細菌無細胞液による物質代謝の研究 第3篇 赤痢菌

Following up the previous experiments, with the use of extracts and acetone powder prepared from several strains of B. dysenteriae such as SM-resistant strain and SM-susceptible strain of Sh. flexneri Ⅱ, this study has been conducted in order to consider some aspects of their metabolism. The results are as follows: 1. It has been possible to obtain cell-free extracts from Sh. flexneri Ⅱ by grinding with emery powder, the extracts containing oxidases of lactate, succinate, and glycine; and also the same three oxidases have been obtained from the acetone-powder prepared from Sh. flexneri Ⅱ. Moreover, the characteristics of those oxidases obtained by the above two methods are exactly the same. 2. No marked difference can be observed in the properties of these three oxidases contained in extract from SM-resistant strain or in those from SM-susceptible strain, of Sh. flexneri Ⅱ, but there is a quantitative difference in oxygen uptake: namely, in SM-resistant strain oxygen uptake on three substrates per hour per mg of N is greater than the same in the case of SM-susceptible strain. 3. It has been confirmed that the properties of three oxidases of this bacillus, namely, oxidases of lactate, succinate, and glycine, are quite similar to the properties of those oxidases found in animals, plants, and bacteria. 4. Glycine oxidase has been extractable only from animal tissues and from and from a few kinds of bacteria, and the one that has been extracted from this bacillus possesses somewhat similar properties as those of animal tissues and other bacteria in that it is not affected by inhibitory agents of the cytochrome system and possesses a relatively narrow pH range of activity. It has been proven that glycine oxidase prepared from Sh. flexneri Ⅱ is a flavoprotein which produces glyoxylic acid and NH(3) by oxidative deamination of glycine. 5. Inhibitory action of malonate can not at all be seen on succinoxidase in resting cells of Sh. flexneri Ⅱ, while this inhibitory action is observable in the case of the acetone powder and cell-free extracts of Sh. flexneri Ⅱ. This phenomena seem to be possibly brought about by permeability of the cell membrane.