人癌及びマウス癌組織の組織培養に関する研究 第3編 人子宮頸癌及びBashford氏マウス癌並びに正常マウス肝組織の組織培養に於ける蛋白代謝に就いて

The protein metabolism of human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue have been studied with special reference to the culture media, and the following results were obtained. 1. The whole protein in the culture media of human cervical carcinoma, Bashford's mouse carcinoma, and normal mouse liver tissue was estimated. And as the results it was found that with lapse of time of the culture, human cervical carcinoma and Bashford's mouse carcinoma showed a higher protein consumption than that by normal mouse liver tissue. 2. In every tissue culture albumin was increased as compared with that in the control, but there was no difference between tumor tissues and normal tissue. 3. α-globulin in the media of human cervical carcinoma had no deviation from the control. α-globulin in the media of Bashford's mouse carcinoma showed a derease when compared with that of the control on one day after tissue cnlture, but with lapse of time in tissue culture it was increased. In the culture media of normal mouse liver tissue, α-globulin showed an increase as compared with the control on one day after tissue culture, but a remarkable decrease was found on 3 days afterward. As for normal mouse liver tissue, α-globulin showed the lower value than human cervical carcinoma and Bashford's mouse carcinoma during the course of tissue cultures. 4. The remarkable increase of β-globulin in the culture media of human cervical carcinoma could be seen. In the media of Bashford's mouse carcinoma, β-globulin showed a decrease on 3 days after tissue culture. β-globulin in the culture media of normal mouse liver tissue revealed an increase on one day after tissue culture, but on third day Bashford's mouse carcinoma and normal mouse liver tissue presented almost the same value. And both tissues showed an increase on five days cfter the tissue culture. 5. γ-globulin in every tissue culture was found to show a decrease as compared with the control along with the lapse of culture time. And in this respect no difference was found between tumor tissues and normal tissue.