Effects of caffeine on the longevity and locomotion activity of the common green bottle fly, Lucilia sericata (Diptera: Calliphoridae)

The common green bottle fly, Lucilia sericata (Meigen) (Diptera: Calliphoridae), is a promising and useful managed pollinator for greenhouse agricultural crops. The fly can pollinate at lower and higher temperatures than European honeybee. However, management of the longevity of pollinators is important for growers using greenhouses. Previous studies using other insects showed that caffeine affects insect longevity and behaviors. For instance, European honeybee live longer and have increased memory after caffeine consumption. How caffeine affects the longevity and behavior of pollinators is worth investigating because it can affect pollinator’s behavior, extend longevity, or be an insecticide against pollinators. In the present study, therefore, the longevity and locomotion of L. sericata were investigated when they were given different caffeine concentrations. First, the longevity of L. sericata with five different caffeine concentrations was compared to the control. The results showed that higher concentrations of caffeine (2%, 1%, and 0.5%) significantly decreased the life span compared to lower concentrations (0.05% and 0.01%). Second, the locomotion activities of L. sericata were examined at those two caffeine concentrations with treated and control male and female flies utilizing a Drosophila Activity Monitor (DAM). Treatment with 0.05% caffeine dramatically reduced locomotion, but treatment of 0.01% caffeine did not. We also compared lipid concentrations of flies: flies treated with 0.05% caffeine had a lower lipid concentration compared to flies treated with 0% and 0.01% caffeine. These results indicate that caffeine had negative effects on the longevity and locomotion activities of the pollinator L. sericata in laboratory conditions.

The version of record of this article, first published in Applied Entomology and Zoology, is available online at Publisher’s website: http://dx.doi.org/10.1007/s13355-025-00893-0