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    <Journal>
      <PublisherName>MDPI AG</PublisherName>
      <JournalTitle>Acta Medica Okayama</JournalTitle>
      <Issn>1999-4923</Issn>
      <Volume>18</Volume>
      <Issue>1</Issue>
      <PubDate PubStatus="ppublish">
        <Year>2026</Year>
        <Month/>
      </PubDate>
    </Journal>
    <ArticleTitle>Streamlined Radiosynthesis of [18F]Fluproxadine (AF78): An Unprotected Guanidine Precursor Enables Efficient One-Step, Automation-Ready Labeling for Clinical Use</ArticleTitle>
    <FirstPage LZero="delete">123</FirstPage>
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    <Language>EN</Language>
    <AuthorList>
      <Author>
        <FirstName EmptyYN="N">Xinyu</FirstName>
        <LastName>Chen</LastName>
        <Affiliation>Nuclear Medicine, Faculty of Medicine, University of Augsburg</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Kaito</FirstName>
        <LastName>Ohta</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Hiroyuki</FirstName>
        <LastName>Kimura</LastName>
        <Affiliation>Agency for Health, Safety and Environment, Kyoto University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Yusuke</FirstName>
        <LastName>Yagi</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Takanori</FirstName>
        <LastName>Sasaki</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Naoko</FirstName>
        <LastName>Nose</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Masaru</FirstName>
        <LastName>Akehi</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Tomohiko</FirstName>
        <LastName>Yamane</LastName>
        <Affiliation>Department of Molecular Imaging Research, Kobe City Medical Center General Hospital</Affiliation>
      </Author>
      <Author>
        <FirstName EmptyYN="N">Rudolf A.</FirstName>
        <LastName>Werner</LastName>
        <Affiliation>Department of Nuclear Medicine, LMU Hospital, and German Cancer Consortium (DKTK), Partner Site Munich, Ludwig-Maximilians-University of Munich</Affiliation>
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      <Author>
        <FirstName EmptyYN="N">Takahiro</FirstName>
        <LastName>Higuchi</LastName>
        <Affiliation>Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
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    <Abstract>Background/Objectives: [18F]Fluproxadine (formerly [18F]AF78) is a PET radiotracer targeting the norepinephrine transporter (NET) with potential applications in cardiac, neurological, and oncological imaging. Its guanidine moiety, while essential for NET binding, presents major radiosynthetic challenges due to high basicity and the harsh deprotection conditions required for protected precursors. Previous methods relied on multistep procedures, strong acids, and complex purification, limiting clinical translation. This study aimed to develop a practical one-step radiosynthesis suitable for routine and automated production. Methods: A direct SN2-type nucleophilic [18F]fluorination was performed using an unprotected guanidine precursor to eliminate deprotection steps. Reaction parameters, including the base system, solvent composition, precursor concentration, and temperature, were optimized under conventional and microwave heating. Radiochemical conversion (RCC) and operational robustness were evaluated, and purification strategies were assessed for automation compatibility. Results: Direct [18F]fluorination using the unprotected precursor reduced the total synthesis time to 60&#8211;70 min. Optimal conditions employed a tert-butanol/acetonitrile (4:1) solvent system with K2CO3/Kryptofix222, affording RCC up to 33% under conventional heating. Microwave irradiation further improved efficiency, achieving RCC of up to 64% within 1.5 min at 140 °C. The method showed broad tolerance to variations in the base molar ratio and precursor concentration and enabled isocratic HPLC purification. Conclusions: This one-step radiosynthesis overcomes longstanding challenges in [18F]fluproxadine production by eliminating harsh deprotection and enabling high-yield, automation-ready synthesis, thereby improving clinical feasibility.</Abstract>
    <CoiStatement>No potential conflict of interest relevant to this article was reported.</CoiStatement>
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        <Param Name="value">norepinephrine transporter</Param>
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        <Param Name="value">positron emission tomography</Param>
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        <Param Name="value">[18F]AF78</Param>
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        <Param Name="value">[18F]fluproxadine</Param>
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        <Param Name="value">radiolabeling</Param>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
      <Issn/>
      <Volume/>
      <Issue/>
      <PubDate PubStatus="ppublish">
        <Year>2025</Year>
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    <ArticleTitle>白居易の詩と女性――身近な女性を中心として</ArticleTitle>
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    <Language>EN</Language>
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      <Author>
        <FirstName EmptyYN="N">Hidenori</FirstName>
        <LastName>TACHIBANA</LastName>
        <Affiliation>Okayama University</Affiliation>
      </Author>
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    <CoiStatement>No potential conflict of interest relevant to this article was reported.</CoiStatement>
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        <Year>2025</Year>
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    <ArticleTitle>未利用資源の乳酸菌発酵による高付加価値化と化粧品原料への応用</ArticleTitle>
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    <Language>EN</Language>
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        <FirstName EmptyYN="N">Kanako</FirstName>
        <LastName>MATSUNAGA</LastName>
        <Affiliation>Okayama University</Affiliation>
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    <CoiStatement>No potential conflict of interest relevant to this article was reported.</CoiStatement>
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        <Year>2025</Year>
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    <Language>EN</Language>
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        <FirstName EmptyYN="N">Naochika</FirstName>
        <LastName>MATSUMARU</LastName>
        <Affiliation>Graduate School of Natural Science and Technology, Okayama university</Affiliation>
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        <Year>2025</Year>
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    <Language>EN</Language>
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        <FirstName EmptyYN="N"/>
        <LastName>TRAN NGUYEN THAO</LastName>
        <Affiliation>Graduate School of Natural Science and Technology, Okayama university</Affiliation>
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        <Year>2025</Year>
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    <ArticleTitle>Vesicular Glutamate Transporter 3 Is Involved in Glutamatergic Signalling in Podocytes</ArticleTitle>
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    <Language>EN</Language>
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        <FirstName EmptyYN="N">Naoko</FirstName>
        <LastName>NISHII</LastName>
        <Affiliation>Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University</Affiliation>
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        <Year>2025</Year>
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    <ArticleTitle>田上菊舎の詩諧研究</ArticleTitle>
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    <Language>EN</Language>
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        <FirstName EmptyYN="N">MENGHUAN</FirstName>
        <LastName>LI</LastName>
        <Affiliation>Graduate School of Humanities and Social Sciences, Okayama University</Affiliation>
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        <LastName>WEST STEPHEN BRANDON</LastName>
        <Affiliation>Graduate School of Humanities and Social Sciences, Okayama University</Affiliation>
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        <FirstName EmptyYN="N">YANG</FirstName>
        <LastName>LIU</LastName>
        <Affiliation>Graduate School of Humanities and Social Sciences, Okayama University</Affiliation>
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        <Affiliation>Graduate School of Humanities and Social Sciences, Okayama University</Affiliation>
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    <ArticleTitle>並列表現の史的展開と諸相−引用・疑問形式由来の「トカ」「トヤラ」を中心に−</ArticleTitle>
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        <FirstName EmptyYN="N">YUHENG</FirstName>
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        <Affiliation>Graduate School of Humanities and Social Sciences, Okayama University</Affiliation>
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    <ArticleTitle>Accuracy of deep learning-based attenuation correction in 99mTc-GSA SPECT/CT hepatic imaging</ArticleTitle>
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        <FirstName EmptyYN="N">Masahiro</FirstName>
        <LastName>MIYAI</LastName>
        <Affiliation>Graduate School of Health Sciences, Okayama University</Affiliation>
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        <Year>2025</Year>
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        <FirstName EmptyYN="N">Mengyao</FirstName>
        <LastName>XI</LastName>
        <Affiliation>Graduate School of Health Sciences, Okayama University</Affiliation>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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        <Year>2025</Year>
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        <FirstName EmptyYN="N">Takako</FirstName>
        <LastName>HIRAMATSU</LastName>
        <Affiliation>Graduate School of Health Sciences, Okayama University</Affiliation>
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        <Year>2025</Year>
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        <FirstName EmptyYN="N">Maki</FirstName>
        <LastName>DAITO</LastName>
        <Affiliation>Graduate School of Health Sciences, Okayama University</Affiliation>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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      <Volume/>
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        <Year>2025</Year>
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        <FirstName EmptyYN="N">ZIMO</FirstName>
        <LastName>LI</LastName>
        <Affiliation>Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University</Affiliation>
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  <Article>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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      <Volume/>
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      <PubDate PubStatus="ppublish">
        <Year>2025</Year>
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    <ArticleTitle>テラヘルツ波ケミカル顕微鏡による低分子生体関連物質の検出と高感度プロトコルの開発</ArticleTitle>
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      <Author>
        <FirstName EmptyYN="N">CHANGJIANG</FirstName>
        <LastName>LIU</LastName>
        <Affiliation>Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University</Affiliation>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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        <Year>2025</Year>
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    <ArticleTitle>大規模ネットワークにおける多重故障の高速迂回に関する研究</ArticleTitle>
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        <LastName>HERINIAINA Joseph Hermann Doris</LastName>
        <Affiliation>Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University</Affiliation>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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        <Year>2025</Year>
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    <ArticleTitle>Research on antiyeast effect of Lactiplantibacillus plantarum 3121M0s</ArticleTitle>
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        <LastName>Md. Bakhtiar Lijon</LastName>
        <Affiliation>Graduate School of Environmental and Life Science, Okayama University</Affiliation>
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      <JournalTitle>Acta Medica Okayama</JournalTitle>
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        <Year>2025</Year>
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        <LastName>LE VIET QUAN</LastName>
        <Affiliation>Graduate School of Environmental and Life Science, Okayama University</Affiliation>
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