検索結果 13404 件
| タイトル（別表記） | オオムギの組換え型自殖系統、同質遺伝子系統、および倍加半数体系統を用いた発芽時における耐塩性の遺伝解析 |
|---|---|
| フルテキストURL | 004_001_079_088.pdf |
| 著者 | 間野 吉郎| 武田 和義| |
| 抄録 | To determine the relationship between morphological markers and salt tolerance at germination in barley (Hordeum vulgare L.), a total of 125 recombinant inbred (RI) lines of Russia 6×HES 4, a seriesof 70 isogenic (IG) lines for V/ν gene derived from Russia 6×HES 4, and 145 doubled haploid (DH) lines of Leger×CI 9831 were evaluated for their salt tolerance at germination. Comparison between each set of character pairs revealed that the six-rowed type was significantly more tolerant than the two-rowed type in the RI and IG lines of Russia 6×HES 4, annd the two-rowed type and the short haired rachilla type were significantly more tolerant than the six-rowed type and the long haired rachilla type in the DH lines of Leger×CI 9831. On the other hand, no significant difference was observed in salt tolerance such as the smoothness of awn, ear density, and lemma color, r (awn type), l (ear density), and Re-2 (lemma color) genes inherited independent of gene(s) for salt tolerance at germination. |
| キーワード | Doubled haploid lines Hordeum vulgare Isogenic lines Recombinant inbred lines Salt tolerancce |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 79 |
| 終了ページ | 88 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | オオムギのアブラムシ抵抗性とグラミン：EDTA法による解析 |
|---|---|
| フルテキストURL | 004_001_073_078.pdf |
| 著者 | 吉田 英哉| 積木 久明| 河田 和雄| 兼久 勝夫| |
| 抄録 | Young leaves of barley contain a large amount of gramine, which is one of the factors involved in the resistance of barley against aphids. Using stylectomy by laser beam and EDTA-exudate method, we tried to determine if gramine exists in phloem sap which aphids ingest mainly. Phloem sap was not obtained by laser stylectomy using aphids feeding on young leaves. Components of exudates from cut leaves of barley in EDTA solution are known to be very similar to phloem sap. The time course of sucrose and gramine content of EDTA exudates from barley leaves suggested the existence of gramine in phloem sap. |
| キーワード | Barley Resistance to aphids Gramine EDTA Localization |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 73 |
| 終了ページ | 78 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 幼苗期と成熟期のオオムギ系統間における禾穀類アブラムシの密度とグラミン含量の関係 |
|---|---|
| フルテキストURL | 004_001_049_058.pdf |
| 著者 | Moharramipour Saeid| 村田 眞一| 兼久 勝夫| 積木 久明| |
| 抄録 | The relationship between the indole alkaloid gramine concentration and aphid population was examined seedling and maturation stages in 14 barley lines of Hordeum spontaneum and H. unlgare. The density of Schizaphis graminum did not differ significantly with the gramine concentration in the seedling in the greenhouse. However, the population of Rhopalosiphum padi sometimes differed with the seedling. The plant resistance to the natural infestation of cereal aphids was obvious at the heading stage. There was a negative correlation between the high population density of aphids and gramine concentration. The gramine concentration was high in matured resistant resistant lines, especially wild lines, as compared with susceptible lines due to higher biodegradation activity. |
| キーワード | Barley Resistance Gramine Cereal aphids |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 49 |
| 終了ページ | 58 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | トウモロコシに含まれるDIMBOAとアブラムシ抵抗性の関係 |
|---|---|
| フルテキストURL | 004_001_033_042.pdf |
| 著者 | Rustamaki Maqsood A| 兼久 勝夫| 積木 久明| 白神 孝| |
| 抄録 | 2,4-Dihydrox-7-methoxy1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid is considered to be one of the components responsible for the resistance to pest insects in cereal plants. The relationship between concentration of DIMBOA and aphid infestation on 21 corn lines was investigated in 1990 and 1991. DIMBOA was detected in leaves od all corn lines tested, contained larger amounts in young plants and gradually decreased with growth. This property was the same as observed in wheat. A more than ten times difference in DIMBOA concentration was observed in corn lines. However, there was no positive correlation between DIMBOA concentration and aphid density. Most of the lines used had resistance to aphids. Resistance may be built together with other components such as (E)-aconitic acid. |
| キーワード | Apfid Resistant substance Hydroxamic acid DIMBOA Corn |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 33 |
| 終了ページ | 42 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | メダカハネカクシの尾部分分泌物 |
|---|---|
| フルテキストURL | 004_001_025_031.pdf |
| 著者 | 兼久 勝夫| 積木 久明| |
| 抄録 | Rove beetles have a pair of pygidial excretory organs, each group secretes characteristic substances in a peculiar manner. The genus Stenus has a pair of pygidial organs, eversible by immersion in diethyl-ether at both sides of the anus. S. anthoracinus, S. melanarius vercecundus and S. rufescens were collected at ponds and river shores, and S. alienus was collected at tobacco and vegetable fields. Excretory substances were secreted in the diethylether, and identified by gas chromatography and mass spectrometry. Stenusin [N-Ethyl-3-(2-methylbutyl) piperidine], 1,8-cineole, isopiperitenol and 6-Me-5-hepten-2-one were identified. These may probably act as defensive substances against small attacking animals and microorganisms, and act as water suface moving active agents. |
| キーワード | Rove beetles Stenus Excretory secretion Pygidial organ Defensive secretion |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 25 |
| 終了ページ | 31 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | シロイヌナズナ由来過酸化リン脂質グルタチオンペルオキシダーゼ様遺伝子のクローニングと発現 |
|---|---|
| フルテキストURL | 005_002_145_153.pdf |
| 著者 | 杉本 学| 河合 富佐子| |
| 抄録 | A cDNA encoding Arabidopsis purative phosphplipid hydroperoxide gultathione peroxidase (PHGPX) was cloned and sequenced by the reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods. The cDNA comprised 803 bp, and included an open reading frame which encodes a polypeptide of 169 amino acid residues with a molecular mass of 18,600 Da. The deduced amino acid sequence showed homology to plant putative PHGPXs and mammalian PHGPXs. The cloned gene was expressed in Escherichia coli cells to prouce an extra protein, which showed a molecular mass similar to the deduced one. |
| キーワード | Arabidopsis Phospholipid hydroperoxide glutathione peroxidase Nucleotide sequence Gene expression |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 145 |
| 終了ページ | 153 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | イネ幼植物から調整した細胞壁に含まれるペクチン質の性状 |
|---|---|
| フルテキストURL | 005_002_135_144.pdf |
| 著者 | 今野 晴義| 積木 久明| 山崎 良樹| |
| 抄録 | Pectic polysacchasides from the starch-free cell wall preparation of rice (Oryza sativa) shoots have been extracted in sequence with cyclohexane-trans-1,2-diaminetetra-acetate(CDTA)and Na2CO3. The total amount of polysaccharides extracted with the agents was estimated as approximately 1% of the cell walls. The extracted polysaccharides were fractionated by DEAE-Trisacryl M ion-exchange chromatography yielding five fractions, and the monosaccharide composition and molecular mass were constructed from homogalacturonan and rhamnogalacturoanan containing the "hairy" region with galactosyl and arabinosyl side-chains. The solubilized pectic polysaccharides after treatment with two pectolytic enzymes accounted for 0.4～0.6% of the starch-free cell walls. |
| キーワード | Cell wall Oryza sativa Pectic polymer |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 135 |
| 終了ページ | 144 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ナメクジのα-グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 005_002_121_127.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Three forms of α-glucosidase(EC3.2.1.20), designated as Ⅰ, Ⅱ,Ⅲ,have been isoleted from slugs by a procedure including fractionation with ammonium sulfate, Sephacry1 S-200 HR column chromatography, CM-cellulose column chromatography, and pretarative disc gel electrophoresis. The three enzymes readily hydrolyzed maltose and malto-oligosaccharides,but hydrolyzed isomaotose more slowly. α-Glucosidase Ⅲ hydrolyzed soluble starch at a faster rate than maltose, but α-glucosidase Ⅰ hyrolyzed soluble starch more slowly. |
| キーワード | Slug Incilaria bilineata α-glucosidase |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1998 |
| 巻 | 5巻 |
| 号 | 2号 |
| 開始ページ | 121 |
| 終了ページ | 127 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 大麦網斑病抵抗性の選抜効果 |
|---|---|
| フルテキストURL | 003_001_043_053.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | Selection effectiveness for the resistance to net blotch was estimated by using two sets of F2 and F3 populations derived from the crosses between resistant and susceptible parents. In every F2 and F3 population, disease ratings showed a continuous distribution. As many F3 lines with intermediate resistance had a smaller variance and homozygous genotype, the resistance might be controlled by a few genes. The heritabilities of the disease rating were estimated by correlation coefficients and regression coefficients between each F2 plant and the descended F3 lines. Another estimate for heritability was calculated by the selection differential in the F2 plants and genetic gain in the F3 lines. Despite the different level of resistance in the resistant parents of the two crosses, the three kinds of heritabilities estimated were similar and ranged from 0.6 to 0.8. Because of the fewer number of genes controlling the disease resistance and the higher heritabilities, selection in a early generation may be effective for net blotch resistance in barlcy. |
| キーワード | Net blotch Pyrenophora teres Selection Barley Disease resistance |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 43 |
| 終了ページ | 53 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120004537480 |
| タイトル（別表記） | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
|---|---|
| フルテキストURL | 003_001_035_041.pdf |
| 著者 | 侯 福分| 曽 富生| 呉 詩都| 武田 和義| |
| 抄録 | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| キーワード | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 35 |
| 終了ページ | 41 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Ixia から分離された bean yellow mosaic virus |
|---|---|
| フルテキストURL | 004_002_201_213.pdf |
| 著者 | 辻 俊也| 前田 孚憲| 近藤 秀樹| 井上 成信| |
| 抄録 | A strain (Ixia-B) of bean yellow mosaic virus (BYMV) isolated from Ixia hybrida was characterized and compared with other isolates of BYMV and clover yellow vein virus (CYVV). Ixia-B was transmitted by aphids,Myzus presicae in a non-presistent manner and by sap-inoculation to 11 of 46 species in 5 of 10 families tested, and had a similar host range to that of some BYMV isolates, althrough some defferences were detected. Sap from diseased C. quinoa was infective after 10 min heating at 55℃ but not 60℃, after a dilution to 10-3 but not 10-4, and after 2 days but not 4 days at 20℃.The Virus particles were filamentous rods of about 13×820 nm. Ixia-B contaied a single protein species with a molecular weight of 34,000 and a single viral RNA with approximately 9,000 bases. In ultrahtin sections of leaf tissues from infected plants, the virus particles, cylindrical cytoplasmic inclusions and dense bodies were obsserved in the cytoplasm. The antiserum to Ixia-B produced by immunizing a rabbit had a titer of 1/512. A close serological relationship was revealed between Ixia-B and two strains of BYMV from crocus and gladiolus, but no relationship to clover yellow vein virus was found in agar gel diffusion tests. However,Ixia-B could be distinguished from two strains of BYMV by the formation of spurs among them in agar gel and by the differences in the patterns of peptide mapping of coat proteins. From these findings, Ixia-B was identified as a strain of BYMV. |
| キーワード | Ixia hybrida Bean yellow mosaic virus Potyvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 201 |
| 終了ページ | 213 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313395 |
| タイトル（別表記） | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
|---|---|
| フルテキストURL | 004_002_164_174.pdf |
| 著者 | Gara I Wayan| 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| キーワード | Vanda orchid Cymbidium mosaic virus Potexvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 164 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313836 |
| タイトル（別表記） | カクヤリグサ科一年生雑草における数量分類学的研究 |
|---|---|
| フルテキストURL | 002_001_123_134.pdf |
| 著者 | Muhamad Ahmad Chozin| 佐藤 和広| 安田 昭三| |
| 抄録 | Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. |
| キーワード | Cyperus iria Cyperus microiria Cyperus amuricus Numerical taxonomy Speciation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 123 |
| 終了ページ | 134 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313639 |
| タイトル（別表記） | オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較 |
|---|---|
| フルテキストURL | 004_002_137_147.pdf |
| 著者 | 池上 正人| 井上 成信| |
| 抄録 | The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%. |
| キーワード | Tobamovirus Odontoglossum ringspot virus Nucleotide sequence Genome organization |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 137 |
| 終了ページ | 147 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 大麦うどんこ病菌(Erysiphe graminis DC. f. sp. hordei)の地理的分化 |
|---|---|
| フルテキストURL | 002_001_103_110.pdf |
| 著者 | 小西 猛郎| 部田 英雄| |
| 抄録 | Fifteen cultures of barley powdery mildew fungus (Erysiphe graminis DC. f. sp. hordei) isolated in different regions of the world were inoculated to 120 barley varieties. Infection scores varied among the cultures and barley varieties, and their interactions were observed. Principal component analysis of the infection scores revealed that the cultures could be classified into three groups by first and second components (contributing 40.2% and 15.6% of the total variance, respectively), and that two Japanese cultures could be distinguished from the others isolated in Europe and North America. This indicates that the fungus is geographically differentiated in the reaction of the barley varieties to the cultures. Furthermore, East Asian barley varieties differed from European ones in their reactions to the culltures, while barley varieties from regions between East Asia and Europe showed a large genetic diversity in their reactions. |
| キーワード | Barley Powdery mildew Geographic distribution Differentiation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 103 |
| 終了ページ | 110 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | インドネシアのランに発生するウイルスの検索と同定 |
|---|---|
| フルテキストURL | 004_002_109_118.pdf |
| 著者 | 井上 成信| ガラ イ ワヤン| |
| 抄録 | Three viruses, Cymbidium mosaic virsu(CyMV), odontoglossum ringspot virus (ORSV) and an unidentified potyvirus were found in the orchids in Indonesia. CyMV was detected from orchids in 8 genera, namely Aranthera, Calanthe, Cattleya, Cymbidium, Gromatophyllum, Phalaenopsis,Oncidium and Vanda. The virus was widespread in many orchids in Indonesia and was common in Aranthera and Calanthe, thus being an economically important virus in Indonesia.ORSV was also detected in orchids of 5 genera, namely Bulvophyllum,Calanthe, Cattleya,Oncidium and Phalaenopsis. The unidentified potyvirus was found in Aranthera. |
| キーワード | Cymbidium mosaic virus Odontoglossum ringspot virus Unidentified potyvirus Identification Orchids in Indonesia |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 109 |
| 終了ページ | 118 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | 数種植物におけるアンモニウムイオン及び硝酸イオンの吸収速度の比較 |
|---|---|
| フルテキストURL | 003_002_091_103.pdf |
| 著者 | 森次 益三| 河﨑 利夫| 鈴木 孝夫| |
| 抄録 | Absorption rares of ammonium ion(NH4+) and nitrate ion(NO3-) for 24 hours were compared using two absorption solutions, which were a single salt solution of ammonium nitrate (NH4NO3) and a complete nutrient solution containing NH4NO3. Test plants were Oryza sativa (Rice), Hordeum vulgare (Barley), Lactuca sativa (Lettuce), Cucumis sativus (Cucumber), Daucus carota (Carrot), Brassica pekinesis (Chinese cabbage), Spinacia oleracea (Spinach) and Raphanus sativus (Radish). From the absorption characteristics of NH4+ and NO3- between a single salt solution of NH4NO3 and a complete nutrient solution containing NH4NO3, the test plants were classified into goup 1(rice,barley and lettuce), which absorbed NH4+ more rapidly than NO3- in both absorption solutions, group 2(cucumber and carrot), which absorbed NH4+ slightly more than NO3- in the single salt solution of NH4NO3, the tendency of which was reversed in the complete nutrient solution containing NH4NO3, and goup 3(Chinese cabbage,spinach and radish), which absorbed NO3- clealy more than NH4+ in the complete nutrient solution, whereas the absorption of NH4+ or NO3- was almost equal in the single salt solution of NH4+NO3-. The above classisication of plants could be explained by the balance of a repressive or competitive characteristics of NH4+ absorption mainly as sociated with a capacity for absorption of calcium ion(Ca2+) and magnesium ion(Mg2+), and the relative root affinity to NO3- that can be evaluated by the relative absorption of NO3- to mono-phosphate ion(H2PO4-) in plant roots. The group 1 plants are the so-called acid tolerant plants, which appeared to be tolerant to NH4+, and showed a marked pH decreased during the 24 hours of the absorption experiments in those plants. By contrast, the group 3 plant seemed to prefer NO3- and divalent alkaline earth cations to the other nutrients in the complete nutrient solution containing NH4No3, and the pH decrease during each absorption experiment was small. |
| キーワード | Alkaline earth Ammonium Ion balance Nitrate Phosphate |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 2号 |
| 開始ページ | 091 |
| 終了ページ | 103 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | タバコ懸濁培養細胞におけるアルミニウムの二価鉄、銅およびカドミウム毒性に対する影響 |
|---|---|
| フルテキストURL | 002_002_181_190.pdf |
| 著者 | 山本 洋子| 張 藝潔| 小野 寛治| 松本 英明| |
| 抄録 | The effects of aluminum (Al) on the cytotoxicity of ferrous iron (Fe(Ⅱ)), copper(Cu) and cadmium(Cd) were studied. Log-phase cells were treated with either FeSO4,CuSO4, or CdCl2 in the presence or absence of AlCl3(120μM) for 18h at pH 4.0. After the treatment, the viability was determined as relative growth of the metal-treated cells to the untreated control cells during the post-treated culture. A single treated with either Al, Fe(Ⅱ) or Cd did not inhibit the growth at the metal concerntrations up to 300 μM, 200 μM and 500 μM, respectively, whereas the growth was markedly inhibited at 15 μM Cu. Thus,the cells were relatively insensitive to Al, Fe(Ⅱ) and Cd and sensitive to Cu. When cells were treated with both Fe(Ⅱ)(120 μM)and Al(120μM), the growth was significantly inhibited and the cellular contents of both Al and Fe increased synergistically. After the treatment with Cu(0 to 10 μM) with or without Al, the cells grew more vigorously when they were treated in the presence of Al, althrouh the Cu content of the cells were not alterd by Al. The presence of Al during the treatmemt with Cd(0 to 2 μM) had no effect on the degree of growth inhibition by Cd. Thus, Al interacts with the toxicity of Fe(Ⅱ), Cu and Cd in different manners; synergistic with Fe(Ⅱ), antagonistic with Cu and apparently no effeco on Cd. |
| キーワード | Aluminum Antagonistic Nicotiana tabacum Suspension culture Synergistic |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 2号 |
| 開始ページ | 181 |
| 終了ページ | 190 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ゼニゴケ葉緑体遺伝子と相同性を持つラン藻 Synechocystis PCC6803株のORF326、frxC およびORF469を標的にした変異の導入 |
|---|---|
| フルテキストURL | 002_002_167_180.pdf |
| 著者 | 小倉 豊| 竹村 美保| 小田 賢司| 大山 莞爾| |
| 抄録 | ORF326, frxC and ORF469 of a transfomable cynobacterium, Synechcystis PCC6803, have sequence similarity with ORF465 on the choroplast genome of a livewort, Marchantia polymorpha, respectively. To elucidate their functions,targeted mutagenesis was performed by transformation with clened DNA in which the ORF was disrupted by insertion of a kanamycin resistancen gene cassette.Streak-purifications of a single colony of each transformant were repeatde to segregate homozygous mutants for disrupted copies, because Synechocystis PCC6803 was reported to have approximately 10 chromosomal DNA copies. Southern blot analysis revealed that mutants for ORF326 had not only disrupted ORF326 copies but also wild type ORF326 copies. This suggests that ORF326 is indispensable for growth under the mixotrophic growth condition used. However, mutants for frxC and mutants for ORF469 had only mutated copies, indicating that they dispensable for growth. Growth and chlorophyll a content of an ORF469-disrupted mutant were compared and chlorophyll a content of an ORF469-disrupted mutant were compared to those of wild type under mixotrophic growth condition, but no significant difference was detected. This indicates that ORF469 is required for neither normal growth nor chlorophyll biosynthesis under thie condition. |
| キーワード | Cyanobacteria Gene disruption Synechocystis PCC6803 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 2号 |
| 開始ページ | 167 |
| 終了ページ | 180 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 001_002_159_166.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| キーワード | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 159 |
| 終了ページ | 166 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |