タイプ1線毛保有大腸菌のレセプター認識能の多様性の解析

The role of type 1 fimbriae has well been discussed for analyzing the mechanisms of infectious diseases of the urinary tract. In this study, the ability and variety of mannose-receptor recognition in 92 strains of clinically isolated Escherichia coli were examined using various mannose-carriers such as guinea pig red blood cells, Saccharomyces cerevisiae cells and two carriers, latex and liposome. Type 1 fimbriated E. coli showed a variety of reactivity to the receptors on different mannose-carriers. Effects of mannose-carriers and inhibitors in agglutination assays were examined using 48 strongly haemagglutinable strains. In the recognition of Man (1-2) Man-carriers, a high affinity to pNPα-D-mannose was observed in the highly reactive strains to the receptor on liposomes. The MIC ratios of D-mannose to pNPα-D-mannose significantly correlated to the reactivities of these strains to the receptor on the liposome. By the values of MIC ratio, these strains could be classified into two groups ; reactive and non-reactive strains. In the recognition of Man (1-3) Man-carriers, MIC ratios of D-mannose to pNPα-D-mannose differed among more highly reactive strains to Man (1-3) Man-latex, and AUT strains showed stronger reactivity and lower MIC ratio than AUC and ABP strains. The adhesins of type 1 fimbriae were divided into at least 4 type (α, β, γ and δ).