start-ver=1.4
cd-journal=joma
no-vol=20
cd-vols=
no-issue=3
article-no=
start-page=e20220127
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=2023
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Rapid thawing of frozen bull spermatozoa by transient exposure to 70 °C improves the viability, motility and mitochondrial health
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Up to now, the definitive conclusion of the positive effects of rapid transient thawing at higher temperatures for shorter durations has not been obtained yet and is still under discussion due to some contradictory findings and limited assessment of post-thawed parameters. The purpose of the current study was to evaluate the effectiveness of rapid thawing in water at 70 °C by using various post-thawed parameters of frozen bull spermatozoa. Experiment 1, monitoring the change of temperature inside frozen bull straw thawed in water at different temperatures. Experiment 2, evaluation of various post-thawed characteristics of frozen bull spermatozoa thawed in water at different temperatures by using a computer-assisted sperm analysis, flow cytometry and immunocytochemistry. The time it took for the temperature inside the straw to warm up to 15 °C was nearly twice as faster when the straw was thawed in 70 °C water compared with 39 °C. Although there were differences among bulls, viability, motility, and mitochondrial membrane potential of spermatozoa thawed at 70 °C for 8 seconds and stabilized at 39 °C for 52 seconds were significantly higher than those of controls (thawed at 39 °C for 60 seconds) at 0 and 3 h after thawing. Just after thawing, however, there were no differences in acrosome integrity and distribution of phospholipase C zeta1, whereas mitochondrial reactive oxygen species production was significantly lower in spermatozoa thawed at 70 °C. From these results, we conclude that rapid thawing at 70 °C and then stabilization at 39 °C significantly improves viability, motility and mitochondrial health of bull spermatozoa rather than conventional thawing at 39 °C. The beneficial effect of rapid transient thawing could be due to shorter exposure to temperatures outside the physiological range, consequently maintaining mitochondrial health.
en-copyright=
kn-copyright=
en-aut-name=NguyenHai Thanh
en-aut-sei=Nguyen
en-aut-mei=Hai Thanh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=DoSon Quang
en-aut-sei=Do
en-aut-mei=Son Quang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=AthurupanaRukmali
en-aut-sei=Athurupana
en-aut-mei=Rukmali
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WakaiTakuya
en-aut-sei=Wakai
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=bull semen
kn-keyword=bull semen
en-keyword=cryopreservation process
kn-keyword=cryopreservation process
en-keyword=phospholipase C zeta1 (PLCZ1)
kn-keyword=phospholipase C zeta1 (PLCZ1)
en-keyword=temperature of thawing
kn-keyword=temperature of thawing
END
start-ver=1.4
cd-journal=joma
no-vol=42
cd-vols=
no-issue=3
article-no=
start-page=177
end-page=185
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2024
dt-pub=20240718
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Reduced fitness in losers of leg-biting male combat compared to uncontested males in Zophobas atratus
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Sexual dimorphism and male combat are observed in many species. Often, the outcome of male combat affects the outcome of subsequent combats, mating success, number of sperm, and fitness of the male’s offspring. Also, the quantity and quality of sperm may be regulated by winning or losing, depending on species ecology and mating system. However, very few studies have experimentally examined the influence of fight outcomes on male offspring fitness. We studied male combat in the giant mealworm (Zophobas atratus) in which males bite each other’s hind legs. We hypothesized that subsequent fitness could differ between winners and losers in the escalated male combat of this species. We measured several fitness traits including the number of eggs laid by mated females, and the number of hatches sired by uncontested males, winners, and losers in escalated and non-escalated combat, and compared the fitness of each winner and loser to that of an uncontested male. We also measured mating duration. The numbers of eggs and the percentages of hatched eggs of losers in the escalated combat were significantly reduced compared to that of the uncontested males. This reduction may be due to injuries from escalated leg- biting fights and a result of the sperm amount of the uncontested males being greater than that of the loser males.
en-copyright=
kn-copyright=
en-aut-name=MatsuuraTeruhisa
en-aut-sei=Matsuura
en-aut-mei=Teruhisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MiyatakeTakahisa
en-aut-sei=Miyatake
en-aut-mei=Takahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
affil-num=1
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=
affil-num=2
en-affil=Graduate School of Environmental, Life, Natural Science and Technology, Okayama University
kn-affil=
en-keyword=Beetle
kn-keyword=Beetle
en-keyword=Offspring fitness
kn-keyword=Offspring fitness
en-keyword=Male combat
kn-keyword=Male combat
en-keyword=Hind leg
kn-keyword=Hind leg
en-keyword=Weapon
kn-keyword=Weapon
END
start-ver=1.4
cd-journal=joma
no-vol=210
cd-vols=
no-issue=
article-no=
start-page=154
end-page=161
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2023
dt-pub=20231015
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Negative correlations of mitochondrial DNA copy number in commercial frozen bull spermatozoa with the motility parameters after thawing
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The purpose of the current study was to investigate the relationship between mitochondrial content of commercial frozen-thawed bull spermatozoa and motility. Firstly, mitochondrial DNA copy number per spermatozoon (MDCN), mitochondrial content (MC), the percentage of spermatozoa with high mitochondrial membrane potential (HMMP), intracellular reactive oxygen species (ROS) and motility parameters of frozen-thawed spermatozoa derived from five bulls were determined by using qPCR, flow cytometry and CASA, respectively, and analyzed the relationships. Results showed that all parameters examined, including MDCN, MC, HMMP, ROS and motility indicators, significantly differed among frozen spermatozoa from different bulls. Both MDCN and MC were negatively correlated with HMMP and motility indicators, but positively with ROS, of course, whereas there was a highly positive relationship between MDCN and MC. Secondly, when MDCN and MC were examined in frozen spermatozoa prepared at different points in the lives of four bulls, those did not correlate overall throughout their lives (1.3?14.3 years old), but did correlate significantly in two sires. From these results, we conclude that MDCN and MC of frozen spermatozoa differ among sires, and are negatively correlated with HMMP and sperm motility parameters, probably due to mitochondrial oxidative stress resulted in the presence of ROS, demonstrating that these appear to be useful markers to assess sires’ spermatozoa. It should be noted that the MDCN and MC of bull spermatozoa may not vary overall with the age of the sire, whereas those changes with age in some individuals and may affect sperm motility.
en-copyright=
kn-copyright=
en-aut-name=NguyenHai Thanh
en-aut-sei=Nguyen
en-aut-mei=Hai Thanh
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=DoSon Quang
en-aut-sei=Do
en-aut-mei=Son Quang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KobayashiHiroshi
en-aut-sei=Kobayashi
en-aut-mei=Hiroshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=WakaiTakuya
en-aut-sei=Wakai
en-aut-mei=Takuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=3
en-affil=Okayama Prefectural Center for Animal Husbandry and Research
kn-affil=
affil-num=4
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=5
en-affil=Department of Animal Science, Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=Spermatozoa
kn-keyword=Spermatozoa
en-keyword=Bulls
kn-keyword=Bulls
en-keyword=Mitochondrial content
kn-keyword=Mitochondrial content
en-keyword=Motility
kn-keyword=Motility
en-keyword=Frozen semen
kn-keyword=Frozen semen
END
start-ver=1.4
cd-journal=joma
no-vol=13
cd-vols=
no-issue=2
article-no=
start-page=285
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2022
dt-pub=20220131
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Dkk3/REIC Deficiency Impairs Spermiation, Sperm Fibrous Sheath Integrity and the Sperm Motility of Mice
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The role of Dickkopf-3 (Dkk3)/REIC (The Reduced Expression in Immortalized Cells), a Wnt-signaling inhibitor, in male reproductive physiology remains unknown thus far. To explore the functional details of Dkk3/REIC in the male reproductive process, we studied the Dkk3/REIC knock-out (KO) mouse model. By examining testicular sections and investigating the sperm characteristics (count, vitality and motility) and ultrastructure, we compared the reproductive features between Dkk3/REIC-KO and wild-type (WT) male mice. To further explore the underlying molecular mechanism, we performed RNA sequencing (RNA-seq) analysis of testicular tissues. Our results showed that spermiation failure existed in seminiferous tubules of Dkk3/REIC-KO mice, and sperm from Dkk3/REIC-KO mice exhibited inferior motility (44.09 +/- 8.12% vs. 23.26 +/- 10.02%, p < 0.01). The Ultrastructure examination revealed defects in the sperm fibrous sheath of KO mice. Although the average count of Dkk3/REIC-KO epididymal sperm was less than that of the wild-types (9.30 +/- 0.69 vs. 8.27 +/- 0.87, x10(6)), neither the gap (p > 0.05) nor the difference in the sperm vitality rate (72.83 +/- 1.55% vs. 72.50 +/- 0.71%, p > 0.05) were statistically significant. The RNA-seq and GO (Gene Oncology) enrichment results indicated that the differential genes were significantly enriched in the GO terms of cytoskeleton function, cAMP signaling and calcium ion binding. Collectively, our research demonstrates that Dkk3/REIC is involved in the process of spermiation, fibrous sheath integrity maintenance and sperm motility of mice.
en-copyright=
kn-copyright=
en-aut-name=XueRuizhi
en-aut-sei=Xue
en-aut-mei=Ruizhi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=LinWenfeng
en-aut-sei=Lin
en-aut-mei=Wenfeng
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=FujitaHirofumi
en-aut-sei=Fujita
en-aut-mei=Hirofumi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=SunJingkai
en-aut-sei=Sun
en-aut-mei=Jingkai
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=KinoshitaRie
en-aut-sei=Kinoshita
en-aut-mei=Rie
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=OchiaiKazuhiko
en-aut-sei=Ochiai
en-aut-mei=Kazuhiko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=FutamiJunichiro
en-aut-sei=Futami
en-aut-mei=Junichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=WatanabeMasami
en-aut-sei=Watanabe
en-aut-mei=Masami
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=OhuchiHideyo
en-aut-sei=Ohuchi
en-aut-mei=Hideyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
en-aut-name=SakaguchiMasakiyo
en-aut-sei=Sakaguchi
en-aut-mei=Masakiyo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=10
ORCID=
en-aut-name=TangZhengyan
en-aut-sei=Tang
en-aut-mei=Zhengyan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=11
ORCID=
en-aut-name=HuangPeng
en-aut-sei=Huang
en-aut-mei=Peng
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=12
ORCID=
en-aut-name=NasuYasutomo
en-aut-sei=Nasu
en-aut-mei=Yasutomo
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=13
ORCID=
en-aut-name=KumonHiromi
en-aut-sei=Kumon
en-aut-mei=Hiromi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=14
ORCID=
affil-num=1
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=5
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Laboratory of Veterinary Hygiene, Nippon Veterinary and Life Science University
kn-affil=
affil-num=7
en-affil=Department of Interdisciplinary Science and Engineering in Health Systems, Okayama University
kn-affil=
affil-num=8
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=9
en-affil=Department of Cytology and Histology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=10
en-affil=Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=11
en-affil=Department of Urology, Xiangya Hospital, Central South University
kn-affil=
affil-num=12
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=13
en-affil=Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=14
en-affil=Innovation Center Okayama for Nanobio-Targeted Therapy, Okayama University
kn-affil=
en-keyword=Dkk3/REIC
kn-keyword=Dkk3/REIC
en-keyword=fibrous sheath
kn-keyword=fibrous sheath
en-keyword=knock-out
kn-keyword=knock-out
en-keyword=RNA-seq
kn-keyword=RNA-seq
en-keyword=spermiation
kn-keyword=spermiation
en-keyword=sperm motility
kn-keyword=sperm motility
END
start-ver=1.4
cd-journal=joma
no-vol=26
cd-vols=
no-issue=
article-no=
start-page=1196
end-page=1202
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2021
dt-pub=202156
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Otorhinolaryngologists/head and neck surgeons’ knowledge, attitudes, and practices regarding fertility preservation in young cancer patients treated with chemotherapy: an anonymous questionnaire survey
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Background: It is well known that chemotherapy for adolescent and young adult (AYA) patients with cancer can reduce fertility regardless of the regimen. A decline in fertility greatly affects the quality of life of cancer survivors in the AYA age group; however, few patients are thought to be receiving fertility preservation measures.
Methods: A questionnaire survey was conducted to assess the current understanding and consideration of fertility among otorhinolaryngologists/head and neck surgeons who treat AYA patients with cancer, and to inform them of the guidelines for fertility preservation. A total of 275 otorhinolaryngologists/head and neck surgeons working at our hospital in Ehime, Japan, six neighboring universities, and their affiliated facilities were included in this study. The questionnaire was mailed and requested to be returned by fax. Twenty questions were asked about respondents’ years of experience as physicians, specialties, experience in medical care and chemotherapy for AYA patients with cancer, and knowledge and experience in fertility reduction measures.
Results: Although 58.7% of the physicians were aware that cryopreservation of eggs and sperm prior to chemotherapy was recommended, only 7 out of 40 physicians (17.5%) had referred AYA patients with cancer to an appropriate medical facility (department) after obtaining informed consent for chemotherapy.
Conclusions: Although fertility preservation has been discussed at professional conferences and seminars, consideration and actions in the field of otorhinolaryngology/head and neck surgery have not been sufficient. We hope that the results of this survey will help raise awareness of fertility preservation.
en-copyright=
kn-copyright=
en-aut-name=AkisadaNaoki
en-aut-sei=Akisada
en-aut-mei=Naoki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MondenNobuya
en-aut-sei=Monden
en-aut-mei=Nobuya
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KishinoTakehito
en-aut-sei=Kishino
en-aut-mei=Takehito
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=AoiJiro
en-aut-sei=Aoi
en-aut-mei=Jiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=HayashiYuji
en-aut-sei=Hayashi
en-aut-mei=Yuji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=TakahashiSaori
en-aut-sei=Takahashi
en-aut-mei=Saori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=NakamuraMasataka
en-aut-sei=Nakamura
en-aut-mei=Masataka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=IshiharaHisashi
en-aut-sei=Ishihara
en-aut-mei=Hisashi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
en-aut-name=NishizakiKazunori
en-aut-sei=Nishizaki
en-aut-mei=Kazunori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=9
ORCID=
affil-num=1
en-affil=Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=3
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=4
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=5
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=6
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=7
en-affil=Department of Head and Neck Surgery, National Hospital Organization Shikoku Cancer Center
kn-affil=
affil-num=8
en-affil=Department of Otolaryngology, Japanese Red Cross Okayama Hospital
kn-affil=
affil-num=9
en-affil=Department of Otolaryngology-Head and Neck Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=fertility preservation
kn-keyword=fertility preservation
en-keyword=otorhinolaryngology
kn-keyword=otorhinolaryngology
en-keyword=head and neck cancer
kn-keyword=head and neck cancer
en-keyword=adolescent and young adult generation
kn-keyword=adolescent and young adult generation
en-keyword=guidelines
kn-keyword=guidelines
en-keyword=Japan
kn-keyword=Japan
END
start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=6
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2020
dt-pub=202002
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Promising New Anti-Cancer Strategy: Iron Chelators Targeting CSCs
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Iron is a trace but vital element in the human body and is necessary for a multitude of crucial processes in life. However, iron overload is known to induce carcinogenesis via oxidative stress. Cancer cells require large amounts of iron for their rapid division and cell growth. Iron was recently found to play a role in cancer stem cells (CSCs); it maintains stemness during development. Iron also plays an important role in stemness by moderating reactive oxygen species. Thus, iron metabolism in CSCs is a promising therapeutic target. In this review, we summarize the roles of iron in cancer cells and CSCs. We also summarize anti-cancer therapeutic studies with iron chelators and describe our expectation of a new therapeutic strategy for CSCs on the basis of our findings.
en-copyright=
kn-copyright=
en-aut-name=ChenYuehua
en-aut-sei=Chen
en-aut-mei=Yuehua
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=OharaToshiaki
en-aut-sei=Ohara
en-aut-mei=Toshiaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=XingBoyi
en-aut-sei=Xing
en-aut-mei=Boyi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=QiJiping
en-aut-sei=Qi
en-aut-mei=Jiping
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NomaKazuhiro
en-aut-sei=Noma
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=MatsukawaAkihiro
en-aut-sei=Matsukawa
en-aut-mei=Akihiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=3
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=4
en-affil=Department of Pathology, the First Affiliated Hospital of Harbin Medical University
kn-affil=
affil-num=5
en-affil=Department of Gastroenterological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=6
en-affil=Department of Pathology and Experimental Medicine, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=cancer stem cell
kn-keyword=cancer stem cell
en-keyword=stemness
kn-keyword=stemness
en-keyword=iron
kn-keyword=iron
en-keyword=chelation
kn-keyword=chelation
en-keyword=chemotherapy
kn-keyword=chemotherapy
END
start-ver=1.4
cd-journal=joma
no-vol=30
cd-vols=
no-issue=6
article-no=
start-page=1522
end-page=1529
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=20190705
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Artificial selection on walking distance suggests a mobility-sperm competitiveness trade-off
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= Securing matings is a key determinant of fitness, and in many species, males are the sex that engages in mate searching. Searching for mates is often associated with increased mobility. This elevated investment in movement is predicted to trade-off with sperm competitiveness, but few studies have directly tested whether this trade-off occurs. Here, we assessed whether artificial selection on mobility affected sperm competitiveness and mating behavior, and if increased mobility was due to increased leg length in red flour beetles (Tribolium castaneum). We found that, in general, males selected for decreased mobility copulated for longer, stimulated females more during mating, and tended to be better sperm competitors. Surprisingly, they also had longer legs. However, how well males performed in sperm competition depended on females. Males with reduced mobility always copulated for longer than males with high mobility, but this only translated into greater fertilization success in females from control populations and not the selection populations (i.e. treatment females). These results are consistent with a mate-searching/mating-duration trade-off and broadly support a trade-off between mobility and sperm competitiveness.
en-copyright=
kn-copyright=
en-aut-name=MatsumuraKentarou
en-aut-sei=Matsumura
en-aut-mei=Kentarou
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=ArcherC Ruth
en-aut-sei=Archer
en-aut-mei=C Ruth
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=Hosken David J
en-aut-sei=Hosken
en-aut-mei= David J
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MiyatakeTakahisa
en-aut-sei=Miyatake
en-aut-mei=Takahisa
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
affil-num=2
en-affil=Centre for Ecology & Conservation, University of Exeter
kn-affil=
affil-num=3
en-affil=Centre for Ecology & Conservation, University of Exeter
kn-affil=
affil-num=4
en-affil=Graduate School of Environmental and Life Science, Okayama University
kn-affil=
en-keyword=leg length
kn-keyword=leg length
en-keyword=mate searching
kn-keyword=mate searching
en-keyword=morphology
kn-keyword=morphology
en-keyword=sperm competition,
kn-keyword=sperm competition,
en-keyword=trade-off
kn-keyword=trade-off
en-keyword=Tribolium castaneum
kn-keyword=Tribolium castaneum
en-keyword=walking
kn-keyword=walking
END
start-ver=1.4
cd-journal=joma
no-vol=73
cd-vols=
no-issue=3
article-no=
start-page=213
end-page=221
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2019
dt-pub=201906
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Spiral Trajectory Modulation of Rheotaxic Motile Human Sperm in Cylindrical Microfluidic Channels of Different Inner Diameters
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract= We investigated the relationship between human sperm rheotaxis and motile sperm trajectories by using poly-(dimethylsiloxane) (PDMS)-based cylindrical microfluidic channels with inner diameters of 100 μm, 50 μm, and 70 μm, which corresponded to the inner diameter of the human isthmus, the length of a sperm and a diameter intermediate between the two, respectively. We counted the number of rheotaxic sperm and sperm with spiral motion. We also analyzed motile sperm trajectories. As the cylindrical channel diameter was decreased, the percentage of sperm cells exhibiting rheotaxis, the percentage of sperm cells exhibiting spiral motion, the frequency-to-diameter ratio of the sperm cells’ spiral trajectories, and the surface area of the microfluidic channel increased, while the flagellar motion at the channel wall decreased. The percentage of sperm exhibiting a spiral trajectory and the frequency-to-diameter ratio of the sperm cells’ spiral trajectories were thus affected by the channel diameter. Our findings suggest that the oviduct structure affects the swimming properties of sperm cells, guiding them from the uterus to the ampulla for egg fertilization. These results could contribute to the development of motile sperm-sorting microfluidic devices for assisted reproductive technologies.
en-copyright=
kn-copyright=
en-aut-name=NishinaSaori
en-aut-sei=Nishina
en-aut-mei=Saori
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
affil-num=1
en-affil=Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
affil-num=2
en-affil=Department of Biomedical Engineering, Faculty of Engineering, Okayama University of Science
kn-affil=
affil-num=3
en-affil=Cardiovascular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
kn-affil=
en-keyword=sperm motility
kn-keyword=sperm motility
en-keyword=trajectory
kn-keyword=trajectory
en-keyword=microfluidic channel
kn-keyword=microfluidic channel
en-keyword=rheotaxis
kn-keyword=rheotaxis
en-keyword=oviduct structure
kn-keyword=oviduct structure
END
start-ver=1.4
cd-journal=joma
no-vol=68
cd-vols=
no-issue=4
article-no=
start-page=207
end-page=218
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2014
dt-pub=201408
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=A Questionnaire Study on Attitudes toward Birth and Child-rearing of University Students in Japan, China,and South Korea
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This study examines the attitudes of young Japanese, Chinese, and South Koreans toward birth and child-rearing. The survey targeted four-year university students (n＝1,668) who responded to an anonymous survey using self-report questionnaires between December 2012 and April 2013. The collection rates were 72.5%, 94.7%, and 96.5% for the Japanese, Chinese, and South Korean students, respectively. Correlations among the respondents? attributes, medical and scientific literacy levels, and views of preferred qualities of children were analyzed using chi-square test, supplemented by residual analysis (significance level set at p＜0.05). Participants were asked whether they were willing to use the following methods for obtaining preferred qualities in their children:(1) choosing a spouse (43.2%, 72.6%, and 85.1% of the Japanese, Chinese, and South Koreans, respectively, agreed);(2) using a sperm bank (cryobank) (5.8%, 60.1%, and 81.7% of the Japanese, Chines, and South Koreans, respectively, agreed);and (3) using an egg cell bank (ova bank or cryobank) (5.3%, 47.2%, and 70.3% of the Japanese, Chinese, and South Koreans, respectively, agreed). The proportion of affirmative responses (indicating “eugenic inclination”) to these statements was significantly higher among the Chinese and South Korean participants than their Japanese counterparts (p＜0.001). Significant differences were also found in the attitudes of the 3 groups toward methods for obtaining the preferred qualities for their children:prenatal diagnosis, pre-implantation diagnosis, the environment during pregnancy, and child-rearing.
en-copyright=
kn-copyright=
en-aut-name=YuLiling
en-aut-sei=Yu
en-aut-mei=Liling
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=KatoYutaka
en-aut-sei=Kato
en-aut-mei=Yutaka
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=ShishidoKeisuke
en-aut-sei=Shishido
en-aut-mei=Keisuke
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=DoiHideko
en-aut-sei=Doi
en-aut-mei=Hideko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=JinHaeng mi
en-aut-sei=Jin
en-aut-mei=Haeng mi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=WangJin gang
en-aut-sei=Wang
en-aut-mei=Jin gang
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=IkezawaJunko
en-aut-sei=Ikezawa
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
en-aut-name=AwayaTsuyoshi
en-aut-sei=Awaya
en-aut-mei=Tsuyoshi
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=8
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Bioethics, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=2
en-affil=
kn-affil=Japan Society for the Promotion of Science, Nagoya University of Economics
affil-num=3
en-affil=
kn-affil=Faculty of Law, Nagoya University of Economics
affil-num=4
en-affil=
kn-affil=Department of Nursing, Niimi College
affil-num=5
en-affil=
kn-affil=Graduate School of Sport Science, Kyonggi University
affil-num=6
en-affil=
kn-affil=Department of Minority Languages and Literatures, Minzu University
affil-num=7
en-affil=
kn-affil=Department of Bioethics, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
affil-num=8
en-affil=
kn-affil=Department of Bioethics, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
en-keyword=prenatal diagnosis
kn-keyword=prenatal diagnosis
en-keyword=pre-implantation diagnosis
kn-keyword=pre-implantation diagnosis
en-keyword=delivery and child-rearing
kn-keyword=delivery and child-rearing
en-keyword=eugenics
kn-keyword=eugenics
en-keyword=healthy birth and child-rearing
kn-keyword=healthy birth and child-rearing
END
start-ver=1.4
cd-journal=joma
no-vol=74
cd-vols=
no-issue=5
article-no=
start-page=863
end-page=870
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=20100915
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Application of a microfluidic sperm sorter to the in-vitro fertilization of porcine oocytes reduced the incidence of polyspermic penetration
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The objective of this study was to use a microfluidic sperm sorter (MFSS), designed to isolate motile human spermatozoa with laminar flows (no centrifugation), for porcine IVF. Boar spermatozoa were diluted at 1 x 10(8) with a diluent containing 20% seminal fluid and flowed with modified TCM-199 (mM199, with 5 mM caffeine) to introduce motile sperm into the exit chamber for IVF. In Experiment 1, after flowing for 5 min, sperm concentration varied significantly among specific sites within the MFSS collecting chamber (range, 0.8 +/- 0.5 x 10(4) to 575.0 +/- 56.3 x 10(4) cells/mL; mean +/- SEM). In Experiment 2, when porcine IVM oocytes were placed at three locations in the MFSS exit chamber (where only motile spermatozoa accumulated) and subsequently cultured in caffeine-free mM199 for 8 h, sperm penetration rate was not significantly different among places (86.1 +/- 10.5 to 100%), but the monospermic penetration rate was lower (P < 0.05) in oocytes 3.5 mm from the exit position (12.5 +/- 4.8%) than those at 7.5 mm (53.1 +/- 6.0%) or further (41.9 +/- 2.8%) from the exit. In Experiment 3, the normal fertilization index (ratio of monospermic oocytes to number of oocytes examined) 8 h after insemination was higher (P < 0.05) in the MFSS-IVF system (0.375 +/- 0.040) than both standard IVF and transient IVF (0.222 +/- 0.028 and 0.189 +/- 0.027, respectively, with co-culture for 8 h and for 5 min). Developmental competence of fertilized oocytes (blastocyst formation) was higher (P < 0.05) in the MFSS-IVF system (40.9 +/- 2.3%) than in either standard or transient IVF (22.6 +/- 1.4 and 33.7 +/- 3.5%). In conclusion, brief co-culture of porcine oocytes with spermatozoa gradually accumulated in the MFSS chamber improved the efficiency of producing monospermic fertilized embryos and blastocysts. Furthermore, efficiencies were significantly affected by oocyte location within the chamber.
en-copyright=
kn-copyright=
en-aut-name=SanoHikaru
en-aut-sei=Sano
en-aut-mei=Hikaru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Animal Science, Graduate school of Natural Science and Technology, Okayama University
affil-num=2
en-affil=
kn-affil=Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
affil-num=3
en-affil=
kn-affil=Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
affil-num=4
en-affil=
kn-affil=Department of Animal Science, Graduate school of Natural Science and Technology, Okayama University
en-keyword=Oocytes
kn-keyword=Oocytes
en-keyword=Polyspermy
kn-keyword=Polyspermy
en-keyword=IVF
kn-keyword=IVF
en-keyword=Sperm sorter
kn-keyword=Sperm sorter
en-keyword=Pig
kn-keyword=Pig
END
start-ver=1.4
cd-journal=joma
no-vol=56
cd-vols=
no-issue=5
article-no=
start-page=552
end-page=557
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2010
dt-pub=201010
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=In-vitro Culture with a Tilting Device in Chemically Defined Media During Meiotic Maturation and Early Development Improves the Quality of Blastocysts Derived from In-vitro Matured and Fertilized Porcine Oocytes
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Under physiological conditions, mammalian oocytes and embryos appear to be stimulated not only chemically but also mechanically, such as by compression, shear stress and/or friction force in the follicle and female reproductive tract. The present study was undertaken to examine the effects of kinetic culture with a tilting device in chemically defined media during in vitro maturation (IVM) of porcine oocytes and in vitro culture (IVC) following in vitro fertilization (IVF) on the early developmental competence and quality of blastocysts. After culture in a chemically defined IVM medium, modified porcine oocyte medium (mPOM) containing gonadotropins and dibutyryl cAMP for 20 h, the mean diameter of the cumulus-oocyte complexes (COCs) was larger in the tilting culture than in the static controls, whereas the diameter of the oocytes did not differ. When culture of the COCs was continued additionally in a fresh medium without gonadotropins and dibutyryl cAMP for 24 h, the incidences of oocytes completing GVBD and developing to the metaphase-II stage did not differ between the tilting and static culture systems. Furthermore, the sperm penetration after IVF and developmental competence of the oocytes to the blastocyst stage were not different between the tilting and static systems during IVM and IVC. However, tilting culture during both IVM and IVC had a significant positive effect on the number of cells per blastocyst (P<0.05). These observations indicate that tilting culture during IVM and IVC in chemically defined media improves the quality of blastocyst, as determined by the number of cells per blastocyst, without any effects on penetrability and developmental competence.
en-copyright=
kn-copyright=
en-aut-name=KoikeTakayuki
en-aut-sei=Koike
en-aut-mei=Takayuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=FunahashiHiroaki
en-aut-sei=Funahashi
en-aut-mei=Hiroaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
affil-num=1
en-affil=
kn-affil=Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University
affil-num=2
en-affil=
kn-affil=Research Core for Interdisciplinary Sciences, Okayama University
affil-num=3
en-affil=
kn-affil=Department of Cardiovascular Physiology, Graduate school of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
affil-num=4
en-affil=
kn-affil=Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University
en-keyword=Inclining device
kn-keyword=Inclining device
en-keyword=In vitro culture
kn-keyword=In vitro culture
en-keyword=In vitro fertilization
kn-keyword=In vitro fertilization
en-keyword=Oocytes
kn-keyword=Oocytes
en-keyword=Pig
kn-keyword=Pig
END
start-ver=1.4
cd-journal=joma
no-vol=11
cd-vols=
no-issue=1
article-no=
start-page=25
end-page=33
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2009
dt-pub=200902
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Application of a numerical simulation to improve the separation efficiency of a sperm sorter
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=This paper describes a study in which numerical simulations were applied to improve the separation efficiency of a microfluidic-based sperm sorter. Initially, the motion of 31 sperm were modeled as a sinusoidal wave. The modeled sperm were expected to move while vibrating in the fluid within the microchannel. In this analysis, the number of sperm extracted at the outlet channel and the rate of movement of the highly motile sperm were obtained for a wide range of flow velocities within the microchannel. By varying the channel height, and the width and the position of the sperm-inlet channel, we confirmed that the separation efficiency was highly dependent on the fluid velocity within the channel. These results will be valuable for improving the device configuration, and might help to realize further improvements in efficiency in the future.
en-copyright=
kn-copyright=
en-aut-name=HyakutakeToru
en-aut-sei=Hyakutake
en-aut-mei=Toru
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=HashimotoYuki
en-aut-sei=Hashimoto
en-aut-mei=Yuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=YanaseShinichiro
en-aut-sei=Yanase
en-aut-mei=Shinichiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=MatsuuraKoji
en-aut-sei=Matsuura
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NaruseKeiji
en-aut-sei=Naruse
en-aut-mei=Keiji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
affil-num=1
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
affil-num=2
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
affil-num=3
en-affil=
kn-affil=Graduate School of Natural Science and Technology, Okayama University
affil-num=4
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
affil-num=5
en-affil=
kn-affil=Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
en-keyword=Human reproduction
kn-keyword=Human reproduction
en-keyword=Microfluid
kn-keyword=Microfluid
en-keyword=Numerical simulation
kn-keyword=Numerical simulation
en-keyword=Separation efficiency
kn-keyword=Separation efficiency
en-keyword=Sperm sorter
kn-keyword=Sperm sorter
END
start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=4
article-no=
start-page=355
end-page=366
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020725
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Morphological features of lipid droplet transition during porcine oocyte fertilisation and early embryonic development to blastocyst in vivo and in vitro
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Lipid content in mammalian oocytes or embryos differs among species, with bovine and porcine oocytes and embryos showing large cytoplasmic droplets. These droplets are considered to play important roles in energy metabolism during oocyte maturation, fertilisation and early embryonic development, and also in the freezing ability of oocytes or embryos; however, their detailed distribution or function is not well understood. In the present study, changes in the distribution and morphology of porcine lipid droplets during in vivo and in vitro fertilisation, in contrast to parthenogenetic oocyte activation, as well as during their development to blastocyst stage, were evaluated by transmission electron microscopy (TEM). The analysis of semi-thin and ultra-thin sections by TEM showed conspicuous, large, electron-dense lipid droplets, sometimes associated with mitochondrial aggregates in the oocytes, irrespective of whether the oocytes had been matured in vivo or in vitro. Immediately after sperm penetration, the electron density of the lipid droplets was lost in both the in vivo and in vitro oocytes, the reduction being most evident in the oocytes developed in vitro. Density was restored in the pronculear oocytes, fully in the in vivo specimens but only partially in the in vitro ones. The number and size of the droplets seemed, however, to have decreased. At 2- to 4-cell and blastocyst stages, the features of the lipid droplets were almost the same as those of pronuclear oocytes, showing a homogeneous or saturated density in the in vivo embryos but a marbled or partially saturated appearance in the in vitro embryos. In vitro matured oocytes undergoing parthenogenesis had lipid droplets that resembled those of fertilised oocytes until the pronuclear stage. Overall, results indicate variations in both the morphology and amount of cytoplasmic lipid droplets during porcine oocyte maturation, fertilisation and early embryo development as well as differences between in vivo and in vitro development, suggesting both different energy status during preimplantation development in pigs and substantial differences between in vitro and in vivo development.
en-copyright=
kn-copyright=
en-aut-name=KikuchiKazuhiro
en-aut-sei=Kikuchi
en-aut-mei=Kazuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=EkwallHans
en-aut-sei=Ekwall
en-aut-mei=Hans
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=TienthaiPaisan
en-aut-sei=Tienthai
en-aut-mei=Paisan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=KawaiYasuhiro
en-aut-sei=Kawai
en-aut-mei=Yasuhiro
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=NoguchiJunko
en-aut-sei=Noguchi
en-aut-mei=Junko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=KanekoHiroyuki
en-aut-sei=Kaneko
en-aut-mei=Hiroyuki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
en-aut-name=Rpdriguez-MartinezHeriberto
en-aut-sei=Rpdriguez-Martinez
en-aut-mei=Heriberto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=7
ORCID=
affil-num=1
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=2
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=3
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=4
en-affil=
kn-affil=Okayama University
affil-num=5
en-affil=
kn-affil=National Institute of Agrobiological Sciences
affil-num=6
en-affil=
kn-affil=National Institute of Agrobiological Sciences
affil-num=7
en-affil=
kn-affil=Swedish University of Agricultural Sciences
en-keyword=Culture
kn-keyword=Culture
en-keyword=In vitro
kn-keyword=In vitro
en-keyword=In vivo
kn-keyword=In vivo
en-keyword=Lipid droplet
kn-keyword=Lipid droplet
en-keyword=Pig
kn-keyword=Pig
END
start-ver=1.4
cd-journal=joma
no-vol=10
cd-vols=
no-issue=4
article-no=
start-page=317
end-page=326
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2002
dt-pub=20020725
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Localisation of the hyaluronan receptor CD44 in porcine cumulus cells during in vivo and in vitro maturation
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Polyspermy is fairly common during porcine in vitro fertilisation (IVF), perhaps due to incomplete in vitro oocyte maturation (IVM). Porcine cumulus cells (CCs) layered around the oocyte produce large amounts of extracellular hyaluronan (HA) when forming an expanding cell cloud during the last phase of oocyte maturation. The specific actions of HA are mediated via HA-binding proteins (HABPs), such as CD44, which act as receptors. In this study using immunocytochemistry and western blotting we investigated the localisation of CD44 in CCs obtained from in vivo-matured pig cumulus?oocyte complexes (COCs) and compared it with that in CCs from immature COCs and of COCs subjected to IVM and IVF procedures. Immunolabelling of CD44 was absent or very weak in CCs from immature COCs but strongly present on the surface of the CCs obtained from in vivo, displaying a similar localisation in the in vitromatured
COCs. In the latter, the labelling decreased but did not disappear in CCs 4 h after sperm co-incubation during IVF. Immunoblotting detected bands of between 73 and 88 kDa, corresponding to CD44, in the protein extract from in vivo CCs collected immediately prior to, or following spontaneous ovulation. The in vitro-matured CCs, however, presented bands ranging from 81 kDa to 88 kDa. Also, the bands found in the in vivo-matured CCs showed a larger variation of intensity and migration among animals than did the batches of in vitro-matured CCs. No CD44 band was detected on aliquots of the frozenthawed boar spermatozoa used for IVF. The results clearly demonstrate that the specific HA receptor CD44 is present in expanding CCs of in vivo-matured pig COCs, in relation to increasing amounts of inter-CC HA. The subtle differences in molecular weight and migration ability observed between in vivo and in vitro samples may relate to differences in glycosylation and thus explain differences in HA-binding ability, of consequence for optimising in vitro culture conditions.
en-copyright=
kn-copyright=
en-aut-name=YokooMasaki
en-aut-sei=Yokoo
en-aut-mei=Masaki
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=1
ORCID=
en-aut-name=TienthaiPaisan
en-aut-sei=Tienthai
en-aut-mei=Paisan
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=2
ORCID=
en-aut-name=KimuraNaoko
en-aut-sei=Kimura
en-aut-mei=Naoko
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=3
ORCID=
en-aut-name=NiwaKoji
en-aut-sei=Niwa
en-aut-mei=Koji
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=4
ORCID=
en-aut-name=SatoEimei
en-aut-sei=Sato
en-aut-mei=Eimei
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=5
ORCID=
en-aut-name=Rodriguez-MartinezHeriberto
en-aut-sei=Rodriguez-Martinez
en-aut-mei=Heriberto
kn-aut-name=
kn-aut-sei=
kn-aut-mei=
aut-affil-num=6
ORCID=
affil-num=1
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=2
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=3
en-affil=
kn-affil=Swedish University of Agricultural Sciences
affil-num=4
en-affil=
kn-affil=Okayama University
affil-num=5
en-affil=
kn-affil=Tohoku University
affil-num=6
en-affil=
kn-affil=Swedish University of Agricultural Sciences
en-keyword=CD44
kn-keyword=CD44
en-keyword=Cumulus cells
kn-keyword=Cumulus cells
en-keyword=Hyaluronan (HA)
kn-keyword=Hyaluronan (HA)
en-keyword=Oocyte maturation
kn-keyword=Oocyte maturation
en-keyword=Pig
kn-keyword=Pig
END
start-ver=1.4
cd-journal=joma
no-vol=75
cd-vols=
no-issue=4-6
article-no=
start-page=389
end-page=406
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1963
dt-pub=19630630
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Studies on Seminal Vesiculogram in Male Sterility
kn-title=男子不妊症における精嚢腺レ線像の研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=Seminal vesicles were atrophied or not developed according to the lowering of the androgenic activities of the testis an I found tuberculous infection in them frequently. And it is well-known such conditions result in the disturbance of the sperm nutrition and the sperm passage which lead to the male sterility respectively. From these points, I have been, for the past seven years, observing the rentogenologic forms of semlnal vesicles about male sterility of 106 cases, and found out their morphological variations. On the classifications of seminal vesiculograms, there have been many reports, but could not be found that of seminal vesiculograms about male sterility. Then, it is intended to classify the forms of seminal vesicles of my cases as follows. 1. great main tubes with large laminated diverticula 2. great main tubes with or without small diverticula 3. simple main tubes with large laminated diverticula 4. simple main tubes with or without small diverticula 5. main tubes, dilated abnormally, demarcated poorly or irregularly, and no shaped seminal vesicles by means of the difficulties in injection of contrast medias into the vas deferens (stricture, obstruction or absence of it) The writer utilized vasopuncture of scrotal region and through the vas deferens injected Urografin and Urokolin-M into seminal vesicles. As table (2-16) shows, in seminal vesicles type No. 1 of the classification commanded a plurality (49.5%), Type No. 2 is the second (28.3%) and in Ampulla ductus deferentis, type No. 1 is first in number (60.6%) and type No. 2 next (19.2%). Seminal vesicles in male sterility are well-grown, and the angle of inclination of them have a tendency to be small (on an average 27.3 degree). Author's original classification of type No. 5 is 5.5%. But tuberculous findings in seminal vesicles are seen in few cases, on the contrary the abnormal dilatation of main tubes found frequently.
en-copyright=
kn-copyright=
en-aut-name=NambaKatsuichi
en-aut-sei=Namba
en-aut-mei=Katsuichi
kn-aut-name=難波克一
kn-aut-sei=難波
kn-aut-mei=克一
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学医学部泌尿器科教室
END
start-ver=1.4
cd-journal=joma
no-vol=78
cd-vols=
no-issue=7-9
article-no=
start-page=929
end-page=942
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1966
dt-pub=19660930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=Echinostoma hortense ASADA, 1926の生殖細胞染色体と受精に関する研究
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The adult worms were collected from rats 30 days after infection with metacercariae of Echinostoma hortense which cysted on the gill of Misgurunus anguillicaudatus. The testis and ovaries of matured worms were extracted from live adult worms and there were stained with 45% aceto-orcein solution for chromosome observations. The results of the study are briefly presented as follows.
1. Ten bivalent chromosomes can be seen in both primary spermatocytes and oocytes, which signifies the diploid number of chromosomes to be 20 (2n=20).
2. A spermatocyte is divided into four spertides after binary division.
3. The spermatozac after being transformed into the same number of spermatides, pass through vas deferens and wanter out to the seminal vesicle, and after copulation there, they are ejected into the uterus.
4. When the ovum has moved to the center of ovary, it reaches the leptotene stage and is inseminated.
5. The cocyte after insemination enters into egg-shell at ootype and then migranting towards the uterus, under goes two mitotis (meitotic) division and is transformed to prenucleus.
6. Sperms are gradually enlarged within the oocyte and forming the nucleus, now are transformed to sexual pronucleus. Oocytes that have formed sexual pronucleus in the center of uterus undergo karyogamy near she periphery of uterus, and after the first cleavage of ovum, there are formed two cells of a large and a small one each having 2n=20 chromosomes. Subsequently, they undergo the second cleavage to form one large cell and three small cells. At the time of spawning the ovum is composed of the primary and secondary polocytes and a cell at the fourth cleavage as well as a vitelline cell just before disintegration.
en-copyright=
kn-copyright=
en-aut-name=KusauraTsutomu
en-aut-sei=Kusaura
en-aut-mei=Tsutomu
kn-aut-name=草浦勉
kn-aut-sei=草浦
kn-aut-mei=勉
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学医学部寄生虫学教室
END
start-ver=1.4
cd-journal=joma
no-vol=118
cd-vols=
no-issue=1
article-no=
start-page=23
end-page=31
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20060501
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=
kn-title=メカノバイオロジー―基礎から臨床まで―
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=成瀬恵治
kn-aut-sei=成瀬
kn-aut-mei=恵治
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯薬学総合研究科 システム循環生理学
en-keyword=メカノトランスダクション
kn-keyword=メカノトランスダクション
en-keyword=ソフトリソグラフィー
kn-keyword=ソフトリソグラフィー
en-keyword=再生医療
kn-keyword=再生医療
en-keyword=不妊治療
kn-keyword=不妊治療
END
start-ver=1.4
cd-journal=joma
no-vol=120
cd-vols=
no-issue=1
article-no=
start-page=49
end-page=54
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2008
dt-pub=20080501
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Testicular fine needle aspirations cause histological injuryand immunological response in mouse testis
kn-title=マウス精巣吸引針生検後の精細管傷害および対精細胞遅延型過敏反応の解析
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The purpose of this study was to define the histopathological change and immunological response occurring as a result of testicular fine needle aspirations (TEFNAs) in C3H/HeN mice. TEFNA (one-, five-, or twenty-time aspiration) from the left testis was performed on day 0, and bilateral testes were examined histologically on days 21 and 49. Delayed-type hypersensitivity (DTH) was measured as the immunological response. Significant reduction of left testicular weight was observed only in the twenty-time aspiration group, on days 21 and 49. Histological examinations in the left testes showed a significantly high incidence of spermatogenetic disturbance with focal chronic inflammation and degenerative changes on day 21 and 49 after twenty-time TEFNA. Similar but less extensive changes were noted after five-time TEFNA. As for the weight and histology of the right testes, there was no significant change after the left-side TEFNA treatments. However, we revealed that the DTH response to autologous testicular cells was significantly elevated in the twenty-time aspiration group on day 21. In this animal model, TEFNA caused definite damage to the seminiferous tubules and induced DTH against testicular cells. Further study may be required to determine the clinical relevance of such findings to the testes of azoospermic men treated with TEFNA.
en-copyright=
kn-copyright=
en-aut-name=TsuboiHiromu
en-aut-sei=Tsuboi
en-aut-mei=Hiromu
kn-aut-name=坪井啓
kn-aut-sei=坪井
kn-aut-mei=啓
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学大学院医歯学総合研究科 泌尿器病態学
en-keyword=精巣生検（testicular biopsy）
kn-keyword=精巣生検（testicular biopsy）
en-keyword=精巣損傷（testicular injurly）
kn-keyword=精巣損傷（testicular injurly）
en-keyword=精子形成（spermatogenesis）
kn-keyword=精子形成（spermatogenesis）
en-keyword=遅延型過敏反応（delayed-type hypersensitivity）
kn-keyword=遅延型過敏反応（delayed-type hypersensitivity）
en-keyword=自己免疫性精巣炎（autoimmune orchitis）
kn-keyword=自己免疫性精巣炎（autoimmune orchitis）
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2006
dt-pub=20060324
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=加熱乾燥精子による哺乳類卵子の顕微受精に関する研究
kn-title=STUDIES ON FERTILIZATION OF MAMMALIAN OOCYTES FOLLOWING INTRACYTOPLASMIC INJECTION OF HEAT-DRIED SPERM HEADS
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=李庚本
kn-aut-sei=李
kn-aut-mei=庚本
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2004
dt-pub=20040930
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=精子侵入直後のラット卵子の培養に関する研究
kn-title=STUDIES ON CULTURE OF RAT OOCYTES SOON AFTER SPERM PENETRATION
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=YangXin-Zhi
en-aut-sei=Yang
en-aut-mei=Xin-Zhi
kn-aut-name=楊信志
kn-aut-sei=楊
kn-aut-mei=信志
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1997
dt-pub=19970325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=ブタ体外受精卵子の雄性前核形成に関する研究
kn-title=Studies on Male Pronuclear Formation after In Vitro Fertilization of Pig Oocytes
en-subtitle=
kn-subtitle=
en-abstract=家畜こおける初期肺の体外生皮技織の確立は、優良な遺伝形質を持つ個体を効率的に増産する手段として極めて重要である。しかし、ブタでは体外成熟卵子を体外受精した場合、多精侵入が高率に起こり、さらに卵子内に侵入した精子の雄性前核(MPN)形成率が低いため、正常な1-細胞期胚を体外で作出することは困難である。最近、高濃度(0.57mM)のシステインを添加した成熟培地でブタ卵子を培養した場合、受精後のMPN形成の促進されることが報告されたが、その作用機序の詳細は知られていない。そこで本研究では、MPN形成に対するシステインの作用機構を明かにする目的で、成熟過程におけるシステインと他の幾つかの要因がブタ体外受精卵子のMPN形成におよぽす影平について検討し、次のような新しい知見を得た。I. 成熟途上ブタ卵子のMPN形成におよぼすシステインの影響 ブタでは卵子成熟の各段階で精子侵入の可能なことが報告されているが、このような未熟侵入卵子ではMPNはほとんど形成されない。そこで本実験では、システインの存在下で各成熟段階に到達した卵子のMPN形成能について検討した。ブタ卵子をシステイン(0.57mM)添加または無添加の修正TCM-199B(mTCM-199B)で0-48時間培養した。培養開始後12時間ではほとんど全ての卵子(98-100%)が卵核胞期にとどまっていたが、その後、培養の継続にともなって核成熟は進行し、培養開始後48時間では88-90%の卵子が第二減数分裂中期に到通した。卵子内のグルタチオン(GSH)濃度は、システインを添加した場合、培養後24時間の卵子(10.5mM)において培養開始前の卵子(6.7mM)よりも有意に高い値を示し、その値は培養開始後48時間まで維持されたが、システイン無添加では、GSH濃度の増加はみられず、逆に培養開始後48時間でのGSH濃度(4.3mM)は有意に減少した。培養開始後24、36および48時間の卵子を体外受精(IVF)した結果、培養開始後24時間で侵入した卵子の活性化率(22%)は低かったが、活性化された卵子におけるMPN形成率はシステイン添加(71%)において無添加(22%)よりも砧かった。一方、培養開始後36および48時間における侵入卵子の活性化率は83-100%と高く、MPN形成率はシステイン添加(84-85%)において無添加(23-36%)と比較して有意に高かった。システインを添加した成熟培地で24時間培養した卵子をシステイン添加または無添加の培地でIVFした結果、卵子の活性化およびMPN形成率に差はみられなかった。受精14時間後にこれらの卵子をシステイン無添加の成熟培地に移し24時間追加培養した結果、卵子の活性化は促進されなかったが、中期像染色体を形成した精子核が観察された。II. システイン添加の時期および期間がブタ卵子のGSH合成およびMPN形成におよぽす影響 本実験では、MPN形成の重要な要因と考えられているGSH合成に影響をおよぼすシステイン添加の種々の条件について検討した。システイン(0.57mM)を添加したmTCM-199Bで0-36時間培養した後、無添加培地に移し48時間後まで培養を継続した卵子のMPN形成率(31-75%)およびGSH濃度(4.0-7.2mM)は､全培養期間中システインが存在していた場合(それぞれ、90%および11.5mM)と比較して有意に低かった。一方、培養開始後36時間に至るいずれの時間にシステインを添加しても、MPN形成率(85-92%)およびGSH濃度(10.2-11.9mM)は高かった。システインの添加時期が培養開始後36-42時間の範囲では、添加時期の相違によるMPN形成率(86-90%)に差はみられなかったが、培養開始後45時間でシステインを添加するとMPN形成率(60%)は有意に低下した。培養開始後42および45時間から3時間のみシステインを添加した場合、MPN形成準(65-70%)は、培養開始後42時間から培養終了までシステインが存在した場合(92%)と比較して有意に低かったが、全培養期間無添加の場合(28%)と比較して高かった。また、卵子のGSH濃度は、培養開始後36、39および42時間から培養終了までシステインを添加することによって(8.9-11.4mM)、42および45時間から3時間のみシステインを添加ルた域合(3.4-3.8mM)と比較して高くなった。III. システインあるいはシスチンの存在下で成熟したブタ卵子のGSH合成およびMPN形成におよぽす卵丘細胞の影響 本文験では、システインおよびシスチン(酸化型システイン)のブタ卵子による利用性と卵丘細胞との関連性について検討した。システイン(0.57 mM)もしくはシスチン(0.57mM)を添加または無添加のmTCM-199Bで卵丘細胞付着卵子を48間培養した結果、IVF後のMPN形成率および卵子内のGSH濃度は、システイン(それぞれ、86%および7.6 mM)もしくはシスチン(それぞれ､81%および8.2mM)を添加した場合、無添加(それぞれ、38%および3.0mM)と比較して有意に高かった。卵丘細胞付着卵子をシステインおよびシスチン無添加の培地で24時間培養後、一部の卵子から卵丘細胞を除去し、それらをシステインもしくはシスチン添加または無添加の培地に移し48時間まで培養した結果、システイン添加培地においては卵丘細胞の付着の有無にかかわらずMPN形成率(79-90%)は高かった。シスチン添加培地では卵丘細胞付着卵子のMPN形成のみ促進され(90%)、卵丘細胞除去卵子のMPN形成率(16%)は全培養期間システインおよびシスチン無添加の卵子におけるそれ(22%)と差がなかった。これらの卵子のGSH濃度はMPN成率に比例した。システインもしくはシスチンの添加および卵丘細胞の除去を培養開始36時間に行なった結果、MPN形成率は、システインを添加した場合においては卵丘細胞付着の有無にかかわらず高かったが(79-92%)、シスチン添加では卵丘細胞付着および除去卵子のいずれにおいても低かった(14-29%)。これらの結果から、1)ブタ卵子内のGSH濃度が高くなることによって、侵入した精子核の十分な脱濃縮が誘起され、それによってMPN形成が促進されるが、これらの課程は卵子の活性化と密接に関連していること、2)卵子核成熟が第一成熟分裂中期から第二成熟分裂中期に至る成熟過程の後半時期(培養開始後42-48時間)にのみシステインが存在することによって、MPN形成に十分なGSHが合成されること、3)卵丘細胞除去卵においてもシステインによるGSH合成は可能であるが、シスチンによるGSH合成には卵丘細胞の存在が必要である、ことが示唆された。
kn-abstract=The objective of the present study was to examine the effects of various factors responsible for male pronuclear (MPN) formation associated with glutathione (GSH) synthesis of pig oocytes. In the first series of experiments, when cumulus-enclosed oocytes were cultured for 24 h in maturation medium supplenlented with 0.57 mM cysteine, GSH concentration of oocytes was increased. However, the activation of oocytes after sperm penetration in vitro was inhibited and thereby MPN formation of the oocytes was not promoted. The MPN formation in activated oocytes after sperm penetration at any stages of maturation was promoted when cysteine was added to maturation medium. The results indicate that an increased concentration of GSH and intimate synchronization with oocyte activation are essential for MPN formation of pig oocytes. In the next series of experiments, it was shown that, when cysteine was removed from maturation medium at 36 h of culture, both the incidence of MPN formation after in vitro fertilization and GSH concentration of lmatured oocytes were lower than when cysteine was present during whole period of culture for 48 h. In contrast, the presence of cysteine in maturation medium only bc~tween 42 and 48 h of culture, when oocytes reached to the late metaphase-I to metaphase-II stage, could promote oocyte GSH synthesis and thereby MPN formation after in vitro fertilization. The results indicate that the presence of cysteine in maturation medium for 6 h of the last phase of maturation is essential for oocyte GSH synthesis enough to induce MPN formation with the same efficiency as in oocytes matured in the presence of cysteine from the start of culture. The effects of cystine, an oxidized form of cysteine, added to maturation medium at various times after culture on the MPN formation and GSH synthesis of cumulus-enclosed and cumulus-free oocytes were examined in the third series of experiments. When maturation medium was supplemented with 0.57 mM cystine, MPN formation and GSH synthesis of cumulus-enclosed oocytes were accelerated, but not in cumulus-denuded oocytes. On the other hand, cysteine (0.57 mM) could promote MPN formation and GSH synthesis in both cumulus-enclosed and cumulus-denuded oocytes. The results indicate that cystine is associated with increased GSH synthesis and MPN formation of pig oocytes and that the presence of cumulus cells is essential for the utilization of the cystine by oocytes. In contrast, cysteine is utilized directly by oocytes without cumulus cells for GSH synthesis and MPN formation. Taken together, it is concluded from these results that synchronization of oocyte activation and sperm penetration, and the addition of exogenous compounds such as cysteine or cystine to maturation medium are important factors for GSH synthesis of matured oocytes and MPN formation of oocytes following fertilization. The presence of cumulus cells associated with utilization of cystine by oocytes is also an important factor.
en-copyright=
kn-copyright=
en-aut-name=SawaiKen
en-aut-sei=Sawai
en-aut-mei=Ken
kn-aut-name=澤井健
kn-aut-sei=澤井
kn-aut-mei=健
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1999
dt-pub=19990325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=豚の配偶子間結合における透明帯糖残基および精子グリコシダーゼの役割に関する研究
kn-title=STUDIES ON PARTICIPATION OF SUGAR RESIDUES OF ZONA PELLUCIDA AND SPERM GLYCOSIDASES IN IN-VITRO SPERM-OOCYTE INTERACTION IN PIGS
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=宋学雄
kn-aut-sei=宋
kn-aut-mei=学雄
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1994
dt-pub=19940325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=牛成熟途上卵子に侵入した精子核の形態的変化に関する研究
kn-title=STUDIES ON SPERM NUCLEAR TRANSFORMATION IN MATURING BOVINE OOCYTES IN VITRO
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=
en-aut-sei=
en-aut-mei=
kn-aut-name=アベイデーララランタ ロハーナ
kn-aut-sei=アベイデーラ
kn-aut-mei=ラランタ ロハーナ
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=
cd-vols=
no-issue=
article-no=
start-page=
end-page=
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=2003
dt-pub=20030325
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=卵細胞質内精子注入によるラット卵子の受精に関する研究
kn-title=Studies on fertilization of rat oocytes by intracytoplasmic sperm injection
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=
en-copyright=
kn-copyright=
en-aut-name=SaidSyahruddin
en-aut-sei=Said
en-aut-mei=Syahruddin
kn-aut-name=サイドサハルディン
kn-aut-sei=サイド
kn-aut-mei=サハルディン
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
END
start-ver=1.4
cd-journal=joma
no-vol=39
cd-vols=
no-issue=1
article-no=
start-page=1
end-page=8
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1972
dt-pub=1972
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=放射線によるハッカ属植物の育種学的基礎研究 (第13報)ハッカの花粉におよぼす放射線の影響
kn-title=Studies on the Radiation Breeding in the genus Mentha : (XIII)Effects of Irradiation on Pollen
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=The effects of pollen grain irradiation on pollen-tube mitosis were investigated. The data were obtained from artificial culture. For irradiation gamma-rays were applied with 60Co at a dose rate 65 KR/hr. The doses ranged from 1 to 80 KR. 1) Inhibition of mitosis increased with increasing dose; namely the higher the dose, the more reduced was the frequency of nuclear division and the more delayed was the time of its occurrence. 2) In M, rotundifolia the generative nucleus remained mostly undiveded in showing chromosome elements even at 5KR, while at 20KR a few nuclei consisted of chromatin masses or chromosomes. The pollen-tubes of M. rotundifolia did not grow smoothly on the media. In natural condition, therefore, their pollen-tube mitosis may be more radio-resistant. 3) At 80 KR irradiation of M. spicata pollen the mitotic division of the generative nuoleus was almost completely inhibited. 4) In M. arvensis the generative nucleus did not divide into 2 sperm nuclei above 25 KR, while relatively many generative nuclei with chromosomes were observed at 50 KR. 5) The pollen grains of M, arvensis L. var. piperascens had the highest radioresistance. At 50-80KR the generative nucleus underwent complete mitotic division. 6) Radio-sensitivity of pollen grains varied widely according to the species. In general, their pollen-tube mitosis was much more radio-resistant than the meiosis in PMC's and mitosis in root tips.
en-copyright=
kn-copyright=
en-aut-name=OnoSeiroku
en-aut-sei=Ono
en-aut-mei=Seiroku
kn-aut-name=小野清六
kn-aut-sei=小野
kn-aut-mei=清六
aut-affil-num=1
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
en-keyword=ハッカ属植物
kn-keyword=ハッカ属植物
en-keyword=育種学
kn-keyword=育種学
en-keyword=花粉
kn-keyword=花粉
END
start-ver=1.4
cd-journal=joma
no-vol=38
cd-vols=
no-issue=1
article-no=
start-page=9
end-page=14
dt-received=
dt-revised=
dt-accepted=
dt-pub-year=1971
dt-pub=1971
dt-online=
en-article=
kn-article=
en-subject=
kn-subject=
en-title=Experimental Studies on the Foundations of Sterile Male Technique of Spodoptera littralis(B), by the Gamma Radiation form 137Cs : I. Anatomical Observations on the Mating, Transfer of sperm and Fecundity of Normal Females paired with Irradiated Males
kn-title=137CsによるハスモンヨトウSpodoptera littralis(B)のSterile Male Techniqueのための基礎研究 1.γ線を照射した雄と交配させた雌の交尾・精子移動および産卵率に関する解剖観察
en-subtitle=
kn-subtitle=
en-abstract=
kn-abstract=本実験は137Csガンマー線12KRを用い,蛹時代に照射された雄を無照射雌と交配させた際の雌の不姙現象および,その一部より孵化した2代目雄と無照射区の2代目雌の交配の際のInherited sterilityと呼ばれる不姙現象の原因を,雄・雌生殖器解剖によって検討したものである. 1.照射区の雄と無照射雌の交配における雌の不姙は,ガンマー線による精子の致死変異に起因するものより,むしろ雄生殖系の組織の機能異常に基づく不完全交尾や精子が精莢中へ挿入されないこと,あるいは精子の活動力の低下による場合が多かった. 2.それら組み合わせから孵化し得た幼虫の精巣を解剖してみると,一般にその発育がよくないものが多かった. 3.上記の幼虫が発育し羽化したとき無照射雄と交配すると完全なInherited sterilityが認められた. その原因は生殖細胞の染色体異常によるよりも雄の精巣の発育不全に基ずく不完全交尾や精子の移動機能の欠除に基ずく雌受精嚢内の無精子に起因する場合が多かった。
en-copyright=
kn-copyright=
en-aut-name=TsukudaRitsuko
en-aut-sei=Tsukuda
en-aut-mei=Ritsuko
kn-aut-name=佃律子
kn-aut-sei=佃
kn-aut-mei=律子
aut-affil-num=1
ORCID=
en-aut-name=KiyokuMasao
en-aut-sei=Kiyoku
en-aut-mei=Masao
kn-aut-name=清久正夫
kn-aut-sei=清久
kn-aut-mei=正夫
aut-affil-num=2
ORCID=
affil-num=1
en-affil=
kn-affil=岡山大学
affil-num=2
en-affil=
kn-affil=岡山大学
END