-keyword=Numerical simulation kn-keyword=Numerical simulation END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=129 end-page=134 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Properties of α-Glucodidase from Taro Tuber kn-title=サトイモのα-グルコシダーゼの精製と性質 en-subtitle= kn-subtitle= en-abstract=里芋塊茎を破砕し、その抽出液からα−グルコシダーゼを硫安分画、エチルアルコール分画、CM−セルロファインカラムトグラフィー、調製用ディスク電気泳動により約2,500倍に精製した。本酵素はニゲロースに対してはマルトースと同程度に作用したが、イソマルトースに対する作用は非常に弱く、トレハロースにはまったく作用しなかった。本酵素は少糖類や多糖類にもよく作用し、マルトヘキサオースや可溶性澱粉に対してはマルトースと同程度の相対速度比と、むしろ低めのKm値を示した。このことから本酵素が生体内で澱粉分解に密接に関わっていることが推測された。 kn-abstract=α-Gulcosidase (EC 3.2.1.20) has been purified 2,500-fold taro (Colocasia esculanta Shott) tuber by a procedure incluting fractionation with ammonium sulfate and ethyl alcohl, CM-cellulofine column chromatography, and preparative disc gel electrophoresis. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch. However, the enzyme hydrolyzed isomaltose only very weakly. The Km values of the enzyme for maltohexaose and soluble starch were lower than that for maltose. en-copyright= kn-copyright= en-aut-name=MashimaHideyuki en-aut-sei=Mashima en-aut-mei=Hideyuki kn-aut-name=間島英之 kn-aut-sei=間島 kn-aut-mei=英之 aut-affil-num=1 ORCID= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=2 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=α-glucosidase kn-keyword=α-glucosidase en-keyword=taro tuber kn-keyword=taro tuber en-keyword=Colocasia esculanta Shott kn-keyword=Colocasia esculanta Shott END start-ver=1.4 cd-journal=joma no-vol=5 cd-vols= no-issue=2 article-no= start-page=121 end-page=127 dt-received= dt-revised= dt-accepted= dt-pub-year=1998 dt-pub=1998 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ナメクジのα-グルコシダーゼの精製と性質 kn-title=Purification and Properties of α-Glucosidase from Slugs en-subtitle= kn-subtitle= en-abstract=ナメクジには多くのグリコシダーゼが存在し、摂取する植物多糖の代謝に関与している。その中で著量に存在し、澱粉代謝に関わっているα−グルコシダーゼについて研究を行った。ナメクジを破砕し、得られたα−グルコシダーゼを硫安分画、セファクリルS−200HRカラムクロマトグラフィー、CM−セルロースカラムトグラフィー、調製用ディスク電気泳動により精製した。本酵素はセファクリルS-200HRカラムトグラフィーで2種（T、U）に分画された。T画分はCM−セルロースクロマトグラフィーでさらに2種（T−1、T−2）に分画された。得られた3種のα−グルコシダーゼはマルトースやマルトオリゴ糖によく作用したが、イソマルトースに対する作用は弱かった。3種の中でも最も多量に存在するα−グルコシダーゼUは他の酵素（T−1、T−2）よりも可溶性澱粉に対する作用が顕著で、マルトースよりも強く作用した。 kn-abstract=Three forms of α-glucosidase(EC3.2.1.20), designated as T, U,V,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase V　hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase T hyrolyzed soluble starch more slowly. en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=1 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Slug kn-keyword=Slug en-keyword=Incilaria bilineata kn-keyword=Incilaria bilineata en-keyword=α-glucosidase kn-keyword=α-glucosidase END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=239 end-page=252 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Purification and Characterization of α-Glucosidases from Spinach Seeds kn-title=ホウレンソウ（Spinacia oleracea L.）種子のαグルコシダーゼの精製とその性質 en-subtitle= kn-subtitle= en-abstract=α−グルコシダーゼの分子多型が各種クロマトグラフィーによりホウレンソウ種子から単離された。α−グルコシダーゼT、U、V、Wの分子量は、それぞれSDS−PAGEにより78、78、82、82kDA、ゲル濾過のより62,62,190、70kDであった。α-グルコシダーゼTとUは、可溶性デンプンを基質とした際のKm値がマルトースよりも約10倍低く、さらに、α−グルカンだけでなくマルトオリゴ糖に対しても高い活性を示すことから、同じような酵素的特性を持つことが示された。最適pHは4.5ｶﾗ5.5を示し、温度安定性は70℃、20分処理で約50％の残存活性を示した。一方、α−グルコシダーゼVとWは、マルトースを基質とした際のKm値が可溶性デンプンよりも3−4倍低い値を示し、マルトオリゴ糖に対しては高い活性を示すが、α−グルカンに対してはほとんど活性を示さないことから、同じような酵素的特性を有することが示された。最適pHは4.5−5.5、そして70℃、20分処理では活性が認められなかった。しかしながら、抗α−グルコシダーゼV血清は、α−グルコシダーゼVに対してのみ特異的に沈降線を形成した。 kn-abstract=Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases T,U,V,and W were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Tand U showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases V and W showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase V serum precipitated specifically with α-glucosidase V. en-copyright= kn-copyright= en-aut-name=SugimotoManabu en-aut-sei=Sugimoto en-aut-mei=Manabu kn-aut-name=杉本学 kn-aut-sei=杉本 kn-aut-mei=学 aut-affil-num=1 ORCID= en-aut-name=FuruiSatoshi en-aut-sei=Furui en-aut-mei=Satoshi kn-aut-name=古井聡 kn-aut-sei=古井 kn-aut-mei=聡 aut-affil-num=2 ORCID= en-aut-name=SuzukiYukio en-aut-sei=Suzuki en-aut-mei=Yukio kn-aut-name=鈴木幸雄 kn-aut-sei=鈴木 kn-aut-mei=幸雄 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 en-keyword=α-Glucosidase kn-keyword=α-Glucosidase en-keyword=Spinach kn-keyword=Spinach en-keyword=Seed kn-keyword=Seed en-keyword=Spinacia oleracea L. kn-keyword=Spinacia oleracea L. en-keyword=Molecular form kn-keyword=Molecular form END start-ver=1.4 cd-journal=joma no-vol=4 cd-vols= no-issue=2 article-no= start-page=119 end-page=135 dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=1996 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Orchid Fleck Virus, the Causal Agent of a Yellowish Fleck Mosaic Disease of Calanthe kn-title=エビネ類に発生する黄色斑紋モザイク病の病原，Orchid Fleck Virusについて en-subtitle= kn-subtitle= en-abstract=1988年以来、山口・宮崎・鹿児島県で発生が認められたエビネ類（エビネ、タカネ、ヒゼン、サツマ、ツルラン、オナガエビネ）の葉に淡緑色〜黄色斑紋のモザイク病の病原ウイルスを調べたところ、orchid fleck virusと同定された。本ウイルスは11科46種（54品種）の植物に汁液接種を行ったところ、エビネに感染して原株と同様の病班を生じたほか、C.quinoa,フダンソウに全身感染し、C.murale,ホウレンソウ、ツルナ、ササゲ、タバコ、N.clevelandii,N.glutinosa,N.rusticaの5科12種の植物に局部感染が認められた。本ウイルスの粒子はDN法試料で被膜のない弾丸型であり、またときに短桿菌型も存在したが、その大きさは長さが約105〜125nm、幅約45〜50nmであった。病葉の粗汁液を用いた希釈限度は10-3〜10-4、不活性化温度は45〜50℃、保存限度は1〜2時間であった。ウイルスの汁液接種には、接種源植物としてC.quinoa またはツルナがよく、病葉磨砕後夏期では7分以内、冬期では15分以内の病汁液を供試すると接種検定によい結果が得られることが認められた。純化ウイルスを家兎に注射して、微滴法で力値512倍の抗血清が得られた。本抗血清はCymbidium から分離されたOFV・Cy-50とよく反応し、寒天ゲル内二重拡散法では Cal.94-16およびOFV・Cy-50の沈降帯が完全に融合した。ウイルスの構造蛋白質の分子量は約55Kであった。病細胞の超薄切片の電顕観察像には、閣内にviroplasmが認められ、その内部や周辺に層状に集塊あるいは散在した粒子が認められた。さらに核内や細胞質内に膜に包まれた車輪状の粒子集塊も見られた。OFVによる本病をエビネ類黄色斑紋モザイク病とした。 kn-abstract=Orchid fleck virus(OFV) was isolated from Calanthe spp.(Cal. discolor,Cal. Bicolor,Cal. Hizen,Cal. triplicata,Cal longicalcarata,Cal Satusma) showing light-green and/or yellowish fleck mosaic on the leaves, which different from previously known viruses of Calanthe. OFV caused systemic infection in Calanthe, Chenopodium quinoa and Beta vulgasis var. cicla, and local infection in C.amaranticolor, C. murale, Spinacia oleracea, Tetragonia expansa, Nicotiana tabacum, N. clevelandii, N. glutinasa, N. rustica, Vigna unguiculata. C quinoa and T expansa are useful as indecator hosts and as a source of virus for inoculation, diagnosis and purification. Sap from C. quinoa was infective after dilution to 10-3 but not 10-4, after 10 min at 45 but not 50℃, and after 1 hr at 20℃ but not 2 hrs. For sap inoculation, it is best to use the homogenate of OFV-onfected leaves within about 7-8 min after homogenization in summer and within about 15 min in winter. The virus particles were bullet-shape or bacilliform, approximately 45-50×105-125 nm in a negatively stained praparations. In ultrathin sections, the viroplasms were observed in the nuclei, and the virus particles and the chracteristic spokewheel structures were found both in the nuclei and the cytoplasm. Antiserum (precipitin tiner:1/512) against the present virus reacted strongly with the isolates of OFV-Cy-50, similar to that of homologous virus. In agar gel diffusion tests, no spur formation occurred among Cal. 94-16 and OFV-Cy-50. In SDS-polyacrylamide gel electrophoresis, one major band of Mr 55,000, probably viral nucleocapsid-protein, and three minor proteins were detected, similar to those of OFV･So from Cymbidium. en-copyright= kn-copyright= en-aut-name=InouyeNarinobu en-aut-sei=Inouye en-aut-mei=Narinobu kn-aut-name=井上成信 kn-aut-sei=井上 kn-aut-mei=成信 aut-affil-num=1 ORCID= en-aut-name=MatsumotoJun-ichi en-aut-sei=Matsumoto en-aut-mei=Jun-ichi kn-aut-name=松本純一 kn-aut-sei=松本 kn-aut-mei=純一 aut-affil-num=2 ORCID= en-aut-name=MaedaTakanori en-aut-sei=Maeda en-aut-mei=Takanori kn-aut-name=前田孚憲 kn-aut-sei=前田 kn-aut-mei=孚憲 aut-affil-num=3 ORCID= en-aut-name=MitsuhataKoji en-aut-sei=Mitsuhata en-aut-mei=Koji kn-aut-name=光畑興二 kn-aut-sei=光畑 kn-aut-mei=興二 aut-affil-num=4 ORCID= en-aut-name=KondoHideki en-aut-sei=Kondo en-aut-mei=Hideki kn-aut-name=近藤秀樹 kn-aut-sei=近藤 kn-aut-mei=秀樹 aut-affil-num=5 ORCID= en-aut-name=TaharaMochimu en-aut-sei=Tahara en-aut-mei=Mochimu kn-aut-name=田原望武 kn-aut-sei=田原 kn-aut-mei=望武 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Calanthe kn-keyword=Calanthe en-keyword=Orchid fleck virus kn-keyword=Orchid fleck virus en-keyword=Calanthe yellowish fleck disease kn-keyword=Calanthe yellowish fleck disease END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=167 end-page=180 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ゼニゴケ葉緑体遺伝子と相同性を持つラン藻　Synechocystis PCC6803株のORF326、frxC およびORF469を標的にした変異の導入 kn-title=Targeted Mutagenesis of ORF326, frxC and ORF469 of a Cyanobacterium, Synechocysitis PCC6803, Homologous to Liverwort Chloroplast Genes en-subtitle= kn-subtitle= en-abstract=ゼニゴケ葉緑体ORF316、frxCおよびORFと相同性を持つ、形質転換型ラン藻Synechocystis PCC6803株のORF326、frxCおよびORF469の機能についての情報を得るため、これらの欠損株の作製を行った。コード領域にカナマイシン耐性遺伝子カセットを挿入することにより不活性化された変異型のORF（オーブンリーディングフレーム）を持つプラスミドを用いてSynechocystis PCC6803株の形質転換を行い、カナマイシンを含む培地で選抜した。Synechocystis PCC6803株は約10コピーのクロモソームを持つが、サザンブロット解析の結果、ORF326については、変異型と野生型のORF326の双方を持つ株しか得られず、増殖に必要と推測された。一方frxCおよびORF469については、ともに全て変異型に置き換わった株が得られ、増殖には必要ないことが示された。さらにORF469欠損株を光照射下で培養し、増殖速度とクロロフィルa濃度を測定したが、いずれも野生株とほぼ同じであり、ORF469は、この条件下では、増殖やクロロフィル生合成に必要ないと推察された。 kn-abstract=ORF326, frxC and ORF469 of a transfomable cynobacterium, Synechcystis PCC6803, have sequence similarity with ORF465 on the choroplast genome of a livewort, Marchantia polymorpha, respectively. To elucidate their functions,targeted mutagenesis was performed by transformation with clened DNA in which the ORF was disrupted by insertion of a kanamycin resistancen gene cassette.Streak-purifications of a single colony of each transformant were repeatde to segregate homozygous mutants for disrupted copies, because Synechocystis PCC6803 was reported to have approximately 10 chromosomal DNA copies. Southern blot analysis revealed that mutants for ORF326 had not only disrupted ORF326 copies but also wild type ORF326 copies. This suggests that ORF326 is indispensable for growth under the mixotrophic growth condition used. However, mutants for frxC and mutants for ORF469 had only mutated copies, indicating that they dispensable for growth. Growth and chlorophyll a content of an ORF469-disrupted mutant were compared and chlorophyll a content of an ORF469-disrupted mutant were compared to those of wild type under mixotrophic growth condition, but no significant difference was detected. This indicates that ORF469 is required for neither normal growth nor chlorophyll biosynthesis under thie condition. en-copyright= kn-copyright= en-aut-name=OguraYutaka en-aut-sei=Ogura en-aut-mei=Yutaka kn-aut-name=小倉豊 kn-aut-sei=小倉 kn-aut-mei=豊 aut-affil-num=1 ORCID= en-aut-name=TakemuraMiho en-aut-sei=Takemura en-aut-mei=Miho kn-aut-name=竹村美保 kn-aut-sei=竹村 kn-aut-mei=美保 aut-affil-num=2 ORCID= en-aut-name=OdaKenji en-aut-sei=Oda en-aut-mei=Kenji kn-aut-name=小田賢司 kn-aut-sei=小田 kn-aut-mei=賢司 aut-affil-num=3 ORCID= en-aut-name=OhyamaKanji en-aut-sei=Ohyama en-aut-mei=Kanji kn-aut-name=大山莞爾 kn-aut-sei=大山 kn-aut-mei=莞爾 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Cyanobacteria kn-keyword=Cyanobacteria en-keyword=Gene disruption kn-keyword=Gene disruption en-keyword=Synechocystis PCC6803 kn-keyword=Synechocystis PCC6803 END start-ver=1.4 cd-journal=joma no-vol=2 cd-vols= no-issue=2 article-no= start-page=149 end-page=157 dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=1994 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Increase in Cytochorome c and a 11.9 kDa protein in Submerged Rice Seedlings after Exposure to Air kn-title=イネ水中芽生えの酸素適応過程におけるチトクロムcと１１．９kDaタンパク質の増加 en-subtitle= kn-subtitle= en-abstract=イネ水中芽生えを空気に触れさせた後のチトクロムc含量の変動を調べるために、米ぬかから精製したチトクロムcを用いて抗チトクロムc血清を作成した。ウェスタンプロット分析によると、水中芽生えにはチトクロムcは検出されなかったが、空気に触れてから6時間以降に検出された。酸素適応24時間の芽生えには生重量あたりで好気対照と同レベルのチトクロムcが存在した。抗血清作成の抗原として用いたチトクロムc標品は、A408/A280比の値（4.66）が高いことから、よく精製されていると考えられるが、抗血清はいくつかの他のポリペプチドと反応した。そのうちの一つはチトクロムcよりも抗血清に強く反応し、その分子量は11.9kDaであった。このポリペプチドは酸素適応過程で増加し、水中芽生えや好気対照では24時間酸素適応芽生えよりも少なかった。 kn-abstract=To examine the changes in cytocrome c content in submerged rice seedlings after exposure to air, antiserum was prepared against purified cytocrome c from rice bran. Western blottong analysis revealed that cytochrome c was detected 6 h after exposure to air, but not detected in submerged rice seedling. On a fresh weight basis, the same level of cytochrome c as that of the aerobic control was found in the 24-h-air adapted seedlings. judging from the high A408/A280 ratio (4.66),the cytochrome c preparation used as antigen was considered to be well purified. However, the antiserum reacted other several polypeptides. One of them reacted more strongly against the antisermu than cytochrome c and its molecular weight was estimated as 11.9 kDa. The polypeptide increased during air adaptation and the levels found in both submerged seedlings and aerobic control were lower than that in 24-h-air-adapted seedlings. en-copyright= kn-copyright= en-aut-name=ShibasakaMineo en-aut-sei=Shibasaka en-aut-mei=Mineo kn-aut-name=柴坂三根夫 kn-aut-sei=柴坂 kn-aut-mei=三根夫 aut-affil-num=1 ORCID= en-aut-name=UshimaruTakashi en-aut-sei=Ushimaru en-aut-mei=Takashi kn-aut-name=丑丸敬史 kn-aut-sei=丑丸 kn-aut-mei=敬史 aut-affil-num=2 ORCID= en-aut-name=OokuboKatsuyuki en-aut-sei=Ookubo en-aut-mei=Katsuyuki kn-aut-name=大久保勝之 kn-aut-sei=大久保 kn-aut-mei=勝之 aut-affil-num=3 ORCID= en-aut-name=TsuchidaShin-ichi en-aut-sei=Tsuchida en-aut-mei=Shin-ichi kn-aut-name=土田進一 kn-aut-sei=土田 kn-aut-mei=進一 aut-affil-num=4 ORCID= en-aut-name=TsujiHideo en-aut-sei=Tsuji en-aut-mei=Hideo kn-aut-name=辻英夫 kn-aut-sei=辻 kn-aut-mei=英夫 aut-affil-num=5 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 en-keyword=Air-adaptation kn-keyword=Air-adaptation en-keyword=Anti-cytochrome c serum kn-keyword=Anti-cytochrome c serum en-keyword=Cytochrome c kn-keyword=Cytochrome c en-keyword=Cytocrome c purification kn-keyword=Cytocrome c purification en-keyword=Oryza sativa kn-keyword=Oryza sativa END start-ver=1.4 cd-journal=joma no-vol=1 cd-vols= no-issue=2 article-no= start-page=159 end-page=166 dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=1993 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 kn-title=Purification and Properties of Wall-bound α-Glucosidase from Suspension-cultured Sugar-beet Cells en-subtitle= kn-subtitle= en-abstract=テンサイ培養細胞には数種のα‐グルコシダーゼが存在している。相当量のα‐グルコシダーゼ活性は細胞破壊後、緩衝液と食塩で抽出されるが、まだかなりのα‐グルコシダーゼ活性が細胞残渣に含まれたままである。その酵素は、界面活性剤やS-S結合切断試薬では遊離されなかったが、細胞壁分解酵素により可溶化された。テンサイ培養細胞にスミチームCとペクトリアーゼY-23を作用させると、細胞はプロトプラストになり、細胞壁由来の成分のみを分離できた。可溶化された部分からα‐グルコシダーゼを硫安分画、セファクリルS-200HRカラムクロマトグラフィー、CM-セルロースカラムクロマトグラフィーにより精製した。本酵素は、マルトース、ニゲロース、マルトオリゴ糖、可溶性澱粉に良く作用したが、それらに比べ、イソマルトースに対する作用は弱かった。本酵素は、マルトオリゴ糖と可溶性澱粉にマルトースよりも強く作用した。本酵素以外にテンサイ培養細胞とテンサイ種子から数種類のα‐グルコシダーゼが単離されているが、本酵素の基質的特異性はそれらのものと異なっていた。 kn-abstract=Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. en-copyright= kn-copyright= en-aut-name=YamasakiYoshiki en-aut-sei=Yamasaki en-aut-mei=Yoshiki kn-aut-name=山崎良樹 kn-aut-sei=山崎 kn-aut-mei=良樹 aut-affil-num=1 ORCID= en-aut-name=KonnoHaruyoshi en-aut-sei=Konno en-aut-mei=Haruyoshi kn-aut-name=今野晴義 kn-aut-sei=今野 kn-aut-mei=晴義 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=Beta vulgaris L. cv. kn-keyword=Beta vulgaris L. cv. en-keyword=Tsukisappu kn-keyword=Tsukisappu en-keyword=Sugar-beet kn-keyword=Sugar-beet en-keyword=Wall-bound enzyme kn-keyword=Wall-bound enzyme en-keyword=α-Glucosidase kn-keyword=α-Glucosidase en-keyword=Protoplast kn-keyword=Protoplast END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=31 end-page=38 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=エンドウ原形質膜におけるATPアーゼとホスファチジルイノシトールリン資質リン酸化酵素の共精製 kn-title=Co-purification of Plasma Membrane ATPase and Phosphatidylinositol Kinase from Pea Plasma Membranes en-subtitle= kn-subtitle= en-abstract=エンドウの上胚軸組織から分離した原形質膜画分におけるATPアーゼとホスファチジルイノシトールリン脂質リン酸化酵素との相互作用を解析する目的で、双方の原形質膜画分からの可溶化とそれらの部分精製を試みた。原形質膜のTritonX−100可溶化画分をグリセロール連続密度勾配遠心分画に供し、得られた活性画分をさらに分子ふるいカラムクロマトグラフィーによって分離した。この結果、ＡＴＰアーゼとホスファチジルイイノシトールリン脂質リン酸化酵素は共精製され、非変性条件下では双方の活性を分けることができなかった。 kn-abstract=The plasma membrance ATPase was partially purified by a linear glycerol density gradient centrifugation of the detergent-solubilized plasma membrance poteins and subsequent separation by a size-exclusion column chromatogrphy. A purified ATPase preparation is shown to contain a 97.6kDa protein that was cross-reacted with an antibody raised against mung bean H+-ATPase. The preparation also exhibited the phosphorylation of exogenous phosphatidylionsitol(PI) when supplized with [γ-32P]ATP. These results indicate that one form plasma membrance ATPase is co-purified with PI kinase. en-copyright= kn-copyright= en-aut-name=ToyodaKazuhiro en-aut-sei=Toyoda en-aut-mei=Kazuhiro kn-aut-name=豊田和弘 kn-aut-sei=豊田 kn-aut-mei=和弘 aut-affil-num=1 ORCID= en-aut-name=IchinoseYuki en-aut-sei=Ichinose en-aut-mei=Yuki kn-aut-name=一瀬勇規 kn-aut-sei=一瀬 kn-aut-mei=勇規 aut-affil-num=2 ORCID= en-aut-name=YamadaTetsuji en-aut-sei=Yamada en-aut-mei=Tetsuji kn-aut-name=山田哲治 kn-aut-sei=山田 kn-aut-mei=哲治 aut-affil-num=3 ORCID= en-aut-name=ShiraishiTomonori en-aut-sei=Shiraishi en-aut-mei=Tomonori kn-aut-name=白石友紀 kn-aut-sei=白石 kn-aut-mei=友紀 aut-affil-num=4 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 en-keyword=Lipid kinase kn-keyword=Lipid kinase en-keyword=Mycosphaerella kn-keyword=Mycosphaerella en-keyword=plasma membrance ATPase kn-keyword=plasma membrance ATPase en-keyword=pea(Pisum sativum L.) kn-keyword=pea(Pisum sativum L.) en-keyword=suppressor kn-keyword=suppressor END start-ver=1.4 cd-journal=joma no-vol=88 cd-vols= no-issue=1 article-no= start-page=13 end-page=17 dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=199902 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation and Purification of Nocardamine from the Validamycin Producer kn-title=バリダマイシン生成菌培養液から抗細菌性抗生物質ノカルダミンの分離・精製 en-subtitle= kn-subtitle= en-abstract=Streptomyces hygroscopicus subsp. limoneus はバリダマイシン生成菌として知られているが、その抗カビ物質以外に抗細菌性抗生物質を生成することは知られていない。われわれはその微生物がバリダマイシン非生成条件において生成する抗細菌性抗生物質を見出そうと試みた。その結果、グラム陰性細菌に活性を示す抗生物質EO-3を得たのでこれを分離・精製してノカルダミンと同定した。本報告では本抗生物質の培養条件、抗菌スペクトル、精製および同定方法について述べる。尚、この研究は平成6年度から8年度までの3年間に亙る岡山大学学内特定研究『特殊環境生物の機能開発と物質生産への応用』を分担して行ったものである。 kn-abstract=An antibacterial active against P. mirabilis was isolated from the culture of Streptomyces hygroscopicus subsp. limoneus, validamycin producer. The antibiotic was found to be produced with a non-validamycin producing condition. The antibiotic was identified as nocardamine with the analytical data, IR, 1H-NMR and 13C-NMR spectra. en-copyright= kn-copyright= en-aut-name=HigashideEiji en-aut-sei=Higashide en-aut-mei=Eiji kn-aut-name=東出栄治 kn-aut-sei=東出 kn-aut-mei=栄治 aut-affil-num=1 ORCID= en-aut-name=OmatsuYoshiharu en-aut-sei=Omatsu en-aut-mei=Yoshiharu kn-aut-name=大松義治 kn-aut-sei=大松 kn-aut-mei=義治 aut-affil-num=2 ORCID= en-aut-name=InoueSuemi en-aut-sei=Inoue en-aut-mei=Suemi kn-aut-name=井上末瑞 kn-aut-sei=井上 kn-aut-mei=末瑞 aut-affil-num=3 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉伸 kn-aut-sei=木村 kn-aut-mei=吉伸 aut-affil-num=4 ORCID= en-aut-name=KanzakiHiroshi en-aut-sei=Kanzaki en-aut-mei=Hiroshi kn-aut-name=神崎浩 kn-aut-sei=神崎 kn-aut-mei=浩 aut-affil-num=5 ORCID= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Streptomyces kn-keyword=Streptomyces en-keyword=antibacterial compoud kn-keyword=antibacterial compoud en-keyword=nocardamine kn-keyword=nocardamine en-keyword=non-validamycin producing condition kn-keyword=non-validamycin producing condition END start-ver=1.4 cd-journal=joma no-vol=89 cd-vols= no-issue=1 article-no= start-page=9 end-page=14 dt-received= dt-revised= dt-accepted= dt-pub-year=2000 dt-pub=200002 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=登熟期ヒマ種子ミクロゾーム画分からのアリル-α-マンノシダーゼの精製と酵素学的諸性質 kn-title=Purification and Properties of Aryl-α-mannosidase from Microsomal Fraction of Developing Ricinus communis Endosperms en-subtitle= kn-subtitle= en-abstract=登熟期ヒマ種子のミクロゾーム画分からp-nitrophenyl-α-D-mannopyranoside(PNP-α-Man)及び蛍光標識N-グリカンに対して活性を示すα-マンノシダーゼを精製後、酵素学的諸性質を解析した。精製酵素は還元条件下のSDS-PAGEでは43kDaの相対分子量を示し、PNP-α-Manを基質とした場合の至適反応条件は50-60℃、pH4.5-5.0であった。本酵素はEDTAにより阻害を受けたが、Zn2+及びCa2+添加により活性が回復することから金属イオン要求性の酵素であると思われる。また、PNP-α-Manに対するKm値は1.3mM、Man5GLcNAc2-PAに対するKm値は0.4mMであった。本酵素はMan5GlcNAc2-PAに作用しMan4GlcNAc2-PAを誘導するものの、Man6GlcNAc2-PA対してむしろ強い活性を示し、Man5GlcNAc2-PA(88%)及びMan4GlcNAc2-PA(12%)を誘導した。しかしながら、Man4GlcNAc2-PA,GlcNAc1Man5GlcNAc2-PA、Man4Xy11GlcNAc2-PAは本酵素の有効な基質とはなり得なかった。これらの結果から、本酵素はα-1,2-マンノース残基を優先的に加水分解し、その後、β-結合マンノースに結合するα-1,3マンノース残基を遊離することが示唆された。 kn-abstract=An α-mannosidase, which would be involved in N-linked glycoprotein metabolism, was purified and characterized from microsomal fraction of developing Ricinus communis endosperms. The purified enzyme with 43 kDa on SDS-PAGE showed maximal activity at pH5.0 and 50℃, when p-nitrophenyl-α-mannopyranoside was used as a substrate. α-Mannosidase activity was inhibited by EDTA and the reduced activity was rescued by addition of Zn2+ or Ca2+, suggesting this α-mannosidase should be a metal enzyme. Ricinus aryl-α-mannosidase was able to convert the Man6GlcNAc2-PA and Man5GlcNAc2-PA to Man4GlcNAc2-PA but was completely inactive toward Man4GlcNAc2-PA, Man4Xy11GlcNAc2-PA and GlcNAc1Man5GlcNAc2-PA. en-copyright= kn-copyright= en-aut-name=YamaiMasafumi en-aut-sei=Yamai en-aut-mei=Masafumi kn-aut-name=山井雅文 kn-aut-sei=山井 kn-aut-mei=雅文 aut-affil-num=1 ORCID= en-aut-name=KimuraYoshinobu en-aut-sei=Kimura en-aut-mei=Yoshinobu kn-aut-name=木村吉信 kn-aut-sei=木村 kn-aut-mei=吉信 aut-affil-num=2 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 en-keyword=plant α-mannosidase kn-keyword=plant α-mannosidase en-keyword=plant glycoprotein kn-keyword=plant glycoprotein en-keyword=N-glycan metabolism kn-keyword=N-glycan metabolism END start-ver=1.4 cd-journal=joma no-vol=93 cd-vols= no-issue=1 article-no= start-page=1 end-page=4 dt-received= dt-revised= dt-accepted= dt-pub-year=2004 dt-pub=200402 dt-online= en-article= kn-article= en-subject= kn-subject= en-title= kn-title=Ageratum conyzoidesに含まれる殺線虫活性物質 en-subtitle= kn-subtitle= en-abstract= kn-abstract=Activity-guided chromatographic purification of the methanol extract of Ageratum conyzoides, using pine wood nematodes Bursaphelenchus xylophilus successfully led to the isolation and characterization of the nematicidal compound with minimum effective dose (MED) of 75 μg/ cotton ball (μg/bl.). Based on the chemical and spectral properties, the compound was determined to be coumarin (1). The activity of coumarin derivatives (2-5) was also investigated. en-copyright= kn-copyright= en-aut-name=NakajimaShuhei en-aut-sei=Nakajima en-aut-mei=Shuhei kn-aut-name=中島修平 kn-aut-sei=中島 kn-aut-mei=修平 aut-affil-num=1 ORCID= en-aut-name=TakaishiKazuto en-aut-sei=Takaishi en-aut-mei=Kazuto kn-aut-name=高石和人 kn-aut-sei=高石 kn-aut-mei=和人 aut-affil-num=2 ORCID= en-aut-name=NaganoYouichi en-aut-sei=Nagano en-aut-mei=Youichi kn-aut-name=長野庸一 kn-aut-sei=長野 kn-aut-mei=庸一 aut-affil-num=3 ORCID= en-aut-name=AlenYohannes en-aut-sei=Alen en-aut-mei=Yohannes kn-aut-name=アレンヨハネス kn-aut-sei=アレン kn-aut-mei=ヨハネス aut-affil-num=4 ORCID= en-aut-name=KawazuKazuyoshi en-aut-sei=Kawazu en-aut-mei=Kazuyoshi kn-aut-name=河津一儀 kn-aut-sei=河津 kn-aut-mei=一儀 aut-affil-num=5 ORCID= en-aut-name=BabaNaomichi en-aut-sei=Baba en-aut-mei=Naomichi kn-aut-name=馬場直道 kn-aut-sei=馬場 kn-aut-mei=直道 aut-affil-num=6 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=岡山大学 affil-num=3 en-affil= kn-affil=岡山大学 affil-num=4 en-affil= kn-affil=岡山大学 affil-num=5 en-affil= kn-affil=岡山大学 affil-num=6 en-affil= kn-affil=岡山大学 en-keyword=Nematicidal compound kn-keyword=Nematicidal compound en-keyword=Ageratum conyzoides kn-keyword=Ageratum conyzoides en-keyword=Coumarin kn-keyword=Coumarin en-keyword=Cotton kn-keyword=Cotton END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=妊娠認識物質ウシインターフェロン(bIFN)τの大量生産･精製:胚発育促進への適用 kn-title=LARGE-SCALE PRODUCTION AND PURIFICATION OF BIOLOGICAL ACTIVE BOVINE INTERFERON τ: POSSIBLE APPLICATION TO INCREASE EMBRYONIC DEVELOPMENT en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=TakahashiHitomi en-aut-sei=Takahashi en-aut-mei=Hitomi kn-aut-name=高橋ひとみ kn-aut-sei=高橋 kn-aut-mei=ひとみ aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2005 dt-pub=20050325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=銀杏種子由来のα-マンノシダーゼとβ-キシロシダーゼの精製と酵素学的諸性質に関する研究:糖鎖生物学への応用利用 kn-title=Studies on the purification and characterization of α-mannosidase and β-xylosidase from Ginkgo biloba seeds: Application to Glycobiology en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=WooKwan Kit en-aut-sei=Woo en-aut-mei=Kwan Kit kn-aut-name=ウークワン キット kn-aut-sei=ウー kn-aut-mei=クワン キット aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1999 dt-pub=19990325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=乳酸菌によって生産される新規抗菌性物質の精製とその特性 kn-title=Purification and characteristics of novel antibacterial substance from lactic acid bacteria en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=睦宗洙 kn-aut-sei=睦 kn-aut-mei=宗洙 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19930328 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=CM-グリシニン消化性ダイズ種子プロテアーゼの精製と酵素的性質 kn-title=Purification and Characterization of CM-glycinin Digesting Protease from Soybean Seeds en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=モハマドアクタルジャマン kn-aut-sei=モハマド kn-aut-mei=アクタルジャマン aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=2003 dt-pub=20030331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ジストニア患者治療用の完全に活性化されたボツリヌスB型毒素の精製法 kn-title=Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name=ArimitsuHideyuki en-aut-sei=Arimitsu en-aut-mei=Hideyuki kn-aut-name=有満秀幸 kn-aut-sei=有満 kn-aut-mei=秀幸 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1993 dt-pub=19930328 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=気管支喘息における好塩基球の動態に関する研究 第1編フローサイトメトリーを用いたヒト末梢血好塩基球の高純度分離法について) 第2編各種サイトカインによるヒト好塩基球の遊走活性に関する検討) kn-title=1. Purification of human blood basophils using negative selection by flow cytometry ; 2. Effects of cytokines on human basophil chemotaxis en-subtitle= kn-subtitle= en-abstract= kn-abstract=1.Basophils were purified from peripheral blood of normal donors using Percoll discontinuous gradients and negative selection by flow cytometry. The mean purity of basophils obtained was 84.7 +/- 4.1 (s.d.)% (range 77.3-90.0%, n = 13). The overall yield of these procedures was 16.0 +/- 2.6% (range 11.0-19.9%, n = 13), and cell viability of purified basophils exceeded 90%. Properties of highly purified basophils obtained by flow cytometry did not differ from those of partially enriched basophil preparations from Percoll discontinuous gradients in respect of: (i) intracellular histamine content; (ii) percentage of spontaneous histamine release in buffer; and (iii) percentage of histamine release triggered by ionophore A 23187 or anti-IgE. Moreover, purified basophils responded chemotactically to complement C5a in a dose-dependent manner. These findings suggest that our procedure for purification of human basophils does not affect the functions of basophils and may be useful for in vitro studies on the role of basophils in hypersensitivity reactions such as bronchial asthma. ; 2.Basophil chemotactic activity (BCA) of eight recombinant human (rh) cytokines was examined. Highly purified basophils were obtained by Percoll discontinuous gradients, followed by negative selection using flow cytometry. Then BCA was measured by means of modified Boyden chamber method. Both interleukin (IL)-3 and granulocyte-macrophage colony-stimulating factor (GM-CSF) had much more potent BCA than complement C5a, leukotriene B4 and platelet activating factor, well known as granulocyte chemotactic factors. Chemotaxis rather than chemokinesis was shown in chequerboard analysis of basophil migration induced by IL-3 and GM-CSF. Relatively high concentrations of IL-5 also induced basophil migration, although predominantly chemokinetic. IL-8 had apparent BCA, which was not so high as that of C5a. In contrast, IL-2, IL-4, interferon(IFN)-gamma and granulocyte colony-stimulating factor (G-CSF) had no significant BCA. These findings suggest that IL-3, IL-5, GM-CSF and, perhaps, IL-8 have an effect on basophil migration as well as modulation of basophil mediator release and may provide some insight into the basophil accumulation observed in late-phase allergic responses. en-copyright= kn-copyright= en-aut-name=TanimotoYasushi en-aut-sei=Tanimoto en-aut-mei=Yasushi kn-aut-name=谷本安 kn-aut-sei=谷本 kn-aut-mei=安 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1982 dt-pub=19820331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ラット肝上清分画のcysteine aminotransferaseの精製と性質 kn-title=Purification and characterization of cysteine aminotransferase from rat liver cytosol en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=赤木玲子 kn-aut-sei=赤木 kn-aut-mei=玲子 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1986 dt-pub=19860930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=肝細胞膜特異抗原(LCM) 精製および性状について kn-title=A LIVER CELL MEMBRANE SPECIFIC ANTIGEN (LCM) ITS PURIFICATION AND CHARACTERIZATION en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=森近茂 kn-aut-sei=森近 kn-aut-mei=茂 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=マウス腹水肉腫細胞の39KDa APエンドヌクレアーゼの精製と性状解析 kn-title=Purification and Characterization of a 39 KDa AP endonuclease from mouse ascites sarcoma cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=若林肇 kn-aut-sei=若林 kn-aut-mei=肇 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1987 dt-pub=19870930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=遠心流出法によるイヌ肝類洞壁細胞の分離とそれら細胞の同種移植肝拒絶反応における免疫学的役割 kn-title=Purification of Canine Sinusoidal Lining Cells by Centrifugal Elutriation and Their Possible Role in Liver Allograft Rejection en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=戸田耕太郎 kn-aut-sei=戸田 kn-aut-mei=耕太郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1994 dt-pub=19940331 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=樹状細胞分化因子の部分精製,及びその性質についての検討 kn-title=Partial Purification and Characterization of dendritic Cell Differentiation Factor en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=宮谷克也 kn-aut-sei=宮谷 kn-aut-mei=克也 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1988 dt-pub=19880930 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=ヒトのインスリン抗体の生物学的意義に関する研究 第1編:モノコンポーネントインスリンをリガンドとしたアフｨニテｨークロマトグラフｨーによるインスリン結合抗体の精製) 第2編: 純化されたヒトのインスリン抗体がラット遊離脂肪細胞におけるグルコース取り込みに及ぼす影響 kn-title=Purification of insulin-binding antibody by affinity chromatography using monocomponent insulin as ligand. en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=宮井陽一郎 kn-aut-sei=宮井 kn-aut-mei=陽一郎 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol= cd-vols= no-issue= article-no= start-page= end-page= dt-received= dt-revised= dt-accepted= dt-pub-year=1996 dt-pub=19960325 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=一本鎖切断と塩基欠落部位の修復に関与する哺乳類DNA 修復酵素) 第1編(酸素ラジカルによDNA 一本鎖切断とその哺乳類細胞の修復酵素)第2編(マウス腹水肉腫細胞のAPエンドヌクレアーゼ/修復ジエステラーゼの精製と性状解析 kn-title=Mammalian　DNA　repair　enzymes　involved　repair　of　single-strand　breaks　and　abasic　sites　　第1編　Oxygen　radical-induced　single-strand　DNA　breaks　and　repair　of　the　damage　by　a　cell-　free　system　第2編　Purification　and　characterization　of　an　AP　endonuclease/DNA3'repair　diesterase　from　mouse　ascites　sarcoma　cells en-subtitle= kn-subtitle= en-abstract= kn-abstract= en-copyright= kn-copyright= en-aut-name= en-aut-sei= en-aut-mei= kn-aut-name=MohamedAltaf kn-aut-sei=Mohamed kn-aut-mei=Altaf aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=49 cd-vols= no-issue=1 article-no= start-page=35 end-page=44 dt-received= dt-revised= dt-accepted= dt-pub-year=1977 dt-pub=1977 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Effect of Carotenoid on Leaf Lipoxygenase Activity I. : Purification of Spinach Chloroplast Lipoxygenase and the Inhibition by Lutein to Its Activity kn-title=緑葉リポキシゲナーゼ活性に及ぼすカロチノイドの影響I. ホウレンソウ葉リポキシゲナーゼの精製とルテインによる活性阻害 en-subtitle= kn-subtitle= en-abstract= kn-abstract=ホウレン草クロロプラストからリポキシゲナーゼを抽出し,CM celluloseカラムクロマトグラフィー及びSephadex G-200によるゲルクロマトグラフィーによって精製を行った. その結果比活性は25倍に増大した. 粗酵素及びこの精製標品を用いてこのリポキシゲナーゼの諸性質を調べた. 作用最適pHは7.0であり,アルカリ側では活性低下が大きい. 尿素は活性にほとんど影響を及ぼさない. 抗酸化剤としてのNDGAは完全に失活させ,チオ尿素は16.6%活性を低下させる. Cu2+Pb2+はいずれも活性を阻害するが,Cu2+の場合に濃度が2×10-4Mを越えると逆に酸素吸収活性を増大させる. これは自動酸化によるものであろう. ルテインはリポキシゲナーゼに働らいて活性を低下させる. WchとDchの活性に対するルテインの影響はDchの場合に大きいので,リポキシゲナーゼはクロロプラストの表面ではなく内部に存在すると考えられる. Triton×-100で可溶化した酵素標品もルテインによって活性低下が認められ,その際ルテインは褪色する. この褪色はきわめて速かであり,数分でplateau状態になるが,酸素吸収活性は5分を過ぎてから,ルテイン区と対照 区との間に差が認められ始めるので,ルテインによる酸索吸収活性阻害はルテインよりもむしろ褪色生成物によるものと考えられる。 en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= en-aut-name=MatsugamiMichio en-aut-sei=Matsugami en-aut-mei=Michio kn-aut-name=松上道雄 kn-aut-sei=松上 kn-aut-mei=道雄 aut-affil-num=2 ORCID= en-aut-name=MoritokiTsutomu en-aut-sei=Moritoki en-aut-mei=Tsutomu kn-aut-name=守時勤 kn-aut-sei=守時 kn-aut-mei=勤 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=ポーラ化成(株) affil-num=3 en-affil= kn-affil=ブンセン(株) END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=44 end-page=46 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=1974 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Isolation of Crystalline Lutein from Spinach Leaves kn-title=ホウレンソウ葉からLuteinの結晶単離について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Up to date, any precise reports on crystalline lutein, which was comprised at about 20% in carotenoids of green leaves and at about 40% in these xanthophylls, have not been found. In previous report, the authors showed that the glycocarotenoids containing galactose were existent in spinach leaves, and in the processes of these carotenoids purification lutein was found to be crystallized easily from cold acetone in considerably good yields. This crystalline lutein had mp. 174-176℃, and E1cm 1% of 2344 at 446 nm. Furthermore, visible absorption spectrum, IR-spectrum, NMR-spectrum and Mass spectrum were also estimated for the identification of it. en-copyright= kn-copyright= en-aut-name=TakagiShigeaki en-aut-sei=Takagi en-aut-mei=Shigeaki kn-aut-name=高木茂明 kn-aut-sei=高木 kn-aut-mei=茂明 aut-affil-num=1 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 END start-ver=1.4 cd-journal=joma no-vol=44 cd-vols= no-issue=1 article-no= start-page=28 end-page=36 dt-received= dt-revised= dt-accepted= dt-pub-year=1974 dt-pub=1974 dt-online= en-article= kn-article= en-subject= kn-subject= en-title=Influence of Environmental Temperature on Growth of Japanese Quails kn-title=日本ウズラの成長に及ぼす温度環境の影響について en-subtitle= kn-subtitle= en-abstract= kn-abstract=Up to date, any precise reports on crystalline lutein, which was comprised at about 20% in carotenoids of green leaves and at about 40% in these xanthophylls, have not been found. In previous report, the authors showed that the glycocarotenoids containing galactose were existent in spinach leaves, and in the processes of these carotenoids purification lutein was found to be crystallized easily from cold acetone in considerably good yields. This crystalline lutein had mp. 174-176℃, and E1cm 1% of 2344 at 446 nm. Furthermore, visible absorption spectrum, IR-spectrum, NMR-spectrum and Mass spectrum were also estimated for the identification of it. en-copyright= kn-copyright= en-aut-name=SatoKatsunori en-aut-sei=Sato en-aut-mei=Katsunori kn-aut-name=佐藤勝紀 kn-aut-sei=佐藤 kn-aut-mei=勝紀 aut-affil-num=1 ORCID= en-aut-name=OtaKazuo en-aut-sei=Ota en-aut-mei=Kazuo kn-aut-name=太田和雄 kn-aut-sei=太田 kn-aut-mei=和雄 aut-affil-num=2 ORCID= en-aut-name=KawamotoYasuo en-aut-sei=Kawamoto en-aut-mei=Yasuo kn-aut-name=河本泰生 kn-aut-sei=河本 kn-aut-mei=泰生 aut-affil-num=3 ORCID= affil-num=1 en-affil= kn-affil=岡山大学 affil-num=2 en-affil= kn-affil=鳥取県庁 affil-num=3 en-affil= kn-affil=岡山大学 END