| タイトル（別表記） | Identification of Marker Gene of Pars Tuberalis Morphogenesis in Chicken Embryo. The expression of Cytokine-like 1 and Gap junction protein alpha 5 in the pars tuberalis |
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| フルテキストURL | poalas_033_031_034.pdf |
| 著者 | 相澤 清香| 檜垣 佑理子| 御輿 真穂| 竹内 栄| 高橋 純夫| |
| 抄録 | Adenohypophysis delivered from oral ectoderm consists of pars distalis (PD), pars intermedia and pars tuberalis (PT). The mechanisms of development of PD has been well studied, and the cell differentiation of PD has been well understood. However, the morphogenesis and the differentiation of PT are still unclear, and the gene expression during the PT development remains largely unknown. In this study, we explored the specifically expressing genes in PT during development and analyzed its spatiotemporal expressions pattern. Microarray analysis on laser-captured PT and PD tissues obtained from chicken embryos on embryonic day 10 (E10.0) showed high expressing genes, Cytokine-like 1 (CYTL1) and Gap junction protein alpha 5 (GJA5) in PT. A detail analysis of spatiotemporal expressions pattern during chick embryo development by in situ hybridization revealed that CYTL1 mRNA was first detected in lateral head ectoderm and ventral head ectoderm in E1.5. The CYTL1 expressions moved into Rathke’s pouch at E2.5, then it was localized in PT primordium and continuously expressed in PT primordium until E12.0. On the other hand, GJA5 mRNA was transiently detected in PT primordium from E6 to E14.0, while the expression was not detected in PD during development. These results suggested that these genes may be involved in the regulation mechanism of PT development and could be a useful marker in the PT development. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 31 |
| 終了ページ | 34 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Structure and function of tegmentum vasculosum in avian cochlea |
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| フルテキストURL | poalas_033_026_030.pdf |
| 著者 | 池田 理佐| 乙野 剛史| 池田 直矢| 齋藤 昇| 安藤 元紀| |
| 抄録 | In spite of the importance of endocochlear DC potential (EP) and the K+-rich endolymph for the avian cochlea, the structure and function of the tegmentum vasculosum (TV) has not yet been fully elucidated, compared with those of the stria vascularis in the mammalian cochlea. In this study, we examined structural analysis of the epithelial cells, gene expressions of Na+-K+-ATPase (Atp1A1) and Kir4.1 (Kcnj10), and protein localizations of Na+-K+-ATPase and Kir4.1, in the TV. Tight junctional structures were observed between epithelial dark cells and light cells in the TV. Both Atp1a1 and Kcnj10 genes were detected in the TV. In addition, immunopositive signals for both Na+-K+-ATPase and Kir4.1 were recognized in the TV. These results indicate that Na+-K+-ATPase and Kir4.1 play roles in maintaining the EP and high K+ concentration of the endolymph. Further studies are needed to clarify the physiological functions of the TV. This is the first report which demonstrates that gene and protein expression data contribute to the avian inner ear homeostasis. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 26 |
| 終了ページ | 30 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Taxonomic status of Aco strain derived from spiny mice |
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| フルテキストURL | poalas_033_022_025.pdf |
| 著者 | 持田 瞳| 目加田 和之| |
| 抄録 | Spiny mice are useful in regenerative research and as a model for precocial animals. In the present study, we investigated the taxonomic status of the Aco strain, which is derived from spiny mice of an unidentified species. Although this Aco strain has been tentatively considered A. cahirinus species, it was distinguished from this species based on conventional and G-banding chromosomal patterns of this strain. Subsequently, we determined the nucleotide sequence of the mitochondorial gene cytochrome b (Cyb) and constructed a molecular phylogenetic tree. We found that the Aco strain belongs to the same clade as A. dimidiatus. Based on this molecular analysis, we concluded that the Aco strain of spiny mice should be considered A. dimidiatus. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 22 |
| 終了ページ | 25 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Inhibitory effects of caffeine on allergic rhinitis symptoms in mice |
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| フルテキストURL | poalas_033_018_021.pdf |
| 著者 | 辻本 まどか| 佐伯 綾希子| 鈴木 真奈美| 白神 俊幸| 杉本 幸雄| 林 泰資| |
| 抄録 | We examined the effects of caffeine treatment on ovalbumin- and histamine-induced rhinitis mouse models and on corticosterone and catecholamine levels in mouse plasma. Treatment with caffeine decreased sneezing and nasal rubbing in both models, but direct nasal administration had no effect. Plasma levels of corticosterone and catecholamine, which have known anti-allergy effects, were increased by caffeine treatment. The increases in corticosterone and catecholamine may be associated with the suppression of rhinitis symptoms. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 18 |
| 終了ページ | 21 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Dissecting the hierarchy and lineage of mesenchymal stem cells by mouse genetics |
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| フルテキストURL | poalas_033_015_017.pdf |
| 著者 | 宝田 剛志| |
| 抄録 | Determination of stem cell hierarchy/lineage is indispensable for a better understanding and augmentation of many aspects of medical sciences, including the mechanisms of tissue development and maintenance of tissue homeostasis, as well as disease development. It also has implications in the field of tissue regeneration for medical treatments and disease modeling for drug discovery using iPS technology. Mesenchymal stem cells are multipotent stem cell that can differentiate into various type of cells including osteoblasts, adipocytes, myocytes and chondrocytes. Runt-related transcription factor 2 (Runx2) is an essential transcriptional regulator of osteoblast differentiation. Runx2 deficiency in Prx1+-derived cells (Runx2prx1−/− mice) resulted in defective intramembranous ossification. Double-positive cells for Prx1-GFP and stem cell antigen-1 (Sca1) (Prx1+Sca1+ cells) in the calvaria expressed Runx2 at lower levels and were more homogeneous and primitive as compared with Prx1+Sca1− cells. Our results suggest that osteoblast differentiation in vivo may begin at the Prx1+Sca1+ MSC stage with sequential progression to Prx1+Sca1−cells, then Osx+Prx1−Sca1− osteoblast precursors, which eventually form mature α1(I)-collagen+ osteoblasts. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 15 |
| 終了ページ | 17 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Contribution and progress of the gnotobiote technology in gut microbiome research |
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| フルテキストURL | poalas_033_011_014.pdf |
| 著者 | 森田 英利| |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 11 |
| 終了ページ | 14 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Overview and future perspectives of studies on the mechanisms underlying appetite regulation in chickens |
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| フルテキストURL | poalas_033_008_010.pdf |
| 著者 | 本田 和久| |
| 抄録 | Broiler chickens eat more feed than layer chickens. As a result, broiler chickens grow faster than layer chickens. However, excessive accumulation of body fat in broiler chickens has been a serious problem in the poultry industry in recent decades. Therefore, the appetite regulatory system of chickens has been a focus of research among poultry scientists. Lines of evidence suggest that the physiological role of peripheral adiposity hormones, such as leptin and insulin, and gut hormones, such as ghrelin, are different between mammals and chickens. Thus, species specificity make it difficult to understand the mechanisms underlying the regulation of food intake in chickens. Here I provide an overview of recent findings in this field and future perspectives. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 8 |
| 終了ページ | 10 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Pharmacokinetic analysis of drug absorption following nasal application to rats |
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| フルテキストURL | poalas_033_003_007.pdf |
| 著者 | 古林 呂之| |
| 抄録 | Since a drug applied to the nose in an in vivo physiologic condition is translocated to the gastrointestinal (GI) tract by mucociliary clearance (MC), the drug undergoes absorption both from the nasal cavity and from the GI tract. The detailed MC of the rat was examined, using inulin as a marker of the applied solution. Inulin disappeared monoexponentially from the nasal cavity, indicating that the MC can be assumed to follow first-order kinetics. In the proposed kinetic model, the fractional absorption of the drug following nasal application is predicted as the sum of fractional absorption from the nasal cavity and fractional absorption from the GI tract, both of which are estimated indirectly from the permeability to the Caco-2 monolayer. This kinetic model is the first estimation system for nasal drug absorption based on drug disposition after nasal application and is useful for the development of nasal dosage forms. |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 3 |
| 終了ページ | 7 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | Breeding and genetics of mutant strains and domestication of wildlife for laboratory animal science |
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| フルテキストURL | poalas_033_002.pdf |
| 著者 | 織田 銑一| |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 2 |
| 終了ページ | 2 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| タイトル（別表記） | A greeting from President of Okayama Association for Laboratory Animal Science |
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| フルテキストURL | poalas_033_001.pdf |
| 著者 | 国枝 哲夫| |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 開始ページ | 1 |
| 終了ページ | 1 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | poalas_033_contents.pdf |
|---|---|
| 著者 | 岡山実験動物研究会| |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| フルテキストURL | poalas_033_contents_eng.pdf |
|---|---|
| 著者 | 岡山実験動物研究会| |
| 出版物タイトル | 岡山実験動物研究会報 |
| 発行日 | 2017-04 |
| 巻 | 33巻 |
| 言語 | 日本語 |
| 論文のバージョン | publisher |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 岡山実験動物研究会| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 木村 透| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | その他 |
| 著者 | 矢田 範夫| 上藤 千佳| 平山 晴子| 樅木 勝巳| |
|---|---|
| 発行日 | 2016-04 |
| 出版物タイトル | 岡山実験動物研究会報 |
| 巻 | 32巻 |
| 資料タイプ | 学術雑誌論文 |