Herpetological Society of Japan Acta Medica Okayama 1345-5834 38 1 2019 Individual and Sexual Differences in Time to Habituate to Food-stimuli Presentation of Potential Prey in Hyla japonica 14 22 EN Shintaro Tanabe Graduate School of Environmental and Life Science, Okayama University Eiiti Kasuya Department of Biology, Faculty of Sciences, Kyushu University Takahisa Miyatake Graduate School of Environmental and life Science, Okayama University The traits of many animal species exhibit individual and sexual differences. Individuals repeatedly receiving a stimulus without harm become habituated to it. However, few studies have been conducted on individual and sexual differences in the process of habituation to unfamiliar food stimuli. Therefore, we hypothesized that individual differences or sexual differences would be observed in reaction to an in-lab food-stimuli presentation of potential prey items (after that "food stimuli"). We tested the hypothesis using the Japanese tree frog Hyla japonica, and conducted statistical analyses of these results. A generalized linear model (GLM) showed individual and sexual differences in time to get used to the food stimuli. Females habituated more rapidly to food stimuli than males. The difference between sexes is discussed in view of two ultimate and one proximate reasons. No potential conflict of interest relevant to this article was reported.