ID | 30896 |
JaLCDOI | |
フルテキストURL | |
著者 |
Hayashi, Keiki
Okayama University
Hsueh, Chao-Liang
Okayama University
Kawasaki, Hideaki
Okayama University
Toyofuku, Hiroyuki
Okayama University
Miyashima, Takanao
Okayama University
Awai, Michiyasu
Okayama University
|
抄録 | Renal tissues from 208 human necropsies were observed histologically for disseminated intravascular coagulation (DIC). The tissues were stained with hematoxylin-eosin, Mallory's phosphotungstic acid hematoxylin (PTAH) and cationic ferric hydroxide colloid stabilized with cacodylate (Fe-Cac), and tested by immunoenzyme histochemical (IEH) reaction for fibrin-related materials (FRMs). The use of the IEH method increased FRM recognition, and FRMs were detected in a total of 80 cases (38.5%). In 26 cases diagnosed clinically as DIC, FRMs were shown in 23 of the cases (88.5%). Thus, 57 patients with FRMs were clinically asymptomatic. In rats with DIC induced by endotoxin injection, glomerulus FRM was effluxed into the tubulus through the Bowman's capsule and was excreted into urine. The electric charge was reduced on the endothelial surface of the glomerular capillaries in both human and rat DIC. Under the scanning electron microscopy, the endothelial surface appeared coarse in the glomerular capillary and fibrin degradation was present. Our conclusions are: (a) PTAH is non-specific for FRMs, (b) IEH aids the pathohistological diagnosis of DIC, especially in asymptomatic forms including the compensated DIC state, (c) FRMs in tubuli suggest DIC, and (d) DIC is possibly initiated by a reduction in the capillary electric surface charge. |
キーワード | disseminated intravascular coagulation
immunoenzyme-histochemistry
fibrinrelated materials
|
Amo Type | Article
|
出版物タイトル |
Acta Medica Okayama
|
発行日 | 1989-02
|
巻 | 43巻
|
号 | 1号
|
出版者 | Okayama University Medical School
|
開始ページ | 29
|
終了ページ | 38
|
ISSN | 0386-300X
|
NCID | AA00508441
|
資料タイプ |
学術雑誌論文
|
言語 |
英語
|
論文のバージョン | publisher
|
査読 |
有り
|
PubMed ID | |
Web of Science KeyUT |