検索結果 1285 件
| タイトル（別表記） | ホウレンソウ種子に存在するα-グルコシダーゼの分子多型変化 |
|---|---|
| フルテキストURL | 005_001_001_009.pdf |
| 著者 | 古井 聡| 杉本 学| 鈴木 幸雄| |
| 抄録 | Two molecular forms of α-glucosidase were isolated from spinach seeds after storage at 4℃ by CM-cellulose column chromatography and gel filtration. The molecular masses of α-glucosidase A and B were 78 kDa and 82 kDa by SDS-PAGE, and 62 kDa and 70 kDa by gel filtration, respectively. α-Glucosidase A had high activity not only toward maltooligosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 65℃ for 20 min. On the other hand, α-glucosidase B had high activity toward maltooligosaccharides but faint activity toward α-glucans. The optimum pH was 5.0 and no activity was found after incubation at 65℃ for 20 min. The enzymatic and immunological properties of α-glucosidase A and B were similar to those of α-glucosidase. Ⅰor Ⅱ, and α-glucosidase Ⅲ or Ⅳ, isolated from spinach seeds without 4℃ storage, respectively. These findings suggest that the α-glucosidase in spinach seeds is modified to be two molecular forms. |
| キーワード | Spinach α-Glucosidase Multiple molecular forms |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1997 |
| 巻 | 5巻 |
| 号 | 1号 |
| 開始ページ | 1 |
| 終了ページ | 9 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | QTL Mapping for Water Sensitivity in Barley Seeds |
|---|---|
| フルテキストURL | 006_001_021_028.pdf |
| 著者 | 岩佐 友彦| 高橋 秀和| 武田 和義| |
| 抄録 | Sensitivity to water condition during germination is an important character in malting barley because it affects malt poduction. Water sensitivity in malting barley is defined as the defference in germination percentage between the seeds in standard and excess-water conditions. Usually standard and excess-water condition means 4 and 8 ml water in a 90 mm perti dish, respectively. Takeda and Fukuyama (1983) examined more than 4,000 barley accessions and found 0～100% water sensitivity. This means complete to no suppression of germination by an excess-water condition. In this study, quantiative trait loci (QTL) analysis was applied to analize the inheritance of water sensitivity. Two sets of doubled haploid (DH) populations derived from Steptoe×Morex (S/M) and Harrington×TR306 (H/T) crosses,were grown in the experiment field of the Research Institute for Bioresources, Okayama University. Bulbosum method was adapted to develop DH lines (Chen and Hayes 1989). Water sensitivities of 150 (S/M) and (H/T) lines and their parents were evaluated using 50 seeds each and the germination test (25℃, 4 days) was repeated four times. Water sensitivity showed a large variation (Fig.1) and the heritability of the trait was 0.78 and 0.72 in S/M and H/T, respectively, indicating that about three-quarters of the phenotypic variance was genetic in origin. Three (S/M) and one (H/T) significant QTL were detected and 23% (S/M) and 25% (H/T) of the phenotypic variance was explained by the QTL. These QTL were located on chromosomes 2H,3H,6H and 7H (Table 1, Fig. 2). QTL relating to germination traits were lacated near the QTL controlling water sensitivity (Fig. 3). Interaction among the QTL was small (Table 2). Marker-assisted selection was effective (Table 3). |
| キーワード | barley water sensitivity QTL analysis |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1999 |
| 巻 | 6巻 |
| 号 | 1号 |
| 開始ページ | 21 |
| 終了ページ | 28 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | オオムギの組換え型自殖系統、同質遺伝子系統、および倍加半数体系統を用いた発芽時における耐塩性の遺伝解析 |
|---|---|
| フルテキストURL | 004_001_079_088.pdf |
| 著者 | 間野 吉郎| 武田 和義| |
| 抄録 | To determine the relationship between morphological markers and salt tolerance at germination in barley (Hordeum vulgare L.), a total of 125 recombinant inbred (RI) lines of Russia 6×HES 4, a seriesof 70 isogenic (IG) lines for V/ν gene derived from Russia 6×HES 4, and 145 doubled haploid (DH) lines of Leger×CI 9831 were evaluated for their salt tolerance at germination. Comparison between each set of character pairs revealed that the six-rowed type was significantly more tolerant than the two-rowed type in the RI and IG lines of Russia 6×HES 4, annd the two-rowed type and the short haired rachilla type were significantly more tolerant than the six-rowed type and the long haired rachilla type in the DH lines of Leger×CI 9831. On the other hand, no significant difference was observed in salt tolerance such as the smoothness of awn, ear density, and lemma color, r (awn type), l (ear density), and Re-2 (lemma color) genes inherited independent of gene(s) for salt tolerance at germination. |
| キーワード | Doubled haploid lines Hordeum vulgare Isogenic lines Recombinant inbred lines Salt tolerancce |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 1号 |
| 開始ページ | 79 |
| 終了ページ | 88 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Purification and Characterization of α-Glucosidases from Spinach Seeds |
|---|---|
| フルテキストURL | 004_002_239_252.pdf |
| 著者 | 杉本 学| 古井 聡| 鈴木 幸雄| |
| 抄録 | Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 78,78,82 and 82kDa by SDS-PAGE, and 62,62,190,and 70kDa by gel filtration, respectively. α-Glucosidases Ⅰand Ⅱ showed similar enzymatic properties. The Km for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only towards malto-oligosaccharides but also towards α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 71℃ for 20 min. On the other hand, α-glucosidases Ⅲ and Ⅳ showed similar enzymatic propreties. The Km for maltose was 3-4 times lower than for solble starch, and they had high activity toward malto-oligosaccharides but faint activity towards α-glucnas. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20 min. However, anti-α-glucosidase Ⅲ serum precipitated specifically with α-glucosidase Ⅲ. |
| キーワード | α-Glucosidase Spinach Seed Spinacia oleracea L. Molecular form |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 239 |
| 終了ページ | 252 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Some Properties of Azuki Bean Mosaic Virus Isolated from Soybean Plants in Ibaraki Prefecture |
|---|---|
| フルテキストURL | 004_002_215_227.pdf |
| 著者 | 高橋 幸吉| 井上 成信| 前田 孚憲| 光畑 興二| 千田 茂樹| 高橋 義行| |
| 抄録 | Azuki bean mosaic virus (ABMV) was isolated from Glycine max showing mild mosaic and stunt on the leaves, collected in Ibaraki-Prefecture, in July, 1989. ABMV was transmitted by sap-inoculation to 8 species systemically and 14 species locally out of 33 species in 8 families and by aphids, Aulacorthum solani and Myzus persicae in a non-persistent manner.Seed transmission was recognized in 0.8% of the seeds of soybean cv."Iwate-wase-kurome" inoculated at seeding stage. The soybean plant infected with ABMV produced mottled seeds, which were radical- or saddle-like. The virus particles were flexuous rods, bout 750 nm in length. In ultrathin virus-infected tissues, cytoplasmic inclusions containing pinwheels and scrolls were observed in the cytoplasms. In DAS-ELISA, the virus reacted strongly with antiserum to ABMV. The weight of plants and seed of two soybean cv."Okuhara 1" and "Iwate-wase-kurome" inoculated with ABMV at the seedling stage was about 40～50% less than that of healthy plants. |
| キーワード | Potyvirus Azuki bean mosaic virus Glycine max Properties |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 215 |
| 終了ページ | 227 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | ダイズ種子の冠水抵抗性の品種間差とダイアレル分析 |
|---|---|
| フルテキストURL | 003_001_035_041.pdf |
| 著者 | 侯 福分| 曽 富生| 呉 詩都| 武田 和義| |
| 抄録 | Seven hundred and thirty varieties of soybean from different sources were evaluated for seed flooding tolerance by the seed germination test after a 4-day soaking at 25℃. The inheritance of seed flooding tolerance was also analyzed with a 6 x diallel cross. A large variation in seed flooding tolerance existed in the soybean germplasm as reflected in the germination rate which ranged from 0 to 100%. Most of the varieties tested were sensitive to seed flooding, and only 4% of the tested varieties showed a high tolerance (germination rate >90%). Seed flooding tolerance was controlled by both additive and dominance genes and a small number of effective factors and high narrow sense heritability in diallel analysis indicate that the selection for tolerance can effectively be done in early generations. |
| キーワード | Glycine max Seed flooding tolerance Diallel analysis Germination Varietal difference |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1995 |
| 巻 | 3巻 |
| 号 | 1号 |
| 開始ページ | 35 |
| 終了ページ | 41 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | バンダから分離されたCymubidium Mosaic Virusの諸性質 |
|---|---|
| フルテキストURL | 004_002_164_174.pdf |
| 著者 | Gara I Wayan| 近藤 秀樹| 前田 孚憲| 光畑 興二| 井上 成信| |
| 抄録 | A virus causing necrotic spots and necrotic flecks on the leaves of Vanda orchids in Japan was identified as cymbidium mosaic virus(Cymv) on the basis of host range,stabilly in crude sap, particle morphology, serological test and physico-chemical properties. The virus was transmitted by sap inoculation to 12 of 57 species in 6 of 12 families tested, but not by aphid Mizus persicae or through seeds. Systemic infection occurred in all Orchidaceae plants tested and only one in non-orchidaceae (Sesamum indicum). In Tetragonia expansa sap, the infective at a dilution of 10-5 but not at 10-6, after heating at 65℃ for 10 min, and was still active after 1 month aging in vitro. Flexuous rod particles, c. 475×13nm, were observed.In ultrahtin sections of leaf tissues from diseased plants, virus particles were found to aggregate in the cytoplasm. The molecular weight of the protein submit and RNA determined by gel electrophoresis, was 27.8×103 and 2.2×106, respectively. Double-stranded RNAs with estimated molecular weight of 5.4×106, 4.0×106, 3.6×106 and 3.0×106 were isolated from infected plants. |
| キーワード | Vanda orchid Cymbidium mosaic virus Potexvirus |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1996 |
| 巻 | 4巻 |
| 号 | 2号 |
| 開始ページ | 164 |
| 終了ページ | 174 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313836 |
| タイトル（別表記） | カクヤリグサ科一年生雑草における数量分類学的研究 |
|---|---|
| フルテキストURL | 002_001_123_134.pdf |
| 著者 | Muhamad Ahmad Chozin| 佐藤 和広| 安田 昭三| |
| 抄録 | Three species of Cyperaceous weeds, Cyperus iria (12 strains), C. microiria(12 strains) and C. amuricus (6 strains), were collected from different sites of Okayama, Tottori and Tokyo prefectures, and various morphological characters, biomass and seed production were observed on the plants which were cultivated at Kurashiki. The analysis of variance showed a significant difference among the strains in each character. However, the species overlapped with each other in most morphological characters. Prinipal component analysis on the 21 characters showed that 83% of the total variation could be explained by the first three components: the first component (37%) was regarded as factors concerning spikelet and seed production; the second component (28%) was regarded as factors concerned the size of vegetative parts; the third component (18%) was largely affected by seed weight and floret density. Scatter diagram on the first and third principal components showed that the 30 strains of three species divided into three groups, and strains in each group correspond to the three species without exception. Based on the second and third principal components, strains of C. microiria were further divided into three sub-groups according to size of vegetative parts. Using the cluster analysis, 30 strains of these species were divided into four large clusters; the first was composed of C. amuricus strains, the second was of three strains of C. microiria, the third included the remaining strains of C. microiria, and the last cluster was composed of C. iria strains. It may be concluded that C. microiria is composed of two or three ecotypes which are different in morphological and reproductive traits. |
| キーワード | Cyperus iria Cyperus microiria Cyperus amuricus Numerical taxonomy Speciation |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1994 |
| 巻 | 2巻 |
| 号 | 1号 |
| 開始ページ | 123 |
| 終了ページ | 134 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313639 |
| タイトル（別表記） | Establishment of a Seedling Test for Resistance to Net Blotch in Barley and a Search for Resistant Varieties |
|---|---|
| フルテキストURL | 001_001_075_090.pdf |
| 著者 | 佐藤 和広| 武田 和義| |
| 抄録 | 大麦網斑病は糸状菌の1種であるPyrenophora teres Drechs.の感染によって葉身、葉鞘等に網目状の病斑を生じ、子実の登熟低下によって減収する共に、ビールオオムギにおいては醸造品質であるエキス分が低下する重要病害である。本病害は世界各地のオオムギ栽培地帯のうち主として温暖・湿潤な地域に分布しており（Shipton et al.1973）、近年、連作や灌漑によって被害が増大しつつある（Mathre 1982）。我国においては従来からその存在が確認されていたものの、登熟後期の活性の衰えた葉に生じる病害として重要性は認識されていなかった。しかし、最近、北海道、鳥取県、鹿児島県などのビールオオムギ栽培地帯で局所的な激発事例が確認されている。（佐藤、未発表）。本病害に対する防除法としては種子消毒ならびに殺菌剤の茎葉撒布が有効であるが、その効果は完全ではない。また、茎葉撒布はコストが高く、環境汚染の問題もあるので、最も有効で経済的かつ安全な防除法は抵抗性品種を栽培することと言っても良い。従来、本病害の積極的な抵抗性育種は行われていなかったが、最近は抵抗性を有する品種も育成されている（Metcalfe 1987）。抵抗性品種を育成するためには、遺伝資源ならびに雑種後代を効率よく評価、選抜するための検定方法を確立しなければならない。本病抵抗性の検定方法としては幼苗検定法、圃場検定法が考案されて広く用いられているが（Buchannon and McDonald 1965, Holtmeyer and Webster 1981）、環境条件の変化によって抵抗性が変動する事例が報告されているので（Khan and Boyd 1970, Tekauz 1986）、抵抗性を確実に評価するための安定した検定条件を設定する必要がある。抵抗性に関する遺伝資源についてはSchaller and Wiebe （1952）、Dessouki et al.（1965）およびBuchannon and McDonald （1965）等がそれぞれ数千品種を評価し、中国東北部、トルコおよびエチオピアなどに抵抗性の遺伝資源が豊富であること報告している。それらの品種のいくつかについては、抵抗性の遺伝子分析が行われており（Bockelman et al. 1977, Davis et al. 1990）、本病抵抗性育種の交配親として使用されている（Tekazu and Buchannon 1977, Moseman and Smith 1985）。岡山大学資源生物科学研究所大麦系統保存施設は世界的にも貴重なと東アジアの遺伝資源をはじめ五千余の保存品種を有するが、著者らは大麦網斑病の幼苗検定法を確立し、これらの品種の抵抗性を評価したので報告する。 |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1992 |
| 巻 | 1巻 |
| 号 | 1号 |
| 開始ページ | 75 |
| 終了ページ | 90 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313461 |
| タイトル（別表記） | テンサイ培養細胞の細胞壁結合α―グルコシダーゼの精製と性質 |
|---|---|
| フルテキストURL | 001_002_159_166.pdf |
| 著者 | 山崎 良樹| 今野 晴義| |
| 抄録 | Wall bound α-glucosidase (EC 3.2.1.20) has been solubilized from suspension-cultured sugar-beet cells with Sumyzyme C and Pectolyase Y-23 and purified by a procedure including fractionation with ammonium sulfate, Sephacry S-200 HR column chromatography, and CM-cellulose colum chromatography. The enzyme readily hydrolyzed maltose, nigerose, malto-oligosaccharides, and soluble starch, but hydrolyzde isomaotose more slowly. The enzyme hydrolyzed malto-oligosaccharides and soluble starch at a faster rate than maltose. The wall-bound α-glucosidase from sugar-beet cells is different from the enzymes extracted from the cells and seeds in substrate spesificity. |
| キーワード | Beta vulgaris L. cv. Tsukisappu Sugar-beet Wall-bound enzyme α-Glucosidase Protoplast |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 159 |
| 終了ページ | 166 |
| ISSN | 0916-930X |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | The Effect on Germination of Some Species of the Genus Echinochloa and Rice Seeds with Various Osmotic Pressure of Salt, Sugar, and PEG Solutions |
|---|---|
| フルテキストURL | 001_002_131_136.pdf |
| 著者 | 西 克久| |
| 抄録 | To investigate the differences and mechanism of germination among the seeds of some species of genus Echinochloa and rice, the experiments were conducted in solutions of salt, sugar, and PEG(poly-ethylene glycol) under a constant or alternating temperature in the light or the dark. The seeds of Echinochloa oryzicola Vasing., Ehinochloa crus-galli Beauv.var. formosensis Ohwi, Echinochloa crus-galli Beauv.var.crus-galli(awnless and awned type)and Oryza sativa L.(Akebono) were used. In 7000hPa, germination of rice,E. oryzicola and three varieties of E.crus-galli in all the solutions remarkedly decreased except for the case of E.crus-galli in the solution of sale on alternating temperature. The germination of rice and E.oryzicola was secreased markedly, and more markedly in a light condition than in a dark. In the salt solution in 7000hPa, the decreasing of germination of E.crus-galli was low. This species is distributed and grows in the upland and paddy field, while E.oryzicola and rice grow only in the paddy field. The decreade in germination of Echinochloa species with osmotic pressure seemed to be recoverde with salt or sugar solutions under alternating temperature, but the recovery of rice was not seen. In 700hPa or less, the distinct differences of germination were not seen among these plants species. |
| キーワード | Genus Echinochloa Germination Osmotic pressure |
| 出版物タイトル | 岡山大学資源生物科学研究所報告 |
| 発行日 | 1993 |
| 巻 | 1巻 |
| 号 | 2号 |
| 開始ページ | 131 |
| 終了ページ | 136 |
| ISSN | 0916-930X |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| 著者 | 後藤 丹十郎| |
|---|---|
| 発行日 | 1995-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 84巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 後藤 丹十郎| 山登 千恵| 景山 詳弘| 小西 国義| |
|---|---|
| 発行日 | 1996-02-01 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 85巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 木村 吉伸| 高木 茂明| |
|---|---|
| 発行日 | 1998-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 87巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | ウィドド ウィナルソ ドラジャド| 岡本 五郎| 平野 健| |
|---|---|
| 発行日 | 1999-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 88巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 山井 雅文| 木村 吉信| |
|---|---|
| 発行日 | 2000-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 89巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 木村 吉伸| 鈴木 将史| |
|---|---|
| 発行日 | 2001-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 90巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 畑 直樹| |
|---|---|
| 発行日 | 2006-03-24 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 豊田 和弘| Hikichi, Yasufumi| Takeuchi, Shigeharu| Okumura, Ako| Nasu, Yoshiko| Okuno, Tetsuro| Suzuki, Kazumi| |
|---|---|
| 発行日 | 2004-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 93巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |
| 著者 | 福田 文夫| 近阪 昌之| 久保田 尚浩| |
|---|---|
| 発行日 | 2006-02 |
| 出版物タイトル | 岡山大学農学部学術報告 |
| 巻 | 95巻 |
| 号 | 1号 |
| 資料タイプ | 紀要論文 |