検索結果 6103 件
| 著者 | Ueda, Youki| Mori, Kyoko| Ariumi, Yasuo| Ikeda, Masanori| Kato, Nobuyuki| |
|---|---|
| 発行日 | 2011-06-17 |
| 出版物タイトル | Biochemical and Biophysical Research Communications |
| 巻 | 409巻 |
| 号 | 4号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | Miyake, Yoshiaki| Furumatsu, Takayuki| Kubota, Satoshi| Kawata, Kazumi| Ozaki, Toshifumi| Takigawa, Masaharu| |
|---|---|
| 発行日 | 2011-06-03 |
| 出版物タイトル | Biochemical and Biophysical Research Communications |
| 巻 | 409巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 方円 幸彦| |
|---|---|
| 発行日 | 1955-02-28 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 66巻 |
| 号 | 10 suppl号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 赤塚 和也| 宇野 文夫| 宇野 直道| 黒木 郁代| 山西 重機| 冨永 晃| 筒井 潔| 新居 志郎| |
|---|---|
| 発行日 | 1981-02-28 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 93巻 |
| 号 | 1-2 suppl号 |
| 資料タイプ | 学術雑誌論文 |
| JaLCDOI | 10.18926/AMO/46850 |
|---|---|
| フルテキストURL | 65_4_247.pdf |
| 著者 | Watanabe, Naomi| Shikata, Kenichi| Shikata, Yasushi| Sarai, Kei| Omori, Kazuyoshi| Kodera, Ryo| Sato, Chikage| Wada, Jun| Makino, Hirofumi| |
| 抄録 | Inflammatory processes are involved in the pathogenesis of diabetic nephropathy. The aim of this study was to clarify the role of mitogen-activated protein kinase (MAPK) pathways for induction of intercellular adhesion molecule-1 (ICAM-1) expression in glomerular endothelial cells under diabetic conditions. We examined the expression of ICAM-1 in the kidneys of experimental diabetic rats. Human glomerular endothelial cells (GE cells) were exposed to normal glucose concentration, high glucose concentration (HG), or high mannitol concentration (HM), and then the expression of the ICAM-1 protein and the phosphorylation of the 3 subfamilies of mitogen-activated protein kinase (MAPK) were determined using Western blot analysis. Next, to evaluate the involvement of MAPKs in HG- or HM-induced ICAM-1 expression, we preincubated GE cells with the inhibitors for ERK, p38 or JNK 1h prior to the application of glucose or mannitol. Expression of ICAM-1 was increased in the glomeruli of diabetic rats. Both HG and HM induced ICAM-1 expression and phosphorylation of ERK1/2, p38 and JNK in GE cells. Expression of ICAM-1 was significantly attenuated by inhibitors of ERK, p38 and JNK. We conclude that activation of ERK1/2, p38 and JNK cascades may be involved in ICAM-1 expression in glomerular endothelial cells under diabetic conditions. |
| キーワード | diabetic nephropathy ICAM-1 ERK p38 MAPK JNK |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2011-08 |
| 巻 | 65巻 |
| 号 | 4号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 247 |
| 終了ページ | 257 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2011 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 21860531 |
| Web of Science KeyUT | 000294236700005 |
| 著者 | 仁科 慎一| |
|---|---|
| 発行日 | 2011-06-30 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 舛本 明生| |
|---|---|
| 発行日 | 2011-06-30 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |
| 著者 | 森島 恒雄| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 西江 学| 岩川 和秀| 濱野 亮輔| 徳永 尚之| 常光 洋輔| 大塚 真哉| 稲垣 優| 岩垣 博巳| 合原 大博| 藤田 勲生| 村上 敬子| 友田 純| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 西江 学| 大塚 真哉| 友田 純| 藤原 敬士| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 那須 保友| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 黒田 新士| 藤原 俊哉| 白川 靖博| 山崎 泰源| 矢野 修也| 宇野 太| 田澤 大| 橋本 悠里| 渡辺 雄一| 野間 和広| 浦田 泰生| 香川 俊輔| 藤原 俊義| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 遠西 大輔| 谷本 光音| |
|---|---|
| 発行日 | 2011-08-01 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 123巻 |
| 号 | 2号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 高橋 昌造| |
|---|---|
| 発行日 | 1928-07-31 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 40巻 |
| 号 | 7号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 河村 九十九| |
|---|---|
| 発行日 | 1928-06-30 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 40巻 |
| 号 | 6号 |
| 資料タイプ | 学術雑誌論文 |
| 著者 | 岡田 正矩| |
|---|---|
| 発行日 | 1928-04-30 |
| 出版物タイトル | 岡山医学会雑誌 |
| 巻 | 40巻 |
| 号 | 4号 |
| 資料タイプ | 学術雑誌論文 |
| JaLCDOI | 10.18926/AMO/46629 |
|---|---|
| フルテキストURL | 65_3_179.pdf |
| 著者 | Teramen, Hirotake| Tsukuda, Kazunori| Tanaka, Norimitsu| Ueno, Tsuyoshi| Kubo, Takafumi| Ando, Midori| Soh, Junichi| Asano, Hiroaki| Pass, Harvery I.| Toyooka, Shinichi| Miyoshi, Shinichiro| |
| 抄録 | Suppression of p21 has been implicated in the genesis and progression of many human malignancies. DNA methylation is an important mechanism of gene silencing in human malignancies. In this study, we examined the expression status and aberrant methylaion of p21 in lung cancers and malignant pleural mesotheliomas (MPM). We used 12 small cell lung cancer (SCLC) cell lines, 13 non-small cell lung cancer (NSCLC) cell lines, 50 primary NSCLCs, 6 MPM cell lines and 10 primary MPMs. The expression and methylation of p21 was examined by reverse transcription-PCR (RT-PCR), Western blotting and methylation-specific PCR (MSP) assay. Loss of p21 protein expression was observed in 7 SCLC cell lines (58.3%), 5 NSCLC cell lines (38.5%) and 3 MPM cell lines (50%) while mRNA expression was lost in 2 SCLC cell lines (16.7%), 2 NSCLC cell lines (15.4%) and none of the MPM cell lines. Aberrant methylation of p21 was found in 8.3% of SCLC cell lines, 30.2% of NSCLCs and 6.3% of MPMs. Among primary NSCLCs, methylation in adenocarcinomas was significantly more frequent than in squamous cell carcinomas. Loss of p21 expression was frequently observed in lung cancers and MPMs and aberrant methylation was one of the mechanisms of suppression of p21, especially in NSCLCs. |
| キーワード | p21 methylation lung cancer mesothelioma |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2011-06 |
| 巻 | 65巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 179 |
| 終了ページ | 184 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2011 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 21709715 |
| Web of Science KeyUT | 000292017500004 |
| JaLCDOI | 10.18926/AMO/46627 |
|---|---|
| フルテキストURL | 65_3_163.pdf |
| 著者 | Arai, Minako| Takata, Ken| Takeda, Yoshimasa| Mizobuchi, Satoshi| Morita, Kiyoshi| |
| 抄録 | The mechanism of oxygen toxicity for central nervous system and hyperbaric oxygen (HBO) seizure has not been clarified. Noradrenergic cells in the brain may contribute to HBO seizure. In this study, we defined the activation of noradrenergic cells during HBO exposure by c-fos immunohistochemistry. Electroencephalogram electrodes were pre-implanted in all animals under general anesthesia. In HBO seizure animals, HBO was induced with 5 atm of 100% oxygen until manifestation of general tonic convulsion. HBO non-seizure animals were exposed to 25 min of HBO. Control animals were put in the chamber for 120 min without pressurization. All animals were processed for c-fos immunohistochemical staining. All animals in the HBO seizure group showed electrical discharge on EEG. In the immunohistochemistry, c-fos was increased in the A1, A2 and A6 cells of the HBO seizure group, and in the A2 and A6 cells of the HBO non-seizure group, yet was extremely low in all three cell types in the control group. These results suggest the participation of noradrenaline in HBO seizure, which can be explained by the early excitement of A1 cells due to their higher sensitivity to high blood pressure, hyperoxia, or by the post-seizure activation of all noradrenergic cells. |
| キーワード | hyperbaric oxygen seizure noradrenergic cells immunohistochemistry |
| Amo Type | Original Article |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2011-06 |
| 巻 | 65巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 163 |
| 終了ページ | 168 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2011 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 21709713 |
| Web of Science KeyUT | 000292017500002 |
| JaLCDOI | 10.18926/AMO/46626 |
|---|---|
| フルテキストURL | 65_3_151.pdf |
| 著者 | Fujiwara, Toshiyoshi| |
| 抄録 | Replication-selective tumor-specific viruses constitute a novel approach for treatment of neoplastic disease. These vectors are designed to induce virus-mediated lysis of tumor cells after selective viral propagation within the tumor. Human telomerase is highly active in more than 85ｵ of primary cancers, regardless of their tissue origins, and its activity correlates closely with human telomerase reverse transcriptase (hTERT) expression. We constructed an attenuated adenovirus 5 vector (Telomelysin, OBP-301), in which the hTERT promoter element drives expression of E1 genes. Since only tumor cells that express telomerase activity would activate this promoter, the hTERT proximal promoter would allow for preferential expression of viral genes in tumor cells, leading to selective viral replication and oncolytic cell death. Lymphatic invasion is a major route for cancer cell dissemination, and adequate treatment of locoregional lymph nodes is required for curative treatment in patients with gastrointestinal tumors. We demonstrated that intratumoral injection of Telomelysin mediates effective in vivo purging of metastatic tumor cells from regional lymph nodes. Moreover, using noninvasive whole-body imaging, we found that intratumoral injection of Telomelysin followed by regional irradiation induces a substantial antitumor effect, resulting from tumor cell-specific radiosensitization, in an orthotopic human esophageal cancer xenograft model. These results illustrate the potential of oncolytic virotherapy as a promising strategy in the management of human gastrointestinal cancer. |
| キーワード | telomerase adenovirus metastasis lymph node colorectal cancer |
| Amo Type | Review |
| 出版物タイトル | Acta Medica Okayama |
| 発行日 | 2011-06 |
| 巻 | 65巻 |
| 号 | 3号 |
| 出版者 | Okayama University Medical School |
| 開始ページ | 151 |
| 終了ページ | 162 |
| ISSN | 0386-300X |
| NCID | AA00508441 |
| 資料タイプ | 学術雑誌論文 |
| 言語 | 英語 |
| 著作権者 | CopyrightⒸ 2011 by Okayama University Medical School |
| 論文のバージョン | publisher |
| 査読 | 有り |
| PubMed ID | 21709712 |
| Web of Science KeyUT | 000292017500001 |
| 著者 | 石田 展久| |
|---|---|
| 発行日 | 2011-03-25 |
| 出版物タイトル | |
| 資料タイプ | 学位論文 |