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JaLCDOI 10.18926/AMO/32771
フルテキストURL fulltext.pdf
著者 Inoue, Masanao|
抄録

For the purpo3e to determine exactly what stage of cell specialization the DNA level of erythroid cell nuclei begins to decline, the author observed the DNA level of erythroblasts in mitosis by microspectrophotometry and the DNA synthesis by flash labeling with H3-thymidine. The cell samples were obtained from the bone marrow of normal, blood-depleted and phenylhydrazine-treated animals, and the anemic animals received a mass red cell transfusion, all the animals being injected with colchicine 4 hours before obtaining the bone marrow sample. DNA level was measured on the smeared cells stained by Feulgen reaction and DNA synthesis by autoradiography on the smeared cells. Besides these, chromosome number was observed on the anemic rat erythroblasts at metaphase by air dry method. The observations indicated that the DNA level begins to decrease at polychromatic stage being accompanied by a decrease in TDH3-incorporation into DNA, reaching minimum level at orthochromatic cell both in DNA contents and synthesis. Chromosome numbers of erythroblasts of rat were irregular being distri buted between 42 to 20. The data have suggested that the DNA level of erythroblasts decreases only in the later stages of cell specialization, and at polychromatic stage the chromosome number may also decrease in rabbit at polychromatic stage by the cell division with an incomplete DNA replication. The high DNA level of the erythroblasts of rabbit, in severe anemia where most of the cells are denucleated at polychromatic and late basophilic stages, has been discussed from the view point of the insufficient DNA replication at polychromatic stages.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-04
24巻
2号
出版者 Okayama University Medical School
開始ページ 131
終了ページ 141
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4247890
NAID 120002311819
JaLCDOI 10.18926/AMO/32770
フルテキストURL fulltext.pdf
著者 Inatomi, Seiiti| Tongu, Yasumasa| Sakumoto, Daigoro| Suguri, Setsuo| Itano, Kazuo|
抄録

The body wall of the cercaria of Schistosoma japonicum is covered with a thin integument which is connected to epithel cells located under the uscle layer. On the outer and the basal surfaces of the integument are seen thin limiting membranes. In the matrix of the integument are distributed numerous dense granules, vacuoles and spines. The rootlet of the spine is attached to the basement membrane of the integument. The circular and longitudinal muscle layers, both underlying the integument, have smooth muscle fibers composed of thick and thin myofilaments. The cercaria possesses five pairs of secretion gland cells which are divided into two groups of three anterior and two posterior pairs. Both gland cells are filled with secretion balls. The tail of cercaria is likewise covered with a thin integumen t, whose structure is identically the same as the body integument. Beneath the integument are located thin circular and longitudinal muscle layers. The circular muscle cells have smooth muscle fibers, but the longitudinal muscle cells have striated muscle fibers. These muscle cells contain many large mitochondria. On observing the cross-sections of the tail at the flame cell level the arrangement of these muscle can be divided into four muscle groups and each muscle group reveals four or five muscle cells. The excretory system is well developed and has flame cells, excretory canal and bladder.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-04
24巻
2号
出版者 Okayama University Medical School
開始ページ 205
終了ページ 224
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4247893
NAID 120002311673
JaLCDOI 10.18926/AMO/32769
フルテキストURL fulltext.pdf
著者 Yasui, Shigeo|
抄録

To obtain some information of the biological action of Kankohso 101dinicotinate and Kankohso 301-nicotinate, observations were made on the binding mode of these substances with protein, chondroitin sulfate and nucleic acids and the following results were obtained; 1. Kankohso 10 I-dinicotinate binds reversively with bovine serum albumin or serum r-globulin, resulting in metachromasia. By binding with proteins the absorption maximum of the dye shifts toward the long wave length side and the absorbance decreased distinctly. The data show that there are more than one kind of binding sites and the binding with bovine serum albumin is weak in acidic solution and strong in alkaline solution. 2. Kankohso 10 I-dinicotinate produces strong metachromasia with sodium chondroitin sulfate and the color of the solution changes from violet blue to reddish violet. The absorption maximum at 592 mp. decreases without shifting its wave length ,and the shoulder appears at 555 mp. be. comes distinct peak. The strongest metachromatical changes occurs at the concentration of the chondroitinsulfate whose sulfonate radicals is equal to the molecules of Kankohso 10 I-dinicotinate. 3. Kankohso IOI-dinicotinate produces metachromasia with nucleic acid, where absorption spectrum is shifted toward long wave length and absorbance is decreased at a certain concentration. 4. Kankohso 301.nicotinate binds weakly with bovine serum albumin, the binding of which is reversible and the maximum binding number is 1.1 per molecule of albumin. Metachromasia cannot be produced by binding. Kankohso 30I.nicotinate does not bind with bovine serum γ-globulin. This compund does not produce metachromasia with sodium chondroitin sulfate but produces weak metachromasia with nucleic acid, indicating some affinity to nucleic acid.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-04
24巻
2号
出版者 Okayama University Medical School
開始ページ 185
終了ページ 204
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4195456
NAID 120002312018
JaLCDOI 10.18926/AMO/32768
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Nagao, Yutaka| Kikui, Ritsuko| Kitamura, Naoji|
抄録

Transformation of Japanese encephalitis antibody from IgM to IgG in the sera of the experimental infected chicks with Japanese encephalitis virus and transmission of IgM or IgG from hen to chicks were examined by the gel filtration on Sephadex G-200 column. The following results were obtained. 1. Titer of hemoagglutination inhibiting antibody rose on seven days after inoculation of mouse brain homogenate infected with Japanese encephalitis, and that increased rapidly after the second inoculation of Japanese encephalitis. The maximum peak of antibody titer attained on 35 days after the first inoculation, on 7 days after the second inoculation and it maintained for a period of 2 months then decreased. Viremia was detected till 6 hours after the first inoculation. 2. IgM antibody by gel filtration appeared on 7 days after the first inoculation, kept on rising, reached the peak on 35 days after the first inoculation, then decreased, and disappeared on 120 days. IgG antibody appeared about 2 weeks after the IgM antibody appearance, and the titer of IgG antibody became higher than that of IgM antibody on 35 days after the first inoculation, then decreased gradually, and showed 1 : 16 of titer of peak on 150 days by gel filtration. 3. We could obtain the chicks by fertilization from experimentally infected hen, having IgM and IgG of hemoagglutination inhibiting antibody of Japanese encephalitis. And the localization of antibodies in the sera of its chicks was determined by Sephadex G-200 gel filtration. And IgG antibody was detected in chick serum, though IgM antibody was not detected by this method.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 589
終了ページ 596
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4334579
NAID 120002312139
JaLCDOI 10.18926/AMO/32767
フルテキストURL fulltext.pdf
著者 Nishida, Shigeru| Oda, Takuzo|
抄録

1. Mitochondria isolated from hamster tumors induced by adenovirus type 12 possessed circular DNA fibers. 2. The mean value of the length of the highest frequency group of the circular DNA molecules was 4.92 ±0.38 μ. 3. Catenated dimer DNA molecules and small (less than 2 μ in length) circular DNA molecules were observed.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 551
終了ページ 557
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4258713
NAID 120002311735
JaLCDOI 10.18926/AMO/32766
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Nagao, Yutaka| Jitsunari, Fumihiko| Kikui, Ritsuko| Kitamura, Naoji|
抄録

As to trial toward the elimination of Japanese encephalitis virus in natural surroundings, pigs received inoculation of inactivated Japanese encephalitis vaccine supplemented with complete Freund's adjuvant twice at one-week interval. Effect of adjuvant supplement on the magnitude of antibody and also prevention of viremia caused by natural infection by antibody induced with vaccine were investigated. The results of this study are summarized as follows. 1. In the group of pigs inoculated with vaccine containing adjuvant, titer of hemoagglutination inhibiting and neutralizing antibodies was higher than those inoculated with vaccine alone and their high titer persisted. 2. With respect to natural infection of pigs, on August 22 when the pigs were thought to have been infected, there was observed a rise in antibody titers. And on antibody formed in those pigs inoculated with vaccine with or without adjuvant proved to be all 2-ME resistant type, whereas the antibodies produced in control group were 2-ME sensitive antibody. 3. Viremia was detected in the blood of pigs naturally infected, but it was not demonstrated pigs inoculated with vaccine supplemented with adjuvant or without adjuvant. The virus of pig blood which was inoculated into suckling mouse brain and was separated after low suckling passage mouse was supposed to be JaGAr strain from optimum hydrogen ion concentration of its hemoagglutination reaction. 4. Effect of vaccination on antibody response of pigs having maternal antibody was not recognized.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 579
終了ページ 587
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4334578
NAID 120002312045
JaLCDOI 10.18926/AMO/32765
フルテキストURL fulltext.pdf
著者 Ota, Zensuke|
抄録

The first successful electron-microscopic observation of a virus isolated from a patient with SMON was performed. The morphological and developmental characteristics of this virus suggests that this type of virus has not been isolated from humans. Hence, it is considered that the virus observed is of a new type and presumably the causative agent of SMON. The author wishes to express his profound thanks to Prof. TADASHI OFUJI for painstaking proof reading of the manuscript and also acknowledgement is due to Mr. NOBUO HAYASHI, Mr. NOBORU SAIHARA, Mr. TAKASHI NAKAMURA and Miss TOSHIYO OMIZU for their technical assistance of electron microscopy.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 573
終了ページ 577
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4258715
NAID 120002312156
JaLCDOI 10.18926/AMO/32764
フルテキストURL fulltext.pdf
著者 Chikata, Eiji|
抄録

Morphological comparison at colonial level was made on a series of established liver cell lines derived from rats fed 4-dimethylaminoazo-benzene (DAB) for various periods of days for the purpose of elucidating more accurately the differences in morphology and growth patterns among these cell lines. Colonies of each cell line produced by the single cell plating technique were compared with regard to colony size, density and piling-up of cells, atypism and pleomorphism of cells, and the migration of cells from colonies. Plating efficiency of each cell line was also compared. The cultured rat liver cells obtained from those rats fed DAB for a longer period of days showed higher plating efficiency, and increased the incidence of large-sized, dense, and piled-up colonies, of colonies consisted of cells having nuclear atypism and pleomorphism, and of irregularly margined colonies with migrating cells. The correlation between the present results and the process of DABcarcinogenesis is discussed.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 559
終了ページ 571
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4258714
NAID 120002312117
JaLCDOI 10.18926/AMO/32763
フルテキストURL fulltext.pdf
著者 Tsunemitsu, Kensuke|
抄録

We applied unidirectional MLC test to skin allografts in dogs, and examined the correlation between the strength of the MLC reaction and the survival time of skin allografts. As a result it was found that the skin allografts was rejected within 10 days when the rate of blastformation was more than 18 %. In contrast, the skin graft survived over 13 days when the rate was less than 15 %. The rate of blastformation was inversely correlated with the median survival time of skin allograft.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-12
24巻
6号
出版者 Okayama University Medical School
開始ページ 597
終了ページ 602
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4258716
NAID 120002312144
JaLCDOI 10.18926/AMO/32762
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Jitsunari, Fumihiko| Sunami, Shigeo| Hayashi, Sumiko|
抄録

Epidemiological study on the prevalence of SMON III Ibara City, Yoshii Town and Yubara Town in Okayama Prefecture was conducted and the following results were obtained. "Epidemization precession" (Die Prazession der Durchseuchung), described by Rudder which shows that ages common to this disease is shifting to younger generation as incidence rate increases, in the endemic regions was recognized in Ibara City and Yubara Town. The mode of the distribution of the interval between the primary and secondary patients in a family suggests that it corresponds to the incubation period. Distribution of the patients to the size of family is not adaptable to binomial model, but adaptable to chain binomial model, and the intra-household transmitted rate is 5 per cent, and the true intra-household transmitted rate is about two times as high as extra-household transmitted rate by the modified chain binomial model. Intra-hospital incidence among SMON patients and non-SMON patients as well as medical workers were observed at the time when the number of new patients increased in a hospital. In SMON patients, the rate of appendectomized ones was higher than that of non-SMON patients, suggesting that gastrointestinal tract has some relationship to the development of SMON disease.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 435
終了ページ 445
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254198
NAID 120002312308
JaLCDOI 10.18926/AMO/32761
フルテキストURL fulltext.pdf
著者 Ogata, Masana| Miyake, Yoshio|
抄録

For the purpose to reveal the substance as the source of offensive odor of the fish from the sea facing petroleum and petrochemical industries, analyses have been made on the sea water, industrial wastes, offensive odor fish meat and the eels kept in the sea or industrial waste for a certain period, by means of gas chromatography, infrared, and ultraviolet absorption spectrophotometries and mass-spectrometry. Observations revealed toluene as a possible main source responsible for the bad smelling of the fish. Finally, by analysing the meat extract of the fish kept in the sea water containing toluene and by the same smell as that of the offensive odor fish from the off-shore of the industries, it has been definitely confirmed that toluene is the very substance that imparts the offensive odor to fish. The activated sludge process proved to be an effective method to remove toluene from the industrial wastes. A discussion was made on the aliphatic carbohydrate as the possible source of the offensive odor of fish.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 471
終了ページ 481
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254201
NAID 120002311508
JaLCDOI 10.18926/AMO/32760
フルテキストURL fulltext.pdf
著者 Szirmai, Endre| Szantay, Janos|
抄録

Die Autoren haben 48 Patienten mit chronischer Koronarinsuffizienz mit Nitrolingual Retard Kapseln bettlagerig oder ambulant behandelt. Nach ihren Ergebnissen kann man sagen, dass Nitroglycerin in einer Langzeitform als Nitrolingual Retard Kapsel den modernen Erfordenissen der Therapie der Koronarinsufflzienz entspricht, da damit iiber einem langeren Zeitraum hinweg gleichmassig Blutspiegelwerte erzielt werden konnen. So hat man einen guten therapeutischen Erfolg, dazu einen Riickgang der Anfallhaufigkeit und eine erhohte Belastbarkeit erreicht. Der Wirkstoff hat iiber einem Zeitraum von 10-12 Stunden sich konti. nuirlich diffundiert bzw. klinisch gewirkt. Bei der Einnahme von Nitro. lingual Retard sind keine Kopfschmerzen aufgetreten. Die Vertraglichkeit des Praparateserwies sich als hut.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 489
終了ページ 491
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254203
NAID 120002312252
JaLCDOI 10.18926/AMO/32759
フルテキストURL fulltext.pdf
著者 Kawashima, Takao|
抄録

A non.specific esterase activity was demonstrated III the jejunum of rats by an azoindoxyl method. 1) Microvilli of the jejunal epithelial cells were remarkably stained in non-frozen specimens and feebly in frozen specimens. 2) The other cytoplasmic structures, i. e. mitochondria, endoplasmic reticulum, nuclear envelope, plasma membrane and multivesicular body showed a positive reaction product in frozen sections but not in non-frozen blocks.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 389
終了ページ 398
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4104312
NAID 120002311598
JaLCDOI 10.18926/AMO/32758
フルテキストURL fulltext.pdf
著者 Nishida, Shigeru|
抄録

A human fetal fibroblast strain, belonging to a group resistant to SV40 transformation, was transformed by SV40 through a multiple inoculation procedure. Two independently transformed cells were described in comparison with each other. The proportions of cells with the nuclei possessing V antigen were 2.9% at the 5th passage in one strain and 1.1 % at the 4th passage in another, and they declined gradually as frequent passages were repeated. The percentages of the transformed cells with V antigen-positive nuclei were, in both strains, quantitatively compatible with those of the cells with the nuclei full of virus particles in crystalline arrays, which were demonstrated by immunofluorescent studies and electron microscopy.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 417 417 417
終了ページ 434 434 434
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4326822
NAID 120002311811 120002311811
JaLCDOI 10.18926/AMO/32757
フルテキストURL fulltext.pdf
著者 Oda, Takuzo| Omura, Sachiko| Yamamoto, Shinichiro| Nishida, Shigeru| Hirata, Seiichi|
抄録

An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear DNA presents mainly long linear DNA derived from fragmented chromosomal DNA. In addition to this, the existence of small circular DNA molecules measuring 0.32 -1.78 μ, was confirmed. Mitochondrial DNA was mainly circular DNA, which measured 4.87 μ in the mean value of the contour lengths in the highest frequency group, and small circular DNA molecules, measuring 0.3-1.01 μ in contour length, were also found in an extremely low frequency.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 405
終了ページ 415
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254197
NAID 120002312216
JaLCDOI 10.18926/AMO/32756
フルテキストURL fulltext.pdf
著者 Oguri, Yoshio| Tasaka, Seido|
抄録

Two cases of multiple foreign bodies, i. e., duplex coins in the esophagus are reported. These foreign bodies were removed by peroral esophagoscopy successfully. Significance of roentgen-ray diagnosis is emphasized, and subtle and yet specific roentgenograms of duplex coins in the esophagus are illustrated.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 483
終了ページ 487
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254202
NAID 120002311738
JaLCDOI 10.18926/AMO/32755
フルテキストURL fulltext.pdf
著者 Szirmai, E.| Knapp, J.| Royl, P.|
抄録

Die stark eisenhaltigen Praparate Ferrlecit, Kobaltjerrlecit, Ferrojolsan, Ferrojolcamma und Irradian wurden im Siemens Unterrichtsreaktor SUR 100 des Instituts fur Kernenergetikder Universitat Struttgart und der Technischen Universitat, Berlin mit einem NeutronenfluB von ca. 5.l06n/cm2 sec bestrahlt. Es ollte dabei untersucht werden, wie stark eisenhaltige Praparate mit diesem Reaktor aktiviert werden konnen. Es wird festgestellt, daB Eisen praktisch kaum aktiviert wird. Die Ergebnisse zeigen, daB nur das Kobattjerrlecit und das Irradian starker durch Neutrosen aktiviert werden. Die hohere Aktivitat des Kobaltjerrlecit wird auf die darin enthaltenen Elemente Mangan, Kupfer und Kobalt zuruckgefuhrt, die mit thermischen Neutronen gut aktivierbar sind. Die hohere Aktivitat des Irradian geht hauptsachlich auf β-Zerfalle zuruck. Das Phosphorisotop P-32 tragt einen groβen Anteil dazu bei. De rβ-Untergrund des Irradian bei hohen Energien, der mit einer kleinen Halbwertszeitabklingt, ist durch keines der angefuhrten Elemente im Irradian erklarbar. Der Mn-56Peak im Spektrum ist gut erkennbar, durch den β-Untergrund wird er aber zu einer hoheren Energie hin verschoben.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 399
終了ページ 404
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4259622
NAID 120002311942
JaLCDOI 10.18926/AMO/32754
フルテキストURL fulltext.pdf
著者 Yumoto, Yasuhiro| Namba, Tsunko| Yamamoto, Hideo|
抄録

By assuming a three-compartment model, kinetic analysis of peripheral hyroxine distributions in various organs was represented by the alues calculated on the basis of the disappearance curve of 131I-T4 radioactivity in the serum, time dependent curve of radioactivity over the liver and urinary excretion of 131I-T4 in attempts to clarify the kinetic distribution of the thyroxine and the time dependent pool size of thyroxine in each compartment such as serum pool, liver pool, and the other pool, As a result it has been demonstrated that pool size3 of tyroxine, in the serum, liver and the other pool are enlarged in hyperthyroidism, while they are decreased in hypothyroidism in respective pools. We have recognized the reduction in the values of the liver pool size of 131I-thyroxine and 131I-thyroxine excretion into the bile, while the increase of 131I-thyroxine excretion into the urine in the cases of chronic hepatitis and cirrhosis of the liver, but the thyroxine concentration in the serum to remain within the normal level in liver diseases. As a result of the reduction in the liver pool size of 131I-thyroxine and in its uptake into the liver, the other pool size enlarges to compensate the reduction and the function of the other pool is elevated.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 457 457 457
終了ページ 470 470 470
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254200
NAID 120002311919
JaLCDOI 10.18926/AMO/32753
フルテキストURL fulltext.pdf
著者 Ohsugi, Masaki|
抄録

As a step in the elucidation of immunity of human cancer from the standpoint of homotransplantation immunity, we conducted mixed cultures of regional lymph node cells from C3H mouse isotransplanted with methylcholanthrene-induced sarcoma (MC-tumor) together with the primary culture MC-tumor cells, and observed the behaviors of these lymph node cells to the MC-tumor cells and compared the effects of these lymph node cells with lhose of normal mouse lymph node cells by counting the growth number of tumor cells and also by cinematography. As a result, it has been demonstrated that the regional lymph node cells from the mouse isotransplanted with the MC-tumor (2mm3 in size) acquire a strong antitumor activity by 14 days after the transplantation, but such antitumor activity diminishes and disappears in the terminal stage of cancer. When the number of these lymphocytes is increased, there can be observed some dosage effect, but no complete inhibition of the tumor growth can be attained. The cinematographic observations of these regional lymph node cells in the mixed culture with tumor cells reveal that lymphocytes of small to intermediate size aggregate onto the tumor cells and inhibit the movement of the latter.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1970-08
24巻
4号
出版者 Okayama University Medical School
開始ページ 447 447
終了ページ 456 456
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4254199
NAID 120002311607 120002311607
JaLCDOI 10.18926/AMO/32752
フルテキストURL fulltext.pdf
著者 Kimoto, Tetsuo| Grace, James T.|
抄録

The present report describes the findings on the infectivity of DNA partially purified from SV-40 which was propagated in the monkey kidney cells (BSC-1) in vitro and the importance of nucleic acids as oncogenic factors, particularly the induction of tumor by DNA in newborn hamsters. 593 newborn hamsters in total were used in the present experiments, and cannibalism among them posed as a serious problem. On 30 days postinoculation, very remarkable changes occurred in the liver, lung and subcutaneous areas. Cellular responses of the perivascular cells were predominant. and they were distributed in the interstitial tissues of the liver (liver cirrhosis in primates) and lung. Three hamsters of those subcutaneously inoculated with nucleic acids developed tumors and two tumors appeared in the subcutaneous tissues on 130 days postinoculation, which were identified to be the ones induced by intact SV-40 virus. Other tumors appeared in the liver, lung, intestinal ducts and abdominal surface at 126 days after subcutaneous injection. The cytological observations revealed multiple hemangiosarcoma combined with proliferation of the perivascular cells. On the other hand, cellular responses to nucleic acids were more marked by inoculation of the cell-free filtrate of BSC-1 infected by DNA than of DNA, and essential histologic findings were similar to the respo.nse to infectious DNA. Thirty-nine hamsters (30 per cent) developed tumor within about 200 days postinoculation of the filtrates. Sarcomas were common and they were confined to the subcutaneous tissues in 35 hamsters and to the peritoneum in two others by subcutaneous inoculation of the filtrates. The intestinal gland-cell carcinomas, however, could be induced at 37 and 59 days postinoculation in two hamsters of one litter (7 newborn hamsters) and in the other three newborn hamsters subcutaneous sarcomas were induced by inoculation of the same agent. These results suggest that the observation on the oncogenic capacity of nucleic acids would give us a clue to resolve the course of cancer from the view point of the infectious nucleic acid.

Amo Type Article
出版物タイトル Acta Medicinae Okayama
発行日 1966-02
20巻
1号
出版者 Okayama University Medical School
開始ページ 1
終了ページ 27
NCID AA00041342
資料タイプ 学術雑誌論文
言語 英語
論文のバージョン publisher
査読 有り
PubMed ID 4288540
NAID 120002312229