Springer Science and Business Media LLC Acta Medica Okayama 1432-0584 105 5 2026 HLA-matched versus haploidentical donor transplantation with post-transplant cyclophosphamide: a study on behalf of the donor/source working group of the Japanese society for transplantation and cellular therapy 255 EN Yosuke Nakaya Department of Hematology, Osaka Metropolitan University Graduate School of Medicine Hirohisa Nakamae Department of Hematology, Osaka Metropolitan University Graduate School of Medicine Junichi Sugita Department of Hematology, Sapporo Hokuyu Hospital Junya Kanda Department of Hematology, Graduate School of Medicine, Kyoto University Yuta Hasegawa Department of Hematology, Hokkaido University Hospital Tetsuya Eto Department of Hematology, Hamanomachi Hospital Takahiro Fukuda Department of Hematopoietic Stem Cell Transplantation, National Cancer Center Hospital Naoki Kurita Department of Hematology, University of Tsukuba Hospital Nobuhiro Hiramoto Department of Hematology, Kobe City Medical Center General Hospital Koji Nagafuji Division of Hematology and Oncology, Department of Medicine, Kurume University HospitalDepartment of Hematology, Sapporo Hokuyu Hospital Shuichi Ota Department of Hematology, Sapporo Hokuyu Hospital Noboru Asada Department of Hematology and Oncology, Okayama University Hospital Toshihiko Ando Division of Hematology, Respiratory Medicine and Oncology, Department of Internal Medicine, Faculty of Medicine, Saga University Toshiro Kawakita Department of Hematology, NHO Kumamoto Medical Center Takashi Akasaka Department of Hematology, Tenri Hospital Yasuo Mori Hematology, Oncology & Cardiovascular medicine, Kyushu University Hospital Tomohiko Kamimura Department of Hematology, Harasanshin Hospital Makoto Onizuka Department of Hematology/Oncology, Tokai University School of Medicine Yoshiko Atsuta Japanese Data Center for Hematopoietic Cell Transplantation Hideki Nakasone Division of Hematology, Jichi Medical University Saitama Medical Center Post-transplant cyclophosphamide (PTCy) is now being increasingly applied to HLA-matched donor (MD) transplantation. Prior studies in Western countries have demonstrated that allogeneic hematopoietic cell transplantation (allo-HCT) employing PTCy yields better outcomes with HLA-matched donors (MDs) than with haploidentical donors (HIDs). However, the effect of HLA mismatch may differ among racial groups. We retrospectively analyzed adult patients with hematological malignancies who underwent their first allo-HCT with PTCy from MDs or HIDs registered to the Japanese registry database between 2013 and 2021. Among 63 (related, n = 33; unrelated, n = 30) and 1261 patients who received MD and HID allo-HCT with PTCy, 50 (related, n = 30; unrelated, n = 20) and 100 patients were assigned to MD and HID groups by 1:2 propensity score matching (PSM). The results showed that MD recipients had better neutrophil recovery (hazard ratio [HR], 1.48; 95% confidence interval [CI], 1.04–2.10; P = 0.031) and lower risk of non-relapse mortality (NRM) (HR, 0.19; 95% CI, 0.05–0.81; P = 0.024) than HID recipients. Multivariable analyses in the entire cohort before PSM confirmed these findings. Fatal infection was the primary cause of NRM in the HID group. This study is the first to demonstrate that, within a homogeneous Asian cohort, MD may have an advantage over HID in PTCy-based allo-HCT in facilitating neutrophil engraftment and reducing the risk of NRM. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 80 1 2026 Effective Treatment of Advanced Hepatocellular Carcinoma with Extensive Peritoneal Dissemination Using Lenvatinib 69 74 EN Shinya Wakatsuki Department of Obstetrics and Gynecology, Kochi Health Sciences Center Shinya Sakamoto Department of Gastroenterological Surgery, Kochi Health Sciences Center Akiko Ueno Department of Obstetrics and Gynecology, Kochi Health Sciences Center Takaomi Namba Department of Obstetrics and Gynecology, Kochi Health Sciences Center Yorito Yamamoto Department of Obstetrics and Gynecology, Kochi Health Sciences Center Manabu Matsumoto Department of Diagnostic Pathology, Kochi Health Sciences Center Jun Iwata Department of Diagnostic Pathology, Kochi Health Sciences Center Takehiro Okabayashi Department of Gastroenterological Surgery, Kochi Health Sciences Center Case Report 10.18926/AMO/70075 Patients with hepatocellular carcinoma (HCC) and extensive peritoneal dissemination generally have a poor prognosis and are often resistant to systemic therapy. We report the case of a 47-year-old woman with HCC and massive peritoneal dissemination who presented with malignant ascites requiring repeated cell-free and concentrated ascites reinfusion therapy and peritoneovenous shunt placement, as well as malignant pleural effusion requiring pleurodesis. Combined immunotherapy with durvalumab/tremelimumab was initiated;however, disease progression was observed after three treatment courses, prompting a switch to lenvatinib therapy. Two months after initiation of lenvatinib, CT imaging demonstrated complete disappearance of arterial enhancement in the primary hepatic lesion, along with reduction in the size of peritoneal dissemination nodules. Thirteen months after switching to lenvatinib (16 months after the initial diagnosis), the alpha-fetoprotein level continued to decrease, and the disease remained stable under treatment. Despite the extremely high tumor burden, lenvatinib achieved disease stabilization and symptomatic improvement. No potential conflict of interest relevant to this article was reported.

BMJ Acta Medica Okayama 2632-6140 7 1 2025 Reversible cerebral vasoconstriction syndrome in idiopathic multicentric Castleman disease under treatment with tocilizumab e000923 EN Naoya Kamimura Department of Neurology, Yokohama City University Medical Center Naohisa Ueda Department of Neurology, Yokohama City University Medical Center Katsuo Kimura Department of Neurology, Yokohama City University Medical Center Asami Nishikori Yasuharu Sato Hitaru Kishida Department of Neurology, Yokohama City University Medical Center Fumiaki Tanaka Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine Background Idiopathic multicentric Castleman disease (iMCD) is a rare polyclonal lymphoproliferative disorder characterised by systemic inflammation resulting from overproduction of interleukin 6 (IL-6). While iMCD primarily affects the lymph nodes and related tissues, it can also rarely involve the central nervous system.<br> Case presentation We report the case of a 58-year-old female patient with at least a 3-year history of iMCD, who experienced acute thunderclap headaches due to reversible cerebral vasoconstriction syndrome (RCVS). RCVS occurred 3 months after initiating treatment with tocilizumab, a humanised anti-IL-6 receptor monoclonal antibody, and was accompanied by focal cortical subarachnoid haemorrhage (SAH). Elevated IL-6 levels were found in both serum and cerebrospinal fluid. MR angiography revealed multiple diffuse stenotic lesions in the bilateral middle and posterior cerebral arteries, which, along with bilateral cerebral oedema, resolved within 3 months. The diffuse nature of the cerebral vasospasm and the presence of bilateral brain oedema suggested that cerebral vasospasm was due to RCVS rather than SAH.<br> Conclusions In patients with Castleman disease, RCVS may occur due to IL-6-dependent chronic cerebral vascular inflammation, either as a primary condition or as a complication of tocilizumab treatment. No potential conflict of interest relevant to this article was reported.

岡山大学大学院教育学研究科 Acta Medica Okayama 1883-2423 188 2025 食物アレルギーのセルフケアとピアサポートに関する発達段階別到達指標の提案 27 33 EN Yumiko SHINTAKU Graduate School of Education, Hyogo University of Teacher Education Hiroko KAMIMURA Faculty of Education, Okayama University Aiko TSUSHIMA Faculty of Education, Okayama University Atsushi TAKASE Faculty of Education, Okayama University 10.18926/bgeou/68411 　食物アレルギーのある子が健康で安全に過ごすためには，本人が必要なセルフケアを身につけることに加え，周囲の子が食物アレルギーについて理解し，適切に支援できる環境づくりが重要である。そのため，食物アレルギーに関する指導は，食物アレルギーのある子だけでなく，ない子に対しても発達段階に合わせて実施することが求められる。<br> 　そこで本研究は，各発達段階で子供に必要なセルフケアとピアサポートのスキルを包括的かつ具体的に整理し，指導の一助となる「食物アレルギーのセルフケアとピアサポートに関する発達段階別到達指標」を提案する。指標の作成時には，教員が疾患の特徴や子供の実態を踏まえて指導するため，学校生活における具体的なスキルとなるように表現を工夫した。 No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 76 5 2022 Idiopathic Pneumonia Syndrome Refractory to Ruxolitinib after Post-Transplant Cyclophosphamide-based Haploidentical Hematopoietic Stem Cell Transplantation: Lung Pathological Findings from an Autopsy Case 609 615 EN Ken Matsumoto Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Keigo Fujishita Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Masayuki Matsuda Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Satoshi Oka Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Yuka Fujisawa Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Toshi Imai Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Takuya Machida Department of Hematology and Blood Transfusion, Kochi Health Sciences Center Case Report 10.18926/AMO/64044 A 69-year-old Japanese man with acute leukemia received post-transplant cyclophosphamide-based haploidentical stem cell transplantation (PTCY-haplo-SCT) but was readmitted with dyspnea and ground-glass-opacities of the lungs. Bronchoscopy showed inflammatory changes with no signs of infection. He received steroids but required intubation as his condition deteriorated. In addition to antithymocyte globulin and cyclophosphamide, we administered ruxolitinib but failed to save him. Autopsy findings revealed fibrotic nonspecific interstitial pneumonia (NSIP) without evidence of organizing pneumonia or infection. Thus, we diagnosed idiopathic pneumonia syndrome (IPS). As far as our knowledge, this is the first case of IPS with NSIP histology after PTCY-haplo-SCT. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 76 3 2022 Analysis of Immunity against Measles, Mumps, Rubella, and Varicella Zoster in Adult Recipients of Allogeneic Hematopoietic Stem Cell Transplantation: A Single-Center Experience 247 253 EN Shohei Yoshida Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Nobuharu Fujii Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Chihiro Kamoi Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Wataru Kitamura Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hideaki Fujiwara Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Noboru Asada Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Hisakazu Nishimori Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Keiko Fujii Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Ken-ichi Matsuoka Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Yoshinobu Maeda Department of Hematology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Original Article 10.18926/AMO/63718 Vaccine-preventable disease (VPD) infections are more severe in immunocompromised hosts. Vaccination against measles, mumps, rubella, and varicella zoster (VZV) (MMRV) is therefore recommended for hematopoietic stem cell transplantation (HCT) recipients. However, studies on adult HCT recipients with VPD infections are limited. At our institution, we have systematically conducted serological MMRV tests as a part of check-up examinations during long-term follow-up (LTFU) after HCT since 2015. This retrospective study aimed to evaluate changes in the serostatus between before and 2 years after allogeneic HCT. Among 161 patients, the pre-transplant seropositivity was 82.7% for measles, 86.8% for mumps, 84.2% for rubella, and 94.3% for VZV. Among 56 patients who underwent LTFU including serological MMRV tests at 2 years after HCT, the percentages maintaining seroprotective antibody levels for measles, mumps, rubella and VZV were 71.5% (40/56), 51.8% (29/56), 48.2% (27/56), and 60.7% (34/56), respectively. Vaccination was recommended for 22 patients, and 12 were vaccinated. Among the 12 vaccinated patients, rates of seroconversion were examined in 2-6 patients for each of the four viruses. They were 100% (3/3) for measles, 33.3% (1/3) for mumps, 50% (3/6) for rubella, and 0% (0/2) for VZV. Further studies are warranted to clarify the effect of vaccination in adult HCT recipients. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 75 2 2021 Awareness of Complications of Dental Treatment in Patients Treated with Drugs Affecting the Immune System : A Nationwide Questionnaire Survey of Dental Practitioners in Japan 115 123 EN Nishizaki Hitomi Department of Critical Care Medicine and Dentistry, Graduate School of Dentistry, Kanagawa Dental University Morimoto Yoshinari Department of Critical Care Medicine and Dentistry, Graduate School of Dentistry, Kanagawa Dental University Shin-ichi Yamada The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Hiroshi Kurita The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Akira Tanaka The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Akira Yamaguchi The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Masaru Miyata The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Hiromasa Yoshikawa The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Souichi Yanamoto The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Yutaka Imai The survey and research-planning committee, Japanese Society for Dentistry of Medically Compromised Patient Original Article 10.18926/AMO/61877 The aim of this study was to investigate the awareness and experience, among dental practitioners, of adverse events resulting from dental treatment of patients undergoing therapy with drugs that affect the immune system [angiogenesis inhibitors, biological agents, immunosuppressants, and disease-modifying anti-rheumatic drugs (DMARDs)]. For this purpose, a nationwide questionnaire survey was conducted. Questionnaires were sent to 2,050 dentists, of which 206 (10.1%) were completed and returned. The results showed that most dentists were aware of complications associated with dental treatment of patients treated with drugs that affect the immune system, and about half had actually experienced such complications. Delayed wound healing, osteonecrosis of the jaw (ONJ), and postoperative infections were reported. Whereas approximately 50% of dentists did not discontinue the drugs during dental treatment, about 18% did. During temporary drug discontinuation, some patients experienced aggravation of the primary disease, such as worsening of rheumatism, growth of tumors, and rejection reactions of transplanted organs. As for medical cooperation, only less than half of the dentists were asked for oral hygiene management by a physician prior to starting the drug treatment. Prospective studies are needed because evidence for dental treatments in patients treated with these drugs remains limited. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 74 4 2020 Successful Vancomycin Dose Adjustment in a Sepsis patient with Bacterial Meningitis Using Cystatin C 365 370 EN Masayuki Chuma Clinical Research Center for Developmental Therapeutics, Tokushima University Hospital Masateru Kondo Department of Pharmacy, Tokushima University Hospital Yoshito Zamami Department of Pharmacy, Tokushima University Hospital Kenshi Takechi Clinical Research Center for Developmental Therapeutics, Tokushima University Hospital Mitsuhiro Goda Department of Pharmacy, Tokushima University Hospital Naoto Okada Department of Pharmacy, Tokushima University Hospital Akitomo Shibata Department of Pharmacy, Saiseikai Kumamoto Hospital Mizuho Asada Department of Pharmacy, Medical Hospital, Tokyo Medical and Dental University Jun Oto Department of Emergency and Critical Care Medicine, Tokushima University Graduate School of Biomedical Sciences Hiroaki Yanagawa Clinical Research Center for Developmental Therapeutics, Tokushima University Hospital Keisuke Ishizawa Department of Pharmacy, Tokushima University Hospital Case Report 10.18926/AMO/60376 Cystatin C-guided vancomycin (VCM) dosing is useful in critically ill patients. Its usefulness in septic patients with bacterial meningitis remains unknown, as there are no published reports. In this study, we sought to clarify its benefit. Cystatin C was used to guide VCM dosing in a septic bacterial meningitis patient with normal kidney function, according to therapeutic drug monitoring (TDM). Using cystatin C, the Bayesian method-based TDM made optimal VCM dosing possible, and decreased the predicted error (4.85 mg/L) compared to serum creatinine (16.83 mg/L). We concluded TDM of VCM using cystatin C can be considered in sepsis patients with bacterial meningitis with normal kidney function. No potential conflict of interest relevant to this article was reported.

Elsevier Acta Medica Okayama 09492658 2020 Reference values for the locomotive syndrome risk test quantifying mobility of 8681 adults aged 20–89 years: A cross-sectional nationwide study in Japan EN Keiko Yamada Departments of Sensory & Motor System Medicine, Faculty of Medicine, The University of Tokyo Yoichi M. Ito Department of Statistical Data Science, The Institute of Statistical Mathematics Masao Akagi Department of Orthopedic Surgery, Kindai University Hospital Etsuo Chosa Department of Orthopaedic Surgery, University of Miyazaki Takeshi Fuji “Locomo Challenge!” Promotion Council Kenichi Hirano Hirano Orthopaedics Clinic Shinichi Ikeda Department of Orthopaedic Surgery, Oita University, Hideaki Ishibashi “Locomo Challenge!” Promotion Council Yasuyuki Ishibashi Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine Muneaki Ishijima “Locomo Challenge!” Promotion Council Eiji Itoi Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine Norimasa Iwasaki “Locomo Challenge!” Promotion Council Ryoichi Izumida “Locomo Challenge!” Promotion Council Ken Kadoya Department of Advanced Medicine for Locomotor System, Faculty of Medicine and Graduate School of Medicine, Hokkaido University Masayuki Kamimura Department of Orthopaedic Surgery, Tohoku University Graduate School of Medicine Arihiko Kanaji “Locomo Challenge!” Promotion Council Hiroyuki Kato Department of Orthopaedic Surgery, Shinshu University School of Medicine Shunji Kishida “Locomo Challenge!” Promotion Council Naohiko Mashima Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine Shuichi Matsuda Department of Orthopaedic Surgery, Kyoto University Graduate School of Medicine Yasumoto Matsui Center for Frailty and Locomotive Syndrome, National Center for Geriatrics and Gerontology Toshiki Matsunaga Department of Rehabilitation Medicine, Akita University Hospital Naohisa Miyakoshi Department of Orthopedic Surgery, Akita University Graduate School of Medicine Hiroshi Mizuta Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University Yutaka Nakamura Saiseikai Shonan Hiratsuka Hospital Ken Nakata Medicine for Sports and Performing Arts, Osaka University Graduate School of Medicine Go Omori Department of Sports and Health, Faculty of Health and Science, Niigata University of Health and Welfare Koji Osuka Osuka Clinic Yuji Uchio Department of Orthopaedic Surgery, Shimane University Kazuteru Ryu Kanai Hospital Nobuyuki Sasaki Sasaki Orthopedic and Anesthesiology Clinic Kimihito Sato “Locomo Challenge!” Promotion Council Masuo Senda Okayama University Hospital, Division of Physical Medicine and Rehabilitation Akihiro Sudo Department of Orthopaedic Surgery, Mie University Graduate School of Medicine Naonobu Takahira Department of Rehabilitation, Kitasato University School of Allied Health Sciences Hiroshi Tsumura Department of Orthopaedic Surgery Satoshi Yamaguchi “Locomo Challenge!” Promotion Council Noriaki Yamamoto Nigata Rehabilitation Hospital Kozo Nakamura “Locomo Challenge!” Promotion Council Ohe Takashi “Locomo Challenge!” Promotion Council, T Background<br/> The locomotive syndrome risk test was developed to quantify the decrease in mobility among adults, which could eventually lead to disability. The purpose of this study was to establish reference values for the locomotive syndrome risk test for adults and investigate the influence of age and sex.<br/> Methods<br/> We analyzed 8681 independent community dwellers (3607 men, 5074 women). Data pertaining to locomotive syndrome risk test (the two-step test, the stand-up test, and the 25-question geriatric locomotive function scale [GLFS-25]) scores were collected from seven administrative areas of Japan.<br/> Results<br/> The reference values of the three test scores were generated and all three test scores gradually decreased among young-to-middle-aged individuals and rapidly decreased in individuals aged over 60 years. The stand-up test score began decreasing significantly from the age of 30 years. The trajectories of decrease in the two-step test score with age was slightly different between men and women especially among the middle-aged individuals. The two physical test scores were more sensitive to aging than the self-reported test score.<br/> Conclusion<br/> The reference values generated in this study could be employed to determine whether an individual has mobility comparable to independent community dwellers of the same age and sex. No potential conflict of interest relevant to this article was reported.

岡山大学全学教育・ 学生支援機構 Acta Medica Okayama 2432-9665 4 2019 大学入学者選抜改革に対応する高校トライアルの概要について : 国立六大学連携コンソーシアム教育連携機構入試専門部会事業 149 167 EN Ichiro ISHII Kazuyuki HARADA Hiroko KAMIMURA Mitsuji MONTA 10.18926/58043 6 大学連携事業として，評価したい資質について掘り下げ質問を行う構造化面接と，面接に代わる筆記試験であるペーパーインタビューについて，県内の高等学校の協力を得て2019 年度にトライアルを行った。評価した資質は「新たなこと追究しようとする態度」と「協働して取り組む態度」であり，まず，構造化面接とペーパーインタビューの結果を本学の教員が評価し，その結果を高校教員が個々の受験生に抱いている日常的評価と比較した。トライアルの結果，構造化面接の有効性ならびに，受験者全員に面接を課すことが，時間的，人員的に実施困難な入試におけるペーパーインタビューの有効性が確認された。同時に，測ろうとする資質によっては，面接との評価結果が異なる部分があることも明らかとなった。 No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 109 2020 酸性鉱山廃水の 効果的な生物的処理プロセスの開発 29 36 EN Kazuo Kamimura Graduate School of Environmental and Life Science, Okayama University Tadayoshi Kanao Graduate School of Environmental and Life Science, Okayama University Acid mine drainage (AMD) is acidic and generally enriched with iron, aluminum, sulfate and heavy metals, such as lead and cadmium. AMD is a growing problem of emerging concern that cause detrimental effects to the environment and living organisms. Yanahara mine in Misaki Town, Okayama, Japan, had mainly produced pyrite for sulfuric acid manufacture. Although it was closed in 1991, AMD is being generated from the mine now. A passive treatment based on the biological oxidation of ferrous iron is a promising strategy for AMD remediation. AMD from Yanahara mine is treated in a plant using iron-oxidizing bacteria, Acidithiobacillus ferrooxidans and Ferrovum spp. The AMD generation continues for several centuries with dramatic consequences on the receiving environments. Therefore, the development of sustainable and cost effective treatment process is required. A development of the effective biological treatment process with an iron oxidation reactor operated at pH 3.5 is described in this report. Economic aspects are also discussed. No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 7 2017 ICT を活用した模擬保健指導における能動的学修の取り組み : 実践的指導力の育成をめざした授業の開発と改善 167 174 EN Aki Kano Faculty of Nursing, Seisen University Kayo Takahashi Kurashiki Sakuyo University Hiroko Kamimura Graduate School of Education, Okayama University Hokuma Munakata Graduate School of Education, Okayama University 10.18926/CTED/54944 　養護教諭の職務の重要な柱である保健指導の実践的指導力を育成するために，養成段階においても能動的学修を推進する必要がある。そこで教職科目「教育方法論」において，ICT を活用した能動的学修として模擬保健指導に取り組んだ。授業では，子どもの実態や健康課題に基づいて指導案を作成し保健指導の設計・実施・評価を一連の流れとしたグループ活動を展開した。平成25 年度から平成27 年度の3 年間の授業開発・改善の結果，「非常に到達目標を達成できた」とする学生は，平成25 年度の18.2%から平成27 年度は26.7%に増加した。「授業目標や内容が非常に理解できた」のは31.8%から60%，「教材が授業内容の理解や自宅学習に非常に役立った」のは40.9%から60%，2時間以上予習や課題にかける学生も18.2%から40%に増加した．保健指導の実践的指導力を育成し効果的な授業を可能とするためには，模擬保健指導をICT を活用した能動的学修にすることは有効といえる。 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 129 1 2017 水素水は怪しい水でしょうか？ 9 15 EN Atsunori Nakao Department of Emergency and Critical Care Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences No potential conflict of interest relevant to this article was reported.

岡山大学大学院教育学研究科 Acta Medica Okayama 1883-2423 161 2016 養護教諭に必要とされるフィジカルアセスメント ― 保健室でみられる原因を根拠とした提案 ― 25 33 EN Yukari Mimura Mutsumi Matsueda Atsuko Kasai Tomiko Nakashita Nobuko Sato Ai Yamauvhi Aiko Tsushima Hiroko kamimura 10.18926/bgeou/54229 学校において適切な救急処置を行うために，養護教諭には緊急度・重症度の判断が求められている。しかし，養護教諭に必要な救急処置の範囲は明確にされておらず，困難を感じる要因となっている。近年，救急度・重症度の判断のためにフィジカルアセスメントの重要性が認識されているが，これについても必要な項目が明確になっていないのが現状である。そこで，本研究では，養護教諭が保健室で経験する疾患を根拠として，養護教諭に必要なフィジカルアセスメントを明らかにすることを試み，「息苦しい」の訴えに対して必要な項目を示した。 No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 5 2015 養護教育実践研究における大学院生の学び : 研究的視点を持って保健室経営に参画するために 164 171 EN Sakie Hoshino Akemi Katta Keiko Nakamura Hiroe Honda Ikumi Furukawa Hiroko Kamimura Hokuma Munakata 10.18926/CTED/53247 　「発達支援研究特論�X（養護教育実践研究）」は，大学で学んだ理論を学校現場で実証し，養護実践研究を行う 能力を身に付けることを目標にした授業科目である。大学院生３名の附属小・中学校における取組と課題の検討 を通じて，学びと保健室経営への参画について考察した。その結果，大学院生は異なる事例の検討を通じて，様々 な角度から課題を発見し，その対策を示すことができた。また，事例検討の過程で，自分自身が一定の役割を果 たすこと，より広い視野を持つ必要性，一人の養護教諭としての自覚をより強く持つ必要があること等に気付く ことができた。大学教員の課題として，養護実践のふりかえりの頻度を増やし，よりきめ細かくフィードバック すること等が挙げられた。このような取組は養護教諭としての資質能力の向上に有用であると考えられた。今後 は大学院生が自覚的，主体的に養護実践に取り組み，学びを検討し，共有する機会の充実が求められる。 No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 5 2015 養護教諭が推進する組織的で継続可能な小学校の喫煙・飲酒・薬物乱用防止教育プログラムの開発と実践 139 148 EN Yoshiko Ueno Takae Nishimura Sayuri Hosaka Masae Yamamoto Rie Akasaka Ayumi Arimatu Harue Fujii Makoto Yuguchi Kanako Torigoe Marika Oonishi Yumiko Nanba Ayako Mori Hiroe Washida Keiko Nishimoto Yuko Hino Tomoko Matumoto Tomoko Ishihara Mariko Todo Kumiko Yamamoto Natuki Sadamori Nao Yamagami Kanako Okamoto Hiromi Matsuo Shizuka Kobayashi Kanako Hayashi Nozomi Mizushima Ken-ichi Sekiyama Takashi Fukushima Hiroko Kamimura Kayo Takahashi 10.18926/CTED/53244 　学校における喫煙・飲酒・薬物乱用防止教育の充実には，問題行動が顕在化する中学校期だけでなく小学校期 における指導の推進が重要であり，系統的な指導計画を立て，指導者や時間の確保，教材作成などに組織的に取 組み，継続可能なプログラム開発を行う必要がある。そこで，地区内12 校の養護教諭が協働して，発達段階に応 じた系統的・組織的かつ継続可能な地区共通の指導計画を開発し，各校の教育課程・年間計画に位置付けた実践 研究を行った。その結果，指導計画の実施状況は，小学校11 校中，学級活動10 校，ミニ保健指導10 校，長期 休業前指導6 校，広報活動9 校となり，特別支援学校1 校では広報活動のみを行うことができた。小学校におけ る喫煙・飲酒・薬物乱用防止教育の推進には，学校保健活動の中核的役割を担う養護教諭が専門性を活かし協働 して，系統的な指導計画を各校の教育課程に位置付け組織的で継続可能なプログラムとする取組が有効であった。 No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 5 2015 教育実習における附属学校園養護教諭の取組 : 学校保健活動を遂行する教員を養成するために 129 138 EN Mio Kamikawa Hiroe Honda Akie Kitahara Ikumi Furukawa Hiroko Kamimura Takehiko Ito Yoshihisa Sumino Yukari Mimura 10.18926/CTED/53243 　子どもの多様な健康課題に対応して，学校における安全・安心な環境の確保や子どもの心身の健康を守り，は ぐくむことのできる体制構築が求められる今日，教育実習等においては，実習生を学校保健に対する関心・理解 を高め，子どもの健康・安全な学校生活を考慮した教育活動を展開できる教員として育成することが重要となる。 そこで，教育実習等の機会に附属学校園養護教諭が，学校保健活動を遂行できる教員養成を目的とした講義・演 習を実施した。その結果，実習生は学校保健に対する認識を高めることができた。附属学校園養護教諭が教員養 成に積極的に参画することは，学校保健活動を遂行できる教員養成に果たす役割が大きいといえる。 No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 2186-7755 104 2015 Characterization of a putative chromosome segregation and condensation protein (ScpB) in an acidophilic iron‒oxidizing bacterium Acidithiobacillus ferrooxidans 5 12 EN Kazuo Kamimura Nozomu Nagata Mei Kikumoto Sultana Sharmin Satoshi Wakai Tadayoshi Kanao 　Acidithiobacillus ferrooxidans is one of the most widely used microorganisms in bioleaching operations to recover copper from low-grade copper sulfide. This bacterium uses ferrous iron and reduced inorganic sulfur compounds (RISCs) as energy sources. Transcriptions of genes thought to be involved in the oxidation of RISCs have been known to be highly activated in A. ferrooxidans cells grown on RISCs, while transcriptions of genes involved in the iron oxidation were repressed in the cells grown on RISCs. A gene encoding a putative chromosome segregation and condensation protein (ScpB) with a helix-turn-helix motif was found in the upstream region of sulfide : quinone oxidoreductase gene, whose expression was up-regulated in cells grown in sulfur and tetrathionate. A semi-quantitative PCR analysis using cDNA prepared from iron-, sulfur-, or tetrathionate-grown cells revealed that the transcription of scpB was up-regulated in cells grown on sulfur or tetrathionate as the energy source. Electrophoretic mobility shift assays were employed to examine whether the ScpB functions as a transcription factor. The result indicated that the recombinant His-tagged ScpB protein was able to nonspecifically bind in vitro to DNA. This is the first report on a direct association of ScpB with DNA. No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 4 2014 附属小学校における保健指導の展開 : 指導の実際を養護実習にいかす 133 142 EN Hiroe Honda Kazuaki Kitahara Mihoko Mito Hiroko Kamimura Hokuma Munakata Kayoko Miyamoto Shinichiro Monden 10.18926/CTED/52298 　附属小学校において，日頃の保健室で児童との関わりから気づいた課題と体育科保健領域とのつながりを意識しながら，歯・口を題材に，からだの発育発達についての保健指導を行った。同時に，養護教諭養成をになう附属小学校として，保健指導に取り組む際のプロセスを明確にすることを目的に，実践の振り返りを行った。指導計画の作成から実践までの過程を，児童の学び，授業者の反省，学級担任の評価についてまとめ，保健指導の展開にいかす視点を検討したので報告する。 No potential conflict of interest relevant to this article was reported.

岡山大学教師教育開発センター Acta Medica Okayama 2186-1323 3 2013 附属中学校における学校保健委員会の取組み―養護実習への活用を視野に入れて― 152 161 EN Yasuko Ota Hiroko Kamimura Hokuma Munakata Kayoko Miyamoto Shinichiro Monden 10.18926/CTED/49516 　岡山大学教育学部附属中学校における学校保健委員会の取組み，および学校保健委員会の資料の養護実習への活用について報告する。附属中学校の学校保健委員会は，�@学校保健活動の全体像が理解されること，�A課題が明確になる資料を工夫すること，�B関係者の共通理解が図られること，の3つの視点から取組まれており，このことが日々の学校保健活動に対する有効な施策を協議するために重要であった。学校保健委員会の資料は同時に，養護実習において学生が学校保健活動を理解するための教材として活用できる。 No potential conflict of interest relevant to this article was reported.

Springer Japan Acta Medica Okayama 1431-0651 15 3 2011 Characterization of an OmpA-like outer membrane protein of the acidophilic iron-oxidizing bacterium, Acidithiobacillus ferrooxidans 403 410 EN Mohammed Abul Manchur Mei Kikumoto Tadayoshi Kanao Jun Takada Kazuo Kamimura An OmpA family protein (FopA) previously reported as one of the major outer membrane proteins of an acidophilic iron-oxidizing bacterium Acidithiobacillus ferrooxidans was characterized with emphasis on the modification by heat and the interaction with peptidoglycan. A 30-kDa band corresponding to the FopA protein was detected in outer membrane proteins extracted at 75A degrees C or heated to 100A degrees C for 10 min prior to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). However, the band was not detected in outer membrane proteins extracted at a parts per thousand currency sign40A degrees C and without boiling prior to electrophoresis. By Western blot analysis using the polyclonal antibody against the recombinant FopA, FopA was detected as bands with apparent molecular masses of 30 and 90 kDa, suggesting that FopA existed as an oligomeric form in the outer membrane of A. ferrooxidans. Although the fopA gene with a sequence encoding the signal peptide was successfully expressed in the outer membrane of Escherichia coli, the recombinant FopA existed as a monomer in the outer membrane of E. coli. FopA was detected in peptidoglycan-associated proteins from A. ferrooxidans. The recombinant FopA also showed the peptidoglycan-binding activity. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 67 3-4 1955 Sinomenineのモルモツト過敏症に対する防禦効果について 873 881 EN Yukio Kamimura Ryosaku Nishiyama Anti-anaphylactic effect of sinomenine was studied in guinea-pigs sensitized with bovine serum. Fairly constant severity of shock was obtained in the control animals by determining challenging dose based on Prof. Ogata's so-called binding zone: the highest mutiplication of the antigen giving positive reaction with the highest dilution of the antibody. Standard dose for challenge was calculated by the formula. body weight (g)×1/13×1/binding zone. Sinomenine was effective in preventing the guinea-pig's anaphylactic shock, but not so remarkably. Five daily subcutaneous injections of 50mg/kg sinomenine were required to prevent death from the active anaphylactic shock induced by the standard antigen dose. Under this pretreatment only about half number of the animals have survived death from the passive anaphylaxis shocked by the same antigen dose. Therefore, sinomenine seemed to be less effective in preventing guinea-pig anaphylaxis than canine anaphylaxis, against which marked protection was observed by one of us (R. N.) In the guinea-pig injected sinomenine 50mg/kg for 5 consecutive days, histamine content of skin of the head region was reduced by ca. 26%, but no reduction was observed in the abdominal skin and lung tissues. This degree of histamine release was much less than in the dog. The guinea-pig became more susceptible to histamine after the repeated injection of sinomenine, but this phenomenon is not specific to this species. Sinomenine did not inhibit the precipitin reaction in the guinea-pig anaphylaxis. From these, it may be concluded that the protective effect of sinomenine against guinea-pig anaphylaxis is less marked than against canine anaphylaxis and this is chiefly due to lesser ability of sinomenine in releasing histamine in this species. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 69 11 1957 犬の皮膚細切組織による薬物のHistamine遊離に関する定量的研究 2853 2868 EN Kenji Tasaka With a view to explore and make a comparative study of various drugs as regards their activity in liberating skin histamine a quantitative determination method of in vitro histamine release from chopped dog skin has been devised and a study on the conditions influencing histamine release has been carried out. Abdominal skin was removed from dog and cut in a fixed thickness by a chopper of my own contrivance, consisting of razor blades placed at given spaces and after eliminating the majority of histamine spontaneously diffused out by immersing in Tyrode solution, the skin pieces were shaked in a glass tube containing drug solution to be tested along with a glass bead for one hour at 37°C, and then the histamine content diffusing out in the drug solution and residual histamine in the tissue were determined separately. The histamine releasing activity of drugs depends on the thickness of tissue pieces, temperature, and pH of the solution. In the case of thinner tissue pieces the rate of histamine release either by releaser solution or of spontaneous release proved to be greater. Below 45°C, the rise in temperature enhanced the release by a releaser more markedly than spontaneous release. At 60°C, however, all histamine in tissues diffused out within 60 minutes in the Tyrode solution not containing releaser. In the isotonic buffer solution of pH 6-8 spontaneous release proved to be least, while at the pH either more acidic or basic the rate of release increased. On the alkaline side the effect of releaser of alkaloid salt increased to a greater degree than spontaneous release. This fact suggests that the penetration of alkaloids into the cell wall takes place readily in the form of base. When the tissue is acted upon by the Tyrode solution or by 0.9% saline solution of 78 drugs tested, at 37°C for 60 minutes, these listed in the following released histamine from chopped skin rather markedly: Sinomenine, quinine, quinidine, cinchonine, cinchonidine, optochine, quinoline, ethylmorphine, thebaine, dibucaine, tropacocaine, betaeucaine, sod. cholate, sod. taurocholate, sod. desoxycholate, Irgapyrine, dibenamine, Priscol, jalapa resin, ethylalcohol, acetone. HgCl(2), phenol, thymol, alkylresorcinol, oil of sinapis, oil of chenopodium and ichthammol. On the other hand, salicylic acid, acetylsalicylic acid and cocaine·HCl, under acidic condition, rather suppressed spontaneous release of histamine. The rates of histamine release from dog skin shown by these drugs were found on the whole to be higher than those obtained by Kamimura (Folia pharmacol. japon., 53, 836, 1957) of our laboratory using guinea-pig lung tissues. On the basis of these findings, the significance of histamine release in relation to symptoms of the so-called drug allergy or in the known pharmacological actions of certain drugs were discussed. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 69 8 1957 エビエキスの催リンパ作用とその本態,特に二次的活性物質Adenine nucleotidesの遊離について 2035 2047 EN Yukio Kamimura Kenji Tasaka Katsumi Nishijima Masao Kume The lymphagogue effect of extract of lobster muscle, which is one of the first class of HEIDENHAIN'S lymphagogues, is essentially the action of adenine nucleotides liberated from the tissues by the injection of this extract. And also it is proven that these nucleotides are liberated in a larger quantity from organs in other parts of the body rather than from viscera. This conclusion is based on the following evidences. 1) Systemic-venous injection of the extract accelerate the outflow of a uncoagulable, highly proteinized lymph from the thoracic duct of dog, accompanied by a fall in arterial blood pressure and a rise in portal pressure. All these phenomena are also observable by the intravenous injection of adenosine triphosphate (ATP). 2) Repeated injections weaken the reactions to this extract (Tachyphylaxis). 3) Immediately after vascular administration of the extract adenine nucleotides including ATP are detected in blood plasma spectrophotometrically as well as paperchromatographically. Quantitatively the content in nucleotides is higher in the plasma from the femoral vein after systemic-venous injection than from the hepatic vein after portal injection. 4) No definite increase of histamine in blood can be recognized after systemic-venous injection of the extract. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 69 8 1957 Congo red静注の催リンパ作用とその機序について 2023 2034 EN Yukio Kamimura Kenji Tasaka Katsumi Nishijima Masao Kume Intravenous injection of 2% normal saline solution of Congo red in a dosage of 5 cc/kg in a dog resulted in marked increase of lymph flow from the thoracic duct over several hours and the lymph was apparently less coagulable after injection of the dye. In a majority of cases, arterial pressure showed only a slight fall by this injection, while portal pressure showed a marked elevation accompanied with an increase of liver volume. Such changes in the flow of lymph and circulation could not be reproduced by the injection of a saline solution isosmotic with the dye solution. Appearance of a fairly large amount of hemoglobin in the plasma and lymph after injection of the dye solution was observed by spectrophotometer. This hemolysis was completely protected by the previous injection of heparin into the vein before injection of the dye solution but the increased flow of lymph and elevation of portal pressure effected by the dye solution could not be modified. In a dog administered with heparin to remove the effect of hemolyis, injection of the dye solution was observed to cause increase in refractive index of the lymph and decrease of the same of the plasma. This is an indication that a large amount of plasma protein had filtrated into the lymph. After injection of the dye solution, histamine content in the lymph was practically unchanged. Antihistamines failed to suppress the increased flow of the lymph and elevation of portal pressure. In a non-heparinized dog, appearance of a substance or substances assumed to be adenine nucleotides was observed in the plasma by spectrophotometry and paper chromatography immediately after injection of the dye solution. It was concluded from these results that the increased flow of the thoracic lymph caused by Congo red is related to congestion of blood in the liver and other organs in the abdominal cavity, and further it was deduced that the cause common to these phenomena is not blood coagulation and liberation of toxic substances (including adenine nucleotides) from blood cells into plasma, which are considered to be the cause of acute dye shock, but is some kind of injuries to peripheral vessels, especially to vascular system in the abdominal organs. No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 46 2 1992 Changes in plasma human atrial natriuretic peptide (hANP) level in normal pregnancy and pregnancy induced hypertension. 83 86 EN Nobutsugu Oguni Yukiteru Mitsui Shigehito Kamimura Katsuto Eguchi Kaoru Sekiba Article 10.18926/AMO/32650 <p>We determined plasma human atrial natriuretic peptide (hANP) levels in normal pregnancy and pregnancy induced hypertension (PIH). The plasma hANP levels slightly decreased in the first trimester of normal pregnancy and tended to recover as pregnancy advanced, although these changes were slight. However, the plasma hANP level in puerperium was higher than that in the third trimester of normal pregnancy. The plasma hANP level in mild PIH was not significantly higher than that in the third trimester of normal pregnancy. In contrast, the plasma hANP level in three cases of severe PIH was approximately 200% higher than those in the normal third trimester and mild PIH.</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 45 3 1991 Localization and developmental change of indoleamine 2,3-dioxygenase activity in the human placenta. 135 139 EN Shigehito Kamimura Katsuto Eguchi Masaru Yonezawa Kaoru Sekiba Article 10.18926/AMO/32206 <p>Previously, we pointed out the importance of the kynurenine metabolism in fetuses and neonates. We examined localization and developmental change of indoleamine 2,3-dioxygenase activity in human placenta. The indoleamine 2,3-dioxygenase was found localized in syncytiotrophoblast in the placenta. The indoleamine 2,3-dioxygenase activity was not detected in placenta in the early stage of gestation. It was first detected at around 14 weeks of gestation, increased rapidly thereafter and was maintained at high levels till near term. The indoleamine 2,3-dioxygenase activity was significantly lower in placenta with retarded intrauterine development. These results suggest the importance of placental indoleamine 2,3-dioxygenase during fetal development.&#60;/P&#62;</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 45 2 1991 Tryptophan and its metabolite concentrations in human plasma and breast milk during the perinatal period. 101 106 EN Shigehito Kamimura Katsuto Eguchi Kaoru Sekiba Article 10.18926/AMO/32183 <p>Concentrations of tryptophan (free and protein bound) and its metabolites in plasma of maternal vein at delivery, umbilical vein, umbilical artery, neonatal vein and breast milk were determined by high performance liquid chromatography. The plasma levels of tryptophan and most of its metabolites in umbilical vein and artery were significantly higher than those in maternal vein. The concentration of total tryptophan in plasma of neonatal vein showed marked decrease at 24 h after birth in comparison with that at birth, but the total kynurenine concentration was not decreased in plasma of neonatal vein. The colostrum contained a high level of total tryptophan. There were high ratios of free to total tryptophan in colostrum, transitional and mature milk. In the blood, ratios of free to total of tryptophan and kynurenine were kept at constant level throughout the perinatal period.</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 15 5 1961 The identification of menstrual blood by means of paper electrophoresis, a medico-legal study 317 328 EN Osamu Kamimura Article 10.18926/AMO/31398 <p>Attempts were made to identify menstrual blood by means of paper electrophoresis with preparation of extracts of menstrual blood isolated under various conditions and mixed with human fibrin. Also similar analyses were conducted with blood aspirated from the median cubital vein of a woman during menstruation as well as from a man as the control, also with extracts of lochial blood from a woman after normal delivery, and of the blood obtained at arrtificial abortion. Animal fibrins (from rabbit, mouse, steer, and guinea pig) were also used to see the lytic action of the bloods. The following are the results of the present experiments. 1. The identification of menstrual blood by means of paper electrophoresis is a simple method in legal medicine and its electrophorogram is an excellent method to offer an evidence of proof for mentrual blood. 2. By this method it is possible to identify the menstrual bloodstain even after the lapse of time as much as 6 months. 3. It is possible to identify even putrefied menstrual bloodstain. 4. In the case where the material stained with menstrual blood is found in water, it is not possible to identify the menstrual blood by this method. 5. When the menstrual blood is heated at 60°C over 30 minutes, it becomes impossible to identify it by this method. 6. In the case of venous blood during menstruation fibrinolytic product can be detected only on the first day of menstruation, but since it appears only in trace, it is easy to differentiate it from menstrual blood. 7. As for lochial blood the fibrinolytic product can be detected only in the blood obtained on the first and second days of puerperium, but the amount being so slight that it can readily be distinguished from menstrual blood. 8. In the case of the blood obtained at artificial abortion fibrinolytic product appears just as much as in the case of menstrual blood, and thus it is impossible to differentiate it from mentrual blood by this method. 9. As for the use of human fibrin it is best to employ it while it isdresh, but the human fibrin up to 6 days old can be used. However, the older is the human fibrin the lesser the fibrinolytic product detectable. 10. In the case using animal fibrins mixed with the extract of menstrual blood some do produce fibrinolytic product in trace, but since there is a danger of also producing the fibrinolytic product-like substance in venous blood, it is advisable not to use animal fibrins.</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 13 3 1959 Ex­perimental study and practice on the detection of vegetative planktons in the bone marrow of the drowned dead body 259 268 EN Yoshio Mikami Mizuho Kanda Osamu Kamimura Masami Okuyama Article 10.18926/AMO/31233 <p>Experiment on the animals proved that in the case of the death by drowning planktons always immigrate into the bone marrow and some kinds of them can be detected in the bone marrow even after a long period of time, suggesting that the detection of these planktons in the bone marrow of the dead person will give the important clue for the determination of the cause of death by drowning. Actually applying this method in a decayed corpse, we could successfully show the cause of death is due to drowning in which the cause of death was long argued in the court.</p> No potential conflict of interest relevant to this article was reported.

Okayama University Medical School Acta Medica Okayama 0386-300X 29 4 1975 Effect of neocarzinostatin on hemolytic plaque-forming cell production in mice 327 328 EN Isao Takahashi Hiroshi Mikochi Junya Sakato Toshio Nakanishi Hironobu Toki Okinobu Kamimura Koichi Kitajima Brief Note 10.18926/AMO/30906 No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 73 10-12 1961 植物性プランクトン珪藻の検出による溺死の研究補遺 第2編 クロロホルム，エーテルおよびラボナールによる各麻酔時，アルコール酩酊時ならびにブロバリン，アドルムおよびバラミン各投与による催眠時において河中に投じ死亡させたウサギの死体臓器ならびに骨からの珪藻の検出について 759 769 EN Mizuho Kanda Masami Okuyama Osamu Kamimura Hisashi Hattori Hideo Takemaru Masumi Shiraishi The authors have experimented to detect the vegetative planktons (diatoms) from organs and bones of the rabbits that were drowned to death under light and deep narcosis with chloroform, ether and Ravonal, under light, middle and deep drunkenness, under hypnosis by administration with Brovarin, Adorm and Valamin. Results are following; 1) Planktons could be detected from organs and bones of rabbits that were drowned to death under narcosis with chloroform, ether and Ravonal, under few degrees of drunkenness with alcohol, under hypnosis by administration with Brovarin, Adorm and Valamin. 2) Planktons could be detected almost same number in the experiments using the rabbits under narcosis with ether and Rabonal and light narcosis with chloroform, but smaller numbers under deep narcosis with chloroform. 3) As become deeper the drunkenness, smaller numbers of planktons could be detected in the experiments used alcohol. 4) In the experiments used rabbits under hypnosis by administration with Brovarin, Adorm and Valamin, total number of planktons detected from the organs and bones of rabbits lesser in case of using Brovarin, Adorm and Valamin orderly. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 73 10-12 1961 植物性プランクトン珪藻の検出による溺死の研究補遺 第1編 頸動脈切傷による出血時,頭部打撲による脳震盪時および絞頸における終末呼吸時において河中に投じ死亡させたウサギの死体臓器ならびに骨から珪藻の検出について 749 758 EN Mizuho Kanda Masami Okuyama Osamu Kamimura Hisashi Hattori Hideo Takemaru Masumi Shiraishi The authors have experimented to detect the planktons from the drowned dead bodies under the special conditions. Rabbits were thrown into the ditch water after they were injured the carotid artery, during the terminal breathing by strangulation and concussion of the brain. It was tried to detect the planktons after the incinaration of organs and bones. Results are following; 1) Planktons could not be detected from organs and bones of rabbits that were put the ditch water into stomach with catheterization. 2) Planktons could be detected from the organs and bones of rabbits that were drowned to death after injured to the carotid artery, but longer the time after injured, lesser the plankton can be detected. 3) Excepting lung, there were no planktons in the organs and bones of the rabbits that were thrown into the river after death by bleeding. 4) From the organs and bones of the rabbits that were thrown into the river under standstill of therminal breathing by strangulation, several plankton could be detected though in small number. 5) Planktons could be detected from organs and bones of the rabbits that were drowned to death in the river under concussion of the brain. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 73 4-6 1961 植物性プランクトン珪藻の検出の1方法について 257 259 EN Mizuho Kanda Masami Okuyama Osamu Kamimura Hisashi Hattori Authors have had experiment to lessen the trouble on the detection of vegetative plankton under the microscope. Used indian ink. To make 5 and 10% diluted indian ink and to put it into sediment, it is very easily and clearly to contrast with the back ground. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 73 4-6 1961 腐敗が高度で死因の判定が困難であつた水死体の2鑑定例 251 256 EN Mizuho Kanda Osamu Kamimura Masami Okuyama Hisashi Hattori Authors have had occasions to perform autopsy on two bodies that were hardly to determine the cause of death for the high degree of putrefaction. One corpse. covered with flanket, was laid on ford and the other was discovered at the river side. In each instance, we could succeed the detection of vegetative plankton in the organs of general circulatory system. and then revealed that the cause of death were attributed to drowning. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 76 1-3 1964 人体脊髄運動ニウロンのH-reflex並に動作様式に関する研究 第2編 人体運動ニウロンの動作様式に関する筋電図学的研究 63 74 EN Masayuki Kamimura The study was performed by EMG. record of Parkinsonsism tremor and tremor-like movement of normal subject imitate to Parkinsonism tremor. H-reflex technique by electric stimulation on the posterior tibial nerve was combined. In grouping voltage of Parkinsonsism tremor as well as in voluntary tremor-like movement of normal subject, double discharge of the single NMU. was observed generally with a interval of 25 to 60 millisecond (ms.), and the double discharging unit was considered to be a kinetic NMU. H-reflex records in series by relatively long interval (low repetition) stimulation showed constant amplitude of H-reflex. But series by relatively short interval (high repetition) stimulation showed irregular amplitude, and by shorter than 30ms. interval stimulation H-reflex default at random. At some critical conditien e.g. 68ms. interval at gastrocnemius muscle, 95ms. at soleus mnscle the series of H-reflex showed two kind of amplitude., higher amplitude H-reflex and lower amplitude H-reflex appeared in each othor. Depending on the amplitude of repetitive M-reflex will not fluctuate even at short interval such as 20ms. as already described on other literatures, the auther would conclude that above mentioned fluctuation of H-reflex amplitude was caused by the character of the afterhyperpolarization of motoneuron. The paticular phenomen of amplitude fluctuation of H-reflex which appeared as higher amplitnde and lower amplitude in each other would be considered due to mixing existance of two kinds neuromuscular units those have shorter and longer hyperpolarization time, and those two kinds of units would adequate to kinetic and tonic neuromusclarunits. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 76 1-3 1964 人体脊髄運動ニウロンのH-reflex並に動作様式に関する研究 第1編 人体H-reflexにおける促進及び回復曲線に関する実験的研究ならびに理論的考察 47 62 EN Masayuki Kamimura Serial records of H-reflex of splnal motoneuron were obtained by the recording of action potential of soleus muscle, triggered by conditioning and testing electrical shocks on the posterior tibial nerve. The fascilitation and recovery process of the spinal motoneuron was studied by the serial records of the H-reflex. The clinical material was 30 cases including normal subjects, patients with pyramidal lesion, and patients in acute stage of cerebrel intervantion. In commonly, the H-reflex triggered by test shock (H) was not observed by the test shock which was given within 3 millisecond (ms') following conditioning shock, but start to appeared at 4ms appeared in peak amplitude (maximal deflection) at 6ms., and disappeared by 9 to 13ms. Later than 9 to 13ms., H-reflex falled in a silent period. However, the detail of the abovementioned characteristics of the H-reflex appeared in some difference, i.e. on the patient with pyramidal lesion H-reflex continued to appear until 18ms. with maximal deflection of 150% of the H-reflex, on the normal subject those were 10ms. and on the patient in acute stadium of cerebral surgical intervention those were 9ms. and 50% . The author intended to describe the complate disappearance of H-reflex within 3ms. following conditioning shock as the first silent period, and presumed that the first silent period would be the result of combination of refractory period of Group Ia fiber and inhibition of Group Ib fiber. The auther also would suppose the detailed characteristics of the H-reflex at the period of 4 to 10ms. following conditioning shock was caused by the afterhyperexcitability of the motoneuron by the conditioning shock as well as summation of the test shock, but the IPSP of Group Ib efferent impulses and influence of Renshow cell were other important mechanisms. The author intended to call abovementioned brocess the fascillitatry of human H-reflex. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 89 5-6 1977 急性白血病の化学療法に関する研究 第2編 Neocarzinostatinを中心にした多剤併用による寛解導入療法 701 711 EN Okinobu Kamimura In an attempt to improve the chemotherapy of acute leukemia in adults, the author has studied the induction therapy on 63 cases with acute leukemia with Neocarzinostatin (NCS; N) in combination with conventional antileukemic agents; Vincristine (V), Daunorubicin (D), Cytosine Arabinoside (A), 6-Mercaptopurine-Riboside (M) and Prednisolone (P). Eighteen cases with acute lymphocytic leukemia (ALL) was treated with protocol A (NVMP, NDMP, NAMP). Out of 45 cases with acute non-lymphocytic leukemia (ANLL), 25 was treated with protocol A and 20 treated with protocol B (NADP, NAVP), and obtained the following results. 1) Of all 63 cases 51 (81.6%) attained complete remission and 9 (14.3%) partial remission. 2) Complete remission was obtained in 15 cases (83.3%) out of 18 with ALL and 36 cases (80.0%) of 45 with ANLL; the latter cases with ANLL consisted of 18 of 25 cases (72.0%) treated with protocol A and 18 of 20 cases (90.0%) with protocol B. 3) Refractory varieties included cases with acute myelogenous leukemia who had passed through atypical hematological findings, cases with monocytic leukemia, cases with WBC count over 50,000 per μl or with nucleated cell count in bone marrow over 500,000 per μl before treatment and cases over 50 years old. 4) In this induction therapy with NCS in combination, antileukemic effects was remarkable, while suppression on normal hematopoiesis, especially on thrombocytes, was very slight. 5) As to the other side effects no noteworthy disturbances other than occasional nausea, anorexia and liver damage in protocol B were observed. From these results it can be concluded that NCS in combination is one of the most potent remission induction therapies in acute leukemia in adults. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 89 5-6 1977 急性白血病の化学療法に関する研究 第1編 Neocarzinostatin単独による寛解導入療法 693 699 EN Okinobu Kamimura Neocarzinostatin (NCS) is an antitumor antibiotic isolated from streptomyces carzinostaticus by Ishida et al. in Japan in 1957. The chemical structure of NCS is a single chain made of acidic polypeptide consisting of 112 amino acids. Its antitumor activities have been demonstrated in various experimental tumors. Its mode of action is considered to inhibit DNA synthesis at early stage of S phase and to block it at G(2) phase. The agent has been used clinically for solid tumors without remardable effects, although never been used in treating human leukemia. For the first time the author has given NCS alone to patients with acute leukemia in the dose ranging from 0.04-0.06 mg/kg/day for 4-5 days as one course of treatment. In case no sufficient remission was induced further courses of treatment were repeated after an internal of 4-10 days, and obtained the following results. 1) Of 18 cases 7 (38.9%) attained complete remission and 4 (22.2%) partial remission. 2) Complete remission was obtained even in the patients with refractory varieties of acute leukemia, i.e., acute promyelocytic leukemia, monocytic leukemia and leukemia in aged group. 3) No noteworthy side effects other than occasional nausea or anorexia were observed and suppression on normal hematopoiesis was very slight. From these results it can be concluded that NCS is one of the most potent antileukemic agents for inducing remission in acute leukemia. No potential conflict of interest relevant to this article was reported.

岡山医学会 Acta Medica Okayama 0030-1558 114 3 2003 インターフェロン治療後C型慢性肝炎患者の長期予後　―ウイルス学的著効例，生化学的著効例と無効例の比較― 275 281 EN Masanobu Miyake To evaluate the prognosis of the sustained biochemical responder after interferon (IFN) therapy, we retrospectively studied 252 chronic hepatitis C patients who were treated with IFN. Patients were divided into four groups: group A, sustained virological responders (n=84); group B,sustained biochemical but not virological responders (n=43); group C, incomplete responders (n=64); group D, non responders (n=61). The levels of several liver function tests were evaluated at the end of the observation period (4.2±1.6 years, mean±SD) compared with those at just before IFN therapy. The levels of cholinesterase, albumin, γ-globulin, zinc sufate turbidity test, platelet count and clearance rate of indocyanine green test improved in group A (p<0.05), became worse in group D (p<0.05) and did not change in group B. The incidence of hepatocellular carcinoma was significantly higher in group D than in group B (p<0.01);Kaplan-Meier method, log-rank test). The hazard ratio for hapatocarcinogenesis of the patients in group A and B was significantly lower than that in group C and D (hazard ratio: 0.27, range of 0.08-0.98; p=0.046) adjusted for age, gender, stage and total alcohol consumption. These results suggest that the progress of liver disease and liver carcinogenesis was more suppressed in sustained biochemical responders than in non reponders. No potential conflict of interest relevant to this article was reported.

岡山大学経済学会 Acta Medica Okayama 03863069 38 1 2006 ＜書評＞松尾展成，『日本＝ザクセン文化交流史研究』，大学教育出版，２００５年 99 105 EN Hideo Takechi Takehiko Seto Naoki Kamimura No potential conflict of interest relevant to this article was reported.

岡山大学教育学部附属教育実践総合センター Acta Medica Okayama 1346-3705 4 1 2004 中学生のVDT作業の視覚認知特性 81 89 EN Norio Hatagi Yuuko Kamimura Yohei Hinata Yumi Yamaguchi Haruhisa Yamaguchi 10.18926/11368 本研究は、学校教育における学齢に適した教育環境設定のための基礎的研究として、中学生を対象にVDT作業において重要な意味を持つ、見やすい適正な字体、文字の大きさが学齢によって違うことを検証し、大学生（大人）との比較により学齢発達段階ごとの見やすいと感じる画面条件の差異を考究するための実験を行った。すなわち中学生と大学生を被験者として、11.5pから24pの文字ポイント数で代表的字体である明朝体とゴシック体で漢字仮名交じり文を視読及び入力させる実験を行いその結果を統計分析した。その結果、中学生の視認特性は大学生と比べて至適文字サイズが大きいことまた中学生でも低学年ほど大きな文字を好むことがわかった。しかし、明朝体とゴシック体の文字視読嗜好においては中学生と大学生には同じような傾向があることなどの知見が得られた。 No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254 87 1 1998 Activities of Iron Oxidase and Hydrogen Sulfide: ferric Ion Oxidoreductase of Thiobacillus ferrooxidans isolated from natural environments 77 83 EN Tsuyoshi Sugio Isao Fujiwara Mari Hanase Kazuo Kamimura It has been reported that both iron oxidase and hydrogen sulfide: ferric ion oxidoreductase (SFORase) were involved in bacterial leaching of metal ions from sulfide ores, and the amount of Cu2+ solubilized from copper ore by iron-oxidizing bacterium differed from strain. The activities of iron oxidase SFORase of iron-oxidizing bacteria isolated from the natural environments were determined. Iron-oxidizing activity and SFORase activity of 200 strains ranged from 1.20-1.65γmol/mg/min and from 0.11-2.80 γmol/mg/min, respectively. The findings that a remarkable difference was observed in the levels of SFORase activity, but not in iron-oxidizing activity, suggest that SFORase, but not iron oxidase, is the enzyme that determines the bacterial leaching activity of this bacterium. No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254 88 1 1999 深海環境への微生物の適応機構に関する研究 121 130 EN Kazuo Kamimura The world's oceans cover 70% of the eath's surface,with about 3,800m of average depth.Altough　the deep-sea environment with its high pressure and low temperatures is too extreme for most terrestrial and marine surface microorganisms,many barotolerant and barophilic bacteria have been found inhabiting the deep-sea.It is exyremely important for barophilic or barotolerand deep-sea bacteria to maintain the physiological functions of cytoplasmic membrane,which serves many vital functions.The fluidity of this cytoplasimic membrane composed of phospholipids and poteins is essential for the physiological functions of cells.As higher hydorstatic pressure raise the melting point of lipids and cause phase transition of lipid under pressurs of up to 100MPa,barotolerant and barophilic bacrteria under high hydostatic pressure appear to regulate the composition of their membrane phospholipids. Therfore the characrization of cytoplasmic membrane under high pressure is indispensable to clarify the mecanisms of bacteria adaptation to the deep-sea enviroment.The effects of pressure and temperature acid compositon of barotolerant deep-sea bacteria were investigated.Deep-sea bacteria maintained their membrane fluidity by increasing the content of unique fatty acid in phospholipids under high hydrostatic pressure.Gene expression seems to be necessary for the synthesis of unique fatty under high hydrostatic pressure. No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254 91 1 2002 Mechanism of Oxidation of Reduce Sulfur Compounds by Sulfur-Grown Acidithiobacillus Caldus Strain GO-1 23 29 EN Kazuo Kamimura Ryoko Sawada Tsuyoshi Sugio The oxidation of reduced sulfur compounds was studied by using resting cells of sulfur-grown Acidithiobacillus caldus strain GO-1. The optimum pHs for the oxidation of thiosulfate, tetrathionate, sulfur, sulfite and sulfide were 2, 3, 3-6, 7 and 7, respectively. The highest oxidation rate was observed with sulfite. The oxidation rates of the reduced sulfur compounds were measured in the absence or presence of inhibitors and uncouplers. 2, 4-dinitrophenol (DNP) and carbonyl cyanide-m-chlorophenylhydrazone (CCCP) strongly inhibited the oxidations of sulfur and sulfite. N-Ethylmaleimide (NEM) strongly inhibited the oxidation of tetrathionate and sulfur. 2-heptyl-4-hydroxy-quinoline-N-oxide(HQNO) inhibited the oxidation of sulfur and sulfite. The results suggested that tetrathionate was oxidized in the periplasmic space, and sulfur and sulfite were oxidized in the cytoplasm. Pyridine ferrohemochromes prepared from the membrane of strain GO-1 cell revealed the involvement of cytochromes b and c. Ubiquinol oxidase activity was detected in strain GO-1 cell, but cytochrome c oxidase measured by using mammalian cytochrome c as an electron donor was not detected in the cell. On the basis of the results a model for the metabolism of the reduced sulfur compounds by At. caldus strain GO-1 was proposed. No potential conflict of interest relevant to this article was reported.

岡山大学農学部 Acta Medica Okayama 0474-0254 95 1 2006 Discrimination among the Three Acidithiobacillus Species, A. ferrooxidans, A. thiooxidans and A. caldus, Based on Restriction Fragment Length Polymorphism Analysis of the 16S-23S rDNA Intergenic Spacer Region 7 11 EN Satoshi Wakai Kentaro Yamamoto Tadayoshi Kanao Tsuyoshi Sugio Kazuo Kamimura The PCR-amplified 16S-23S rDNA intergenic spacer regions (ISRs) of Acidithiobacillus ferrooxidans, A. thiooxidans, and A. caldus strains were scquenced and evaluated for differentiation and identification of these bacteria. The total length of the 16S-23S ISRs of A. ferrooxidans and A. thiooxidans strains and A.caldus GO-1 were 441, 456, nd 379bp, respectively. Two genes. encoding tRNA and tRNA, and the box A-like sequences were highly conserved in the ISRs of all Acidithiobacillus species. The restriction fragment length polymorphism (RFLP) profiles of the PCR-amplified 16S-23S rDNA ISRs digested by HaeIII and AluI could clearly discriminate A. ferrooxidans from A. thiooxidans and A. caldus. The results indicated that RFLP analysis of the 16S-23S ISRs is an easy and rapid method for discrimination and identification of Acidithiobacillus species. No potential conflict of interest relevant to this article was reported.