Distribution of tumor antigen in cell fractions of adenovirus 12-induced tumor--its application to fluorescent antibody technique

Adl2-induced tumors were homogenized and fractionated by the SCHNEIDER'S method. The CF test with the serum from tumor-bearing hamsters revealed the predominant presence of T-antigen in the mitochondrial and microsomal fractions. Hence, the rabbit was immunized with the microsomal fraction of tumors, and its serum was used to prepare the fluorescent antibody to T-antigen. The direct staining of the tumor cells with so prepared fluorescent antibody gave a staining pattern similar to the indirect staining with the serum of tumor-bearing hamsters. It thus appeares possible to stain T-antigen by the direct immunofluorescent method using the serum of rabbits hyperimmunized with the microsomal fraction of Ad12-induced tumors.