Elsevier BVActa Medica Okayama2211-7156252026Peptide nanomicelles for NIR light-dependent siRNA delivery103265ENTaufik Fatwa NurHakimDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMizukiKitamatsuDepartment of Applied Chemistry, Kindai UniversityShoumuFujimotoDepartment of Applied Chemistry, Kindai UniversityKazunoriWatanabeDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTakashiOhtsukiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityThe peptide amphiphile PA8, derived from the GAVILRR peptide, was developed as a carrier for small interfering RNA (siRNA) delivery; however, its RNA interference (RNAi) efficacy was limited owing to predominant endocytotic uptake. In this study, the RNAi efficiency of PA8 nanomicelle/siRNA complexes was enhanced by modifying the nanomicelles with the photosensitizer DY750 and the tumor-homing peptide iRGD. The conjugation of DY750 to the nanomicelles facilitated endosomal escape of the nanomicelle/siRNA complexes, enabling the cytosolic release of siRNA. Additionally, the incorporation of iRGD improved RNAi delivery efficiency in the AsPC-1 pancreatic ductal adenocarcinoma cell line. PA8-DY750-iRGD nanomicelle complexes loaded with siRNA against polo-like kinase 1 (PLK1) achieved an 80% reduction in PLK1 mRNA levels in AsPC-1 cells and a moderate 28% knockdown in NCI-N87 gastric cancer cells. Notably, no RNAi effect was observed in noncancerous 1C3D3 pancreatic cells or HEK293T kidney cells, underscoring the selectivity of this system for AsPC-1 cells. These findings highlight the potential of PA8-DY750-iRGD nanomicelle complexes as a targeted therapeutic platform for specific cancers, particularly pancreatic cancer.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2045-23221612026Liquid–liquid phase separation by caged coacervating peptides10464ENAkinariBandoDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMizukiKitamatsuDepartment of Applied Chemistry, Kindai UniversityYuukiKanazakiDepartment of Applied Chemistry, Kindai UniversityRikaTojoDepartment of Applied Chemistry, Kindai UniversityKazunoriWatanabeDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTakashiOhtsukiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityLiquid–liquid phase separation is an important biomolecular process in the formation of membraneless intracellular organelles that has inspired the development of artificial droplet systems. We developed caged coacervating peptides (CCPs) based on a histidine-rich squid beak protein sequence. The peptides were caged with a photodeprotectable (7-diethylaminocoumarin-4-yl)methoxycarbonyl group. The CCPs formed coacervates in the caged state and were partially dispersed upon blue-light irradiation. Photo-uncaging occurred rapidly, inducing coacervate dispersion. A mutant CCP with reduced π–π interactions exhibited efficient photo-dependent disassembly and enabled the encapsulation and release of a fluorescently labeled adenosine 5′-triphosphate (Bodipy-ATP) upon irradiation. These CCPs offer an efficient light-controlled approach for biomolecular encapsulation within coacervates and targeted drug delivery.No potential conflict of interest relevant to this article was reported.岡山大学算数・数学教育学会Acta Medica Okayama1341-3155312025「数学的活動」の最適化5059EN 算数・数学教育では「数学的な見方・考え方を働かせ、数学的活動を通して、『数学的に考える』」という資質・能力を育成する方向が示されている(2017)。考える力の育成を叫ぶけれども、「個別最適な学び」「協働的な学び」という指導方法・授業スタイルに眼を奪われている。数学的活動は、「数学的に考える力」を育成する中心的な学習活動であるが、数学的な活動の在り方を分析・検討することには熱心でなく、教科書どおりのきまり切った活動をさせている。数学的活動には、外的活動と内的活動があり、どんな数学的活動にすれば最適化できるのかは容易ではない。外的な操作的活動は数理的なイメージを生み出す思考を促す。内的な数学的活動は直観的な数理的イメージを内面化し言語や記号と結びつけて､算数の概念や原理を形成する思考を生成する。外的及び内的な数学的活動は､相互に関連しつつ､シンクロしながら数理的思考を促進していくので、固定的に捉えないで､弾力的・機能的に捉えることが大切である。どのような活動にすれば、数学的に考える力は創発し、生成することができるのか、数学的活動の最適化を探究することは喫緊の課題である。No potential conflict of interest relevant to this article was reported.岡山大学算数・数学教育学会Acta Medica Okayama1341-3155312025「統合的・発展的に考える力」を育成する算数科の授業 ～６年「立体の体積」の学びを通して～4249EN VUCAの時代（※）を生き抜く子どもたちに必要な資質・能力の育成が喫緊の課題とされている。算数科では永年，「統合的・発展的に考える力」を育成すべき資質・能力の中核に置いてきた。この力はVUCAの時代にも通用する力であり，子どもが「自ら課題を見付け，自ら学び，自ら考える」（確かな学力、1996）原動力になる力であると考える。しかしながら，未だそれを十分達成することはできていない。それは，「統合的・発展的に考える力」を育成するシステムが示されておらず，プロセスが確立されていないからであると考える。<br>
3年後には新学習指導要領の告示が予想される今，改めて算数科で育成すべき資質・能力の中核である「統合的・発展的に考える力」の育成に焦点を当てる。６年「立体の体積」の学びを通して，「統合的・発展的に考える力」を育成するシステムを提案する。<br>
※「VUCAの時代」…Volatility（変動性），Uncertainty（不確実性），Complexity（複雑性），Ambiguity（曖昧性）のある時代。No potential conflict of interest relevant to this article was reported.Institute of Electrical and Electronics Engineers (IEEE)Acta Medica Okayama2169-3536142026A Self-Adaptive Framework for Deploying Machine Learning Systems Without Ground-Truth Data at Runtime3030930326ENKentoFurukawaGraduate School of Information Science and Technology, Osaka UniversityHiroyukiNakagawaGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityTatsuhiroTsuchiyaGraduate School of Information Science and Technology, Osaka UniversityIn recent years, the practical application of machine learning technology has rapidly progressed, accelerating its adoption across various fields. In this context, studies into the effective operation of machine learning systems in real-world environments have become essential. In actual operational settings, the distribution of input data often changes over time, leading to a significant decline in the predictive performance of models. Additionally, the lack of ground-truth data for test data during operation can sometimes make adaptation through retraining difficult. This study proposes a framework that autonomously adapts to changes in input data distribution, even in environments where ground-truth data for test data is unavailable during operation. This framework analyzes the distribution of input data and selects the appropriate predictive model based on the state of the distribution. To ensure optimal model selection, the framework employs two complementary approaches: 1) dynamically switching between multiple pre-trained models with different feature sets according to environmental changes and 2) building ensemble models based on the distribution of the test data. These approaches enable the framework to autonomously adapt to shifts in data distribution, even in operational settings where ground-truth data is unavailable. Evaluation experiments using both simulated and real-world data assessed the predictive performance of the proposed method through metrics such as R2, RMSE, and MAE. Compared to conventional single model predictions, the proposed method consistently demonstrated higher accuracy. These results indicate that the proposed approach effectively adapts to data distribution shifts in operational environments where ground-truth data is unavailable.No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama0143-41601352026Regulation of brain-specific kinases 1 and 2 (BRSK1/2) by Ca2+/calmodulin103134ENNaoyukiWashidaMoeKataokaDepartment of Applied Chemistry and Biotechnology, Faculty of Engineering, Okayama UniversityAnna R.BrunApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityUryuTakezakiApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKoHijikawaDepartment of Applied Chemistry and Biotechnology, Faculty of Engineering, Okayama UniversityHarukiYamauchiApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySatomiOhtsukaApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasakiMagariApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityRyoMorishitaCellFree Sciences Co., Ltd.HiroshiTokumitsuApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityWe conducted a genome-wide calmodulin (CaM) interaction screening of 462 GST-fused human protein kinases to identify novel CaM-dependent protein kinases (CaMKs). In addition to known CaMKs, including myosin light chain kinases, CaMK2γ, and death-associated kinase 2, we identified the brain-specific protein kinase 2 (BRSK2, also known as SAD-A) as a novel CaM interactant. Proximity biotinylation and CaM–sepharose chromatography assays revealed that rat BRSK isoforms (BRSK1/2) interact with CaM in a Ca2+-dependent manner in vitro. We found that CaM suppresses the activation-loop phosphorylation of BRSK1 (at Thr189) and BRSK2 (at Thr175) by liver kinase B1 (LKB1), an activating kinase, in a Ca2+-dependent manner (IC50 of ∼7 µM), thereby inhibiting BRSK activation. LKB1-catalyzed phosphorylation of the catalytic domain mutant of BRSK1 (residues 1–294) at Thr189 was suppressed by the addition of Ca2+/CaM, consistent with direct CaM binding of the kinase domain, as well as wild-type BRSK1. We confirmed that the LKB1 activity was not directly suppressed by Ca2+/CaM, supporting the hypothesis that the direct interaction of Ca2+/CaM with the kinase domain blocks the phosphorylation/activation of BRSK1/2 by LKB1. The kinase activity and PP2Cα-catalyzed dephosphorylation of LKB1-phosphorylated BRSK1 were not altered by Ca2+/CaM, although it was demonstrated to bind to Ca2+/CaM like that of unphosphorylated BRSK1. This unrecognized mechanism of BRSK1/2 regulation, involving the direct role of Ca2+/CaM binding, which inhibits phosphorylation/activation by LKB1, may open a new Ca2+ signal transduction pathway in neurons.No potential conflict of interest relevant to this article was reported.岡山大学法学会Acta Medica Okayama0386-3050753-42026日本独自の違憲審査基準論の可能性586496ENT.ItoNo potential conflict of interest relevant to this article was reported.岡山大学法学会Acta Medica Okayama0386-3050753-420261789年フランス人権宣言と社会契約656588ENS.HatanoNo potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026岡山大学教育推進機構 教師教育開発センターの活動状況の概要ENNo potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026大学院共通科目『リーダーシップとSDGs』の教育モデル構築と成果分析 理論学習・ピアレビュー・省察活動によるリーダーシップ教育の新展開221235ENMamoruISHIDAFaculty of General Education and Global Studies, Okayama UniversityShinichiOTSUNEGraduate student, Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityTakuyaNAKAZAWAGraduate student, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/CTED/70371 岡山大学大学院では、博士課程人材のリーダーシップ育成に向け、共通科目『リーダーシップとSDGs』を設計・実践している。本科目はSDGsに貢献するリーダー育成に主眼を置き、理論学習・ピアレビュー・省察・グループディスカッション等の手法を組み合わせ、学生同士の学び合い・自己成長の促進を目的としている。本稿では、学部・研究科ごとに学習成果を分析し、理論に基づく省察的学びと協働的な批評活動がリーダーシップ理解や成長に有用であることを明らかにした。本研究は、学術的理論と実践的活動を織り交ぜたモデル構築と、その継続的改善の意義を示している。No potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026知的障害高等特別支援学校における SWPBS 第１層支援 ―キャンペーン方式の導入とその効果―205219ENHideakiTOKIMITSUGraduate School of Education (Professional Degree Course), Okayama UniversityYoshioMIYAZAKIFaculty of Education, Okayama UniversityMadokaKOYAMAKurashiki Kotoura Special Needs Seni or High School, Okayama Prefecture10.18926/CTED/70370 本研究は，軽度知的障害のある後期中等教育段階の公立知的障害高等特別支援学校において，SWPBS 第1層支援としてキャンペーン方式の支援を実施し，その効果検証を行った。方法としては，Ｘ学年生徒24名に対して挨拶行動の促進を狙ったキャンペーンを実施し，目標行動の生起数についてABフォローアップデザインを用いて検討した。また，Ｘ学年教員6名に対し，社会的妥当性を評価するアンケートを実施した。その結果，キャンペーンの介入直後に目標行動の生起数および目標行動に従事した生徒の割合の増加が見られ，キャンペーン終了後もベースラインと比較した増加が一定期間確認された。また，一定程度の社会的妥当性も確認できた。No potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026立憲主義に対する認識の改善を目指した高等学校公民科の授業開発研究 ―米国公民教育センター開発『我ら合衆国人民』を手がかりにして―167180ENToshinoriKUWABARAFaculty of Education, Okayama UniversityAyuhaMIYAMOTOGraduate School of Humanities and Social Sciences, Okayama University10.18926/CTED/70367 本研究は、憲法理解の基本としての立憲主義に対する認識形成を目標とする、高等学校公民科で実践可能な授業の開発を目指したものである。従来の日本の社会科教育においては、憲法理解は日本国憲法の基本原則である国民主権、基本的人権の尊重、平和主義の理解を基本としていたが、近年、そもそも憲法とは何かを理解させるために、立憲主義の概念が注目されるようになり、教科書にも記述されている。本研究は、そのような立憲主義という概念について生徒に適切に理解させることを目指した授業の提案をしようとするものである。授業計画作成にあたっては、米国の公民教育センターが開発し、長年活用されている『我ら合衆国人民（原題 We the People）』を参照し、その一部を活用し、米国の歴史的背景に基づいて作られた教材を、日本の文脈にそって改変した。No potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026中学生を対象としたサッカー試合中の身体活動量に関する研究 ～ポジションと身体・体力特性に着目して～6173ENTakaakiNATSUMEDAGraduate School of Education, Okayama UniversityMinoruADACHIOkayama University Graduate School of Education10.18926/CTED/70360 中学生34人を対象とし，身体・体力特性とサッカー試合中の身体活動量について，ポジションに着目して比較・検討した．その結果，身体・体力特性についてはポジション間に有意な差は認められなかった．歩数ではポジション間で有意差が認められ，ライトサイドバックが少なく，センターミッドフィルダーが高かった．強度別身体活動量時間の割合ではポジション間で有意差が認められ，ライトサイドバックが低く，センターミッドフィルダーが高かった．以上のことから，本研究の対象中学生ではポジション間で身体・体力特性には有意差が認められないが，サッカー試合中の身体活動量ではポジション間で差が生じることが示唆された．No potential conflict of interest relevant to this article was reported.岡山大学教育推進機構 教師教育開発センターActa Medica Okayama2186-1323162026岡山大学教育推進機構 教師教育開発センター紀要 第16号 全文（一括ダウンロード用）ENNo potential conflict of interest relevant to this article was reported.一般社団法人粉体粉末冶金協会Acta Medica Okayama0532-87997332026熱間静水圧加圧法を用いたイットリア安定化ジルコニア緻密層の低温形成5559ENKyoheiMANABEOsaka Gas Co. Ltd.MitsuakiECHIGOOsaka Gas Co. Ltd.AkiraKISHIMOTOInstitute of Academic and Research, Faculty of Environmental, Life, Natural Science and Technology, Okayama UniversityThe sintering conditions using hot isostatic press (HIP) of yttria-stabilized zirconia (YSZ) were investigated to obtain a dense YSZ layer at low sintering temperature such as 1000°C for an electrolyte of metal-supported solid oxide fuel cell. It was found that a dense YSZ pellet with relative density of 93% could be obtained under a sintering condition of 1000°C-10 hours with HIP in 195 MPa. On the other hand, in X-ray diffraction analysis of the dense YSZ pellet, peaks of the monoclinic phase were slightly detected in addition to peaks of the cubic phase. From energy dispersive X-ray spectroscopy analysis, a small amount of boron was detected in the dense YSZ pellet. It is considered that the YSZ crystalline phase transformation of cubic to monoclinic phase was occurred by the boron diffusion from the diffusion barrier coating of metal foil capsule used for the HIP.No potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024運営 : 2023年度1373ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024試験研究 : 2023年度79ENNo potential conflict of interest relevant to this article was reported.岡山大学農学部附属山陽圏フィールド科学センターActa Medica Okayama0910-8742462024序iiENNo potential conflict of interest relevant to this article was reported.American Chemical Society (ACS)Acta Medica Okayama1043-18023732026A Cysteine-Specific Cationization Strategy for Versatile Antibody Production against Intrinsically Disordered Proteins580589ENRyuiSakaguchiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAiMiyamotoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityRikakoKutsumaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTakeruMoriGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityDaichiNakashimaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMireiMasuiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTomokoHonjoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMidoriFutamiDepartment of Bioscience, Faculty of Life Science, Okayama University of ScienceMarikoMoriiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityToshiyukiOshikiDivision of Applied Chemistry, Graduate School of Natural Science and Technology, Okayama UniversityJunichiroFutamiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySeveral autoantigens relevant to the immune system, especially those targeted by autoantibodies induced by antitumor responses, tend to be rich in disordered regions and are prone to aggregation. This inherent instability presents significant challenges for the production, purification, and analysis of autoantigens in laboratory settings. Cysteine-specific cationization can effectively solubilize and purify these challenging proteins, allowing the isolation of full-length water-soluble antigens in their denatured state. The purified antigens enable accurate multiplex autoantibody assays using a suspension Luminex bead array platform. However, well-validated positive control antibodies are essential to ensuring precise clinical diagnosis. In this study, we prepared and characterized a panel of control antibodies by immunizing rabbits with cysteine-specific S-cationized antigens. The resulting antibodies predominantly recognized linear epitopes and were highly effective as quality control reagents in autoantibody array assays. Additionally, these antibodies maintained their ability to bind to their native, unmodified intracellular counterparts, highlighting the usefulness of this approach for producing antibodies against intrinsically disordered proteins. Although a modest immune response against the S-cationized modification site was observed, it remained minimal and did not affect the usefulness of the antibodies for assay validation. We propose this versatile cysteine-specific cationization platform for managing unstable proteins rich in disordered regions, supporting antigen production for diagnostics, and antibody development for research and validation purposes.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026投稿規程・奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-3227620262025 年度次世代医療機器開発人材育成プログラム BIZEN デバイスデザインコースの取り組み5968ENTsuneakiTSUZUKICenter for Innovative Clinical Medicine, Okayama University HospitalDaisukeUCHIDACenter for Innovative Clinical Medicine, Okayama University HospitalToshioKISHIMOTOOrganization for Research and Innovation Strategy, Okayama UniversityYoshinariSENGOKUCenter for Innovative Clinical Medicine, Okayama University HospitalToshioKORENAGAOrganization for Research and Innovation Strategy, Okayama UniversityYuHITOBECenter for Innovative Clinical Medicine, Okayama University HospitalYasuyukiYOSHIBAAcademic Field of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityJunSAKURAICenter for Innovative Clinical Medicine, Okayama University Hospital10.18926/interdisciplinary/70331No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-3227620262025 年度における「先進病院実習」の取り組み4957ENMizukiMORITAFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasakiMAGARIFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityJinWangFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityToyohikoWATANABEFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasatakaOITAFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNahokoHARADAFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKeisukeSHISHIDOFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/70330No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026わが国のアドバンス・ケア・プランニングにおける地域住民への動機づけと評価についてのナラティブ・レビュー3947ENTakakoHASUIJapanese Red Cross Hokkaido College of NursingNaokoNAKAYAMAKanagawa University of Human Services10.18926/interdisciplinary/70329No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026HIV 検査技術と日本における HIV 検査体制の変遷2737ENDechengWANGGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/70328No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026Shared Decision Making における患者参加の諸相と課題の考察1725ENMihoYOSHIDAGraduate School of Interdisciplinary Science and Engineering in Health Systems Okayama University10.18926/interdisciplinary/70327This paper traces the historical development of decision-making models in healthcare while exploring the meaning and practical significance of “patient participation” within the shared decision-making (SDM) framework. SDM is a recommended approach to clinical decision-making that emphasizes mutual information sharing and deliberation between physicians and patients. Traditional models often assume that patients can clearly articulate their values, preferences, and treatment goals. However, in actual clinical settings, particularly in cases of serious illness or life-threatening situations, patients frequently face emotional distress and psychological burdens, which can hinder their active participation in decision-making and the expression of their preferences. Based on SDM theory and practice reports, this study argues that SDM should not be viewed merely as a process that promotes patient choice. Even when patients choose not to actively participate and ultimately delegate decisions to healthcare providers or family members, such a choice can represent autonomous decision-making if it arises through meaningful communication and mutual understanding. This perspective calls for a more comprehensive and flexible interpretation of patient participation in SDM practice.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026介護技術論試論―高齢者介護を事例として―716ENYasuyukiYOSHIBAFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/70326In the first part of this paper, it was confirmed that the term “kaigo” (nursing care) was coined and its meaning defined during discussions on enacting social welfare legislation accompanying societal aging, as the care aspect was being “differentiated” from the “family’s health and welfare functions.” The paper also examined how the term “kaigo gijutsu”(nursing care technique) has been defined and used. In the latter part, based on the author’s own definition of “kaigo gijutsu”(nursing care technology), an attempt was made to analyze examples of technology utilization in nursing care settings, focusing on papers published in specialized welfare and nursing care technology journals. Through this preliminary study, it was shown that the author’s definition of “nursing care technology” clearly distinguishes between the means for care activities—such as welfare equipment—and the care recipients and caregivers who make use of them, and that this definition is useful for grasping the essence of challenges in nursing care settings.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026The effects of cold compresses on itching in patients with atopic dermatitis: A cross-over controlled pilot trial16ENYukiHIRAMIFormer Department of Nursing, Graduate School of Health Sciences, Okayama UniversityNahokoHARADADepartment of Nursing Science, Faculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMihoONODepartment of Nursing, Faculty of Health, Kagawa Prefectural University of Health SciencesMasahideKODACo-learning Community Healthcare Re-innovation Office, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityKiyokoFUKAIProfessor Emeritus, Okayama University, Graduate School of Nursing, The Jikei University School of Medicine10.18926/interdisciplinary/70325This cross-over controlled trial aimed to evaluate the effectiveness and safety of two types of cold compresses (towels and ice packs) in alleviating itching among patients with atopic dermatitis. The study recruited 19 participants diagnosed with atopic dermatitis and suffering from chronic itching for over 6 months. Each participant received both types of cold compress interventions. Itching sensations were assessed repeatedly using a visual analogue scale before and after the application of the cold compress. The mean and standard deviation of itching scores for the towel intervention were 16.9 ± 19.1 (baseline) and 11.4 ± 16.1 (post-application). For the ice pack intervention, the scores were 13.6 ± 14.7 (baseline) and 6.2 ± 9.8 (post-application). Although there was a reduction in mean itching scores following the application of cold compresses, the differences were not statistically significant for either intervention. Despite the lack of statistical significance, this study suggests that cold compresses, which are user-friendly and inexpensive, may safely reduce subjective itching in patients with atopic dermatitis without causing pain or discomfort. However, further research with a larger sample size is needed to confirm these findings.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322762026表紙・目次ENNo potential conflict of interest relevant to this article was reported.岡山大学理学部地球科学科Acta Medica Okayama1340-741432120262018年7月5日〜7日の西日本豪雨における広域降水特性3344ENKuranoshinKATOFaculty of Education, Okayama UniversityKengoMATSUMOTOOkayama Gakugeikan High SchoolKazuoOTANITV Setouchi Broadcasting Co., LTD.10.18926/ESR/70297 Large-scale rainfall characteristics at the heavy rainfall event around the western Japan for 5–7 July 2018 were analyzed with use of the 10-mimute precipitation data at the surface meteorological observation stations of the Japan Meteorological Agency, and so on. In this case, the area with 3 days total precipitation of near or more than 300 mm was distributed widely from northern Kyushu to Shiga and Fukui Prefectures. As in the many heavy rainfall events around Kyushu District in the mature stage of the Baiu season, contribution of the intense rainfall with more than 4 mm/10-minute (24 mm/h) attained about one third of the areal mean total precipitation. However, it is noted that the "not so intense rain" with less than 2 mm/10-minute (12 mm/h) also contributed to about one third of the huge total precipitation in the wide area. In short, this case could be characterized by the mixture of the western Japan type heavy rainfall event and the eastern Japan type one.No potential conflict of interest relevant to this article was reported.岡山大学理学部地球科学科Acta Medica Okayama1340-7414321202610分間降水量から大雨の特徴の多様性を捉える大学での授業の試み（防災気象リテラシー育成へ向けて）2131ENKuranoshinKATOFaculty of Education, Okayama University10.18926/ESR/70296 In the disaster prevention education on the heavy rainfall around Japan, it is also important to promote the meteorological literacy on the seasonal and regional differences of their rainfall characteristics such as the convective rain or stratiform rain, together with their total amount of precipitation and their occurrence frequency. As the first step toward the above purpose, the present study made a lesson practice for the university students by utilizing the 10-minute precipitation data for the four heavy rainfall events, in which the types of the heavy rainfall (although all the cases examined in the lesson are relating to the deep convective clouds) are rather different from each other, such as the differences of the rainfall intensity at the peak time, short-period variation of the rainfall intensity and the persistency of the rainfall including the "not so intense rainfall". The reports by the students seem to perceive the different features among these events briefly, but the students' attention to how long the intense rainfall with short-period variation or "not so intense rainfall" lasted was not so sufficient.No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671612026性役割と両面価値的性差別主義における就業動機との関連101120ENSuzuhaHAPPOKyokoSUMIOKA10.18926/70280No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671612026写真技術のイノベーションが創出する経済価値8599ENSatoruMIYAZAKI10.18926/70279No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671612026南オーストラリア州（アデレード）のリプロダクティブ・ヘルス＆ライツをめぐる現状と課題3146ENKeisukeSAITOSetsukoSUGANO10.18926/70276No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671612026多文化共修のためのグループワークから学生は何を感じたのか？ ― GIS ソフトを使用した地域研究授業からの一考察 ―120ENTakaoINAMORI10.18926/70274No potential conflict of interest relevant to this article was reported.岡山大学経済学会Acta Medica Okayama2433-41465732026AI 共生時代における消費者行動の再理論化―AIBCBM フレームワーク―4191ENSawutShazadigulFaculty of Humanities and Social Sciences, Okayama University10.18926/OER/70263 本研究は，AIが意思決定の前段階から介入する環境において，企業・消費者・AIの三者相互作用を体系的に説明する理論枠組みとして，Artificial Intelligence-Based Consumer Behavior Model（AIBCBM）を構築し，提示することを目的とする。まず，既存の消費者行動モデルが，アルゴリズム露出，再帰的学習ループ，AI媒介型社会的影響（Algorithmic Social Influence）といった現代的現象を十分に扱えないという決定的な理論的限界を明らかにする。そのうえで，AI共生時代における消費者行動を，「企業－AI－消費者」の三者循環構造として捉えるAIBCBMを提示する。<br>
モデル構築に際しては，複雑な現実を過度に単純化するのではなく，三者共進化構造モデル（図２），行動生成の動態を示す行動プロセスモデル（表３），概念構造モデル（図３），行動類型モデル（図４）に分離することで，理論的明瞭性と分析可能性を確保した。本研究の理論的貢献は，①System １を行動生成メカニズムとして再定義した点，②意思決定主体と権力構造を再定義した点，③消費者行動における非線形・高速フィードバックループを理論化した点，④非消費やJOMOを，幸福と主体性に根ざした戦略的行動として理論的に再定義した点，⑤消費者行動を「意思決定モデル」から「行動生成モデル」へ理論的に転換した点に集約される。さらに，本研究が提示する，アルゴリズムによる効用の向上と自律性の毀損が併存しうるという二面性は，AI時代におけるマーケティング戦略および政策設計に対して，規範的かつ理論的な新たな評価軸を提供する。AIBCBMは，これらの視座を統合する理論的プラットフォームとして，今後の実証研究に向けた基盤として機能する。とりわけ， AIBCBMは，JOMOや非消費行動を，アルゴリズム環境からの受動的撤退ではなく，AIによって構築された選択環境との距離を意図的に調整し，人間らしさ（人間としての主体性やウェルビーイング）を保持するための戦略的行動として位置づける点に独自性を有する。さらに本モデルは，AI設計（企業側）・アルゴリズム動態（AI側）・主体性とウェルビーイング（Well-being）（消費者側）を同一枠組みで接続することで，AI共生時代の実証研究と規範設計を架橋する理論的座標軸を確立する。No potential conflict of interest relevant to this article was reported.岡山大学経済学会Acta Medica Okayama2433-41465732026地域間での情報交流に関するネットワーク分析：高梁川流域圏での調査による1140ENRyoheiNakamuraNatsumiYokota10.18926/OER/70262 本稿では，岡山県の「高梁川流域連携中枢都市圏」で2014年から開催されている「高梁川流域経済成長戦略会議」における参加主体間の情報交流についてのネットワーク分析を行った。高梁川流域連携中枢都市圏（高梁川流域圏）とは，岡山県高梁川周辺に位置する現在の新見市，高梁市，総社市，早島町，倉敷市，矢掛町，井原市，浅口市，里庄町，笠岡市の10自治体が参加している連携中枢都市圏である。高梁川流域圏におけるネットワーク分析に際しては，同圏域内で展開されている事業を８つに分類し，それぞれの事業に関する参加主体間の情報交流についてアンケート調査を行った。ネットワーク指標については事業ごとに次数中心性と媒介中心性の中心性指標を，また事業別に密度，推移性，相互性を算出した。事業別にみると，観光事業についての情報交流が最も密なネットワーク構造をしており，ネットワークの視点では観光事業が高梁川流域圏内で最も勢力的に行われている事業といえる。また全事業において特定の行政主体や商工会議所をはじめとする地域経済団体等の中心性指標が全体的に大きな値をとっており，ネットワークにおいて情報交流のハブや情報の集中・分散主体として機能していることが明らかになった。分析結果を踏まえ，ネットワークの視点から高梁川流域圏の今度の地域振興について２点提言した。１つは事業によって情報交流のハブや情報の集中・分散主体を主体間で分担することによって，すべての事業で密なネットワークを築くことを目指すことである。もう１つは高梁川流域圏の情報交流ネットワークに全く参加していない主体をなくすことで，全体的に密なネットワークを目指すことである。No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama1478-811X2412026MMP-3 cleavage of Lamin A induces pro-migratory nuclear deformity, nucleophagy, and their autophagic secretion with extracellular vesicles in metastatic cancer146ENTakanoriEguchiDepartment of Dental Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityEman A.TahaDepartment of Biochemistry, Faculty of Science, Ain Shams UniversityKeisukeNakanoDepartment of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityVikasTiwariCouncil of Scientific & Industrial Research-Indian Institute of Toxicological ResearchKatsukiTakebeDepartment of Dental Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTomohiroInoueDepartment of Dental Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityLiziXingDepartment of Dental Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityChiharuSogawaDepartment of Food and Health Sciences, Faculty of Environmental Studies, Hiroshima Institute of TechnologyKuniakiOkamotoDepartment of Dental Pharmacology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityStuart K.CalderwoodDivision of Molecular and Cellular Biology, Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical SchoolMatrix metalloproteinases (MMPs) are a family of zinc-dependent proteinases that cleave a plethora of substrates, including components of the extracellular matrix and cell-surface-associated proteins, as well as intracellular targets. MMPs have also been found in extracellular vesicles (EVs), such as exosomes. MMP-3 promotes tumor growth, epithelial-to-mesenchymal transition, genome instability, migration, invasion, and metastasis of cancer cells, and nuclear MMP-3 controls gene transcription. Intranuclear proteolysis by MMPs may significantly alter cancer progression. However, the nuclear substrates of MMP-3 have not been well investigated. In this study, we performed proteomic analyses to identify the nuclear substrates and EV proteins regulated by MMP-3. While rabidly metastatic colon cancer (LuM1) three-dimensionally cultured tumoroids secreted EVs containing 30 protein types, including Lamin A (LMNA), MMP-3, fibronectin (FN1), HSPA8 (Hsc70), β-actin (ACTB), and vimentin (VIM), CRISPR/Cas9-based knockout of MMP-3 reduced the secretion of these proteins in EVs. Notably, EV-bound cleaved Lamin secretion was confirmed by immunoelectron microscopy. Also, MMP-3 formed proteolytic dimers via its hemopexin-like repeat domains in nuclei. Many nuclear MMP-3-binding proteins, including Lamin A/C, histones, topoisomerases, and hnRNPs, were screened by co-immunoprecipitation followed by proteomics. Proteolytic MMP-3 overexpression generated a C-terminal 30-kDa fragment of Lamin A, whose cleavage site was defined via structural analysis. MMP-3 digestion of Lamin A induced nuclear deformity (atypia) required for cell migration in confined space. The cleaved Lamin A and MMP-3 were transported with autophagosomes (LC3B+), nucleophagosomes, and amphisomes (CD63 + LC3B+) and co-secreted with EVs. Proteolytic MMP-3 also induced nuclear speckles of Lamin A, suggesting their roles in transcription and splicing. Clinical analysis revealed that high expressions of MMP3 and LMNA were significantly seen in head and neck squamous cell carcinoma (HNSC) than in the other 16 cancer types, and predicted poor prognosis of patients suffering from HNSC, pancreatic, rectum and lung adenocarcinomas at specific stages. Immunohistochemistry revealed that nuclear MMP-3 and cleaved Lamin were significantly higher expressed in stage IV metastatic HNSC cases than in stage I non-metastatic cases. Taken together, MMP3-cleavage of Lamin A induces nuclear deformity, nucleophagy, and their autophagic co-secretion with EVs in metastatic cancer. Also, high expression of MMP-3 and secretion of Lamin A can predict poor prognosis in multiple cancer types at specific stages.No potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama2076-34171652026Concentration-Dependent Synergistic Interfacial Interactions Between Multifunctional Acrylate and Silane Coupling Agents in an Organic–Inorganic Nanohybrid Material2339ENYukinoriMaruoDepartment of Prosthodontics, Okayama UniversityKumikoYoshiharaHealth Research Institute, National Institute of Advanced Industrial Science and TechnologyMasaoIrieDepartment of Biomaterials, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNoriyukiNagaokaAdvanced Research Center for Oral and Craniofacial Sciences, Dental School, Okayama UniversityNaokiKodamaDepartment of Prosthodontics, Okayama UniversityMaiYoshizaneDepartment of Occlusal and Oral Functional Rehabilitation, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityKentaroAkiyamaDepartment of Occlusal and Oral Functional Rehabilitation, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversitySynergistic effects of a multifunctional acrylate and a long-chain silane coupling agent were investigated in an organic–inorganic nanohybrid material. We tested the bond strength of nanohybrid composites treated with experimental primers containing silane coupling agents—3-methacryloxypropyl trimethoxysilane (γ-MPTS) or 8-methacryloxyoctyl trimethoxysilane (8-MOTS)—with or without multifunctional acrylates—trimethylolpropane triacrylate (A-TMPT) or dipentaerythritol hexaacrylate (A-DPH). Shear bond strength was evaluated after 24 h of water storage at 37 °C. Untreated control and silane-only groups exhibited low shear bond strengths (e.g., control: 2.4 ± 2.0 MPa) and failed exclusively at the adhesive interface. While addition of A-TMPT did not significantly improve bond strength, addition of A-DPH produced significantly higher shear bond strengths. Highest strength was achieved with 30% 8-MOTS and A-DPH (22.4 ± 6.1 MPa), followed by 20% γ-MPTS and A-DPH (19.0 ± 7.0 MPa), and A-DPH groups produced cohesive failures. Regardless of the silane used (γ-MPTS or 8-MOTS), incorporating A-DPH in the primer consistently yielded superior bond strengths, indicating a promising strategy for improved adhesion for such nanohybrid systems. These findings provide new insights into optimizing resin–filler interfacial interactions and may contribute to the development of restorative materials with improved long-term clinical durability.No potential conflict of interest relevant to this article was reported.American Chemical Society (ACS)Acta Medica Okayama0022-26236952026Discovery of Thermal Sensitizers That Inhibit Heat-Induced SAFB Granule Formation59445955ENYujiFurutaniFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNatsukiShimasakiFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityRikoYamadaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTakashiOhtsukiFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazunoriWatanabeFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityHyperthermia is a minimally invasive cancer treatment based on heat stress-induced apoptosis. Its therapeutic efficacy, however, is often limited by tumor heterogeneity and acquired thermotolerance. Therefore, combination strategies involving hyperthermia and chemotherapy have been developed to enhance the therapeutic efficacy. Previously, we showed that SB366791 enhanced heat-induced apoptosis by inhibiting heat stress-induced scaffold attachment factor B (SAFB) granule formation, although its proapoptotic activity was insufficient. Therefore, we screened to identify novel compounds that enhance heat-induced apoptosis by suppressing SAFB granule formation. We identified four hit compounds that inhibited SAFB granule formation, all exhibiting thermal enhancement ratios > 1.0─that significantly enhanced heat-induced apoptosis efficiency. Additionally, the tumor volume in mice treated with a combination of Z19024498 and hyperthermia was significantly smaller than that in mice treated with hyperthermia or Z19024498. These results indicate that the identified compounds, specifically Z19024498, have potential as thermal sensitizers for hyperthermia therapy.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama1613-681021502025Collagen Signaling via DDR1 Exacerbates Barriers to Macromolecular Drug Delivery in a 3D Model of Pancreatic Cancer Fibrosise06926ENMayuOhiraDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMoeKitamuraDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityHiroyoIwasakiDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityHarukoOhta‐OkanoDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityHiyoriTsujiiDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityReikaNakamuraDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTakuyaNakazawaDepartment of Pharmaceutical Biomedicine, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAkihiroNishiguchiBiomaterials Field, Research Center for Macromolecules and Biomaterials, National Institute for Materials ScienceMasayaYamamotoDepartment of Materials Processing, Graduate School of Engineering, Tohoku UniversityKensukeOsadaDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, National Institutes for Quantum Sciences and Technology (QST)ShinichiToyookaDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityHoracioCabralDepartment of Bioengineering, Graduate School of Engineering, The University of TokyoAtsushiMasamuneDivision of Gastroenterology, Graduate School of Medicine, Tohoku UniversityMitsunobu R.KanoDepartment of Pharmaceutical Biomedicine, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityHiroyoshi Y.TanakaDepartment of Pharmaceutical Biomedicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityFibrosis is a significant barrier to drug delivery in pancreatic ductal adenocarcinoma (PDAC) and contributes to its dismal prognosis. Pancreatic stellate cells (PSCs) drive fibrosis by excessively secreting extracellular matrix proteins such as collagen I. Collagen I is thought to physically obstruct the delivery of macromolecules, such as albumin, antibodies, and nanomedicines. Apart from its structural role, collagen signals through dedicated cell surface receptors, such as the discoidin domain receptors (DDR) 1/2. However, whether and how collagen signaling contributes to fibrotic barrier generation remains uncharacterized. Here, a 3D culture model of PDAC fibrosis constructed from patient PSCs is used to assess the contribution of DDR1/2-mediated collagen signaling. DDR1/2 inhibition diminishes collagen I expression in PSCs to enhance macromolecular delivery. Moreover, MEK inhibitors exacerbate the fibrotic barrier by up-regulating collagen I, an effect reversed by inhibiting DDR1/2. Through isoform-specific targeting, inhibiting DDR1, but not DDR2, is shown to be effective. Downstream of DDR, the involvement of the PI3K/AKT/mTOR pathway is demonstrated, particularly alternative mTOR complexes involving MEAK7 and GIT1. Altogether, the results show in vitro that DDR1-mediated collagen signaling exacerbates the fibrotic barrier and may be targeted to enhance macromolecular drug delivery in PDAC.No potential conflict of interest relevant to this article was reported.岡山大学文学部Acta Medica Okayama0285-4864782025文学部教員研究活動一覧（2024年度）6377ENNo potential conflict of interest relevant to this article was reported.岡山大学文学部Acta Medica Okayama0285-4864782025可能表現としての「可能だ」「不可能だ」5162ENKazuhitoMIYAZAKI10.18926/okadai-bun-kiyou/70220No potential conflict of interest relevant to this article was reported.岡山大学文学部Acta Medica Okayama0285-4864782025非大都市圏小規模自治体の持続的発展と地域的対応の特徴111ENHirofumiKITAGAWA10.18926/okadai-bun-kiyou/70216No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231912026概念型カリキュラムに基づく平和教育単元の開発と実践 ― 声をきく，つくる，とどける ―197213ENYuichiMIYAMOTOFaculty of Education, Okayama UniversityYudaiMakabeTeacher at an International SchoolShunSATOEduca & Quest Inc.TomochikaOSHIROGraduate School of Humanities and Social Sciences, Hiroshima UniversityMikaMATSUYAMAKyodo Public Relations Co., Ltd.10.18926/bgeou/70208 本稿は,生徒たちが自己や社会にひかれた境界線への理解を深め(境界線の「上に立つ」),境界線を「別様に引き直す」可能性を追究するというコンセプトで作られたカリキュラム開発プロジェクトのうち，平和教育カリキュラムの開発と実践の成果をまとめたものである。他者存在との共生と協調に関わる概念を，「声」というメタファーに集約させて6つ選定した。生徒たちが,世界に引かれた境界線をどのように理解し,どのように自らの生活の中の境界線を捉えなおそうとしたかについて分析した。カリキュラム構成上の意義と課題に関して，学習した概念の生活認識への転用の困難が明らかとなり,カリキュラムの中に概念の省察と吟味を重点的に行う活動を入れることの重要性が明らかとなった。No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231912026高校特別支援教育コーディネーターにおける小児がん患児支援に関する教育施策への理解と役割意識177185ENYiwenCHENThe Joint Graduate School (Ph.D. Program) in Science of School Education Hyogo University of Teacher EducationMunehisaYOSHITOSHIFaculty of Education, Okayama University10.18926/bgeou/70206 本研究の目的は，小児がんを経験した高校生に対する特別支援教育コーディネーターの支援意識を明らかにすることである。自治体の研修講座に参加した59名を対象に質問紙調査を実施し，23名から回答を得た。量的データは単純集計，自由記述は質的記述的分析により分析した。結果として，小児がん患児に関連する新たな教育施策，特に同時双方向型授業に関する認知度が低く，制度が十分に周知されていない実態が明らかになった。医療関係者との連携については，高い役割意識が示された一方で，病院訪問はあまり重視されていなかった。また，学級通信や教材準備などは必ずしも特別支援教育コーディネーターの役割とは認識されていなかった。自由記述からは，ICT を活用した支援への積極的な姿勢が示されたものの，中学校との連携における情報共有や業務負担に関する課題がみられた。今後，連携に関するガイドラインの整備や情報共有の強化が求められることを指摘した。No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231912026教職員研修の高度化に果たす大学の役割と課題 ― NITS 岡山大学センターの活動を通して ―116ENAtsushiTAKASEFaculty of Education, Okayama UniversityAkikoTSURUMIOkayama University KindergartenChiyoKUROSUMIOkayama University KindergartenTetsuoKIYOTAFaculty of Education, Okayama UniversityYoshihikoINADAFaculty of Education, Okayama UniversityAiMATSUURAFaculty of Education, Okayama UniversityKoujiMIYAMOTOFaculty of Education, Okayama UniversityMutsumiMATSUEDAFaculty of Education, Okayama UniversityAikoTSUSHIMAFaculty of Education, Okayama UniversityYoshioMIYAZAKIFaculty of Education, Okayama UniversityToshiyaTAKEMOTOOkayama University School for Special Needs EducationYokoSAWATANIOkayama University School for Special Needs EducationKazuakiKAJIIFaculty of Education, Okayama UniversityMakikoKANAGAWAFaculty of Education, Okayama University10.18926/bgeou/70192 本論文では，専門職としての教職員の学びを保障する研修のあり方を検討し，とくに大学が果たす役割と課題について考察する。教職員の学びは教育委員会での研修，勤務校園での研修，教職大学院での学修，研究団体での研修などにおいて展開される。大学が開発・実施する研修はこれらとどう関連し，どのような特色をもつか。独立行政法人教職員支援機構（以下，NITS）岡山大学センターの活動を通して検討する。そして，大学は教職員の学びのニーズに応える側にあるだけでなく，教職員の学びを再構成し，自律的協働的な学びを支援・促進していく側としての役割を果たすものであることを考察する。また，大学おけるアウトカム重視の研修開発の必要性を指摘し，今後の取組の課題として示す。No potential conflict of interest relevant to this article was reported.Japan Stroke SocietyActa Medica Okayama0912-07264762025頭蓋頚椎移行部動静脈瘻の開頭手術時における上腕動脈穿刺による脳血管撮影の有用性410412ENYuichiHirataDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesMasafumiHiramatsuDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesSusumuSasadaDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesJuntaroFujitaDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesYutaSotomeDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesMasatoKawakamiDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesRyuKimuraDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesYukiEbisudaniDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesKyoheiKinDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesJunHarumaDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesKenjiSugiuDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesShotaTanakaDepartment of Neurological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences頭蓋頚椎移行部動静脈瘻の手術では，異常血管の確認のため術中血管撮影が重要であるが，体位変換時のsheathの固定や撮影血管へのカテーテルの誘導が困難である．今回，頭蓋頚椎移行部動静脈瘻の開頭手術において上腕動脈穿刺によりultra-long sheathを鎖骨下動脈に留置して椎骨動脈撮影（vertebral artery angiography: VAG）を行う方法の有用性を報告する．当院で本血管撮影を施行した症例の患者背景や合併症を評価した．企図した4例，5血管でVAGを施行でき，合併症も認めなかった．体位変換も腹臥位における穿刺部の観察も簡便であり，カテーテルの操作性にも優れ，VAGを容易に施行することができた．また，管球を頭側から移動する際の術野との干渉も最小限に抑えられた．本方法は利点が多く，有用な血管撮影方法であり，頭蓋頚椎移行部動静脈瘻の手術における一助となり得ると考える．No potential conflict of interest relevant to this article was reported.大阪府保険医協会Acta Medica Okayama547132026『光が見える』人工網膜の可能性 ― 有機色素分子を部材とする世界初の医療機器「光電変換色素薄膜型人工網膜OUReP」1321ENToshihikoMatsuoTetsuyaUchidaHiroshiIshikane 網膜色素変性や加齢黄斑変性では、光を細胞膜電位に変換する網膜視細胞が死んでいるが、視神経として脳に連絡する神経節細胞は生き残っている。人工網膜は視細胞を代替する人工物で、光を受け電流を出力する電極アレイ型が主流であるが、電流は拡散するため解像度向上が難しい。そこで人工網膜の解像度向上を目指して、光を電位差に変換する光電変換色素分子を絶縁体のポリエチレン薄膜表面に共有結合した光電変換色素薄膜型の人工網膜OURePを開発してきた。この人工網膜OURePは光受容と電位出力の一体型で外部起電力は不要、手術では薄膜を鋏で切って眼内に植込む大きさを自由に選べる。使い捨てインジェクタを使って薄膜を丸め眼球の網膜下に硝子体手術で植込み、網膜下に植込んだ人工網膜OURePは光を受けて電位差を出力し隣接する網膜組織の神経細胞の活動電位を誘発する。クリーンルームで製造品質管理を行い、安全性と有効性を証明して、医師主導治験を準備している。今後、日本の国民皆保険が維持できるよう比較安価な適正価格の人工網膜治療を提供したい。No potential conflict of interest relevant to this article was reported.岡山大学教育推進機構Acta Medica Okayama1881-595232026オンラインによるボランティア日本語教室における参加者間のインターアクションの分析 ―地域型日本語教育の実現に向けて―3140ENMiwaSUESHIGEInstitute for Promotion of Education and Campus Life, Okayama University10.18926/70110 本研究では，地域型日本語教育のモデル構築に向け，地域型の特徴を有する教室内での支援者および参加者の発話や教授行動の傾向を明らかにすべく，外国語相互作用分析システムを用い，オンラインによるボランティア教室におけるインターアクションの分析を行った。分析の結果，（1）支援者の平均発話数が学習者より多いこと，（2）支援者の発話における間接的行動の割合が直接的行動よりもやや高いことが示された。「おしゃべり型の教育」では，学習者が伝えたいことを言語化していくプロセスの中で，間接的行動の下位分類の「質問」「学習者の意図の利用」「学習者の回答の繰り返し」等を支援者が積極的に使用し支援していることが分かった。No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama2590-1230272025Inscribed-type spherical speed reducer with uniform reduction ratio in all directions106742ENSeiyaNaramuraFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityKoichiTonegawaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySoShimookaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityTomoakiYanoFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityAkioGofukuOkayama Prefectural UniversityNagayoshiKasashimaNational Institute of Advanced Industrial Science and TechnologyTetsushiKamegawaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityA spherical motor is an actuator that can generate rotational motion about all three orthogonal axes. However, it is difficult to obtain high output torque from most electromagnetic spherical motors, primarily due to limitations inherent in electromagnetic actuators, such as restricted magnetic force and thermal constraints. Since its torque cannot be increased using planar gears, spherical speed reducers that transmit rotational torque along three orthogonal axes through sphere-to-sphere contact are required. One major limitation of conventional spherical speed reducers is that their size increases significantly as the reduction ratio becomes higher. To address this issue, we propose a novel inscribed-type spherical speed reducer, in which the deceleration mechanism is integrated within the output sphere. This configuration enables a more compact design, reducing the overall size to approximately half that of conventional designs. To predict the angular velocity and transmitted torque, theoretical models for the rotation and torque transmission of the speed reducer were developed. According to the proposed model, the reduction ratio of the spherical speed reducer is 1/3. To verify the validity of these models, experiments were conducted to measure angular velocity and torque. The theoretical results agreed well with the experimental results. In addition, the theoretical torque exhibited an average relative error of 1.63 % compared to the experimental result. Therefore, it was confirmed that the rotation and torque transmission models were valid. These results demonstrate that a reduction ratio can be obtained in all directions of the 3-DOF of the spherical speed reducer, unlike conventional 1-DOF reducers.No potential conflict of interest relevant to this article was reported.岡山大学文明動態学研究所Acta Medica Okayama2436-832652026大知聖子著『計量的分析を用いた北魏史研究』279285ENHirokazuHASEGAWA書評 (Book Review)10.18926/70065No potential conflict of interest relevant to this article was reported.岡山大学文明動態学研究所Acta Medica Okayama2436-832652026島根県猪目洞窟遺跡出土人骨の年代・食性・遺伝的特徴2039ENHideakiKANZAWA-KIRIYAMADivision of Human Evolution, Paleontology and Anthropology, National Museum of Nature and Science, Tsukuba City, Ibaraki PrefectureMaiTAKIGAMIDivision of Human Evolution, Paleontology and Anthropology, National Museum of Nature and Science, Tsukuba City, Ibaraki PrefectureTsuneoKAKUDADepartment of Legal Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of YamanashiLeoSPEIDELCenter for Interdisciplinary Theoretical and Mathematical Sciences, RIKENGarrettHELLENTHALDepartment of Genetics, Evolution and Environment, University College London Genetics Institute (UGI), University College LondonNancyBIRDDepartment of Genetics, Evolution and Environment, University College London Genetics Institute (UGI), University College LondonYousukeKAWAIGenome Medical Science Project, National Institute of Global Health and Medicine, National Institute for Health SecurityNCBN Controls WGS ConsortiumMinoruSAKAMOTONational Museum of Japanese HistoryYuichiKAMEDADivision of Human Evolution, Paleontology and Anthropology, National Museum of Nature and Science, Tsukuba City, Ibaraki PrefectureNoboruADACHIDepartment of Legal Medicine, Interdisciplinary Graduate School of Medicine and Engineering, University of YamanashiKen-ichiSHINODANational Museum of Nature and ScienceNaruyaSAITOUNational Institute of GeneticsTatsuhikoHAMADAResearch Institute for the Dynamics of Civilizations, Okayama University論文 (Research article)10.18926/70052This paper reports on the integrative research findings of the human bones excavated from the Inome Cave Site in Shimane Prefecture, based on dietary estimation using carbon and nitrogen isotope analysis, radiocarbon dating, and whole genome analysis. The dates of the analyzed human bones span a wide range, from the Middle to Late Kofun period, the Nara period to the Early Heian period, and the Middle to Late Heian period, indicating that the Inome Cave Site was continuously used as a burial place. Dietary habits were a mixture of C3 resources (C3 plants and terrestrial animals that consumed C3 plants) and marine resources, with individual variations in the intake of marine and terrestrial resources. A correlation was observed between differences in dietary habits and individual variations in the Jomon ratio in the nuclear genome, with individuals who consumed higher amounts of marine resources tending to have a higher Jomon ratio. This suggests that individuals with different backgrounds were buried in the same site due to interactions with surrounding settlements.No potential conflict of interest relevant to this article was reported.岡山大学文明動態学研究所Acta Medica Okayama2436-832652026日本における「ロシアかぜ」流行の社会史的分析 ―1889 -1891 年パンデミックと日本社会―119ENAtsushiKAWAUCHIUehiro Disaster Risk Reduction Research Division, International Research Institute of Disaster Science (IRIDeS), TOHOKU UNIVERSITY論文 (Research article)10.18926/70051This study offers a social-historical analysis of the “Russian flu” pandemic in Japan (1889–1891). Due to scarce statistical data, the study relies primarily on contemporary newspapers and magazines. It identifies two distinct epidemic waves: the first in spring-summer 1890, and the second from late 1890 to spring 1891. The first wave, though widespread, was overshadowed by a concurrent cholera epidemic and caused relatively few deaths, whereas the second wave was far more lethal and generated widespread fear. At the time, influenza remained an “unknown disease”, with unclear etiology and no established treatments. People responded with diverse measures, from purchasing patent medicines and using folk remedies to symbolic practices such as "disease naming" (osome-kaze). The crisis also renewed attention to historical records of earlier influenza-like epidemics in Japan. In contrast, by the time of the later “Spanish flu” pandemic, advances in bacteriology had already rendered influenza a medically defined disease. This comparison highlights how shifting medical knowledge shaped societal responses. The findings not only corroborate previous excess-mortality analyses but also provide new historical insights into how societies have historically confronted pandemics.No potential conflict of interest relevant to this article was reported.眼科臨床紀要会Acta Medica Okayama1882-517619022026斜視の遺伝子研究113125ENToshihikoMatsuoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University共同性斜視は遺伝要因と環境要因からなる多因子疾患で、内斜視と外斜視に大別される。遺伝要因は家族歴、一卵性双生児の表現型一致率から推定され、環境要因には妊娠・分娩時の低酸素状態がある。一方、遺伝要因がある非共同性（麻痺性）斜視として上斜筋腱低形成を呈する特発性上斜筋麻痺がある。遺伝統計学の連鎖解析を使って、内斜視と外斜視の小家系群で4番染色体MGST2を疾患感受性遺伝子候補と同定し、MGST2ノックアウトマウスを作成した。小動物用MRIで解析すると、そのホモ接合体では野生型と比べて眼球形状が有意に横長で体積が大きいことを見出した。次いで遺伝統計学別法の全ゲノム関連解析を内斜視、外斜視、特発性上斜筋麻痺を対象として行った。Infinium Asian Screening Array-24 v1.0でSNPを決めた内斜視253検体、外斜視356検体、上斜筋麻痺102検体を疾患群とした。対照集団としては、バイオバンクジャパン (BBJ) の疾患群とは違うアレイ(OmniExpress)でSNPを決めた182,476検体「BBJ (180K)」、疾患群と同じアレイでSNPを決めたBBJの53409検体「BBJ (ASA)」および長浜コホート3570検体を使った。３対照集団との比較で共通して検出された遺伝子は、上斜筋麻痺群で神経細胞移動に関与するDAB1であった。最も大きい対照集団「BBJ (180K)」との比較では内斜視、外斜視、上斜筋麻痺を含む疾患群全体で眼発生に関与するRARB (retinoic acid receptor β) が検出された。斜視関連遺伝子は眼球形態に関与する可能性がある。特発性上斜筋麻痺は共同性斜視とは独立した疾患と理解されるが、共通の遺伝基盤もあるかもしれない。No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671602025日本語版 The Passive Aggression Scaleの作成および信頼性と妥当性の検討245260ENAyakaSHIGETOUYoung-OkLimKyung-HyunSuhKyokoSUMIOKA10.18926/70031No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671602025漢語接尾辞「派」による派生語について― 前接語が固有名詞の場合 ―157168ENXUELI10.18926/70026No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671602025ライプニッツの「人間学」―『定義集』の叙述を中心に―3150ENTsuyoshiMATSUDA10.18926/70019No potential conflict of interest relevant to this article was reported.American Association for the Advancement of Science (AAAS)Acta Medica Okayama2375-254811442025Structural insights into the divergent evolution of a photosystem I supercomplex in Euglena graciliseaea6241ENKojiKatoResearch Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYoshikiNakajimaResearch Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityRunaSakamotoResearch Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityMinoruKumazawaInstitute of Low Temperature Science, Hokkaido UniversityKentaroIfukuGraduate School of Agriculture, Kyoto UniversityTakahiroIshikawaInstitute of Agricultural and Life Sciences, Academic Assembly, Shimane UniversityJian-RenShenResearch Institute for Interdisciplinary Science, Advanced Research Field, and Graduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityAtsushiTakabayashiInstitute of Low Temperature Science, Hokkaido UniversityRyoNagaoFaculty of Agriculture, Shizuoka UniversityPhotosystem I (PSI) forms supercomplexes with light-harvesting complexes (LHCs) to perform oxygenic photosynthesis. Here, we report a 2.82-angstrom cryo–electron microscopy structure of the PSI-LHCI supercomplex from Euglena gracilis, a eukaryotic alga with secondary green alga-derived plastids. The structure reveals a PSI monomer core with eight subunits and 13 asymmetrically arranged LHCI proteins. Euglena LHCIs bind diadinoxanthin, which is one of the carotenoids typically associated with red-lineage LHCs and is not present in the canonical LHCI belt found in green-lineage PSI-LHCI structures. Phylogenetic analysis shows that the Euglena LHCIs originated from LHCII-related clades rather than from the green-lineage LHCI group and that the nuclear-encoded PSI subunit PsaD likely originated from cyanobacteria via horizontal gene transfer. These observations indicate a mosaic origin of the Euglena PSI-LHCI. Our findings uncover a noncanonical light-harvesting architecture and highlight the structural and evolutionary plasticity of photosynthetic systems, illustrating how endosymbiotic acquisition and lineage-specific adaptation shape divergent light-harvesting strategies.No potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama2079-63741612026Magnetic Detection of Cancer Cells Using Tumor-Homing Peptide-Modified Magnetic Nanoparticles45ENShengliZhouDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYujiFurutaniDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKeiYamashitaDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySakuyaKakoDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazunoriWatanabeDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityToshihikoKiwaDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTakashiOhtsukiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMagnetic nanoparticles (MNPs) provide a platform for target detection because of their magnetic responsiveness to alternating magnetic fields (AMFs). We developed a detection method using MNPs modified with tumor-homing peptides (THPs), PL1 and PL3, which selectively bind to protein components enriched in malignant tissues. THP-MNPs were synthesized using maleimide-PEG-NHS linkers and characterized using transmission electron microscopy. Human glioblastoma cancer U87MG and normal tissue-derived HEK293 cells were incubated with THP-MNPs, and the magnetic signals were measured using a high-temperature superconducting quantum interference device (SQUID) magnetometer under an AMF (1.06 kHz). Dark-field microscopy confirmed the preferential binding of THP-MNPs to U87MG cells. In the absence of cells, THP-MNPs exhibited AMF-dependent signal enhancement, which correlated with particle size reduction due to THP release. This increase was completely suppressed in the presence of U87MG cells, indicating a strong THP-mediated interaction. PL3-MNPs exhibited superior discrimination between malignant and non-malignant cells. These results demonstrate that SQUID-based magnetic measurements using THP-MNPs enable rapid and label-free cancer cell detection.No potential conflict of interest relevant to this article was reported.American Chemical Society (ACS)Acta Medica Okayama0006-296064202025Characterization of Autonomous and Ca2+/Calmodulin-Dependent Activities of CaMKK Isoforms In Vitro and in Mouse Tissues43094317ENSatomiOhtsukaApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYerunChenApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasakiMagariApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTeruhikoIshikawaDepartment of Science Education, Graduate School of Education, Okayama UniversityHiroyukiSakagamiDepartment of Anatomy, Kitasato University School of MedicineFutoshiSuizuClinical Examination Department, Kagawa Prefectural University of Health SciencesHiroshiTokumitsuApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityCa2+/CaM-dependent protein kinase kinase (CaMKK) phosphorylates and activates downstream kinases, including CaMKI, CaMKIV, PKB, and AMPK, regulating various cellular functions such as neuronal morphogenesis, metabolic control, and pathophysiological pathways, such as cancer progression. CaMKKα/1 is tightly regulated by an autoinhibitory mechanism. CaMKKβ/2 activity is highly Ca2+/CaM-independent (autonomous activity) in vitro and Ca2+/CaM-dependent in cultured cells. Whether these two activity states of CaMKKβ/2 exist in vivo and the detailed regulatory mechanisms for the transition of both activity states remain unclear due to the difficulty in distinguishing the two activity states. In this study, we detected Ca2+-dependent and autonomous CaMKK activity in HeLa cells and successfully separated both activity states of CaMKKβ/2 in mouse brain and testis extracts using a recently developed CaMKK inhibitor (TIM-063)-coupled sepharose, which binds to the catalytic domain in the active state but not in the autoinhibited state. Furthermore, lambda protein phosphatase treatment converted the Ca2+/CaM-dependent form to the autonomous form of CaMKKβ/2, which was not affected by Ala mutation of Ser128, Ser132, and Ser136. The two activity forms of CaMKKβ/2 had equivalent Ca2+/CaM-binding ability. The findings demonstrate the presence of autonomous and Ca2+/CaM-dependent forms of CaMKKβ/2 independently in mouse tissues and cultured cells. The transition of these states of CaMKKβ/2 may be dynamically regulated by the phosphorylation/dephosphorylation of serine residues in the N-terminal regulatory domain.No potential conflict of interest relevant to this article was reported.Iron and Steel Institute of JapanActa Medica Okayama0915-155965132025Heat Transfer Enhancement by Forming Bridges among Reactive Particles in a Packed Bed Reactor of a Solid-gas Chemical Heat Storage System20972104ENKoichiNakasoFaculty of Environmental, Life Natural Science and Technology, Okayama UniversityKenjiShimadaGraduate School of Natural Science and Technology, Okayama UniversityYasushiMinoFaculty of Environmental, Life Natural Science and Technology, Okayama UniversityKuniakiGotohFaculty of Environmental, Life Natural Science and Technology, Okayama UniversityIn this study, the enhancement of the thermal output of solid-gas chemical heat storage systems was investigated. Bridges made of high-thermal conductivity materials were formed among reactive particles by drying a slurry which contained graphite powder as a thermal additive and dispersant in a packed-bed reactor. First, the effect of the volume ratio of the dispersant on effective thermal conductivity was investigated. The optimum volume ratio of dispersant to graphite powder was determined. Furthermore, repetitive bridge formation increased the effective thermal conductivity. Based on these results, we investigated the thermal response of the energy-discharge process. Consequently, the temperature distribution in the radial direction of the reactor decreased owing to the formation of bridges. In addition, the thermal energy generated by the adsorption of water vapor onto the adsorbent was effectively transferred to the reactor wall. The thermal output was estimated based on the experimental results. The thermal output was increased by the formation of bridges.No potential conflict of interest relevant to this article was reported.Ceramic Society of JapanActa Medica Okayama1348-653513412026Structure and acid resistance of fluoride-treated hydroxyapatite particles3137ENSatoshiHayakawaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazukiAndoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTomohikoYoshiokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTopical fluoride is used in clinical dentistry to prevent dental caries. The primary reaction product formed on the tooth surface after topical fluoride application is calcium fluoride (CaF2). In this study, we investigated the effect of deposited CaF2 on the acid resistance of fluoride-treated hydroxyapatite (HAp). Commercial HAp particles were treated with two types of fluoride-containing acetic acid-sodium acetate (Ac-AcNa) buffer solutions, with and without the addition of phosphate ions. X-ray diffraction (XRD) and nuclear magnetic resonance (NMR) analyses confirmed the formation of fluorine-substituted hydroxyapatite (F-HAp), along with a hydration layer containing calcium phosphate and CaF2. Phase composition analysis revealed that the presence of phosphate ions in the fluoride treatment solution reduced the amount of CaF2 deposited. Acid resistance evaluation and phase composition analysis indicated that the initial dissolution rate of the fluoride-treated HAp in Ac-AcNa buffer solutions decreased on surfaces densely covered with CaF2 particles.No potential conflict of interest relevant to this article was reported.Ceramic Society of JapanActa Medica Okayama1348-653513412026Preparation of brookite-type titanium dioxide particle layer on titanium surfaces via hydrothermal treatment and evaluation of in vitro apatite-forming ability2430ENSatoshiHayakawaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYushiNakamotoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySeiyaKojimaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNoriyukiNagaokaAdvanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental SchoolTakuyaKataokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTomohikoYoshiokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityIn this study, we prepared a brookite-type titanium dioxide particle layer on the surface of titanium substrates via hydrothermal treatment in aqueous urea solutions containing sodium chloride (NaCl) and examined its in vitro apatite-forming ability. Increasing the urea concentration suppressed the formation of anatase-type titanium dioxide on the titanium substrate, forming a particle layer composed of pure brookite-type titanium dioxide. The size and packing density of brookite-type titanium dioxide particles formed on the titanium substrate increased with the NaCl concentration in a 7.0 mol·dm−3 urea solution. When titanium substrates hydrothermally treated in aqueous solutions of 7.0 mol·dm−3 urea and 2.0 mol·dm−3 NaCl were soaked in a simulated body fluid for various periods up to 7 d, the substrate surface was densely covered with hemispherical apatite particles (5.3 µm in diameter) within 3 d, indicating that the brookite-type titanium dioxide particle layer had an excellent apatite-forming ability comparable to that of the anatase-type titanium dioxide particle layer.No potential conflict of interest relevant to this article was reported.Institute of Electrical and Electronics Engineers (IEEE)Acta Medica Okayama2169-3536132025Adaptive Topological Mapping With Free Area-Based Node Deletion for Autonomous Mobile RobotsENHarukaOzakiGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYuichiroTodaGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityNaokiMasuyamaGraduate School of Informatics, Osaka Metropolitan UniversityKaiFujiGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityTakayukiMatsunoGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityThis paper proposes an adaptive topological map building method, called Adaptive Resonance Theory-based Topological Clustering with Different Topologies (ATC-DT), for autonomous mobile robots using 3D point cloud data. ATC-DT framework integrates a novel node deletion mechanism that detects layout changes through free area detection. This allows the robot to update topological maps dynamically, removing outdated nodes caused by environmental changes. Experiments in real environments validate the ability of the method to perform global path planning, free area estimation, and adaptive navigation. The approach significantly improves navigation performance by improving map relevance and reducing redundancy of paths.No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama0010-94521942026Increasing visual uncertainty modulates multisensory decision-making5062ENXiangfuYangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityWeipingYangDepartment of Psychology, Faculty of Education, Hubei UniversityYinghuaYuGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYoshimichiEjimaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityJiajiaYangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityThe brain integrates and transforms information from multiple senses to make optimal decisions, a process that is critical for navigating complex environments with perceptual uncertainty. Despite a growing consensus that individuals adapt flexibly to uncertain sensory input, whether increasing visual uncertainty influences the decision process itself or other, non-decision sensory processes during multisensory decision-making are unclear. Here, an audiovisual categorization task was used to examine the responses of human participants (N = 30) to visual and audiovisual stimuli under low-, medium-, and high-uncertainty conditions. Modeling the behavioral data using a drift‒diffusion model indicated that increased visual uncertainty in the audiovisual context decreased the evidence accumulation rate but had no effect on non-decision processes. Electrophysiological recordings confirmed and expanded upon these results: increased visual uncertainty in the audiovisual context reduced the amplitude during the late decision-making stage (300–380 msec) but had no effect on the amplitude during the early sensory encoding stage (140–220 msec). More importantly, electroencephalography analyses revealed that audiovisual integration in the early sensory encoding stage occurred robustly across all visual uncertainty conditions, whereas audiovisual integration in the late stage occurred only under medium and high visual uncertainty conditions. This study demonstrated that increased visual uncertainty modulates the decision process itself rather than early sensory encoding during multisensory decision-making. Moreover, multisensory integration strategies dynamically adapt to increasing visual uncertainty by engaging different mechanisms to maintain effective decision-making.No potential conflict of interest relevant to this article was reported.Fuji Technology Press Ltd.Acta Medica Okayama1883-80493762025Directed Poisoning Attacks on FRIT in Adaptive Cruise Control13921399ENTaichiIkezakiFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityKenjiSawadaGraduate school of Mechanical Engineering, The University of OsakaOsamuKanekoGraduate School of Informatics and Engineering, The University of Electro-CommunicationsRecent advances in connected-vehicle technologies have enabled the large-scale collection of driving data, facilitating the deployment of data-driven control schemes. Although these methods offer advantages by eliminating the need for explicit modeling, they also introduce vulnerabilities due to their reliance on stored data. This study investigates a class of targeted data poisoning attacks on fictitious reference iterative tuning, a widely used data-driven controller tuning approach. We present a method that allows an adversary to influence closed-loop dynamics by manipulating the training data so that the resulting controller behavior matches a maliciously defined reference response. This strategy differs from conventional poisoning attacks, which aim only to the degrade control performance. Instead, it enables deliberate alteration of control characteristics such as overshoot and convergence time. The proposed attack is formulated as a constrained optimization problem under bounded tampering signals. Through a numerical study involving adaptive cruise control with stop functionality, we show that minor data modifications, indistinguishable from sensor noise, can cause significant degradation in control behavior. These findings highlight the need for robust security mechanisms in data-driven control implementation.No potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama2076-342515112025Sensory Modality-Dependent Interplay Between Updating and Inhibition Under Increased Working Memory Load: An ERP Study1178ENYuxiLuoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAoGuoDepartment of Psychology, Institute of Education, China West Normal UniversityJinglongWuFaculty of Biomedical Engineering, Shenzhen University of Advanced TechnologyJiajiaYangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityBackground/Objectives: Working memory (WM) performance relies on the coordination of updating and inhibition functions within the central executive system. However, their interaction under varying cognitive loads, particularly across sensory modalities, remains unclear. Methods: This study examined how sensory modality modulates flanker interference under increasing WM loads. Twenty-two participants performed a visual n-back task at three load levels (1-, 2-, and 3-back) while ignoring visual (within-modality) or auditory (cross-modality) flankers. Results: Behaviorally, increased WM load (2- and 3-back) led to reduced accuracy (AC) and prolonged reaction times (RTs) in both conditions. In addition, flanker interference was observed under the 2-back condition in both the visual within-modality (VM) and audiovisual cross-modality (AVM) tasks. However, performance impairment emerged at a lower load (2-back) in the VM condition, whereas in the AVM condition, it only emerged at the highest load (3-back). Significant performance impairment in the AVM condition occurred at higher WM loads, suggesting that greater WM load is required to trigger interference. Event-related potential (ERP) results showed that N200 amplitudes increased significantly for incongruent flankers under the highest WM load (3-back) in the visual within-modality condition, reflecting greater inhibitory demands. In the cross-modality condition, enhanced N200 was not observed across all loads and even reversed at low load (1-back). Moreover, the results also showed that P300 amplitude increased with load in the within-modality condition but decreased in the cross-modality condition. Conclusions: These results demonstrated that the interaction between updating and inhibition is shaped by both WM load and sensory modality, further supporting a sensory modality-specific resource allocation mechanism. The cross-modality configurations may enable more efficient distribution of cognitive resources under high load, reducing interference between concurrent executive demands.No potential conflict of interest relevant to this article was reported.Frontiers Media SAActa Medica Okayama1662-453X192025Effects of visual spatial frequency on audiovisual interaction: an event-related potential study1599114ENFengxiaWuSchool of Artificial Intelligence, Changchun University of Science and TechnologyYannaRenDepartment of Psychology, College of Humanities and Management, Guizhou University of Traditional Chinese MedicineTengfeiHaoSchool of Artificial Intelligence, Changchun University of Science and TechnologyJingjingYangSchool of Artificial Intelligence, Changchun University of Science and TechnologyQiongWuDepartment of Psychology, Suzhou University of Science and TechnologyJiajiaYangCognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama UniversityMengWangSchool of Artificial Intelligence, Changchun University of Science and TechnologySpatial frequency is a fundamental characteristic of visual signals that modulates the audiovisual integration behavior, but the neural mechanisms underlying spatial frequency are not well established. In the present study, the high temporal resolution of event-related potentials was used to investigate how visual spatial frequency modulates audiovisual integration. A visual orientation discrimination task was used, and the spatial frequency of visual stimuli was manipulated under three conditions. Results showed that the influence of visual spatial frequency on audiovisual integration is a dynamic process. The earliest audiovisual integration occurred over the left temporal-occipital regions in the early sensory stage (60–90 ms) for high spatial frequency conditions but was absent for low and middle spatial frequency conditions. In addition, audiovisual integration over fronto-central regions was delayed as spatial frequency increased (from 230–260 ms to 260–320 ms). The integration effect was also observed over parietal and occipital regions at 350–380 ms, and its strength gradually decreased at higher spatial frequencies. These discrepancies in the temporal and spatial distributions of audiovisual integration imply that the role of spatial frequency varies between early sensory and late cognitive stages. The findings of this study offer the first neural demonstration that spatial frequency modulates audiovisual integration, thus providing a basis for studying complex multisensory integration, especially in semantic and emotional domains.No potential conflict of interest relevant to this article was reported.AIP PublishingActa Medica Okayama0021-9606163222025Fourier-transform infrared spectroscopy of hydrogen fluoride dimers in solid parahydrogen224312ENYukiMiyamotoResearch Institute for Interdisciplinary Science, Okayama UniversityHirokiOoeGraduate School of Natural Science and Technology, Okayama UniversitySusumuKumaDepartment of Physics, Rikkyo UniversityWe investigate the Fourier-transform infrared spectra of hydrogen fluoride dimers in solid parahydrogen, the detailed analysis of which has remained unexplored. We propose a plausible analysis based on concentration dependence, light polarization, annealing, and time evolution. The absorption lines exhibited multiple peaks, with intensity ratios significantly altered by annealing and by time evolution at a constant temperature. The spectral patterns and isotopic effects suggest that the dimers do not rotate freely in solid parahydrogen, while multiple peaks arise from different stable structures, including single and double substitution sites. Unlike in the gas phase and helium droplets, no tunneling splitting was observed. The broad ν1 band suggests that some dimer structures may exhibit axial rotation. Spectral changes due to annealing likely result from site conversion, while observed IR-induced changes indicate preferential dissociation of dimers in double substitution sites. These findings still remain tentative, necessitating further experimental and theoretical studies.No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama1120-17971402025Improvements of lateral penumbra at various depth regions in proton pencil beam scanning with a multileaf collimator: Dose verifications and plan comparisons105684ENYukiTominagaDepartment of Radiotherapy, Medical Co. Hakuhokai, Osaka Proton Therapy ClinicYushiWakisakaDepartment of Radiotherapy, Medical Co. Hakuhokai, Osaka Proton Therapy ClinicTakahiroKatoDepartment of Radiological Sciences, School of Health Sciences, Fukushima Medical UniversityKeisukeYasuiSchool of Medical Sciences, Fujita Health UniversityRyoheiKatoDepartment of Radiation Physics and Technology, Southern Tohoku Proton Therapy CenterMasayaIchiharaMedical Physics Laboratory, Division of Health Science, Graduate School of Medicine, The University of OsakaMasashiTomidaDepartment of Proton Beam Technology Room, Narita Memorial Proton CenterMotoharuSasakiGraduate School of Biomedical Sciences, Tokushima UniversityMasatakaOitaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTeijiNishioMedical Physics Laboratory, Division of Health Science, Graduate School of Medicine, The University of OsakaPurpose: In scanned proton therapy, the current consensus is that the effective range of the collimator’s contribution to lateral penumbra improvement is up to approximately 150 mm depth. We characterized the penumbra variations for scanned proton beams with or without a new type of multileaf collimator (MLC) under various air gaps, depth, and with or without range shifter (RS).<br>
Methods: Eighty-six uniform dose plans were created (38 RS-negative and 48 RS-positive plans) for nine box targets of 60 × 60 × 54 mm3 at 0–280 mm depths in water. They were created with or without MLC, with 50–300 mm air gaps. The penumbra and average doses of MLC-positive and MLC-negative plans at the organs at risk (OAR) region of each box plan were compared. Besides, several plan doses were validated by measurements with penumbra (with an average of 80–20 % dose point widths for both side profiles) differences and 2D gamma analysis.<br>
Results: The MLC-positive plans reduced the penumbra and mean OAR doses by 1.0–5.1 mm and 3.3–13.5 %, respectively, compared to MLC-negative plans even at >150 mm depths. The penumbra differences in measurements were <±1.5 mm for all plans. The mean gamma scores at 2 %/2 mm were 97.9 ± 2.3 % and 97.4 ± 3.1 % for the MLC-negative and MLC-positive plans, respectively.<br>
Conclusions: The MLC-positive beams improved the penumbra and reduced the OAR dose in every depth region and air gap. We have shown that PBS with MLCs can be useful at more than 150 mm regions, depending on the machine.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama0960-741212012024OsHAK4 functions in retrieving sodium from the phloem at the reproductive stage of rice7690ENJingCheInstitute of Plant Science and Resources, Okayama UniversityNaokiYamajiInstitute of Plant Science and Resources, Okayama UniversityShao FeiWangKey Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesYueXiaKey Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesShun YingYangKey Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesYan HuaSuKey Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesRen FangShenKey Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesJian FengMaInstitute of Plant Science and Resources, Okayama UniversitySoil salinity significantly limits rice productivity, but it is poorly understood how excess sodium (Na+) is delivered to the grains at the reproductive stage. Here, we functionally characterized OsHAK4, a member of the clade IV HAK/KUP/KT transporter subfamily in rice. OsHAK4 was localized to the plasma membrane and exhibited influx transport activity for Na+, but not for K+. Analysis of organ- and growth stage-dependent expression patterns showed that very low expression levels of OsHAK4 were detected at the vegetative growth stage, but its high expression in uppermost node I, peduncle, and rachis was found at the reproductive stage. Immunostaining indicated OsHAK4 localization in the phloem region of node I, peduncle, and rachis. Knockout of OsHAK4 did not affect the growth and Na+ accumulation at the vegetative stage. However, at the reproductive stage, the hak4 mutants accumulated higher Na+ in the peduncle, rachis, husk, and brown rice compared to the wild-type rice. Element imaging revealed higher Na+ accumulation at the phloem region of the peduncle in the mutants. These results indicate that OsHAK4 plays a crucial role in retrieving Na+ from the phloem in the upper nodes, peduncle, and rachis, thereby preventing Na+ distribution to the grains at the reproductive stage of rice.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama2211-546315102025Osmotic pressure‐induced calcium response states17141722ENZidanGaoDepartment of Cardiovascular Physiology, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical SciencesKeijiNaruseFaculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Okayama JapanMasatoshiMorimatsuFaculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University Okayama JapanOsmotic pressure is essential for maintaining cellular homeostasis; however, the mechanisms by which cells sense and respond to acute osmotic stress remain incompletely understood. Here, we applied rapid osmotic pressure stimulation to cultured HEK293T cells and observed dynamic intracellular calcium responses. Acute hypotonic stimulation evoked calcium response patterns, whereas hypertonic and isotonic stress did not elicit similar effects. Mechanistically, these calcium signals originated from the endoplasmic reticulum via ryanodine receptor 2 and propagated to neighboring cells through Connexin 43-mediated gap junctions. These findings reveal a previously unrecognized role for calcium signaling in the acute cellular response to osmotic stress, providing new insights into the mechanisms of intercellular communication during osmotic adaptation.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama1613-68102025Atomic-Level Insights into Thermal Carbonization of Ethynyl-Containing Boron Compoundse13537ENKentaroOhkuraResearch Institute for Interdisciplinary Science, Okayama UniversitySatoshiHayakawaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNaokiTakahashiGraduate School of Environment Life Natural Science and Technology, Okayama UniversityKenYamazakiGraduate School of Environment Life Natural Science and Technology, Okayama UniversityJunKanoGraduate School of Environment Life Natural Science and Technology, Okayama UniversityYutaNishinaResearch Institute for Interdisciplinary Science, Okayama UniversityThis study reports the design, synthesis, and characterization of boron-doped carbon (BDC) derived from a triethynylborane-pyridine complex. Triethynylborane is stabilized by coordination with pyridine, facilitating its synthesis and handling in ambient conditions. The complex is subjected to thermal treatment at various temperatures to form BDC. Powder XRD and single-crystal XRD analyses reveal that BDC prepared at 200 °C retains an ordered structure, while higher temperatures induce alkyne structural changes without significant weight or surface area alterations. Coin cells are assembled using BDC as the anode, demonstrating unique Li-ion and Na-ion storage properties distinct from graphite. These results suggest that the BDC reflects the precursor's crystal structure, enabling novel electrochemical behavior. These findings offer insight into the development of advanced BDC materials for energy storage applications.No potential conflict of interest relevant to this article was reported.Oxford University Press (OUP)Acta Medica Okayama2730-6151512025Proliferation of a bloom-forming phytoplankton via uptake of polyphosphate-accumulating bacteria under phosphate-limiting conditionsycaf192ENSeiyaFukuyamaInstitute of Plant Science and Resources, Okayama UniversityFumikoUsamiInstitute of Plant Science and Resources, Okayama UniversityRyuichiHirotaGraduate School of Integrated Sciences for Life, Hiroshima UniversityAyanoSatohGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University ShizukaOharaGraduate School of Integrated Sciences for Life, Hiroshima UniversityKenKondoResearch Institute of Environment, Agriculture and Fisheries , Osaka PrefectureYukiGomibuchiDepartment of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of TechnologyTakuoYasunagaDepartment of Physics and Information Technology, Faculty of Computer Science and Systems Engineering, Kyushu Institute of TechnologyToshimitsuOndukaHatsukaichi Branch, Fisheries Technology Institute , Fisheries Research and Education AgencyAkioKurodaGraduate School of Integrated Sciences for Life, Hiroshima UniversityKazuhikoKoikeGraduate School of Integrated Sciences for Life, Hiroshima UniversityShokoUekiInstitute of Plant Science and Resources, Okayama UniversityHarmful algal blooms negatively impact the ecosystem and fisheries in affected areas. Eutrophication is a major factor contributing to bloom occurrence, and phosphorus is particularly important in limiting the growth of bloom-forming algae. Although algae efficiently utilize orthophosphate (Pi) as a phosphorous source over other molecular forms, Pi is often limited in the marine environment. While uptake and utilization of soluble inorganic and organic phosphorous by bloom-forming algae has been extensively studied, the details of geochemical and biological phosphorous cycling remain to be elucidated. Here, we report for the first time that the bloom-forming alga Heterosigma akashiwo can phagocytose bacteria and grow under phosphate-depleted conditions. The addition of Vibrio comitans to Pi-depleted H. akashiwo enabled the alga propagate to high cell densities, whereas other bacterial strains had only a minor effect. Importantly, V. comitans accumulates polyphosphate—a linear polymer of Pi—at high levels. The extent of algal proliferation induced by the addition of Vibrio species and polyphosphate-accumulating Escherichia coli correlated strongly with their polyphosphate content, indicating that bacterial polyphosphate served as an alternative PO43− source for H. akashiwo. The direct uptake of polyphosphate-accumulating bacteria through algal phagocytosis may represent a novel biological phosphorous-cycling pathway in marine ecosystems. The role of polyphosphate-accumulating marine bacteria as a hidden phosphorous source required for bloom formation warrants further investigation.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama1433-73982025A rare case of supratentorial ependymosarcoma harboring ZFTA::RELA fusionENYujiMatsumotoDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineYasukiSurugaDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineKaishiSatomiDepartment of Pathology, Faculty of Medicine, Kyorin UniversityYoheiInoueDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineYasuhikoHattoriDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineJojiIshidaDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineKazuhikoKurozumiDepartment of Neurosurgery, Hamamatsu University HospitalSumihitoNobusawaDepartment of Human Pathology, Gunma University School of MedicineJunkoHiratoDepartment of Pathology, Public Tomioka General HospitalTakehiroTanakaDepartment of Pathology, Okayama University HospitalHiroyukiYanaiDepartment of Pathology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineKanaWashioDepartment of Pediatrics, Okayama University HospitalKoichiIchimuraDepartment of Pathology, Faculty of Medicine, Kyorin UniversityTomotsuguIchikawaDepartment of Neurosurgery, Kagawa Prefectural Central HospitalYoshihiroOtaniDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineShotaTanakaDepartment of Neurological Surgery, Dentistry, and Pharmaceutical Sciences, Okayama University Graduate School of MedicineEpendymosarcoma is an exceedingly rare variant of ependymoma characterized by a mixture of ependymomatous and sarcomatous components. We report a case of supratentorial ependymosarcoma harboring a ZFTA::RELA fusion in a 10-year-old girl. Histologically, the tumor comprised an ependymomatous component resembling clear cell ependymoma and a sarcomatous component. ZFTA::RELA fusion was confirmed in both components. Genome-wide methylation profiling classified both components as supratentorial ependymoma, ZFTA fusion–positive by the German Cancer Research Center (DKFZ) CNS tumor classifier v12b8. However, their copy number alteration profiles were distinct. The ependymomatous component exhibited a gain of chromosome 1q and a loss of chromosomes 1p, 9, and 19q, while the sarcomatous component showed a loss of chromosome 14. These findings suggest that both components may have differentiated from a common precursor despite their distinct morphologies. The patient underwent gross total resection followed by adjuvant chemoradiotherapy and remains recurrence-free eight years post-treatment. Further investigation of additional cases is warranted to better understand the pathogenesis of this rare tumor.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Settlement Archaeology and Population Dynamics in KibiENWEST STEPHEN BRANDONGraduate School of Humanities and Social Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025日本語の会話における接続詞「で」についてENYANGLIUGraduate School of Humanities and Social Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025並列表現の史的展開と諸相－引用・疑問形式由来の「トカ」「トヤラ」を中心に－ENYUHENGJIGraduate School of Humanities and Social Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Study on neural mechanisms underlying multisensory spatiotemporal integration across visual, auditory, and tactile modalitiesENZIMOLIGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025テラヘルツ波ケミカル顕微鏡による低分子生体関連物質の検出と高感度プロトコルの開発ENCHANGJIANGLIUGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025大規模ネットワークにおける多重故障の高速迂回に関する研究ENHERINIAINA Joseph Hermann DorisGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025山口県育成小輪系ユリ切花の安定的周年供給体系の確立に関する研究ENYukoFUKUMITSUGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Strategic Orientation for Developing Municipal Solid Waste Management System towards Sound Material-Cycle Society - A Case Study of Central VietnamENLE DINH CUONGGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Improving sediment microbial fuel cells for water quality control in shrimp pond drainages in Central VietnamENNGUYEN TU UYENGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025３次元モデルの利用による港湾施工管理の効率化のための測深技術の開発ENHiromiNAKAHARAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025道路法面の3次元点群データに基づく変状抽出手法の確立と実用化に関する研究ENYujiHOSHINOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025サイドプランジ研削における研削温度の実験的検討とクーラント供給の最適化ENLingxiaoGaoGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Lightweight Deep Learning-Based Intrusion Detection System for Deployment on Raspberry PiENMUHAMMAD BISRI MUSTHAFAGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Integrated Kerberos-Blockchain Authentication Framework for Securing Vehicular Ad-Hoc Network ENMAYA RAHAYUGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A Blockchain-Based PGP Key Sharing Mechanism for Secure Email Communication ENMD. BIPLOB HOSSAINGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A Study of Indoor Navigation System Using Unity Game Engine and SmartphoneENEVIANITA DEWI FAJRIANTIGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A Study of Reference Paper Collection System Using Web Scraping and BERT ModelENINZALI NAINGGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Co-precipitating calcium phosphate as oral detoxification of cadmiumENAHMADBIKHARUDINGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231902025岡山大学教育学部教育実践力向上カリキュラムにおける初等家庭科内容基礎の実践 ― 2024 年度実践結果と教科内容構成力の継続的育成の検討 ―139148ENYokoSHINOHARAFaculty of Education, Okayama UniversityMiyukiHISANARI,Faculty of Education, Okayama UniversityChiharuMORIFaculty of Education, Okayama UniversityKyoungwonLEEFaculty of Education, Okayama University10.18926/bgeou/69617 岡山大学教育学部において教育実践力向上カリキュラムが始動し2 年目となる2024 年度「初等家庭科内容基礎」の実践について，学生のアンケート結果から新しいカリキュラムにおける授業評価と教科内容構成力育成の課題を検討した．授業の到達目標に係る学生の自己評価は高く，教科内容構成要素との関係から，教科内容構成力のプロセス①に係る基礎的な力は獲得できていた．授業に意欲的に取り組んだグループは授業全体の満足度が高かった．授業後は家庭科の教育内容を指導者の視点から認識できている学生が多く見られ，指導法で学ぶ家庭科の授業つくりに繋がる学修となっていることが分かった．No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231902025聴覚障害児の社会性と情緒の発達に関する現状と課題 ― これまでの教育実践と文献をもとに ―6371ENChitoseOKADAFaculty of Education, Okayama University10.18926/bgeou/69611 聴覚障害児を取り巻く状況は大きく変化してきているが，依然として諸課題の克服がなされているとは言い難い。その一つが「社会性と情緒の発達」である。「きこえない」がために，言語発達が遅れ，コミュニケーション障害をもたらし，さらに心理的問題等を生じるという深刻な二次的，三次的障害を引き起こす恐れがある。教師や保護者は，聴児が苦も無く行う「偶発学習」を意図的，計画的に促す必要がある。また，保護者は，早期からコミュニケーションの困難さを克服する努力を開始し，子どもの心に寄り添いながら，「言語的過保護」の状態を避けつつ様々な情報を丁寧に伝えていく必要がある。教師は，これらを常に意識しながら聴覚障害児と保護者双方の支援にあたり，多様な方法から実態に合うコミュニケーション方法を選択して指導に取り組み，社会性や情緒，さらに生きる力を育む必要がある。No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231902025安全を脅かすチームワークの崩壊とその関連要因の探索 ― 高信頼性チームの機能に着目して ―4761ENRyoMISAWAFaculty of Education, Okayama UniversityNaokoHASEGAWAFaculty of Human Sciences, Department of Psychology, Bunkyo University10.18926/bgeou/69610 本研究は，産業現場の安全を脅かす潜在的なリスクとしてチームワークの崩壊兆候に着目し，その特徴と高信頼性チーム（HRT）の機能，および組織環境要因との関連を実証的に検討した。安全活動への参加経験を持つ就業者357 名から得られたWeb 調査の回答を分析した。チームワークの崩壊兆候は「協調の失敗」と「修正の失敗」の2 因子に整理され，いずれもHRT 機能の発揮と負の関連を示した。また組織環境要因の安全風土やSafety Organizing は崩壊兆候の抑制に，学習のボトルネック認知はその増加に寄与していた。さらに，HRT 機能は個別よりも複合的に発揮されることで，チームの協働作業の円滑化・安定化に資する可能性が示唆された。No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama1471-22532512025Clinical predictors of extubation failure in postoperative critically ill patients: a post-hoc analysis of a multicenter prospective observational study127ENJunHattoriFaculty of Medicine, Osaka UniversityAikoTanakaDepartment of Anesthesiology and Intensive Care Medicine, Osaka University Graduate School of MedicineJunkoKosakaDepartment of Anesthesiology and Resuscitology, Okayama University HospitalOsamuHiraoDepartment of Anesthesiology, Osaka General Medical CenterNanaFurushimaDepartment of Anesthesiology and Intensive Care Medicine, Kobe University HospitalYuichiMakiDepartment of Anesthesiology, Toho University Ohashi Medical CenterDaijiroKabataCenter for Mathematical and Data Science, Kobe UniversityAkinoriUchiyamaDepartment of Anesthesiology and Intensive Care Medicine, Osaka University Graduate School of MedicineMoritokiEgiDepartment of Anesthesia, Kyoto University HospitalHiroshiMorimatsuDepartment of Anesthesiology and Resuscitology, Okayama University HospitalSatoshiMizobuchiDepartment of Anesthesiology and Intensive Care Medicine, Kobe University HospitalYoshifumiKotakeDepartment of Anesthesiology, Toho University Ohashi Medical CenterAyumiShintaniDepartment of Medical Statistics, Graduate School of Medicine, Osaka Metropolitan UniversityYukikoKoyamaDepartment of Anesthesiology and Intensive Care Medicine, Osaka University Graduate School of MedicineTakeshiYoshidaDepartment of Anesthesiology and Intensive Care Medicine, Osaka University Graduate School of MedicineYujiFujinoDepartment of Anesthesiology and Intensive Care Medicine, Osaka University Graduate School of MedicineBackground Postoperative patients constitute majority of critically ill patients, although factors predicting extubation failure in this group of patients remain unidentified. Aiming to propose clinical predictors of reintubation in postoperative patients, we conducted a post-hoc analysis of a multicenter prospective observational study.<br>
Methods This study included postoperative critically ill patients who underwent mechanical ventilation for > 24 h and were extubated after a successful 30-min spontaneous breathing trial. The primary outcome was reintubation within 48 h after extubation, and clinical predictors for reintubation were investigated using logistic regression analyses.<br>
Results Among the 355 included patients, 10.7% required reintubation. Multivariable logistic regression identified that the number of endotracheal suctioning episodes during the 24 h before extubation and underlying respiratory disease or pneumonia occurrence were significantly associated with reintubation (adjusted odds ratio [OR] 1.11, 95% confidence interval [CI] 1.05–1.18, p < 0.001; adjusted OR 2.58, 95%CI 1.30–5.13, p = 0.007). The probability of reintubation was increased significantly with the higher frequency of endotracheal suctioning, as indicated by restricted cubic splines. Subgroup analysis showed that these predictors were consistently associated with reintubation regardless of the use of noninvasive respiratory support after extubation.<br>
Conclusions Endotracheal suctioning frequency and respiratory complications were identified as independent predictors of reintubation. These readily obtainable predictors may aid in decision-making regarding the extubation of postoperative patients.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2041-17231612025A node-localized efflux transporter for loading iron to developing tissues in rice9916ENJingCheInstitute of Plant Science and Resources, Okayama UniversityShengHuangInstitute of Plant Science and Resources, Okayama UniversityYutingQuState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesYumaYoshiokaGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityChiyuriTomitaGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTakaakiMiyajiGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityZhenyangLiuState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesRenfangShenState Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of SciencesNaokiYamajiInstitute of Plant Science and Resources, Okayama UniversityJian FengMaInstitute of Plant Science and Resources, Okayama UniversityIron (Fe) is an essential micronutrient for plant growth and development. It plays crucial roles in various organs and tissues of plants, but the molecular mechanisms governing its distribution to the above-ground parts after root uptake remain unclear. In this study, we identify OsIET1 (Oryza sativa Iron Efflux Transporter 1), a rice gene highly expressed in the nodes. OsIET1 encodes a plasma membrane-localized protein, which shows efflux transport activity for ferrous iron. It is predominantly expressed in the xylem regions of diffuse vascular bundles, and its expression is upregulated under high Fe conditions. Disruption of OsIET1 impairs Fe allocation, reducing Fe transport to developing tissues (young leaves and grains), while increasing accumulation in nodes and older leaves. This misdistribution causes chlorosis in young leaves and decreases grain yield, especially under Fe-deficient conditions. Furthermore, we detect excessive Fe deposition around the xylem of diffuse vascular bundles in the nodes. Given the pivotal role of nodes in mineral distribution, our results indicate that OsIET1 mediates inter-vascular Fe transfer by facilitating Fe loading into the xylem of diffuse vascular bundles. This process ensures preferential Fe delivery to developing tissues, thereby promoting optimal plant growth and productivity.No potential conflict of interest relevant to this article was reported.Royal Society of Chemistry (RSC)Acta Medica Okayama1477-052023272025Fluorescence detection of DNA with a single-base mismatch by a Tm-independent peptide nucleic acid (PNA) twin probe65576563ENKokiIshiiDepartment of Applied Chemistry, Kindai UniversityHajimeShigetoHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)ShoheiYamamuraHealth and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)YoshitaneImaiDepartment of Applied Chemistry, Kindai UniversityTakashiOhtsukiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMizukiKitamatsuDepartment of Applied Chemistry, Kindai UniversityThere is a need to develop efficient methods for detecting target nucleic acids to enable the rapid diagnosis and early treatment of diseases. We previously demonstrated that a peptide nucleic acid (PNA) twin probe, consisting of two PNAs each containing a fluorescent dye, with pyrene at one end, detects target DNA sequence-specifically through pyrene excimer emission. In this study, to advance the development of this probe system, we further investigated the fluorescence properties of the PNA twin probe P1 and P2, and found that the excimer fluorescence was significantly reduced when a mismatched base in the DNA sequence was present at the site of P1 closest to the pyrene. In other words, this probe was found to detect single-base mismatches without taking into account the thermal stability of the PNA/DNA hybrid. The detection limit of this PNA twin probe for the single-base-mismatched DNA was 2.7 nM. In the future, this probe should lead to a method to detect point mutations in endogenous nucleic acids within cells.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama2328-95032025INF2-Related Charcot–Marie–Tooth Disease in a Japanese Cohort: Genetic and Clinical InsightsENChikashiYanoDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesMasahiroAndoDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesYujiroHiguchiDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesJun‐HuiYuanDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesAkikoYoshimuraDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesTakahiroHobaraDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesRisaNagatomoDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesFumikazuKojimaDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesYuHiramatsuDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesSatoshiNozumaDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesTomonoriNakamuraDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesYusukeSakiyamaDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesChikaMatsuokaDepartment of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesToruYamashitaDepartment of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesTakashiKimuraDepartment of Neurology, Hyogo Medical UniversityAyakoMiyazakiDepartment of Clinical Genetics, Hyogo Medical UniversityChinatsuKinjoDepartment of Clinical Genetics, Hyogo Medical UniversityKenjiYokochiDepartment of Pediatrics, Toyohashi Municipal HospitalNanamiYamanakaDepartment of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of MedicineNozomuMatsudaDepartment of Neurology, Fukushima Medical University School of MedicineTomokiSuichiDepartment of Neurology, Graduate School of Medicine, Chiba UniversityYoshiyukiHanaokaDepartment of Pediatrics, Kurashiki Central HospitalHarukaKojimaDepartment of Neurology, Tokyo Women's Medical UniversityKenichiTodoDepartment of Neurology, Tokyo Women's Medical UniversityHiroyukiIshiuraDepartment of Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesJunMitsuiDepartment of Precision Medicine Neurology, Graduate School of Medicine, The University of TokyoShojiTsujiDepartment of Neurology, The University of Tokyo HospitalHiroshiTakashimaDepartment of Neurology and Geriatrics, Kagoshima University Graduate School of Medical and Dental SciencesBackground: INF2 mutations cause focal segmental glomerulosclerosis (FSGS) and Charcot–Marie–Tooth disease (CMT). Accurate genetic diagnosis is critical, as INF2-related FSGS is typically resistant to immunotherapy yet rarely recurs after transplantation, and its associated neuropathy can mimic treatable immune-mediated disorders such as chronic inflammatory demyelinating polyradiculoneuropathy (CIDP).<br>
Methods: We performed a multicenter study investigating 3329 Japanese patients with inherited peripheral neuropathies/CMT who underwent gene panel sequencing or whole-exome analysis between 2007 and 2024. Clinical data, including electrophysiological assessments, were obtained from the patients' medical records.<br>
Results: We identified six pathogenic INF2 variants in eight patients, all of which were located within the diaphanous inhibitory domain. Structural modeling revealed clustering of variants near the diaphanous autoregulatory domain-binding pocket, which is critical for INF2 autoinhibition. Clinically, all cases were sporadic, with a median age at neurological onset of 9 years. All patients exhibited lower limb weakness, and 6/8 (75%) had sensory disturbances. All patients also developed kidney dysfunction, with 7/8 (88%) progressing to end-stage renal disease at a median age of 15 years. Furthermore, all patients showed demyelinating neuropathy, and 2/8 (25%) received immunotherapy due to suspected immune-mediated neuropathy.<br>
Conclusion: Although INF2 variants are a rare cause of CMT in Japan, they should be considered in pediatric patients with demyelinating neuropathy and early-onset proteinuria, even in the absence of a family history. Blood and urine tests assessing renal dysfunction can provide guidance for appropriate genetic testing.No potential conflict of interest relevant to this article was reported.Japan Institute of MetalsActa Medica Okayama0021-487689112025Ti-18Nb-xAl合金の構成相と材料特性に及ぼすAl添加量の影響337343ENYoshikazuMantaniDepartment of Materials Science and Engineering, National Institute of Technology (KOSEN), Suzuka CollegeYoshitoTakemotoFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityThe Ti-18mass%Nb alloy with a quenched α” martensitic structure exhibited a high damping capacity. However, there are issues such as lower strength than annealed α+β structure and decreasing damping capacity due to heating until 400 K. Therefore, in this study, to address these issues, we investigated the effect of Al addition on the constituent phases and material properties of Ti-18Nb-xAl alloys. The crystal structure was determined by examining the lattice constant and unit volume using X-ray diffraction, and optical microscopy was also performed. The material properties were investigated by Vickers hardness, Young’s modulus, internal friction, tensile tests, and DSC measurements. Vickers hardness and tensile strength increased with increasing Al content. This is thought to be due to the combined effects of the refinement of the microstructure and solid-solution strengthening due to Al addition. The Young’s modulus increased slightly from 0Al to 1Al, but increased significantly to 4Al. Internal friction was highest for 0Al and decreased for 4Al, whereas 7Al showed a higher value than 1Al. In the DSC heating curves, there was a decrease in the exothermic peak starting temperature and an increase in the phase-transformation heat with the addition of Al, except for 1Al. It was suggested that these changes in Ti-18Nb-xAl alloys were influenced by the structure of the quenched α” phase, texture, and pseudoelasticity or phase transformation by deformation.No potential conflict of interest relevant to this article was reported.岡山大学環境管理センターActa Medica Okayama0917-1533472025岡山大学環境管理センター報 第47号ENNo potential conflict of interest relevant to this article was reported.American Chemical Society (ACS)Acta Medica Okayama2694-24962025RNA Delivery Using a Graphene Oxide-Polyethylenimine Hybrid Inhibiting Myotube DifferentiationENKojiMatsuuraCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISISGiacomoReinaCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISISZhengfengGaoCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISISYutaNishinaResearch Institute for Interdisciplinary Science, Okayama UniversityAlbertoBiancoCNRS, Immunology, Immunopathology and Therapeutic Chemistry, UPR3572, University of Strasbourg, ISISGraphene oxide (GO) conjugated with short polyethylenimine (PEI) chains (GO-PEI) has been designed as a candidate nanocarrier for small interfering RNA (siRNA) delivery to mammalian cells based on the efficient interaction between the positively charged GO-based platform and the negatively charged siRNA. The function and efficiency of siRNA delivery using GO-PEI were compared to those using the positive control Lipofectamine RNAiMax by analyzing the differentiation to myotubes, and myogenin gene and protein expression in C2C12 cells. RNAiMax transfection induced cellularization and reduction of both myogenin gene and protein expression, suggesting that the differentiation of C2C12 cells was triggered by gene silencing. While GO-PEI also promoted cellularization, the myogenin gene expression remained comparable to scrambled controls, whereas the protein levels were higher than those observed with RNAiMax. Mechanistically, we attributed the reduced gene silencing efficiency of GO-PEI to a poor endosomal escape, despite strong siRNA complexation. This limitation was likely due to a low buffering capacity of GO-PEI, as a significant fraction of nitrogen atoms were already protonated, reducing the availability of free amines necessary for endosomal disruption. An appropriate chemical modification to enhance siRNA release from the endosomes is therefore essential for advancing the development of GO-based platforms as versatile and efficient nanocarriers in gene therapy applications.No potential conflict of interest relevant to this article was reported.Ceramic Society of JapanActa Medica Okayama1348-653513392025Preparation and structural characterization of nanoporous silica/magnesium(II)-whitlockite composite particles555561ENTakuyaKataokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityDaikiHirotaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityEijiFujiiIndustrial Technology Center of Okayama PrefectureTomohikoYoshiokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySatoshiHayakawaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityThe preparation of particles composed of nanoporous silica (NS) and Mg2+-whitlockite (Mg-WH) would provide valuable insights for designing particles for biomedical applications. In this study, NS and Mg-WH composite particles were successfully synthesized. The addition of chitosan during synthesis possibly promoted the crystallization of calcium phosphate phases in the composite particles. Pore size distribution analysis of the particles showed a maximum at 3.2 nm. Investigating the adsorption of methylene blue onto the particles in a phosphate buffer (pH 7.4) showed that the saturated adsorption amount of methylene blue on the particles was significantly higher than that on commercial hydroxyapatite. The composite particles provided important results for potential applications as drug carriers for bone regeneration and repair.No potential conflict of interest relevant to this article was reported.Ceramic Society of JapanActa Medica Okayama1348-653513312025Comparative study of the effects of fluoride treatment with cyclic variations in pH on the structures of stoichiometric, calcium-deficient, and carbonated hydroxyapatites1524ENSatoshiHayakawaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYuOkadaGraduate School of Natural Science and Technology, Okayama UniversityTomohikoYoshiokaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityThe primary objective of this study was to analyze the effects of fluoride treatment with cyclic variations in pH on the structure of stoichiometric hydroxyapatite (HAp), calcium-deficient HAp (CDHAp), and carbonated HAp (CHAp) powders. The structures of HAp, CDHAp, and CHAp before and after fluoride treatment were investigated using X-ray diffraction, Fourier-transform infrared, Raman, and nuclear magnetic resonance spectroscopic analyses. The fluoride treatment with cyclic variations in pH increased the calcium deficiency in HAp and CHAp but decreased in CDHAp. During fluoride treatment, fluoridated CDHAp or fluoridated calcium-deficient CHAp was formed on the surface of the HAp samples via dissolution and crystal growth, accompanied by the selective elution of component ions and partial substitution of OH− groups in the HAp hexagonal lattice with F− ions. No evidence of the formation of Ca(OH)2 and OH− groups outside the HAp crystal lattice was obtained. A new perspective on the formation of structured water at the surface termination of the OH columns (disordered region), with possible interactions with adsorbed water molecules or nonspecifically adsorbed F− ions was provided. The top surface of the fluoridated CDHAp consisted of an amorphous fluoride-rich hydrated layer, which included calcium phosphate and CaF2.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2731-0590492025Heart failure-specific cardiac fibroblasts contribute to cardiac dysfunction via the MYC–CXCL1–CXCR2 axis11351151ENJinKomuroDepartment of Cardiology, Keio University School of MedicineHisayukiHashimotoDepartment of Cardiology, Keio University School of MedicineToshiomiKatsukiDepartment of Cardiology, Keio University School of MedicineDaiKusumotoDepartment of Cardiology, Keio University School of MedicineManamiKatohDepartment of Frontier Cardiovascular Science, Graduate School of MedicineToshiyukiKoDepartment of Frontier Cardiovascular Science, Graduate School of MedicineMasamichiItoDepartment of Cardiovascular Medicine, Graduate School of Medicine, The University of TokyoMikakoKatagiriDepartment of Cardiovascular Medicine, Graduate School of Medicine, The University of TokyoMasayukiKubotaDepartment of Cardiovascular Medicine, Graduate School of Medicine, The University of TokyoShintaroYamadaDepartment of Cardiovascular Medicine, Graduate School of Medicine, The University of TokyoTakahiroNakamuraDepartment of Cardiology, Keio University School of MedicineYoheiAkibaDepartment of Cardiology, Keio University School of MedicineThukaaKoukaDepartment of Cardiology, Keio University School of MedicineKaorukoKomuroDepartment of Cardiology, Keio University School of MedicineMaiKimuraDepartment of Cardiology, Keio University School of MedicineShogoItoDepartment of Cardiology, Keio University School of MedicineSeitaroNomuraDepartment of Cardiovascular Medicine, Graduate School of Medicine, The University of TokyoIsseiKomuroDepartment of Frontier Cardiovascular Science, Graduate School of MedicineKeiichiFukudaDepartment of Cardiology, Keio University School of MedicineShinsukeYuasaDepartment of Cardiovascular Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesMasakiIedaDepartment of Cardiology, Keio University School of MedicineHeart failure (HF) is a growing global health issue. While most studies focus on cardiomyocytes, here we highlight the role of cardiac fibroblasts (CFs) in HF. Single-cell RNA sequencing of mouse hearts under pressure overload identified six CF subclusters, with one specific to the HF stage. This HF-specific CF population highly expresses the transcription factor Myc. Deleting Myc in CFs improves cardiac function without reducing fibrosis. MYC directly regulates the expression of the chemokine CXCL1, which is elevated in HF-specific CFs and downregulated in Myc-deficient CFs. The CXCL1 receptor, CXCR2, is expressed in cardiomyocytes, and blocking the CXCL1–CXCR2 axis mitigates HF. CXCL1 impairs contractility in neonatal rat and human iPSC-derived cardiomyocytes. Human CFs from failing hearts also express MYC and CXCL1, unlike those from controls. These findings reveal that HF-specific CFs contribute to HF via the MYC–CXCL1–CXCR2 pathway, offering a promising therapeutic target beyond cardiomyocytes.No potential conflict of interest relevant to this article was reported.岡山大学法学会Acta Medica Okayama0386-30507512025税理士の助言義務に関する一考察 ―相続時精算課税制度をめぐる問題を中心に―201ENH. TsujiNo potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama1422-006726172025Augmentation of the Benzyl Isothiocyanate-Induced Antiproliferation by NBDHEX in the HCT-116 Human Colorectal Cancer Cell Line8145ENRuitongSunGraduate School of Environmental and Life Science, Okayama UniversityAinaYanoGraduate School of Environmental and Life Science, Okayama UniversityAyanoSatohGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityShintaroMunemasaGraduate School of Environmental and Life Science, Okayama UniversityYoshiyukiMurataGraduate School of Environmental and Life Science, Okayama UniversityToshiyukiNakamuraGraduate School of Environmental and Life Science, Okayama UniversityYoshimasaNakamuraGraduate School of Environmental and Life Science, Okayama UniversityIncreased drug metabolism and elimination are prominent mechanisms mediating multidrug resistance (MDR) to not only chemotherapy drugs but also anti-cancer natural products, such as benzyl isothiocyanate (BITC). To evaluate the possibility of combined utilization of a certain compound to overcome this resistance, we focused on glutathione S-transferase (GST)-dependent metabolism of BITC. The pharmacological treatment of a pi-class GST-selective inhibitor, 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX), significantly increased BITC-induced toxicity in human colorectal cancer HCT-116 cells. However, NBDHEX unexpectedly increased the level of the BITC–glutathione (GSH) conjugate as well as BITC-modified proteins, suggesting that NBDHEX might increase BITC-modified protein accumulation by inhibiting BITC–GSH excretion instead of inhibiting GST. Furthermore, NBDHEX significantly potentiated BITC-induced apoptosis with the enhanced activation of apoptosis-related pathways, such as c-Jun N-terminal kinase and caspase-3 pathways. These results suggested that combination treatment with NBDHEX may be an effective way to overcome MDR with drug efflux and thus induce the biological activity of BITC at lower doses.No potential conflict of interest relevant to this article was reported.The Royal SocietyActa Medica Okayama1744-957X2172025Animal–chlorophyte photosymbioses: evolutionary origins and ecological diversityENIsabel Jiah-YihLiaoBiodiversity Research Center, Academia SinicaTosukeSakagamiBiodiversity Research Center, Academia SinicaThomas D.LewinBiodiversity Research Center, Academia SinicaXavierBaillyLaboratoire des Modèles Marins Multicellulaires, Station Biologique de RoscoffMayukoHamadaUshimado Marine Institute, Okayama UniversityYi-JyunLuoBiodiversity Research Center, Academia SinicaPhotosynthetic symbiosis occurs across diverse animal lineages, including Porifera, Cnidaria, Xenacoelomorpha and Mollusca. These associations between animal hosts and photosynthetic algae often involve the exchange of essential macronutrients, supporting adaptation to a wide range of aquatic environments. A small yet taxonomically widespread subset of animals host photosymbionts from the core chlorophytes, a phylogenetically expansive clade of green algae. These rare instances of ‘plant-like’ animals have arisen independently across distantly related lineages, resulting in striking ecological and physiological diversity. Although such associations provide valuable insights into the evolution of symbiosis and adaptation to novel ecological niches, animal–chlorophyte photosymbioses remain relatively understudied. Here, we present an overview of photosymbioses between animals and chlorophytes, highlighting their independent evolutionary origins, ecological diversity and emerging genomic resources. Focusing on Porifera, Cnidaria and Xenacoelomorpha, we review shared and lineage-specific adaptations underlying these associations. We also contrast them with dinoflagellate-based systems to demonstrate their distinct ecological and cellular features. Our work sets the stage for elucidating the molecular mechanisms underlying these associations, enhancing our understanding of how interspecies interactions drive adaptation to unique ecological niches through animal–chlorophyte symbiosis.No potential conflict of interest relevant to this article was reported.岡山医学会Acta Medica Okayama0030-155813722025第130回日本解剖学会／第102回日本生理学会／第98回日本薬理学会合同大会8081ENKeijiNaruseDepartment of Cardiovascular Physiology, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama1422-00672692025The Possibility of Plasma Membrane Transporters as Drug Targets in Oral Cancers4310ENChiharuSogawaDepartment of Food and Health Sciences, Faculty of Environmental Studies, Hiroshima Institute of TechnologyKatsumitsuShimadaDepartment of Clinical Phathophysiology, Matsumoto Dental UniversityKeisukeNakanoDepartment of Oral Pathology and Medicine, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityPlasma membrane transporters are increasingly recognized as potential drug targets for oral cancer, particularly oral squamous cell carcinoma (OSCC). These transporters play crucial roles in cancer cell metabolism, drug resistance, and the tumor microenvironment, making them attractive targets for therapeutic intervention. Among the two main families of plasma membrane transporters, ATP-binding cassette (ABC) transporters have long been known to be involved in drug efflux and contribute to chemoresistance in cancer cells. On the other hand, solute carriers (SLCs) are also a family of transporters that facilitate the transport of various substrates, including nutrients and drugs, and have recently been shown to contribute to cancer chemosensitivity, metabolism, and proliferation. SLC transporters have been identified as potential cancer biomarkers and therapeutic targets, and their expression profiles suggest that they could be utilized in precision oncology approaches. We summarize previous reports on the expression and role of ABC and SLC transporters in oral cancer and discuss their potential as therapeutic targets.No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231892025論争問題学習における「当事者性」を保障する授業設計 ―地理歴史科「歴史総合」小単元「紛争鉱物から考える私たちの社会」の開発を事例として―87100ENToshinoriKUWABARAFaculty of Education，Okayama UniversityMichikoKAMITADepartment of Primary Education, Faculty of Education, Okayama University of Science10.18926/bgeou/69240 社会問題についての熟議や意思決定，合意形成には当事者性が必要である。当事者性がなければ，皆が納得する答え，すなわち公共善を構築することはできない。しかし，当事者性を強調することがかえって生徒の「思考停止」を導くという批判もあり，当事者性を保障する以前に、当事者性そのものの概念を再考する必要がある。
そこで，本研究では，「思考停止」に至らない当事者性とはどのようなものか，先行研究をもとに再定義を行う。その定義を踏まえて、学習者に「当事者性」を保障する授業構成のあり方を考察し，地理歴史科「歴史総合」の小単元「紛争鉱物から考えるグローバル社会」の開発を行う。No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231892025二分脊椎症の病態理解に基づく教育現場での支援6774ENIoriOHMORI (KAWASAKI)Faculty of Education，Okayama UniversityMitsukoYAMANAKAOkayama prefectural Okayama east special needs school10.18926/bgeou/69238 二分脊椎症のある子どもは、症状の個人差が大きく、肢体不自由特別支援学校だけでなく普通学級にも多く在籍している。本研究の目的は，二分脊椎症の病態に基づき、教育現場での支援の在り方について、先行研究の知見をまとめ、教育上の課題をあきらかにすることである。<br>
二分脊椎症には、下肢の運動障害と感覚障害、排泄障害に加え、水頭症をはじめとした様々な合併症があることから、運動面、生活面、認知面において適切な支援を要する。さらに、思春期以降になると、脊髄係留による疼痛の出現や、歩行能力の低下に注意する必要がある。神経因性膀胱による排尿障害を合併する場合は、清潔間欠導尿を行う。学校生活を楽しいと肯定的に捉えていた子どもたちのほとんどは、清潔間欠導尿を介助無しあるいは一部の介助のみで排尿することができていた。このことから、担任は、身体的、認知的な支援と配慮に加え、多職種と密な連携を取りながら清潔間欠自己導尿を目指した自立活動の指導を進めることと、良好な友人関係を築くための支援を行うことが重要と思われた。No potential conflict of interest relevant to this article was reported.岡山大学大学院教育学研究科Acta Medica Okayama1883-24231892025バーチャルチーム研究のパースペクティブ ― DX 時代のチームマネジメントが直面するパラドックス ―3756ENRyoMISAWAFaculty of Education，Okayama UniversityMakotoFUJIMURAFaculty of Human Relations, Fukuoka Jo Gakuin University10.18926/bgeou/69236 情報通信技術の発展や新型コロナウイルス感染症の感染拡大を契機として，組織におけるバーチャルチーム（virtual team; VT）の活用が広がっている。本稿では，VT に関する理論的・実証的知見を整理し，DX 時代におけるチームマネジメントの課題と今後の研究の方向性を検討した。まず，VT の概念定義とその中核をなすバーチャル性の構成要素（地理的分散，テクノロジーの使用など）を確認し，VT の効果性を説明する主要な理論モデルをレビューした。また，VT の構造に内在する「バーチャル性のパラドックス」に着目し，「柔軟性と構造化」や「独立と相互依存」など，相反する要求が同時に存在する持続的な緊張状態と，それに対応するための両立の発想に基づくマネジメントの重要性を考察した。No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama0924-42473902025Extension-type flexible pneumatic actuator with a large extension force using a cross-link mechanism based on pantographs116594ENSoShimookaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityKazumaTadachiMechanical and Systems Engineering Program, School of Engineering, Okayama UniversityTetsushiKamegawaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityIn this study, we propose an extension-type flexible pneumatic actuator (EFPA) with a high extension force and no buckling. In a previous study, soft actuators that extended in the axial direction by applying a supply pressure were unable to generate the extension’s pushing force because the actuators buckled owing to their high flexibility. To generate a pushing force, the circumferential stiffness of an extension-type flexible soft actuator must be reinforced. Therefore, a cross-linked EFPA (CL-EFPA) was developed, inspired by a pantograph that restrains the EFPA three-dimensionally using the proposed link mechanism. The proposed CL-EFPA consists of three EFPAs and a cross-linking mechanism for integrating each EFPA circumference. The pushing force of the CL-EFPA is approximately 3.0 times compared with that generated by the previous EFPA with plates to restrain its plane. To perform various bending motions, attitude control was performed using an analytical model and a system that included valves, sensors, and controllers.No potential conflict of interest relevant to this article was reported.Fuji Technology Press Ltd.Acta Medica Okayama1883-80493712025Fan-Shaped Pneumatic Soft Actuator that Can Operate Bending Motion for Ankle-Joint Rehabilitation Device4353ENSoShimookaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityHirosatoYokoyaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasanoriHamadaDepartment of Rehabilitation Medicine, Okayama University HospitalShunShiomiDepartment of Rehabilitation Medicine, Okayama University HospitalTakenoriUeharaDepartment of Orthopaedic Surgery, NHO Okayama Medical CenterTakahiroHirayamaDepartment of Emergency, Critical Care and Disaster Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTetsushiKamegawaFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityNowadays, owing to declining birthrates and an aging population, patients and the elderly requiring rehabilitation are not getting enough physical activity. In addressing this issue, devices for rehabilitating them have been researched and developed. However, rehabilitation devices are almost exclusively used for patients who can get up, rather than those who are bedridden. In this study, we aim to develop a rehabilitation device that can provide passive exercise for bedridden patients. The ankle joint was selected as the target joint because the patients who have undergone surgery for cerebrovascular disease remain bedridden, and early recovery in the acute stage is highly desirable. We proposed and tested a fan-shaped pneumatic soft actuator (FPSA) that can expand and bend stably at angles when supply pressure is applied as an actuator for a rehabilitation device to encourage patient exercise. However, the previous FPSA’s movement deviates from the arch of the foot owing to increased supply pressure. In the ideal case, FPSA should push the arch of the foot in an arc motion. This study proposes and tests the FPSA that can operate a bending motion to provide passive exercise to the ankle joint using tensile springs and a winding mechanism powered by a servo motor. The proposed FPSA has a significant advantage of exhibiting no hysteresis in its pressure-displacement characteristics. The configuration and static analytical model of the improved FPSA are described.No potential conflict of interest relevant to this article was reported.American Chemical Society (ACS)Acta Medica Okayama2691-3704522025Mechanistic Insights Into Oxidative Response of Heat Shock Factor 1 Condensates606617ENSoichiroKawagoeInstitute of Advanced Medical Sciences, Tokushima UniversityMotonoriMatsusakiInstitute of Advanced Medical Sciences, Tokushima UniversityTakuyaMabuchiFrontier Research Institute for Interdisciplinary Sciences, Tohoku UniversityYutoOgasawaraDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazunoriWatanabeDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKoichiroIshimoriDepartment of Chemistry, Faculty of Science, Hokkaido UniversityTomohideSaioInstitute of Advanced Medical Sciences, Tokushima UniversityHeat shock factor 1 (Hsf1), a hub protein in the stress response and cell fate decisions, senses the strength, type, and duration of stress to balance cell survival and death through an unknown mechanism. Recently, changes in the physical property of Hsf1 condensates due to persistent stress have been suggested to trigger apoptosis, highlighting the importance of biological phase separation and transition in cell fate decisions. In this study, the mechanism underlying Hsf1 droplet formation and oxidative response was investigated through 3D refractive index imaging of the internal architecture, corroborated by molecular dynamics simulations and biophysical/biochemical experiments. We found that, in response to oxidative conditions, Hsf1 formed liquid condensates that suppressed its internal mobility. Furthermore, these conditions triggered the hyper-oligomerization of Hsf1, mediated by disulfide bonds and secondary structure stabilization, leading to the formation of dense core particles in the Hsf1 droplet. Collectively, these data demonstrate how the physical property of Hsf1 condensates undergoes an oxidative transition by sensing redox conditions to potentially drive cell fate decisions.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama0094-24055282025Commissioning of respiratory‐gated 4D dynamic dose calculations for various gating widths without spot timestamp in proton pencil beam scanninge18026ENYukiTominagaDepartment of Radiotherapy, Medical Co. Hakuhokai, Osaka Proton Therapy ClinicYushiWakisakaDepartment of Radiotherapy, Medical Co. Hakuhokai, Osaka Proton Therapy ClinicTakahiroKatoDepartment of Radiological Sciences, School of Health Sciences, Fukushima Medical UniversityMasayaIchiharaMedical Physics Laboratory, Division of Health Science, Graduate School of Medicine, The University of OsakaKeisukeYasuiSchool of Medical Sciences, Fujita Health UniversityMotoharuSasakiGraduate School of Biomedical Sciences, Tokushima UniversityMasatakaOitaFaculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityTeijiNishioMedical Physics Laboratory, Division of Health Science, Graduate School of Medicine, The University of OsakaBackground: Proton pencil beam scanning (PBS) is susceptible to dose degradation because of interplay effects on moving targets. For cases of unacceptable motion, respiratory-gated (RG) irradiation is an effective alternative to free breathing (FB) irradiation. However, the introduction of RG irradiation with larger gate widths (GW) is hindered by interplay effects, which are analogous to those observed with FB irradiation. Accurate estimation of interplay effects can be performed by recording spot timestamps. However, our machine lacks this feature, making it imperative to find an alternative approach. Thus, we developed an RG 4-dimensional dynamic dose (RG-4DDD) system without spot timestamps.<br>
Purpose: This study aimed to investigate the accuracy of calculated doses from the RG-4DDD system for PBS plans with varying breathing curves, amplitudes, and periods for 10%–50% GW.<br>
Methods: RG-4DDDs were reconstructed using in-house developed software that assigned timestamps to individual spots, integrated start times for spills with breathing curves, and utilized deformable registrations for dose accumulation. Three cubic verification plans were created using a heterogeneous phantom. Additionally, typical liver and lung cases were employed for patient plan validation. Single- and multi-field-optimized (SFO and IMPT) plans (ten beams in total) were created for the liver and lung cases in a homogeneous phantom. Lateral profile measurements were obtained under both motion and no-motion conditions using a 2D ionization chamber array (2D-array) and EBT3 Gafchromic films on the CIRS dynamic platform. Breathing curves from the cubic plans were used to assess nine patterns of sine curves, with amplitudes of 5.0–10.0 mm (10.0–20.0 mm target motions) and periods of 3–6 sec. Patient field verifications were conducted using a representative patient curve with an average amplitude of 6.4 mm and period of 3.2 sec. Additional simulations were performed assuming a ± 10% change in assigned timestamps for the dose rate (DR), spot spill (0.08-s), and gate time delay (0.1-s) to evaluate the effect of parameter selection on our 4DDD models. The 4DDDs were compared with measured values using the 2D gamma index and absolute doses over that required for dosing 95% of the target.<br>
Results: The 2D-array measurements showed that average gamma scores for the reference (no motion) and 4DDD plans for all GWs were at least 99.9 ± 0.2% and 98.2 ± 2.4% at 3%/3 mm, respectively. The gamma scores of the 4DDDs in film measurements exceeded 95.4% and 92.9% at 2%/2 mm for the cubic and patient plans, respectively. The 4DDD calculations were acceptable under DR changes of ±10% and both spill and gate time delays of ±0.18 sec. For the 4DDD plan using all GWs for all measurement points, the absolute point differences for all validation plans were within ±5.0% for 99.1% of the points.<br>
Conclusions: The RG-4DDD calculations (less than 50% GW) of the heterogeneous and actual patient plans showed good agreement with measurements for various breathing curves in the amplitudes and periods described above. The proposed system allows us to evaluate actual RG irradiation without requiring the ability to record spot timestamps.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama1071-269060102024Enhanced design of pCMViR-TSC plasmid vector for sustainably high cargo gene expression in mammalian cells12151227ENMasakiyoSakaguchiDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineRieKinoshitaDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineNahokoTomonobuDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineYoshihikoSakaguchiDepartment of Microbiology, Tokushima Bunri UniversityJunichiroFutamiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAkiraYamauchiDepartment of Biochemistry, Kawasaki Medical SchoolHitoshiMurataDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineKen-ichiYamamotoDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineTettaTakahashiDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineYumaGoharaDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineToshikiOchiDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineFanJiangDepartment of Cell Biology, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineNi Luh Gede YoniKomalasariFaculty of Medicine, Udayana UniversityYouyiChenDepartment of Breast Surgery, The First Affiliated Hospital, Zhejiang University School of MedicineI Made WinarsaRumaFaculty of Medicine, Udayana UniversityI WayanSumardikaFaculty of Medicine, Udayana UniversityJinZhouMedical Oncology Department of Gastrointestinal Tumors, Liaoning Cancer Hospital & Institute, Cancer Hospital of the Dalian University of TechnologyTomokoHonjoDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityFutoshiKuribayashiDepartment of Biochemistry, Kawasaki Medical SchoolKazumiSagayamaOrganization for Research and Innovation Strategy, Okayama UniversityShinichiToyookaDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Dentistry and Pharmaceutical Sciences, Okayama University Graduate School of MedicineEisakuKondoDivision of Tumor Pathology, Near InfraRed Photo-Immuno-Therapy Research Institute, Kansai Medical UniversityYusukeInoueFaculty of Science and Technology, Division of Molecular Science, Gunma UniversityThe first-generation pCMViR-TSC, implemented through the promoter sandwich rule, yields 10- to 100-fold higher gene expression than the standard plasmid used with the CMV (cytomegalovirus) or CAG promoter. However, the vector’s shortcomings limit its utility to transient expression only, as it is not suitable for establishing stable transformants in mammalian cells. To overcome this weakness, we here introduce the improved plasmid vector pSAKA-4B, derived from pCMViR-TSC as a second-generation chromosome-insertable vector. This vector facilitates the linear entry of the expression unit into the TTAA site of DNA universally with transposase assistance. The vector is helpful for the indefinite expression of our target gene. The new vector system is proven here to be efficient in establishing stable transformants with a high likelihood of positive clones that exhibit significantly elevated expression levels of the delivered foreign gene. This system, alongside the first-generation vector, is therefore instrumental for diverse basic research endeavors concerning genes, proteins, cells, and animals, and potentially for clinical applications such as gene therapy.No potential conflict of interest relevant to this article was reported.IOS PressActa Medica Okayama0926-96302025Assessing the Proportion of Clinical Trial Eligibility Criteria Expressible with Standard EHR Data Elements18921893ENRisaOkazakiDepartment of Pharmacy, Okayama University HospitalKunihisaKamikawaCenter for Innovative Clinical Medicine, Okayama University HospitalHidekiUnoCenter for Innovative Clinical Medicine, Okayama University HospitalHirotoOkudaDivision of Clinical Research of New Drugs and Therapeutics, Center for Innovative Clinical Medicine, Okayama University HospitalShihokoNambaDivision of Clinical Research of New Drugs and Therapeutics, Center for Innovative Clinical Medicine, Okayama University HospitalMitsunobuKanoGraduate School of Interdisciplinary Science and Technology in Health Systems, Okayama UniversityMizukiMoritaGraduate School of Interdisciplinary Science and Technology in Health Systems, Okayama UniversityPatient recruitment for clinical trials often requires substantial human effort and experiences delays, leading to increased drug development costs. Leveraging electronic health records (EHRs) may improve the accuracy of estimates of potentially recruitable patients. We evaluated the feasibility of using EHRs by analyzing the proportion of computable eligibility criteria.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2045-23221512025Autoantibody spark response predicts treatment outcome in patients receiving chemoradiation followed by durvalumab therapy27502ENTakeruMoriGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMioKitagawaDepartment of Radiology, Sapporo Medical University School of MedicineTomokazuHasegawaDepartment of Radiology, Sapporo Medical University School of MedicineMasanoriSomeyaDepartment of Radiology, Sapporo Medical University School of MedicineTakaakiTsuchiyaDepartment of Radiology, Sapporo Medical University School of MedicineToshioGochoDepartment of Radiology, Sapporo Medical University School of MedicineTomokoHonjoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMireiDateGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMarikoMoriiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAiMiyamotoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityJunichiroFutamiGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityThe PACIFIC regimen, comprising chemoradiotherapy (CRT) followed by maintenance with the immune checkpoint inhibitor (ICI) durvalumab, has become the standard of care for patients with unresectable non-small cell lung cancer (NSCLC). Although ICI is used to prevent recurrence by targeting residual microtumors, biomarkers capable of monitoring immune activity during this phase remain lacking. Here, we evaluated whether temporal changes in serum autoantibody levels can predict treatment efficacy. This retrospective study included 20 patients with unresectable stage II or III NSCLC who received the PACIFIC regimen. Serum autoantibodies against 130 antigens were quantified before CRT, after CRT, and two weeks after the first ICI dose. The primary outcome was progression-free survival (PFS), and its association with autoantibody dynamics was examined. We observed an immediate and strong autoantibody response (spark response [SR]) after ICI initiation in patients with favorable treatment outcomes. Patients with SR and programmed death ligand 1 (PD-L1) expression ≥ 50% showed better PFS (two-year PFS; 72.9% vs. 18.2%, p = 0.0021). These findings suggest that serial monitoring of serum autoantibodies can provide a noninvasive approach to assess immune activity and predict treatment outcomes in patients receiving CRT or ICI therapy.No potential conflict of interest relevant to this article was reported.Proceedings of the National Academy of SciencesActa Medica Okayama0027-8424122322025Structural insights into a citrate transporter that mediates aluminum tolerance in barleye2501933122ENTranNguyen ThaoDegree Program in Interdisciplinary Sciences, Graduate School of Environmental, Life, Natural Science, and Technology, Okayama UniversityNamikiMitani-UenoResearch Core for Plant Stress Science, Institute of Plant Science and Resources, Okayama UniversityRyoUranoDivision of Superconducting and Functional Materials, Research Institute for Interdisciplinary Science, Okayama UniversityYasunoriSaitohDegree Program in Interdisciplinary Sciences, Graduate School of Environmental, Life, Natural Science, and Technology, Okayama UniversityPeitongWangResearch Core for Plant Stress Science, Institute of Plant Science and Resources, Okayama UniversityNaokiYamajiResearch Core for Plant Stress Science, Institute of Plant Science and Resources, Okayama UniversityJian-RenShenDegree Program in Interdisciplinary Sciences, Graduate School of Environmental, Life, Natural Science, and Technology, Okayama UniversityWataruShinodaDegree Program in Interdisciplinary Sciences, Graduate School of Environmental, Life, Natural Science, and Technology, Okayama UniversityJian FengMaResearch Core for Plant Stress Science, Institute of Plant Science and Resources, Okayama UniversityMichihiroSugaDegree Program in Interdisciplinary Sciences, Graduate School of Environmental, Life, Natural Science, and Technology, Okayama UniversityHvAACT1 is a major aluminum (Al)-tolerance gene in barley, encoding a citrate transporter that belongs to the multidrug and toxic compound extrusion (MATE) family. This transporter facilitates citrate secretion from the roots, thereby detoxifying external Al ions—a major constraint of crop production on acidic soils. In this study, we present the outward-facing crystal structure of HvAACT1, providing insights into a citrate transport mechanism. The putative citrate binding site consists of three basic residues—K126 in transmembrane helix 2 (TM2), R358 in TM7, and R535 in TM12—creating substantial positive charges in the C-lobe cavity. Proton coupling for substrate transport may involve two pairs of aspartate residues in the N-lobe cavity, one of which corresponds to the essential Asp pair found in prokaryotic H+-coupled MATE transporters belonging to the DinF subfamily. Structural coupling between proton uptake in the N-lobe and citrate extrusion in the C-lobe can be enabled by an extensive, unique hydrogen-bonding network at the extracellular half of the N-lobe. Mutation-based functional analysis, structural comparisons, molecular dynamics simulation, and phylogenic analysis suggest an evolutionary link between citrate MATE transporters and the DinF MATE subfamily. Our findings provide a solid structural basis for citrate transport by HvAACT1 in barley and contribute to a broader understanding of citrate transporter structures in other plant species.No potential conflict of interest relevant to this article was reported.Oxford University Press (OUP)Acta Medica Okayama0022-09572025Dual roles of suberin deposition at the endodermal Casparian strip in manganese uptake of rice111ENToshikiFujiiInstitute of Plant Science and Resources, Okayama UniversityNaokiYamajiInstitute of Plant Science and Resources, Okayama UniversityJian FengMaInstitute of Plant Science and Resources, Okayama UniversityRice roots are characterized by having two Casparian strips (CSs) at the exodermis and endodermis, where transporters for mineral nutrients are expressed. However, the exact role of the CS in expression of the transporters and subsequent nutrient uptake is poorly understood. Here, we first investigated the role of the CS in manganese (Mn) uptake by using a rice mutant (oscasp1) defective in formation of the endodermal CS. Knockout of OsCASP1 resulted in decreased Mn uptake under limited Mn conditions, but increased Mn uptake at high Mn concentration. Immunostaining revealed that knockout of OsCASP1 did not affect the cell specificity of localization of two transporters (OsNramp5 and OsMTP9) required for Mn uptake, but decreased the protein abundance of these transporters at the endodermis regardless of Mn concentrations tested. Furthermore, we found that overaccumulation of suberin at the endodermis of the mutants suppressed the expression of two transporters; the expression of the two transporters was only observed in the endodermal cells without suberin deposition, but not in the cells with suberin deposition. Taken together, our results indicate that there are two roles for the CS in Mn uptake; maintaining normal expression of the transporters at limited Mn concentration and preventing Mn diffusion to the stele at high Mn concentration.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama0952-34803892025Ultrahigh‐Field MR‐Compatible Mechanical Tactile Stimulator for Investigating Somatosensory Processing in Small‐Bodied Animalse70105ENChenyuWangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityHirohikoImaiInnovation Research Center for Quantum Medicine, Gifu University School of MedicineMasakiFukunagaSection of Brain Function Information, National Institute for Physiological SciencesHirokiYamamotoGraduate School of Human and Environmental Studies, Kyoto UniversityYinghuaYuGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazuhikoSekiDepartment of Neurophysiology, National Center of Neurology and PsychiatryTakashiHanakawaDepartment of Integrated Neuroanatomy and Neuroimaging, Kyoto University Graduate School of MedicineTatsuyaUmedaDepartment of Integrated Neuroanatomy and Neuroimaging, Kyoto University Graduate School of MedicineJiajiaYangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityCommon marmosets (Callithrix jacchus), small-bodied New World primates that share similar sensory processing pathways with human beings, have gained great interests. Their small body size allows imaging of brain activity with high spatial resolution and on a whole-brain scale using ultrahigh-field (UHF) magnetic resonance imaging (MRI) scanners. However, the strong magnetic field and the small size of the hand and forearm pose challenges in delivering tactile stimulation during fMRI experiments. In the present study, we developed an MR-compatible tactile dual-point stimulator to provide high-precision mechanical stimulation for exploring somatosensory processing in small-bodied animals. The study population consisted of a water phantom and three male common marmosets. Cerebral blood volume (CBV) weighted fMRI data were obtained with a gradient echo (GE), echo-planar imaging (EPI) sequence at 7T scanner. The output performance of the device was tested by a pressure sensor. The MR compatibility of the device was verified by measuring the temporal signal-to-noise ratio (tSNR) of a water phantom. To test the effectiveness of tactile stimulation, we conducted block designed tactile stimulation experiments on marmosets. A one-way repeated measures ANOVA was conducted for comparing the tSNR results. We performed one-sample t-tests to investigate the negative response of the forearm and hand stimulation with a threshold of t > 1.96 (p < 0.05). Performance tests revealed that mechanical stimulation (averaged force: 31.69 g) was applied with a delay of 12 ms. Phantom experiments confirmed that there was no significant difference in the tSNR among three (10 Hz, 1 Hz, and no-stimulus) conditions (F (2, 798) = 0.71, p = 0.49). The CBV activity results showed that the stimulator successfully elicited hand and forearm somatosensory activations in primary somatosensory areas. These results indicated that the device is well suited for small-bodied animal somatosensory studies.No potential conflict of interest relevant to this article was reported.JMIR Publications Inc.Acta Medica Okayama2561-326X92025Usefulness of Interventions Using a Smartphone Cognitive Behavior Therapy Application for Children With Mental Health Disorders: Prospective, Single-Arm, Uncontrolled Clinical Triale60943ENShinichiroNagamitsuDepartment of Pediatrics, Faculty of Medicine, Fukuoka UniversityAyumiOkadaDepartment of Pediatrics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesRyoichiSakutaChild Development and Psychosomatic Medicine Center, Dokkyo Medical University Saitama Medical CenterRyutaIshiiDepartment of Pediatrics & Child Health, Kurume University, School of MedicineKenshiKoyanagiNagasaki Prefectural Center of Medicine and Welfare for ChildrenChizuHabukawaDepartment of Pediatric Allergy, Minami Wakayama Medical CenterTakashiKatayamaL2B IncMasayaItoNational Center for Cognitive Behavior Therapy and Research, National Center of Neurology and PsychiatryAyakoKanieNational Center for Cognitive Behavior Therapy and Research, National Center of Neurology and PsychiatryRyokoOtaniChild Development and Psychosomatic Medicine Center, Dokkyo Medical University Saitama Medical CenterTakeshiInoueChild Development and Psychosomatic Medicine Center, Dokkyo Medical University Saitama Medical CenterTasukuKitajimaChild Development and Psychosomatic Medicine Center, Dokkyo Medical University Saitama Medical CenterNaokiMatsubaraChild Development and Psychosomatic Medicine Center, Dokkyo Medical University Saitama Medical CenterChieTanakaDepartment of Pediatrics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesChikakoFujiiDepartment of Pediatrics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesYoshieShigeyasuDepartment of Pediatrics, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesMichikoMatsuokaDepartment of Neuropsychiatry, Kurume University School of MedicineTatsuyukiKakumaBiostatistics Center, Kurume UniversityMasaruHorikoshiNational Center for Cognitive Behavior Therapy and Research, National Center of Neurology and PsychiatryBackground: The prevalence of mental health disorders among children in Japan has increased rapidly, and these children often show depressive symptoms and reduced quality of life (QOL). We previously developed a smartphone-based self-monitoring app to deliver cognitive behavioral therapy (CBT), implemented it in healthy children, and reported its effectiveness for health promotion.<br>
Objective: This study aims to examine the usefulness of the CBT app for improvement in depressive symptoms and QOL in children with mental health disorders.<br>
Methods: The participants were 115 children with mental health disorders (eg, school refusal, orthostatic hypotension, eating disorders, developmental disorders, among others) and aged 12‐18 years. The CBT app–based program comprised 1 week of psychoeducation followed by 1 week of self-monitoring. After reading story-like scenarios, participants created a self-monitoring sheet with 5 panels: events, thoughts, feelings, body responses, and actions. All participants received regular mental health care from physicians in addition to the app-based program. To evaluate the participants’ depressive symptoms and QOL, Patient Health Questionnaire for Adolescents (PHQ-9A), Depression Self-Rating Scale for Children (DSRS-C), and Pediatric Quality of Life Inventory (PedsQL) were measured at the beginning of the intervention, and at 2 and 6 months thereafter. Questionnaire for Triage and Assessment with 30 items (QTA30), and Rosenberg Self-Esteem Scale (RSES) were also used to measure their health and self-esteem. Participants were divided into 4 groups on the basis of the PHQ-9A score (above or below the cutoff; PHQ-9A≥5 or PHQ-9A<5) and completion or noncompletion of the CBT app–based program (app [+] or app [-]). The primary outcome was improvement in the DSRS-C score, and secondary outcomes were improvement in other psychometric scales including PedsQL, QTA30, and RSE. A paired-samples t test was used for statistical analysis. The Medical Ethics Committee of Fukuoka University Faculty of Medicine (approval U22-05-002) approved the study design.<br>
Results: There were 48, 18, 18, and 7 participants in the PHQ-9A≥5 app (+), PHQ-9A≥5 app (-), PHQ-9A<5 app (+), and PHQ-9A<5 app (-) groups, respectively. A total of 24 participants dropped out. No improvement in the DSRS-C score was observed in all groups. However, PedsQL scores improved significantly at 2 and 6 months in the PHQ-9A<5 app (+) group (t17=6.62; P<.001 and t17=6.11; P<.001, respectively). There was a significant positive correlation between the PHQ-9A scores and the number of self-monitoring sheets completed.<br>
Conclusions: The CBT app was useful for improving PedsQL scores of children with mental health disorders. However, a higher-intensity CBT program is necessary for more severely depressed children.<br>
Trial Registration: University Hospital Medical Information Network Clinical Trials Registry UMIN000046775; center6.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000053360No potential conflict of interest relevant to this article was reported.岡山大学経済学会Acta Medica Okayama2433-41465712025イノベーションの発生源研究の再検討120ENQiHuangDaijiFujii10.18926/OER/69001 本論文は，イノベーションの発生源に関する先行研究を振り返り，「いつ」「どこで」「誰によって」イノベーションが生み出されるのかを理論的に考察することを目的とする。考察の結果，「B to B」の文脈においては，イノベーションの発生源が企業ユーザーへ移行するメカニズムとして，取引コスト理論，期待利益仮説，情報粘着性の仮説，企業内部の独自能力（吸収能力），および外部の産業構造（製品アーキテクチャ・エコシステム）といった複数の要素からなる経済的合理性の観点から分析されていることが明らかになった。一方，「B to C」の文脈では，エンドユーザーがイノベーションに向かう動機として，「ニッチ市場に対するメーカーの消極的な対応」「創造的活動の楽しさ」「ユーザーコミュニティとの繋がり」「知識・スキルの向上」など多種多様な要素が存在し，中でも創造的活動の楽しさが根源的な動機づけの1つであると確認された。一方で，イノベーターを突き動かす心理的要因をブラックボックス化したまま放置することは，単なる知的好奇心の問題に留まらず，社会科学としての経営学にとっても重要な問題であると考えられる。今後のイノベーションの発生源研究においては，起業家をはじめとするイノベーター個人の心理的側面にいかに目を向け，創造的活動におけるポジティブな感情が働くメカニズムをイノベーションの発生メカニズムにいかに位置づけるか，その研究アプローチの提示が求められる。No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2041-17231512024Shoot-Silicon-Signal protein to regulate root silicon uptake in rice10712ENNaokiYamajiInstitute of Plant Science and Resources, Okayama UniversityNamikiMitani-UenoInstitute of Plant Science and Resources, Okayama UniversityToshikiFujiiInstitute of Plant Science and Resources, Okayama UniversityTomonoriShinyaInstitute of Plant Science and Resources, Okayama UniversityJi FengShaoState Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture & Forestry UniversityShotaWatanukiGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityYasunoriSaitohGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityJian FengMaInstitute of Plant Science and Resources, Okayama UniversityPlants accumulate silicon to protect them from biotic and abiotic stresses. Especially in rice (Oryza sativa), a typical Si-accumulator, tremendous Si accumulation is indispensable for healthy growth and productivity. Here, we report a shoot-expressed signaling protein, Shoot-Silicon-Signal (SSS), an exceptional homolog of the flowering hormone “florigen” differentiated in Poaceae. SSS transcript is only detected in the shoot, whereas the SSS protein is also detected in the root and phloem sap. When Si is supplied from the root, the SSS transcript rapidly decreases, and then the SSS protein disappears. In sss mutants, root Si uptake and expression of Si transporters are decreased to a basal level regardless of the Si supply. The grain yield of the mutants is decreased to 1/3 due to insufficient Si accumulation. Thus, SSS is a key phloem-mobile protein for integrating root Si uptake and shoot Si accumulation underlying the terrestrial adaptation strategy of grasses.No potential conflict of interest relevant to this article was reported.Royal Society of Chemistry (RSC)Acta Medica Okayama2050-748813232025A meta-linked isomer of ITIC: influence of aggregation patterns on open-circuit voltage in organic solar cells17720ENKaiWangThe Institute of Scientific and Industrial Research (SANKEN), The University of OsakaSeihouJinnaiThe Institute of Scientific and Industrial Research (SANKEN), The University of OsakaKaitoUesakaGraduate School of Natural Science & Technology, Okayama UniversityAkiraYamakataGraduate School of Natural Science & Technology, Okayama UniversityYutakaIeThe Institute of Scientific and Industrial Research (SANKEN), The University of OsakaImproving the open-circuit voltage (VOC) of organic solar cells (OSCs) remains an important challenge. While it is known that the energy levels at the donor/acceptor (D/A) interface affect the VOC, the impact of aggregation patterns on the energy levels at the D/A interface has not been fully elucidated. Herein, we focus on ITIC, a widely used acceptor in OSCs, and designed a meta-linked isomer of ITIC (referred to as im-ITIC) to alter molecular symmetry and modify substitution arrangements. Concentration-dependent 1H NMR spectra revealed that im-ITIC shows stronger aggregation behavior in solution. Single-crystal X-ray analysis showed that im-ITIC forms both tail-to-tail (J-aggregation) and face-to-face (H-aggregation) stacking modes, whereas ITIC exclusively forms tail-to-tail stacking. OSCs based on PBDB-T:im-ITIC showed a high VOC value of 1.02 V, which is 0.12 V higher than that of those based on PBDB-T:ITIC. Time-resolved infrared measurements revealed the lifetime of free electrons for the pristine and blend films. The energy levels of the charge transfer state (ECT) for PBDB-T:im-ITIC- and PBDB-T:ITIC OSCs were determined to be 1.57 and 1.39 eV, respectively, correlating with the VOC values. Theoretical calculations indicated that pronounced H-aggregation in im-ITIC increases the ECT compared with J-aggregation, contributing to the improved VOC. This study underscores the critical impact of molecular aggregation patterns on energy alignment and VOC enhancement, offering insights into molecular design for achieving high VOC in OSCs.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama0014-5793599132025Characterization of molecular mechanisms of CaMKKα/1 oligomerization19141924ENShunUenoyamaApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityHayatoNittaDepartment of Applied Chemistry and Biotechnology, Faculty of Engineering, Okayama UniversitySatomiOhtsukaApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasakiMagariApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityFutoshiSuizuDepartment of Medical Technology, Kagawa Prefectural University of Health SciencesHiroshiTokumitsuCalcium/calmodulin-dependent protein kinase kinase (CaMKK) is an activating kinase for calcium/calmodulin-dependent protein kinase type 1 (CaMKI), calcium/calmodulin-dependent protein kinase type IV (CaMKIV), RAC-alpha serine/threonine-protein kinase (PKB), and AMP-activated protein kinase (AMPK) that has been reported to form an active oligomer in cells. Glutathione S-transferase (GST) pulldown assay from the extracts of COS-7 cells expressing GST- and His6-CaMKKα/1 mutants showed that the C-terminal region containing the autoinhibitory and calmodulin (CaM)-binding sequence (residues 438–463) is required for CaMKKα/1 homo-oligomerization. This was confirmed by the fact that the GST-CaMKKα/1 C-terminal domain (residues 435–505) directly interacted with EGFP-CaMKKα/1 residues 435–505 as well as with wild-type CaMKKα/1. Notably, once oligomerized in cells, CaMKKα/1 is neither exchangeable between the oligomeric complexes nor dissociated by Ca2+/CaM binding. These results support stable oligomerization of CaMKK in the cells by intermolecular self-association of its C-terminal region containing a regulatory domain.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama2041-17231512024A high-protein diet-responsive gut hormone regulates behavioral and metabolic optimization in Drosophila melanogaster10819ENYutoYoshinariMetabolic Regulation and Genetics, Institute for Molecular and Cellular Regulation, Gunma UniversityTakashiNishimuraMetabolic Regulation and Genetics, Institute for Molecular and Cellular Regulation, Gunma UniversityTaishiYoshiiGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityShuKondoDepartment of Biological Science and Technology, Faculty of Advanced Engineering, Tokyo University of ScienceHiromuTanimotoGraduate School of Life Sciences, Tohoku UniversityTomoeKobayashiDivision of Molecular Genetics, Shigei Medical Research InstituteMakotoMatsuyamaDivision of Molecular Genetics, Shigei Medical Research InstituteRyusukeNiwaLife Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of TsukubaProtein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to protein deficiency are well-studied, there is a significant gap in our understanding of how organisms adaptively suppress excessive protein intake. In the present study, utilizing the fruit fly, Drosophila melanogaster, we discover that the peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells in response to a high-protein diet (HPD), is vital for suppressing overconsumption of protein. Gut-derived CCHa1 is received by a small subset of enteric neurons that produce short neuropeptide F, thereby modulating protein-specific satiety. Importantly, impairment of the CCHa1-mediated gut-enteric neuronal axis results in ammonia accumulation and a shortened lifespan under HPD conditions. Collectively, our findings unravel the crosstalk of gut hormone and neuronal pathways that orchestrate physiological responses to prevent and adapt to dietary protein overload.No potential conflict of interest relevant to this article was reported.MDPI AGActa Medica Okayama2079-77371452025Mutagenesis Targeting the S153 Residue Within the Transmembrane β-Hairpin of Mosquito-Larvicidal Mpp46Ab Affects Its Toxicity and the Synergistic Toxicity with Cry4Aa489ENTohruHayakawaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitySyunYamaokaGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMamiAsakuraGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMinakoHiranoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityToruIdeGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityWe constructed a library of Mpp46Ab mutants, in which S153 within the transmembrane β-hairpin was randomly replaced by other amino acids. Mutagenesis and subsequent primary screening yielded 10 different Mpp46Ab mutants in addition to the wild type. Remarkably, S153 was replaced with a more hydrophobic amino acid in most of the mutants, and the S153I mutant in particular exhibited significantly increased toxicity. Electrophysiologic analysis using artificial lipid bilayers revealed that the single-channel conductance and PK/PCl permeability ratio were significantly increased for S153I pores. This suggests that the formation of highly ion-permeable and highly cation-selective toxin pores increases the influx of cations and water into cells, thereby facilitating osmotic shock. In addition, the S153F, S153L, and S153I mutants exhibited significantly reduced synergistic toxicity with Cry4Aa. Electrophysiologic analysis showed that the S153F, S153L, and S153I mutants form toxin pores with a significantly reduced PK/PNa permeability ratio and a significantly increased PK/PCa permeability ratio compared to wild-type pores. Thus, our results suggest that pore formation is central to the insecticidal activity of Mpp46Ab and that the ion permeability of toxin pores is a potential indicator correlated with both toxicity and synergistic toxicity with other toxins.No potential conflict of interest relevant to this article was reported.Elsevier BVActa Medica Okayama2772-4174132025Photochemical internalization of mRNA using a photosensitizer and nucleic acid carriers100242ENHayakiMaemotoDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityRyoheiSuzakiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKazunoriWatanabeDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityKeijiItakaDepartment of Biofunction Research, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental UniversityTakashiOhtsukiDepartment of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversitymRNA has great potential for therapeutic applications because it can encode a variety of proteins and antigens, in addition to advantages over DNA in terms of gene expression without genomic integration, nuclear localization, or transcription. However, therapeutic applications of mRNA require safe and effective delivery into target cells. Therefore, we aimed to investigate photochemical internalization (PCI) as a promising strategy for delivering mRNA to target cells. In this strategy, mRNA is taken up into cells by endocytosis, accumulates in endosomes, and is released in a light-dependent manner from the endosomes using an endosome-accumulating photosensitizer, aluminum phthalocyanine disulfonate (AlPcS2a), in combination with nucleic acid carrier molecules. We compared the efficacy of various nucleic acid carriers, including branched polyethyleneimine (bPEI) and poly{N'-[N-(2-aminoethyl)-2-aminoethyl] aspartamide} (PAsp(DET)) under the same conditions for PCI-based mRNA delivery. Our results indicated that bPEI and PAsp(DET) at low N/P ratios exhibited efficient light-enhancement of mRNA expression by PCI with AlPcS2a. Notably, bPEI exhibited the highest light-dependent mRNA delivery among the carriers evaluated (including cationic polymers, cationic peptides, and lipids), whereas PAsp(DET) showed promise for clinical use because of its lower toxicity compared with bPEI. This PCI strategy allows effective cytosolic mRNA delivery at low N/P ratios, thereby reducing cationic carrier molecule-induced cytotoxicity. This method allows spatiotemporal control of protein expression and holds potential for novel light-dependent mRNA therapies. Overall, this study provided valuable insights into optimizing mRNA delivery systems for therapeutic applications.No potential conflict of interest relevant to this article was reported.日本機械学会Acta Medica Okayama2187-9761919462025ボールエンドミルの突き出し長さに応じた切削条件補正システムの開発24-00128ENHiroyukiKODAMAFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityYukiMORIYAGraduate school of Environmental, Life, Natural Science and Technology, Okayama UniversityTatsuoMORIMOTOGraduate school of Environmental, Life, Natural Science and Technology, Okayama UniversityKazuhitoOHASHIFaculty of Environmental, Life, Natural Science and Technology, Okayama UniversityIn the field of die and mold machining, determining appropriate cutting conditions is crucial. Factors such as tool geometry, machining path, work material characteristics, machining efficiency, and finishing accuracy must be taken into consideration. However, the current method of determining cutting conditions relies heavily on the intuition and experience of skilled engineers, and there is a need for a system to replace such knowledge. One of the critical factors affecting machining accuracy and efficiency is the tool overhang length, which is directly related to tool geometry. Unfortunately, there is no clear guideline for its determination. In a previous study, researchers developed a system to quickly derive cutting conditions using a data mining method and Random Forest Regression (RFR) applied to a tool catalog database. In this study, we constructed a new cutting condition compensation system based on the existing model, which accounts for the tool overhang length. The results of cutting experiments under high aspect ratio overhang lengths confirm that the correction coefficients proposed by the system are significant.No potential conflict of interest relevant to this article was reported.WileyActa Medica Okayama1526-99142662025Quantitative quality control of 3D water tank using image analysise70119ENYukiTanimotoDepartment of Radiology, NHO Kure Medical Center and Chugoku Cancer CenterKoheiSugimotoDepartment of Radiological Technology, Faculty of Health Science and Technology, Kawasaki University of Medical WelfareKazunobuKoshiDepartment of Radiology, NHO Fukuyama Medical CenterAkiraHiroshigeDepartment of Radiology, NHO Shikoku Cancer CenterShoheiYoshidaDepartment of Radiology, NHO Shikoku Cancer CenterYoshikiFujitaDepartment of Radiology, NHO Shikoku Cancer CenterAtsukiNakahiraDepartment of Radiology, NHO Shikoku Cancer CenterDaikiNakanishiDivision of Radiology, Department of Medical Technology, Kyushu University HospitalHirofumiHondaDepartment of Radiological Technology, Ehime University HospitalMasatakaOitaDepartment of Healthcare Science, Faculty of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityBackground and objective: Accurate beam data acquisition using three-dimensional (3D) water tanks is essential for beam commissioning and quality control (QC) in clinical radiation therapy. This study introduces a novel method for quantitative QC of the system, utilizing MV images and webcam videos. The stability of the motor drive speed and the positional accuracy of the fixture were evaluated under two measurement modes: “continuous mode” and “step-by-step mode.”<br>
Methods: A TRUFIX mounting system (PTW Freiburg Inc., Germany) was used to attach the center of the steel ball to its top, ensuring alignment with the water surface of the tank. To assess deviations from the radiation isocenter, MV images were acquired and compared with digitally reconstructed radiographs (DRRs). These evaluations were performed at different speed settings (slow, medium, and fast) using ET CT Body Marker (BRAINLAB Inc., USA) mounted on the drive unit. A webcam was utilized to capture the images, and custom-developed tracking software was employed to analyze deviations in driving speed and positional errors.<br>
Results: The mean error of the radiation isocenter was 0.37 ± 0.09 mm. As the motor drive speed increased, the discrepancy between the set speed and the actual speed observed in the analysis also became larger. In “continuous mode,” the deviation from the displayed value was greater than that observed in “step-by-step mode.”<br>
Conclusion: It is demonstrated that the proposed analysis method can quantitatively evaluate radiation isocenter misalignment, tank setup position deviation, and both the indicated drive speed values and their stability. At higher drive speeds, the “step-by-step mode” showed smaller deviations from the indicated values.No potential conflict of interest relevant to this article was reported.Springer Science and Business Media LLCActa Medica Okayama1865-03331812024Standardization of radiation therapy quality control system through mutual quality control based on failure mode and effects analysis7885ENYukiTanimotoGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityMasatakaOitaFaculty of Interdisciplinary Science and Engineering in Health Systems, Department of Healthcare Science, Okayama UniversityKazunobuKoshiDepartment of Radiology, NHO Fukuyama Medical CenterKiyoshiIshiwakiDepartment of Radiology, NHO Iwakuni Medical CenterFutoshiHiramatsuDepartment of Radiology, NHO Hamada Medical CenterToshihisaSasakiDepartment of Radiology, NHO Higashi-Hiroshima Medical CenterHirokiIseDepartment of Radiology, NHO Iwakuni Medical CenterTakashiMiyagawaDepartment of Radiology, NHO Kanmon Medical CenterTakeshiMaedaDepartment of Radiology, NHO Kochi National HospitalShinsukeOkahiraDepartment of Radiology, NHO Yamaguchi-Ube Medical CenterTakashiHamaguchiDepartment of Radiology, NHO Okayama Medical CenterTatsuyaKawaguchiDepartment of Radiology, NHO Shikoku Medical Center for Children and AdultsNorihiroFunadaDepartment of Radiology, NHO Hamada Medical CenterShuheiYamamotoDepartment of Radiology, NHO Fukuyama Medical CenterAkiraHiroshigeDepartment of Radiology, NHO Shikoku Cancer CenterYukiMukaiDepartment of Radiology, NHO Shikoku Cancer CenterShoheiYoshidaDepartment of Radiology, NHO Shikoku Cancer CenterYoshikiFujitaDepartment of Radiology, NHO Shikoku Cancer CenterAtsukiNakahiraDepartment of Radiology, NHO Shikoku Cancer CenterHirofumiHondaDepartment of Radiological Technology, Ehime University HospitalThe advancement of irradiation technology has increased the demand for quality control of radiation therapy equipment. Consequently, the number of quality control items and required personnel have also increased. However, differences in the proportion of qualified personnel to irradiation techniques have caused bias in quality control systems among institutions. To standardize the quality across institutions, researchers should conduct mutual quality control by analyzing the quality control data of one institution at another institution and comparing the results with those of their own institutions. This study uses failure mode and effects analysis (FMEA) to identify potential risks in 12 radiation therapy institutions, compares the results before and after implementation of mutual quality control, and examines the utility of mutual quality control in risk reduction. Furthermore, a cost-effectiveness factor is introduced into FMEA to evaluate the utility of mutual quality control.No potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025ウメの青果流通期間拡大に向けた台湾ウメの果実発育・成熟特性の調査および鮮度保持技術の適用ENTomoakiKASHIWAMOTOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Visible-Light-Responsive Nanocarbon Catalyst for Radical-Mediated Organic TransformationsENMD RAZU AHMEDGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025侵襲性歯周炎患者から分離した血液由来細胞外小胞のプロテオーム解析ENKanakoKODAMAGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Analysis of Protein Toxicity and Cellular Phenotypes Triggered by the Maximum Overexpression of Proteins in Yeast ENShotaroNAMBAGraduate School of Environmental, Life, Natural Science and Technology, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025消費者行動モデルを援用した効果検証モデルの検討－短期縦断調査による因果関係からの分析－ENMasashiKANDAGraduate School of Humanities and Social Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025肺腫瘍に対する定位放射線治療におけるターゲット設定の不確かさに関する研究ENDaikiNAKANISHIGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Study of the Brain Topological Processing Mechanisms in the Human Sensorimotor SystemENCHENYUWANGGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Study on neural mechanisms of attention effects on auditory-tactile multisensory processingENWEICHAOANGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Study on the Influence of Object Compliance on Softness and Pleasantness PerceptionENBINYUEGAOGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025高温超伝導SQUIDを用いた磁気ナノ粒子の磁気緩和ダイナミクス評価と応用探索ENKeiYAMASHITAGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Improved linear epitope-specific autoantibody discovery and quantitative assay systemENMireiDATE (MASUI)Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A Novel Drug Delivery System for Boron Neutron Capture Therapy (BNCT) in Cancer TheranosticsENABDUL BASITH FITHRONIGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Optimizing cell phone recycling for sustainable development: Integrating consumer behavior analysis, digital transformation strategies, and innovative incentive mechanismsENYuxinDUGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Risk Assessment for Heavy Rainfall-Induced Geohazards using Surrogate ModelsENSHIYINGZHENGGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Development of sediment microbial fuel cells to reduce CH4 and CO2 emissions from straw-amended paddy soilENADHENA TESFAU BEKELEGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025分布型光ファイバセンシング技術を用いた河川堤防の浸透流速および越水による破壊プロセスのモニタリングに関する研究ENHirokazuFUJIIGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025建設工程のDX化のための3次元データを活用した施工管理技術の研究ENKenzoNAGAEGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025ドローン測量による三次元点群データを活用した河川管理技術の開発ENNorikoTOMITAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025港湾におけるICT施工のためのモニタリング手法の研究ENIchiroTERUYAGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025マイクロ・ナノバブルを用いた集水井横ボーリング等閉塞物除去システムの研究ENKenzoSHINANOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025農業および建設分野のSociety5.0実現に向けたUAV測量技術の研究ENHikaruSANOGraduate School of Environmental and Life Science, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025組紐製造技術による繊維強化型人工筋肉の製作手法の確立と変位センシングの実現ENWEIHANGTIANGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025数理統計学と情報理論を用いた限定的な事前情報下での意思決定補助手法に関する研究ENShutoTANABASHIGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Implementations of Web Application Systems for Docker Image Generation and Flutter Programming Exercise AnswerENLYNN HTET AUNGGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025A Study of Hand Gestures for Controlling Video Games in Rehabilitation Exergame SystemENRADHIATUL HUSNAGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025炭素材料の内部状態解析に向けた固体NMR観測手法の革新と応用ENHidekaANDOGraduate School of Natural Science and Technology, Okayama universityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025環境中親電子物質によるDNAメチル化制御を介したケモカイン発現誘導機構ENTomokiTSUCHIDAGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025小胞体ストレスセンサー IRE1α に対する S-ニトロシル化阻害薬の同定とその薬効評価ENHarunaKUROGIGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Fusobacterium nucleatum LPSが誘導する近位尿細管SGLT2の過剰発現による糖尿病増悪機構ENAikoSEKIGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Effect of continuous sweet gustatory stimulation on salivary flow rate over timeENRankoYAMADAGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025塩化セチルピリジニウムと酸化グラフェン処理によるマスクの抗菌性向上ENTomotakaKOSHIMUNEGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.Acta Medica Okayama2025Human heart‑on‑a‑chip microphysiological system comprising endothelial cells, fibroblasts, and iPSC‑derived cardiomyocytesENYUNLIUGraduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNo potential conflict of interest relevant to this article was reported.老年人文学研究会Acta Medica Okayama2435-574732024投稿規程・奥付ENNo potential conflict of interest relevant to this article was reported.老年人文学研究会Acta Medica Okayama2435-574732024迷惑をかけたくない意識の形成―教育との関係―2254ENXiaofanDENG10.18926/JHG/68714No potential conflict of interest relevant to this article was reported.老年人文学研究会Acta Medica Okayama2435-574732024西田幾多郎臨終記121ENRyozoSUZUKIKoichiroYAMAMOTOMikakoOBIKA10.18926/JHG/68713No potential conflict of interest relevant to this article was reported.老年人文学研究会Acta Medica Okayama2435-574732024表紙・目次ENNo potential conflict of interest relevant to this article was reported.岡山大学理学部地球科学科Acta Medica Okayama1340-74143112025南西諸島の前弧域における付加体を含む堆積層のモデル化115ENMasanaoKOMATSUOkayama Gakuin UniversitySoheiURAKAMIFormerly Department of Earth Sciences, Okayama UniversityTaroOKAMOTODepartment of Earth and Planetary Sciences, School of Science, Institute of Science TokyoHiroshiTAKENAKADepartment of Earth Sciences, Okayama University10.18926/ESR/68676 We combine the recent seismic reflection profiles to construct a new seismic velocity model of the sedimentary layer incorporating the accretionary prism along the Ryukyu trench. In constructing the new model, we refer to the zoning (ZONE1 to ZONE4) identified by Okamura et al. (2017, Tectonophys.). The construction process consists of the following steps: First, we digitize either unconformities or VP=4 to 5 km/s lines as the seismic basement, whichever is more clearly identifiable. Second, the digitized thickness data of the sedimentary layer from the reflection profiles are geometrically modeled and interpolated to make the three-dimensional structure model. Finally, we supplement the external region of the constructed 3-D sedimentary model using the J-SHIS model provided by the NIED to complete the velocity structure model in the entire Ryukyu arc. The main features of our model are as follows: In ZONE1, off Ishigaki-jima island, the thick sedimentary layer extends about 50 km wide from the Ryukyu trench. In ZONE2, off Miyako-jima island, the thinner layer compared to the other zones is found near the trench, with a thin sedimentary terrace covering the area behind it. In ZONE3, off Okinawa-jima island, the sedimentary layer deepens as it approaches the trench. In ZONE4, off Tokara islands, the deepest layer among all zones is identified. We then conduct 3-D finite-difference simulations of seismic wave propagation using the new and the previous models to confirm the improvement of the new model. In the simulations, the effects of the accretionary prism along the Ryukyu trench on the seismic wave propagation are clearly identified.No potential conflict of interest relevant to this article was reported.岡山大学文明動態学研究所文化遺産マネジメント部門Acta Medica Okayama2758-962520232025岡山大学文明動態学研究所文化遺産マネジメント部門紀要2023ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025裏表紙・英文目次ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025投稿規程・奥付ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-3227520252024年度次世代医療機器開発人材育成プログラムBIZENデバイスデザインコースの取り組み4755ENTsuneakiTSUZUKICenter for Innovative Clinical Medicine, Okayama University HospitalDaisukeUCHIDACenter for Innovative Clinical Medicine, Okayama University HospitalToshioKISHIMOTOOrganization for Research Strategy and Development, Okayama UniversityYoshinariSENGOKUCenter for Innovative Clinical Medicine, Okayama University HospitalToshioKORENAGAOrganization for Research Strategy and Development, Okayama UniversityYuHITOBECenter for Innovative Clinical Medicine, Okayama University HospitalYasuyukiYOSHIBAAcademic Field of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityJunSAKURAICenter for Innovative Clinical Medicine, Okayama University Hospital10.18926/interdisciplinary/68626No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025イノベーションループの実践：2024年度先進病院実習報告と未来課題3945ENNahokoHARADAAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universitySatoshiTAKAHASHIAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityTomoakiMORIAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityHarukaHIKASAAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityKeisukeSHISHIDOAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityMasakiMAGARIAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityToyohikoWATANABEAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityJinWangAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama universityMizukiMORITAAcademic Field of Interdisciplinary Science and Engineering in Health Systems Health Sciences, Okayama university10.18926/interdisciplinary/68625No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025第16回 高度医療都市を創出する未来技術国際シンポジウム3537ENJiajiaYANGGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/68624No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025岡山大学中央図書館・個人文庫「杏村文庫」の調査：詩人との交友を中心に2933ENRyozoSUZUKIGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/68623No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025PrEP の利用に関する先行研究レビュー日本と中国を中心に1927ENDechengWangGraduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University10.18926/interdisciplinary/68622No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025関係的自律理論に基づいた終末期に関する意思決定支援の検討1118ENSumiyoSONOYAMAThe University of Shimane10.18926/interdisciplinary/68621In contemporary end-of-life care, it is difficult for patients to make decisions without the influence of society, family, and other factors. In many cases, patients have the capacity to make the decisions; nevertheless, they have difficulty expressing their own will because of the influence of their relationships and environment. Patient concerns about the burden of care and also the social and economic impacts on family members often hinder their use of imagination and decision-making. Therefore, this study has examined how patients with decision-making capacities could achieve autonomy under the influence of their relationships with their surroundings. The method of decision-making support provided by nurses to patients was examined using relational autonomy theory. Relational autonomy theory attempts to reconceptualize autonomy through feminists who criticize individualist theories of autonomy.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025知の探索時間についての企業の意思決定プロセス19ENTakeshiSHIMIZUGraduate School of Interdisciplinary Science and Engineering in Health Systems Okayama University10.18926/interdisciplinary/68620In order for companies to innovate through business co-creation, it is necessary to explore a wide range of external knowledge and technologies. However, there is no clear answer as to how much time should be spent for exploration. Under these circumstances, companies must take into account constraints such as the amount of management resources that can be invested, and make decisions about the time to spend for exploration. The purpose of this paper is to clarify the process of how companies that have introduced corporate accelerator program recognize the relationship between the program period and the results of business co-creation, and how they make decisions about the program period. We conducted a case study of several companies that have introduced corporate accelerator program in Japan. In addition, this paper established a hypothesis about decision-making about the time for exploration from case studies.No potential conflict of interest relevant to this article was reported.岡山大学大学院ヘルスシステム統合科学研究科Acta Medica Okayama2436-322752025表紙・目次ENNo potential conflict of interest relevant to this article was reported.MDPIActa Medica Okayama2075-44181562025Robustness of Machine Learning Predictions for Determining Whether Deep Inspiration Breath-Hold Is Required in Breast Cancer Radiation Therapy668ENWlla E.Al-HammadDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMasahiroKurodaRadiological Technology, Graduate School of Health Sciences, Okayama UniversityJamal, GhaidaAl JamalDepartment of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jordan University of Science and TechnologyMamikoFujikuraDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityRyoKamizakiRadiological Technology, Graduate School of Health Sciences, Okayama UniversityKazuhiroKurodaRadiological Technology, Graduate School of Health Sciences, Okayama UniversitySuzukaYoshidaDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYoshihideNakamuraDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMasatakaOitaGraduate School of Interdisciplinary Sciences and Engineering in Health Systems, Okayama UniversityYoshinoriTanabeRadiological Technology, Graduate School of Health Sciences, Okayama UniversityKoheiSugimotoRadiological Technology, Graduate School of Health Sciences, Okayama UniversityIrfanSugiantoDepartment of Oral Radiology, Faculty of Dentistry, Hasanuddin UniversityMajdBarhamDepartment of Dentistry and Dental Surgery, College of Medicine and Health Sciences, An-Najah National UniversityNouhaTekikiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMikiHisatomiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityJunichiAsaumiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityBackground/Objectives: Deep inspiration breath-hold (DIBH) is a commonly used technique to reduce the mean heart dose (MHD), which is critical for minimizing late cardiac side effects in breast cancer patients undergoing radiation therapy (RT). Although previous studies have explored the potential of machine learning (ML) to predict which patients might benefit from DIBH, none have rigorously assessed ML model performance across various MHD thresholds and parameter settings. This study aims to evaluate the robustness of ML models in predicting the need for DIBH across different clinical scenarios. Methods: Using data from 207 breast cancer patients treated with RT, we developed and tested ML models at three MHD cut-off values (240, 270, and 300 cGy), considering variations in the number of independent variables (three vs. six) and folds in the cross-validation (three, four, and five). Robustness was defined as achieving high F2 scores and low instability in predictive performance. Results: Our findings indicate that the decision tree (DT) model demonstrated consistently high robustness at 240 and 270 cGy, while the random forest model performed optimally at 300 cGy. At 240 cGy, a threshold critical to minimize late cardiac risks, the DT model exhibited stable predictive power, reducing the risk of overestimating DIBH necessity. Conclusions: These results suggest that the DT model, particularly at lower MHD thresholds, may be the most reliable for clinical applications. By providing a tool for targeted DIBH implementation, this model has the potential to enhance patient-specific treatment planning and improve clinical outcomes in RT.No potential conflict of interest relevant to this article was reported.MDPIActa Medica Okayama2075-44181562025Improving Diagnostic Performance for Head and Neck Tumors with Simple Diffusion Kurtosis Imaging and Machine Learning Bi-Parameter Analysis790ENSuzukaYoshidaDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMasahiroKurodaRadiological Technology, Graduate School of Health Sciences, Okayama UniversityYoshihideNakamuraDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYukaFukumuraDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYukiNakamitsuRadiological Technology, Graduate School of Health Sciences, Okayama UniversityWlla E.Al-HammadDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityKazuhiroKurodaRadiological Technology, Graduate School of Health Sciences, Okayama UniversityYudaiShimizuDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYoshinoriTanabeRadiological Technology, Graduate School of Health Sciences, Okayama UniversityMasatakaOitaGraduate School of Interdisciplinary Sciences and Engineering in Health Systems, Okayama UniversityIrfanSugiantoDepartment of Oral Radiology, Faculty of Dentistry, Hasanuddin UniversityMajdBarhamDepartment of Dentistry and Dental Surgery, College of Medicine and Health Sciences, An-Najah National UniversityNouhaTekikiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNurul N.KamaruddinDepartment of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMikiHisatomiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYoshinobuYanagiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityJunichiAsaumiDepartment of Oral and Maxillofacial Radiology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityBackground/Objectives: Mean kurtosis (MK) values in simple diffusion kurtosis imaging (SDI)-a type of diffusion kurtosis imaging (DKI)-have been reported to be useful in the diagnosis of head and neck malignancies, for which pre-processing with smoothing filters has been reported to improve the diagnostic accuracy. Multi-parameter analysis using DKI in combination with other image types has recently been reported to improve the diagnostic performance. The purpose of this study was to evaluate the usefulness of machine learning (ML)-based multi-parameter analysis using the MK and apparent diffusion coefficient (ADC) values-which can be acquired simultaneously through SDI-for the differential diagnosis of benign and malignant head and neck tumors, which is important for determining the treatment strategy, as well as examining the usefulness of filter pre-processing. Methods: A total of 32 pathologically diagnosed head and neck tumors were included in the study, and a Gaussian filter was used for image pre-processing. MK and ADC values were extracted from pixels within the tumor area and used as explanatory variables. Five ML algorithms were used to create models for the prediction of tumor status (benign or malignant), which were evaluated through ROC analysis. Results: Bi-parameter analysis with gradient boosting achieved the best diagnostic performance, with an AUC of 0.81. Conclusions: The usefulness of bi-parameter analysis with ML methods for the differential diagnosis of benign and malignant head and neck tumors using SDI data were demonstrated.No potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025岡山行政法実務研究会 研究会記録（第37回～第38回）143144ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025非法務部門で働くインハウス－内部監査、監査役スタッフ業務の魅力－113120ENYukoMinami10.18926/OLR/68569No potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025自動運転車と国際裁判管轄権・準拠法8195ENNaoeFuzisawa10.18926/OLR/68567No potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025自動運転技術の概要とその社会導入に向けた課題5780ENNaokiSuganuma10.18926/OLR/68566No potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025地域組織と連携した法務系人材育成システム・ 還元ルートの構築・強化130ENGoroSatoTakamotoSuzukiTetsuyaOhsakaTakafumiKobayashiNobuhiroOkamuraAkinariTakehisa10.18926/OLR/68564No potential conflict of interest relevant to this article was reported.岡山大学大学院法務研究科Acta Medica Okayama1881-1485262025表紙・目次ENNo potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671592025秦漢時代における官吏の処罰 ―「廢」と「免」を中心に―123141ENCongLIU10.18926/68544No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671592025共助による交通手段の確保に係る地域的対応 ―岡山県内を例に―6581ENKatsumiKATAOKA10.18926/68541No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671592025「やさしい日本語」における語彙難易度基準の再検討 ―J-CLIDによる多義語の細分類から―4564ENJianhuiLU10.18926/68540No potential conflict of interest relevant to this article was reported.岡山大学大学院社会文化科学研究科Acta Medica Okayama1881-1671592025入学情報へのアクセス性に関する研究 ―日本、東・東南アジア、英語圏の大学との比較から―114ENTakaoINAMORI10.18926/68537No potential conflict of interest relevant to this article was reported.岡山大学法学会Acta Medica Okayama0386-3050743-42025モントリオール条約に関する CJEU 判例の紹介312278ENF. MasudaNo potential conflict of interest relevant to this article was reported.American Association for Cancer Research (AACR)Acta Medica Okayama0008-54728562025Myeloid Cells Induce Infiltration and Activation of B Cells and CD4+ T Follicular Helper Cells to Sensitize Brain Metastases to Combination Immunotherapy10821096ENToshifumiNinomiyaDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityNaoyaKemmotsuDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityFumiakiMukoharaDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityMasakiMagariApplied Cell Biology, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityAiMiyamotoMedical Protein Engineering, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama UniversityYoukiUedaDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTakamasaIshinoDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityJojiNagasakiDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityTomohiroFujiwaraDepartment of Orthopaedic Surgery, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama UniversityHidetakaYamamotoDepartment of Pathology and Oncology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityHidetoshiHayashiDepartment of Medical Oncology, Kindai University Faculty of MedicineKotaTachibanaDepartment of Dermatology, Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama UniversityJojiIshidaDepartment of Neurological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityYoshihiroOtaniDepartment of Neurological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityShotaTanakaDepartment of Neurological Surgery, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityShinichiToyookaDepartment of General Thoracic Surgery, Breast and Endocrinological Surgery, Faculty of Medicine, Dentistry and Pharmaceutical Science, Okayama UniversityIsamuOkamotoDepartment of Respiratory Medicine, Graduate School of Medical Sciences, Kyushu UniversityYosukeTogashiDepartment of Tumor Microenvironment, Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama UniversityBrain metastasis is a poor prognostic factor in patients with cancer. Despite showing efficacy in many extracranial tumors, immunotherapy with anti–PD-1 mAb or anti–CTLA4 mAb seems to be less effective against intracranial tumors. Promisingly, recent clinical studies have reported that combination therapy with anti–PD-1 and anti–CTLA4 mAbs has a potent antitumor effect on brain metastasis, highlighting the need to elucidate the detailed mechanisms controlling the intracranial tumor microenvironment (TME) to develop effective immunotherapeutic strategies. In this study, we analyzed the tumor-infiltrating lymphocytes in murine models of brain metastasis that responded to anti–CTLA4 and anti–PD-1 mAbs. Activated CD4+ T follicular helper (TFH) cells with high CTLA4 expression characteristically infiltrated the intracranial TME, which were activated by combination anti–CTLA4 and anti–PD-1 treatment. The loss of TFH cells suppressed the additive effect of CTLA4 blockade on anti–PD-1 mAb. B-cell–activating factor belonging to the TNF family (BAFF) and a proliferation-inducing ligand (APRIL) produced by abundant myeloid cells, particularly CD80hiCD206lo proinflammatory M1-like macrophages, in the intracranial TME induced B-cell and TFH-cell infiltration and activation. Furthermore, the intracranial TME of patients with non–small cell lung cancer featured TFH- and B-cell infiltration as tertiary lymphoid structures. Together, these findings provide insights into the immune cell cross-talk in the intracranial TME that facilitates an additive antitumor effect of CTLA4 blockade with anti–PD-1 treatment, supporting the potential of a combination immunotherapeutic strategy for brain metastases.<br>
Significance: B-cell and CD4+ T follicular helper cell activation via BAFF/APRIL from abundant myeloid cells in the intracranial tumor microenvironment enables a combinatorial effect of CTLA4 and PD-1 blockade in brain metastases.No potential conflict of interest relevant to this article was reported.岡山大学教師教育開発センターActa Medica Okayama2186-1323152025岡山大学教師教育開発センターの活動状況の概要ENNo potential conflict of interest relevant to this article was reported.岡山大学教師教育開発センターActa Medica Okayama2186-1323152025小児がん患児に対する教育的支援とその研究動向341355ENYiwenCHENGraduate School of Education Doctoral Programs, Hyogo University of Teacher EducationMunehisaYOSHITOSHIGraduate School of Education, Okayama University10.18926/CTED/68503 本研究は、小児がん患児の教育的支援に関する研究の動向を明らかにし、今後の課題とその解決策を提起することを目的とした。「学習」、「自己管理」、「対人」、「心理」、「連携」の5つの視点から分析を行った。学習支援について、治療に伴う学習の遅れや集中力の低下に対し、個別の教育支援計画や体験的な学習、および遠隔教育の実施に期待されていた。自己管理支援では、患児が自身の病状や治療による副作用を理解し、自立的に管理できる能力を育成することが求められていた。対人支援について、患児の長期入院により、友人関係の構築が困難になり、入院中や復学後のコミュニケーションの支援が重要であることが確認された。心理支援においては、周囲への効果的ながん教育や大学生ボランティアによる支援が、患児の心理的安定を促すことが示された。最後に、学校と医療機関、保護者の連携不足が指摘されており、連携ツールの開発が求められている。No potential conflict of interest relevant to this article was reported.