胆汁成分と肝臓組織 第2編 胆汁に作用させた肝組織中の乳酸脱水素酵素の消長

I have observed the prosperity and decay of the dehydrating enzyme of lactic acid in the liver tissues of the healthy guinea pigs, the guinea pigs with the use of carbon tetrachloride and the guinea pigs with the obstruction of the retieuloendothelial system without any preparation and with the effection of the rabbits' bile. The results are as follws: 1) The liver tissue of the healthy guinea pig works to fade methylene blue and the process of the discoloration is more promoted by the addition of Sodium lactic acid. 2) Even though it's action become markedly decreased, the surface of the liver tissue of healthy guinea pig effected with the bile has the reducting action of methylene blue and it's action become promoted by the addition of sodium lactic acid, but the reducting action become low by the process of the times which the liver tissue was effected with the bile. At that time, the decline of the methylene blue reducting action at the surface of the liver tissue, it comes up to the 3 or 5 layers of the cells in depth. 3) The same condition is observed on the liver tissue of the guinea pig with the use of carbon tetrachloride and the obstruction of the reticuloendothelial system, but comparing with the healthy case, the reducting action of methylene blue become markedly weak. And comparing between the above two cases, the reducting action of the later is more weak than the former. Therefore, the effect to the reducting action of methylene blue by the effection of the bile is not shown clearly. 4) The report of Dr. Horio in our department that the activity of the dehydrating enzyme of lactic acid become decreased in the cases with the function impediment of the reticuloendothelial system, is adequate. 5) In the cases of the liver tissues effected with the bile, the decreased process of the dehydrating enzyme of lactic acid at the surface of the cutting tissue of the liver is in parallel with the reduction's process of biliverpin to bilirubin in the bile and the tissue and the dehydrating enzyme of lactic acid working on it is only the enzyme on the surface of the cutting tissue of the liver.