ID | 64853 |
フルテキストURL | |
著者 |
Chijimatsu, Ryota
Okayama University Hospital, Center for Comprehensive Genomic Medicine
Takeda, Taiga
The University of Tokyo, Bone and Cartilage Regenerative Medicine, Graduate School of Medicine
Tsuji, Shinsaku
CPC Corporation
Sasaki, Kohei
Japan Vilene Company, Ltd., Central Research Laboratory
Kato, Koichi
Japan Vilene Company, Ltd., Central Research Laboratory
Kojima, Rie
Japan Vilene Company, Ltd., Central Research Laboratory
Michihata, Noriko
Japan Vilene Company, Ltd., Central Research Laboratory
Tsubaki, Toshiya
The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
Matui, Aya
CPC Corporation
Watanabe, Miharu
CPC Corporation
Tanaka, Sakae
The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
Saito, Taku
The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
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抄録 | Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues.
Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion. Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties. Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation. |
キーワード | Adipose stem cells
Explant culture
Nonwovens
Hydroxyapatite
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発行日 | 2022-12
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出版物タイトル |
Regenerative Therapy
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巻 | 21巻
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出版者 | Elsevier B.V.
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開始ページ | 52
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終了ページ | 61
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ISSN | 23523204
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資料タイプ |
学術雑誌論文
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言語 |
英語
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OAI-PMH Set |
岡山大学
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著作権者 | © 2022 The Japanese Society for Regenerative Medicine.
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論文のバージョン | publisher
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PubMed ID | |
DOI | |
Web of Science KeyUT | |
関連URL | isVersionOf https://doi.org/10.1016/j.reth.2022.05.009
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ライセンス | http://creativecommons.org/licenses/by-nc-nd/4.0/
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