岡山実験動物研究会Acta Medica Okayama352019裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019組織・会則7879ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019会員名簿7373ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019会費に関する内規、会報投稿規程7272ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019第75 回、第76 回研究会例会、理事会報告5971ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019岡山実験動物研究会報第1 号から第３５号までの掲載内容4658ENKatsunoriSatoDepartment of Animal Resources, Tsushima South Laboratory, Advanced Science Research Center, Okayama University The Proceedings of the Okayama Association for Laboratory Animal Science were initially issued in 1983, the year following the establishment of the association, and No.35 is the latest.Scince issue No.2, the proceedings have consisted of the following content: <Greeting from the President>, <Summaries of Lectures (special, commemorative, and invited lectures), <Contribution>, <Introduction of Animal Research Facilities in Japan>, <Meeting Highlights>, <Member directory>, and <Organization and Rules of the Association>, in this order. Additionally, the publication of references and advertisements by supporting members recently started. Number 19 (issued in 2002) was the first to present a table of contents in English on its back cover, and this style has been maintained to date. On March 24, the president of the association and director of the Okayama University Libraries exchanged copies of an agreement regarding the registration of these contents with the Okayama University Scientific Achievement Repository (OUSAR) and other databases, providing a basis for their current registration and publication. Literature-based information is also being collected and published in databases organized by the Non-profit Organization Japan Medical Abstracts Society (Ichushi) and Japan Science & Technology Agency (JST).No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019地区研究会の活動紹介3845EN わが国には現在11 の地区研究会があり、独自の活動が展開されています。本報では、各地区研究会の間での情報交換、交流などを意図して、平成30 年度の活動について北から順に紹介します。なお、会長、事務局担当者及び講演者、話題提供者の方々のお名前は敬称を省略させていただきました。詳細な内容をご希望の方は各地区研究会の事務局にお問い合わせ下さい。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019実験動物アレルギー予防を目指した兵庫医科大学 病態モデル研究センターの紹介2937ENEijiSagaraCenter for Comparative Medicine, Hyogo College of Medicine Laboratory animal allergy (LAA) is an important problem on considering the occupational safety and health of animal workers. The Center for Comparative Medicine (CCOM), Hyogo College of Medicine, opened on April 1, 2018, is an animal experiment facility designed and constructed based on the concept of LAA prevention. This CCOM is located on the first and second floors of a newly built "education· research building". Methods for the prevention of LAA are airflow control by air conditioning equipment, breeding equipment, experimental equipment, cage transporters that suppress the diffusion of aero allergens, spraying of slightly acidic water mist, and prevention of allergen exposure by the strict use of Personal Protective Equipment (PPE). In addition, we implemented training on LAA and take measures to prevent office workers from being exposed to laboratory animal-derived allergens.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019コモンマーモセットを育てる2628ENHarunaHashimotoAdvanced Science Research Center, Okayama University In mid July 2018, the common marmoset bred in Okayama University gave birth to a triplet. It is very difficult to raise three infants. We selected the most heaviest one of her siblings, and we call her “POCKE”. We brought her up on the bottle for 80 days from the birth instead of her own mother. After 81 days, she finaly weand. Now she has become attached to human, but we hope that she gets back to their original community, as a “common” marmoset.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019新生児マウスを用いる動物実験が母マウスにもたらすストレスの生化学的評価2225ENNorioYataAdvanced Science Research Center, Okayama University Corticosterone, the glucocorticoid in rodents, is released from adrenal cortex in response to stress. Sometimes, for use in experiments, infants may be separated from their mother mouse. We studied the stress of mother mouse when she separates from infants. As a result, the concentration of corticosterone in the blood of the mother mouse tended to increase, by pulling her infants apart. In the case of using infant mice for animal experiments, it was suggested that it is necessary to consider the maternal stress.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019母マウスが望む環境エンリッチメントとは？ : 環境エンリッチメント選びを考える1821ENSumireIshiharaAdvanced Science Research Center, Okayama University Environmental enrichment, whose material is commercially available, is today thought to improve animal welfare, for instance, control of stress. There were some reports that these report contribution of suppression of stress, which the mother mice suffer during nursing their pup. However, the effects on stress-suppression by introducing the enrichment materials is unknown. In this study, determined plasma and urinary corticosterone in the mother mice with or without the materials from their making to weaning.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019種名が不明なミズハタネズミ亜科系統の種の同定1217ENSatoshiTakahataDepartment of Zoology, Faculty of Science, Okayama University of ScienceNatsumiIsemuraDepartment of Zoology, Faculty of Science, Okayama University of ScienceKazuyukiMekadaDepartment of Zoology, Faculty of Science, Okayama University of Science This study investigated the taxonomic status of a laboratory strain belonging to the family Arvicolinae for which the wild species name is unknown. This vole strain, though considered formerly a Lemming, was distinguishable from any Lemmus species by features of its appearance, skull and molar morphology, and conventional chromosomal pattern. Subsequently, we read the nucleotide sequence of the mitochondrial cytochrome b (Cyb) gene and constructed a molecular phylogenetic tree. We found that this vole strain belongs to the same clade as Microtus guentheNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019分泌型免疫グロブリンA の腸内細菌への結合活性は 高脂肪食の摂取により弱まる911ENTakeshiTsurutaGraduate school of Environmental and Life Sciences, Okayama UniversityTeresia AluochMUHOMAHGraduate school of Environmental and Life Sciences, Okayama UniversityKeiSonoyamaResearch Faculty of Agriculture, Hokkaido UniversityNaokiNishinoGraduate school of Environmental and Life Sciences, Okayama University Secretory immunoglobulin A (SIgA) is predominant antibody secreted into the gut relating to maintain gut homeostasis. In the present study, we focused on the SIgA coating of gut bacteria as a mucosal immune response affecting the gut bacteria following a high-fat diet (HFD). This study newly showed that the level of SIgA coating of gut bacteria was significantly decreased in HFD-fed mice than normal-fat diet (NFD)-fed mice. Furthermore, we found that the relative abundances of gut bacteria were significantly negatively or positively correlated with the level of SIgA coating of gut bacteria in NFD-fed and HFD-fed mice. Our observations suggest that a decrease in the level of SIgA coating of the gut bacteria through a HFD might relate to HFD-induced changes in bacterial composition.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019色素細胞系 : 生命機能発現機構解明の１モデルシステム28ENHiroakiYamamotoFaculty of Bioscience Nagahama Institute of Bio-Science and Technology There are two cell lineages of mammalian melanin pigment cells, one that leads to retinal pigment epithelium (RPE) cells in the eye from the optic cup and the other leading to melanocytes from vertebrate-specific neural crest cells. Melanocyte precursors have high mobility and migrate and settle into various tissues and organs, including the skin, choroid, inner ear, heart, brain, adipose tissue, lung, etc. It is well known that RPE cells are essential for visual acuity and that melanocytes in the skin provide protection against UV-damage. So, what is the function of extracutaneous melanocytes that are distributed elsewhere in the body where only very small amounts of light illuminate their existence? Here I would like to briefly introduce our research history and then discuss the important roles of melanocytes in the inner ear that are indispensable for hearing ability and those in the choroid where they seem to contribute to maintaining the structures of their habitats.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019会長あいさつ 11ENKatsumiMominokiDepartment of Animal Resources, Advanced Science Research Center, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama352019表紙・目次 ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018組織・会則9192ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018会員名簿8690ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018会費に関する内規、会報投稿規程8585ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018第73回、第74回研究会、理事会報告 7484EN寄稿（Contribution）は記入No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018岡山実験動物研究会の３５年間の活動5473ENKatsunoriSatoHonorary member in Okayama Association for Laboratoy Animal ScienceThe Okayama Association for Laboratory Science, established on December 7, 1982, celebrates its 35th anniversary on December 7, 2017. The association was established to provide opportunities for researchers interested in laboratory animals and animal experiments to assemble beyond the borders of universities, faculties, and institutions to exchange their knowledge and information, as well as help promote their research activities and advancement of community science. The executives, auditors, and other members of the association have organized and held workshops and issued newsletters under the leadership of former presidents. The association has been able to implement research activities for a long period of time thanks to the advice of regular members and the support of supporting members. The workshops have been held twice a year since 1990. A total of 74 workshops(regular conferences), as well as symposiums, have been held and 33 research bulletins (proceedings) have been issued over the last 35 years. We have summarized the workshops and symposiums etc. that have been held.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018地区研究会の活動紹介4553ENわが国には現在11 の地区研究会があり、独自の活動が展開されています。本報では、各地区研究会の間での情報交換、交流などを意図して、平成29 年度の活動について北から順に紹介します。なお、会長、事務局担当者及び講演者、話題提供者の方々のお名前は敬称を省略させていただきました。詳細な内容をご希望の方は各地区研究会の事務局にお問い合わせ下さい。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018鹿児島大学共同獣医学部総合動物実験施設の紹介4044ENAtsushiAsanoJoint Faculty of Veterinary Medicine, Kagoshima UniversityTo improve the quality of education and research with laboratory animals, Experimental Animal Center (EAC) was established as an animal facility in the Joint Faculty of Veterinary Medicine, Kagoshima University, on September 1st, 2015. We can house and use large-sized (bovine and horse), medium-sized (dog, cat, pig and bird), and small-sized (rodent and rabbit) animals in this facility. Our care and use program of laboratory animals in EAC was fully accredited by AAALAC International on June 9th, 2016. We are using this facility to demonstrate to our students that performance of veterinary care and animal welfare is important for appropriate education and scientific research with laboratory animals in Veterinary Medicine.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018弱酸性次亜塩素酸水溶液のアンモニアに対する消臭効果3639ENTomokoOnoResearch and Technology Development Division，HSP companyKojiYamashitaResearch and Technology Development Division，HSP companyThe deodorizing effect of weak acid hypochlorous solution against ammonia was investigated. When atomized particles of weak acid hypochlorous solution contacted ammonia, the ammonia concentration decreased in a concentration-dependent manner. The deodorizing effect was also observed against ammonia generated from the used floor cover for mice. When the floor mat was present, the ammonia concentration gently decreased but then rapidly decreased when the floor mat was removed. In the future, we will examine effective spraying conditions for weak acid hypochlorous solution in laboratory animal facilities, and we will also proceed with the validation of effects other than spraying.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018乳酸菌を貪食したマクロファージが放出する細胞外小胞の脂肪細胞 および骨格筋細胞への取り込み3235ENTakeshiTsurutaOkayama University Graduate School of Environmental and Life ScienceYoheiItoOkayama University Graduate School of Environmental and Life ScienceNaokiNishinoOkayama University Graduate School of Environmental and Life ScienceTeresia Aluoch MuhomahOkayama University Graduate School of Environmental and Life ScienceEmikoKatsumataOkayama University Graduate School of Environmental and Life ScienceKeiSonoyamaHokkaido University, Research Faculty of AgricultureNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018飼育下におけるハタネズミ2 系統のオスの性成熟について2831ENIbukiMatsumotoOkayama University of ScienceKeiryuTakeshitaOkayama University of ScienceKazuyukiMekadaOkayama University of ScienceIt is important to know the optimal timing of mating to produce laboratory animals efficiently and to ensure the preservation laboratory strains. To clarify the characteristics of the male sexual maturity of two laboratory Mictorus strains, namely the “Mar” and “MrosA” strains, which are derived from M. arvalis and M. levis, respectively, the testes and epididymes from 4-, 6-, 8- and 10 week-old males were weighed and compared. Although the tissue weights increased gradually with age in the Mar strain, in the MrosA strain, the weight of the testis increased markedly from 4 to weeks of age and that of the epididymis increased from 6 to 8 weeks of age. In addition, the spermatozoa concentrations in the cauda epididymis at 6, 8, and 10 weeks of age in were measured in each strain. The sperm concentration increased in the Mar strain at 8 weeks versus 6 weeks in the MrosA strain. From these results, it is clear that there are differences in the patterns of the increases in testis and epididymis weight and sperm concentration between the two strains. It was suggested that males of the MrosA strain attain sexual maturity earlier than those of the Mar strain.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018無腸動物Praesagittifera naikaiensis における細胞骨格要素2127ENRisaIkedaGraduate School of Education, Okayama UniversityChihoFujiwaraGraduate School of Education, Okayama UniversityMayukoHamadaUshimado Marine Institute, Faculty of Science, Okayama UniversityTatsuyaSakamotoUshimado Marine Institute, Faculty of Science, Okayama UniversityNoboruSaitoGraduate School of Environmental and Life Science, Okayama UniversityMotonoriAndoGraduate School of Education, Okayama UniversityAcoel flatworms can move in a variety of ways such as muscular and ciliary movements via cytoskeletal elements and their neural regulations. However, those locomotive mechanisms have not yet been fully elucidated. In this study, we examined the distribution of cytoskeletal elements including filamentous actin (F-actin) and tubulin, and the neuroanatomical organization in an acoelomorph worm, Praesagittifera naikaiensis (P. naikaiensis). Video microscopy revealed the elongation/contraction and the bending/rotation processes, and the ciliary gliding movement of P. naikaiensis. Histochemical and morphological analysis demonstrated that F-actin networks of inner longitudinal and outer circular muscle fibers were positioned along the entire surface of the body, and that the average distance between the circular muscle fibers in the contracted organism was decreased in the anterior region compared with that in the elongated organism. Electron microscopy showed dense bodies on the muscle cells of P. naikaiensis, which indicates that those muscle cells have the appearance of vertebrate smooth muscle cells. Immunohistochemical analysis revealed that -tubulin-positive signals on the ciliary microtubules had close contact with the F-actin network, and that neurite bundles labelled with anti dSap47 antibody as a neuronal marker run along the anterior-posterior body axis. These results indicate that the well-organized cytoskeletal elements and their neural control systems are preserved in P. naikaiensis, and that their mechanisms involved in those regulation systems are similar to those vertebrate systems. Further studies are needed to clarify the physiological mechanisms underlying the muscular and ciliary movements in P. naikaiensis.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018鶏コクシジウム症：アイメリア・テネラ感染メカニズムの解明に向けて1720ENToshimitsuHatabuGraduate school of Environmental and Life Sciences, Okayama UniversityAvian coccidiosis is most important entero-parasitic disease in the world. Eimeria parasite is causative agent of this disease. In Japan, this parasite species were widely spread and the positive rates are about 50 in layer and 70 % in broiler. The symptoms of coccidiosis are diarrhea, bloody excretion, weight loss, and die. Eimeria tenella is the most pathogenic protozoa. The sporozoites, infection form of this parasite, entry to epithelial cells around the crypt of cecum in early infection. After infection, parasites proliferate in epithelial cells, and form to sexual stage finally. However, we have less information about the pathophysiology, especially invasive mechanisms and infection route, by E. tenella infection. We have focused to analyze the invasive mechanism and route of this parasite because this phenomenon is first event to cause the pathophysiological changes in the infection. I would like to inform about Eimeria parasite and introduce our research in this paper.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018冬眠する哺乳類シリアンハムスターに学ぶ、冬眠可能な生体状態とは？1016ENYoshifumiYamaguchiDepartment of Genetics, Graduate School of Pharmaceutical Science, University of TokyoHibernation is an adaptive strategy for surviving during periods with little or no food availability, by profoundly reducing the metabolic rate and the core body temperature (Tb). Mammalian hibernators store fat extensively in white adipose tissues (WATs) during pre-hibernation period to prepare for hibernation. Thus, they undergo adaptive body remodelling in autumn, the pre-hibernation period, prior to hibernation, whereas little is known about the physiological and molecular mechanisms of the pre-hibernation remodelling. Syrian hamsters (Mesocricetus auratus) are facultative hibernators that can hibernate irrespective of seasons when exposed to prolonged short photoperiod and cold ambient temperature (SD-Cold) conditions. We found that before the initiation of hibernation, the body mass of Syrian hamsters decreased below a threshold, indicating that hibernation in this species depends on body condition. Global profiling of gene expression in various tissues of animals during the pre-hibernation period and hibernation period identified adaptive changes for hibernation at molecular level. Thus, Syrian hamsters as a model animal to study hibernation provide us a unique and convenient opportunity to analyse molecular and physiological mechanisms of adaptive pre-hibernation remodelling under a laboratory condition.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018生体内水銀の体外排泄に関する研究59ENHiromiAikohExtracorporeal excretion of mercury by two molybdenum complexes was investigated using mice after injection of metallic mercury or mercuric ion. After injection of metallic mercury into mice, the mice that received the incomplete cubane-type sulfur-bridged nitrilotriacetato molybdenum (IV) complex ([Mo3S4(Hnta)3]2-, referred to as the NTA complex) had the highest mercury content in their urine on the first day, followed by L-cysteine solution and then water. On the other hand, after injection of mercuric ion, the urinary mercury excretion from the mice that received various concentrations of the incomplete cubane-type sulfur-bridged cysteinato molybdenum (V) complex ([Mo2O2S2(cys)2]2-, referred to as the CYS complex) showed a higher level than that of mice that received water (control group). The mercury content in the heart and lungs of the group that received the CYS complex solution decreased in comparison with that of the group that received the water. At the same time, the mercury level in the kidneys for the CYS complex solution group showed the tendency to increase relative to the group that received water.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018マウス視床下部・下垂体・生殖腺系における転写因子Runx3の役割24ENSumioTakahashiGraduate School of Natural Science and Technology, Okayama UniversityRunx3 is a transcription factor that belongs to the Runx family. Female Runx3 knockout (Runx3−⁄−) mouse was anovulatory and infertile. Ovarian transplantation experiment suggested that lack of ovulation in Runx3−⁄− mice was caused by alteration of gonadotropin secretion in Runx3−⁄− mice. Cyp11a1 mRNA expression was less in Runx3−⁄− mouse ovaries than in wt ones. Hypothalamic Gnrh1 mRNA was increased, and Kiss1 mRNA expression in the anteroventral periventricular nucleus was decreased, but Kisspeptin mRNA in the arcuate nucleus was increased in Runx3-/- mice. Pituitary Fshb mRNA levels were increased in Runx3−⁄− mice. Cholesterol side-chain cleavage enzyme gene (Cyp11a1) expression was decreased in ovaries of Runx3−⁄− mice. These findings suggest that anovulation in Runx3−⁄− mice was partly due to the alterations in hypothalamus-pituitary-ovary system. Runx3 plays a key role in female reproduction through alteration of gonadotropin secretion.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018会長あいさつ 11ENTetsuoKuniedaGraduate School of Natural Science and Technology, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama342018表紙・目次 ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017組織・会則6566ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017会員名簿6064ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017会費に関する内規、会報投稿規程5959ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017第71回、第72回研究会、理事会報告4859ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017地区研究会の活動紹介4047ENOkayama Association for Laboratory Animal Science わが国には現在11 の地区研究会があり、独自の活動が展開されています。本報では、各地区研究会の間での情報交換、交流などを意図して、平成28 年度の活動について北から順に紹介します。
なお、会長、事務局担当者及び講演者、話題提供者の方々のお名前は敬称を省略させていただきました。詳細な内容をご希望の方は各地区研究会の事務局にお問い合わせ下さい。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017岐阜大学生命科学総合研究支援センター動物実験施設の紹介3539ENHidekiNikamiDivision of Animal Experiment, Life Science Research Center, Gifu University The facilities of laboratory animal within the Division of Animal Experiments, Life Science
Research Center, Gifu University was established in April 2005. In order to gain reliable data
from animal experiments, it is vital that the subjects' environment be stable and well-controlled. In addition, the safety impact to humans and bioethical rules must be considered when conducting animal experiments. We provide both the facilities necessary for such experiments as well as comprehensive support in the form of experiment program planning and subject care and maintenance.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017下垂体隆起部の発生期に特異的に発現する遺伝子Cytokine-like 1,Gap junction protein alpha 5の同定3134ENSayaka AizawaGraduate School of Natural Science and Technology, Okayama UniversityYurikoHigakiGraduate School of Natural Science and Technology, Okayama UniversityMahoOgoshiGraduate School of Natural Science and Technology, Okayama UniversitySakaeTakeuchiGraduate School of Natural Science and Technology, Okayama UniversitySumioTakahashiGraduate School of Natural Science and Technology, Okayama University Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars
intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well
studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during
the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017鳥類内耳tegmentum vasculosum の構造と機能2630ENRisaIkedaLaboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama UniversityTsuyoshiOtonoLaboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama UniversityNaoyaIkedaLaboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama UniversityNoboruSaitoLaboratory of Animal Physiology and Pharmacology, Department of Animal Science, Graduate School of Environmental and Life Science, Okayama UniversityMotonoriAndoLaboratory of Cell Physiology, Department of Science Education, Graduate School of Education, Okayama University In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017トゲマウスAco 系統の種の確定2225ENHitomiMochidaDepartment of Zoology, Faculty of Science, Okayama University of ScienceKazuyukiMekadaDepartment of Zoology, Faculty of Science, Okayama University of Science Spiny mice are useful in regenerative research and as a model for precocial animals. In the present study, we investigated the taxonomic status of the Aco strain, which is derived from spiny mice of an unidentified species. Although this Aco strain has been tentatively considered A. cahirinus species, it was distinguished from this species based on conventional and G-banding chromosomal patterns of this strain. Subsequently, we determined the nucleotide sequence of the mitochondorial gene cytochrome b (Cyb) and
constructed a molecular phylogenetic tree. We found that the Aco strain belongs to the same clade as A. dimidiatus. Based on this molecular analysis, we concluded that the Aco strain of spiny mice should be considered A. dimidiatus.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017カフェインによるアレルギー性鼻炎の抑制効果1821ENMadokaTsujimotoGraduate School of Human Life Sciences, Department of Foods and Human Nutrition, Notre Dame Seishin University AkikoSaikiGraduate School of Human Life Sciences, Department of Foods and Human Nutrition, Notre Dame Seishin UniversityManamiSuzukiGraduate School of Human Life Sciences, Department of Foods and Human Nutrition, Notre Dame Seishin UniversityToshiyukiShiragaGraduate School of Human Life Sciences, Department of Foods and Human Nutrition, Notre Dame Seishin UniversityYukioSugimotoOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesYasushiHayashiGraduate School of Human Life Sciences, Department of Foods and Human Nutrition, Notre Dame Seishin University We examined the effects of caffeine treatment on ovalbumin- and histamine-induced rhinitis mouse models and on corticosterone and catecholamine levels in mouse plasma. Treatment with caffeine decreased sneezing and nasal rubbing in both models, but direct nasal administration had no effect. Plasma levels of corticosterone and catecholamine, which have known anti-allergy effects, were increased by caffeine treatment. The increases in corticosterone and catecholamine may be associated with the suppression of rhinitis symptoms.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017遺伝子改変マウスを利用した生体内間葉系幹細胞の階層性の理解1517ENTakeshiTakaradaDepartment of Regenerative Science Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Determination of stem cell hierarchy/lineage is indispensable for a better understanding and
augmentation of many aspects of medical sciences, including the mechanisms of tissue development and maintenance of tissue homeostasis, as well as disease development. It also has implications in the field of tissue regeneration for medical treatments and disease modeling for drug discovery using iPS technology. Mesenchymal stem cells are multipotent stem cell that can differentiate into various type of cells including osteoblasts, adipocytes, myocytes and chondrocytes. Runt-related transcription factor 2 (Runx2) is an essential transcriptional regulator of osteoblast differentiation. Runx2 deficiency in Prx1+-derived cells
(Runx2prx1−/− mice) resulted in defective intramembranous ossification. Double-positive cells for Prx1-GFP and stem cell antigen-1 (Sca1) (Prx1+Sca1+ cells) in the calvaria expressed Runx2 at lower levels and were more homogeneous and primitive as compared with Prx1+Sca1− cells. Our results suggest that osteoblast differentiation in vivo may begin at the Prx1+Sca1+ MSC stage with sequential progression to Prx1+Sca1−cells, then Osx+Prx1−Sca1− osteoblast precursors, which eventually form mature α1(I)-collagen+
osteoblasts.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017腸内フローラ研究におけるノトバイオ―ト技術の貢献と進展1114ENHidetoshiMoritaGraduate School of Environmental and Life ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017ニワトリの食欲調節機構に関するこれまでの研究と今後の展望810ENKazuhisaHondaGraduate School of Agricultural Science, Kobe University Broiler chickens eat more feed than layer chickens. As a result, broiler chickens grow faster than layer chickens. However, excessive accumulation of body fat in broiler chickens has been a serious problem in the poultry industry in recent decades. Therefore, the appetite regulatory system of chickens has been a focus of research among poultry scientists. Lines of evidence suggest that the physiological role of peripheral adiposity hormones, such as leptin and insulin, and gut hormones, such as ghrelin, are different between mammals and chickens. Thus, species specificity make it difficult to understand the mechanisms underlying the regulation of food intake in chickens. Here I provide an overview of recent findings in this field and future perspectives.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017ラットを用いた薬物の経鼻吸収実験と吸収動態解析37ENDepartment of Pharmaceutical Science, School of Pharmacy, Shujitsu University Since a drug applied to the nose in an in vivo physiologic condition is translocated to the gastrointestinal (GI) tract by mucociliary clearance (MC), the drug undergoes absorption both from the nasal cavity and from the GI tract. The detailed MC of the rat was examined, using inulin as a marker of the applied solution. Inulin disappeared monoexponentially from the nasal cavity, indicating that the MC can be assumed to follow first-order kinetics. In the proposed kinetic model, the fractional absorption of the drug following nasal application is predicted as the sum of fractional absorption from the nasal cavity and fractional absorption from the GI tract, both of which are estimated indirectly from the permeability to the Caco-2 monolayer. This kinetic model is the first estimation system for nasal drug absorption based on drug disposition after nasal application and is useful for the development of nasal dosage forms.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017ミュータント系実験動物育種と野生動物の実験動物化22ENSen-ichiOdaFormer Department of Zoology, Faculty of Science, Okayama University of ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017会長あいさつ11ENTetsuoKuniedaGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017表紙・目次 ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama332017裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016組織・会則7677ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016会員名簿7175ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016会費に関する内規、会報投稿規程7070ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016第69回、第70回研究会、第4回実験動物科学シンポジウム、理事会報告5169ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016地区研究会の活動紹介4250ENわが国には現在11 の地区研究会があり、独自の活動が展開されています。本報では、各地区研究会の間での情報交換、交流などを意図して、平成27 年度の活動について北から順に紹介します。なお、会長、事務局担当者及び講演者、話題提供者の方々のお名前は敬称を省略させていただきました。詳細な内容をご希望の方は各地区研究会の事務局にお問い合わせ下さい。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016山口大学獣医学国際教育研究センター・先端実験動物学研究施設3741ENTohruKimuraAdvanced Research Center for Laboratory Animal Science (ARCLAS) within International Center of Veterinary Education and Research (iCOVER), Joint Faculty of Veterinary Medicine, Yamaguchi University was established in January 2015. The purpose of ARCLAS is twofold: first, to provide care and management for laboratory animals in safe and adequate manner and, second, to prepare the education curricula for practical training for laboratory animal science. I will now show the outline of newly developed laboratory facilities, ARCLAS.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016e-learning システムを用いた動物実験教育訓練知識確認テスト3336ENNorioYataChikaUefujiHarukoHirayamaKatsumiMominokiThe Animal Care and Use Committee, Okayama University have conducted the lecture, which is provided to researchers and students, who use the mammalian, avian and/or reptilian species for research. To use these species for research and education in our university, researchers and students must need to attend this lecture, pass the examination on the e-learning system, “the Okayama University WebClass”.This system is useful in order to improve the effectiveness of education and training.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016鳥類細胞における一過性遺伝子発現のためのプロモーターの比較2832ENToshiyukiKudoGenome editing technology by the CRISPR/Cas9, which was developed in 2012, is applicable in a variety of species. In birds, because the techniques of gene transfer and gene disruption has not been established, CRISPR/Cas9 method using adeno-associated virus (AAV) vector is the ideal combination as genome editing in vivo. However, in the use of AAV vectors, there is a problem to be solved that there is a limit on the size of the gene can be introduced. Therefore, it is important to minimize as much as possible the size of the gene. One strategy is to change the Cas9 gene into smaller ones, the other one is to minimize the promoter sequence. In this study, for the purpose of minimization of the promoter sequence to be introduced, the activities of the promoters to be general-purpose in mammals were verified in avian cells. CMV, CAG, and miCMV promoter shorten the CMV are all had sufficient activity in avian cells.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016C57BL/6 マウスの亜系統差について2227ENKazuyukiMekadaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016ウズラPBCF 遺伝子の構造と発現1921ENAyakoKugimotoSayakaAizawaMahoOgoshiSumioTakahashiSakaeTakeuchiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016岡山理科大学理学部動物学科における動物資源1218ENTakamichiJogaharaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016マウス卵子におけるミトコンドリアの特性911ENTakuyaWakaiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016岡山大学における動物実験に関わる機関管理体制の構築 ―非医療系キャンパスでの機関管理拠点として動物実験施設の整備コンセプトとその運用―58ENKatsumiMominokiIt has been important for academic research institutions to properly use the laboratory animals for research and education, pursuant to the provisions of the Law, Care and Management Standards, and the Fundamental Guidelines concerning animal experiments and related activities. However, in the several faculties, which do not have the centralized animal facilities, it appears that using the animals are inconsistent with these ordinances. To avoid that the inconsistency becomes any problems, Okayama University has been established new centralized animal facility to propel the institutional management. This content presents our concept of establishing the facility to properly conduct researchers and students in the above-mentioned faculties.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016「研究機関等における動物実験等の実施に関する基本指針」について24ENYasutakaKatsumataNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016会長あいさつ11ENTetsuoKuniedaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama322016表紙・目次ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015組織・会則9697ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015会員名簿9195ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015会費に関する内規、会報投稿規程9090ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015第67回、第68 回研究会、公開シンポジウム、理事会報告7489ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015実験動物科学シンポジウム7273ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015地区研究会の活動紹介6471ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015名古屋大学動物実験支援センター東山動物実験施設5963ENMarikoItohHideakiInagakiRaramiMoriMayumiTodaShuichiAonoJunSatoThe Institute of Laboratory Animal Research（ILAR）within the Center for Animal Research and Education (CARE), Nagoya University was established in July 2013. The aim of ILAR is to provide care for laboratory animals in safe and sanitary manner that enables researchers in the Nagoya University Higashiyama Campus to conduct proper experiments. We will now give an outline and
introduce the characteristics of the new ILAR.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015蝸牛管側壁におけるミオイノシトール輸送体タンパク質の発現5558ENMayumiYamajiRisaInoueMidoriEdamatsuMotonoriAndoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015視野の拡大を取り入れた「動物実験学」教育の試み5054ENKayoFurumotoToshinoriFurukawa入学時に動物実験に対し悪いイメージを持っている学生は少なくない。倉敷芸術科学大学生命科学部動物生命科学科では動物実験に対する正しい理解のため、医学およびバイオ分野における従来の動物実験の考え方のみならず、家庭動物を学びの対象とする動物看護分野における動物実験の考え方を取り入れることで、視点の拡大を目指す動物実験学を導入している。動物実験についての情報が少ない低学年では、「動物実験は残酷である」という部分に視点が集中し批判的な意見をもっている。しかしながら、学年が上がっていく過程で自身が動物実験を実施し、その必要性を実感する経験を繰り返すことで、動物実験を単に批判することはなくなっていく。4 年生では卒業研究における課題に科学的アプローチするために動物実験が必要な場合、多くの学生が動物実験の実施を前向きに選択するようにまでなる。学生の学びの対象に即した実験動物や動物実験の定義を教育に導入することで、動物実験への正しい理解へ繋がり、消極的容認が容認や肯定的態度へと変化していくと考えられる。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015実験動物を用いる実習における3Rs 実践の試み −マウス、ラットの飼育、保定、注射、解剖に向けての予備トレーニング−4049ENToshikiUsudaKayoFurumotoMegumuKita広島アニマルケア専門学校では、動物看護コース3 年課程（動物看護師志望者と実験動物技術者志望者が混
在）の3 年次に実施する実験動物学実習に際して、動物福祉の観点から動物に与える苦痛を可能な限り少なく
できる実施法を模索してきた。実際に生きた動物を用いる実習を行う前に、いくつかの予備トレーニングを取
り入れた3Rs 実践の試みである。具体的には、ケージ取り扱い練習、先鋭器具取り扱い練習のための雑巾縫
製、保定練習を目的とした軟質ボールによる手指トレーニング、自身の皮膚摘まみ練習、鉛筆や鶏手羽先を用
いた注射器具使用練習、そして餃子の皮、鶏皮、鶏手羽先を用いた解剖器具使用練習などである。これらの取
り組みそれぞれが、果たして効果的であるかどうか検証できているわけではないが、学生の取り組みを間近で
観察した立場から、感覚的には効果を実感している。また、予備トレーニングは、動物に与える苦痛を軽減す
ることを最大の目標とするものであるが、一方で、動物を用いた実習に臨む学生の心理的準備を促す効果もあ
るように感じている。実験動物技術者や動物看護師養成課程、コースが、大学や専門学校に設けられ、そこに
多くの学生が学んでいる現在、動物を用いた実習のプログラムを動物実験倫理・動物福祉の観点からさらに洗
練させることが望まれる。そのプログラムの一部として、われわれの予備トレーニングの取り組みを紹介した。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015マウス子宮内膜細胞におけるKallikrein の発現制御の解析3739ENMegumiTokumoriMahoOgoshiSakaeTakeuchiSumioTakahashiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015次亜塩素酸水溶液による環境消毒について3336ENKojiYamashitaYujiHamamotoYutoYasudaTomokoOnoWeak Acid Hypochlorous Solution (WAHS) has been used for disinfection of foods (meats, vegetables etc.), and for environmental disinfection in the Retirement homes, Hospitals and Laboratory Animal facilities. We will introduce here some of environmental disinfection tests. 1) To study whether WAHS is available or not for blood
blot inoculated by Acinetobacter baumannii on the plate comparing with Sodium Hypochlorite. 2) Comparison of Ethanol and WAHS on the floor and handrail. 3) To study efficacy of shallowly dipping by WAHS on wagon caster inoculated by Staphylococcus aureus.
The results are: 1) It was observed that WAHS had an efficacy equal to Hypochlorite with lower concentration in the blood test, but in case that the adhesion amount of blood was larger, much higher concentration or adding
physical removal was needed. 2) Ethanol and WAHS had Equivalent efficacy on the test of floor and handrail. 3) It was suggested that shallowly dipping by WAHS was available for disinfection of wagon caster. We hope to proceed to confirm how to use WAHS for environmental disinfection.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015ゼブラフィシュは知覚学習以前に反時計回りに左から右へ （L→R）泳ぎ、円形を避け倒立三角形に近づく1132ENKeiichiMittaniThe following results were obtained thorough induction and deduction by four experiments on
the early experience controlled zebrafish 4 males and 4 females using 30-30-40 milliseconds
behavior analysis system.
[1] It was found zebrafish perform two kind of movement. The first is high speed anticlockwise
(L→R) spiral circular swimming usig right hemisphere⇔left -visual field. The second is relatively
slowly clock (L←R) willing large elliptic swimming usig left hemisphere⇔right-visual field. If we
observe from the front of the tank, as DNA they almost always swim L→R basing probably on the
rotation and revolution of the earth (Experiment 1).
[2] In spite of the innate L→R swimming tendency, zebrafish prefer the visual black inverted
triangle side presented to the circle with the same peripheral length. They avoid circle and go to the
triangle in group following the leader without perceptual learning irrespective of the position
（Experiment 1,2,3,4）。
[3] In such a way, an inverted triangle and a circle are psychophysiologically quite different
geometrical configurations.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015Sfrp1 遺伝子は急性腎不全において腎線維化の進行を制御している810ENMakotoMatsuyamaRenal fibrosis is responsible for progressive renal diseases that cause chronic renal failure. Secreted Frizzled-related protein 1 (Sfrp1) is highly expressed in kidney, although little is known about connection between the protein and renal diseases. Here we focused on Sfrp1 to investigate its roles in renal fibrosis using a mouse model of unilateral ureteral obstruction (UUO). In wild-type mice, the expression of Sfrp1 protein was markedly increased after UUO. The kidneys from Sfrp1 knockout mice showed significant increase in expression of
myofibrobast markers, alpha-smooth muscle actin (SMA). Sfrp1 deficiency also increased protein levels of the fibroblast genes, Vimentin, and decreased those of the epithelial genes, E-cadherin, indicated that enhanced
epithelial-to-mesenchymal transition (EMT). There was no difference in the levels of canonical Wnt signaling; rather the levels of phosphorylated c-Jun and JNK were more increased in the Sfrp1-/- obstructed kidney. Moreover, the apoptotic cell population was significantly elevated in the obstructed kidneys from Sfrp1-/- mice following
UUO, but was slightly increased in those from wild-type mice. These results indicate that Sfrp1 is required for inhibition of renal damage through non-canonical Wnt/PCP pathway.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015鳥類の性決定とアロマターゼ遺伝子47ENToshiyukiKudoSex is the most important exchange method of the genetic information in eukaryotes. Sex determination mechanism is varied depending on the species, however, sex differentiation mechanism in vertebrates are basically similar. Sex determination of birds is controlled by the sex chromosomes and their sexual differentiation
are critically controlled by estrogen. Because estrogen is biosynthesized by aromatase, it is possible to find the sex-determining gene of birds by analyzing the transcriptional regulatory mechanism. While there have been conducting research on the basis of this concept, the mechanism has not yet been fully elucidated. On the other hand, DMRT1 gene has been cloned as a strong candidate in the sex-determining gene of birds. Analysis of cascade leading to the aromatase gene from DMRT1 gene is an important issue in the future.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015抗体の分子進化（親和性成熟）の研究： 面白い結果は思いがけなく訪れる23ENHitoshiOhmoriAntibodies are unique proteins in that they improve their affinity for antigens during
antibody responses. This process termed “affinity maturation” is the result of random
hypermutation of immunoglobulin genes in B cells followed by selection of the clones that acquired
high-affinity antibodies. However, what mechanisms are responsible for the induction of
hypermutation and the subsequent B cell selection in germinal centers has remained unknown in
many respects. I would like to talk about our long-lasting studies on these immunologic
mechanisms focusing on the functional role of follicular dendritic cellsNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015会長あいさつ11ENTetsuoKuniedaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama312015表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014組織・会則8485ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014会員名簿7983ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014会費に関する内規、会報投稿規程7878ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014第65 回、第66 回研究会、理事会報告6877ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014地区研究会の活動紹介6167ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014科学研究費補助金によるウズラの研究課題5060ENKatsunoriSatoJapanese quails are widely used for experiments are models of birds, such as chickens, and are becoming
increasingly important in research fields today. Their experimental usefulness was initially reported in an international
journal for academic research by Padgett et al. and Wilson et al. in 1959, and, since then, a large number of researchers
in- and outside Japan have conducted surveys and studies to examine their characteristics. In Japan, the first study on
Japanese quails with a Grant-in-Aid for Scientific Research was conducted in 1966, followed by a large number of
studies examining them. This grant program aims to support original and pioneering research activities, and is also
expected to promote studies on Japanese quails in a wide range of research fields, leading to the advancement of all areas.
This paper reviews previous studies on Japanese quails, conducted within the 48-year period between 1966 and 2013.
They were searched for using the Database of Grants-in-Aid for Scientific Research KAKEN, excluding some research
subjects, and are listed chronologically by keyword. Those comparing Japanese quails with other animals or using them
as part of the study are also included.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014新たに竣工した京都薬科大学バイオサイエンス研究センターの紹介4649ENTetsuNishikawaNew facility for laboratory animals; Bioscience Research Center (BSRC) was founded at Kyoto
Pharmaceutical University and it was due to work from September in 2013.
The BSRC aims the highest level of scale, equipment and contents within the pharmaceutical
universities in JAPAN and more proper animal experiments must be carried out.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014国立長寿医療研究センター研究所新実験動物施設棟の紹介4245ENNoboruOgisoSatomiTakanoKaoriMugurumaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014ニワトリにおける羽色調節の品種差3941ENToruTakahashiKaoriNishioMahoOgoshiSumioTakahashiSakaeTakeuchiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014マウス行動分析によるL-オルニチン長期投与の効果について3538ENSakiSogabeManamiSuzukiAkikoSaikiRikakoInoueYasushiHayashiL-ornithine has recently received considerable attention as an anxiolytic-like or antifatigue agent in
both human and animal studies. However, most previous results were obtained from a single
administration of L-ornithine. Accordingly, this study was designed to clarify the effects of long-term
L-ornithine treatment on psychological stress and locomotor activity in mice using a variety of behavioral
pharmacology methods. In a stress-induced hyperthermia test, 10 days of L-ornithine treatment induced
hyperthermia elevation. Pentobarbital-induced sleep time was shortened by the treatment. However, no
significant effects were detected in the elevated plus-maze, running wheel, or forced swim tests. These
results suggest that long-term treatment with L-ornithine elevates stress sensitivity and arousal level in
mice.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014わが国における統合医療は可能か2534ENYuzuruKurabayashiThe medical treatment called the alternative medicine or complementary and an Integrated medicine
is wide and it is said in recent years. It is assumed, "All of the medicine and the Medical treatment except
Western medicine that is taught in University Department of Medicine, and done in a general hospital"
with the alternative medicine. For instance, I want to examine whether integrated treatment that brings
these Alternative medicine and Western medicine treatment together can be done in our country in the
document about the strategy though there are Acupuncture & Moxibustion therapy, a Chinese medicine,
Supplement, Herb treatment, Ayulbadar, Yoga, Cairopractic, a Shiatsu and a Massage. Aromatherapy,
Homeopathy, Reflexology, a Tradition medicine, and a Folk remedy etc, and the Therapeutic gain is raised
by each method. It is necessary to build a Central hospital that integrated them according to various
places by the method of doing in China, to gather the expert in each treatment field, to make a best
treatment Method concept for the patient consulting, to do the system making to which it can treatment
equally immediately by all those who treat, and to practice the strategy immediately.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014創立３０周年を迎えた岡山実験動物研究会の追憶2124ENHiroshiNagaiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014実験動物福祉 −主観で語る覚悟を−1420ENMegumuKitaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014Hox 遺伝子と聴覚回路の発生1113ENYuichiNaritaKajari KarmakarSébastien DucretFilippo M. RijliSound vibration is sensed by hair cells in the inner ear. The information is transmitted to the cochlear nucleus in the
brainstem via spiral ganglion neurons. The information is further transmitted to higher relaying centers in the brain such
as superior olivary complex and inferior colliculus. The connectivity between these components is topographically
organized in a frequency-specific manner. It is known that the organization is well-established from the beginning of the
circuit development. However, little is still known about the molecular mechanisms underlying the development of
connectivity in the auditory circuit. Homeobox transcription factors of the Hox gene family are known for their
involvement in early anterior-posterior axis patterning of neuronal progenitors in the hindbrain. Recent evidence
indicates that they also play important roles in late aspects of neuronal development and establishment of topographic
circuitry. Moreover, a mutation in the HOXA2 gene has been recently shown to be responsible for hearing deficits in
humans. By means of spatiotemporally controlled Hoxa2 and Hoxb2 conditional mutations in the mouse we analyzed the
involvement of these factors in auditory circuit development and connectivity.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014実験動物を用いた酢酸の肥満抑制効果の評価810ENHiromiYamashitaAcetate is an endogenous metabolite of fatty acid β-oxidation in the liver mitochondria under
starved condition. Orally administered acetate is readily absorbed in the blood stream and then taken
up by tissues and activates AMP-activated protein kinase (AMPK) by increasing the AMP/ATP ratio.
Administered acetate shows a marked reduction in lipid accumulation in the adipose tissue, protection
against accumulation of fat in the liver, and improves glucose tolerance. It decreases the transcripts of
the lipogenic genes in the liver, indicating an inhibition of lipogenesis in that organ. Furthermore,
acetate treatment shows a higher rate of oxygen consumption and a smaller size of lipid droplets in white
and brown adipose tissues. It is indicated that acetate taken up has a potential to prevent obesity and
obesity-linked type 2 diabetes.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014筋ジストロフィーニワトリの水分代謝異常77ENNoboruSaitoThe muscular dystrophy chicken has been studying as model animal of muscular dystrophy for more
than 50 years. Recently, the mutation of WW domain containing E3 ubiquitin protein ligase 1 (WWP1)
gene has been identified as a responsible for muscular dystrophy chicken. We observed that muscular
dystrophy chicken not only showed the degeneration of skeletal muscles but also produced watery feces.
Therefore, we examined the possibility of abnormalities in water metabolism of muscular dystrophy
chicken. We first analyzed plasma osmolality and gene expression of aquaporin 2 (AQP2), AQP3 and
alpha subunit of the amiloride-sensitive epithelial sodium channel (αENaC) in muscular dystrophy
chicken and White Leghorn chicken under normal physiological conditions at five-week old.
Subsequently, we analyzed these same parameters after one-day water-deprivation. The main findings of
our study are that: I) the plasma osmolality was significantly higher in muscular dystrophic chicken than
in White Leghorn; II) kidney αENaC mRNA expression was significantly lower in muscular dystrophic
chicken than in White Leghorn; III) AQP2 and AQP3 mRNA expressions in muscular dystrophic chicken
were similar in White Leghorn. We suggest that the mutation of WWP1 may cause the abnormality of
sodium absorption, and thus muscular dystrophic chicken become hypernatremic.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014マウス初期胚発生におけるオートファジーの役割36ENSatoshiTsukamotoAfter fertilization, maternal products which are stored during oogenesis are rapidly degraded and newly zygotic
products are synthesized in most animals. This transition, known as an oocyte-to-embryo transition, is critical step for
further embryonic development. Considering that the process occurs quickly, bulk degradation system could be highly
activated to eliminate maternal products and recycle them to synthesize newly products. We have demonstrated that
autophagy, which is cytoplasmic bulk degradation system mediated by the lysosome, is highly induced after fertilization,
and autophagy-deficient embryo dies before implantation, suggesting that autophagy is essential for preimplantaion
embryonic development. Our recent studies also revealed that lysosomal size and number changes during the embryonic
development. Here we will briefly review the autophagy, and discuss the function of autophagy and lysosome in early
mouse embryogenesis.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama3020142014年度の会長あいさつ12ENSen-ichiOdaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama302014表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013組織・会則9899ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013会員名簿9397ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013会費に関する内規、会報投稿規程9292ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013第63回、第64回研究会、理事会報告8191ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013日本実験動物技術者協会第47回総会in晴れの国岡山8080ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013神戸大学医学研究科附属動物実験施設7679ENMasashiShiomiTakashiItoTomonariKoikeShun-ichiNakamuraNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013名水による生物生体に対するミコフ式・環境療法の研究(第4報)7075ENTakeshiIshiiToshihikoOkadaIsaoYamamuraAkinoriArimotoKoujiArimotoKyousukeWadaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013マウス子宮内膜におけるインスリン様成長因子結合タンパク質3の役割6669ENTetsuyaMaekawaSakaeTakeuchiSumioTakahashiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会30年のあゆみ6165ENKatsunoriSatoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013物質輸送における細胞関連携の重要性:心臓組織および内耳血管条組織を例として5560ENMotonoriAndoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013脊髄を介するグレリンの大腸運動促進作用5154ENHarukoHirayamaKatsumiMominokiTakahikoShiinaYasutakeShimizuNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013一級実験動物技術者特例認定制度の課題と展望4850ENKazuoOhwadaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013実験用ラット研究の進展4447ENTadaoSerikawaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013安全の科学とリスクコミュニケーション3743ENHideakiKarakiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会の30年間の活動1636ENKatsunoriSatoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会創立30周年を迎えて1515ENYuzuruKurabayashiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会創立30年を祝す1314ENMasashiKurimotoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会創立30周年を記念して612ENTakayoshiInoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会2013-2014年度会長の新任によせて45ENSen-ichiOdaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会の再出発(最終版:2012年度)23ENKeiichiMittaniNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013岡山実験動物研究会創立30周年記念写真ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama292013表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012組織・会則7879ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012会員名簿7377ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012会費に関する内規､会報投稿規程7272ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012第61回､第62回研究会､理事会報告6671ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012日本実験動物技術者協会第47回総会in 晴れの国岡山6565ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012倉敷芸術科学大学動物実験室および教育動物病院6364ENKayoFurumotoNoritakaMaedaTeppeiKandaSatoruKakeShouichiroYukawaNobuyoshiMuraoToshinoriFurukawaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012ICRマウスの遺伝子資源としての有用性5962ENYoshieYamamotoKozueUchio-YamadaKaoruTakanoAtsuoOguraToshihikoAsanoMasarouNakagawaICR(Yok:ICR)mice were introduced into the National Instltute of Infectious Diseases in 1965,and have been bred and produced there.Two inbred mutant mouse strains,MPS and ICGN,were created using(originated from)Yok:ICR mice in 1986.The MPS and ICGN mouse strains have served as the
animal disease models of mycoplasma infectious disease and congenital nephritic syndrome,respectively.
Genetic variations were detected by the genetic monitoring of Yok:ICR mice using biochemical markers.However, no difference was detected in gene expression profiles between the MPS and ICGN mouse strains.ICR mice may harbor genes that are spontaneously mutated,thereby allowing the creation of
new pathological models through the segregation and/or selection(screening)of mutant genes.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012アメリカにおけるOne Health Research の発展と展望(第3報)5858ENKaoriSasaokaJonnaA.K.MazetTakeshiIshiiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012温泉による生物生体に対する･ミコフ式･環境療法の研究(第三報)5057ENTakeshiIshiiHiroshiKimuraTosihikoOkadaReikoNobuharaIsaoYamamuraNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012生体の疼痛について4449ENYuzuruKurabayashiAbout the pain in the living body､In the feeling of the living body of the pain,the living body is proof to be alive.In causing the pain,Some abnormalities are often found in the internal organs that corresponds
to the part of the body.It is called internal organs parietal reflection in the Oriental medicine.The
function of internal organs can normalize in accentuation if some treatments are given from outside of the
body of the skin for this case.It is necessary to use and the analgesic regardless of strength..experiment..
Invading when the animal experiment treatment is given because there is a narcotic to which the action of relieving pain is weak,too,even if general anesthesia is given.The kind of the analgesic can
recommend non-steroid analgesic from an insignifficant thing of the certainty and the side effect in the
effect,and is technology in the animal experiment where the nociceptive stimulus is generated.There is a
layer structure of numerical layer in the corner part after hitting this entrance,and the kind of the
peripheral nerve fiber is also different though sense information from the tip enters the spinal cord
through the root after the spinal cord.It is necessary to consider enough analgesic use in the animal
experiment to which the generation of the pain is forecast.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012マイクロアレイによるニワトリ性的二色性の分子基盤の解析4043ENAyakaFukaoMinoriNakaokaSumioTakahashiSakaeTakeuchiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012弱酸性次亜塩素酸水溶液のスギ花粉アレルゲンCryj1に対する不活化効果3639ENTomokoOnoKojiYamashitaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012動物実験施設などに於ける脱臭･除菌を目的とした光触媒技術の応用3335ENShigeruTakaokaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012最近の実験動物用マイクロX線CT装置〜R_mCT2製品紹介〜2732ENIsaoHamanakaTakahisaSatoMinoruHosoi3DマイクロX線CT｢R_mCT2｣は､わずか17秒のX線掘影で512枚のCT画像を一気に得ることがで
きるCT装置である｡また､焦点サイズ5pmのX線管と高解像2次元検出器(FPD)を搭載し10pm画素の高
精細CT画像の撮影を可能としている｡本稿では､高精細な断層画像を高速撮影し3次元CT画像表示を可能
とするR_mCT2の特徴と､心拍同期撮影等の最新アプリケーションについて紹介する｡No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012実験動物と動物実験について今思うこと1526ENTetsuNishikawaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012植物培養細胞を活用して基礎研究と産学連携研究を追求して914ENHirokiHamadaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012日本産ナメクジウオの飼育コロニーの確立38ENKinyaYasuiDespite an intelest of long standing in the origin and evolution of vertebrates,recent contribution of studies on
lancelets(amphioxus),cephalochordates,to this field is not much.One of the major reasons for this is difficulties in
applying effective molecular techniques to this animal.To estabhsh a base of easy-use of lancelet materials,we are
improving a laboratorγ culture system of lancelets and have obtained sufficient spawns for six years.We also performed stocking with about 14,000 young lancelets at the native habitat in the Ariake Sea,Japan.With embryos obtained from the culture system,we first transformed epidermal cells of late neurulae with EGFP
expression vectors by electroporation method.Our culture System is is releaslng lancelet studies from constraint.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012岡山実験動物研究会の再出発（その5：2011年度）12ENKeiichiMittaniNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama282012表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011組織・会則7475ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011会員名簿6973ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011会費に関する内規､会報投稿規程6868ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011参考資料6367ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011第59回､第60回研究会､理事会報告5663ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011岡山大学自然生命科学研究支援センター動物資源部門津島北施設5255ENKatsumiMominokiRumiTakashimaAkihiroMatsukawaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011（独）放射線医学総合研究所実験動物開発・管理課の紹介4651ENTetsuNishikawaSince its founding in 1957, the National Institute of Radiological Sciences (NIRS) has been the only research and development institution in Japan dedicated to comprehensive research and development of radiation and health with various scientific disciplines. The main characteristics of laboratory animal experiments at NIRS are as follows: (1) the study of long-term biological effects on labol'8tory animals after exposure of various line types to nuclides of radioactive substances, (2) the use of animals maintained under strict quality control from a microbiological viewpoint, and (3) the use of wide range of imaging devices to capture images when
radioactive substances are used. We deal with various types of animals, from fish to primates, and the breeding and feeding periods of
these animals range from few days to approximately 2 years. Furthermore, special attention is given to carcasses and organs of laboratory animals that are treated as RI as well as nuclear fuel wastes, whereas such a restriction does not apply in regular experiments. There are 11 animal experimentation facilities at NIRS. The radiation control and animal management areas are located next to each other on the same site, and radiation workers at these areas are under supervision, strict management, and control. Safety education and training program are also provided to workers as a prerequisite. Not only from a Japanese but also from a global standard viewpoint, a high level of safety management is required for these facilities. During an external assessment in 2009, we received high evaluation marks from the Japanese Association of Laboratory Animal Facilities of National University Corporations and the Japanese Association of Laboratory Animal Facilities of Public and Private Universities for performing animal experiments with consideration of the ethical aspects and well-being of laboratory animals, as well as for the management of facilities.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011カリフォルニア大学群による第1回Global Health学会について（第2報）4545ENKaoriSasaokaJonna A.K.MazetPatriciaConradTakeshiIshiiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011電磁波による生物生体に対するミコフ式・環境療法の研究（第二報）4144ENTakeshiIshiiHiroshiKimuraToshihikoOkadaReikoNobuharaIsaoYamamuraNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011犬および猫の東洋医学的診断3640ENHiroshiTakeuchiSakuraImaiTomoyoKanayamaYuzuruKurabayashiIt is not easy to say that it is scientific clearly though in the Oriental medicine, there is a technique peculiar from the diagnosis to treatment. It is a development phase, and it is applied similar to the application to man though the Oriental medicine is being applied by the animal. This time, it explained the feature of an Oriental medicine disease in the dog and the cat. The method is the same as the method to do by the artery of the wrist that man has though Pulls with the groin artery is done in the dog and the cat. Moreover, the respiratory organ can be diagnosed,and because an inter-esting reaction to control it this time was admitted, it reports. On the other hand, it has a lot of volume of information though neither Eye and Pulls diagnosis are made a science. In addition, it reports on the diagnosis of the convulsion in the Oriental medicine this time. Moreover, this report is convinced of it that it is relate about the nervous system and pressure, and can do clarification scientific the Oriental medicine in the future.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011転写因子Pit-1の下垂体外組織における発現と下垂体ホルモンの発現制御3135ENShusukeTaniuchiSumioTakahashiSakaeTakeuchiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011雌マウス生殖器官におけるRunx3の役割2630ENYukikoTsuchiyaAtsukoSakumaSakaeTakeuchiSumioTakahashiRunx3(Runtdomaintranscriptionfactor3)はRunxファミリーに属する転写因子で雌マウスにおいてRunx3 mRNAは, 卵巣や子宮に発現していた。雌のRunx3(-/-)マウスは不妊であった。Runx3(-/-)マウスは卵胞形成異常を起こしており, 無排卵であった。一方で, 排卵能および黄体形成能は有していた。以上より, Runx3は卵胞形成および排卵制御に関与していることを明らかにした。Runx3(-/-)マウスの子宮は萎縮している。子宮内膜上皮細胞では, E2依存性の細胞増殖が起こらなかった。しかし,子宮内膜間質細胞では, E2, P4存在下で正常に細胞増殖が起きた。以上より, Runx3はE2による子宮の細胞増殖に関与していることを明らかにした｡No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011マイナスイオンの効果効能とオーリラの開発と試験について2225ENToruNagasawaNegative ion generators (O-RELA) produced by our company were developed for eight years jointly with Dr. Masahiko Maeda, a former researcher at the Yamagata Research Institute of Technology. The benefical effects of negative ions, and excellent characteristics of O-RELA are introduced in this paper.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011実験動物としてのウズラの有用性1621ENShinjiTakahashiAkiraShimizuTakaharuKawashimaThe Japanese quail has been familiar to our Japanese from ancient era (Heian, 1,000). In Edo era (1603-1868), male quails were housed in Samurai class as a pet of "Singing bird" to encourage his mind. After that, these birds were domesticated for poultry under the policy of Meiji government, JAPAN. However, the Japanese quail as an experimental animal was lately paid attention in the U.S.A after 1950. Since 1960 in Japan, the Japanese quail has been improved for an experimental animal by using the domesticated ones. The National Institute for Environmental Studies (NIES) has exploited the Japanese quail as a useful experimental animal for environmental science by cooperating with Tohoku University since 1980. By the way, the Japanese quail (Coturnix japonica) was selected for high (H) and low (L) antibody production to Newcastle disease virus (NDV) vaccine more than 50 generations. And H and L quails had been reached the plateau of NDV-HI (Haemo-agglutinin Inhibition) titer at 24(th) generation. The structure of major histocompatibility complex (MHC) genes of the H and L quails was examined and found that each line was independent MHC homozygous line. During the selection experiment, these H and L quails were examined in their utilization as an experimental animal for Environmental Science as follows: 1) The reproductive traits such as egg weight, egg production, hatchability declined in H and L line quails, and H line became extinct at 53 generation due to inbreeding depression. However, L line has been bred as inbred quail for avian experimental animal, because of hatchability recovery happened at 43 generation. 2) Among the 4 species (mice, rat, hamster, quail), the Japanese quail showed highest sensitivity to NO(2). On the contrary, the sensitivity to O(3) was lowest in quails. It has been suggested that toxic mechanisms of those gases are different from one another. 3) The environmental hormone (Endocrine disrupting chemicals, EDCs) was examined in the hybrid egg of quails in accordance with the OECD guideline. These results demonstrate that EDCs such as DDT, Dieldrin and TBTO will damage the reproductive organs and make the egg shells thin in adult female. In conclusion, these quails are useful not only as a pilot animal of poultry, but also as an experimental animal for environmental studies.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011両生類のように人間は再生できるようになるのか?1215ENAkiraSatohNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011川崎医科大学医用生物センターにおける管理運営の現状〜微生物統御､衛生管理を中心として〜811ENMarikoInoueTakanoriMikamiSatoruOgouTetsuyaOkinoSeitaroOhkumaThe Laboratory Animal Management Research Center in Kawelsaki Medical School is a felcility
maintaining laboratoly animals. It compnses five areas: 1) SPF (Specific Pathogen Free, newly established area), 2) Clean, 3) Conventional. 4) Infected animal. and 5) AdmInistratwn areas. Each area possesses a different microlnological standards grade. And the environment for the rearing of animals is kept clean and complies with the institutional standard concerning pathogens. We have managed this facility while especially taking care about the microorganism regulation and the sanitary management.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011マメジカの生理学的特性一原始的な反芻動物の形態・生理および習性ー37ENKatsuhiroFukutaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011岡山実験動物研究会の再出発（その4：2010年度）12ENKeiichiMittaniNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011倉林譲先生日本実験動物学会功労賞受賞ENNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama272011表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010組織・会則7374ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010会員名簿6872ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010会費に関する内規､会報投稿規程6767ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010参考資料6466ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010第57回､第58回研究会､理事会報告5864ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010岡山理科大学理学部動物学科棟（第28号館）5457ENNobuhikoAsadaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010小川勝士先生を偲んで5253ENYoshiroYabeNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010電磁波による生物生体に対するミコフ式・環境療法の研究（第一報）4751ENTakeshiIshiiHiroshiImuraTosihikoOkadaReikoobuharaIsaoYamamuraNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010カリフォルニア大学群におけるGlobal Healthおよびカリフォルニア大学､ ディビス校におけるOne Health Centerへの取り組みおよびカリフォルニアラッコの生態､歴史的背景､ 保護並びに研究4546ENKaoriSasaokaJonna A.K.MazetTakeshiIshiiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010ミヤマオウムNestor notabilisの性別判定4144ENKazuhiroYonedaDNAによる性判別法のミヤマオウムへの利用を目的として､鳥類の性染色体上に存在するCHD(chromo-helicase-DNA binding protein)遺伝子を標的とする2種類のプライマーセットについて検討した。その結果､ミヤマオウムでは､2種類のプライマーセットでDNAの増幅が認められた。しかし､オウム､インコ類においで性判別の報告があるプライマーAの雌雄間で認められる型が異なること
から判別ができなかった。一方､ワシ､タカ類のCHD-Wの配列を基に設計されたプライマーBを用いた方法では､ミヤマオウムのW染色体由来の増幅産物のみが検出されることから性判別が可能となった。また､対照として用いたヨウムは､2種類のプライマーセットで性判別が可能であったことから､プライマーBは､他の鳥種でも性判別のできる可能性が示唆された。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010ゼブラフィッシュは生得的に｢円｣パターンを避け「倒立三角形」パターンを視覚的に求める3740ENKeiichiMittaniExperiment 1 revealed that male four zebrafishs, aged 110 days after birth without pre-perceptual
learning of either black triangles nor black circles choose significantly more a inverted black triangle presented on the right saide to a black circlE' presented to the left side without food reinforcement. The outeriengths of both figures are same. Experiment 2 revealed that the same zebrafishs choose significantly more again the same inverted black triangle, presented on left side this time, to a black circle presented on right side.No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010揮発性有機化合物の胎仔期曝露による仔マウスの Thl､Th2型免疫応答のかく乱3336ENShojiYamamotoTin-TinWin-ShweHidekazuFujimaki胎児期､乳児期は化学物質に対して高感受性の時期であるとも云われており､多種多様な化学物質による子供の健康影響とそのメカニズムを把握することは生体機能の正常な発達の観点から重要と考えられている。本研究では低濃度トルエンの胎児期曝露が仔マウスの全身および局所でのTh1/Th2免疫応答に及ぼす影響についてグラム陽性菌細胞壁成分ペプチドグリカン(PGN; peptidoglycan)との併用, 非併用下で検討した。その結果､5,50ppm トルエンのみもしくはPGN刺激との併用曝露は血中や脾臓においてTh1及びTh2型免疫応答に関係するいくつかの免疫パラメーター(総Ig抗体サブクラスの産生レベルや転写因子T-bet, GATA-3, Foxp3のmRNA発現等)をかく乱することを明らかした。No potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010動物の経穴図はあるのか?2932ENYuzuruKurabayashiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010日本鶏における形態的・遺伝的多様性ならびにその利用1628ENMasaokiTsuzukiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010線虫C.エレガンスの遺伝子と遺伝子の虫1215ENHiroakiKagawaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010岡山大学農学部家畜育種学教室におけるマウスとウズラの研究紹介511ENKatsunoriSatoNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010花粉症と抗アレルギー薬の話34ENChiakiKameiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010岡山実験動物研究会の再出発（その3）12ENKeiichiMittaniNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama262010表紙・目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009裏表紙・英文目次ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009組織・会則8384ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009会員名簿7882ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009会費に関する内規､会報投稿規程7777ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009第55回､第56回研究会､理事会報告6976ENOkayama Association for Laboratory Animal ScienceNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009岡山大学自然生命科学研究支援センター動物資源部門鹿田施設6568ENKatsurniMominokiKazuhikoOchiaiNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009スンクスの消化管形態の巨視的観察と消化・吸収研究における スンクスの可能性5964ENToruTakahashiTomomiHaraMariNoborikawaYukaKawadaSen-ichiOdaNo potential conflict of interest relevant to this article was reported.岡山実験動物研究会Acta Medica Okayama252009遠赤外線による魚の成育に関する考察5058ENTakeshiIshiiHiroshiKimuraReikoNobuharaToshihikoOkadaHoshitoHukudaNo potential conflict of interest relevant to this article was reported.