ヒト正常肝のレチクリン線維とグリソン氏鞘域間質物質の分離とその構成成分の検討 ―特にcollagen組成の差異について―

Intralobular (A) and interlobular (B) tissue fragments of the liver were separated from normal human liver tissue by tissue fractionation with a metal mesh. Connective tissue extracted from A was composed of reticulum fibers and that from B was thick and contained vascular structures, and a small amount of muscle and elastic fibers. It was revealed by histological methods that the tissue fragments and connective tissues retained the integrity of intra- and interlobular tissue as observed in vivo. These connective tissues were solubilized with either pepsin in acetic acid or 4 M guanidine-HCl, and analyzed for the amount of collagen, neutral sugar, fibronectin, laminin and hexuronic acid. Pepsin digestion resulted in solubilization of about 70% of total collagen. Collagen type analysis revealed that 29% was type I, 28% was type III and 43% was the other types in intralobular connective tissue, and that 42% was type I, 29% was type III and 29% was the other types in interlobular connective tissue. Guanidine-HCl extraction resulted in solubilization of 32.4% (A) and 22.1% (B) of total neutral sugar. The concentration of fibronectin and laminin in the guanidine extract were 16.4 μg and 53.6 ng per 1 g of A, and 3.4 μg and 157.0 ng per 1 g of B. Guanidine-HCl extraction resulted in solubilization of 41.8% (A) and 24.2% (B) of total hexuronic acid, which accounted for 3.1 mg/g and 1.3 mg/g, respectively. These results indicated that the two connective tissues histologically defined as intralobular and interlobular connective tissue were different in the amount of components.