検索結果 466 件
著者 | 岡村 容子| |
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発行日 | 1995-08-31 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 107巻 |
号 | 7-8号 |
資料タイプ | 学術雑誌論文 |
著者 | 遠藤 敦| |
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発行日 | 1995-08-31 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 107巻 |
号 | 7-8号 |
資料タイプ | 学術雑誌論文 |
著者 | 王 燕| |
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発行日 | 1998-06-25 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 110巻 |
号 | 1-6号 |
資料タイプ | 学術雑誌論文 |
著者 | 村上 宅郎| 蘇 衛東| 洪 珞珈| 朴 大勛| 大塚 愛二| 瀬尾 憲治| |
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発行日 | 1997-12-25 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 109巻 |
号 | 7-12号 |
資料タイプ | 学術雑誌論文 |
著者 | 掛橋 千賀子| 森 將晏| 川端 晃幸| 岡田 茂| |
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発行日 | 2001-04-28 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 113巻 |
号 | 1号 |
資料タイプ | 学術雑誌論文 |
著者 | 劉 慶平| |
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発行日 | 2002-05-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 114巻 |
号 | 1号 |
資料タイプ | 学術雑誌論文 |
著者 | 横田 修| 寺田 整司| 石津 秀樹| 黒田 重利| |
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発行日 | 2004-09-30 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 116巻 |
号 | 2号 |
資料タイプ | 学術雑誌論文 |
著者 | 大塚 愛二| |
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発行日 | 2005-05-20 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 117巻 |
号 | 1号 |
資料タイプ | 学術雑誌論文 |
著者 | 安田 華枝| 石原 武士| 寺田 整司| John Q. Trojanowski| Virginia M.-Y. Lee| 黒田 重利| |
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発行日 | 2007-01-04 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 118巻 |
号 | 3号 |
資料タイプ | 学術雑誌論文 |
著者 | 小野 廣| |
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発行日 | 2007-09-03 |
出版物タイトル | 岡山医学会雑誌 |
巻 | 119巻 |
号 | 2号 |
資料タイプ | 学術雑誌論文 |
著者 | MOHAMMED ABUL MANCHUR| |
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発行日 | 2008-03-25 |
出版物タイトル | |
資料タイプ | 学位論文 |
著者 | イスラム ブイヤン エムディ タリクル| |
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発行日 | 2007-03-23 |
出版物タイトル | |
資料タイプ | 学位論文 |
JaLCDOI | 10.18926/11925 |
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タイトル(別表記) | Effect of ferrous iron on 3T3-cell survival in cell culture |
フルテキストURL | 005_091_094.pdf |
著者 | 山本 剛禧| |
抄録 | 細胞培養レベルでの二価鉄の影響をマウス3T3細胞を用いてコロニー法でみた結果は、細胞播種直後の二価鉄添加(濃度0.5mMで0.75%の生存率)と培地に二価鉄添加後の細胞播種(濃度0.5mMで55%の生存率)で異なった細胞生存率が得られたことから、いずれも単細胞処理後の浮遊細胞に対する影響と考慮して、ディシュ面への接着後の培養細胞に対する二価鉄の影響を検討した。細胞播種1日後の二価鉄添加では濃度0.5mMで73%の生存率が得られ、上記条件下よりも高率の生存率が認められた。さらに生存の細胞コロニーの径は処理群で短縮がみられ、増殖阻害が明らかに認められた。細胞播種直後の二価鉄添加の生存率曲線から得られた損傷回復が影響されない最低濃度0.25mMでの二価鉄の時間的処理では、1時間処理では統計的に有意差は認められなかったが、3時間、6時間処理で有意差の増殖阻害が認められた。 |
キーワード | 二価鉄 (ferrous iron) 細胞生存率 (cell survival) |
出版物タイトル | 岡山大学医療技術短期大学部紀要 |
発行日 | 1995-01-31 |
巻 | 5巻 |
開始ページ | 91 |
終了ページ | 94 |
ISSN | 0917-4494 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313672 |
JaLCDOI | 10.18926/11875 |
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タイトル(別表記) | Ferrous Iron-induced Lipid Peroxidation in Cell Culture: Effects of Ferrous and Ferric Irons on Cell Survival and Serum Lipid Peroxide |
フルテキストURL | 004_061_068.pdf |
著者 | 山本 剛禧| |
抄録 | Cell killing activities of ferrous and ferric irons (Fe(2+), Fe(3+)) in cultured 3T3 cells were investigated by the colony forming method in the culture medium containing 10% calf serum. In the colony forming method, small isolated colonies are seen in the dish which are the resulted of individual cells having undergone a series of cell divisions. Survival curve for Fe(2+)-induced loss of reproductive capacity (cell death) in 3T3 cells, showed a shoulder at low concentrations and only became exponential at high concentrations. Cells affected at 0.05mM Fe(2+) underwent apparently normal cell divisions, but at a much slower rate than the control. Survival rate at 0.5mM Fe(2+), which iron was added after seeding cells (added group), showed 0.75% but that at 0.5mM Fe(3+) did 19.3%. From the determination of Fe(2+) content carried out by the colorimetric method used N-PSAP, the oxidation of Fe(2+) was seen rapidly after adding Fe(2+) into the culture medium. In the cases of cells seeded 3 hours after adding Fe(2+) or Fe(3+) (preadded group), the survival rates showed much lower than those of the added groups, that is 55% at 0.5mM Fe(2+) and 89% at 0.5mM Fe(3+). The facts suggest that the radical reactions iniciated by the oxidation of Fe(2+) under the presence of cells, occured just after adding Fe(2+), play a crucial role in the damages on the adhesion of cells onto the dish and the following proliferation of cells. Serum lipid peroxides, as thiobarbituric acid reactive substances (TBA value), of the final cultured medium at colony counting time were measured. In the Fe(2+) added group, the TBA values more increased in proportion to the added concentrations of Fe(2+), whereas in the Fe(3+) added group, the increments of TBA values were very slightly and not to be depended on the concentrations. Furthermore, the TBA values of the Fe(2+) or Fe(3+) preadded groups were estimated as well as those of the added groups, respectively. The facts suggest that serum lipid peroxides induced by Fe(2+) have little effect on the proliferation capacity of cells in the level of cell culture. |
キーワード | Cell Survival Ferrous and Ferric Irons Serum Lipid Peroxide |
出版物タイトル | 岡山大学医療技術短期大学部紀要 |
発行日 | 1994-01-31 |
巻 | 4巻 |
開始ページ | 61 |
終了ページ | 68 |
ISSN | 0917-4494 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313910 |
JaLCDOI | 10.18926/11847 |
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タイトル(別表記) | The results of liver function test on workers handling ethanol and isopropanol solvents |
フルテキストURL | 003_093_097.pdf |
著者 | 太田 武夫| 遠藤 浩| 森 秀治| 甲田 茂樹| 尾瀬 裕| |
抄録 | Liver function tests such as GOT, GPT, γ-GTP and ornithine carbamyl transferase (OCT) were done on workers handling alcoholic solvents. One was a group of molders working in a foundry where they sprayed ethanol solvent on the surface of a sandbox and burnt it. Another was a group of painters at an iron and steel works and they sprayed paint with isopropanol solvent on iron and steel plates. No statistical differences in the results of tests between the solvent workers and control groups were seen. High correlations were found between GOT/γ-GTP and OCT/γ-GTP. OCT of 4 workers who had drunk alcohol on the day before test showed a statisically high value compared to workers who had not drunk alcohol. |
キーワード | Ethanol Isopropanol Liver Function Test アルコール系溶剤 肝機能 |
出版物タイトル | 岡山大学医療技術短期大学部紀要 |
発行日 | 1993-01-31 |
巻 | 3巻 |
開始ページ | 93 |
終了ページ | 97 |
ISSN | 0917-4494 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313962 |
JaLCDOI | 10.18926/11830 |
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タイトル(別表記) | Ferrous Iron-induced Lipid Peroxidation in Cell Culture: preliminary experiments |
フルテキストURL | 003_049_053.pdf |
著者 | 山本 剛禧| |
抄録 | To study the effect of Fe(2+) on the cell culture, preliminary experiments related to the lipid peroxidations of 3T3 cells and calf serum were carried out. Amonuts of lipid peroxides, as thiobarbituric acid (TBA)-reactive substances, were measured by means of the TBA color reaction under the acidic conditions by acetic acid, and were expressed as TBA value. The TBA value of calf serum in the phosphate buffered saline (PBS) with ethyrendiaminetetraacetate (EDTA) was lower than that of the control. The value in the presence of Fe(2+) showed significantly high level, but it was almost prevented by the addition of EDTA. Besides, Fe(2+)-induced lipid peroxides of serum in the PBS during the incubation at 37℃ were capable to measure by the application of EDTA. The TBA values of trichloroacetic acid (TCA) precipitates isolated from serum in the PBS and the culture medium were lower than that of control serum, but no difference was observed between the both TCA precipitates. The results suggested that the impurities in the culture medium were removed by TCA precipitation. Effects of Fe(2+) and EDTA on the TBA value of TCA precipitates were observed similarly to the case of control serum. Fe(2+)-induced lipid peroxidation of the cells in the PBS, which the peroxidation was stopped by the addition of EDTA, was observed, and it was accompanied with the lag time of induction period. The TBA value of the cells was more prevented by EDTA. The method in this paper may not be capable for the quantitative measurements of lipid peroxides in cells or serum, but is applicapable to measure the changes of relative amounts of lipid peroxides. |
キーワード | Lipid Peroxidation Ferrous Iron Cell Culture |
出版物タイトル | 岡山大学医療技術短期大学部紀要 |
発行日 | 1993-01-31 |
巻 | 3巻 |
開始ページ | 49 |
終了ページ | 53 |
ISSN | 0917-4494 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313457 |
JaLCDOI | 10.18926/11801 |
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タイトル(別表記) | Measurement of Serum Lipid Peroxides used Thiobarbituric Acid Method: Colorimetric and Fluorometric Assays and Effect of Ferrous Ion |
フルテキストURL | 002_051_061.pdf |
著者 | 山本 剛禧| |
抄録 | Serum lipid peroxides were measured by a thiobarbituric acid (TBA) method. TBA color reactions carried out under the conditions with acetic acid (TBA-AA) or hydrochloric acid (TBA-HCl) systems, and then TBA value was detected by colorimetric ([OD]) and fluorometric ([FR]) measurements. Effects of ferrous ion, ferric ion and EDTA on the both systems were examined. Equal TBA value dependent upon concentrations of MDA (tetraethoxypropane) were obtained on both systems detected by [OD], but the relative fluorescence of TBA-AA system was higher than that of TBA-HCl system. Fluorometric determination was suitable for low TBA value, and colorimetric one was for high TBA value. Good correlation between TBA[OD] values and TBA[FR] values of serum from patients was estimated, but it was suggested that effects of serum substance were differ on the both systems. Effects of iron on TBA-AA and TBA-HCl systems were different significantly, particularly high Fe-TBA values obtained from TBA-AA system in the presence of ferrous ion. The fact suggests that serum lipid peroxidation is induced by ferrous ion during a heating of TBA color reaction. Same effect of ferric ion in TBA-AA system was observed. The increment of TBA values by ferrous and ferric ions was inhibited by a addition of EDTA. It was considered that the difference effect of iron on the both systems was due to pH and acetic acid concentration. The relationship of TBA values and Fe-TBA values of serum from patients was no correlated, and the various inhibition ratios of EDTA for the TBA value was calculated. It is suggested that Fe-TBA value is possible to detemine the qualitative difference of serum lipid peroxides. |
キーワード | Serum Lipid Peroxides Thiobarbituric Acid Ferrous Ion |
出版物タイトル | 岡山大学医療技術短期大学部紀要 |
発行日 | 1992-02-05 |
巻 | 2巻 |
開始ページ | 51 |
終了ページ | 61 |
ISSN | 0917-4494 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313802 |
JaLCDOI | 10.18926/fest/11513 |
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タイトル(別表記) | Decontamination of Waste-water by Metal Phthalocyanines Encapsulated in Polymeric Microcapsules |
フルテキストURL | 008_097_101.pdf |
著者 | 高見 浩二| 吉澤 秀和| 阪田 功| 北村 吉朗| |
抄録 | Polymeric microcapsules encapsulating iron phthalocyanine (Fe-Pc) and zinc phthalocyanine (Zn-Pc) were successfully prepared in this study. Absorption spectra of polymeric microcapsules encapsulating metal phthalocyanines were measured in visible light range. It was expected from the results that the polymeric microcapsules encapsulating metal phthalocyanines showed high photocatalytic activity upon irradiation with visible light such as sunlight. The catalytic and phtocatalytic activities of encapsulated Fe-Pc and Zn-Pc were investigated in the decomposition of methylene blue in water. The encapsulatedd Fe-Pc with H(2)O(2) exhibited a high catalytic activity. Methlene blue was efficiently decomposed by interaction of Fe-Pc with H(2)O(2). |
キーワード | microcapsule decomposition metal phthalocyanine waste-water treatment suspension polymerizatio |
出版物タイトル | 岡山大学環境理工学部研究報告 |
発行日 | 2003-03 |
巻 | 8巻 |
号 | 1号 |
開始ページ | 97 |
終了ページ | 101 |
ISSN | 1341-9099 |
言語 | 日本語 |
論文のバージョン | publisher |
NAID | 120002313830 |
タイトル(別表記) | Effects of Mannosen on Hydroponically Grown Barley |
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フルテキストURL | 003_002_129_135.pdf |
著者 | 柴坂 三根夫| 宮田 將彦| 秋山 佳子| 河﨑 利夫| |
抄録 | To examin the effects of mannose on iron absorption of barley roots,barley was hydroponically grown for 36 days in a greenhouse. Potassium and phosphate of barley plants grown in a diluted mannose solution were obserbed at similar as the controls. On the otherhand, some morphological changes were observed in mannose-treated barley plants. |
キーワード | Barley Hydroponics Mannose Potassium Phosphate |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 129 |
終了ページ | 135 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |
タイトル(別表記) | Differences in the Responses to Iron Deficiency Stress between Bean and Maize |
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フルテキストURL | 003_002_117_127.pdf |
著者 | 米谷 力| 森次 益三| 河﨑 利夫| |
抄録 | The responses to iron deficiency stress in bean and maize were compared. The susceptibility to iron deficiency stress was smaller in bean than in maize;i.e., the tolerance to iron deficiency was greater in bean than in maize. The roots of the bean plants exposed to iron deficiency stress, developed iron reducing capacity and medium-pH lowering capacity,but not the roots of maize. The iron reducing capacity and medium-pH lowering capacity of the bean roots were inhibited by a shadowing, detopping, and the addition of 2,4-dinitrophenol. Iron absorption in the bean plants was stimulated by the pretreatment without iron in the growth medium, but not in the maize plants. The finding suggest that the high tolerance of bean plants to iron deficiency stress is caused by the development of iron reducing capacity and medium-pH lowering capacity of the bean roots. |
キーワード | Bean Iron deficiency stress Iron reducing capacity Maize Medium-pH lowering capacity |
出版物タイトル | 岡山大学資源生物科学研究所報告 |
発行日 | 1995 |
巻 | 3巻 |
号 | 2号 |
開始ページ | 117 |
終了ページ | 127 |
ISSN | 0916-930X |
言語 | 日本語 |
論文のバージョン | publisher |