恙虫病病毒と所謂「リケッチア細胞」に関する形態学的研究 第2編 恙虫病病毒が示す染料に対する態度に関する研究

For the staining of Rickettsia tsutsugamushi, several methods have been reported, of which Giemsa's method has been most widely employed. Even this Giemsa's method has been, however, proved not so satisfactory; this method takes a long time, and is not so useful to find the extracellular rickettsiae because of confusing precipitates, and even the rickettsiae unstaible with Giemsa's stain have been reported. Because of these facts, a new useful method is in need nowadays. By employing Giemsa's method, the author already found the significant differences between the shapes of the rickettsiae in serosal cells and those in histiocytes. In this report, the author reinvestigated the hitherto reported several staining methods, and, at the same time, invented some new methods, fuchsin-azure, modified victoria blue and amido-black stainings, by which the morphological strucure of rickettsiae as well as the essential nature of Giemsaunstainable ones was clarified.