検索結果 9436 件
| JaLCDOI | 10.18926/11861 |
|---|---|
| タイトル（別表記） | The State of the Applicants to Our Midwife Training |
| フルテキストURL | 004_011_017.pdf |
| 著者 | 白井 喜代子| 合田 典子| |
| 抄録 | Nursing students taking the entrance examination for our midwifery training program in the academic year 1992-1993 were evaluated with regard to what motivated them to apply to our program and their plans after graduation. The results were as follows : 1. The wish of the nursing students themselves was the decisive factor in their decision to apply. 2. Our investigation revealed that 10% of the nursing students decided to take up midwifery after graduation from nursing school; 25% decided to do so before their entrance to nursing school : and 65% made up their minds while they were still at nursing school. 3. The applicants planned to work as professional midwives for a long time after their completion of the midwifery training program. 4. Junior college graduates, after receiving authorization from the National Institution of Academic Degrees, were motivated to apply to our midwifery training program for the explicit purpose of acquiring a bachelor's degree. |
| キーワード | 助産婦課程受験者 進学動機 進路希望 |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1994-01-31 |
| 巻 | 4巻 |
| 開始ページ | 11 |
| 終了ページ | 17 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002314047 |
| 著者 | プープイント ハン| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11852 |
|---|---|
| タイトル（別表記） | Delivery Assistance Techniques |
| フルテキストURL | 004_001_009.pdf |
| 著者 | 合田 典子| 岡﨑 愉加| 白井 喜代子| |
| 抄録 | The delivery assistance laboratory is one of the most important part of the professional curriculum in the training program for midwives. The educational significance of 10 cases of delivery assistance was reviewed. The technical standards of the students improved with accumulation of experience in the assistance of deliveries. Evaluation of the methods in which the students were assessed with regard to technical improvement in delivery assistance was performed. Of the total number of 136 students of midwifery registered at our school in the past 7years, 50 who were able to assist successively in 3-10 cases of normal childbirth were evaluated in this study with regard to their acquisition of skills needed to assist in deliveries. Fourteen technical items related to the assistance of delivery in its second and third stages were assessed. An evaluation standard consisting of 3 grades, namely "able to perform", "somehow able to perform", and "not able to perform" regarding the above mentioned 14 technical items were used to assess the mastery of delivery assistance techniques by the students. Our results showed that as the students accumulated experience in delivery assistance, self-assessment ratings of "able to perform" increased on the average. The accumulation of skill concerning the management of the neonate immediately after birth, the instruction of patients regarding the application of abdominal pressure, and the assistance during crowning was specially evident. In contrast, although self-assessment ratings of "able to perform" with regard to "assistance of the mother immediately after giving birth" and "assistance immediately after the delivery of the fetal head" did increase, its rate of increase was small. |
| キーワード | 助産婦教育 助産技術 実習評価 分娩介助実習 |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1994-01-31 |
| 巻 | 4巻 |
| 開始ページ | 1 |
| 終了ページ | 9 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313852 |
| 著者 | 岡山大学農学部附属山陽圏フィールド科学センター| |
|---|---|
| 発行日 | 2005-12-01 |
| 出版物タイトル | 岡山大学農学部センター報告 |
| 巻 | 27巻 |
| 資料タイプ | その他 |
| JaLCDOI | 10.18926/11849 |
|---|---|
| タイトル（別表記） | Physical Image Quality of Rapid Processing Films |
| フルテキストURL | 003_099_104.pdf |
| 著者 | 吉田 彰| 本田 貢| 中村 伸枝| 有岡 匡| 小橋 利美| 竹内 知行| 三上 泰隆| 杉田 勝彦| 平木 祥夫| |
| 抄録 | The imaging properties of Konica "new" SR series films and "old" MG-SR series films for rapid processing were measured in combination with green-emitting intensifying screens. The measured image quality prarameters include: characteristic curve, modulation transfer function (MTF) and Wiener spectrum. The relative speeds of the new SR series systems were slower than those of the old MG-SR series systems from 10 to 25%. The MTF's of the new systems were generally comparable to those of the old systems, except that SR-G system was improved with an increase of approximately 10%. In the Wiener spectrum measurement, however, the new systems showed from 10 to 35% decrease in low-frequency noise. |
| キーワード | Rapid Processing Films Image Quality Characteristic Curve MTF Wiener Spectrum |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 99 |
| 終了ページ | 104 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002314046 |
| JaLCDOI | 10.18926/11845 |
|---|---|
| タイトル（別表記） | A Tendency of the Bacterial Food-Poisoning from 1981 to 1991 |
| フルテキストURL | 003_087_091.pdf |
| 著者 | 赤塚 和也| |
| 抄録 | From the viewpoint of the tendency by the bacterial food-poisoning from 1981 to 1991, it seems that the tendency is not relatively changed in comparison with the data before 1981. However, a few changes are appeared in pathogenic substances, preparing facilities, and serving places, respectively. Namely, the tendency to be just little decrease of the incident rate of Vibrio parahaemolyticus and Staphylococcus aureus on pathogenic substances is appeared in 10 years. Furthermore, on the preparing facilities and serving places, it seems that restaurants and caterers are characterized by the increase of the incident rate of bacterial food poisonings togather with the increases of food service industries. |
| キーワード | 食中毒 Vibrio parahaemolyticus Staphylococcus aureus Esherichia coli 魚介類 |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 87 |
| 終了ページ | 91 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313930 |
| JaLCDOI | 10.18926/11840 |
|---|---|
| タイトル（別表記） | The Utopia of Western Mind and Experimental Science New Atlantis (F.Bacon, 1627) |
| フルテキストURL | 003_067_074.pdf |
| 著者 | 武田 光史| |
| 抄録 | TO THE READER by Bacon's secretary William Rawley when he published New Atlantis after Bacon's death is quoted here as a substitute for the synopsis of this paper : This Fable my Lord devised, to the end that he might exhibit therein, a model or description of a College, instituted for the interpreting of Nature, and the producing of great and marvellous works for the benefit of man ; under the name of Salomon's House, or the College of the Six Days' Works. And even so far his Lordship hath proceded, as to finish that part. Certainly, the model is more vast, and high, than can possibly be imitated in all things ; Notwithstanding most things therein are within men's power to effect････ |
| キーワード | Renaissance Utopia Francis Bacon New Atlantis Experimental Science |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 67 |
| 終了ページ | 74 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313706 |
| 著者 | 三木 厚| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11811 |
|---|---|
| タイトル（別表記） | The health and daily-life problem of the aged at homes for three months after discharge from the hospital |
| フルテキストURL | 007_2_165_170.pdf |
| 著者 | 猪下 光| 高田 節子| 近藤 益子| 太田 にわ| 池田 敏子| 中西 代志子| 小島 操子| |
| 抄録 | 今後の継続看護・在宅看護を考えてゆく際の参考にするために、医療施設から自宅へ退院した高齢患者が3カ月経過後に、どのような健康上や日常生活上の問題点をもち、家庭や地域でどのような援助や支援を受けて生活しているかについて報告をする。総合病院に入院していた70歳以上の高齢者で自宅に退院する患者92名のうち、退院後3カ月経過後の時点で回答を得た70名（76.0％）について分析を行った。病状については約8割の者は特に問題を持っていない、しかし約2割の者は病状に悪化が見られた。現在困っていることは「健康上の問題」とした者は約3割であった。日常生活の自立状況では「仕事もできる」とする者が退院時の18.6％より、3カ月後は40.0％に増加しており、「生きがいがかなりある」とする者は退院時の34.3％より、3カ月後は45.7％に増加していた。しかし、回答が得られなかった22名（24.0％）の高齢者は、より重要な問題を包含している可能性があり、追跡をする必要がある。 |
| キーワード | 高齢患者 (the aged) 自宅への退院 (pations at home) 3カ月経過後の問題 (problems) 在宅看護 (home treatment) 継続看護 (continuing-nursing) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1997-01-31 |
| 巻 | 7巻 |
| 号 | 2号 |
| 開始ページ | 165 |
| 終了ページ | 170 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313404 |
| JaLCDOI | 10.18926/11808 |
|---|---|
| タイトル（別表記） | RESOLUTION PROPERTIES OF CRT MONITORS |
| フルテキストURL | 002_081_088.pdf |
| 著者 | 竹内 知行| 吉田 彰| 東 義晴| 杉田 勝彦| 中村 伸枝| 岸 幹二| 平木 祥夫| |
| 抄録 | We have developed a new technique for measuring the modulation transfer function (MTF) of a cathode ray tube (CRT) monitor. The "nominal" MTF was measured by displaying a line image of a width of one pixel on the CRT monitor using the pattern generator. We also measured the effective pixel width. In order to obtain the "actual" MTF, the nominal MTF was divided by the sinc function which is the Fourier transform of the effective pixel width. The results indicate that the corrected MTF converges to the constant value as the effective pixel width is narrower. Thus, the actual MTFs were determined and compared in the horizontal direction for two CRT monitors. |
| キーワード | CRT Monitor Resolution Effective Pixel Width Modulation Transfer Function (MTF) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1992-02-05 |
| 巻 | 2巻 |
| 開始ページ | 81 |
| 終了ページ | 88 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313719 |
| 著者 | 谷口 敏代| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11783 |
|---|---|
| タイトル（別表記） | The Neurophysiological Implications of an Atypical Slow Negative Potential in Short Interval CNV Paradigm |
| フルテキストURL | 001_091_097.pdf |
| 著者 | 岡本 基| 手蓑 正人| 中川 尚久| 森 宏和| |
| 抄録 | We recorded a slow negative potential from Cz (10/20 method) in 49 healthy students (12 male, 37 female, mean age 19.1) by a short interstumulus interval CNV paradigm. The interstimulus interval was 2 or 3 seconds, the warning stimulus presented at random or regular interval at 0.2 Hz. An atypical negative variation with two separated negative peaks was recorded in 26.0-30.6% trials regardless of interstimulus interval or modality of warning stimulus presentation, while a typical CNV was recorded in 32.0-59.2% of trials. No apparent negative variation was recorded in 14.3-18.4% in 2 seconds interstimulus interval, and 28-38% in 3 seconds interstimulus interval, showing that 2 seconds interval is better to get stable CNV recording than 3 seconds interval. The first negative wave of the atypical negative variation was 692-799msec in duration, but frequently prolonged to 1000msec or more in 3 seconds interval. It usually had negative peak around 900-1100msec, but sometimes around 1500msec. This features are different from any reported negative components of CNV. The second negative wave began 800-1200msec before second stimulus, and had its peak just before second stimulus, showing common features with readiness potential. The appearance of CNV was unsatble in the students in which the atypical negative variation was recorded in regular, 2 seconds intersitimulus interval, and the amplitude of slow vertex response and pattern reversal visual evoked potential was lower in thses students than in the students in which a typical CNV was recorded more than 3 times in total 4 times of trials. These findings indicate that the atypical variation observed in this study is due to a lowered arousal level or cortical neuronal activity, rather than a separated appearance of different components of CNV. |
| キーワード | contingent negative variation atypical slow negative potential arousal level slow vertex response visual evoked potential |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 91 |
| 終了ページ | 97 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313945 |
| JaLCDOI | 10.18926/11779 |
|---|---|
| タイトル（別表記） | An approach to English culture : in the case of Lawrence Country depicted in Lady Chatterley's Lover |
| フルテキストURL | 007_1_067_074.pdf |
| 著者 | 武田 光史| |
| 抄録 | 世界に先駆けて産業革命を成し遂げ七つの海を支配した英国の近代機械・産業文明の発展とは反比例するかのように破壊され消滅していく自然の森林と田園。それはかつて英国の中部一帯に広がっていたロビン・フッドの活躍したシャーウッドの森とて例外ではない。そこで現在わずかに残存しているその森の一部を含めてロレンス自身が「わが心のふるさと」と呼んだ生まれ故郷イーストウッドが、彼の最後の小説『チャタリー夫人の恋人』の中にどのように採り入れられ、どのように描写されているかを考察することにより、英国文化の偉大なる伝統が如何なるものであるかのその一端なりを論じてみる。 |
| キーワード | 英国文化 (English culture) 『チャタリー夫人の恋人』 (Lady Chatterley's Lover) D.H. ロレンスの故郷 (D.H. Lawrence Country) イーストウッド (Eastwood) 古き良き時代 (the good old days) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-09-30 |
| 巻 | 7巻 |
| 号 | 1号 |
| 開始ページ | 67 |
| 終了ページ | 74 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313931 |
| JaLCDOI | 10.18926/11778 |
|---|---|
| タイトル（別表記） | Determination of Hematinic Acid Produced by Oxidative Cleavage of Hemoglobin Heme in Red Blood Cells |
| フルテキストURL | 001_077_082.pdf |
| 著者 | 廣田 和弘| 佐々木 健二| 廣田 喬| |
| 抄録 | Our previous studies on the mechanism of phenylhydrazine-induced hemolytic anemia have shown that hematinic acid, one of oxidative cleavage products of heme, is formed by the reaction of hemoglobin with phenylhydrazine. Develoment of the determination of hematinic acid formed by this reaction in red blood cells (RBC) was required to study the mechanism of the hemolysis. Hemolysates prepared by lysis of fresh human RBC with water was mixed with standard hematinic acid. A solution consisting of hydrochloric acid, methanol, and acetone was added, and most of hemoglobin precipitated was removed by centrifugation. Hematinic acid was derived to the methyl ester by incubation with methanol containing sulfuric acid. The ester was passed to two type of silica gel column to remove interferences, and was analysed on a reversedphase high-performance liquid chromatographic column. Hematinic acid could be determined in the range 1.0-20.0μmol/ml RBC. Recovery from hemolysate was 65.0% ±3.5%. Standard compounds of hematinic acid and its methyl ester were prepared by the oxidation of hemin with hydrogen peroxide, and were comfirmed by elemental analyses and mass spectra. |
| キーワード | hematinic acid high-performance liquid chromatography red blood cells heme |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 77 |
| 終了ページ | 82 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313412 |
| JaLCDOI | 10.18926/11776 |
|---|---|
| タイトル（別表記） | Coping for the better quality of life(QOL) after adult kidney transplantation and factors affecting on the coping |
| フルテキストURL | 007_1_049_057.pdf |
| 著者 | 林 優子| |
| 抄録 | 腎移植は、免疫抑制剤の進歩に伴う高い生存率や生着率はもちろんのこと、今日では特に移植後のQOLに焦点が当てられている。移植後のQOLを向上させていくためには、レシピエントが移植後の様々な出来事にいかに対処していくかが重要である。本研究は、腎移植後のレシピエントの対処に焦点を当て、移植後のQOLに関係する対処および対処に影響を及ぼす要因を明らかにすることを目的とした。対象者は、関東の2施設で腎移植を受けて入院および外来通院をしている20才以上の10名のレシピエントで、研究参加の同意を得た後、腎移植後どのように対処しているかや、何が対処に影響を与えているかについて面接並びに観察による調査を行った。分析の結果、腎移植後の対処は＜問題と取り組む＞＜情報の交換＞＜問題状況の再認知＞である対処様式が主に用いられており、医師に任せるという＜お任せ＞は全員に見られなかった。また、腎移植後の対処に影響を及ぼす要因として、＜身体の状態＞＜自己概念＞＜不確かさ＞＜ソーシャルサポート＞が見いだされた。 |
| キーワード | 対処 (coping) 身体の状態 (physical state) 自己概念 (self concept) 不確かさ (uncertainty) ソーシャルサポート (social support) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-09-30 |
| 巻 | 7巻 |
| 号 | 1号 |
| 開始ページ | 49 |
| 終了ページ | 57 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313679 |
| JaLCDOI | 10.18926/11759 |
|---|---|
| タイトル（別表記） | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| フルテキストURL | 007_1_009_015.pdf |
| 著者 | 崎山 順子| 一村 光子| 唐下 博子| 遠藤 浩| 川上 香利| 河谷 友紀| |
| 抄録 | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| キーワード | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-09-30 |
| 巻 | 7巻 |
| 号 | 1号 |
| 開始ページ | 9 |
| 終了ページ | 15 |
| ISSN | 0917-4494 |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| タイトル（別表記） | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| フルテキストURL | 001_029_037.pdf |
| 著者 | 山本 剛禧| |
| 抄録 | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| キーワード | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 29 |
| 終了ページ | 37 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11756 |
|---|---|
| タイトル（別表記） | 大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 |
| フルテキストURL | 006_063_072.pdf |
| 著者 | 伊藤 昔子| 岡本 基| |
| 抄録 | Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA fragments inserted into the expression vector (pGEX-4T-1) were amplified by RT-PCR using mRNA purified from E18 rat brain as template. Primers attached with BamH I or EcoR I restriction site on 5' end were used to amplify first strand cDNA by PCR. Before ligation into the pGEX-4T-1 for GST fusion protein, PCR products were once cloned using T-A cloning system because they were not directly ligated into the pGEX-4T-1. E.coli strain BL21 was transformed by pGEX-4T-1 ligated with restriction DNA fragment cut out from pCR II plasmid vector of T-A clonig system. The growth of transformed BL21 was not different between the colony incubated at 37℃ for 24-48h and the colony stored at 4℃ for 7-10 days after 24h incubation at 37℃. The desirable OD(550) of culture medium for inducing the expression of fusion protein by isopropylthio-β-D-galactoside (IPTG) was from 0.6 to 1.0, because expression of native E.coli proteins per ml of culture medium was increased relatively when IPTG was added at OD(550) more than 1.0. The expression of fusion protein reached plateau around 6h after the induction. Relative expression of native E.coli proteins per ml of culture medium increased thereafter. Therefore, it may be desirable to purify the fusion protein around 6h after the induction. |
| キーワード | phosphacan (フォスファカン) glutathione S-transferase (グルタチオン-S-トランスフェラーゼ) BL21 IPTG fusion protein (融合蛋白) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-02-29 |
| 巻 | 6巻 |
| 開始ページ | 63 |
| 終了ページ | 72 |
| ISSN | 0917-4494 |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313693 |
| JaLCDOI | 10.18926/11751 |
|---|---|
| タイトル（別表記） | Nursing students' perception of their acquired basic skills in nursing practices : a comparison between a new and an old curriculum |
| フルテキストURL | 006_047_053.pdf |
| 著者 | 前田 真紀子| 近藤 益子| 太田 にわ| 池田 敏子| 中西 代志子| 高畑 晴美| 大井 伸子| 高田 節子| |
| 抄録 | 平成2年度改正の新カリキュラムで学んだ学生と旧カリキュラムで学んで学生間に、卒業時の基礎看護技術の到達度に違いがあるかを明らかにするために、看護技術の基本的要素、日常生活の援助技術、診療時の援助技術の49項目の到達度について、3年制課程の3年生に質問紙調査を行った。対象者全体では、到達度の上位10位以内に日常生活の援助技術項目の37％が含まれ、到達度の下位10位以内に診療時の援助技術項目の37％が含まれていた。新カリキュラム群と旧カリキュラム群の比較では、21項目に有意差があった。新カリキュラム群の高い15項目は日常生活の援助技術と看護技術の基本的要素に関するものが多く、旧カリキュラム群が高い6項目は診療時の援助技術が多かった。カリキュラム改正に関係なく、日常生活の援助技術の到達度は高く診療時の援助技術は低い傾向にあったが、カリキュラム改正後はその傾向が強まり、さらに看護技術の基本的要素の到達度はカリキュラム改正後に比較的高くなっていることが明らかになった。新カリキュラムでは臨地実習時間を削減しているが、基礎教育で重視する日常生活の援助技術と看護技術の基本的要素は習得できていると考えられる。 |
| キーワード | 基礎看護技術 (the basic skills in nursing practices) 到達度の学生評価 (students' perception of their acquirement) カリキュラム改正 (curriculum revision) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-02-29 |
| 巻 | 6巻 |
| 開始ページ | 47 |
| 終了ページ | 53 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313682 |
| JaLCDOI | 10.18926/11749 |
|---|---|
| タイトル（別表記） | メキシコ肺吸虫のセルカリアはどれか？ |
| フルテキストURL | 010_2_063_067.pdf |
| 著者 | 頓宮 廉正| |
| 抄録 | The true cercaria of Paragonimus mexicanus is the cercaria reported by Malek et al. in 1985 as a cercaria of P. peruvianus. However, there is a question whether the flame cell formula of 2〔(3+3+3+3+3)+(3+3+3+3+3)〕=60 is justified. Therefore, I question the possibility of the cercaria of P. mexicanus reported by Ito et al. in 1985. If it has a pseudo-sucker, it belongs to another species of Paragonimus cercaria. The cercaria reported by Tongu et al. in 1990 from Venezuela was eliminated the possibility of a new species. It is probable that this cercaria belongs to P. mexicanus. Ibanez revived the name of P. peruvianus in 1990 by the differences in the cercaria and adults. However, I disagree with his opinion. |
| キーワード | Paragonimus mexicanus (メキシコ肺吸虫) Paragonimus peruvianus (ペルー肺吸虫) cercaria (セルカリア) taxonomy (分類) Latin America (中南米) |
| 出版物タイトル | 岡山大学医学部保健学科紀要 |
| 発行日 | 2000-03-24 |
| 巻 | 10巻 |
| 号 | 2号 |
| 開始ページ | 63 |
| 終了ページ | 67 |
| ISSN | 1345-0948 |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313440 |