| JaLCDOI |
10.18926/AMO/49042
|
| フルテキストURL |
66_6_461.pdf
|
| 著者 |
Koike, Kazuko|
Takaki, Akinobu|
Kato, Nobuyuki|
Ouchida, Mamoru|
Kanzaki, Hirotaka|
Yasunaka, Tetsuya|
Shiraha, Hidenori|
Miyake, Yasuhiro|
Yamamoto, Kazuhide|
|
| 抄録 |
Hepatitis C virus (HCV) infection induces several changes in hepatocytes, such as oxidative stress, steatosis, and hepatocarcinogenesis. Although considerable progress has been made during recent years, the mechanisms underlying these functions remain unclear. We employed proteomic techniques in HCV replicon-harboring cells to determine the effects of HCV replication on host-cell protein expression. We examined two-dimensional electrophoresis (2-DE) and mass spectrometry to compare and identify differentially expressed proteins between HCV subgenomic replicon-harboring cells and their “cured” cells. One of the identified proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Full-length HCV genome RNA replicating and cured cells were also assessed using ELISA. Replicon-harboring cells showed higher expression of retinal dehydrogenase 1 (RALDH-1), which converts retinol to retinoic acid, and the cured cells showed higher expression of retinol-binding protein (RBP), which transports retinol from the liver to target tissues. The alteration in RBP expression was also confirmed by ELISA and Western blot analysis. We conclude that protein expression profiling demonstrated that HCV replicon eradication affected retinol-related protein expression.
|
| キーワード |
hepatitis C virus
retinol-binding protein
|
| Amo Type |
Original Article
|
| 出版物タイトル |
Acta Medica Okayama
|
| 発行日 |
2012-12
|
| 巻 |
66巻
|
| 号 |
6号
|
| 出版者 |
Okayama University Medical School
|
| 開始ページ |
461
|
| 終了ページ |
468
|
| ISSN |
0386-300X
|
| NCID |
AA00508441
|
| 資料タイプ |
学術雑誌論文
|
| 言語 |
英語
|
| 著作権者 |
CopyrightⒸ 2012 by Okayama University Medical School
|
| 論文のバージョン |
publisher
|
| 査読 |
有り
|
| PubMed ID |
23254580
|
| Web of Science KeyUT |
000312966100005
|
