検索結果 6178 件
| JaLCDOI | 10.18926/11921 |
|---|---|
| タイトル（別表記） | Comparative studies of proliferating cell nuclear antigen and bromodeoxyuridine staining pattern in rat regenerating liver cells |
| フルテキストURL | 005_079_085.pdf |
| 著者 | 﨑山 順子| 森 秀治| 一村 光子| 唐下 博子| 岡本 基| 遠藤 浩| |
| 抄録 | 種々の条件下（10％緩衝ホルマリン・6時間、1％パラホルムアルデヒド・6時間、100％メタノール・4時間、70％エタノール・6時間、100％アセトン・2時間、カルノア液・4時間）で固定したラット再生肝組織に免疫組織染色を施し、Proliferating cell nuclear antigen （PCNA）の核内染色パターンについての検討を行った。その結果、100％メタノール及び70％エタノールで固定した場合には、明瞭な顆粒状の染色像が得られた。10％緩衝ホルマリン及び1％パラホルムアルデヒドの場合には、明瞭な顆粒状並びに弱いびまん性の染色像が得られた。S期に対する代表的なマーカーとして知られるBromodeoxyuridine （BrdU）の染色像との比較のもと、再生肝組織中のPCNA陽性細胞の数とBrdU陽性細胞の数を再生ピーク時の術後1日目で検討したところ、100％メタノール及び70％エタノールで固定した際の陽性細胞数が、最もBrdUの所見と一致した。しかし、10％緩衝ホルマリン、1％パラホルムアルデヒド、100％アセトン、カルノア液で固定した場合には、BrdUの所見と著しい解離が見られた。これらの知見は、PCNAを指標に細胞増殖を評価する上で、100％メタノール、70％エタノールでの固定が有用であることを示唆する。 |
| キーワード | PCNA BrdU 免疫組織染色 (immunohistochemical staining) 再生肝 (regenerating liver) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1995-01-31 |
| 巻 | 5巻 |
| 開始ページ | 79 |
| 終了ページ | 85 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313417 |
| JaLCDOI | 10.18926/11875 |
|---|---|
| タイトル（別表記） | Ferrous Iron-induced Lipid Peroxidation in Cell Culture: Effects of Ferrous and Ferric Irons on Cell Survival and Serum Lipid Peroxide |
| フルテキストURL | 004_061_068.pdf |
| 著者 | 山本 剛禧| |
| 抄録 | Cell killing activities of ferrous and ferric irons (Fe(2+), Fe(3+)) in cultured 3T3 cells were investigated by the colony forming method in the culture medium containing 10% calf serum. In the colony forming method, small isolated colonies are seen in the dish which are the resulted of individual cells having undergone a series of cell divisions. Survival curve for Fe(2+)-induced loss of reproductive capacity (cell death) in 3T3 cells, showed a shoulder at low concentrations and only became exponential at high concentrations. Cells affected at 0.05mM Fe(2+) underwent apparently normal cell divisions, but at a much slower rate than the control. Survival rate at 0.5mM Fe(2+), which iron was added after seeding cells (added group), showed 0.75% but that at 0.5mM Fe(3+) did 19.3%. From the determination of Fe(2+) content carried out by the colorimetric method used N-PSAP, the oxidation of Fe(2+) was seen rapidly after adding Fe(2+) into the culture medium. In the cases of cells seeded 3 hours after adding Fe(2+) or Fe(3+) (preadded group), the survival rates showed much lower than those of the added groups, that is 55% at 0.5mM Fe(2+) and 89% at 0.5mM Fe(3+). The facts suggest that the radical reactions iniciated by the oxidation of Fe(2+) under the presence of cells, occured just after adding Fe(2+), play a crucial role in the damages on the adhesion of cells onto the dish and the following proliferation of cells. Serum lipid peroxides, as thiobarbituric acid reactive substances (TBA value), of the final cultured medium at colony counting time were measured. In the Fe(2+) added group, the TBA values more increased in proportion to the added concentrations of Fe(2+), whereas in the Fe(3+) added group, the increments of TBA values were very slightly and not to be depended on the concentrations. Furthermore, the TBA values of the Fe(2+) or Fe(3+) preadded groups were estimated as well as those of the added groups, respectively. The facts suggest that serum lipid peroxides induced by Fe(2+) have little effect on the proliferation capacity of cells in the level of cell culture. |
| キーワード | Cell Survival Ferrous and Ferric Irons Serum Lipid Peroxide |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1994-01-31 |
| 巻 | 4巻 |
| 開始ページ | 61 |
| 終了ページ | 68 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313910 |
| 著者 | ロドリゲス アンドレラ パオラ| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | プープイント ハン| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11843 |
|---|---|
| タイトル（別表記） | Relationship between intracellular uptake of adriamycin and membrane potential in ADR resistant Ehrlich ascites tumor cells |
| フルテキストURL | 003_081_085.pdf |
| 著者 | 浅海 淳一| 川崎 祥二| 西川 光治| 高献 書| 黒田 昌宏| 平木 祥夫| |
| 抄録 | We observed adiamycin (ADR) uptake and cellular transmembrane potential [amount of intracellular fluorescence of 3,3'- (Di-n-hexyl)- 2,2'- oxacarbocyanine iodide (NK-2280)] in ADR-resistant cells established from Ehrlich ascites tumor cells (EATC) and wild type EATC. In ADR-resistant cells, ADR uptake and the cellular transmembrane potential decreased as the degree of resistance increased. 4,4'- diisothiocyanatostilbene- 2,2'- disulfonic acid (DIDS) induced markedly decreases of ADR uptake and the cellular transmembrane potential. A good correlation was observed between ADR uptake and transmembrane potential in cultured cells. |
| キーワード | Adriamycin Cell Membrane Potential Flow Cytometry ADR-Resistant Cells DIDS |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 81 |
| 終了ページ | 85 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313508 |
| 著者 | 成島 道樹| |
|---|---|
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 三木 厚| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11830 |
|---|---|
| タイトル（別表記） | Ferrous Iron-induced Lipid Peroxidation in Cell Culture: preliminary experiments |
| フルテキストURL | 003_049_053.pdf |
| 著者 | 山本 剛禧| |
| 抄録 | To study the effect of Fe(2+) on the cell culture, preliminary experiments related to the lipid peroxidations of 3T3 cells and calf serum were carried out. Amonuts of lipid peroxides, as thiobarbituric acid (TBA)-reactive substances, were measured by means of the TBA color reaction under the acidic conditions by acetic acid, and were expressed as TBA value. The TBA value of calf serum in the phosphate buffered saline (PBS) with ethyrendiaminetetraacetate (EDTA) was lower than that of the control. The value in the presence of Fe(2+) showed significantly high level, but it was almost prevented by the addition of EDTA. Besides, Fe(2+)-induced lipid peroxides of serum in the PBS during the incubation at 37℃ were capable to measure by the application of EDTA. The TBA values of trichloroacetic acid (TCA) precipitates isolated from serum in the PBS and the culture medium were lower than that of control serum, but no difference was observed between the both TCA precipitates. The results suggested that the impurities in the culture medium were removed by TCA precipitation. Effects of Fe(2+) and EDTA on the TBA value of TCA precipitates were observed similarly to the case of control serum. Fe(2+)-induced lipid peroxidation of the cells in the PBS, which the peroxidation was stopped by the addition of EDTA, was observed, and it was accompanied with the lag time of induction period. The TBA value of the cells was more prevented by EDTA. The method in this paper may not be capable for the quantitative measurements of lipid peroxides in cells or serum, but is applicapable to measure the changes of relative amounts of lipid peroxides. |
| キーワード | Lipid Peroxidation Ferrous Iron Cell Culture |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 49 |
| 終了ページ | 53 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313457 |
| JaLCDOI | 10.18926/11822 |
|---|---|
| タイトル（別表記） | Sarcoidosis and Propionibacterium acnes |
| フルテキストURL | 003_019_036.pdf |
| 著者 | 中田 安成| 片岡 幹男| 木村 郁郎| |
| 抄録 | Although there have been numerous reports on the isolation of bacteria, fungus, and aetiological agents of chemical substances from specimens of patients with sarcoidosis, none of them have been substantiated. In any event, an understanding of the pathogenesis of pulmonary sarcoid is intimately linked to that of the processes involved in the accumulation of T-cells in the lower respiratory tract of individuals with sarcoidosis. Propionibacterium acnes was isolated at high rates and in high concetrations from lymph nodes in patients with sarcoidosis. However, the precise mechanism of granuloma formation and immunomodulation by P. acnes has not been elucidated yet. In patients with sarcoidosis, it was found that the high levels of interleukin-2 (IL-2) released from alveolar lymphocytes as well as interleukin-1, tumor necrosis factor (TNF) and interleukin-6 (IL-6) released from alveolar macrophages were stimulated by P. acnes. These cytokines (mainly IL-2), released by P. acnes in large quantities, play a major role in the compartmentalization of the T-cell population in the lung and lead to the formation of an alveolitis and granuloma in the lung parenchyma of patients with sarcoidosis. This paper focuses primarily on the role of the cytokine network in the pulmonary mononuclear cells in the lung of patients with sarcoidosis. |
| キーワード | sarcoidosis macrophage T-cell interleukin-2 Propionibacterium acnes |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 19 |
| 終了ページ | 36 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313339 |
| JaLCDOI | 10.18926/11820 |
|---|---|
| タイトル（別表記） | Photon background caused by the reduction of the electron beam energy - Materials of scattering foil - |
| フルテキストURL | 003_013_018.pdf |
| 著者 | 中桐 義忠| 三村 誠一| 稲村 圭司| 田原 誠司| 三宅 英昭| 東 義晴| 江草 具視| 三上 泰隆| 平木 祥夫| 山田 俊治| 杉田 勝彦| |
| 抄録 | The total skin electron beam therapy has been one of the clinical treatment for peripherally T-cell lymphoma; Mycosis fungoides, adult T-cell lymphoma, and so on. The crucial points in this treatment are not only having an optimum energy level of electron beam for a target volume (a tissue) but also keeping the photon back ground low. It is not easy to regulate those points by the control panel, however, for the equipment that is conventinally used for electron beam, theoretically, is to exchange lead (Pb), which is ordinarily used, to a low atomic number material as a scattering foil. We examined several different kinds and / or various thickness as a scattering foil material that can make the electron beam lower without an increase of the contaminant as X-ray. We hereby reported the results, and strongly suggested the following two materials in use; acrylic plate, carbon board, and so on, which are easily available and worked, would be practically useful for the total skin electron beam therapy. |
| キーワード | 電子線エネルギー X線混入率 電子線全身照射法 スキャタリングホイル |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1993-01-31 |
| 巻 | 3巻 |
| 開始ページ | 13 |
| 終了ページ | 18 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313375 |
| 著者 | 勝野 剛太郎| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 松村 拓大| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11778 |
|---|---|
| タイトル（別表記） | Determination of Hematinic Acid Produced by Oxidative Cleavage of Hemoglobin Heme in Red Blood Cells |
| フルテキストURL | 001_077_082.pdf |
| 著者 | 廣田 和弘| 佐々木 健二| 廣田 喬| |
| 抄録 | Our previous studies on the mechanism of phenylhydrazine-induced hemolytic anemia have shown that hematinic acid, one of oxidative cleavage products of heme, is formed by the reaction of hemoglobin with phenylhydrazine. Develoment of the determination of hematinic acid formed by this reaction in red blood cells (RBC) was required to study the mechanism of the hemolysis. Hemolysates prepared by lysis of fresh human RBC with water was mixed with standard hematinic acid. A solution consisting of hydrochloric acid, methanol, and acetone was added, and most of hemoglobin precipitated was removed by centrifugation. Hematinic acid was derived to the methyl ester by incubation with methanol containing sulfuric acid. The ester was passed to two type of silica gel column to remove interferences, and was analysed on a reversedphase high-performance liquid chromatographic column. Hematinic acid could be determined in the range 1.0-20.0μmol/ml RBC. Recovery from hemolysate was 65.0% ±3.5%. Standard compounds of hematinic acid and its methyl ester were prepared by the oxidation of hemin with hydrogen peroxide, and were comfirmed by elemental analyses and mass spectra. |
| キーワード | hematinic acid high-performance liquid chromatography red blood cells heme |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 77 |
| 終了ページ | 82 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313412 |
| 著者 | 趙 瑩| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 内田 亜希子| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11763 |
|---|---|
| タイトル（別表記） | Immunological Abnormalities of Alveolar Macrophages in Patients with Sarcoidosis |
| フルテキストURL | 001_039_050.pdf |
| 著者 | 中田 安成| |
| 抄録 | Bronchoalveolar lavage is an invaluable means of accurately evaluating the inflammatory and immune processes in the human lung. Sarcoidosis is a multisystem disorder characterized by heightened immune processes at sites of disease activity. The lung is most commonly involved. Although granulomas are charactaristic pathologic features of this disease, a number of studies suggest that the initial lesion in the lung is a T-cell alveolitis (an accumulation of T-cellls in the lung). There are a lot of findings that show abnormal functions of alveolar macrophages obtained by bronchoalveolar lavage in the release of various monokines and arachidonic acid metabolites and metabolize oxygen. In this review, the abnormalities of alveolar macrophages implicated in pulmonary T-cell alveolitis and fibrosis are reviewed and their potential roles in the lungs are discussed. |
| キーワード | sarcoidosis alveolar macrophage monokine metabolize oxygen arachidonic acid metabolite |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 39 |
| 終了ページ | 50 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313772 |
| 著者 | 原田 大輔| |
|---|---|
| 発行日 | 2007-03-23 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| JaLCDOI | 10.18926/11759 |
|---|---|
| タイトル（別表記） | 長期ホルマリン固定により失活したProliferating Cell Nuclear Antigen (PCNA) の免疫反応性回復条件の基礎的検討 ―マイクロウェーブ、オートクレーブの影響について― |
| フルテキストURL | 007_1_009_015.pdf |
| 著者 | 崎山 順子| 一村 光子| 唐下 博子| 遠藤 浩| 川上 香利| 河谷 友紀| |
| 抄録 | Using paraffin-embedded tissue sections of liver cancer obtained from autopsy which had been preserved in 10% buffered formalin solution for 6 months while PCNA immunoreactivity was lost, we examined the effects of heat processing by either microwave(MW) and autoclave(AC) in the presence of various processing solution. It appeared that AC processing took shorter time period than MW irradiation to restore equal immunoreactivity. With regard to immunoreactivity retrieval by MW irradiation,however, variation of the degree of retrieval depending on processing time was smaller than in AC, and so the stable consequences were obtained. Although AC processed tissues tended to be stained deep, prolonged processing time presented strong background staining and blurred nuclear margins which made it difficult to estimate the positive cell count. As for the effects of processing solution, there was little difference in retrieval of PCNA among 0.01 M citrate buffer (pH 6.0), saturated solution of lead thiocyanate and distilled water, but the least background staining was observed with distilled water. These observations suggest that MW irradiation of which effect of retrieval is less dependent of processing time and with the least background stainability, is superior to AC processing for PCNA immunoreactivity retrieval on formalin-fixed tissues. |
| キーワード | PCNA microwave (マイクロウェーブ) autoclave (オートクレーブ) immunohistological staining (免疫組織染色) formalin fixation (ホルマリン固定) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-09-30 |
| 巻 | 7巻 |
| 号 | 1号 |
| 開始ページ | 9 |
| 終了ページ | 15 |
| ISSN | 0917-4494 |
| 言語 | 英語 |
| 論文のバージョン | publisher |
| NAID | 120002313519 |
| JaLCDOI | 10.18926/11757 |
|---|---|
| タイトル（別表記） | Lipid Peroxides (TBA reactive substances)and Fatty Acid Compositions in Mouse Serum with Whole-body Irradiation and in Tumor-bearing Mouse Serum with Local Irradiation |
| フルテキストURL | 001_029_037.pdf |
| 著者 | 山本 剛禧| |
| 抄録 | Effects of irradiation on lipid peroxides of mouse serum, Ehrlich solid tumor-bearing mouse serum, which tomor cells were transplanted to the leg, and its tumor tissue were studied by a thiobarbituric acid (TBA) color reaction in the acetic acid condition with (Fe-TBA value) or without (TBA value) ferrous ammonium sulfate. TBA reactive substances were caluculated into the amount of malondialdehyde. Besides, fatty acid and lipid compositions were analyzed as a substrates. Irradiated samples were isolated at 3 days after irradiations. The TBA value of normal mouse serum was expressed 17nmoles/ml of serum, and Fe-TBA value gave 2.4-fold of the TBA value. Although an increase TBA value was not observed by whole-body irradiation, a significant increase of Fe-TBA value was measured, indicating 2.5-fold at 10Gy exposure compared with the normal Fe-TBA value, and 5.5-fold with the TBA value. The TBA value of tumor-bearing mouse serum was 14nmoles/ml as a low rate to that of normal serum, but the Fe-TBA value gave same magnification of that of normal one. The TBA value of tumor-bearing mouse serum was not changed by the local irradiation to the tumor region of leg, but the Fe-TBA value was increased 2.8 and 4.4 times at 10Gy and 20Gy exposures, respectively, comparing with the non-irradiated one, and 7.7 and 10.5 times with the TBA value, respectively. The TBA value of solid tumor homogenate exhibited 1.16nmoles/mg of protein, and the Fe-TBA value, pointed out 5 times as much as the TBA value. Both values slightly increased by the irradiation. These facts suggest that a shift of TBA value of serum hard to get, but Fe-TBA value of serum distinctly increases by the irradiation. The fatty acid composition of mouse serum lipids showed an increment of relative percentages of linoleic and arachidonic acids by the whole-body irradiation. The relative percentage of fatty acid compositon of lipids from solid tumor-bearing mouse serum was similar tendency to that of normal one, and the local exposure to the tumor part was not affected the each percentage. The percentage of high unsaturated fatty acid of tumor lipids similar to that of serum lipids, and a decrease of the percentage of arachidonic acid was accounted by the irradiation. In relative percentages of lipid compositions, the percentage of choleste rolester of serum lipids increased by the whole-body irradiation, and that of phospholipid fraction was not changed. In the case of tumor-bearing mouse serum lipids, an increment of the percentage of cholesterolester was obtained as compared with that of normal serum lipids, and this percentage decreased by the local irradiation to tumor part with an increment of the percentage of phospholipid. The percentage of phospholipid in tumor lipids had decreased by the irradiation. The facts suggest that a cause of the increased Fe-TBA value of tumor-bearing mouse serum by the irradiation to tumor is due to the release of peroxidizable phospholipid into serum from the damaged menbranes of tumor cells at large doses to exposure. |
| キーワード | Lipid peroxides Irradiation Mouse serum Tumor-bearing mouse serum Fatty acid composition |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1991-03-25 |
| 巻 | 1巻 |
| 開始ページ | 29 |
| 終了ページ | 37 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313903 |
| JaLCDOI | 10.18926/11753 |
|---|---|
| タイトル（別表記） | The effects of various fixating buffer soution in the electron microscopic observations |
| フルテキストURL | 006_055_061.pdf |
| 著者 | 赤塚 和也| |
| 抄録 | 生物学的あるいは生化学的な緩衝液を使用して作製された電子顕微鏡試料と従来の電子顕微鏡用として使用されている緩衝液で作成した試料とで電子顕微鏡像から比較検討した。HEPES緩衝液で作製した試料の電子顕微鏡像は、従来の電子顕微鏡に用いられている緩衝液で作製した試料から得られた電子顕微鏡像と非常によく似た像を示し、このHEPES緩衝液は電子顕微鏡の固定用緩衝液として使用できることが認められたが、その他（PIPES、MOPS緩衝液）の緩衝液ではあまりにも細胞内の可溶性物質が溶出しすぎて使用に耐えなかった。電子顕微鏡学研究において使用されうる固定用緩衝液は細胞内のオルガネラの保持が余りにも良い場合には、その微細構造の解析が困難になり、程々に細胞内の可溶性物質が溶出した方が解析がし易くなると思われ、この様な観点から、今回使用したHEPES緩衝液は生物学的あるいは生化学的な研究から引き続き電子顕微鏡用の試料を作製する際、同じ緩衝液が使用できることが認められた。 |
| キーワード | 電子顕微鏡 (electron microscopy) 微細構造 (ultrastructure) 肝細胞 (liver cell) カコジル酸緩衝液 (cacodylate buffer) HEPES緩衝液 (HEPES buffer) |
| 出版物タイトル | 岡山大学医療技術短期大学部紀要 |
| 発行日 | 1996-02-29 |
| 巻 | 6巻 |
| 開始ページ | 55 |
| 終了ページ | 61 |
| ISSN | 0917-4494 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| NAID | 120002313640 |