このエントリーをはてなブックマークに追加


ID 64853
フルテキストURL
fulltext.pdf 5.49 MB
著者
Chijimatsu, Ryota Okayama University Hospital, Center for Comprehensive Genomic Medicine
Takeda, Taiga The University of Tokyo, Bone and Cartilage Regenerative Medicine, Graduate School of Medicine
Tsuji, Shinsaku CPC Corporation
Sasaki, Kohei Japan Vilene Company, Ltd., Central Research Laboratory
Kato, Koichi Japan Vilene Company, Ltd., Central Research Laboratory
Kojima, Rie Japan Vilene Company, Ltd., Central Research Laboratory
Michihata, Noriko Japan Vilene Company, Ltd., Central Research Laboratory
Tsubaki, Toshiya The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
Matui, Aya CPC Corporation
Watanabe, Miharu CPC Corporation
Tanaka, Sakae The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
Saito, Taku The University of Tokyo, Sensory and Motor System Medicine, Graduate School of Medicine
抄録
Adipose-derived stem cells (ASCs) are an attractive cell source for cell therapy. Despite the increasing number of clinical applications, the methodology for ASC isolation is not optimized for every individual. In this study, we developed an effective material to stabilize explant cultures from small-fragment adipose tissues.
Methods: Polypropylene/polyethylene nonwoven sheets were coated with hydroxyapatite (HA) particles. Adipose fragments were then placed on these sheets, and their ability to trap tissue was monitored during explant culture. The yield and properties of the cells were compared to those of cells isolated by conventional collagenase digestion.
Results: Hydroxyapatite-coated nonwovens immediately trapped adipose fragments when placed on the sheets. The adhesion was stable even in culture media, leading to cell migration and proliferation from the tissue along with the nonwoven fibers. A higher fiber density further enhanced cell growth. Although cells on nonwoven explants could not be fully collected with cell dissociation enzymes, the cell yield was significantly higher than that of conventional monolayer culture without impacting stem cell properties.
Conclusions: Hydroxyapatite-coated nonwovens are useful for the effective primary explant culture of connective tissues without enzymatic cell dissociation.
キーワード
Adipose stem cells
Explant culture
Nonwovens
Hydroxyapatite
発行日
2022-12
出版物タイトル
Regenerative Therapy
21巻
出版者
Elsevier B.V.
開始ページ
52
終了ページ
61
ISSN
23523204
資料タイプ
学術雑誌論文
言語
英語
OAI-PMH Set
岡山大学
著作権者
© 2022 The Japanese Society for Regenerative Medicine.
論文のバージョン
publisher
PubMed ID
DOI
Web of Science KeyUT
関連URL
isVersionOf https://doi.org/10.1016/j.reth.2022.05.009
ライセンス
http://creativecommons.org/licenses/by-nc-nd/4.0/